##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938064_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1951771 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.883887505245237 33.0 31.0 34.0 30.0 34.0 2 32.00306183461072 33.0 31.0 34.0 30.0 34.0 3 32.02274908275612 34.0 31.0 34.0 30.0 34.0 4 35.54295867701693 37.0 35.0 37.0 33.0 37.0 5 35.54779940884458 37.0 35.0 37.0 33.0 37.0 6 35.57424667135643 37.0 35.0 37.0 33.0 37.0 7 35.50302161472837 37.0 35.0 37.0 33.0 37.0 8 35.498039472868484 37.0 35.0 37.0 33.0 37.0 9 37.115659572767505 39.0 37.0 39.0 34.0 39.0 10-11 37.015348368225574 39.0 37.0 39.0 33.0 39.0 12-13 36.860007654586525 39.0 37.0 39.0 33.0 39.0 14-15 38.01793729899666 40.0 38.0 41.0 33.0 41.0 16-17 37.84779208216538 40.0 38.0 41.0 33.0 41.0 18-19 38.022547214811574 40.0 38.0 41.0 33.0 41.0 20-21 38.024048671693556 40.0 38.0 41.0 33.0 41.0 22-23 37.90627793936891 40.0 38.0 41.0 33.0 41.0 24-25 37.78510773036386 40.0 38.0 41.0 32.5 41.0 26-27 37.595181504387554 40.0 37.5 41.0 32.0 41.0 28-29 37.41634828061284 40.0 37.0 41.0 32.0 41.0 30-31 37.196606056755634 40.0 37.0 41.0 31.0 41.0 32-33 36.91123471964693 39.5 36.0 41.0 30.5 41.0 34-35 36.67268214355065 39.0 36.0 41.0 30.0 41.0 36-37 36.41477073898526 39.0 35.0 41.0 30.0 41.0 38-39 36.12349630156407 39.0 35.0 40.0 29.5 41.0 40-41 36.01082837074637 39.0 35.0 40.0 29.0 41.0 42-43 35.79087710597196 39.0 35.0 40.5 28.0 41.0 44-45 35.449072662725285 39.0 35.0 40.0 26.5 41.0 46-47 35.04349716232079 38.0 34.5 40.0 25.0 41.0 48-49 34.63427676710024 38.0 34.0 40.0 24.0 41.0 50-51 33.90626846079791 37.5 33.5 39.5 23.0 40.5 52-53 33.994165042927676 38.0 33.5 40.0 23.0 40.5 54-55 34.08586560615974 38.0 34.0 40.0 22.5 41.0 56-57 33.7754757089843 38.0 33.0 40.0 20.5 41.0 58-59 33.36954489025608 37.5 33.0 40.0 18.5 41.0 60-61 32.871131654277065 37.0 32.0 40.0 14.5 41.0 62-63 32.32921177740626 36.0 31.5 39.0 10.0 41.0 64-65 31.791076412140562 35.5 31.0 39.0 7.5 40.5 66-67 31.222014519121352 35.0 30.5 39.0 2.0 40.0 68-69 30.54395802581348 35.0 29.5 38.0 2.0 40.0 70-71 29.96547597028545 34.5 29.0 37.5 2.0 40.0 72-73 29.26299755452868 34.0 28.0 37.0 2.0 39.0 74-75 28.586634907476338 34.0 26.5 36.0 2.0 39.0 76-77 27.842898321575635 33.5 26.0 35.5 2.0 38.5 78-79 27.0832651473969 33.0 25.0 35.0 2.0 37.0 80-81 26.35185813294695 32.0 23.0 35.0 2.0 37.0 82-83 25.61356685799717 32.0 20.0 35.0 2.0 36.0 84-85 24.873337855721804 31.0 17.5 34.5 2.0 36.0 86-87 24.12274954387579 31.0 9.5 34.0 2.0 35.0 88-89 23.251307402354065 30.5 4.0 34.0 2.0 35.0 90-91 22.35661509470117 29.5 2.0 34.0 2.0 35.0 92-93 21.407086948212672 29.0 2.0 34.0 2.0 35.0 94-95 20.32323489794653 28.0 2.0 34.0 2.0 35.0 96-97 19.05127215231705 25.5 2.0 33.5 2.0 35.0 98-99 17.50314714174972 22.0 2.0 33.0 2.0 34.5 100-101 13.665379545038839 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.31142304408547616 1101 2 0.3676622955612885 1101 3 0.26245961506109694 1101 4 0.11936462554505312 1101 5 0.1736998493586981 1101 6 0.12807989386142538 1101 7 0.2096922627245803 1101 8 0.29043035056670874 1101 9 0.2618220931698758 1101 10-11 0.34878116487613653 1101 12-13 0.36563287263850697 1101 14-15 0.4902093096781144 1101 16-17 0.5085697849764941 1101 18-19 0.4547704634778711 1101 20-21 0.5880364905213966 1101 22-23 0.5533386234484468 1101 24-25 0.6405124870806134 1101 26-27 0.5810260685413269 1101 28-29 0.5871874458254567 1101 30-31 0.43324487626924935 1101 32-33 0.5492662114730962 1101 34-35 0.4837665188172551 1101 36-37 0.46574124186413 1101 38-39 0.5418656428212998 1101 40-41 0.7113519016913656 1101 42-43 0.7579466000035993 1101 44-45 0.9228027086547357 1101 46-47 0.9008463838678509 1101 48-49 1.0176702023355375 1101 50-51 1.01751821028936 1101 52-53 0.9977147170865948 1101 54-55 0.897174522972982 1101 56-57 0.8787240268504561 1101 58-59 0.9615853239804011 1101 60-61 0.788110551729936 1101 62-63 0.7053641034172955 1101 64-65 0.5535094168977857 1101 66-67 0.587164181315714 1101 68-69 0.7570054003547604 1101 70-71 0.5171497651780683 1101 72-73 0.5604950768225336 1101 74-75 0.5207873745485649 1101 76-77 0.40323010971572515 1101 78-79 0.1819465488516805 1101 80-81 0.25581385837646664 1101 82-83 0.10430951075480621 1101 84-85 0.18627173016357546 1101 86-87 0.4033516387940459 1101 88-89 0.22132581528097717 1101 90-91 0.08732515009911523 1101 92-93 -0.11479022973255226 1101 94-95 -0.5352126491560867 1101 96-97 -0.8456831153839843 1101 98-99 -0.847343031948828 1101 100-101 -0.7305158561039118 1102 1 0.27985161551405113 1102 2 0.24588451778350873 1102 3 0.2624278690293522 1102 4 0.18109478427520997 1102 5 0.11973159539044786 1102 6 0.21246084624237938 1102 7 0.17191448494680373 1102 8 0.23108114421750514 1102 9 0.34996495031273156 1102 10-11 0.34743195852692565 1102 12-13 0.3260931900988311 1102 14-15 0.3637172461860487 1102 16-17 0.42114121354791934 1102 18-19 0.4012545904620026 1102 20-21 0.4222190302039408 1102 22-23 0.47176719487701746 1102 24-25 0.2863537569218906 1102 26-27 0.3888594018746616 1102 28-29 0.2360287156667269 1102 30-31 0.27447503499940495 1102 32-33 0.17888525909214792 1102 34-35 0.22389350294423593 1102 36-37 0.31134441646730693 1102 38-39 0.21650849996415644 1102 40-41 0.3445185683580334 1102 42-43 0.4656291396861363 1102 44-45 0.35204080389283376 1102 46-47 0.2391162251376855 1102 48-49 0.35313845630379603 1102 50-51 0.21028011505126898 1102 52-53 0.18750043137231387 1102 54-55 0.06758722138567208 1102 56-57 0.17317640780283483 1102 58-59 0.1804583398534092 1102 60-61 0.34744388506326374 1102 62-63 0.26428473833792765 1102 64-65 0.009985607373973693 1102 66-67 -0.13751835836682602 1102 68-69 -0.2190580917087317 1102 70-71 -0.5019057903774851 1102 72-73 -0.5944414311139745 1102 74-75 -0.8091570698958783 1102 76-77 -0.6508889379033214 1102 78-79 -0.6839820225768953 1102 80-81 -0.5483686813060764 1102 82-83 -0.748301600356303 1102 84-85 -0.7009822380903934 1102 86-87 -0.6983070913646863 1102 88-89 -0.7824281529729902 1102 90-91 -0.9269367546627869 1102 92-93 -0.9569013408436611 1102 94-95 -1.183799950743392 1102 96-97 -1.3614053376062074 1102 98-99 -1.5579382700440654 1102 100-101 -1.3117301418181952 1103 1 0.28447884403916746 1103 2 0.27107450517771525 1103 3 0.19686511016027453 1103 4 0.19907875690261534 1103 5 0.1861101295161447 1103 6 0.13458942538878915 1103 7 0.18769334322161058 1103 8 0.14161689066087035 1103 9 0.19695306484541675 1103 10-11 0.22277591674770747 1103 12-13 0.21053493330069983 1103 14-15 0.3144042622134222 1103 16-17 0.24566849609789898 1103 18-19 0.3417700775860766 1103 20-21 0.399445304290019 1103 22-23 0.4092847529537309 1103 24-25 0.328291448259904 1103 26-27 0.33250742996758476 1103 28-29 0.35017872467086875 1103 30-31 0.23997791632481125 1103 32-33 0.24085176368426175 1103 34-35 0.2818603677020235 1103 36-37 0.20332262644389942 1103 38-39 0.23042935355680783 1103 40-41 0.26274790385235747 1103 42-43 0.44437216147616 1103 44-45 0.47486090113756774 1103 46-47 0.6870695836826215 1103 48-49 0.5465748870619436 1103 50-51 0.7228030756131218 1103 52-53 0.6031716901513491 1103 54-55 0.5014265874940875 1103 56-57 0.5350389145559404 1103 58-59 0.6761030364138279 1103 60-61 0.5627761378995686 1103 62-63 0.36584785737269243 1103 64-65 0.4737889569147633 1103 66-67 0.5875986954779435 1103 68-69 0.5243490755882227 1103 70-71 0.39066894400032837 1103 72-73 0.25516868954152727 1103 74-75 0.2188003405129102 1103 76-77 0.39801840947647094 1103 78-79 0.38632456845498453 1103 80-81 0.164918280696444 1103 82-83 0.02289730113838573 1103 84-85 0.02108781413363303 1103 86-87 -0.05077647728220924 1103 88-89 -0.029932655043943157 1103 90-91 -0.07143027168061522 1103 92-93 -0.24740827403292798 1103 94-95 -0.3616866786382218 1103 96-97 -0.6538495119263956 1103 98-99 -0.4480530728533587 1103 100-101 -0.7388330591601733 1104 1 0.18393964925153128 1104 2 0.17132702935148103 1104 3 0.22647226661618447 1104 4 0.19125497873429964 1104 5 0.22209204756639878 1104 6 0.18913629871119042 1104 7 0.24983986420059523 1104 8 0.2645494860437836 1104 9 0.45851792869965635 1104 10-11 0.3418491669847228 1104 12-13 0.3277920713733593 1104 14-15 0.4053774699311461 1104 16-17 0.32989187248308127 1104 18-19 0.3649096305838313 1104 20-21 0.3313174604739615 1104 22-23 0.47926060552541117 1104 24-25 0.513940004212941 1104 26-27 0.30950471904211696 1104 28-29 0.30560494714332975 1104 30-31 0.33711414353334845 1104 32-33 0.26563626945939234 1104 34-35 0.2219014687381744 1104 36-37 0.23405015066750678 1104 38-39 0.29390570607907307 1104 40-41 0.35125596073195453 1104 42-43 0.41937833432094607 1104 44-45 0.4882101941581638 1104 46-47 0.338418339587399 1104 48-49 0.36325691820270123 1104 50-51 0.4154075091823515 1104 52-53 0.4257199885678844 1104 54-55 0.548874048539659 1104 56-57 0.6405121133027407 1104 58-59 0.47135127021128653 1104 60-61 0.3587389122992519 1104 62-63 0.46279583773463884 1104 64-65 0.2715389371929824 1104 66-67 0.26282575221713955 1104 68-69 0.5515293855946126 1104 70-71 0.11106014999620228 1104 72-73 0.19676603096064937 1104 74-75 -0.056263758819909526 1104 76-77 0.07782789569358428 1104 78-79 -0.18835076269286688 1104 80-81 -0.2984897789456262 1104 82-83 -0.41873635113238095 1104 84-85 -0.2788036520188193 1104 86-87 -0.22532416510685138 1104 88-89 -0.4434480381718444 1104 90-91 -0.4527244018249732 1104 92-93 -0.35320456816164736 1104 94-95 -0.9710081156821815 1104 96-97 -1.2120257616675936 1104 98-99 -1.2974748189809837 1104 100-101 -1.3978780068682735 1105 1 0.25269223248063 1105 2 0.1733795192116716 1105 3 0.2780262694863609 1105 4 0.21131614959940492 1105 5 0.21336198670721274 1105 6 0.07417464230064752 1105 7 0.29277709562946796 1105 8 0.28923167263795335 1105 9 0.19321687833329548 1105 10-11 0.3079989393895346 1105 12-13 0.31474928835647376 1105 14-15 0.3629474661232095 1105 16-17 0.23528003102864403 1105 18-19 0.4520550760375528 1105 20-21 0.3612043201211108 1105 22-23 0.3744792771392298 1105 24-25 0.38771336846144777 1105 26-27 0.35683069579068416 1105 28-29 0.3307944968720946 1105 30-31 0.3407953720459673 1105 32-33 0.33552621881793954 1105 34-35 0.21144848723444198 1105 36-37 0.4559131111259731 1105 38-39 0.2952552865965501 1105 40-41 0.35589174744971075 1105 42-43 0.26243576636422716 1105 44-45 0.19863891871716532 1105 46-47 0.31339357446645266 1105 48-49 0.3283576793829184 1105 50-51 0.1412655477847764 1105 52-53 0.3011058298298934 1105 54-55 0.3247051876810261 1105 56-57 0.1697295354806414 1105 58-59 0.30129667175858543 1105 60-61 0.48048587306669077 1105 62-63 0.41051724333650697 1105 64-65 0.27279623290953126 1105 66-67 0.22802720004505517 1105 68-69 0.2574857529070904 1105 70-71 0.0589903821446498 1105 72-73 -0.1393014711025451 1105 74-75 -0.286518538047833 1105 76-77 -0.4718679439016107 1105 78-79 -0.43060798658717303 1105 80-81 -0.16046451106899795 1105 82-83 -0.36629717304981924 1105 84-85 -0.37929272080961596 1105 86-87 -0.44104131015931713 1105 88-89 -0.5715805380058399 1105 90-91 -0.5381856835402523 1105 92-93 -0.7506874019690564 1105 94-95 -0.7204994651678405 1105 96-97 -0.9529427554868128 1105 98-99 -1.1496199324262442 1105 100-101 -1.3362352832063706 1106 1 0.42082488686791564 1106 2 0.3764571822550735 1106 3 0.4790390124214383 1106 4 0.40097631310212023 1106 5 0.34600830789609915 1106 6 0.3901056264089533 1106 7 0.47632793965659914 1106 8 0.34394608062398646 1106 9 0.5803687852923929 1106 10-11 0.5588210088206793 1106 12-13 0.651257177444684 1106 14-15 0.5891446462764307 1106 16-17 0.14118769814989918 1106 18-19 0.6541839274108057 1106 20-21 0.6705718935262936 1106 22-23 0.7285917155045567 1106 24-25 0.5214199329177589 1106 26-27 0.5720382707493528 1106 28-29 0.5235149962529491 1106 30-31 0.4659617352382881 1106 32-33 0.49122304719983845 1106 34-35 0.5551653733038435 1106 36-37 0.5408953881246177 1106 38-39 0.573418726576584 1106 40-41 0.6182165152881041 1106 42-43 0.4656278484471912 1106 44-45 0.5552985997479567 1106 46-47 0.486375893289285 1106 48-49 0.5071014300270775 1106 50-51 0.47211238311246717 1106 52-53 0.5405989206227417 1106 54-55 0.4526301550805556 1106 56-57 0.3319498922111208 1106 58-59 0.5102468994763782 1106 60-61 0.8221756301727012 1106 62-63 0.5485987676490538 1106 64-65 0.3259840578872364 1106 66-67 0.2782266865037286 1106 68-69 0.2539433932160513 1106 70-71 0.11300333868184964 1106 72-73 0.11670873997518427 1106 74-75 0.17340910609999227 1106 76-77 -0.08007594158335607 1106 78-79 -0.17583995401210473 1106 80-81 -0.08292723899217336 1106 82-83 0.2597575061063502 1106 84-85 0.19213642676853837 1106 86-87 0.276083605258723 1106 88-89 -0.010290022686984202 1106 90-91 0.1009157535688594 1106 92-93 0.21548519153415668 1106 94-95 -0.14806265330649637 1106 96-97 -0.36621536407660926 1106 98-99 -0.5911713709543882 1106 100-101 -1.132498590394606 1107 1 -0.08559951230549956 1107 2 -0.00646928338608177 1107 3 0.034625028594930995 1107 4 0.049263121785649844 1107 5 0.09061714259178189 1107 6 0.006828014162181262 1107 7 0.07991782663435743 1107 8 0.03145290695768921 1107 9 -0.017892192544415764 1107 10-11 0.07805748066559914 1107 12-13 0.0716873839167036 1107 14-15 0.11190855779841513 1107 16-17 0.12372580001408551 1107 18-19 0.10632873415456601 1107 20-21 0.23408724240109535 1107 22-23 0.3167671948770163 1107 24-25 0.21288466753175328 1107 26-27 0.17213321139846727 1107 28-29 0.1496930849232001 1107 30-31 0.06358322213390721 1107 32-33 0.11815530921745676 1107 34-35 0.1369187744563547 1107 36-37 0.18527507645060126 1107 38-39 0.04932804883633679 1107 40-41 0.08186505958610013 1107 42-43 0.04411389323668402 1107 44-45 0.023024513166021165 1107 46-47 -0.12125136049305496 1107 48-49 -0.11801024879227384 1107 50-51 -0.2109103611392058 1107 52-53 -0.30208603479310625 1107 54-55 -0.2373292364255164 1107 56-57 -0.46747522126984364 1107 58-59 -0.5079052775233563 1107 60-61 -0.3450341851121763 1107 62-63 -0.42609642289849603 1107 64-65 -0.3849078763353013 1107 66-67 -0.35314596906022544 1107 68-69 -0.4297013665625329 1107 70-71 -0.5512468513632847 1107 72-73 -0.4963921412225787 1107 74-75 -0.5418460840980508 1107 76-77 -0.48201989028427406 1107 78-79 -0.5485966842310255 1107 80-81 -0.5033665927513553 1107 82-83 -0.47258522608729336 1107 84-85 -0.594908720964245 1107 86-87 -0.49620287248415096 1107 88-89 -0.42323433509496766 1107 90-91 -0.5445244739610331 1107 92-93 -0.7586473725896923 1107 94-95 -0.7540077619380412 1107 96-97 -0.6952827394441385 1107 98-99 -0.6817415281894306 1107 100-101 -0.7422470591114294 1108 1 -0.09118399835661606 1108 2 -0.04539112524470923 1108 3 -0.009161217714371617 1108 4 0.09582091152711314 1108 5 0.067206342865191 1108 6 0.02763110553635073 1108 7 0.037958429547451544 1108 8 -0.04503517359984244 1108 9 0.01039137955358882 1108 10-11 0.11571114211538713 1108 12-13 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-0.6954987644678852 2316 50-51 -0.8344805463934719 2316 52-53 -0.7597645816825178 2316 54-55 -0.6329813890001716 2316 56-57 -0.6423653676540866 2316 58-59 -0.3561140419278459 2316 60-61 -0.4646361845624938 2316 62-63 -0.5319090533649984 2316 64-65 -0.5707367599019229 2316 66-67 -0.5708154282826925 2316 68-69 -0.44269893882216493 2316 70-71 -0.407901583821058 2316 72-73 -0.37682756398313444 2316 74-75 -0.27448851753150194 2316 76-77 -0.47445508237677814 2316 78-79 -0.18997636548376917 2316 80-81 -0.27548864069104795 2316 82-83 -0.29521703406677346 2316 84-85 -0.2875517187982517 2316 86-87 -0.49638875658081716 2316 88-89 -0.5211451532630988 2316 90-91 -0.45417130644694836 2316 92-93 -0.31629066489356106 2316 94-95 -0.3227454105635239 2316 96-97 -0.4730408177338532 2316 98-99 -0.8071963205227455 2316 100-101 -0.5523154333760711 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6833.0 3 2176.0 4 2008.0 5 2180.0 6 2654.0 7 3304.0 8 4047.0 9 5057.0 10 6217.0 11 7681.0 12 9674.0 13 11744.0 14 13621.0 15 16450.0 16 19709.0 17 24943.0 18 24419.0 19 20594.0 20 21174.0 21 22796.0 22 24973.0 23 27690.0 24 31071.0 25 34864.0 26 39723.0 27 45212.0 28 51539.0 29 59428.0 30 69200.0 31 82592.0 32 100057.0 33 122968.0 34 157431.0 35 205198.0 36 254142.0 37 270224.0 38 140031.0 39 8146.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.945418927281 18.567754805911274 14.55780895803595 22.929017308771783 2 15.533748658855348 19.7990115642168 40.31789677761323 24.349342999314626 3 19.321898045067563 24.16399531395044 29.53124623158383 26.982860409398167 4 12.76792768389407 16.53748678958763 36.63819373903407 34.05639178748422 5 13.886160914819614 37.30790456511416 33.69226227909186 15.11367224097437 6 32.26002529940366 35.62929186312037 17.27403032642802 14.836652511047955 7 28.98230974118951 32.85661498668227 20.02956074579298 18.131514526335238 8 26.805769191328782 34.64088681064009 19.291259049611355 19.262084948419776 9 26.837073322878496 16.65422653958756 19.980331774341735 36.52836836319221 10-11 25.421069651160792 26.4868126516688 26.569873879769705 21.5222438174007 12-13 26.264725215685893 23.85978839101121 27.380678094995698 22.494808298307202 14-15 22.97522632879179 25.231536470425258 25.97377177027724 25.819465430505712 16-17 22.290116597045003 28.592647090512262 26.572310587520636 22.544925724922095 18-19 22.175481062134505 26.848311610622165 29.14064357780479 21.83556374943854 20-21 23.04059714939047 25.915884393372007 29.286136819201623 21.7573816380359 22-23 22.683833183471126 25.788457880677168 29.289355385452183 22.23835355039952 24-25 22.403084535116932 26.05228593053121 29.356741541302227 22.187887993049628 26-27 22.10908185949973 26.38430465028869 29.299602459389668 22.207011030821906 28-29 22.019266877756184 26.543540804370085 29.419182332504985 22.018009985368746 30-31 22.176283815075447 26.153778297936288 29.316510696044222 22.353427190944046 32-33 22.032561449013212 26.26654505029757 29.265520579449326 22.43537292123989 34-35 22.607194589941606 26.388137791046674 28.93367355180857 22.07099406720315 36-37 22.581521885445916 26.1367963121409 28.975405811418998 22.30627599099419 38-39 22.245680334085435 26.384813907000787 28.96609190732733 22.40341385158645 40-41 22.42273068776654 26.24727871704886 29.01561137836554 22.31437921681906 42-43 22.350138333451678 26.182076198879233 28.78668758736361 22.681097880305483 44-45 22.29304439731129 26.470547531643167 28.72331973670587 22.513088334339667 46-47 22.43488598126242 26.702437854097848 28.32949374901319 22.533182415626545 48-49 22.563300182349632 26.543289136293215 28.277519993746548 22.615890687610605 50-51 22.518420282328986 26.861444310076934 28.024486556897692 22.59564885069639 52-53 22.64717202472073 26.985240644780724 27.802209572696707 22.565377757801848 54-55 22.409963914055012 26.84239138653432 27.938605450851323 22.809039248559344 56-57 22.48944188636418 26.965554194302086 27.946322937211992 22.59868098212174 58-59 22.597513910785914 26.64037571489098 27.95721303949871 22.804897334824396 60-61 22.4933643904888 26.746253830996146 28.20094960268351 22.559432175831542 62-63 22.332545509210263 26.820169767084813 28.17376455960239 22.67352016410254 64-65 22.167199086621075 26.662361176782646 28.20946720844081 22.96097252815547 66-67 22.262837231938587 26.82142856227564 27.950171593646356 22.965562612139415 68-69 22.268652186362544 26.70407951649036 28.089809296491623 22.937459000655476 70-71 22.287430018244855 26.737349456883653 27.9374907404417 23.037729784429793 72-73 22.418510543462965 26.698666185486314 27.822306041470533 23.060517229580185 74-75 22.26144624384003 26.98697833104892 27.65984657353368 23.091728851577372 76-77 22.352753673246934 26.729338162154292 27.64314657781144 23.274761586787328 78-79 22.467617990944568 26.78035068658827 27.664908023563594 23.08712329890356 80-81 22.418003055995232 27.064878783895317 27.490862892002628 23.026255268106823 82-83 22.480895658766986 26.90194629439967 27.455852399666174 23.161305647167165 84-85 22.53648087364486 26.66792219805111 27.493549387223815 23.302047541080217 86-87 22.527164192566733 26.674078887623626 27.48377733203094 23.3149795877787 88-89 22.478443198658386 26.64281449781888 27.42097933002869 23.45776297349405 90-91 22.53765127446525 26.683061465878676 27.42523858715779 23.354048672498283 92-93 22.74021681693985 26.296393058965872 27.452163797985868 23.51122632610841 94-95 22.495197480770056 26.358286778016577 27.505302447829887 23.641213293383483 96-97 22.568960399009246 26.212001347394498 27.464903910981725 23.75413434261453 98-99 22.60129150530113 26.195544657872595 27.06747606398726 24.135687772839017 100-101 23.340025015570603 25.298868348120703 27.082433385013026 24.278673251295668 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1188.0 1 798.5 2 536.5 3 691.0 4 604.5 5 602.5 6 1111.0 7 2270.5 8 3560.5 9 4104.5 10 3425.5 11 2562.5 12 2471.0 13 2651.0 14 2862.0 15 3034.5 16 3107.0 17 3165.5 18 3247.5 19 3480.5 20 3760.0 21 4252.5 22 5137.0 23 6041.5 24 7134.5 25 8783.5 26 10722.0 27 12896.0 28 15251.0 29 17869.5 30 20782.5 31 23866.5 32 27594.0 33 30371.0 34 32941.5 35 36302.5 36 39802.5 37 43733.5 38 46802.5 39 48760.5 40 51058.5 41 54800.5 42 58994.0 43 63016.5 44 66490.0 45 69506.5 46 70644.0 47 72529.5 48 76423.5 49 77094.5 50 75071.0 51 72353.0 52 70118.0 53 69261.0 54 68855.0 55 65346.5 56 59067.5 57 53763.0 58 49305.5 59 43653.5 60 36637.0 61 30235.0 62 24745.0 63 19650.5 64 15643.0 65 12365.0 66 9297.5 67 7254.5 68 5587.0 69 3963.0 70 2935.0 71 2201.5 72 1696.5 73 1358.0 74 1044.5 75 828.0 76 550.0 77 299.0 78 169.0 79 95.5 80 55.5 81 27.5 82 16.0 83 19.5 84 14.5 85 9.5 86 8.0 87 6.0 88 4.5 89 3.0 90 2.0 91 2.5 92 3.0 93 2.0 94 1.0 95 1.5 96 1.0 97 0.5 98 1.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14433045680051604 2 0.05185034514807321 3 0.15411644091443105 4 0.13070180876752446 5 0.1970518057702466 6 0.19976728827306073 7 0.16605431682302893 8 0.07229331719756057 9 0.12470725305376501 10-11 0.1027015976771865 12-13 0.15309173053601063 14-15 0.13966802457870314 16-17 0.13654265792452086 18-19 0.1342626773325354 20-21 0.13546671202717941 22-23 0.19830707598381164 24-25 0.2176997198954181 26-27 0.14927468437639457 28-29 0.1290622721620518 30-31 0.09929443566893863 32-33 0.12665420277276382 34-35 0.09465762120658622 36-37 0.08692105784951205 38-39 0.05187596290753372 40-41 0.06017611697273911 42-43 0.0630453060323163 44-45 0.040834708580053704 46-47 0.05323370415894077 48-49 0.043473337804486284 50-51 0.05471953420765038 52-53 0.02735976710382519 54-55 0.0518247273886127 56-57 0.028000211090337953 58-59 0.05310561536163822 60-61 0.03814484383670011 62-63 0.05325932191840129 64-65 0.03963067388540971 66-67 0.040757855301672176 68-69 0.06570955301620938 70-71 0.05553930251038672 72-73 0.05187596290753372 74-75 0.044523665942367216 76-77 0.022133744173881056 78-79 0.023363396627985557 80-81 0.026873029674075493 82-83 0.02292789471715688 84-85 0.02080162068193451 86-87 0.025054168752379247 88-89 0.06581202405405143 90-91 0.11112984053969446 92-93 0.08200244803309405 94-95 0.07646901198962379 96-97 0.06865559535416808 98-99 0.05643592409150459 100-101 0.1324438164108392 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1951771.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.40880373368508 #Duplication Level Percentage of deduplicated Percentage of total 1 76.20746301056754 42.225643609946026 2 13.218897540090344 14.648865987491167 3 4.482500323725501 7.451099420204585 4 2.0328715196862515 4.505559162003745 5 1.1145584747645596 3.087817588897244 6 0.6630670190687096 2.204385019311465 7 0.44984174690692263 1.74476351459086 8 0.307115233390582 1.3613510152450916 9 0.2286349755515714 1.1401551438293616 >10 1.170922175577485 11.691435509475918 >50 0.07682222406448078 2.9166243509689562 >100 0.04368938229710386 4.331980741454281 >500 0.002689098845048495 1.0124276124925253 >1k 6.490928246668777E-4 0.503156742407475 >5k 2.7818263914294763E-4 1.1747345816812529 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9643 0.4940641089554051 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8097 0.4148539967035067 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5124 0.26253079895131143 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1965 0.10067779467980617 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 5.123551892102096E-5 0.0 0.0 0.0 0.0 8 5.123551892102096E-5 0.0 0.0 0.0 0.0 9 5.123551892102096E-5 0.0 0.0 0.0 0.0 10-11 5.123551892102096E-5 0.0 0.0 0.0 0.0 12-13 5.123551892102096E-5 0.0 0.0 5.123551892102096E-5 0.0 14-15 5.123551892102096E-5 0.0 0.0 5.123551892102096E-5 0.0 16-17 5.123551892102096E-5 0.0 0.0 5.123551892102096E-5 0.0 18-19 5.123551892102096E-5 0.0 0.0 1.5370655676306288E-4 0.0 20-21 5.123551892102096E-5 0.0 0.0 1.7932431622357336E-4 0.0 22-23 5.123551892102096E-5 0.0 0.0 3.330308729866362E-4 0.0 24-25 5.123551892102096E-5 0.0 0.0 7.172972648942934E-4 0.0 26-27 5.123551892102096E-5 0.0 0.0 0.0014089767703280762 0.0 28-29 7.685327838153144E-5 0.0 0.0 0.00532849396778618 0.0 30-31 1.0247103784204192E-4 0.0 0.0 0.017343223154765594 0.0 32-33 1.0247103784204192E-4 0.0 0.0 0.04042482442868554 0.0 34-35 1.0247103784204192E-4 0.0 0.0 0.07331802757598099 0.0 36-37 1.0247103784204192E-4 0.0 0.0 0.1324694341702997 0.0 38-39 1.5370655676306288E-4 0.0 0.0 0.2197747584117194 0.0 40-41 1.5370655676306288E-4 0.0 0.0 0.30818164630994105 0.0 42-43 1.5370655676306288E-4 0.0 0.0 0.3988685148001482 0.0 44-45 1.5370655676306288E-4 0.0 0.0 0.5053615408774903 0.0 46-47 2.0494207568408384E-4 0.0 0.0 0.6307604734366891 0.0 48-49 2.0494207568408384E-4 0.0 0.0 0.7602070119906486 0.0 50-51 2.0494207568408384E-4 0.0 0.0 0.8907294964419494 0.0 52-53 2.0494207568408384E-4 0.0 0.0 1.0197149153256198 0.0 54-55 2.0494207568408384E-4 0.0 0.0 1.1646345805937273 0.0 56-57 2.561775946051048E-4 0.0 0.0 1.3266925269409167 0.0 58-59 2.561775946051048E-4 0.0 0.0 1.4970506273533113 0.0 60-61 2.561775946051048E-4 0.0 0.0 1.6779120091445154 0.0 62-63 2.561775946051048E-4 0.0 0.0 1.8582098002275882 0.0 64-65 2.561775946051048E-4 0.0 0.0 2.04801178007051 0.0 66-67 2.561775946051048E-4 0.0 0.0 2.2515704967437262 0.0 68-69 2.561775946051048E-4 0.0 0.0 2.4735740002285107 0.0 70-71 2.817953540656153E-4 0.0 0.0 2.7144577924356903 0.0 72-73 3.0741311352612576E-4 0.0 0.0 2.954419345302292 0.0 74-75 3.0741311352612576E-4 0.0 0.0 3.1886681378092 0.0 76-77 3.0741311352612576E-4 0.0 0.0 3.439491620687058 0.0 78-79 3.0741311352612576E-4 0.0 0.0 3.7193912605525954 0.0 80-81 3.586486324471467E-4 0.0 0.0 4.016634123572899 0.0 82-83 4.098841513681677E-4 0.0 0.0 4.3270445149559045 0.0 84-85 4.098841513681677E-4 0.0 0.0 4.62892419243856 0.0 86-87 4.098841513681677E-4 0.0 0.0 4.931239371832044 0.0 88-89 4.098841513681677E-4 0.0 5.123551892102096E-5 5.256687388018369 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5775 0.0 41.97337 1 GTATCAA 9680 0.0 39.849026 1 TATCAAC 11985 0.0 31.904034 2 ATCAACG 11995 0.0 31.877436 3 GACCGTA 60 0.0039358176 31.692598 7 TCAACGC 12185 0.0 31.38037 4 CAACGCA 12320 0.0 31.075115 5 AACGCAG 12605 0.0 30.403984 6 ACGCAGA 14240 0.0 26.640478 7 CGCAGAG 14440 0.0 26.071287 8 GTACATG 9020 0.0 24.761353 1 GCAGAGT 15360 0.0 23.982403 9 TACATGG 9130 0.0 23.961535 2 GAGTACT 8235 0.0 22.827854 12-13 ACATGGG 9475 0.0 22.235765 3 AGTACTT 8465 0.0 21.6461 12-13 GTACTTT 8685 0.0 21.535332 14-15 AGAGTAC 13045 0.0 21.423912 10-11 TCGCGTA 135 3.780251E-4 21.125143 9 CATGGGG 4975 0.0 20.935272 4 >>END_MODULE