##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938066_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1988732 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.733741399042202 33.0 31.0 34.0 30.0 34.0 2 31.932104979454245 33.0 31.0 34.0 30.0 34.0 3 32.00673745884312 34.0 31.0 34.0 30.0 34.0 4 35.53507058769105 37.0 35.0 37.0 33.0 37.0 5 35.54403157388728 37.0 35.0 37.0 33.0 37.0 6 35.56682398633903 37.0 35.0 37.0 33.0 37.0 7 35.50275653029166 37.0 35.0 37.0 33.0 37.0 8 35.476764088876735 37.0 35.0 37.0 33.0 37.0 9 37.08501246020077 39.0 37.0 39.0 33.0 39.0 10-11 36.98243101634609 39.0 37.0 39.0 33.0 39.0 12-13 36.87744125402518 39.0 37.0 39.0 33.0 39.0 14-15 38.02884878404933 40.0 38.0 41.0 33.0 41.0 16-17 37.8799926284688 40.0 38.0 41.0 33.0 41.0 18-19 38.02394063151797 40.0 38.0 41.0 33.0 41.0 20-21 38.008550925916616 40.0 38.0 41.0 33.0 41.0 22-23 37.91083212821033 40.0 38.0 41.0 33.0 41.0 24-25 37.79651380879877 40.0 38.0 41.0 32.5 41.0 26-27 37.61407897092218 40.0 37.5 41.0 32.0 41.0 28-29 37.40192972205405 40.0 37.0 41.0 31.5 41.0 30-31 37.17950734437822 40.0 37.0 41.0 31.0 41.0 32-33 36.932481098509 39.5 36.0 41.0 30.5 41.0 34-35 36.69621572941955 39.0 36.0 41.0 30.0 41.0 36-37 36.43771785238031 39.0 35.0 41.0 30.0 41.0 38-39 36.15108722542806 39.0 35.0 40.0 29.5 41.0 40-41 35.940603862159406 39.0 35.0 40.0 29.0 41.0 42-43 35.752757535957585 39.0 35.0 40.0 27.5 41.0 44-45 35.472135511471635 39.0 35.0 40.0 26.5 41.0 46-47 35.06153594350571 38.0 34.5 40.0 25.0 41.0 48-49 34.66338978806596 38.0 34.0 40.0 24.0 41.0 50-51 33.968034405842516 37.5 33.5 39.5 23.0 40.5 52-53 34.11089955811039 38.0 33.5 40.0 23.0 40.5 54-55 34.22086359549703 38.0 34.0 40.0 23.0 41.0 56-57 33.921684269172516 38.0 33.5 40.0 21.5 41.0 58-59 33.60308075698485 37.5 33.0 40.0 20.0 41.0 60-61 33.18183495815424 37.0 33.0 40.0 18.5 41.0 62-63 32.63486658835881 36.5 32.0 39.5 14.0 41.0 64-65 32.10616111170334 36.0 31.0 39.0 9.0 40.5 66-67 31.566528320558024 35.0 31.0 39.0 7.5 40.0 68-69 30.961574510793813 35.0 30.0 38.0 2.0 40.0 70-71 30.40828628492929 35.0 29.5 37.5 2.0 40.0 72-73 29.76265806554126 34.0 29.0 37.0 2.0 39.0 74-75 29.0718349682109 34.0 28.0 36.0 2.0 39.0 76-77 28.387118274357732 34.0 26.5 36.0 2.0 38.5 78-79 27.653347711003796 33.0 26.0 35.0 2.0 37.0 80-81 26.972613454200967 33.0 25.0 35.0 2.0 37.0 82-83 26.239039749951225 32.0 23.0 35.0 2.0 36.0 84-85 25.574941470243353 32.0 20.5 35.0 2.0 36.0 86-87 24.86922747760885 31.0 18.0 34.0 2.0 35.0 88-89 24.062837526625003 31.0 10.0 34.0 2.0 35.0 90-91 23.22018275966797 30.5 4.0 34.0 2.0 35.0 92-93 22.306045510405625 29.5 2.0 34.0 2.0 35.0 94-95 21.338478487800266 29.0 2.0 34.0 2.0 35.0 96-97 20.123914635053893 28.0 2.0 34.0 2.0 35.0 98-99 18.635559492178935 25.5 2.0 33.5 2.0 35.0 100-101 15.10215252733903 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4885619443064435 1101 2 0.4457653823375871 1101 3 0.4082426504083543 1101 4 0.31990134397155856 1101 5 0.34981689914815917 1101 6 0.43403190889507925 1101 7 0.3880204845933548 1101 8 0.3978568949841943 1101 9 0.5215212109263234 1101 10-11 0.47259503004497105 1101 12-13 0.5588580908605607 1101 14-15 0.6952105994164341 1101 16-17 0.8028571436850314 1101 18-19 0.6722879697157822 1101 20-21 0.7319623039949406 1101 22-23 0.733046227453535 1101 24-25 0.8060820298933749 1101 26-27 0.7175314041293248 1101 28-29 0.865850277079204 1101 30-31 1.0071501535760845 1101 32-33 0.7288088001584541 1101 34-35 0.9356712187848615 1101 36-37 1.0096191749136523 1101 38-39 1.086886616284616 1101 40-41 1.0904416690817698 1101 42-43 1.1922650737879295 1101 44-45 1.2012173602080694 1101 46-47 1.4615470607323786 1101 48-49 1.6439089305020786 1101 50-51 1.590405067524216 1101 52-53 1.4811044155945225 1101 54-55 1.621519313066436 1101 56-57 1.6895237869012405 1101 58-59 1.6727263088870217 1101 60-61 1.757476465347402 1101 62-63 1.510310123878405 1101 64-65 1.5994786245408719 1101 66-67 1.706948434385044 1101 68-69 1.957545197175893 1101 70-71 2.172476138626447 1101 72-73 2.4143049778316588 1101 74-75 2.5613197474017255 1101 76-77 2.6297155680376996 1101 78-79 2.6485372584783775 1101 80-81 2.764085331089614 1101 82-83 2.92439654828328 1101 84-85 3.094511627892963 1101 86-87 3.223681291108619 1101 88-89 3.729237597903399 1101 90-91 3.817960955095888 1101 92-93 4.189631303364166 1101 94-95 4.357056660934692 1101 96-97 4.489053547562033 1101 98-99 4.743642010414103 1101 100-101 4.502482669274675 1102 1 0.5320575031960963 1102 2 0.4005369994961434 1102 3 0.4141235864688326 1102 4 0.28741029569695087 1102 5 0.4235332193299257 1102 6 0.43671023637244133 1102 7 0.2865933614645897 1102 8 0.3597785080683309 1102 9 0.5012713774979218 1102 10-11 0.4668123592431783 1102 12-13 0.5225466810656769 1102 14-15 0.6764056847154691 1102 16-17 0.700833538452521 1102 18-19 0.6416241159920091 1102 20-21 0.7739524271380773 1102 22-23 0.7540324511436154 1102 24-25 0.7626080414263612 1102 26-27 0.7710763832539484 1102 28-29 0.6271723522497297 1102 30-31 0.7817191645482424 1102 32-33 0.91779296507152 1102 34-35 0.8947692036280444 1102 36-37 0.9648230992925448 1102 38-39 1.0073993092799753 1102 40-41 1.232385835241132 1102 42-43 1.2039406642183295 1102 44-45 1.1258985373779282 1102 46-47 1.1643581661446376 1102 48-49 1.343032692000314 1102 50-51 1.241918412425683 1102 52-53 1.2124486963167982 1102 54-55 1.4235888153648801 1102 56-57 1.320966752307072 1102 58-59 1.393854897366495 1102 60-61 1.4615841928314737 1102 62-63 1.419321886948886 1102 64-65 1.455516125918983 1102 66-67 1.5069660502302007 1102 68-69 1.6740364443777231 1102 70-71 1.8836748429630568 1102 72-73 2.0772713183854883 1102 74-75 1.949133177626205 1102 76-77 2.099248544420483 1102 78-79 2.524846762885936 1102 80-81 2.4090553482021484 1102 82-83 2.7867619728131636 1102 84-85 2.71889177783137 1102 86-87 2.677111946993268 1102 88-89 2.9640169684305313 1102 90-91 3.1213947866260945 1102 92-93 3.4866587193713343 1102 94-95 3.6814827079390433 1102 96-97 3.941562367468176 1102 98-99 4.137378918365922 1102 100-101 4.040289628372358 1103 1 0.7698018033274359 1103 2 0.6357611213627585 1103 3 0.6231622703632596 1103 4 0.5459474697295761 1103 5 0.5921788901239182 1103 6 0.5410254102029981 1103 7 0.5933324528533248 1103 8 0.5771663348776386 1103 9 0.6823172795421328 1103 10-11 0.7281840322149691 1103 12-13 0.7361065310898098 1103 14-15 0.9466155218300045 1103 16-17 0.9488935905399529 1103 18-19 0.9251069855152494 1103 20-21 0.9742315270583859 1103 22-23 1.0317712506631551 1103 24-25 1.1755805046901315 1103 26-27 1.1009433021255361 1103 28-29 1.2809284843093138 1103 30-31 1.2596711127751306 1103 32-33 1.3440882749580325 1103 34-35 1.4013552040097181 1103 36-37 1.5462300491471694 1103 38-39 1.6728196851177017 1103 40-41 1.5314381022789973 1103 42-43 1.5027628036195466 1103 44-45 1.533657346776124 1103 46-47 1.7616572534921104 1103 48-49 1.835271497132716 1103 50-51 1.7730354575868361 1103 52-53 1.8277082634523296 1103 54-55 1.8509477639622247 1103 56-57 1.9670472857541625 1103 58-59 2.00751970804442 1103 60-61 2.156695945056981 1103 62-63 2.2140157610634574 1103 64-65 2.2466928358795855 1103 66-67 2.4603427523327746 1103 68-69 2.560515463472708 1103 70-71 2.825646896487381 1103 72-73 2.8903683873452017 1103 74-75 2.8740357943458186 1103 76-77 3.1592108259395495 1103 78-79 3.501034866970979 1103 80-81 3.5733636743323025 1103 82-83 3.458328440410071 1103 84-85 3.6719576673027454 1103 86-87 3.7915990430070075 1103 88-89 4.112733445569315 1103 90-91 4.252218840015708 1103 92-93 4.637059057266061 1103 94-95 4.884196389868972 1103 96-97 4.810070382645375 1103 98-99 5.0280430330480606 1103 100-101 5.0904785014204865 1104 1 0.3404913625834247 1104 2 0.3608818611844491 1104 3 0.3525148875678923 1104 4 0.38299172784434177 1104 5 0.3071882763270466 1104 6 0.3940019556921257 1104 7 0.3903158656017425 1104 8 0.38500662840510813 1104 9 0.45629123363706015 1104 10-11 0.5167326379983166 1104 12-13 0.41939090652240907 1104 14-15 0.5910661798992294 1104 16-17 0.7231039786280817 1104 18-19 0.6718315175958836 1104 20-21 0.6640344944181962 1104 22-23 0.6640334291161594 1104 24-25 0.7112749220016283 1104 26-27 0.7999710764892356 1104 28-29 0.6642869596431993 1104 30-31 0.654946864027167 1104 32-33 0.7882518484686258 1104 34-35 0.7360556605763691 1104 36-37 0.8690324744230296 1104 38-39 0.8635638603026763 1104 40-41 0.8757654006696924 1104 42-43 0.931786839027076 1104 44-45 0.9841879809884801 1104 46-47 1.1311867660176134 1104 48-49 1.083573280058495 1104 50-51 1.1664973622809711 1104 52-53 1.111237741046402 1104 54-55 1.3874764924713148 1104 56-57 1.6667547674184036 1104 58-59 1.6534887510398306 1104 60-61 1.6492823970980766 1104 62-63 1.6631070912132841 1104 64-65 1.8976928125919628 1104 66-67 1.9030644342679288 1104 68-69 1.953316420334989 1104 70-71 2.148125074050281 1104 72-73 2.1170712295987677 1104 74-75 2.3480959773088586 1104 76-77 2.396345064816156 1104 78-79 2.5148350436981133 1104 80-81 2.6433775692766908 1104 82-83 2.999551079078124 1104 84-85 3.1191785490495256 1104 86-87 3.1600435790206625 1104 88-89 3.3844518969945803 1104 90-91 3.5496911309867087 1104 92-93 3.6943090744083342 1104 94-95 4.082774098703904 1104 96-97 4.176112736350628 1104 98-99 4.312906858711795 1104 100-101 4.234781994692307 1105 1 0.38287061523179133 1105 2 0.2542197923657561 1105 3 0.2202587643310494 1105 4 0.21569673538103018 1105 5 0.26822275251275585 1105 6 0.3558377545925495 1105 7 0.3909788072853999 1105 8 0.2700518473157274 1105 9 0.350020295460709 1105 10-11 0.3582663058061115 1105 12-13 0.4280539551481226 1105 14-15 0.497371082078196 1105 16-17 0.588522237151075 1105 18-19 0.5171392247756827 1105 20-21 0.4600422883151296 1105 22-23 0.40703868883309013 1105 24-25 0.494155950704922 1105 26-27 0.4559189481815551 1105 28-29 0.45822955899348017 1105 30-31 0.4043562691347873 1105 32-33 0.5526753478946773 1105 34-35 0.49975111239538705 1105 36-37 0.5384675456224244 1105 38-39 0.5245168426861966 1105 40-41 0.6904564903918455 1105 42-43 0.7346764280453115 1105 44-45 0.8545060172354013 1105 46-47 0.9182631659620668 1105 48-49 0.9630770689509305 1105 50-51 0.8333606257314727 1105 52-53 0.9356835794782583 1105 54-55 1.0038887303054835 1105 56-57 1.0821721471352035 1105 58-59 1.0878614851742228 1105 60-61 1.1701547867651954 1105 62-63 1.2389532639875753 1105 64-65 1.2233108051956378 1105 66-67 1.2996640979450866 1105 68-69 1.4840638685169871 1105 70-71 1.6609799044270694 1105 72-73 1.550776442355268 1105 74-75 1.6025424271917785 1105 76-77 1.8753334276893412 1105 78-79 1.9427178560066451 1105 80-81 2.1962274882408543 1105 82-83 2.3524624402102816 1105 84-85 2.2359231241541586 1105 86-87 2.5777406562598024 1105 88-89 2.9221266896891542 1105 90-91 3.083157927337112 1105 92-93 3.242855194776066 1105 94-95 3.39817999205248 1105 96-97 3.646342743447917 1105 98-99 3.5805475314465944 1105 100-101 3.3922443836440035 1106 1 0.23350335681386625 1106 2 0.18049980846377522 1106 3 0.18084544769195787 1106 4 0.18668005604462934 1106 5 0.3039290530985497 1106 6 0.047189541025495885 1106 7 0.13671506807330758 1106 8 0.24342751247693428 1106 9 0.2433306742113217 1106 10-11 0.17971691618933505 1106 12-13 0.3391437016043213 1106 14-15 0.48818850371214495 1106 16-17 0.2355776411666355 1106 18-19 0.23146422589359616 1106 20-21 0.3219212848719408 1106 22-23 0.3588228859443916 1106 24-25 0.41246073986558685 1106 26-27 0.25520124483227136 1106 28-29 0.2369444004727086 1106 30-31 0.2658484660672187 1106 32-33 0.2727173816110806 1106 34-35 0.35725457794115556 1106 36-37 0.4802508483993364 1106 38-39 0.21715647064308996 1106 40-41 0.4206894645008674 1106 42-43 0.38577869518299934 1106 44-45 0.4853449028947452 1106 46-47 0.6521875052725292 1106 48-49 0.6087887355430723 1106 50-51 0.5378412410335329 1106 52-53 0.6444771223839538 1106 54-55 0.28912103366370445 1106 56-57 0.6059994511641804 1106 58-59 0.699512426461169 1106 60-61 0.886136542343607 1106 62-63 0.7005952619306015 1106 64-65 0.8068885899293434 1106 66-67 1.050797666786476 1106 68-69 0.8584546032126852 1106 70-71 1.0891983903189448 1106 72-73 1.3675854572459372 1106 74-75 1.362807150171701 1106 76-77 1.4773434442345277 1106 78-79 1.6312614324498789 1106 80-81 1.8816833751969888 1106 82-83 1.9640485422538383 1106 84-85 2.1299981588096166 1106 86-87 1.8780626166407899 1106 88-89 2.279095790435921 1106 90-91 2.2862254943003997 1106 92-93 2.4916567866912835 1106 94-95 2.7261189984469674 1106 96-97 2.8061334692002795 1106 98-99 3.0097363701533872 1106 100-101 3.2945070496548237 1107 1 0.28055550947209795 1107 2 0.13924575909879522 1107 3 0.05409094877404641 1107 4 0.025142810923064474 1107 5 0.14916989148088078 1107 6 0.14613681908415543 1107 7 0.0712551912372561 1107 8 0.08185864051593228 1107 9 0.05481121692788804 1107 10-11 0.09583485719171847 1107 12-13 0.08111206572863949 1107 14-15 0.22942312516146757 1107 16-17 0.3949466405386133 1107 18-19 0.27738007139196696 1107 20-21 0.35934984210070553 1107 22-23 0.3477885075699163 1107 24-25 0.36940938239645504 1107 26-27 0.4279630811769337 1107 28-29 0.5263150715490568 1107 30-31 0.576842633174401 1107 32-33 0.4508494552925839 1107 34-35 0.5296741667018807 1107 36-37 0.5791820871322741 1107 38-39 0.6265116897493215 1107 40-41 0.5514589906856315 1107 42-43 0.6476638355383955 1107 44-45 0.5738666192381388 1107 46-47 0.7432648961486379 1107 48-49 0.8493499312183275 1107 50-51 0.7601616570620209 1107 52-53 0.6896278242738845 1107 54-55 0.7575832011865558 1107 56-57 0.7804510643588927 1107 58-59 0.9089794690354154 1107 60-61 0.9499177117234012 1107 62-63 1.0917372619215726 1107 64-65 1.1593071395224968 1107 66-67 1.2178621539120904 1107 68-69 1.376626481530682 1107 70-71 1.463984344387736 1107 72-73 1.718558208464703 1107 74-75 1.7810253392188713 1107 76-77 1.9371558970766536 1107 78-79 2.084897389455449 1107 80-81 2.079249689902113 1107 82-83 2.0895345790042263 1107 84-85 2.0829853137946834 1107 86-87 2.1922246635298386 1107 88-89 2.429486833714993 1107 90-91 2.8054336034220313 1107 92-93 3.0887177496833615 1107 94-95 3.1086223531510093 1107 96-97 3.560340742469723 1107 98-99 3.595365360218043 1107 100-101 3.6114843370659866 1108 1 0.28098901712528246 1108 2 0.19723493843344997 1108 3 0.1959861237421876 1108 4 0.1318963624770788 1108 5 0.19898332602711832 1108 6 0.20833534463425707 1108 7 0.21530939148112083 1108 8 0.1367831433900477 1108 9 0.2834576907818729 1108 10-11 0.19836923984932042 1108 12-13 0.22241918952978068 1108 14-15 0.36207810414747854 1108 16-17 0.3844396461349291 1108 18-19 0.387633444173197 1108 20-21 0.41699391791084395 1108 22-23 0.2822993838971257 1108 24-25 0.28503615457181297 1108 26-27 0.2544565543328545 1108 28-29 0.3440997557629615 1108 30-31 0.32807276025370413 1108 32-33 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-1.6698112312143394 2316 70-71 -1.7471523284178154 2316 72-73 -1.9374968538725206 2316 74-75 -1.9627501162517689 2316 76-77 -2.1597265279667397 2316 78-79 -2.7989265448564353 2316 80-81 -2.8037574259025604 2316 82-83 -2.7691993625070843 2316 84-85 -2.7936669336289235 2316 86-87 -2.637520175013389 2316 88-89 -2.909721678329255 2316 90-91 -2.8647753634300237 2316 92-93 -3.3600648746188746 2316 94-95 -3.4954565386679555 2316 96-97 -3.8209418767337695 2316 98-99 -3.769436798385474 2316 100-101 -3.6456884715455846 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6616.0 3 2105.0 4 2172.0 5 2377.0 6 2788.0 7 3376.0 8 4113.0 9 5173.0 10 6043.0 11 7590.0 12 9497.0 13 11254.0 14 12972.0 15 15853.0 16 19488.0 17 24124.0 18 23488.0 19 19415.0 20 19818.0 21 21479.0 22 23472.0 23 25881.0 24 29161.0 25 32522.0 26 37692.0 27 42395.0 28 49378.0 29 56619.0 30 66963.0 31 80278.0 32 97469.0 33 121592.0 34 158571.0 35 210256.0 36 271909.0 37 300898.0 38 155449.0 39 8486.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.03226091634945 18.558918102435733 14.504782809670191 22.90403817154462 2 15.462582145832243 19.787990309508192 40.42062891613781 24.32879862852176 3 19.268338820423246 24.210492952644667 29.50310309021098 27.01806513672111 4 12.753596792922533 16.541474056200332 36.63446038546914 34.070468765407995 5 13.8708333249458 37.3241889087282 33.69760391650848 15.10737384981752 6 32.32939225062428 35.633248821650824 17.258248444216754 14.779110483508143 7 28.97936391351156 32.89971727253519 19.98576301200334 18.135155801949914 8 26.808107565642697 34.66621661524779 19.28336186397749 19.24231395513202 9 26.921325840220828 16.674271461493998 19.9560716195597 36.448331078725474 10-11 25.45908466053657 26.51259663963838 26.49659851111757 21.531720188707485 12-13 26.240090631561024 23.890114262875294 27.355654675441066 22.51414043012262 14-15 22.953746321587566 25.24213385648532 25.99685605774793 25.80726376417918 16-17 22.29516245298499 28.566840163808667 26.551250069796257 22.586747313410086 18-19 22.15538918985134 26.826582995835324 29.169663085553072 21.848364728760263 20-21 23.01746841002779 25.91812857258933 29.298383264578455 21.76601975280443 22-23 22.660111284867074 25.79015852597481 29.299497957899295 22.250232231258817 24-25 22.400122771001893 26.01321297749869 29.39328986032283 22.19337439117659 26-27 22.13974446149175 26.34268905842278 29.30806475024106 22.209501729844412 28-29 22.024959667419072 26.58537100692357 29.386718394323104 22.002950931334254 30-31 22.17370131748468 26.205413715544246 29.31510315660053 22.305781810370544 32-33 22.040996607846875 26.271831043307177 29.219829934556547 22.4673424142894 34-35 22.614898053281397 26.354692627945603 28.980804480690153 22.04960483808285 36-37 22.580092608080278 26.136491401846072 29.022891435651623 22.260524554422023 38-39 22.199091444976695 26.398819642975695 29.004552834405988 22.397536077641618 40-41 22.365707077641762 26.21935302806945 29.071161681622055 22.343778212666738 42-43 22.372456575183175 26.18528022435652 28.768966294214447 22.673296906245852 44-45 22.28755579784634 26.471639908510642 28.736846393231236 22.503957900411784 46-47 22.41444869788798 26.735860150397873 28.359369538067984 22.49032161364616 48-49 22.50495806954425 26.53141854355733 28.352110203689517 22.611513183208906 50-51 22.468024114714627 26.828009781672563 28.135183217767946 22.56878288584486 52-53 22.64494726093034 26.985701975159692 27.839444504095333 22.529906259814634 54-55 22.34000527559449 26.82624711357339 27.98702877663289 22.84671883419923 56-57 22.46490040047012 26.94696725479063 27.969340373453605 22.618791971285642 58-59 22.614074548108512 26.630750874740215 28.00722674347701 22.74794783367426 60-61 22.4697342772135 26.76783907366168 28.217455546905335 22.544971102219485 62-63 22.32687330281811 26.84888628155259 28.21337085751832 22.61086955811098 64-65 22.166263826568535 26.686698028849587 28.21379392893317 22.933244215648703 66-67 22.234422628151826 26.80476397494757 28.007474795070475 22.95333860183013 68-69 22.2411590334247 26.66686614856691 28.144222396503466 22.94775242150492 70-71 22.34112508043671 26.6797410635726 28.002939098942875 22.976194757047814 72-73 22.35431295218381 26.670383211476935 27.894102109931275 23.081201726407986 74-75 22.19962242213085 27.01042110895646 27.71287285060227 23.077083618310414 76-77 22.33765032075048 26.744050093045672 27.702014468407594 23.21628511779625 78-79 22.397071081836458 26.78086798871984 27.748431879644798 23.073629049798903 80-81 22.4060372720225 27.108329060595267 27.5096102030722 22.976023464310042 82-83 22.456615647058204 26.924940599778164 27.52333072740975 23.095113025753886 84-85 22.5546767622566 26.649003072228293 27.544244060350252 23.252076105164853 86-87 22.490232771675274 26.66074309631258 27.57087604727894 23.278148084733203 88-89 22.50052246787923 26.627301970841895 27.470594049122294 23.40158151215659 90-91 22.532400183893618 26.653357517154518 27.53520184537444 23.279040453577423 92-93 22.70900415537716 26.28210044571292 27.523332348448175 23.485563050461742 94-95 22.490756152044263 26.309266382271527 27.61756449316149 23.58241297252272 96-97 22.564036662362934 26.1512314926539 27.587985496407047 23.696746348576124 98-99 22.627467577814876 26.148384479800306 27.200082685342288 24.024065257042526 100-101 23.316275876065315 25.32804529496442 27.194428070751474 24.161250758218788 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1212.0 1 808.0 2 572.0 3 757.0 4 626.5 5 588.5 6 1094.5 7 2331.0 8 3788.5 9 4362.0 10 3579.0 11 2663.5 12 2487.0 13 2630.5 14 2885.5 15 3047.5 16 3094.0 17 3166.0 18 3343.5 19 3526.5 20 3715.5 21 4229.5 22 5015.5 23 6006.5 24 7189.5 25 8736.5 26 10826.0 27 13289.5 28 15569.0 29 17982.5 30 21122.5 31 24248.0 32 27712.0 33 30686.0 34 33544.5 35 36699.0 36 40517.0 37 44622.5 38 47655.0 39 50138.0 40 52407.0 41 55810.0 42 60108.5 43 64253.5 44 67972.0 45 71201.0 46 72211.5 47 74072.0 48 78732.5 49 79572.5 50 76614.5 51 73564.0 52 71335.5 53 70763.5 54 70348.0 55 66668.5 56 60494.0 57 55241.0 58 50527.5 59 44232.5 60 36955.5 61 30639.0 62 25110.5 63 19802.5 64 15520.5 65 12180.0 66 9203.0 67 7226.0 68 5546.5 69 3880.5 70 2842.5 71 2162.0 72 1673.0 73 1330.5 74 1026.5 75 799.5 76 525.5 77 287.5 78 171.0 79 95.5 80 49.5 81 27.5 82 17.5 83 14.5 84 10.5 85 7.5 86 9.5 87 9.0 88 6.5 89 4.0 90 0.5 91 0.0 92 1.0 93 1.5 94 1.0 95 1.0 96 1.5 97 1.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4649696389458208 2 0.040427770056498315 3 0.05209349474941822 4 0.056719557989714044 5 0.08326913832532488 6 0.08196177262698041 7 0.04786969787784377 8 0.040779753129129515 9 0.0478194145817536 10-11 0.050258154442126945 12-13 0.04543095801747043 14-15 0.03932153754251453 16-17 0.040377486760408136 18-19 0.047392006564987134 20-21 0.056091016788586905 22-23 0.04779427293370852 24-25 0.06496601854850227 26-27 0.05659384974948862 28-29 0.04450071703980225 30-31 0.046713182067769816 32-33 0.045707516145966375 34-35 0.05040900433039745 36-37 0.044877841760478536 38-39 0.026147313966889452 40-41 0.024387398603733432 42-43 0.02353258257020051 44-45 0.015286122011412296 46-47 0.027102696592602726 48-49 0.02810836251440616 50-51 0.016065513100809965 52-53 0.015612963435998417 54-55 0.016618629357801857 56-57 0.016040371452764875 58-59 0.014305597737653943 60-61 0.017297453855019178 62-63 0.016367212877350996 64-65 0.01759915363156021 66-67 0.009176701536456396 68-69 0.012445115782317578 70-71 0.01847911131313822 72-73 0.016392354525396082 74-75 0.013475923352166104 76-77 0.009679534497358115 78-79 0.014305597737653943 80-81 0.017624295279605295 82-83 0.01576381332426893 84-85 0.013727339832616965 86-87 0.00334383918999644 88-89 0.028736903715533318 90-91 0.031477343352447694 92-93 0.017322595503064264 94-95 0.032834992346882336 96-97 0.026901563408242037 98-99 0.02370857410651611 100-101 0.1504727635498398 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1988732.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.66207157146752 #Duplication Level Percentage of deduplicated Percentage of total 1 75.41861940755331 41.22537971876949 2 13.632338764744626 14.903437544899239 3 4.657290181352097 7.637313876664839 4 2.146033280464021 4.692264990859022 5 1.0985562048185522 3.002467894653572 6 0.6807984493829399 2.2328312139548623 7 0.44566879446217195 1.7052825678042622 8 0.3297505805147742 1.4419879866265242 9 0.2388750305203512 1.1751663613445933 >10 1.2244051856863793 11.937966508814128 >50 0.08016019691024177 2.9946044109275474 >100 0.04370864768954612 4.282597581763363 >500 0.0028695988519522393 1.0479646933584352 >1k 6.479739343117939E-4 0.4987079338390572 >5k 1.851354098033697E-4 0.6987780607059031 >10k+ 9.256770490168484E-5 0.5232486550151878 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10341 0.519979564868469 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8384 0.4215751544200023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5426 0.27283716458527346 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.028329609017203E-5 0.0 18-19 0.0 0.0 0.0 1.2570824022543008E-4 0.0 20-21 0.0 0.0 0.0 2.011331843606881E-4 0.0 22-23 0.0 0.0 0.0 2.5141648045086016E-4 0.0 24-25 5.028329609017203E-5 0.0 0.0 7.542494413525805E-4 0.0 26-27 5.028329609017203E-5 0.0 0.0 0.001583923826840419 0.0 28-29 1.0056659218034405E-4 0.0 0.0 0.004877479720746687 0.0 30-31 1.0056659218034405E-4 0.0 0.0 0.016266646285170654 0.0 32-33 1.0056659218034405E-4 0.0 0.0 0.0391958293022891 0.0 34-35 1.7599153631560208E-4 0.0 0.0 0.07275992944247892 0.0 36-37 2.011331843606881E-4 0.0 0.0 0.12734244734836067 0.0 38-39 2.011331843606881E-4 0.0 0.0 0.20862539547812375 0.0 40-41 2.011331843606881E-4 0.0 0.0 0.29926103668065884 0.0 42-43 2.5141648045086016E-4 0.0 0.0 0.3865025553971073 0.0 44-45 4.022663687213762E-4 0.0 0.0 0.4897593039182756 0.0 46-47 4.022663687213762E-4 0.0 0.0 0.6170514679705461 0.0 48-49 4.022663687213762E-4 0.0 0.0 0.7472349215480014 0.0 50-51 4.022663687213762E-4 0.0 0.0 0.8861676686451467 0.0 52-53 4.022663687213762E-4 0.0 0.0 1.0178847627533523 2.5141648045086013E-5 54-55 4.022663687213762E-4 0.0 0.0 1.1610413067220722 5.028329609017203E-5 56-57 4.5254966481154827E-4 0.0 0.0 1.3233306448531024 5.028329609017203E-5 58-59 4.5254966481154827E-4 0.0 0.0 1.4945704096881833 5.028329609017203E-5 60-61 4.5254966481154827E-4 0.0 0.0 1.6750371593558104 5.028329609017203E-5 62-63 4.5254966481154827E-4 0.0 0.0 1.8529897442189294 5.028329609017203E-5 64-65 4.5254966481154827E-4 0.0 0.0 2.0365489165961024 5.028329609017203E-5 66-67 4.5254966481154827E-4 0.0 0.0 2.238788333470774 5.028329609017203E-5 68-69 5.028329609017203E-4 0.0 0.0 2.459029170345728 5.028329609017203E-5 70-71 5.028329609017203E-4 0.0 0.0 2.6963914695393845 5.028329609017203E-5 72-73 5.028329609017203E-4 0.0 0.0 2.9251050418055327 5.028329609017203E-5 74-75 5.028329609017203E-4 0.0 0.0 3.1557293793231063 5.028329609017203E-5 76-77 5.028329609017203E-4 0.0 0.0 3.4062910437404335 5.028329609017203E-5 78-79 5.028329609017203E-4 0.0 0.0 3.6831005887168304 5.028329609017203E-5 80-81 5.028329609017203E-4 0.0 0.0 3.978942361263358 5.028329609017203E-5 82-83 6.033995530820643E-4 0.0 0.0 4.273401343167405 5.028329609017203E-5 84-85 6.033995530820643E-4 0.0 0.0 4.570701331300548 5.028329609017203E-5 86-87 6.033995530820643E-4 0.0 0.0 4.872350824545489 5.028329609017203E-5 88-89 6.033995530820643E-4 0.0 0.0 5.1978597417852175 5.028329609017203E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6155 0.0 41.344173 1 GTATCAA 9750 0.0 36.040302 1 TATCAAC 11940 0.0 29.138058 2 ATCAACG 11965 0.0 29.117632 3 TCAACGC 12155 0.0 28.662483 4 CAACGCA 12220 0.0 28.43223 5 AACGCAG 12590 0.0 27.668674 6 GTACATG 8995 0.0 25.47741 1 ACGCAGA 14195 0.0 24.4665 7 TACATGG 9010 0.0 24.37087 2 CGCAGAG 14360 0.0 23.953777 8 GAGTACT 7665 0.0 23.210865 12-13 ACATGGG 9320 0.0 22.336903 3 GCAGAGT 15275 0.0 22.049578 9 GTATAGA 590 0.0 21.848766 1 GTACTTT 8280 0.0 21.487679 14-15 AGTACTT 8080 0.0 20.460655 12-13 GGTATAG 355 3.6379788E-12 20.173353 1 AGAGTAC 12560 0.0 20.121357 10-11 CAGAGTA 14660 0.0 19.410114 10-11 >>END_MODULE