FastQCFastQC Report
Thu 26 May 2016
SRR938066_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938066_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988732
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105290.5294328245334213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85380.4293187820178888No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57490.289078669222399No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21980.11052268480619813No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63150.039.795871
GTATCAA100000.036.3426971
ACCGTAC753.081212E-431.663238
TATCAAC123850.029.152332
ATCAACG124400.028.866333
TCAACGC126250.028.5909584
CAACGCA128300.028.1711435
AACGCAG130650.027.7007836
ACGCAGA149900.024.0801097
CGCAGAG151300.023.7003368
GTACATG90950.023.6619871
GAGTACT81100.022.98610112-13
TACATGG92550.022.9449312
GCAGAGT160100.022.2493049
GGACCGA5700.021.6643166
GTACTTT85550.021.65165514-15
TAAGGTG7500.021.5309965
ACATGGG94300.021.5083123
AGTACTT84000.021.0334312-13
AGAGTAC134950.020.53593410-11
Produced by FastQC (version 0.11.5)