FastQCFastQC Report
Thu 26 May 2016
SRR938063_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938063_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1334381
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50550.37882733642040767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47050.3525979461638018No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27430.20556347849677117No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.047.381781
GTATCAA44700.040.2255631
ATCAACG54700.032.4271663
TCAACGC55100.032.278064
TATCAAC55950.031.7972342
CAACGCA56250.031.7026985
AACGCAG57550.030.9482466
TGGTATC12850.027.7636132
ACGCAGA65650.026.985117
CGCAGAG66700.026.5603088
GTGGTAT14350.025.192981
GCAGAGT73500.023.9091689
GAGTACT48750.021.33632312-13
GTACCTG9300.020.9709821
AGAGTAC65600.020.9601710-11
GTACTTT49900.020.74942614-15
ACCGTGC2751.2605597E-920.7251978
GGACCGA3900.020.7030546
GTACATG38600.020.5800761
CGTACAC1856.4892392E-620.5639113
Produced by FastQC (version 0.11.5)