FastQCFastQC Report
Thu 26 May 2016
SRR938064_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938064_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1951771
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98960.5070266952424235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83860.4296610616716818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54320.27831133877898584No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21540.11036130775587914No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64700.037.1517331
GTATCAA100000.034.7256361
ATCAACG123800.027.7726443
TATCAAC124350.027.730452
TCAACGC124850.027.6144434
CAACGCA126250.027.2329925
AACGCAG128500.026.7191936
ACGCAGA144850.023.60497
CGCAGAG146350.023.2980678
GAGTACT76750.023.0808612-13
GTACATG87100.023.0703961
TACATGG87550.022.7312832
GTATAGG4450.022.4177571
GGACCGA5950.022.3475176
GTACTTT80450.021.9603114-15
GTACGGG1302.94736E-421.9177576
GCAGAGT156150.021.5621689
ACATGGG91250.021.1817043
AGTACTT79950.021.08780912-13
AGAGTAC128200.020.89330310-11
Produced by FastQC (version 0.11.5)