FastQCFastQC Report
Thu 26 May 2016
SRR938065_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938065_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031514
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105770.5206461781705664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88180.43406050856651734No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59830.2945094151455515No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22990.11316683025566153No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64800.041.787571
GTATCAA104600.035.970031
ATCAACG128700.028.9362353
TATCAAC130200.028.7151452
TCAACGC130300.028.5788084
CAACGCA132100.028.2253465
AACGCAG135000.027.6190266
ACGCAGA153750.024.127287
CGCAGAG155100.023.8254018
GTACATG96950.023.4221841
GAGTACT82000.023.19856612-13
TACATGG97650.022.767792
GCAGAGT167200.021.7318979
GTACTTT87850.021.51858714-15
ACATGGG101250.021.2054733
AGTACTT86550.020.74422312-13
AGAGTAC138750.020.69358610-11
GTGTAGG9700.019.590051
CAGAGTA161600.019.16367110-11
ACTTTTT100850.019.02733416-17
Produced by FastQC (version 0.11.5)