##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938064_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1951771 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.508665719492704 34.0 31.0 34.0 31.0 34.0 2 32.72041443386545 34.0 31.0 34.0 31.0 34.0 3 32.776078751042 34.0 31.0 34.0 31.0 34.0 4 36.23752684100747 37.0 35.0 37.0 35.0 37.0 5 36.21215962323449 37.0 37.0 37.0 35.0 37.0 6 36.24115636516784 37.0 37.0 37.0 35.0 37.0 7 36.19741352853383 37.0 37.0 37.0 35.0 37.0 8 36.18605410163385 37.0 37.0 37.0 35.0 37.0 9 37.86496212926619 39.0 38.0 39.0 35.0 39.0 10-11 37.841561074531796 39.0 38.0 39.0 35.0 39.0 12-13 37.76423566084341 39.0 38.0 39.0 35.0 39.0 14-15 39.034783793795484 40.0 38.0 41.0 35.5 41.0 16-17 38.92825182872376 40.0 38.0 41.0 35.5 41.0 18-19 39.17737403619584 40.0 39.0 41.0 36.0 41.0 20-21 39.162576705976264 40.0 39.0 41.0 36.0 41.0 22-23 39.070159870189684 40.0 39.0 41.0 35.5 41.0 24-25 38.98080743078978 40.0 38.0 41.0 35.0 41.0 26-27 38.81316865554412 40.0 38.0 41.0 35.0 41.0 28-29 38.699141702587035 40.0 38.0 41.0 34.5 41.0 30-31 38.465393224922394 40.0 38.0 41.0 34.0 41.0 32-33 38.260932763116166 40.0 38.0 41.0 34.0 41.0 34-35 38.120242077579796 40.0 38.0 41.0 34.0 41.0 36-37 38.1295728341081 40.0 38.0 41.0 34.0 41.0 38-39 37.98984665721542 40.0 37.5 41.0 33.0 41.0 40-41 37.82365169889295 40.0 37.0 41.0 33.0 41.0 42-43 37.594762141665186 40.0 37.0 41.0 33.0 41.0 44-45 37.31975651856698 40.0 37.0 41.0 32.5 41.0 46-47 36.912533027696384 40.0 36.0 41.0 31.0 41.0 48-49 36.51671123302887 39.0 36.0 41.0 30.5 41.0 50-51 36.14166185479752 39.0 35.0 41.0 29.5 41.0 52-53 35.64398154291666 39.0 35.0 40.0 28.0 41.0 54-55 35.34473075990985 38.0 35.0 40.0 27.5 41.0 56-57 35.00770633440091 38.0 34.0 40.0 27.0 41.0 58-59 34.58607080441302 38.0 34.0 40.0 26.0 41.0 60-61 34.11987471890913 37.0 33.0 40.0 25.0 41.0 62-63 33.67052666526965 36.5 33.0 39.5 23.5 41.0 64-65 33.19197436584517 36.0 32.5 39.0 22.5 40.0 66-67 32.6350053361793 35.5 31.5 39.0 21.5 40.0 68-69 32.075846244257136 35.0 31.0 38.0 19.5 40.0 70-71 31.49154255289171 35.0 30.5 37.5 17.0 40.0 72-73 30.910774880864608 34.5 30.0 37.0 12.0 39.0 74-75 30.311712285918787 34.0 29.0 36.0 8.0 39.0 76-77 28.95617262475977 32.5 28.0 35.0 7.0 37.0 78-79 29.64634170709576 34.0 29.0 35.0 6.0 37.0 80-81 29.3289107687326 34.0 29.0 35.0 2.0 37.0 82-83 28.77576211553507 34.0 28.5 35.0 2.0 36.5 84-85 28.191862416236333 33.5 27.0 35.0 2.0 36.0 86-87 27.550947062949497 33.0 26.0 35.0 2.0 35.5 88-89 26.826593642389398 32.5 25.0 35.0 2.0 35.0 90-91 26.29344093133877 32.0 24.5 34.5 2.0 35.0 92-93 25.479106411561602 32.0 21.5 34.0 2.0 35.0 94-95 24.658514754036204 31.0 18.5 34.0 2.0 35.0 96-97 23.607235428746506 31.0 4.5 34.0 2.0 35.0 98-99 22.18006671889274 30.5 2.0 34.0 2.0 35.0 100-101 17.796448712477027 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.081046353721419 1101 2 0.09882133151761252 1101 3 0.11186266632384445 1101 4 0.05431541192110956 1101 5 0.0622131855797079 1101 6 0.03275187569968807 1101 7 0.10835269156907401 1101 8 0.03966905041728097 1101 9 -0.01385760001291203 1101 10-11 0.0518088440154898 1101 12-13 0.14778146853250718 1101 14-15 0.15078095623854182 1101 16-17 0.18265092576912423 1101 18-19 0.23997257612240475 1101 20-21 0.17119305398788498 1101 22-23 0.20446083234033097 1101 24-25 0.24692533553988483 1101 26-27 0.25221027386783845 1101 28-29 0.20097146643054487 1101 30-31 0.3100987950053167 1101 32-33 0.3225090266507351 1101 34-35 0.3851881818355878 1101 36-37 0.4200655733143748 1101 38-39 0.36945984044854896 1101 40-41 0.45819145924610893 1101 42-43 0.5506652578651483 1101 44-45 0.5978843727002499 1101 46-47 0.46520164662818786 1101 48-49 0.4621918963092355 1101 50-51 0.31636479542992646 1101 52-53 0.08777894738840786 1101 54-55 0.1839272657563953 1101 56-57 0.3090110546576952 1101 58-59 0.3480699425939022 1101 60-61 0.4174453386772825 1101 62-63 0.43036231048511553 1101 64-65 0.3347954012946772 1101 66-67 0.04910619447303333 1101 68-69 0.3864066281790528 1101 70-71 0.2585054791695427 1101 72-73 0.23931818027097407 1101 74-75 0.3111325250330701 1101 76-77 0.5062994049184191 1101 78-79 0.4749011080510783 1101 80-81 0.3174522421219912 1101 82-83 0.40528229433770946 1101 84-85 0.4014166092759126 1101 86-87 0.1292180177514055 1101 88-89 -0.11928341206129289 1101 90-91 -0.19928008691842436 1101 92-93 -0.44298705077364176 1101 94-95 -0.739419273786492 1101 96-97 -0.3018324649768047 1101 98-99 -0.32542094347463646 1101 100-101 -0.6978415132435423 1102 1 0.06437968705475328 1102 2 0.08134514104142454 1102 3 0.08257695203813142 1102 4 0.05206144366714227 1102 5 0.058816360182881056 1102 6 -0.047216378268565506 1102 7 -0.06250445128807058 1102 8 0.09763730438553608 1102 9 0.06588843173312142 1102 10-11 0.13098344719009702 1102 12-13 0.11288464313568625 1102 14-15 0.15099524195283465 1102 16-17 0.054682671800861726 1102 18-19 0.15198844913827259 1102 20-21 0.08345495874979036 1102 22-23 0.08441321329270579 1102 24-25 0.1691396212541747 1102 26-27 0.18990075005832097 1102 28-29 0.02599527595435802 1102 30-31 0.12233689024340322 1102 32-33 0.04481855046025629 1102 34-35 0.12345008659749368 1102 36-37 0.20785922410802016 1102 38-39 0.1020074594961713 1102 40-41 0.25219939575404027 1102 42-43 0.1675938292937218 1102 44-45 0.07820183301771522 1102 46-47 0.14266990059643803 1102 48-49 0.36593792805527414 1102 50-51 0.30796003352516266 1102 52-53 0.29331862992808766 1102 54-55 0.38045107528020594 1102 56-57 0.35303486418150953 1102 58-59 0.3488794664034245 1102 60-61 0.1970247037566466 1102 62-63 0.17423532635813643 1102 64-65 0.21940651240579 1102 66-67 0.14247127383811886 1102 68-69 0.14263678690921466 1102 70-71 0.17075151091557217 1102 72-73 0.20881024376303614 1102 74-75 -0.024986522585979998 1102 76-77 0.016156547775562302 1102 78-79 0.16529793344790633 1102 80-81 0.01874589291564277 1102 82-83 -0.014106594551176244 1102 84-85 -0.19555164469234398 1102 86-87 -0.3045756330422478 1102 88-89 -0.40957706285494666 1102 90-91 -0.6196531027914389 1102 92-93 -0.5553521301387185 1102 94-95 -0.6903399087071236 1102 96-97 -0.7714038935482357 1102 98-99 -0.800770149823844 1102 100-101 -1.521778021180051 1103 1 0.09801015707095928 1103 2 0.0778902517923683 1103 3 0.04685842151408792 1103 4 0.08704613662590077 1103 5 0.057074959138404324 1103 6 0.064537628574584 1103 7 0.04871110786913846 1103 8 0.03505131483034063 1103 9 0.13428749530236672 1103 10-11 0.03296435840925227 1103 12-13 0.1239808974126575 1103 14-15 0.1654050204509403 1103 16-17 0.18140156249057782 1103 18-19 0.15803292959928683 1103 20-21 0.17459893497885304 1103 22-23 0.26391348341697096 1103 24-25 0.17115069634871816 1103 26-27 0.15934834595243075 1103 28-29 0.21916518411211428 1103 30-31 0.18922235755836425 1103 32-33 0.26859623819661493 1103 34-35 0.19702518114098666 1103 36-37 0.19612592679125385 1103 38-39 0.12785159779978983 1103 40-41 0.26565383479600513 1103 42-43 0.1966794586832421 1103 44-45 0.156336174814939 1103 46-47 0.050817298399429944 1103 48-49 -0.0045879138319975254 1103 50-51 -0.06167394810638882 1103 52-53 -0.02882165460280106 1103 54-55 -0.1678120257462652 1103 56-57 -0.03856832328472848 1103 58-59 0.06089729460439486 1103 60-61 0.025716852144903157 1103 62-63 -0.014644558388845041 1103 64-65 -0.07037693798138633 1103 66-67 -0.14463479495352516 1103 68-69 -0.3229759449474443 1103 70-71 -0.31454877721697017 1103 72-73 -0.41683895486832867 1103 74-75 -0.25111429135961316 1103 76-77 -0.12366246897213884 1103 78-79 -0.27105628949464844 1103 80-81 -0.27712705864207265 1103 82-83 -0.18172905088109204 1103 84-85 -0.33562097658501244 1103 86-87 -0.4995900396692967 1103 88-89 -0.5227721826100336 1103 90-91 -0.3978464217181461 1103 92-93 -0.39661721207641065 1103 94-95 -0.3916923308212965 1103 96-97 -0.4200829859307298 1103 98-99 -0.5324710322297506 1103 100-101 -0.9289993429880852 1104 1 0.0677622208800912 1104 2 0.10204536947227894 1104 3 0.09106140116833217 1104 4 0.10214461592743618 1104 5 0.03348572116220794 1104 6 0.04593479610031892 1104 7 0.023642095891688086 1104 8 0.06746451694337452 1104 9 0.07113765565393493 1104 10-11 0.18321211233388368 1104 12-13 0.16403660822676613 1104 14-15 0.12746203162073044 1104 16-17 0.11957870647549385 1104 18-19 0.16546967680769598 1104 20-21 0.22666485156300809 1104 22-23 0.08932535527127072 1104 24-25 0.13340293174442053 1104 26-27 0.1387405848852339 1104 28-29 -0.05872489624736943 1104 30-31 -0.048633940893481054 1104 32-33 -0.035270625431500946 1104 34-35 0.08851554082346524 1104 36-37 -0.06844576037039474 1104 38-39 0.10004602916757932 1104 40-41 0.009108696989919451 1104 42-43 0.05115339703225885 1104 44-45 -0.10424530573938995 1104 46-47 -0.12701659269706056 1104 48-49 -0.0764406814451064 1104 50-51 0.07629732046945747 1104 52-53 -0.005670709965308163 1104 54-55 -0.15218554394312633 1104 56-57 -0.06115552415094072 1104 58-59 -0.01841187079565998 1104 60-61 -0.12053357539757314 1104 62-63 -3.8834845003776763E-4 1104 64-65 -0.005414403660516598 1104 66-67 -0.2825448334658134 1104 68-69 -0.18495341399279752 1104 70-71 -0.2625509256802232 1104 72-73 -0.45050153506903357 1104 74-75 -0.4885364259421543 1104 76-77 -0.579697432192809 1104 78-79 -0.6235543479320533 1104 80-81 -0.7030242997862821 1104 82-83 -0.87169503829276 1104 84-85 -0.7251600907768037 1104 86-87 -0.8748336427652035 1104 88-89 -0.9135290630892356 1104 90-91 -0.9224767131704006 1104 92-93 -0.8002912152438562 1104 94-95 -0.5925252305603443 1104 96-97 -0.42645660836109656 1104 98-99 -0.6255833050982567 1104 100-101 -1.6204308648513468 1105 1 0.14634198354146832 1105 2 0.17147796023593997 1105 3 0.09624019111267756 1105 4 0.08890520259316048 1105 5 0.10743389802921399 1105 6 0.10813637808676191 1105 7 0.08901225822349801 1105 8 0.13043364828727277 1105 9 0.11340057112186486 1105 10-11 0.14312283593616115 1105 12-13 0.16288392905399718 1105 14-15 0.2351276326983367 1105 16-17 0.11569095514848016 1105 18-19 0.16652858052930952 1105 20-21 0.12311005729306856 1105 22-23 0.15205245922244615 1105 24-25 0.1510869220253852 1105 26-27 0.18035276376868836 1105 28-29 0.16817822368357582 1105 30-31 0.13406353977211438 1105 32-33 0.1719500843077313 1105 34-35 0.15313089208163433 1105 36-37 0.15140821011202377 1105 38-39 0.15263942179264944 1105 40-41 0.11394246916164263 1105 42-43 -0.09008611929240118 1105 44-45 -0.049144639418734926 1105 46-47 -0.052882084550660124 1105 48-49 0.011522046878468473 1105 50-51 -0.15486987221605375 1105 52-53 -0.191018702262717 1105 54-55 -0.03625415179777036 1105 56-57 0.037415755355461044 1105 58-59 0.008061128785605831 1105 60-61 -0.05898286550927878 1105 62-63 -0.11018341114543517 1105 64-65 -0.22275215654593694 1105 66-67 -0.32036791496508243 1105 68-69 -0.3311585572781617 1105 70-71 -0.3524368638344981 1105 72-73 -0.44040712270368587 1105 74-75 -0.5369938062192254 1105 76-77 -0.18331645993652046 1105 78-79 -0.3799391416734892 1105 80-81 -0.3154878974299713 1105 82-83 -0.23909302699905055 1105 84-85 -0.5442477315246776 1105 86-87 -0.7301554673752975 1105 88-89 -0.8229396735376078 1105 90-91 -0.8826572444652498 1105 92-93 -0.7279499593303775 1105 94-95 -0.8013281977673934 1105 96-97 -1.0037817855790827 1105 98-99 -1.111308567418817 1105 100-101 -1.5283475727367524 1106 1 0.16146471513919636 1106 2 0.20982954933117526 1106 3 0.15889014204393703 1106 4 0.1570075344412416 1106 5 0.17911399075941858 1106 6 0.1432032743881564 1106 7 0.21701908154167882 1106 8 0.20513165521515475 1106 9 0.11874271541832115 1106 10-11 0.19351814923670219 1106 12-13 0.20144926964481868 1106 14-15 0.2733063982573114 1106 16-17 0.3347013947655455 1106 18-19 0.3238746264253791 1106 20-21 0.3735861809074521 1106 22-23 0.37974828979861286 1106 24-25 0.41815037081838113 1106 26-27 0.37662697512126186 1106 28-29 0.4638216365973946 1106 30-31 0.2695313508283306 1106 32-33 0.3667268402142767 1106 34-35 0.4153709659312099 1106 36-37 0.4255934225714526 1106 38-39 0.3478214242453461 1106 40-41 0.35688740014425946 1106 42-43 0.32344072063596485 1106 44-45 0.04966157864363652 1106 46-47 0.001763612262784875 1106 48-49 0.05246449529654029 1106 50-51 0.07103334361619318 1106 52-53 -0.25745837310632425 1106 54-55 -0.2892864804044706 1106 56-57 -0.25996097157289455 1106 58-59 -0.15271166278503046 1106 60-61 -0.25909205652484246 1106 62-63 -0.24316089031176347 1106 64-65 -0.2365898398441928 1106 66-67 -0.476380814740871 1106 68-69 -0.6897841247054828 1106 70-71 -1.0439552164393255 1106 72-73 -0.5777473132128783 1106 74-75 -0.38554482202428986 1106 76-77 -0.47260289440921355 1106 78-79 -0.36811126017337514 1106 80-81 -0.5224783630079166 1106 82-83 -0.5679995185617663 1106 84-85 -0.43446087059459515 1106 86-87 -0.4906363858345557 1106 88-89 -0.6319408371463773 1106 90-91 -0.663603486935024 1106 92-93 -0.5760497219780909 1106 94-95 -0.8090002483029686 1106 96-97 -0.6244282979642719 1106 98-99 -0.6207233424121306 1106 100-101 -1.2449944973889764 1107 1 0.012531316127429193 1107 2 -0.007107239910958185 1107 3 0.0643814633163231 1107 4 0.004582329214343872 1107 5 0.018649275805273646 1107 6 0.03490977043653487 1107 7 -0.02346309790461021 1107 8 -0.0031542578533958476 1107 9 0.001398039084833158 1107 10-11 0.06056260341398456 1107 12-13 0.010029588833262437 1107 14-15 0.05963245999794964 1107 16-17 0.010915963363103742 1107 18-19 0.016491373114888574 1107 20-21 -0.0444930362376752 1107 22-23 0.03576346391928098 1107 24-25 0.13576556110379556 1107 26-27 0.10152982274001943 1107 28-29 0.06720454913731544 1107 30-31 0.0649465393662112 1107 32-33 0.062221432665772625 1107 34-35 0.14843442243709148 1107 36-37 0.13893587406624874 1107 38-39 0.15410645698990066 1107 40-41 -0.04901718737043126 1107 42-43 -0.003364817322825786 1107 44-45 -0.07272652955538206 1107 46-47 0.00988585715450796 1107 48-49 -0.11166336684865286 1107 50-51 -0.08880061810391027 1107 52-53 0.03854210528314894 1107 54-55 -0.01689228311578006 1107 56-57 -0.08396074985358126 1107 58-59 -0.0024864483835429496 1107 60-61 -0.015780225232497003 1107 62-63 -0.015086937635182096 1107 64-65 -0.10786061374291478 1107 66-67 -0.32989192582771665 1107 68-69 -0.022136604903650436 1107 70-71 -0.08024264113121049 1107 72-73 -0.2221536242403026 1107 74-75 -0.012846798275202076 1107 76-77 -0.250779542450001 1107 78-79 -0.2866026513474189 1107 80-81 -0.21119249472011248 1107 82-83 -0.21949402145176222 1107 84-85 -0.31055895463386207 1107 86-87 -0.4648910048049899 1107 88-89 -0.7727683744673115 1107 90-91 -0.8416184327830862 1107 92-93 -0.8098310357360461 1107 94-95 -1.024808371530849 1107 96-97 -1.2275787055783063 1107 98-99 -1.6037066577603518 1107 100-101 -2.071078355348808 1108 1 -0.056441931189546324 1108 2 0.024598811769322992 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2 1.0 3 7.0 4 9.0 5 37.0 6 64.0 7 164.0 8 355.0 9 782.0 10 1313.0 11 2252.0 12 3549.0 13 5487.0 14 7376.0 15 10286.0 16 13848.0 17 19809.0 18 20470.0 19 15409.0 20 14242.0 21 14918.0 22 16406.0 23 17838.0 24 20368.0 25 23682.0 26 26872.0 27 32032.0 28 38341.0 29 46369.0 30 56251.0 31 68929.0 32 86410.0 33 110958.0 34 149557.0 35 211500.0 36 301138.0 37 383697.0 38 219874.0 39 11171.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.286791866951766 19.024202279881102 14.894601487092851 22.79440436607428 2 15.769240623734929 20.102640573516375 39.87870682101185 24.249411981736845 3 19.312264743589086 24.09712265973353 29.778017354019333 26.812595242658055 4 12.99606305667477 16.920197482487673 36.33392903254895 33.749810428288605 5 13.825788628016788 37.32405479407135 33.83937647560336 15.0107801023085 6 32.429251627137 35.85721671061888 16.98186388460354 14.731667777640578 7 29.014008303228195 32.91298005759897 19.91186466035206 18.161146978820774 8 26.80119747654822 34.907527573675395 19.119712302314156 19.171562647462228 9 26.62602323735725 16.721070248507637 19.96207546889466 36.69083104524045 10-11 25.46779309662865 26.58301101922305 26.447621160474256 21.50157472367404 12-13 26.204328213830447 23.831741942623857 27.422823186552392 22.541106656993307 14-15 22.90084236316658 25.298049822443307 25.914489968341574 25.886617846048537 16-17 22.28054723378048 28.635753215006144 26.57489059577078 22.508808955442593 18-19 22.12057664551835 26.8884003297518 29.15541833545021 21.835604689279634 20-21 22.916546049715876 25.91584770959298 29.39988861398187 21.76771762670928 22-23 22.645228359269606 25.805255842001955 29.365637669583162 22.183878129145274 24-25 22.430066718775098 26.033234112116133 29.411008200801405 22.125690968307367 26-27 22.106977976448835 26.32953795996845 29.443822956824572 22.119661106758134 28-29 21.909314846340965 26.578612630130777 29.556898868517163 21.9551736550111 30-31 22.158121475512143 26.19605861960484 29.371364070949358 22.274455833933665 32-33 21.99607433454027 26.27921000978086 29.328363829568122 22.396351826110745 34-35 22.56808052527534 26.356870770747488 29.059365049759528 22.015683654217646 36-37 22.565315675352196 26.092275043140727 29.14899024056335 22.193419040943727 38-39 22.212282213250596 26.373892412724544 29.119922365455704 22.293903008569156 40-41 22.406512612845948 26.184025837716007 29.0868236512349 22.322637898203148 42-43 22.30986358587163 26.214502813951295 28.86111316060222 22.614520439574854 44-45 22.27526808999011 26.484140036992066 28.797655461452937 22.442936411564887 46-47 22.43062886877093 26.699402823032365 28.409366591751628 22.460601716445076 48-49 22.512917190873157 26.545996061502454 28.367250165811924 22.57383658181246 50-51 22.48976075245884 26.85489410029249 28.119009172347027 22.536335974901643 52-53 22.698904439068805 26.96752362032647 27.826458557401274 22.507113383203446 54-55 22.406632838411927 26.818268692262798 27.963059463841123 22.812039005484152 56-57 22.4883727127194 26.983505547686736 27.96826350128418 22.559858238309683 58-59 22.604298388904905 26.687293808654434 27.96315300598956 22.745254796451107 60-61 22.47598952810639 26.821687036509957 28.156215566886416 22.54610786849724 62-63 22.341332149892487 26.870017608123923 28.13179650010352 22.656853741880074 64-65 22.1243244115818 26.752750461282147 28.11439438605338 23.00853074108267 66-67 22.16198115204065 26.954780167777688 27.90035516069343 22.982883519488233 68-69 22.211880859298923 26.74310619463584 28.081532535317233 22.963480410748 70-71 22.287040038125447 26.79921750593468 27.845156364297875 23.068586091641997 72-73 22.313130262488425 26.785460839374746 27.724324897638787 23.177084000498034 74-75 22.223511856263368 27.066480549414845 27.460877969190218 23.249129625131566 76-77 22.234051575810827 26.838582619664585 27.68775938633584 23.239606418188746 78-79 22.28166253855848 26.848641670471906 27.753801780083297 23.115894010886315 80-81 22.202639402051865 27.160491929324554 27.56612020093869 23.070748467684886 82-83 22.281906747605955 26.962644508818652 27.571893073212333 23.183555670363056 84-85 22.38677815210629 26.77861845105891 27.496558785576724 23.338044611258074 86-87 22.347423807679977 26.81752358655562 27.487021212204297 23.348031393560102 88-89 22.194585857674802 26.877254227108228 27.350212726268285 23.577947188948688 90-91 22.246918869719234 26.881990455261086 27.505391465494622 23.365699209525058 92-93 22.31383082991232 26.55016805493168 27.492105889596996 23.643895225559007 94-95 22.097430767859027 26.568964651407807 27.570077653962795 23.763526926770375 96-97 22.129790642814264 26.48280901585392 27.562736568034357 23.82466377329746 98-99 22.10520058917618 26.517770466736884 27.1830404575672 24.19398848651974 100-101 22.441329493319557 26.06987093116235 27.126438371852608 24.362361203665486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1260.0 1 825.5 2 531.0 3 675.5 4 552.5 5 537.5 6 1054.5 7 2316.5 8 3702.5 9 4194.0 10 3435.0 11 2547.0 12 2468.5 13 2572.0 14 2759.0 15 2960.5 16 3042.5 17 3068.5 18 3196.0 19 3405.0 20 3638.0 21 4160.0 22 4866.0 23 5766.0 24 6889.0 25 8312.5 26 10233.5 27 12600.0 28 14972.0 29 17570.5 30 20605.5 31 23835.0 32 27615.5 33 30429.5 34 33060.5 35 36401.0 36 40093.0 37 44011.5 38 46874.5 39 48993.0 40 51416.5 41 55265.5 42 59304.5 43 63164.0 44 67023.0 45 70189.5 46 70805.5 47 73278.0 48 78428.0 49 78663.5 50 76015.0 51 73169.5 52 70374.0 53 70034.0 54 70838.0 55 67062.0 56 59979.5 57 54738.0 58 50070.0 59 43350.0 60 35637.5 61 29258.0 62 23833.0 63 18475.0 64 14323.0 65 11014.0 66 7902.5 67 6196.5 68 4748.0 69 3216.0 70 2289.0 71 1671.5 72 1321.0 73 1069.5 74 824.0 75 611.5 76 389.5 77 197.0 78 103.5 79 60.0 80 30.5 81 15.0 82 5.0 83 2.0 84 2.5 85 2.5 86 1.5 87 1.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00911992236794173 2 0.015421891195227308 3 0.009068686849020709 4 1.5370655676306288E-4 5 0.001383359010867566 6 0.001537065567630629 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 9.734748594993982E-4 14-15 0.0 16-17 0.0032790732109453415 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0036633396028529983 26-27 0.018444786811567544 28-29 0.006583764181351193 30-31 0.0035352508055504464 32-33 0.0 34-35 0.009478571000388878 36-37 9.734748594993982E-4 38-39 0.00320221993256381 40-41 0.0025873937055115584 42-43 0.0034327797677084046 44-45 5.123551892102096E-5 46-47 2.305598351445943E-4 48-49 5.892084675917411E-4 50-51 0.004585578943431375 52-53 0.008607567178731521 54-55 0.007582856800311103 56-57 0.016754014687173854 58-59 0.02349148542528811 60-61 0.0036121040839319773 62-63 0.020442972049487362 64-65 0.021339593630605228 66-67 0.01332123491946545 68-69 0.030690075833691554 70-71 0.016318512776345176 72-73 0.00532849396778618 74-75 0.01603671742227956 76-77 0.021032180517079103 78-79 0.026335056725404773 80-81 0.011809787111295331 82-83 0.03478891734737323 84-85 0.021775095541433906 86-87 0.03107434222559921 88-89 0.02238992176848616 90-91 0.027615944698430296 92-93 0.024311253728024448 94-95 0.015012007043859141 96-97 0.015242566879003735 98-99 0.011912258149137373 100-101 0.011041254327480017 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1951771.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.8932199837827 #Duplication Level Percentage of deduplicated Percentage of total 1 74.11249203187789 39.20048344588459 2 14.181361972285073 15.001957969394494 3 4.928203526297616 7.820056597239404 4 2.274264115307976 4.811726086088309 5 1.2377309850052653 3.273378863531377 6 0.758176200728846 2.406142834297166 7 0.49138423389083674 1.8193626065825388 8 0.32824065295518673 1.3889364051503332 9 0.240695420312752 1.1458040234122289 >10 1.305451090064123 12.360245105213274 >50 0.08938387922964383 3.2123072227756024 >100 0.04854726241416312 4.687100979702544 >500 0.0030999082900105357 1.140524248264382 >1k 6.78104938439804E-4 0.5168969233339807 >5k 2.906164021884874E-4 1.2150766891298592 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9896 0.5070266952424235 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8386 0.4296610616716818 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5432 0.27831133877898584 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2154 0.11036130775587914 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 5.123551892102096E-5 0.0 0.0 5.123551892102096E-5 0.0 14-15 5.123551892102096E-5 0.0 0.0 5.123551892102096E-5 0.0 16-17 7.685327838153144E-5 0.0 0.0 5.123551892102096E-5 0.0 18-19 1.0247103784204192E-4 0.0 0.0 1.5370655676306288E-4 0.0 20-21 1.0247103784204192E-4 0.0 0.0 1.7932431622357336E-4 0.0 22-23 1.0247103784204192E-4 0.0 0.0 2.817953540656153E-4 0.0 24-25 1.5370655676306288E-4 0.0 0.0 6.660617459732724E-4 0.0 26-27 1.5370655676306288E-4 0.0 0.0 0.0013577412514070553 0.0 28-29 1.5370655676306288E-4 0.0 0.0 0.005251640689404648 0.0 30-31 1.5370655676306288E-4 0.0 0.0 0.017420076433147125 0.0 32-33 1.5370655676306288E-4 0.0 0.0 0.04073223754221166 0.0 34-35 1.7932431622357336E-4 0.0 0.0 0.07390723604357273 0.0 36-37 2.561775946051048E-4 0.0 0.0 0.13375032214332522 0.0 38-39 2.561775946051048E-4 0.0 0.0 0.22167047261179718 0.0 40-41 2.561775946051048E-4 0.0 0.0 0.310384773623545 0.0 42-43 2.561775946051048E-4 0.0 0.0 0.4014302907461992 0.0 44-45 2.561775946051048E-4 0.0 0.0 0.5090761159992643 0.0 46-47 2.817953540656153E-4 0.0 0.0 0.635474141177423 0.0 48-49 3.0741311352612576E-4 0.0 0.0 0.7656635947557373 0.0 50-51 3.0741311352612576E-4 0.0 0.0 0.8966215811178668 0.0 52-53 3.0741311352612576E-4 0.0 0.0 1.0270415945313256 0.0 54-55 3.0741311352612576E-4 0.0 0.0 1.1730884412156959 0.0 56-57 3.0741311352612576E-4 0.0 0.0 1.3373238971170287 0.0 58-59 3.0741311352612576E-4 0.0 0.0 1.508066263921331 0.0 60-61 3.0741311352612576E-4 0.0 0.0 1.6907721243936917 0.0 62-63 3.0741311352612576E-4 0.0 0.0 1.8727606876011582 5.123551892102096E-5 64-65 3.0741311352612576E-4 0.0 0.0 2.0626651384819223 1.0247103784204192E-4 66-67 3.330308729866362E-4 0.0 0.0 2.268631924544427 1.0247103784204192E-4 68-69 3.586486324471467E-4 5.123551892102096E-5 0.0 2.4930434974184985 1.0247103784204192E-4 70-71 3.586486324471467E-4 5.123551892102096E-5 0.0 2.734721440168954 1.0247103784204192E-4 72-73 3.586486324471467E-4 5.123551892102096E-5 0.0 2.977296004500528 1.0247103784204192E-4 74-75 3.586486324471467E-4 5.123551892102096E-5 0.0 3.214311515029171 1.0247103784204192E-4 76-77 3.586486324471467E-4 5.123551892102096E-5 0.0 3.4677736271314616 1.0247103784204192E-4 78-79 3.586486324471467E-4 5.123551892102096E-5 0.0 3.75123413556201 1.0247103784204192E-4 80-81 4.098841513681677E-4 5.123551892102096E-5 0.0 4.053907963587942 1.0247103784204192E-4 82-83 4.098841513681677E-4 5.123551892102096E-5 0.0 4.369211347027904 1.0247103784204192E-4 84-85 4.098841513681677E-4 5.123551892102096E-5 0.0 4.677495464375688 1.0247103784204192E-4 86-87 4.098841513681677E-4 5.123551892102096E-5 0.0 4.985830817242391 1.0247103784204192E-4 88-89 4.098841513681677E-4 5.123551892102096E-5 0.0 5.318912925747949 1.0247103784204192E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6470 0.0 37.151733 1 GTATCAA 10000 0.0 34.725636 1 ATCAACG 12380 0.0 27.772644 3 TATCAAC 12435 0.0 27.73045 2 TCAACGC 12485 0.0 27.614443 4 CAACGCA 12625 0.0 27.232992 5 AACGCAG 12850 0.0 26.719193 6 ACGCAGA 14485 0.0 23.6049 7 CGCAGAG 14635 0.0 23.298067 8 GAGTACT 7675 0.0 23.08086 12-13 GTACATG 8710 0.0 23.070396 1 TACATGG 8755 0.0 22.731283 2 GTATAGG 445 0.0 22.417757 1 GGACCGA 595 0.0 22.347517 6 GTACTTT 8045 0.0 21.96031 14-15 GTACGGG 130 2.94736E-4 21.917757 6 GCAGAGT 15615 0.0 21.562168 9 ACATGGG 9125 0.0 21.181704 3 AGTACTT 7995 0.0 21.087809 12-13 AGAGTAC 12820 0.0 20.893303 10-11 >>END_MODULE