FastQCFastQC Report
Thu 26 May 2016
SRR938063_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938063_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334381
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49820.3733566350240299No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44090.3304153761182151No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26280.1969452502696007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23900.056.9280171
GTATCAA41300.039.994951
TGGTATC10450.033.2128032
TATCAAC52350.031.2421382
GTGGTAT11150.030.827391
ATCAACG52900.030.5578123
TCAACGC53150.030.3246234
CAACGCA53700.029.9254995
AACGCAG56350.028.5950416
ACGCAGA64950.024.5800137
CGCAGAG65100.024.523388
GTATAGG3550.022.8612351
GTACCTG10550.022.625421
GCAGAGT71550.021.7133879
CAGAGTA65600.021.6549310-11
GTACATG40850.021.2695581
GAGTACT46450.020.81304212-13
TACATGG40050.020.5372622
TAAGGTG7100.020.0891655
AGAGTAC63400.020.00835810-11
Produced by FastQC (version 0.11.5)