FastQCFastQC Report
Thu 26 May 2016
SRR938065_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938065_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031514
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110800.5454060370738277No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89120.43868759949476105No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56710.2791514112135087No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20990.1033219559402495No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA62400.039.925161
GTATCAA101300.037.4086341
TATCAAC126450.029.8729922
TCAACGC127000.029.631384
ATCAACG127350.029.5872553
CAACGCA127600.029.4548135
AACGCAG131000.028.6540666
AATACCG908.933472E-426.3972055
GTACATG89700.025.8053681
ACGCAGA147400.025.3178837
CGCAGAG148200.025.0208248
TACATGG91400.024.79722
GAGTACT78750.023.14815912-13
ACATGGG94150.022.9626263
GCAGAGT158500.022.9144089
GTACTTT83500.021.81871214-15
TACCGTA1100.00286424621.6088927
AGTACTT82400.021.60360712-13
AGAGTAC130400.021.56388510-11
CATGGGG46300.020.7300724
Produced by FastQC (version 0.11.5)