FastQCFastQC Report
Thu 26 May 2016
SRR938064_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938064_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951771
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96430.4940641089554051No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80970.4148539967035067No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51240.26253079895131143No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19650.10067779467980617No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57750.041.973371
GTATCAA96800.039.8490261
TATCAAC119850.031.9040342
ATCAACG119950.031.8774363
GACCGTA600.003935817631.6925987
TCAACGC121850.031.380374
CAACGCA123200.031.0751155
AACGCAG126050.030.4039846
ACGCAGA142400.026.6404787
CGCAGAG144400.026.0712878
GTACATG90200.024.7613531
GCAGAGT153600.023.9824039
TACATGG91300.023.9615352
GAGTACT82350.022.82785412-13
ACATGGG94750.022.2357653
AGTACTT84650.021.646112-13
GTACTTT86850.021.53533214-15
AGAGTAC130450.021.42391210-11
TCGCGTA1353.780251E-421.1251439
CATGGGG49750.020.9352724
Produced by FastQC (version 0.11.5)