##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938065_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2031514 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78556780804858 33.0 31.0 34.0 30.0 34.0 2 31.977651150816584 34.0 31.0 34.0 30.0 34.0 3 32.0465288449895 34.0 31.0 34.0 30.0 34.0 4 35.57419983322783 37.0 35.0 37.0 33.0 37.0 5 35.589540116386104 37.0 35.0 37.0 33.0 37.0 6 35.61768956551616 37.0 35.0 37.0 33.0 37.0 7 35.55259525654266 37.0 35.0 37.0 33.0 37.0 8 35.537777243966815 37.0 35.0 37.0 33.0 37.0 9 37.17780581379208 39.0 37.0 39.0 34.0 39.0 10-11 37.090346411592535 39.0 37.0 39.0 33.0 39.0 12-13 36.98465479440456 39.0 37.0 39.0 33.0 39.0 14-15 38.206812505353156 40.0 38.0 41.0 33.0 41.0 16-17 38.130914185184054 40.0 38.0 41.0 33.0 41.0 18-19 38.07557097809811 40.0 38.0 41.0 33.0 41.0 20-21 37.97634399762936 40.0 38.0 41.0 33.0 41.0 22-23 37.82137976898018 40.0 38.0 41.0 32.5 41.0 24-25 37.769159848270796 40.0 38.0 41.0 32.5 41.0 26-27 37.77335893328818 40.0 38.0 41.0 32.5 41.0 28-29 37.682154540899056 40.0 38.0 41.0 32.0 41.0 30-31 37.498261641317754 40.0 37.0 41.0 32.0 41.0 32-33 37.27780807811317 40.0 37.0 41.0 31.5 41.0 34-35 37.067692863549055 40.0 36.0 41.0 31.0 41.0 36-37 36.85670317802388 40.0 36.0 41.0 30.5 41.0 38-39 36.62309612436833 39.0 36.0 41.0 30.0 41.0 40-41 36.398133608727285 39.0 35.0 41.0 30.0 41.0 42-43 36.109077761708754 39.0 35.0 41.0 29.5 41.0 44-45 35.86286286976117 39.0 35.0 40.5 28.0 41.0 46-47 35.69030191276063 39.0 35.0 41.0 27.0 41.0 48-49 35.43435314745554 39.0 35.0 41.0 26.0 41.0 50-51 34.79273118472233 38.5 34.5 40.0 25.0 40.5 52-53 34.88247533612862 38.5 34.5 40.0 26.0 41.0 54-55 35.02677978099092 39.0 35.0 40.0 25.5 41.0 56-57 34.82669353989192 38.5 34.5 40.0 25.0 41.0 58-59 34.57110485086493 38.0 34.0 40.0 24.0 41.0 60-61 34.20342168451707 38.0 34.0 40.0 23.0 41.0 62-63 33.781797959551355 37.0 33.5 40.0 22.0 41.0 64-65 33.361153799580016 37.0 33.0 40.0 20.5 41.0 66-67 32.925909198755214 36.0 32.5 39.0 19.5 41.0 68-69 32.440367873418545 35.5 32.0 39.0 17.5 40.5 70-71 31.968039353900586 35.0 31.5 38.5 13.5 40.0 72-73 31.47564230421252 35.0 31.0 37.5 9.5 40.0 74-75 30.94663388979845 35.0 31.0 37.0 7.0 39.0 76-77 30.41127676206022 35.0 30.0 36.5 3.5 39.0 78-79 29.846179991868134 34.0 29.5 36.0 2.0 38.5 80-81 29.308446311470163 34.0 29.0 35.0 2.0 37.0 82-83 28.773279928171796 34.0 29.0 35.0 2.0 37.0 84-85 28.27978468275385 34.0 27.5 35.0 2.0 36.0 86-87 27.778799949200447 33.5 26.5 35.0 2.0 36.0 88-89 27.2335243567113 33.0 26.0 35.0 2.0 35.5 90-91 26.62720439042015 33.0 25.0 35.0 2.0 35.0 92-93 26.016600673192507 32.0 23.5 35.0 2.0 35.0 94-95 25.41656493629874 32.0 21.0 34.5 2.0 35.0 96-97 24.572673385465226 32.0 12.5 34.0 2.0 35.0 98-99 23.509907881511033 31.0 2.0 34.0 2.0 35.0 100-101 20.704494529695587 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.44481816041178845 1101 2 0.42169740496630226 1101 3 0.375672719305868 1101 4 0.19579871706125118 1101 5 0.24724051942982328 1101 6 0.29869917193006046 1101 7 0.2556941234908905 1101 8 0.21477455158897385 1101 9 0.2760934760614049 1101 10-11 0.3365334014514687 1101 12-13 0.22634837122703289 1101 14-15 0.36426555173942177 1101 16-17 0.516133286586502 1101 18-19 0.5579517595245562 1101 20-21 0.564633228478101 1101 22-23 0.6253111871507713 1101 24-25 0.5604856574234134 1101 26-27 0.47827196312427844 1101 28-29 0.54860821327906 1101 30-31 0.6539666200808867 1101 32-33 0.7632884348885298 1101 34-35 0.750514903759786 1101 36-37 0.7337388195034578 1101 38-39 0.693237451348736 1101 40-41 0.7815995595559713 1101 42-43 0.8463841289966254 1101 44-45 0.7897374451857218 1101 46-47 0.890149337089504 1101 48-49 1.0734848613707655 1101 50-51 0.9951510949038891 1101 52-53 1.0175735930393586 1101 54-55 1.1398260826158548 1101 56-57 0.9904263080064837 1101 58-59 0.8738605816916305 1101 60-61 0.8470813086988542 1101 62-63 1.0557498899525086 1101 64-65 1.132992380518992 1101 66-67 1.0514263769053187 1101 68-69 1.0463342765005166 1101 70-71 1.1587483710758875 1101 72-73 1.148562505682687 1101 74-75 1.147559974697895 1101 76-77 1.0955612114224849 1101 78-79 1.2312207321191657 1101 80-81 1.337438778449446 1101 82-83 1.1813713977466769 1101 84-85 1.1904576547899985 1101 86-87 1.2113697466656106 1101 88-89 1.3138916918706407 1101 90-91 1.4232031212056135 1101 92-93 1.4726571356484897 1101 94-95 1.4518657475766226 1101 96-97 1.350765488122736 1101 98-99 1.4335665384875647 1101 100-101 1.1344147444630828 1102 1 0.4781389168667367 1102 2 0.37560845986526203 1102 3 0.38567842318592227 1102 4 0.38602754539217443 1102 5 0.36040053983624887 1102 6 0.36137453967089783 1102 7 0.2992384649584565 1102 8 0.3599737268291747 1102 9 0.4896462139238267 1102 10-11 0.5755746890403941 1102 12-13 0.42503692166208396 1102 14-15 0.533368324321124 1102 16-17 0.5161430292387692 1102 18-19 0.45416195636155976 1102 20-21 0.5594253379926073 1102 22-23 0.6196799341394126 1102 24-25 0.606014842866827 1102 26-27 0.6259006015620585 1102 28-29 0.44700120707211255 1102 30-31 0.4883003466630882 1102 32-33 0.5867878893000054 1102 34-35 0.5929784308124724 1102 36-37 0.5771960970875654 1102 38-39 0.49792295853350055 1102 40-41 0.6356935991784596 1102 42-43 0.7615428988007835 1102 44-45 0.8040662295861409 1102 46-47 0.7056080034444037 1102 48-49 0.6543068046579066 1102 50-51 0.6058205391121447 1102 52-53 0.5281456992944911 1102 54-55 0.4636993005961827 1102 56-57 0.38859699218866695 1102 58-59 0.3836594756348859 1102 60-61 0.45186087676280096 1102 62-63 0.44254381337171367 1102 64-65 0.3804309486679571 1102 66-67 0.40984526843949 1102 68-69 0.4613349496292187 1102 70-71 0.3688181107522226 1102 72-73 0.4786271791798349 1102 74-75 0.33084583474380835 1102 76-77 0.3912861356069399 1102 78-79 0.5097396895490895 1102 80-81 0.8218784342326835 1102 82-83 0.8500849070854564 1102 84-85 0.7901502298261356 1102 86-87 0.6618447452484979 1102 88-89 0.659323627930501 1102 90-91 0.8777549769151598 1102 92-93 1.0788812733351953 1102 94-95 1.1062801433196157 1102 96-97 1.1520942087530663 1102 98-99 1.315399237662806 1102 100-101 1.408876330070882 1103 1 0.3167661159305091 1103 2 0.35589670735613765 1103 3 0.37651624051045474 1103 4 0.2550152870294795 1103 5 0.18509450478691747 1103 6 0.15321982395381895 1103 7 0.16613410138839413 1103 8 0.16529430569865866 1103 9 0.31552658146269863 1103 10-11 0.2841254376443132 1103 12-13 0.14236102836198938 1103 14-15 0.26121825584220204 1103 16-17 0.39332195214804955 1103 18-19 0.31472021856585997 1103 20-21 0.388082599938258 1103 22-23 0.42312338496815016 1103 24-25 0.4323438215344808 1103 26-27 0.22422530612745817 1103 28-29 0.4295448067312009 1103 30-31 0.3568136120687271 1103 32-33 0.40840602709739926 1103 34-35 0.5449448146590825 1103 36-37 0.45285618723380594 1103 38-39 0.49271856061797337 1103 40-41 0.5589997529528503 1103 42-43 0.613749700898822 1103 44-45 0.636334005484116 1103 46-47 0.6734364796506327 1103 48-49 0.6835937334525468 1103 50-51 0.5476376261927385 1103 52-53 0.5981307314562656 1103 54-55 0.8460622685531334 1103 56-57 0.7954313156042119 1103 58-59 0.8070415286456267 1103 60-61 0.7702043229273414 1103 62-63 0.8007491992493669 1103 64-65 0.6407386161869013 1103 66-67 0.7886854941867085 1103 68-69 0.7897233841120652 1103 70-71 0.9137322258900937 1103 72-73 0.8633137662159065 1103 74-75 0.6971075641439519 1103 76-77 0.8255947105245696 1103 78-79 0.9772556644302597 1103 80-81 1.0362848725232112 1103 82-83 1.0433196986169655 1103 84-85 1.010714682694406 1103 86-87 1.1124848005404573 1103 88-89 1.1982921615487747 1103 90-91 1.273132755823653 1103 92-93 1.4651396953943099 1103 94-95 1.3906957828024815 1103 96-97 1.381274709009599 1103 98-99 1.3703935346196268 1103 100-101 1.6736764691488126 1104 1 0.38910788580450273 1104 2 0.37156982439731223 1104 3 0.21398759772843334 1104 4 0.23077979437362472 1104 5 0.2571830051514894 1104 6 0.3004733827236734 1104 7 0.2540468807013383 1104 8 0.35268574972143085 1104 9 0.27923732635498766 1104 10-11 0.3308691249017883 1104 12-13 0.34843576419523004 1104 14-15 0.3992696002367495 1104 16-17 0.46479394120679984 1104 18-19 0.5467714749825774 1104 20-21 0.47192653793254635 1104 22-23 0.4441963412498353 1104 24-25 0.6550409018702297 1104 26-27 0.7395217180983096 1104 28-29 0.7580252296646179 1104 30-31 0.6605746642835655 1104 32-33 0.7254839901173895 1104 34-35 0.6039775384126926 1104 36-37 0.6267013115957951 1104 38-39 0.493284284722165 1104 40-41 0.4920991452393295 1104 42-43 0.5986307108843079 1104 44-45 0.6296614203089064 1104 46-47 0.6438156774261259 1104 48-49 0.5808416269833785 1104 50-51 0.591199048714266 1104 52-53 0.567886752938854 1104 54-55 0.6327885468643331 1104 56-57 0.5953238760123227 1104 58-59 0.547583578715674 1104 60-61 0.5034284837721472 1104 62-63 0.557266823474734 1104 64-65 0.6118009751391114 1104 66-67 0.6807669563249235 1104 68-69 0.7004681430994566 1104 70-71 0.6621869486332912 1104 72-73 0.7566525115187552 1104 74-75 0.6467591852687598 1104 76-77 0.5628630522357909 1104 78-79 0.47669847150954325 1104 80-81 0.6908660513994676 1104 82-83 0.6644214476428765 1104 84-85 0.5613923119318365 1104 86-87 0.5245982812766421 1104 88-89 0.5319570113399621 1104 90-91 0.7906316794282375 1104 92-93 0.7886757643049478 1104 94-95 0.7978634432187235 1104 96-97 0.8003288407017415 1104 98-99 1.0213157688832588 1104 100-101 0.7346948258562556 1105 1 0.4345281155822711 1105 2 0.32740966627066825 1105 3 0.2645194907184276 1105 4 0.2628196554637654 1105 5 0.2417629126364247 1105 6 0.21922484113994045 1105 7 0.1689522380647972 1105 8 0.23335778793536832 1105 9 0.4020517609012657 1105 10-11 0.40662990345586536 1105 12-13 0.25107532605267124 1105 14-15 0.3755533497048589 1105 16-17 0.4352728507918542 1105 18-19 0.43436978939653414 1105 20-21 0.4354530914032324 1105 22-23 0.39433840815168253 1105 24-25 0.43408500437801933 1105 26-27 0.31030809614725996 1105 28-29 0.404614937706846 1105 30-31 0.4432672095459438 1105 32-33 0.3249788633552768 1105 34-35 0.3821500227304284 1105 36-37 0.3543007241114111 1105 38-39 0.38458594697355153 1105 40-41 0.3730388457321041 1105 42-43 0.40956505813150557 1105 44-45 0.4688663947094156 1105 46-47 0.41942821225654114 1105 48-49 0.4789679910315314 1105 50-51 0.20529592873293012 1105 52-53 0.36859640113453196 1105 54-55 0.5373731212813055 1105 56-57 0.4898608202699464 1105 58-59 0.4541817970457416 1105 60-61 0.5655082883100491 1105 62-63 0.565351596922639 1105 64-65 0.48422626991077067 1105 66-67 0.6319371209029043 1105 68-69 0.45925913317536526 1105 70-71 0.5987825589719122 1105 72-73 0.623839861387669 1105 74-75 0.7766248050785158 1105 76-77 0.7525340335268851 1105 78-79 0.7736114332269715 1105 80-81 0.8075160177701051 1105 82-83 0.8633013839529831 1105 84-85 0.7723390128226733 1105 86-87 0.8798273041034328 1105 88-89 0.7978252665936871 1105 90-91 0.9409782677636507 1105 92-93 0.9408424561364406 1105 94-95 1.170914655934741 1105 96-97 1.2135788098176334 1105 98-99 1.1015787642300516 1105 100-101 1.1438996629940554 1106 1 0.13102998042946012 1106 2 0.04737502076839917 1106 3 -0.01859318376115482 1106 4 0.1815924504955575 1106 5 0.1357959908421691 1106 6 0.2036815018555913 1106 7 0.25298997102338916 1106 8 0.15500710239044224 1106 9 0.1408140680089005 1106 10-11 -0.4747257491228041 1106 12-13 -0.24947794140707202 1106 14-15 0.07216068300283496 1106 16-17 0.16776066889118368 1106 18-19 0.1431195463477266 1106 20-21 0.06311233992579446 1106 22-23 -0.013637127881409583 1106 24-25 0.03622795831953596 1106 26-27 0.08715511975687207 1106 28-29 0.017584611108169668 1106 30-31 0.21627848111836556 1106 32-33 0.1081869897288712 1106 34-35 -0.1458197390522855 1106 36-37 -0.1138690152813524 1106 38-39 0.04164320043367553 1106 40-41 0.30680465863460427 1106 42-43 0.056461987762247645 1106 44-45 0.2520197246253346 1106 46-47 0.4046549694257422 1106 48-49 0.4681006161353807 1106 50-51 0.5723756310644319 1106 52-53 0.4709644329989686 1106 54-55 0.022827313210321165 1106 56-57 0.1852838430310939 1106 58-59 0.43093926085355605 1106 60-61 0.37001927411599667 1106 62-63 0.37866419009134233 1106 64-65 0.3217182419351232 1106 66-67 0.03785749516860193 1106 68-69 -0.2844660832117185 1106 70-71 -0.048548028646440144 1106 72-73 0.3123479247158798 1106 74-75 0.4371819613191228 1106 76-77 0.5542716115234576 1106 78-79 0.6492169930051936 1106 80-81 0.5733701649192184 1106 82-83 0.6635900964719106 1106 84-85 0.7885711376878923 1106 86-87 0.5465469996001033 1106 88-89 0.3544034929560915 1106 90-91 0.4107524238687468 1106 92-93 0.339147890413102 1106 94-95 0.5291727177899261 1106 96-97 0.5898266076481669 1106 98-99 0.6302096187601904 1106 100-101 0.3143248952897899 1107 1 0.3031822446190411 1107 2 0.331943483590301 1107 3 0.19630379273138487 1107 4 0.16832836274569019 1107 5 0.21162169518769502 1107 6 0.2842733792636025 1107 7 0.07821604308221453 1107 8 0.158686697212012 1107 9 0.3133396406445854 1107 10-11 0.2114113701132041 1107 12-13 0.17695880933232644 1107 14-15 0.21434979685407995 1107 16-17 0.18342597146014583 1107 18-19 0.2681100625675086 1107 20-21 0.26039298484522533 1107 22-23 0.292715158464965 1107 24-25 0.2892744618539922 1107 26-27 0.29865539224070403 1107 28-29 0.42404107915817946 1107 30-31 0.43820587620649576 1107 32-33 0.4364749519862201 1107 34-35 0.43354310758127923 1107 36-37 0.30944340337914866 1107 38-39 0.4497670233538784 1107 40-41 0.49197851674829707 1107 42-43 0.49975871762054425 1107 44-45 0.4754036493101239 1107 46-47 0.6816247701600489 1107 48-49 0.6171936981793138 1107 50-51 0.5016655728860542 1107 52-53 0.4200218179822528 1107 54-55 0.5609715808107083 1107 56-57 0.4427806174267843 1107 58-59 0.4808289804358097 1107 60-61 0.4180401015044808 1107 62-63 0.537630922445075 1107 64-65 0.5331804673950629 1107 66-67 0.6414765504610003 1107 68-69 0.6112792523910073 1107 70-71 0.5755916889775037 1107 72-73 0.7576732323652315 1107 74-75 0.5753859851276069 1107 76-77 0.6788827765345182 1107 78-79 0.6758593095765768 1107 80-81 0.6473670383490777 1107 82-83 0.5955460156376482 1107 84-85 0.4431010275417542 1107 86-87 0.6514739772900029 1107 88-89 0.7436702037125436 1107 90-91 0.8685260463146989 1107 92-93 1.0183078568869561 1107 94-95 1.1321703756598964 1107 96-97 0.9902048398267738 1107 98-99 0.9511176893172753 1107 100-101 0.8729556948032346 1108 1 0.20195465445084793 1108 2 0.15605354449041897 1108 3 0.059833123261093135 1108 4 0.1540994966964817 1108 5 0.027353206897785753 1108 6 0.1241307409563035 1108 7 0.08931062232515785 1108 8 0.11437171249311717 1108 9 0.16659265343461982 1108 10-11 0.1624780787668172 1108 12-13 0.019446108796685735 1108 14-15 0.04269366283951115 1108 16-17 0.16928913509089938 1108 18-19 0.18669931024631836 1108 20-21 0.17438569635845624 1108 22-23 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66-67 -1.0920515730068523 2316 68-69 -1.1878048662774958 2316 70-71 -1.3808896501560426 2316 72-73 -1.193454496081177 2316 74-75 -1.3276266155833767 2316 76-77 -1.2293878589060157 2316 78-79 -1.1484892375234672 2316 80-81 -1.1903918043171942 2316 82-83 -1.1630473389059226 2316 84-85 -1.2493231335174961 2316 86-87 -1.3119372972997212 2316 88-89 -1.142380766383429 2316 90-91 -1.1450771459610571 2316 92-93 -1.275412957792426 2316 94-95 -1.5151583150987626 2316 96-97 -1.6522016891161648 2316 98-99 -1.7448220385064381 2316 100-101 -1.718267194727435 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6519.0 3 1808.0 4 1886.0 5 2088.0 6 2335.0 7 2886.0 8 3375.0 9 4190.0 10 5176.0 11 6221.0 12 7606.0 13 9468.0 14 10676.0 15 12965.0 16 16793.0 17 22473.0 18 19828.0 19 15099.0 20 15080.0 21 16034.0 22 16803.0 23 18415.0 24 20604.0 25 22880.0 26 26279.0 27 30481.0 28 35658.0 29 42109.0 30 50240.0 31 61233.0 32 76089.0 33 99068.0 34 135223.0 35 196990.0 36 289509.0 37 394716.0 38 298845.0 39 33865.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.93780332751208 18.566537873578287 14.567932994612459 22.927725804297168 2 15.457338364690667 19.802547789522208 40.406093970546664 24.33401987524046 3 19.323066890475516 24.181157107507055 29.473270997840718 27.02250500417672 4 12.755204577427326 16.485841342648968 36.69069461356283 34.06825946636087 5 13.874655201548519 37.27892624379929 33.69824451463167 15.148174040020516 6 32.3163676431745 35.64835073859 17.271612676861455 14.76366894137405 7 29.00931069525539 32.83900590680635 20.067673843404364 18.084009554533896 8 26.78355251687587 34.6890831130991 19.23794851690345 19.28941585312158 9 26.839452241000174 16.669662711067225 20.00758450692086 36.483300541011744 10-11 25.451484463229868 26.497819519518412 26.49220359689991 21.558492420351808 12-13 26.242338944746773 23.862403825636676 27.407593025427534 22.48766420418902 14-15 23.02303056631123 25.240780594816542 25.977190334446703 25.758998504425527 16-17 22.25745922671946 28.576871329986076 26.59772670012937 22.56794274316509 18-19 22.178256786765612 26.827373782573776 29.161783252510215 21.832586178150397 20-21 23.01195919395826 25.84932740771977 29.343166771308283 21.795546627013696 22-23 22.65662250611772 25.763557498689764 29.358407777090367 22.221412218102145 24-25 22.406813898776896 26.008671339007478 29.40978235431781 22.174732407897814 26-27 22.122137506104956 26.350669556160284 29.33964036986109 22.187552567873666 28-29 21.947949241286636 26.581407107825694 29.467525107573984 22.00311854331369 30-31 22.203596348135484 26.156515305304374 29.330776394356445 22.309111952203697 32-33 21.98585647812907 26.303000030541178 29.26086403953408 22.450279451795673 34-35 22.599927782931058 26.348786768020155 29.01020250828698 22.041082940761804 36-37 22.54798492973589 26.12839181307434 29.037670980677955 22.28595227651181 38-39 22.17337648523433 26.386940968829776 29.0514001386744 22.388282407261496 40-41 22.39223061717159 26.184830388863116 29.113221503546267 22.309717490419022 42-43 22.324787848464315 26.19790763824322 28.83827828293175 22.639026230360717 44-45 22.23930964093116 26.517127272029022 28.74106562143197 22.502497465607842 46-47 22.40630240111753 26.739166240133414 28.369052569330428 22.48547878941863 48-49 22.522454874215036 26.559026720334 28.326636405959604 22.59188199949136 50-51 22.45889802061051 26.856485460407903 28.14984284482986 22.534773674151733 52-53 22.613985249945163 26.9927185236848 27.885762285167893 22.507533941202144 54-55 22.39715979268121 26.82120957952041 28.016003123617505 22.765627504180866 56-57 22.438388505998972 26.972349453279154 28.036642544396834 22.552619496325036 58-59 22.584335467461138 26.63976912326465 28.060469498269487 22.71542591100473 60-61 22.497713449349362 26.75848978099584 28.232739466171974 22.511057303482822 62-63 22.32427804348233 26.845276252315276 28.2320275242999 22.598418179902495 64-65 22.160909976034475 26.67068759023999 28.256523435624807 22.91187899810073 66-67 22.215428106812357 26.83742051069593 28.041160523530902 22.905990858960813 68-69 22.187717398849994 26.68004846594263 28.219915445540554 22.91231868966682 70-71 22.261142513269178 26.712533843909767 28.079044796581975 22.947278846239076 72-73 22.33718122206661 26.722833826753412 27.939025687390313 23.000959263789664 74-75 22.162342490508806 27.00583012856812 27.82468251897008 23.007144861952995 76-77 22.272287057866613 26.744838710153896 27.835786026125618 23.14708820585387 78-79 22.32805824391906 26.83796922186195 27.825793060395377 23.008179473823617 80-81 22.3068490898085 27.17251925459539 27.654293392597307 22.866338262998806 82-83 22.379548933484255 27.010053405794928 27.658583995592434 22.951813665128384 84-85 22.455737671019417 26.741482200293532 27.663407887928336 23.13937224075871 86-87 22.430481420974992 26.751708688410115 27.67018024579711 23.147629644817787 88-89 22.33405009872452 26.813132048080156 27.582023592932416 23.270794260262907 90-91 22.428003705642734 26.78373457275033 27.67554645178798 23.11271526981896 92-93 22.583293878564994 26.515204673611198 27.659749956482806 23.241751491341 94-95 22.378606025846587 26.48884815259627 27.722223686080135 23.410322135477013 96-97 22.455680630670116 26.333422305306502 27.78720300679306 23.423694057230318 98-99 22.496322275744994 26.292718536838176 27.40276958795947 23.808189599457357 100-101 22.73640591123872 25.949067807850717 27.57970759377516 23.734818687135398 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1294.0 1 858.0 2 573.5 3 761.5 4 628.5 5 601.5 6 1159.5 7 2475.5 8 4077.5 9 4737.0 10 3849.5 11 2856.5 12 2780.0 13 2913.0 14 3078.5 15 3108.0 16 3141.5 17 3282.5 18 3428.5 19 3654.0 20 3919.5 21 4471.0 22 5275.0 23 6180.5 24 7289.0 25 8811.0 26 10880.5 27 13392.0 28 15682.0 29 18207.0 30 21352.5 31 24648.0 32 28537.5 33 31517.0 34 34407.5 35 37900.5 36 41588.0 37 45842.5 38 49245.0 39 51084.0 40 53373.5 41 57424.5 42 61813.5 43 66162.0 44 69932.0 45 73406.5 46 74018.0 47 75760.5 48 80978.5 49 81371.5 50 78306.5 51 75425.0 52 73327.0 53 72592.5 54 72199.0 55 68645.5 56 61762.0 57 56079.0 58 51168.0 59 44643.5 60 37029.0 61 30418.0 62 24800.5 63 19360.5 64 15174.0 65 11972.0 66 8972.5 67 7010.0 68 5312.5 69 3633.5 70 2704.0 71 2088.5 72 1619.0 73 1260.0 74 977.0 75 784.0 76 499.5 77 267.5 78 146.5 79 83.5 80 54.0 81 29.5 82 16.5 83 13.0 84 11.0 85 8.0 86 6.0 87 4.5 88 4.5 89 4.0 90 5.0 91 4.0 92 4.0 93 3.5 94 2.0 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5013010001407816 2 0.051587141412759156 3 0.05956148960824291 4 0.06783118403318904 5 0.08392755353888774 6 0.11036104107576909 7 0.07299974304878036 8 0.05414680873476629 9 0.052128609500106816 10-11 0.0773560999333502 12-13 0.11109940664942501 14-15 0.05860161436249024 16-17 0.06367172463492746 18-19 0.06189964725815327 20-21 0.07725765119019608 22-23 0.0662560041427231 24-25 0.09052361933021381 26-27 0.0689387323936729 28-29 0.06888950802209584 30-31 0.07297513086299183 32-33 0.07226137747512446 34-35 0.07496871791186278 36-37 0.07007089293994528 38-39 0.04223451081311771 40-41 0.04568021682351192 42-43 0.043809690703583634 44-45 0.02239708906756242 46-47 0.029805356989909985 48-49 0.02990380573306411 50-51 0.027541035897365215 52-53 0.02650732409424695 54-55 0.02635965097951577 56-57 0.026654997208978133 58-59 0.023307739941738036 60-61 0.030346825077257653 62-63 0.021461826007598276 64-65 0.023209291198583913 66-67 0.01695779600829726 68-69 0.019542075516092924 70-71 0.04272675452888831 72-73 0.03888725354587761 74-75 0.03366947015870922 76-77 0.02367692272856599 78-79 0.016342491363584007 80-81 0.018483751527186128 82-83 0.02269243529702478 84-85 0.0189513830571682 86-87 0.010066383987508825 88-89 0.04250524485679154 90-91 0.05399913562003511 92-93 0.03482624289077014 94-95 0.054663664636325424 96-97 0.04489262687827896 98-99 0.03477701851919308 100-101 0.1695779600829726 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2031514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.39294915270849 #Duplication Level Percentage of deduplicated Percentage of total 1 75.4099101452387 41.01767408140285 2 13.619371609679595 14.815955749142878 3 4.6761289985296415 7.630453405455856 4 2.0491342511486494 4.458338205192076 5 1.143401555085965 3.1096491323459365 6 0.6943365404846907 2.2660207284870784 7 0.46796769488235584 1.7817900122992332 8 0.3381429507124938 1.4714073859561192 9 0.24174120723273618 1.183411547381213 >10 1.2282365027668467 11.97577716911432 >50 0.0832786156405432 3.091533319865088 >100 0.044352662170847164 4.2928706199337725 >500 0.002997949820167211 1.0896850933778122 >1k 7.267757139799258E-4 0.5473132638423996 >5k 1.8169392849498144E-4 0.7206093649885982 >10k+ 9.084696424749072E-5 0.5475109212146793 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11080 0.5454060370738277 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8912 0.43868759949476105 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5671 0.2791514112135087 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2099 0.1033219559402495 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.461218578853013E-5 0.0 12-13 0.0 0.0 0.0 4.922437157706026E-5 0.0 14-15 0.0 0.0 0.0 4.922437157706026E-5 0.0 16-17 0.0 0.0 0.0 1.2306092894265064E-4 0.0 18-19 0.0 0.0 0.0 1.476731147311808E-4 0.0 20-21 0.0 0.0 0.0 1.7228530051971092E-4 0.0 22-23 0.0 0.0 0.0 3.199584152508917E-4 0.0 24-25 0.0 0.0 0.0 6.399168305017834E-4 0.0 26-27 0.0 0.0 0.0 0.0014521189615232777 0.0 28-29 0.0 0.0 0.0 0.005094722458225737 0.0 30-31 4.922437157706026E-5 0.0 0.0 0.015456452675196922 0.0 32-33 4.922437157706026E-5 0.0 0.0 0.03832117327274141 0.0 34-35 4.922437157706026E-5 0.0 0.0 0.07482104479713159 0.0 36-37 4.922437157706026E-5 0.0 0.0 0.1332257616733136 0.0 38-39 4.922437157706026E-5 0.0 0.0 0.21503666723438775 0.0 40-41 4.922437157706026E-5 0.0 0.0 0.30440351383254066 0.0 42-43 7.38365573655904E-5 0.0 0.0 0.39089073469343555 0.0 44-45 1.2306092894265064E-4 0.0 0.0 0.49704309199936597 0.0 46-47 1.476731147311808E-4 0.0 0.0 0.6170028855326619 0.0 48-49 1.476731147311808E-4 0.0 0.0 0.7427465427262623 0.0 50-51 1.476731147311808E-4 0.0 0.0 0.8801071516120489 0.0 52-53 1.476731147311808E-4 0.0 0.0 1.0106747972202013 0.0 54-55 1.476731147311808E-4 0.0 0.0 1.1538684941378694 0.0 56-57 1.476731147311808E-4 0.0 0.0 1.3159889619269176 0.0 58-59 1.476731147311808E-4 0.0 0.0 1.4864775728840658 2.461218578853013E-5 60-61 1.476731147311808E-4 0.0 0.0 1.6668110581566262 4.922437157706026E-5 62-63 1.476731147311808E-4 0.0 0.0 1.8406715385668029 4.922437157706026E-5 64-65 1.476731147311808E-4 0.0 0.0 2.019232946462589 4.922437157706026E-5 66-67 1.9689748630824104E-4 0.0 0.0 2.2234894763216007 4.922437157706026E-5 68-69 1.9689748630824104E-4 0.0 0.0 2.444949924046795 4.922437157706026E-5 70-71 2.2150967209677116E-4 0.0 0.0 2.6802916445567195 4.922437157706026E-5 72-73 2.461218578853013E-4 0.0 0.0 2.9158056503671643 4.922437157706026E-5 74-75 2.461218578853013E-4 0.0 0.0 3.1580633950836665 4.922437157706026E-5 76-77 2.461218578853013E-4 0.0 0.0 3.4121596011644515 4.922437157706026E-5 78-79 2.461218578853013E-4 0.0 0.0 3.6912864001921717 4.922437157706026E-5 80-81 2.953462294623616E-4 4.922437157706026E-5 0.0 3.9900044991075623 4.922437157706026E-5 82-83 3.4457060103942183E-4 4.922437157706026E-5 0.0 4.288944107695049 4.922437157706026E-5 84-85 3.69182786827952E-4 4.922437157706026E-5 0.0 4.584314949343199 4.922437157706026E-5 86-87 3.937949726164821E-4 4.922437157706026E-5 0.0 4.885863449624271 4.922437157706026E-5 88-89 3.937949726164821E-4 4.922437157706026E-5 0.0 5.215715963562151 4.922437157706026E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6240 0.0 39.92516 1 GTATCAA 10130 0.0 37.408634 1 TATCAAC 12645 0.0 29.872992 2 TCAACGC 12700 0.0 29.63138 4 ATCAACG 12735 0.0 29.587255 3 CAACGCA 12760 0.0 29.454813 5 AACGCAG 13100 0.0 28.654066 6 AATACCG 90 8.933472E-4 26.397205 5 GTACATG 8970 0.0 25.805368 1 ACGCAGA 14740 0.0 25.317883 7 CGCAGAG 14820 0.0 25.020824 8 TACATGG 9140 0.0 24.7972 2 GAGTACT 7875 0.0 23.148159 12-13 ACATGGG 9415 0.0 22.962626 3 GCAGAGT 15850 0.0 22.914408 9 GTACTTT 8350 0.0 21.818712 14-15 TACCGTA 110 0.002864246 21.608892 7 AGTACTT 8240 0.0 21.603607 12-13 AGAGTAC 13040 0.0 21.563885 10-11 CATGGGG 4630 0.0 20.730072 4 >>END_MODULE