FastQCFastQC Report
Thu 26 May 2016
SRR938066_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938066_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988732
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103410.519979564868469No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83840.4215751544200023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54260.27283716458527346No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA61550.041.3441731
GTATCAA97500.036.0403021
TATCAAC119400.029.1380582
ATCAACG119650.029.1176323
TCAACGC121550.028.6624834
CAACGCA122200.028.432235
AACGCAG125900.027.6686746
GTACATG89950.025.477411
ACGCAGA141950.024.46657
TACATGG90100.024.370872
CGCAGAG143600.023.9537778
GAGTACT76650.023.21086512-13
ACATGGG93200.022.3369033
GCAGAGT152750.022.0495789
GTATAGA5900.021.8487661
GTACTTT82800.021.48767914-15
AGTACTT80800.020.46065512-13
GGTATAG3553.6379788E-1220.1733531
AGAGTAC125600.020.12135710-11
CAGAGTA146600.019.41011410-11
Produced by FastQC (version 0.11.5)