##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938066_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1988732 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.476537311211366 33.0 31.0 34.0 31.0 34.0 2 32.704987399006 34.0 31.0 34.0 31.0 34.0 3 32.67003196006299 34.0 31.0 34.0 31.0 34.0 4 36.20213030212216 37.0 35.0 37.0 35.0 37.0 5 36.16505542224895 37.0 35.0 37.0 35.0 37.0 6 36.22411918750239 37.0 37.0 37.0 35.0 37.0 7 36.182942196334146 37.0 37.0 37.0 35.0 37.0 8 36.177358739136295 37.0 37.0 37.0 35.0 37.0 9 37.872587658870074 39.0 38.0 39.0 35.0 39.0 10-11 37.83899917133128 39.0 38.0 39.0 35.0 39.0 12-13 37.78292826786113 39.0 38.0 39.0 35.0 39.0 14-15 39.04999668130246 40.0 38.0 41.0 36.0 41.0 16-17 38.990467795560185 40.0 38.0 41.0 36.0 41.0 18-19 38.992299616036746 40.0 38.5 41.0 35.5 41.0 20-21 38.99690028621252 40.0 38.5 41.0 35.5 41.0 22-23 39.0210350112534 40.0 39.0 41.0 35.0 41.0 24-25 38.97492824573648 40.0 38.0 41.0 35.0 41.0 26-27 38.81014485611938 40.0 38.0 41.0 35.0 41.0 28-29 38.73441544662629 40.0 38.0 41.0 35.0 41.0 30-31 38.52271975308891 40.0 38.0 41.0 34.0 41.0 32-33 38.333349088766106 40.0 38.0 41.0 34.0 41.0 34-35 38.131404080590045 40.0 38.0 41.0 34.0 41.0 36-37 38.0679639086614 40.0 38.0 41.0 34.0 41.0 38-39 38.029146209745704 40.0 38.0 41.0 33.0 41.0 40-41 37.90984205011033 40.0 37.5 41.0 33.0 41.0 42-43 37.71933121204868 40.0 37.0 41.0 33.0 41.0 44-45 37.46818802131207 40.0 37.0 41.0 33.0 41.0 46-47 37.10130123113622 40.0 37.0 41.0 31.5 41.0 48-49 36.72861929108598 40.0 36.0 41.0 31.0 41.0 50-51 36.43455855288697 39.0 36.0 41.0 30.0 41.0 52-53 35.98629855606487 39.0 35.0 40.5 29.0 41.0 54-55 35.682005167111505 39.0 35.0 40.0 28.0 41.0 56-57 35.41642237365316 38.0 35.0 40.0 28.0 41.0 58-59 35.05171536436282 38.0 34.0 40.0 27.5 41.0 60-61 34.64336547106397 38.0 34.0 40.0 26.0 41.0 62-63 34.22267907390237 37.0 34.0 40.0 26.0 41.0 64-65 33.78806470655674 36.5 33.0 39.0 25.0 41.0 66-67 33.28241361832565 36.0 32.5 39.0 23.5 40.0 68-69 32.76369515852312 35.0 32.0 38.5 22.0 40.0 70-71 32.182378269168495 35.0 31.0 38.0 20.5 40.0 72-73 31.718426112719058 35.0 31.0 37.0 19.5 39.0 74-75 31.16415660833134 34.5 30.5 36.5 18.0 39.0 76-77 29.95163149182494 33.5 29.5 35.0 17.5 37.5 78-79 30.647239799027723 34.0 30.0 36.0 18.0 38.0 80-81 30.46648090340981 34.0 30.0 35.5 14.5 37.0 82-83 30.019504387720417 34.0 30.0 35.0 8.5 37.0 84-85 29.517117439655017 34.0 29.5 35.0 6.0 36.0 86-87 28.976464149015555 34.0 29.0 35.0 2.0 36.0 88-89 28.37981739118192 33.5 28.5 35.0 2.0 35.5 90-91 27.866079743273602 33.0 27.0 35.0 2.0 35.0 92-93 27.24426267591611 33.0 26.0 35.0 2.0 35.0 94-95 26.513554868127027 32.5 25.0 34.5 2.0 35.0 96-97 25.692971199739333 32.0 23.5 34.0 2.0 35.0 98-99 24.699882638786924 32.0 10.5 34.0 2.0 35.0 100-101 21.203408000675807 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.008721781501449755 1101 2 0.056480722460982236 1101 3 0.12791587670020022 1101 4 0.09387525219435844 1101 5 0.028576870070018856 1101 6 0.037525079471784295 1101 7 9.79930832613718E-4 1101 8 0.07103733344001739 1101 9 0.059831195518256663 1101 10-11 0.06965834117586667 1101 12-13 0.13163481531294252 1101 14-15 0.17429403684700873 1101 16-17 0.24776274985185864 1101 18-19 0.2401144356300975 1101 20-21 0.15976132374791518 1101 22-23 0.14536054654800523 1101 24-25 0.16447253720136956 1101 26-27 0.13750520920149256 1101 28-29 0.1877555561828217 1101 30-31 0.21100160660788703 1101 32-33 0.27588152015606937 1101 34-35 0.18671134317686722 1101 36-37 0.09086671981090433 1101 38-39 0.18937056831152432 1101 40-41 0.14094439914516244 1101 42-43 0.24026583681354197 1101 44-45 0.3643995946263203 1101 46-47 0.33834879090571945 1101 48-49 0.398765127032803 1101 50-51 0.44972138841511367 1101 52-53 0.43056364648222 1101 54-55 0.5120719666906126 1101 56-57 0.46613800771954317 1101 58-59 0.49829596629109574 1101 60-61 0.6179560825730093 1101 62-63 0.6178218531270474 1101 64-65 0.6894504643227677 1101 66-67 0.5772532919712461 1101 68-69 0.7291536157084906 1101 70-71 0.6494007558464574 1101 72-73 0.7793415387511651 1101 74-75 0.6967974255419271 1101 76-77 0.8271509984082677 1101 78-79 0.9109341329347664 1101 80-81 1.1294181673399777 1101 82-83 1.1876625768790419 1101 84-85 1.3807424619851645 1101 86-87 1.4207697739585363 1101 88-89 1.6995694611597152 1101 90-91 1.811313338008219 1101 92-93 1.8499104947406728 1101 94-95 2.1114068062860483 1101 96-97 2.2865177560476333 1101 98-99 2.5850617199488894 1101 100-101 3.0242214374904037 1102 1 0.06608412625432436 1102 2 0.09753223263023614 1102 3 0.2348236847138523 1102 4 0.07528813884417929 1102 5 -0.024360542150340336 1102 6 0.06443538006760718 1102 7 0.12271800358725926 1102 8 0.13676301039178185 1102 9 0.20532129948769295 1102 10-11 0.1735420957715661 1102 12-13 0.16779553173858375 1102 14-15 0.2518169374457102 1102 16-17 0.25483035874654547 1102 18-19 0.18445106124905664 1102 20-21 0.26445936179814566 1102 22-23 0.26768191948143993 1102 24-25 0.16631950463960976 1102 26-27 0.2200695969130635 1102 28-29 0.09659056162335844 1102 30-31 0.20743559632119712 1102 32-33 0.13491760986985213 1102 34-35 0.09282997331123255 1102 36-37 0.2187424765732331 1102 38-39 0.22080945930419205 1102 40-41 0.2158944276660506 1102 42-43 0.24796917399759622 1102 44-45 0.23415019260637848 1102 46-47 0.33964421462071215 1102 48-49 0.3965631463886581 1102 50-51 0.321088869325294 1102 52-53 0.2902585328540326 1102 54-55 0.4107368174472583 1102 56-57 0.45134848711020226 1102 58-59 0.425269300455291 1102 60-61 0.4970607542472081 1102 62-63 0.5290137268298238 1102 64-65 0.716713081821652 1102 66-67 0.6472295405392146 1102 68-69 0.7834298920783311 1102 70-71 0.9842453169842287 1102 72-73 1.0355604114329502 1102 74-75 0.967976884268122 1102 76-77 1.012529307670608 1102 78-79 1.2215946552265962 1102 80-81 1.0922334550175137 1102 82-83 1.2879911063993603 1102 84-85 1.2381892309755251 1102 86-87 1.2526079574413416 1102 88-89 1.319964775105234 1102 90-91 1.4948869219099734 1102 92-93 1.610883548489376 1102 94-95 1.5422583226867594 1102 96-97 1.9048114517321046 1102 98-99 2.1902657641634633 1102 100-101 2.8559730396934633 1103 1 0.2842128517962479 1103 2 0.37480376079000166 1103 3 0.4156113947754818 1103 4 0.21352328438587875 1103 5 0.14278228291897932 1103 6 0.16723507921781788 1103 7 0.28347198030507315 1103 8 0.2676368779954217 1103 9 0.2890681141844311 1103 10-11 0.29222902115408544 1103 12-13 0.27183076371846226 1103 14-15 0.438057549921993 1103 16-17 0.4954407302062265 1103 18-19 0.41128604850784 1103 20-21 0.433243951104501 1103 22-23 0.48741241756744813 1103 24-25 0.5446107508088289 1103 26-27 0.5758974389893723 1103 28-29 0.49179127782546317 1103 30-31 0.5293666197717712 1103 32-33 0.6384396852843608 1103 34-35 0.6445209904753781 1103 36-37 0.5619855643248357 1103 38-39 0.6205692285030082 1103 40-41 0.6260955367690073 1103 42-43 0.5696406022482918 1103 44-45 0.6775163425148847 1103 46-47 0.8184724720493435 1103 48-49 0.9095653578591225 1103 50-51 0.9953621430873696 1103 52-53 0.8715897089746321 1103 54-55 0.8951590006288583 1103 56-57 1.1161451328595788 1103 58-59 1.1469958751457412 1103 60-61 1.1390193368854753 1103 62-63 1.11128365532187 1103 64-65 1.1640391109412178 1103 66-67 1.0458737519872159 1103 68-69 1.2045725880516542 1103 70-71 1.3057778267225615 1103 72-73 1.3203742325620276 1103 74-75 1.1999462436152264 1103 76-77 0.9870433735207911 1103 78-79 1.2513197088294987 1103 80-81 1.4834385099675025 1103 82-83 1.472669236415861 1103 84-85 1.4665972248238184 1103 86-87 1.6173167617879471 1103 88-89 1.7621606417883662 1103 90-91 1.7396802841308734 1103 92-93 1.9735415507569734 1103 94-95 2.0576719630814573 1103 96-97 2.116084759171404 1103 98-99 2.616604573786166 1103 100-101 2.6571560921962423 1104 1 0.0791540699714588 1104 2 0.09442991692311864 1104 3 0.19264120163057896 1104 4 0.05796621226024712 1104 5 -0.06135372711022313 1104 6 0.0075525411781427465 1104 7 0.04748142213972528 1104 8 -0.005834451714534339 1104 9 0.05624531871309557 1104 10-11 0.13027642568456343 1104 12-13 -0.03478887058833635 1104 14-15 0.18265449950300905 1104 16-17 0.12365086342433074 1104 18-19 0.17250372561986893 1104 20-21 0.1618057596725322 1104 22-23 0.11668941470695415 1104 24-25 0.05115101315676185 1104 26-27 0.23325574354741008 1104 28-29 0.07276539234619861 1104 30-31 0.07648054020315698 1104 32-33 0.21873150499164495 1104 34-35 0.25837915983586157 1104 36-37 0.1711024396959786 1104 38-39 0.24238254462076014 1104 40-41 0.17420532623601304 1104 42-43 0.2208176618762323 1104 44-45 0.17507955222116323 1104 46-47 0.2906175720114348 1104 48-49 0.19402860417871182 1104 50-51 0.30198420318752994 1104 52-53 0.312600397990515 1104 54-55 0.2657665357252341 1104 56-57 0.3128629050627012 1104 58-59 0.4029511978538878 1104 60-61 0.4072494974535914 1104 62-63 0.3994061300709788 1104 64-65 0.36513964487451034 1104 66-67 0.3571939269548423 1104 68-69 0.3571925095723927 1104 70-71 0.4781281811471416 1104 72-73 0.4840391147072829 1104 74-75 0.5249115062542629 1104 76-77 0.6335755240157184 1104 78-79 0.732346446894276 1104 80-81 0.8586484219532515 1104 82-83 0.7345829545037432 1104 84-85 0.9715330674409337 1104 86-87 0.9264590594735473 1104 88-89 1.124360377085793 1104 90-91 1.1777261684975073 1104 92-93 1.409645506676103 1104 94-95 1.471512310946693 1104 96-97 1.7988639227061007 1104 98-99 2.083732001035006 1104 100-101 2.3703147597823353 1105 1 0.04677043319418317 1105 2 0.08725019412582213 1105 3 0.16635301409779402 1105 4 0.08728962308778421 1105 5 0.05375824888920988 1105 6 0.07098452442586733 1105 7 0.07737420035800824 1105 8 0.042674907323842604 1105 9 0.07279187774126683 1105 10-11 0.10443321114519222 1105 12-13 0.07371534929045964 1105 14-15 0.1183241535331021 1105 16-17 0.14109764180747675 1105 18-19 0.09514204538204041 1105 20-21 0.1515338768414125 1105 22-23 0.20771034242427788 1105 24-25 0.16023982481522836 1105 26-27 0.1962572602926329 1105 28-29 0.12860597541801155 1105 30-31 0.11093036173123494 1105 32-33 0.22237038149609845 1105 34-35 0.14015299176009677 1105 36-37 0.127001964670292 1105 38-39 0.14671273215800085 1105 40-41 0.28748072107373446 1105 42-43 0.19273708104227438 1105 44-45 0.18470619326794946 1105 46-47 0.2448292939987553 1105 48-49 0.26577545551515414 1105 50-51 0.2624780279030716 1105 52-53 0.32123151253212967 1105 54-55 0.4565970719381198 1105 56-57 0.5156255251528066 1105 58-59 0.40062731247100203 1105 60-61 0.40109277932683796 1105 62-63 0.41418393692224953 1105 64-65 0.4149014883969997 1105 66-67 0.5574529396074226 1105 68-69 0.6259122572881708 1105 70-71 0.6151083208698935 1105 72-73 0.5674584926560833 1105 74-75 0.4227916276829973 1105 76-77 0.35916556810703426 1105 78-79 0.6424366520175404 1105 80-81 0.8046850184136183 1105 82-83 0.7376475125966664 1105 84-85 0.8730082488401472 1105 86-87 0.9845038370006662 1105 88-89 1.1482929927026468 1105 90-91 1.0780489998080611 1105 92-93 1.1942614968394167 1105 94-95 1.216965850443831 1105 96-97 1.3608003263489614 1105 98-99 1.5150338645249661 1105 100-101 1.8940810211006784 1106 1 0.025158842620470523 1106 2 0.04505996920072164 1106 3 0.09428754882032564 1106 4 0.06479264146635444 1106 5 0.029486013500395813 1106 6 -0.010498281995452885 1106 7 0.07994988340589515 1106 8 -0.025339223159548396 1106 9 -0.07107075249617623 1106 10-11 0.07101864243796285 1106 12-13 0.0048426577911087065 1106 14-15 0.009503780595665035 1106 16-17 0.10809893859234876 1106 18-19 0.03875649782580837 1106 20-21 0.03781031560186676 1106 22-23 0.06827243158941343 1106 24-25 0.08656902930479049 1106 26-27 0.05421012887018861 1106 28-29 0.04183094523422426 1106 30-31 -0.018368032938532508 1106 32-33 0.04339841887928486 1106 34-35 -0.06474796070603617 1106 36-37 -0.050954928412039635 1106 38-39 0.1095017139582879 1106 40-41 0.11054560503384181 1106 42-43 0.1395554864440598 1106 44-45 0.3610823503130689 1106 46-47 0.02327807099647572 1106 48-49 0.01832878020324813 1106 50-51 -0.03345945812294815 1106 52-53 0.005135729318787696 1106 54-55 0.007520472197469985 1106 56-57 0.21483180338022834 1106 58-59 0.18249021100876917 1106 60-61 0.1919415026644984 1106 62-63 0.174728139399555 1106 64-65 0.22247873653007844 1106 66-67 0.3253709291437161 1106 68-69 0.09481625049122755 1106 70-71 0.155125313223337 1106 72-73 0.37318446859849175 1106 74-75 0.3141948383434645 1106 76-77 0.2839118007234056 1106 78-79 0.6158078455067866 1106 80-81 0.7501932239316638 1106 82-83 0.5723946395474364 1106 84-85 0.6972871917368977 1106 86-87 0.8862522607363026 1106 88-89 1.0098187271747676 1106 90-91 0.7742905595272411 1106 92-93 0.8046715491579768 1106 94-95 1.056801740082971 1106 96-97 1.1093582121424639 1106 98-99 1.4050933377620076 1106 100-101 1.9123589802295982 1107 1 0.032611933240403346 1107 2 0.035852040803888485 1107 3 0.1033224332242284 1107 4 0.0450900241194887 1107 5 -0.00713967381859959 1107 6 0.066359004150641 1107 7 0.02199731462881971 1107 8 -0.0015219540015891653 1107 9 -0.02944124736585252 1107 10-11 0.04256917083208123 1107 12-13 -0.02176726128936224 1107 14-15 0.019029558728867357 1107 16-17 -0.03153062075541868 1107 18-19 -0.029134353433164506 1107 20-21 0.03128231003554305 1107 22-23 -0.001218511839205405 1107 24-25 0.022194856685494813 1107 26-27 0.10050594825382575 1107 28-29 0.024808250717867963 1107 30-31 6.735834640494431E-4 1107 32-33 0.03302120104716266 1107 34-35 0.01989178700215888 1107 36-37 0.0038715531924324864 1107 38-39 0.030184085609796796 1107 40-41 0.02268669688270819 1107 42-43 -0.052071081908323436 1107 44-45 0.03292633059599126 1107 46-47 0.17878656280286265 1107 48-49 0.08624990286568845 1107 50-51 0.01488600636237436 1107 52-53 -0.028290677284893206 1107 54-55 0.05833192653509656 1107 56-57 0.11618791707420684 1107 58-59 0.13062287204586198 1107 60-61 0.1572784866387238 1107 62-63 0.2998728321899975 1107 64-65 0.2250384773707239 1107 66-67 0.40393696824352077 1107 68-69 0.42913024175319237 1107 70-71 0.4416495687501225 1107 72-73 0.3354473237791886 1107 74-75 0.4253161905263916 1107 76-77 0.5585233553632918 1107 78-79 0.712043799801485 1107 80-81 0.7239777367201974 1107 82-83 0.8859322822635463 1107 84-85 0.9779744881391181 1107 86-87 1.0498800399448136 1107 88-89 1.0623176993024863 1107 90-91 1.1278296028605261 1107 92-93 1.3829312597272185 1107 94-95 1.4693252907830505 1107 96-97 1.7189237497309584 1107 98-99 1.5471111752994524 1107 100-101 2.130907217936926 1108 1 0.11482187929423304 1108 2 0.12131525879274818 1108 3 0.18998663767220592 1108 4 0.1539495584720214 1108 5 0.10459921574696551 1108 6 0.09990084025854884 1108 7 0.07893808648199041 1108 8 0.08382601119730992 1108 9 0.11824145122583474 1108 10-11 0.11371419420351714 1108 12-13 0.10063775011149545 1108 14-15 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>>Per sequence quality scores pass #Quality Count 2 2.0 3 4.0 4 13.0 5 38.0 6 83.0 7 189.0 8 405.0 9 749.0 10 1317.0 11 2160.0 12 3450.0 13 5149.0 14 7023.0 15 9668.0 16 12963.0 17 18420.0 18 18523.0 19 13367.0 20 11665.0 21 12456.0 22 12762.0 23 14051.0 24 16241.0 25 18755.0 26 22051.0 27 26885.0 28 32761.0 29 39433.0 30 48650.0 31 59536.0 32 75814.0 33 99221.0 34 137094.0 35 201877.0 36 308934.0 37 438906.0 38 297857.0 39 20260.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.305231675258405 18.997029262866995 14.944044748110857 22.753694313763745 2 15.714805127666134 20.113733569959773 39.90772976911824 24.263731533255857 3 19.569738801954735 24.14254005066866 29.631611289516375 26.656109857860226 4 13.009646347521938 16.846462972386426 36.46514462481621 33.67874605527542 5 13.859144787886498 37.38615181702027 33.790829592658206 14.96387380243503 6 32.380061647383 35.84785264968095 17.071950440733534 14.70013526220251 7 29.000689886822357 32.9246977471072 19.962921097463106 18.111691268607334 8 26.859352451061735 34.91595138606045 19.115013098811804 19.10968306406601 9 26.642151883712838 16.718391417244757 20.016774507575683 36.62268219146672 10-11 25.431053555733 26.64813559594757 26.418164941279166 21.502645907040264 12-13 26.160588757057262 23.849166202384232 27.47031777031797 22.519927270240537 14-15 22.87711466401707 25.268814500898056 25.99910395166367 25.85496688342119 16-17 22.248478489931898 28.642680019902134 26.596526479316086 22.512315010849882 18-19 22.107377967468718 26.900482317376095 29.202049346015453 21.790090369139733 20-21 22.9033374029281 25.936752664511857 29.409342234147186 21.75056769841286 22-23 22.67681115404187 25.753947741576038 29.408462276465606 22.16077882791648 24-25 22.443944508444968 26.0223895119372 29.429793045604185 22.103872934013644 26-27 22.12637897093838 26.402961788661226 29.404181952241608 22.06647728815878 28-29 21.960628040578765 26.584344240656705 29.52182931275063 21.933198406013904 30-31 22.11341171678875 26.178699696162493 29.410489873549793 22.297398713498957 32-33 21.983082548444138 26.26212722006038 29.38682472312337 22.36796550837211 34-35 22.60674518955129 26.31896317605287 29.116391233064103 21.957900401331738 36-37 22.556814453097008 26.12226507016522 29.144859600418695 22.176060876319077 38-39 22.18562706350717 26.40348664656713 29.179868041160372 22.231018248765324 40-41 22.38105093687342 26.15874729125398 29.181603487112064 22.27859828476053 42-43 22.33471481196392 26.203844083107995 28.92542655803606 22.536014546892027 44-45 22.272854365494844 26.437823795112557 28.86391729351288 22.42540454587971 46-47 22.405557258249637 26.699011428946406 28.4034347432227 22.491996569581257 48-49 22.444894314143138 26.553910949935737 28.46898312686929 22.532211609051835 50-51 22.430448144848075 26.823297457877683 28.235151845497537 22.51110255177671 52-53 22.645585227169875 26.9306522950302 27.927795198146356 22.495967279653566 54-55 22.372722920936557 26.780305239720587 28.07067518398658 22.776296655356276 56-57 22.4213720099038 26.96253190475137 28.09642023158475 22.519675853760084 58-59 22.572699589487172 26.65497412421583 28.05767695194727 22.714649334349726 60-61 22.43967512968062 26.867597041733127 28.20744574935185 22.485282079234405 62-63 22.248724312778194 26.897591027850915 28.245837045916694 22.6078476134542 64-65 22.081959761295135 26.73965119483168 28.191556227787355 22.98683281608583 66-67 22.115573903413114 26.957187447752396 27.968864512609194 22.9583741362253 68-69 22.132066861850102 26.758291420016068 28.18412779025784 22.92551392787599 70-71 22.237863120822716 26.8047932049165 27.900667359905707 23.056676314355077 72-73 22.324626948226307 26.776508850865778 27.804978247446112 23.093885953461804 74-75 22.17593924168767 27.060508907183067 27.58707558383935 23.17647626728991 76-77 22.19730964252599 26.873656178911993 27.71197929132734 23.21705488723468 78-79 22.22576998811303 26.90103543363309 27.750119171411736 23.123075406842148 80-81 22.192381811987815 27.199804900369706 27.604133799125318 23.003679488517154 82-83 22.225619176657023 27.032580676061535 27.642926229295934 23.098873917985507 84-85 22.324204386168923 26.813660106479944 27.600203657026483 23.261931850324657 86-87 22.307975443464304 26.820507895291286 27.550878279815578 23.320638381428832 88-89 22.125013387934317 26.89820795270954 27.48008654765275 23.496692111703393 90-91 22.14654253186027 26.86920663798884 27.647827452317397 23.336423377833494 92-93 22.271036484568572 26.60003243296245 27.581217543467083 23.547713539001894 94-95 22.032595198389323 26.583694053763494 27.692392400470954 23.691318347376225 96-97 22.04048373718934 26.525393537415564 27.686464364470798 23.7476583609243 98-99 22.072824511772865 26.539779738385278 27.327168247410654 24.060227502431207 100-101 22.19200372913581 26.27836419868711 27.496895529638106 24.032736542538977 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1182.0 1 795.5 2 538.0 3 707.5 4 590.5 5 548.0 6 1097.0 7 2484.0 8 4042.0 9 4526.0 10 3692.0 11 2790.0 12 2626.0 13 2755.0 14 2941.5 15 3037.0 16 3100.5 17 3188.0 18 3281.5 19 3420.5 20 3665.0 21 4166.5 22 4874.0 23 5824.5 24 6931.0 25 8477.5 26 10421.5 27 12747.5 28 15108.5 29 17845.0 30 21141.0 31 24228.0 32 28059.5 33 30979.0 34 33672.0 35 36862.0 36 40636.0 37 44999.5 38 48050.0 39 50090.5 40 52351.5 41 56392.5 42 60657.5 43 64635.0 44 68686.5 45 72080.5 46 72714.5 47 74939.0 48 80297.0 49 80540.0 50 77700.5 51 75050.5 52 72305.0 53 71722.0 54 72178.0 55 68461.5 56 61049.5 57 55154.0 58 50493.5 59 44247.0 60 36448.5 61 29267.0 62 23441.0 63 18150.0 64 14022.5 65 10762.0 66 7712.0 67 6061.5 68 4557.0 69 2978.0 70 2152.0 71 1629.0 72 1281.5 73 999.0 74 777.0 75 575.5 76 347.5 77 173.5 78 96.5 79 59.0 80 29.5 81 13.0 82 5.5 83 1.5 84 1.0 85 1.5 86 1.0 87 1.0 88 1.0 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011313741620288706 3 0.006335695307361675 4 0.0 5 4.5254966481154827E-4 6 1.0056659218034405E-4 7 0.0 8 1.0056659218034405E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.5141648045086013E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016593487709756771 26-27 0.02383428234674154 28-29 3.519830726312042E-4 30-31 2.2627483240577413E-4 32-33 0.003796388854807988 34-35 0.017699720223740553 36-37 0.00882471846382519 38-39 0.02315545784952422 40-41 0.024965656508770414 42-43 0.020289309972384414 44-45 0.01035835899457544 46-47 0.0026901563408242035 48-49 4.022663687213762E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.2627483240577413E-4 68-69 2.2627483240577413E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.2627483240577413E-4 82-83 0.0011313741620288707 84-85 0.004751771480521257 86-87 7.542494413525805E-4 88-89 5.028329609017203E-5 90-91 0.004399788407890052 92-93 7.291077933074945E-4 94-95 1.759915363156021E-4 96-97 3.7712472067629024E-4 98-99 3.0169977654103216E-4 100-101 0.003394122486086612 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1988732.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.73139913334373 #Duplication Level Percentage of deduplicated Percentage of total 1 73.90411399768804 38.97067332808224 2 14.30664859321786 15.08819194458923 3 5.01391318402094 7.931719719796275 4 2.2698177899622864 4.787626713698619 5 1.2312095001422831 3.2461699784383673 6 0.7422388591998254 2.348357612204622 7 0.4864608737436755 1.7956233747293018 8 0.33463150725928176 1.4116470057505275 9 0.25301868846209763 1.2007826504541017 >10 1.3185192954524572 12.521800024053292 >50 0.08817383148651518 3.180498232596917 >100 0.0473344861657015 4.643621618811421 >500 0.0028678486822077435 1.0541941700767619 >1k 7.64759648588731E-4 0.568154855397847 >5k 1.9118991214718274E-4 0.7201870658387934 >10k+ 9.559495607359137E-5 0.5307517054816725 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10529 0.5294328245334213 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8538 0.4293187820178888 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5749 0.289078669222399 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2198 0.11052268480619813 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 5.028329609017203E-5 0.0 18-19 0.0 0.0 0.0 1.2570824022543008E-4 0.0 20-21 0.0 0.0 0.0 2.011331843606881E-4 0.0 22-23 0.0 0.0 0.0 2.5141648045086016E-4 0.0 24-25 5.028329609017203E-5 0.0 0.0 7.039661452624084E-4 0.0 26-27 5.028329609017203E-5 0.0 0.0 0.0015084988827051607 0.0 28-29 1.0056659218034405E-4 0.0 0.0 0.004852338072701601 0.0 30-31 1.0056659218034405E-4 0.0 0.0 0.016316929581260825 0.0 32-33 1.0056659218034405E-4 0.0 0.0 0.03957295402296539 0.0 34-35 1.5084988827051608E-4 0.0 0.0 0.07351417888383151 0.0 36-37 2.5141648045086016E-4 0.0 0.0 0.12885094623106583 0.0 38-39 3.0169977654103216E-4 0.0 0.0 0.21043559413736995 0.0 40-41 3.0169977654103216E-4 0.0 0.0 0.3018003431332125 0.0 42-43 3.268414245861182E-4 0.0 0.0 0.3891927117379315 0.0 44-45 3.519830726312042E-4 0.0 0.0 0.4929522932200015 2.5141648045086013E-5 46-47 3.519830726312042E-4 0.0 0.0 0.6205964403449031 5.028329609017203E-5 48-49 3.519830726312042E-4 0.0 0.0 0.7506541856821332 5.028329609017203E-5 50-51 3.519830726312042E-4 0.0 0.0 0.8900143407960449 5.028329609017203E-5 52-53 3.519830726312042E-4 0.0 0.0 1.0213543101835743 5.028329609017203E-5 54-55 3.519830726312042E-4 0.0 0.0 1.164435429208159 5.028329609017203E-5 56-57 3.519830726312042E-4 0.0 0.0 1.3277807165570827 5.028329609017203E-5 58-59 3.519830726312042E-4 0.0 0.0 1.5000010056659219 5.028329609017203E-5 60-61 3.519830726312042E-4 0.0 0.0 1.6818254043279839 5.028329609017203E-5 62-63 3.519830726312042E-4 0.0 0.0 1.8609093633531315 5.028329609017203E-5 64-65 3.519830726312042E-4 0.0 0.0 2.0450216519872964 5.028329609017203E-5 66-67 4.022663687213762E-4 0.0 0.0 2.2485684345603127 5.028329609017203E-5 68-69 4.022663687213762E-4 0.0 0.0 2.470166920429701 5.028329609017203E-5 70-71 4.022663687213762E-4 0.0 0.0 2.7085600271932067 5.028329609017203E-5 72-73 4.022663687213762E-4 0.0 0.0 2.9383295486772476 5.028329609017203E-5 74-75 4.022663687213762E-4 0.0 0.0 3.1710406429825637 5.028329609017203E-5 76-77 4.022663687213762E-4 0.0 0.0 3.4241164722043997 5.028329609017203E-5 78-79 4.022663687213762E-4 0.0 0.0 3.7041190064825225 5.028329609017203E-5 80-81 4.022663687213762E-4 0.0 0.0 4.00466226721348 5.028329609017203E-5 82-83 4.022663687213762E-4 0.0 0.0 4.305104961352258 5.028329609017203E-5 84-85 4.022663687213762E-4 0.0 0.0 4.6079612537033645 5.028329609017203E-5 86-87 4.022663687213762E-4 0.0 0.0 4.917304091250103 5.028329609017203E-5 88-89 4.5254966481154827E-4 0.0 0.0 5.2537747670374895 5.028329609017203E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6315 0.0 39.79587 1 GTATCAA 10000 0.0 36.342697 1 ACCGTAC 75 3.081212E-4 31.66323 8 TATCAAC 12385 0.0 29.15233 2 ATCAACG 12440 0.0 28.86633 3 TCAACGC 12625 0.0 28.590958 4 CAACGCA 12830 0.0 28.171143 5 AACGCAG 13065 0.0 27.700783 6 ACGCAGA 14990 0.0 24.080109 7 CGCAGAG 15130 0.0 23.700336 8 GTACATG 9095 0.0 23.661987 1 GAGTACT 8110 0.0 22.986101 12-13 TACATGG 9255 0.0 22.944931 2 GCAGAGT 16010 0.0 22.249304 9 GGACCGA 570 0.0 21.664316 6 GTACTTT 8555 0.0 21.651655 14-15 TAAGGTG 750 0.0 21.530996 5 ACATGGG 9430 0.0 21.508312 3 AGTACTT 8400 0.0 21.03343 12-13 AGAGTAC 13495 0.0 20.535934 10-11 >>END_MODULE