##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938065_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2031514 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.493795760206424 34.0 31.0 34.0 31.0 34.0 2 32.70632149224667 34.0 31.0 34.0 31.0 34.0 3 32.76509539190968 34.0 31.0 34.0 31.0 34.0 4 36.232406471232785 37.0 35.0 37.0 35.0 37.0 5 36.19571364017181 37.0 35.0 37.0 35.0 37.0 6 36.234213990157095 37.0 37.0 37.0 35.0 37.0 7 36.19571413241553 37.0 37.0 37.0 35.0 37.0 8 36.18900140486357 37.0 37.0 37.0 35.0 37.0 9 37.88988065058867 39.0 38.0 39.0 35.0 39.0 10-11 37.8726334152755 39.0 38.0 39.0 35.0 39.0 12-13 37.82504255446923 39.0 38.0 39.0 35.0 39.0 14-15 39.139360595102964 40.5 38.5 41.0 36.0 41.0 16-17 39.10187451329403 40.0 38.0 41.0 36.0 41.0 18-19 39.12704022714094 40.0 38.5 41.0 36.0 41.0 20-21 39.05906949201433 40.0 39.0 41.0 35.5 41.0 22-23 38.91727967417404 40.0 38.0 41.0 35.0 41.0 24-25 38.88404953153166 40.0 38.0 41.0 35.0 41.0 26-27 38.85294169766982 40.0 38.0 41.0 35.0 41.0 28-29 38.79501273434492 40.0 38.0 41.0 35.0 41.0 30-31 38.607302484747834 40.0 38.0 41.0 34.5 41.0 32-33 38.44362185050164 40.0 38.0 41.0 34.0 41.0 34-35 38.259661513531285 40.0 38.0 41.0 34.0 41.0 36-37 38.14508957358896 40.0 38.0 41.0 34.0 41.0 38-39 38.140272230464575 40.0 38.0 41.0 34.0 41.0 40-41 38.07897533563637 40.0 38.0 41.0 33.5 41.0 42-43 37.92920403206672 40.0 38.0 41.0 33.0 41.0 44-45 37.72079296524661 40.0 37.0 41.0 33.0 41.0 46-47 37.38693063400006 40.0 37.0 41.0 32.5 41.0 48-49 37.04950938068849 40.0 37.0 41.0 31.5 41.0 50-51 36.76464498890975 40.0 36.0 41.0 31.0 41.0 52-53 36.361542672115476 39.0 35.5 41.0 30.0 41.0 54-55 36.152718120574114 39.0 35.0 41.0 30.0 41.0 56-57 35.92518880007718 39.0 35.0 40.5 29.0 41.0 58-59 35.61241886592955 38.5 35.0 40.0 28.5 41.0 60-61 35.26337918419465 38.0 35.0 40.0 28.0 41.0 62-63 34.913038502318955 37.5 34.0 40.0 27.5 41.0 64-65 34.537075550549986 37.0 34.0 40.0 26.5 41.0 66-67 34.11784979084564 36.5 34.0 39.0 26.0 41.0 68-69 33.669589527810295 36.0 33.0 39.0 26.0 40.5 70-71 33.16735449521884 35.5 33.0 38.5 25.0 40.0 72-73 32.76481998155071 35.0 32.5 37.5 24.0 40.0 74-75 32.323494940226844 35.0 32.0 37.0 23.0 39.0 76-77 31.016901926346556 34.0 30.5 35.5 21.5 38.5 78-79 31.727186472748897 35.0 32.0 36.0 23.0 39.0 80-81 31.569385689687593 35.0 32.0 36.0 22.5 37.5 82-83 31.209040646532586 35.0 31.5 35.0 20.5 37.0 84-85 30.834293536741562 34.0 31.0 35.0 19.5 36.5 86-87 30.409491640224974 34.0 31.0 35.0 17.5 36.0 88-89 29.973726245548885 34.0 30.5 35.0 13.0 36.0 90-91 29.649443961498665 34.0 30.0 35.0 7.0 35.0 92-93 29.261343756429934 34.0 30.0 35.0 2.0 35.0 94-95 28.863917994165927 34.0 29.0 35.0 2.0 35.0 96-97 28.298326765161352 34.0 29.0 35.0 2.0 35.0 98-99 27.63911890343852 34.0 28.5 35.0 2.0 35.0 100-101 25.238541796906148 31.0 22.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13276667933983788 1101 2 0.12419672274163673 1101 3 0.15765991448967753 1101 4 0.043218669664291554 1101 5 0.03511851536008237 1101 6 0.01370171152142774 1101 7 0.07312526132973574 1101 8 0.12309192332965324 1101 9 0.10031897000943957 1101 10-11 0.10854813171684441 1101 12-13 0.16929907855079307 1101 14-15 0.19137559824400796 1101 16-17 0.29049593485302694 1101 18-19 0.18880215067651562 1101 20-21 0.17964352671324235 1101 22-23 0.23690081282526876 1101 24-25 0.1445130722119501 1101 26-27 0.1468200634905159 1101 28-29 0.17960697666450898 1101 30-31 0.05897908238974736 1101 32-33 0.047444228691205126 1101 34-35 0.02035863311504471 1101 36-37 0.018049578823813306 1101 38-39 -0.06066103746233864 1101 40-41 0.13789000861591916 1101 42-43 0.06907966067063143 1101 44-45 0.08277356414455284 1101 46-47 0.40193195513791125 1101 48-49 0.34246452489790613 1101 50-51 0.3446822978860382 1101 52-53 0.2920448528183357 1101 54-55 0.3294734795674188 1101 56-57 0.35944155650204834 1101 58-59 0.19730130915119304 1101 60-61 0.2672532236587273 1101 62-63 0.26726758981299525 1101 64-65 0.17567760710461044 1101 66-67 0.16913849472700093 1101 68-69 0.18818143709633262 1101 70-71 0.10168187477337653 1101 72-73 0.14742512354408888 1101 74-75 0.16914472181227325 1101 76-77 0.21987757835658428 1101 78-79 0.3585829344574023 1101 80-81 0.502969379638234 1101 82-83 0.3931294926842064 1101 84-85 0.32666485449524885 1101 86-87 0.3907337647796716 1101 88-89 0.3248011257316321 1101 90-91 0.3969577785501208 1101 92-93 0.5292107638253398 1101 94-95 0.30521513343377293 1101 96-97 0.4061983832160223 1101 98-99 0.2739016095605855 1101 100-101 0.4736389279299189 1102 1 0.22542550228573077 1102 2 0.23526118721461842 1102 3 0.1620503078802642 1102 4 0.12494889385157393 1102 5 0.12246493573250206 1102 6 0.07486873955192408 1102 7 0.1296140448798866 1102 8 0.18903285097185574 1102 9 0.21666483770776068 1102 10-11 0.2228861273521403 1102 12-13 0.19988170687037865 1102 14-15 0.2424992803288717 1102 16-17 0.27901731908906413 1102 18-19 0.2888211045636595 1102 20-21 0.2779867444101498 1102 22-23 0.32796759782088003 1102 24-25 0.23392316347403153 1102 26-27 0.048735911774393514 1102 28-29 0.18533299911158707 1102 30-31 0.09969096913407327 1102 32-33 0.16825045973732244 1102 34-35 0.2550786115749233 1102 36-37 0.3456958603191538 1102 38-39 0.23325263909578098 1102 40-41 0.35072710438441845 1102 42-43 0.40047110626765914 1102 44-45 0.3715803195225007 1102 46-47 0.25622527628407 1102 48-49 0.2371730247420203 1102 50-51 0.33124496616820664 1102 52-53 0.30472890037664513 1102 54-55 0.3957385718214823 1102 56-57 0.45349535011370534 1102 58-59 0.4551454727710649 1102 60-61 0.36446186521415314 1102 62-63 0.3809235997894689 1102 64-65 0.48655146987137954 1102 66-67 0.3912390814118041 1102 68-69 0.5726814087540717 1102 70-71 0.6334252072564439 1102 72-73 0.4630263869003741 1102 74-75 0.5275295140635023 1102 76-77 0.5517070004578812 1102 78-79 0.4334003040121388 1102 80-81 0.6112797734539548 1102 82-83 0.6016576790629173 1102 84-85 0.5800513118543158 1102 86-87 0.6088553637083365 1102 88-89 0.29190727458518495 1102 90-91 0.4138382521493007 1102 92-93 0.5163571055479821 1102 94-95 0.4520656882335352 1102 96-97 0.6355851452543995 1102 98-99 0.5194766740392289 1102 100-101 0.65461312230115 1103 1 0.06852524405871918 1103 2 0.10995960435511876 1103 3 0.10006649689056246 1103 4 0.06020133301557706 1103 5 0.09383777907054025 1103 6 0.08160214666440169 1103 7 0.1464390822994801 1103 8 0.08220488782750834 1103 9 0.07361725015862675 1103 10-11 0.05673676274322759 1103 12-13 0.13044832223085479 1103 14-15 0.15664833964476088 1103 16-17 0.24394254350063704 1103 18-19 0.153934593002802 1103 20-21 0.1546771984911075 1103 22-23 0.19650460823900318 1103 24-25 0.13317604361684232 1103 26-27 0.07932584812927956 1103 28-29 0.0379031847042981 1103 30-31 -0.10295108752322335 1103 32-33 0.13306119926725302 1103 34-35 0.02081795070034076 1103 36-37 -0.023355484030169293 1103 38-39 -0.04011520930927759 1103 40-41 -0.008068514660784842 1103 42-43 0.048088760684443344 1103 44-45 0.10744461677613515 1103 46-47 -0.007902794136853686 1103 48-49 0.2682001237375289 1103 50-51 0.20258091993328264 1103 52-53 0.004975678899285185 1103 54-55 0.20119695656700998 1103 56-57 0.2265502731756186 1103 58-59 0.3399664735385386 1103 60-61 0.36796637464643567 1103 62-63 0.45319451342122363 1103 64-65 0.2736210385177955 1103 66-67 0.31234542938648957 1103 68-69 0.30857962745411527 1103 70-71 0.34580399108778437 1103 72-73 0.2290968841463794 1103 74-75 0.24437999256790022 1103 76-77 0.3513304724027222 1103 78-79 0.3122583385187312 1103 80-81 0.3767023710735167 1103 82-83 0.29641723961057664 1103 84-85 0.5357817041982464 1103 86-87 0.49118229012848147 1103 88-89 0.3707441081877718 1103 90-91 0.46452494943007494 1103 92-93 0.46216377874452874 1103 94-95 0.4767465086237159 1103 96-97 0.3152522062578811 1103 98-99 0.41758512938376313 1103 100-101 0.41369452953235 1104 1 0.10941653945228325 1104 2 0.09273594471734725 1104 3 0.10202760269190492 1104 4 -0.029682682879084155 1104 5 0.028382807056821946 1104 6 0.006609044926044305 1104 7 0.006093116149614275 1104 8 0.03810160185557265 1104 9 0.04101906490131313 1104 10-11 0.060265206908631797 1104 12-13 0.09773269990102307 1104 14-15 0.1209786617369275 1104 16-17 0.16098411569110738 1104 18-19 0.07310896106205433 1104 20-21 0.1281928058913806 1104 22-23 0.1513874444309522 1104 24-25 0.13210111449897255 1104 26-27 0.1857626260612406 1104 28-29 0.1805121805706733 1104 30-31 0.14383658864904447 1104 32-33 0.12554709282207455 1104 34-35 0.27693980799230644 1104 36-37 0.27674382427746735 1104 38-39 0.14829237864523037 1104 40-41 0.1535814162233038 1104 42-43 0.06087087292548432 1104 44-45 0.12987563082725728 1104 46-47 -0.0639284090152401 1104 48-49 -0.20361817805041937 1104 50-51 -0.08516523470241566 1104 52-53 -0.10177342032770298 1104 54-55 -0.09574821325019656 1104 56-57 -0.04520324493781658 1104 58-59 0.008910166394606733 1104 60-61 0.024734868985859748 1104 62-63 0.12643445695545807 1104 64-65 0.00840227364653856 1104 66-67 0.026325106507847806 1104 68-69 0.09582390856194678 1104 70-71 0.08823663139575899 1104 72-73 -0.05795717822990554 1104 74-75 -0.1061760673050145 1104 76-77 0.05957546133218017 1104 78-79 0.1799843382334707 1104 80-81 0.09839850364810587 1104 82-83 0.08686484681356177 1104 84-85 0.24784626392919407 1104 86-87 0.21988119133870754 1104 88-89 0.19376763959119003 1104 90-91 0.04426098928136568 1104 92-93 0.38732917039916614 1104 94-95 0.2541849941253709 1104 96-97 0.3148403100557218 1104 98-99 0.47508463950721236 1104 100-101 0.6478161975386811 1105 1 0.08861849407323064 1105 2 0.04535011297397773 1105 3 0.049436769095478894 1105 4 0.05599669888968606 1105 5 0.016515073401379254 1105 6 0.001519343746956281 1105 7 0.02880734891971315 1105 8 0.05653598341844912 1105 9 -0.012532607644772042 1105 10-11 0.03659837095129603 1105 12-13 0.032281723276859964 1105 14-15 0.1148457639839151 1105 16-17 0.006888882824924281 1105 18-19 -0.01037696264733512 1105 20-21 0.0963217779309602 1105 22-23 0.03363114064112693 1105 24-25 0.060056037425972875 1105 26-27 -0.03259351575859171 1105 28-29 -0.10341572905556262 1105 30-31 0.014403346366641756 1105 32-33 -0.03734256168769434 1105 34-35 0.1144413521977583 1105 36-37 0.11866248239724797 1105 38-39 0.07792602447999286 1105 40-41 0.0587122962145159 1105 42-43 0.06938665127367472 1105 44-45 0.19401300334451577 1105 46-47 0.19017094173215554 1105 48-49 0.24723628230124461 1105 50-51 0.2325073388360721 1105 52-53 0.1675634635343144 1105 54-55 0.18959487488618976 1105 56-57 0.24547112674213878 1105 58-59 0.29051559101679203 1105 60-61 0.1726656336764023 1105 62-63 0.37872605785040747 1105 64-65 0.26792350132419074 1105 66-67 0.4598913289008877 1105 68-69 0.45387230739246576 1105 70-71 0.3959919743466358 1105 72-73 0.3539338900857487 1105 74-75 0.33225749068162 1105 76-77 0.21682087332619915 1105 78-79 0.2653205632344964 1105 80-81 0.38879972254094497 1105 82-83 0.4471533730238768 1105 84-85 0.4885240671768294 1105 86-87 0.51245116100365 1105 88-89 0.4559201933638555 1105 90-91 0.5454757642391534 1105 92-93 0.45692410815052753 1105 94-95 0.35603804067115163 1105 96-97 0.5020501710086016 1105 98-99 0.4629410078105103 1105 100-101 0.29383214746524544 1106 1 -0.18692519873664537 1106 2 -0.10530125782128152 1106 3 0.005634955509492556 1106 4 -0.07523551435691189 1106 5 -0.07455663925306766 1106 6 -0.044589813153571356 1106 7 -0.03813388924454131 1106 8 -0.1385016965113195 1106 9 -0.19060224039066753 1106 10-11 -0.14033644483244245 1106 12-13 -0.25115442706576374 1106 14-15 -0.4645000959374812 1106 16-17 -0.27656199774826007 1106 18-19 -0.15371094411081287 1106 20-21 -0.06699994798069042 1106 22-23 -0.14251236715706028 1106 24-25 -0.12891160909563837 1106 26-27 -0.15633570137128316 1106 28-29 -0.15180394722795398 1106 30-31 -0.1228664937808972 1106 32-33 -0.018161444664848148 1106 34-35 -0.07346630693292155 1106 36-37 -0.16417385295707732 1106 38-39 -0.20853983968370926 1106 40-41 -0.21337450608810826 1106 42-43 -0.3890947208767628 1106 44-45 -0.21197984365552003 1106 46-47 -0.1818581180666854 1106 48-49 -0.21438262895794935 1106 50-51 -0.12307099947607725 1106 52-53 -0.21926005062732656 1106 54-55 -0.45353406176793243 1106 56-57 -0.35055465705342925 1106 58-59 -0.25785259404204197 1106 60-61 -0.2868156265242874 1106 62-63 -0.3120805106540061 1106 64-65 -0.4130308997740926 1106 66-67 -0.34024242159257057 1106 68-69 -0.6023382524152154 1106 70-71 -0.5669285000846358 1106 72-73 -0.29987269609494405 1106 74-75 -0.29814158072464636 1106 76-77 -0.17606619294216586 1106 78-79 -0.2690857200926473 1106 80-81 -0.4184599242870455 1106 82-83 -0.41299192597034207 1106 84-85 -0.17820269883051054 1106 86-87 -0.06976296941192217 1106 88-89 -0.11726548413836824 1106 90-91 -0.23351126726586102 1106 92-93 -0.2382025423718055 1106 94-95 -0.32925058346639346 1106 96-97 -0.28209479802845294 1106 98-99 -0.21481163037378437 1106 100-101 -0.23675959535671254 1107 1 0.049076461904043356 1107 2 0.10633307447023554 1107 3 0.04231318175724397 1107 4 0.05339408779430954 1107 5 0.07787271917339211 1107 6 0.08774556912390352 1107 7 0.04607446968660156 1107 8 0.030249611355124273 1107 9 0.042234355474967344 1107 10-11 0.013890187860468473 1107 12-13 0.04213564456335206 1107 14-15 0.13283829093584387 1107 16-17 0.13127087734224574 1107 18-19 0.12484519371341918 1107 20-21 0.07582913919287648 1107 22-23 0.12517575019786875 1107 24-25 0.12437022647183937 1107 26-27 0.07999383509287128 1107 28-29 0.1099014994273233 1107 30-31 0.12780117553675296 1107 32-33 0.11927156739493938 1107 34-35 0.1705958395235001 1107 36-37 0.07937096587754411 1107 38-39 0.12484731899811408 1107 40-41 0.07814760986355651 1107 42-43 0.20120339993597014 1107 44-45 0.1950165564245978 1107 46-47 0.24914897546520365 1107 48-49 0.2118434084109353 1107 50-51 0.2237240423252942 1107 52-53 0.2803127036750652 1107 54-55 0.3217855052569334 1107 56-57 0.32391176141236144 1107 58-59 0.39409423859917325 1107 60-61 0.25146180565969445 1107 62-63 0.3826381148189313 1107 64-65 0.3100671127165171 1107 66-67 0.21149078819983202 1107 68-69 0.3164248612189269 1107 70-71 0.2611115970024187 1107 72-73 0.27039523328530635 1107 74-75 0.2899405247528932 1107 76-77 0.15245027526451338 1107 78-79 0.22071387280419685 1107 80-81 0.3981069808449327 1107 82-83 0.41978502559020114 1107 84-85 0.5361252508489436 1107 86-87 0.5631559870701217 1107 88-89 0.5701063729256788 1107 90-91 0.4199106708719178 1107 92-93 0.6424152965348533 1107 94-95 0.505377355806079 1107 96-97 0.6810724376650263 1107 98-99 0.6952663548348745 1107 100-101 0.6560939426248495 1108 1 -0.0020247496331577963 1108 2 -0.03867821318441855 1108 3 0.01979086923280704 1108 4 0.025159118099232103 1108 5 0.0061762108476202116 1108 6 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2316 96-97 -0.2748050087547611 2316 98-99 -0.10800623962198941 2316 100-101 0.0769017979216855 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 20.0 5 36.0 6 54.0 7 169.0 8 406.0 9 760.0 10 1253.0 11 2038.0 12 3135.0 13 4743.0 14 6467.0 15 8767.0 16 11572.0 17 17367.0 18 16919.0 19 11474.0 20 9491.0 21 9682.0 22 9963.0 23 10618.0 24 12222.0 25 14479.0 26 17482.0 27 21300.0 28 25806.0 29 32240.0 30 39475.0 31 49122.0 32 62037.0 33 81126.0 34 114155.0 35 172413.0 36 284554.0 37 479760.0 38 447821.0 39 52580.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.31149083885221 19.055935622397875 14.880970547089511 22.75160299166041 2 15.748330768314286 20.08990859473388 39.950984012201815 24.21077662475002 3 19.34862807144327 24.109346678651985 29.77752988863695 26.76449536126779 4 12.998581353611149 16.865746433448155 36.48047712198883 33.65519509095187 5 13.814819883101961 37.39993915867673 33.79927482655793 14.98596613166338 6 32.38953804896249 35.86847051017123 17.02646400664726 14.71552743421901 7 29.033371170466953 32.87306905096396 19.94133439395446 18.152225384614628 8 26.77077641754163 34.95409818312486 19.16790958449626 19.10721581483724 9 26.592314674616098 16.725014127436367 20.025911785727647 36.656759412219884 10-11 25.47683648746698 26.59824150854978 26.43095248174514 21.493969522238093 12-13 26.17887447489902 23.859471310559513 27.451890560439164 22.50976365410231 14-15 22.9005313278668 25.252890208977146 25.998344092140147 25.848234371015906 16-17 22.240336853380274 28.66569005176945 26.561759324481777 22.5322137703685 18-19 22.137627404979735 26.907690520468968 29.182693301645962 21.77198877290533 20-21 22.87591914207827 25.91126617882033 29.417518166254332 21.795296512847067 22-23 22.646459733971806 25.799822201569867 29.39096161778851 22.162756446669825 24-25 22.451454518424292 26.03222220800101 29.443278851862313 22.073044421712382 26-27 22.09903513293817 26.393301615858146 29.44011418684951 22.067549064354175 28-29 21.937757243552653 26.583997595881097 29.53467943973791 21.943565720828335 30-31 22.136298346947154 26.18852245172812 29.39120773964639 22.283971461678334 32-33 21.935718561223606 26.291112397514354 29.334276476935315 22.438892564326725 34-35 22.55909869262575 26.35510714915213 29.1112819002961 21.974512257926023 36-37 22.534545322976403 26.111196300331958 29.15898220239925 22.195276174292385 38-39 22.19871854204864 26.373919071410707 29.154940811097696 22.27242157544296 40-41 22.392444729994857 26.16680574940982 29.174463916028486 22.26628560456684 42-43 22.316933348364586 26.244742958160767 28.906284229539075 22.532039463935575 44-45 22.258460625133374 26.468621374488084 28.826857800654654 22.44606019972389 46-47 22.383341873766007 26.697072575956938 28.460438106967565 22.459147443309494 48-49 22.455493784438602 26.55374267664412 28.466281797713428 22.52448174120385 50-51 22.432210656682653 26.824575169061106 28.260917719493932 22.48229645476231 52-53 22.64116811402727 26.914680381232913 27.95855701708184 22.485594487657973 54-55 22.34045642806301 26.811850669008436 28.088533970231065 22.759158932697485 56-57 22.384216894395017 26.9642739355968 28.106673151157214 22.544836018850965 58-59 22.541070354425322 26.642986462313328 28.101947611485816 22.713995571775534 60-61 22.39539082674301 26.868286411021536 28.239825076273164 22.49649768596229 62-63 22.274914177308155 26.875325496157053 28.24024348343157 22.609516843103222 64-65 22.048186721824216 26.775178512183523 28.19328343294705 22.983351333045206 66-67 22.11372897257907 26.95730868701865 27.99321097467209 22.93575136573019 68-69 22.160579529369894 26.770671379744282 28.160745415787908 22.908003675097913 70-71 22.181633993169626 26.84074537512417 28.002612829643308 22.975007802062894 72-73 22.246240980864517 26.834124697147054 27.838104979832774 23.081529342155655 74-75 22.153699162299645 27.073970447656283 27.611500585277778 23.160829804766298 76-77 22.18089562759597 26.834444655562308 27.74755674831677 23.237102968524955 78-79 22.239891036931077 26.91002867811888 27.803967878144082 23.046112406805957 80-81 22.20819054163545 27.183150103814203 27.627473893854532 22.981185460695816 82-83 22.234993211959157 27.058120200008467 27.61583232997656 23.091054258055816 84-85 22.335310240536838 26.842397767906366 27.588586708252606 23.233705283304193 86-87 22.25716346817668 26.873838146143342 27.564139009222473 23.30485937645751 88-89 22.114606727745954 26.9507325207204 27.46480762486079 23.46985312667286 90-91 22.1264177566397 26.976651117481303 27.609480728748387 23.28745039713061 92-93 22.239297664419155 26.655648576242026 27.616535996052193 23.488517763286623 94-95 22.012179094015597 26.604960635270047 27.731977234712634 23.650883036001723 96-97 22.054561277943446 26.549632441617433 27.75174081990082 23.6440654605383 98-99 22.01360660079133 26.610892172045087 27.392181397716186 23.983319829447396 100-101 21.967163406208375 26.523937319654205 27.602172566863924 23.906726707273492 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1215.0 1 808.5 2 545.0 3 725.5 4 614.5 5 560.0 6 1101.0 7 2546.0 8 4105.5 9 4544.5 10 3728.0 11 2850.5 12 2679.5 13 2810.0 14 3003.5 15 3120.0 16 3222.5 17 3236.5 18 3280.0 19 3451.0 20 3798.0 21 4337.5 22 4969.5 23 5891.5 24 7084.0 25 8737.5 26 10780.0 27 13079.5 28 15441.5 29 18116.5 30 21294.0 31 24736.0 32 28761.5 33 31756.5 34 34407.5 35 37567.5 36 41573.5 37 46127.0 38 49347.5 39 51544.5 40 53786.5 41 57727.5 42 62102.0 43 66349.5 44 70596.0 45 73976.0 46 74373.5 47 76538.0 48 82244.5 49 82587.0 50 79307.0 51 76240.0 52 73761.5 53 73196.0 54 73650.0 55 69907.0 56 62206.0 57 56336.0 58 51595.5 59 44709.0 60 36604.5 61 29840.5 62 24067.0 63 18595.5 64 14296.5 65 10906.0 66 7750.0 67 6063.0 68 4492.5 69 2862.0 70 2118.5 71 1599.5 72 1245.0 73 966.0 74 755.0 75 583.0 76 350.0 77 173.0 78 87.0 79 45.5 80 19.5 81 7.0 82 4.0 83 3.0 84 0.5 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016736286336200487 3 0.0068914120207884365 4 0.0 5 0.0 6 0.0 7 0.0 8 1.9689748630824104E-4 9 2.953462294623616E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.4457060103942183E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005906924589247231 26-27 0.02249553781071654 28-29 2.461218578853013E-5 30-31 0.0 32-33 4.922437157706026E-4 34-35 0.02067423606236531 36-37 0.004356356884569833 38-39 0.019197504915053502 40-41 0.01717930568039403 42-43 0.00940185497121851 44-45 0.0029288501088350852 46-47 4.922437157706026E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.7228530051971092E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.002855013551469495 86-87 4.184071584050122E-4 88-89 7.38365573655904E-5 90-91 1.7228530051971092E-4 92-93 3.199584152508917E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2031514.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.07166311297765 #Duplication Level Percentage of deduplicated Percentage of total 1 73.4068919353537 38.22418947028494 2 14.521505082825845 15.123178411325998 3 5.052962768258272 7.893485249734912 4 2.3412706103918164 4.876554179225529 5 1.2458043778405903 3.2435552933793965 6 0.8057010766135538 2.5172517018710625 7 0.5195661466391593 1.8938271346891578 8 0.35355597749032797 1.4728198201164695 9 0.2582983161640942 1.2105020611751494 >10 1.3496438830444832 12.602477750320798 >50 0.09087582812905971 3.2265048533127025 >100 0.049762882011304396 4.773831582613831 >500 0.00302626562753029 1.0652097009218466 >1k 8.511372077428926E-4 0.6268836157706574 >5k 1.891416017206428E-4 0.7288691592318539 >10k+ 9.45708008603214E-5 0.5208600160256279 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10577 0.5206461781705664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8818 0.43406050856651734 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5983 0.2945094151455515 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2299 0.11316683025566153 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.461218578853013E-5 0.0 12-13 4.922437157706026E-5 0.0 0.0 4.922437157706026E-5 0.0 14-15 9.844874315412052E-5 0.0 0.0 4.922437157706026E-5 0.0 16-17 1.2306092894265064E-4 0.0 0.0 7.38365573655904E-5 0.0 18-19 1.476731147311808E-4 0.0 0.0 9.844874315412052E-5 0.0 20-21 1.476731147311808E-4 0.0 0.0 1.2306092894265064E-4 0.0 22-23 1.476731147311808E-4 0.0 0.0 2.7073404367383144E-4 0.0 24-25 1.9689748630824104E-4 0.0 0.0 5.906924589247232E-4 0.0 26-27 1.9689748630824104E-4 0.0 0.0 0.0013782824041576873 0.0 28-29 1.9689748630824104E-4 0.0 0.0 0.004996273715071616 0.0 30-31 1.9689748630824104E-4 0.0 0.0 0.015308779560465741 0.0 32-33 1.9689748630824104E-4 0.0 0.0 0.03824733671537582 0.0 34-35 1.9689748630824104E-4 0.0 0.0 0.0749933300976513 0.0 36-37 2.461218578853013E-4 0.0 0.0 0.13376722976066124 0.0 38-39 2.461218578853013E-4 0.0 0.0 0.21626727652381428 0.0 40-41 2.461218578853013E-4 0.0 0.0 0.3063971008814116 0.0 42-43 2.461218578853013E-4 0.0 0.0 0.3933027289007115 0.0 44-45 2.953462294623616E-4 0.0 0.0 0.4998242689934699 0.0 46-47 2.953462294623616E-4 0.0 0.0 0.6205716524719986 0.0 48-49 2.953462294623616E-4 0.0 0.0 0.7475213067692371 0.0 50-51 2.953462294623616E-4 0.0 0.0 0.8857187299718339 0.0 52-53 2.953462294623616E-4 0.0 0.0 1.0169755167820649 0.0 54-55 2.953462294623616E-4 0.0 0.0 1.1606860696012924 0.0 56-57 2.953462294623616E-4 0.0 0.0 1.323298781106111 0.0 58-59 2.953462294623616E-4 0.0 0.0 1.494894940423743 4.922437157706026E-5 60-61 2.953462294623616E-4 0.0 0.0 1.6762621374994215 4.922437157706026E-5 62-63 2.953462294623616E-4 0.0 0.0 1.8508855956690429 4.922437157706026E-5 64-65 2.953462294623616E-4 0.0 0.0 2.0302099813242735 4.922437157706026E-5 66-67 3.4457060103942183E-4 0.0 0.0 2.234934142713267 4.922437157706026E-5 68-69 3.4457060103942183E-4 0.0 0.0 2.456763773225289 4.922437157706026E-5 70-71 3.4457060103942183E-4 0.0 0.0 2.693483776139372 4.922437157706026E-5 72-73 3.4457060103942183E-4 0.0 0.0 2.930769859326591 4.922437157706026E-5 74-75 3.4457060103942183E-4 0.0 0.0 3.1758580054087737 4.922437157706026E-5 76-77 3.4457060103942183E-4 0.0 0.0 3.4321939203963154 4.922437157706026E-5 78-79 3.4457060103942183E-4 0.0 0.0 3.713880386746043 4.922437157706026E-5 80-81 3.937949726164821E-4 0.0 0.0 4.015773457628153 4.922437157706026E-5 82-83 4.4301934419354233E-4 0.0 0.0 4.318872525613902 4.922437157706026E-5 84-85 4.4301934419354233E-4 0.0 0.0 4.6177875220156 4.922437157706026E-5 86-87 4.4301934419354233E-4 0.0 0.0 4.9241107863396465 4.922437157706026E-5 88-89 4.4301934419354233E-4 0.0 0.0 5.259845612680985 4.922437157706026E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6480 0.0 41.78757 1 GTATCAA 10460 0.0 35.97003 1 ATCAACG 12870 0.0 28.936235 3 TATCAAC 13020 0.0 28.715145 2 TCAACGC 13030 0.0 28.578808 4 CAACGCA 13210 0.0 28.225346 5 AACGCAG 13500 0.0 27.619026 6 ACGCAGA 15375 0.0 24.12728 7 CGCAGAG 15510 0.0 23.825401 8 GTACATG 9695 0.0 23.422184 1 GAGTACT 8200 0.0 23.198566 12-13 TACATGG 9765 0.0 22.76779 2 GCAGAGT 16720 0.0 21.731897 9 GTACTTT 8785 0.0 21.518587 14-15 ACATGGG 10125 0.0 21.205473 3 AGTACTT 8655 0.0 20.744223 12-13 AGAGTAC 13875 0.0 20.693586 10-11 GTGTAGG 970 0.0 19.59005 1 CAGAGTA 16160 0.0 19.163671 10-11 ACTTTTT 10085 0.0 19.027334 16-17 >>END_MODULE