##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938063_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1334381 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44783086689634 33.0 31.0 34.0 31.0 34.0 2 32.68088424520433 34.0 31.0 34.0 31.0 34.0 3 32.744658384674246 34.0 31.0 34.0 31.0 34.0 4 36.21481196150125 37.0 35.0 37.0 35.0 37.0 5 36.13740453438711 37.0 35.0 37.0 35.0 37.0 6 36.185363850354584 37.0 37.0 37.0 35.0 37.0 7 36.154388439283835 37.0 36.0 37.0 35.0 37.0 8 36.14356394463051 37.0 36.0 37.0 35.0 37.0 9 37.83008975697346 39.0 38.0 39.0 35.0 39.0 10-11 37.79770395411805 39.0 38.0 39.0 35.0 39.0 12-13 37.71920013849118 39.0 38.0 39.0 35.0 39.0 14-15 38.96672839316507 40.0 38.0 41.0 35.5 41.0 16-17 38.87011168474371 40.0 38.0 41.0 35.0 41.0 18-19 39.110345171281665 40.0 38.5 41.0 36.0 41.0 20-21 39.07699974744844 40.0 39.0 41.0 35.5 41.0 22-23 38.96637691933563 40.0 38.5 41.0 35.0 41.0 24-25 38.86382375048805 40.0 38.0 41.0 35.0 41.0 26-27 38.69752529449985 40.0 38.0 41.0 34.5 41.0 28-29 38.57197419627528 40.0 38.0 41.0 34.0 41.0 30-31 38.32936470168565 40.0 38.0 41.0 34.0 41.0 32-33 38.108177499529745 40.0 38.0 41.0 33.5 41.0 34-35 38.008204178566686 40.0 38.0 41.0 33.0 41.0 36-37 37.953682643862585 40.0 37.5 41.0 33.0 41.0 38-39 37.765433935285344 40.0 37.0 41.0 33.0 41.0 40-41 37.54374275413094 40.0 37.0 41.0 33.0 41.0 42-43 37.28487815698814 40.0 37.0 41.0 32.0 41.0 44-45 37.018256030324174 40.0 36.0 41.0 31.0 41.0 46-47 36.65422806529769 39.0 36.0 41.0 30.5 41.0 48-49 36.248675228439254 39.0 35.0 41.0 30.0 41.0 50-51 35.85931042183604 39.0 35.0 40.0 28.5 41.0 52-53 35.31292899104528 38.0 34.5 40.0 27.0 41.0 54-55 34.91547016931446 38.0 34.0 40.0 26.0 41.0 56-57 34.51887504393423 38.0 34.0 40.0 26.0 41.0 58-59 33.99476386429363 37.5 33.0 40.0 23.5 41.0 60-61 33.49654334106975 37.0 33.0 39.5 22.5 41.0 62-63 32.91835390342039 36.0 32.0 39.0 21.0 40.5 64-65 32.323641074026085 35.5 31.0 39.0 18.5 40.0 66-67 31.747590455799354 35.0 31.0 38.0 14.5 40.0 68-69 31.110575240504772 35.0 30.0 38.0 8.5 40.0 70-71 30.4762770153352 34.5 29.0 37.0 7.0 39.5 72-73 29.79405469652221 34.0 28.5 36.5 2.0 39.0 74-75 29.137814836991836 34.0 28.0 36.0 2.0 39.0 76-77 27.873892463996416 32.0 26.5 34.5 2.0 36.5 78-79 28.509832648996053 33.5 28.0 35.0 2.0 37.0 80-81 28.22462887286315 34.0 27.0 35.0 2.0 37.0 82-83 27.667739948335594 33.0 26.0 35.0 2.0 36.0 84-85 27.01363890822786 33.0 25.5 35.0 2.0 36.0 86-87 26.29028815608136 32.0 24.5 35.0 2.0 35.5 88-89 25.4820317435575 32.0 20.5 34.0 2.0 35.0 90-91 24.826534175771386 31.0 18.5 34.0 2.0 35.0 92-93 23.881760906367823 31.0 8.5 34.0 2.0 35.0 94-95 22.985700485843246 30.5 2.0 34.0 2.0 35.0 96-97 21.9235593132696 30.0 2.0 34.0 2.0 35.0 98-99 20.51825003503497 29.0 2.0 34.0 2.0 35.0 100-101 16.72048725214163 20.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2696479108290859 1101 2 0.23205315312662833 1101 3 0.2148887195995428 1101 4 0.08172445638226122 1101 5 0.015007794259915386 1101 6 0.13557486295513144 1101 7 0.20185963867190537 1101 8 0.14850647657876692 1101 9 0.12173357228863324 1101 10-11 0.18663180325953022 1101 12-13 0.296221102594167 1101 14-15 0.3669836236166404 1101 16-17 0.4127641603751684 1101 18-19 0.3881442045367862 1101 20-21 0.22790765964141713 1101 22-23 0.32786335545004164 1101 24-25 0.3811244787100492 1101 26-27 0.4174548499797268 1101 28-29 0.435366023365205 1101 30-31 0.36469718632923076 1101 32-33 0.3747472041410802 1101 34-35 0.3648312050954132 1101 36-37 0.4597877067857894 1101 38-39 0.53391455189292 1101 40-41 0.5666073412010348 1101 42-43 0.6961510120468972 1101 44-45 0.7651690899760908 1101 46-47 0.8222213197569133 1101 48-49 1.1488525498445412 1101 50-51 1.0131487102917731 1101 52-53 1.0060130024738 1101 54-55 1.1253908923067755 1101 56-57 1.1615732031736243 1101 58-59 1.4834134955547427 1101 60-61 1.3987969155201299 1101 62-63 1.5354178921791615 1101 64-65 1.596647566646304 1101 66-67 1.847686485863715 1101 68-69 2.1446466524498753 1101 70-71 2.2896519543348255 1101 72-73 2.3732430876881345 1101 74-75 2.310071901878487 1101 76-77 2.417800640159335 1101 78-79 2.4134079762643097 1101 80-81 2.392237736002535 1101 82-83 2.4245635878864498 1101 84-85 2.863352493539324 1101 86-87 3.1338252327114056 1101 88-89 3.470321959969681 1101 90-91 3.750491856947935 1101 92-93 3.8671076540188594 1101 94-95 4.03768068024592 1101 96-97 4.579501705368923 1101 98-99 4.25181234759437 1101 100-101 4.014513638784294 1102 1 0.12158689001357459 1102 2 0.09609275069916379 1102 3 0.10091680772344347 1102 4 0.06853222612416232 1102 5 -0.097683129808253 1102 6 -0.0985254789296377 1102 7 0.004871940187200607 1102 8 0.04515113819348926 1102 9 0.05426317397990488 1102 10-11 0.10807175164558203 1102 12-13 0.12063756548369042 1102 14-15 0.18931155073499184 1102 16-17 0.20929519642717054 1102 18-19 0.1763207374737945 1102 20-21 0.11141625572486191 1102 22-23 0.09979208097158221 1102 24-25 0.241381957979506 1102 26-27 0.198265162975666 1102 28-29 0.2574624510005563 1102 30-31 0.18881374397872008 1102 32-33 0.07647650167875497 1102 34-35 0.2549341161564129 1102 36-37 0.38671919734004234 1102 38-39 0.459767391667917 1102 40-41 0.5308167290631189 1102 42-43 0.33381147755167007 1102 44-45 0.22368927903519875 1102 46-47 0.2886812570252246 1102 48-49 0.39978610841283313 1102 50-51 0.477958628694509 1102 52-53 0.7423350913407987 1102 54-55 0.6611725036871903 1102 56-57 0.8053768656898583 1102 58-59 0.9639059738017082 1102 60-61 0.8821390335927859 1102 62-63 1.0412619040112006 1102 64-65 1.2273407257825824 1102 66-67 1.1895816997649185 1102 68-69 1.5253455720709574 1102 70-71 1.808356968548818 1102 72-73 1.993498248362453 1102 74-75 1.9688852276079771 1102 76-77 2.127788672752157 1102 78-79 2.1839070214639555 1102 80-81 2.3319060184851885 1102 82-83 2.3156771039999704 1102 84-85 2.6107955583476574 1102 86-87 2.751132465288098 1102 88-89 2.6492516186897603 1102 90-91 2.7374893626580175 1102 92-93 2.845493955009953 1102 94-95 2.9773792052737846 1102 96-97 2.9402945496946984 1102 98-99 3.5772296947183015 1102 100-101 3.484390263601039 1103 1 0.21845239677966077 1103 2 0.15993888952458946 1103 3 0.13800614907955833 1103 4 0.04249932043094162 1103 5 0.076520937933509 1103 6 0.115506584315348 1103 7 0.15377117835735987 1103 8 0.1778583539578733 1103 9 0.24160957362953894 1103 10-11 0.17572982924566816 1103 12-13 0.21611631040319423 1103 14-15 0.29685202033014235 1103 16-17 0.37345822968713804 1103 18-19 0.30670877973010846 1103 20-21 0.23927172285124954 1103 22-23 0.21030134371779496 1103 24-25 0.3794440899314466 1103 26-27 0.4031261869129139 1103 28-29 0.3266676903590664 1103 30-31 0.42567559641637587 1103 32-33 0.4996923664853412 1103 34-35 0.5076518485758896 1103 36-37 0.4630099284567848 1103 38-39 0.45050638893135186 1103 40-41 0.5168654236867951 1103 42-43 0.5069059963379843 1103 44-45 0.5468128092838285 1103 46-47 0.6861888516123642 1103 48-49 0.8221898614918146 1103 50-51 0.7437935869486836 1103 52-53 0.7280521765814925 1103 54-55 0.7749493915941557 1103 56-57 0.8116788387482501 1103 58-59 0.851392060852838 1103 60-61 0.925657621850398 1103 62-63 1.1335002220158827 1103 64-65 1.246214216279931 1103 66-67 1.2622208920188562 1103 68-69 1.363818676492695 1103 70-71 1.6251474918430695 1103 72-73 1.8763302971017097 1103 74-75 1.8919612655348885 1103 76-77 1.798025632239323 1103 78-79 2.1327167843789354 1103 80-81 1.9619575696220757 1103 82-83 2.1770786784297123 1103 84-85 2.3438203659375034 1103 86-87 2.6899329250081934 1103 88-89 2.7918935168204726 1103 90-91 3.306503516904151 1103 92-93 3.2495814329375285 1103 94-95 3.0072114967050823 1103 96-97 3.0444924246050853 1103 98-99 3.5473314998449617 1103 100-101 3.5264662987383844 1104 1 0.20465546295505987 1104 2 0.16136688467862115 1104 3 0.18220292812790717 1104 4 0.1564908432726142 1104 5 0.028012534807189127 1104 6 0.07048525322644394 1104 7 -0.01359643431771218 1104 8 0.15453662664967283 1104 9 0.2249928429224468 1104 10-11 0.13564561043469325 1104 12-13 0.19589537257230916 1104 14-15 0.2870455761894206 1104 16-17 0.3646306531923287 1104 18-19 0.22953189324650936 1104 20-21 0.2442631252675298 1104 22-23 0.1807800116417937 1104 24-25 0.2582086174789211 1104 26-27 0.2186019034275759 1104 28-29 0.3661402402780283 1104 30-31 0.3442775694187148 1104 32-33 0.40829080569321974 1104 34-35 0.4114556459900953 1104 36-37 0.5901758582033665 1104 38-39 0.535636287135695 1104 40-41 0.44235533460491183 1104 42-43 0.773349163117949 1104 44-45 0.7227786637480946 1104 46-47 0.5658522922383611 1104 48-49 0.7724600947683129 1104 50-51 0.9475359213456684 1104 52-53 0.8558126656007303 1104 54-55 0.7979911134009612 1104 56-57 0.8704895359454952 1104 58-59 1.051356130652188 1104 60-61 1.0782810200445496 1104 62-63 1.165255346511394 1104 64-65 1.173179173805302 1104 66-67 1.4210294176105727 1104 68-69 1.5446436628543516 1104 70-71 1.5774718665358343 1104 72-73 1.9372948321510925 1104 74-75 1.7688035931538266 1104 76-77 1.8127849821527562 1104 78-79 1.6296722592268083 1104 80-81 1.7981711318908857 1104 82-83 1.960760115743028 1104 84-85 2.2085977796606002 1104 86-87 2.2504378035529307 1104 88-89 2.5270570091043645 1104 90-91 2.5299857456031667 1104 92-93 2.861320408584618 1104 94-95 3.1487574034589727 1104 96-97 3.277339208621804 1104 98-99 3.733862544262191 1104 100-101 3.5389987658862694 1105 1 0.15972691091221236 1105 2 0.12807928268569668 1105 3 0.1885459729388117 1105 4 0.1029279070715674 1105 5 -0.08624692871688211 1105 6 -0.01913966678520751 1105 7 0.12463276684458435 1105 8 0.1214021540996768 1105 9 0.17405302652838373 1105 10-11 0.1803317075262285 1105 12-13 0.19681601666214021 1105 14-15 0.3136729811308996 1105 16-17 0.39691855646432117 1105 18-19 0.26994374920025876 1105 20-21 0.2830173238776581 1105 22-23 0.27692258137793857 1105 24-25 0.3182425175986907 1105 26-27 0.3351657783245585 1105 28-29 0.34330652391359706 1105 30-31 0.38096514302667117 1105 32-33 0.46437851027042143 1105 34-35 0.39652241291712187 1105 36-37 0.6406086667467434 1105 38-39 0.6520348942802414 1105 40-41 0.6808805860235339 1105 42-43 0.6250137299608198 1105 44-45 0.7475050226785314 1105 46-47 0.6837961995539601 1105 48-49 0.815436868891183 1105 50-51 0.8621743491171259 1105 52-53 0.8593094324530668 1105 54-55 0.8929587570251627 1105 56-57 0.983436194611258 1105 58-59 0.9687823235056499 1105 60-61 0.9320635662759429 1105 62-63 1.1367570857132776 1105 64-65 1.1283334112580263 1105 66-67 1.309052267511344 1105 68-69 1.2961861618770563 1105 70-71 1.5452082398828502 1105 72-73 1.4013265311907368 1105 74-75 1.335612848645944 1105 76-77 1.2828740088719783 1105 78-79 1.4184507344559414 1105 80-81 1.6548335698610153 1105 82-83 1.8711600948277862 1105 84-85 1.9886400956749526 1105 86-87 2.222367807213093 1105 88-89 2.2534981065569397 1105 90-91 2.681121082151808 1105 92-93 2.9505531678277848 1105 94-95 2.769437721470233 1105 96-97 3.1008077274492543 1105 98-99 3.274591241217781 1105 100-101 3.260237819621011 1106 1 0.1930064083912626 1106 2 0.14639936564456235 1106 3 0.14083628129310455 1106 4 0.09572621928773373 1106 5 -0.054274390962255836 1106 6 0.001004676874281074 1106 7 0.06577309367390427 1106 8 0.09112097598842439 1106 9 0.1646951233852434 1106 10-11 0.17584197606980467 1106 12-13 0.13419935575650044 1106 14-15 0.009174310141467856 1106 16-17 -0.09975474532239303 1106 18-19 -0.003965329789458849 1106 20-21 -0.02895449871433442 1106 22-23 -0.03009167419734382 1106 24-25 -0.02916412387835976 1106 26-27 -0.27297992414394656 1106 28-29 -0.04538317893633348 1106 30-31 0.02526827663097464 1106 32-33 0.08933768640306283 1106 34-35 0.06021122181374494 1106 36-37 0.20289009516492484 1106 38-39 -0.11077933030466625 1106 40-41 0.1035971313112043 1106 42-43 0.1872629760602038 1106 44-45 0.26866757914657313 1106 46-47 0.41057606057402296 1106 48-49 0.2958977316157103 1106 50-51 0.37920237157322845 1106 52-53 0.4185442680252578 1106 54-55 0.4218787422451413 1106 56-57 0.473138448565507 1106 58-59 0.6088871544292829 1106 60-61 0.4740710278061542 1106 62-63 0.16374017211684588 1106 64-65 0.5126558542536017 1106 66-67 1.186431375226757 1106 68-69 1.1535106192474096 1106 70-71 1.138535176483682 1106 72-73 1.5127074506515008 1106 74-75 1.7028417126100877 1106 76-77 1.6968682518127238 1106 78-79 1.7287979459176164 1106 80-81 1.555078811049615 1106 82-83 1.8668232293856342 1106 84-85 2.0184257256736444 1106 86-87 2.162570675015278 1106 88-89 2.3369015655602396 1106 90-91 2.6668914315087555 1106 92-93 2.594790148887892 1106 94-95 2.89999125735352 1106 96-97 3.169947863568545 1106 98-99 3.2019819703260914 1106 100-101 3.2694330232635735 1107 1 0.2830331390468288 1107 2 0.27973505151084055 1107 3 0.2705149546997774 1107 4 0.22865432882166914 1107 5 0.1427920004580585 1107 6 0.1511327428863538 1107 7 0.2004491351843498 1107 8 0.2291234990800035 1107 9 0.27526475983068366 1107 10-11 0.3777484099533339 1107 12-13 0.3705662803195935 1107 14-15 0.5375893792912407 1107 16-17 0.4689606834708471 1107 18-19 0.36729759811422724 1107 20-21 0.2998707933673401 1107 22-23 0.3925788035726825 1107 24-25 0.35581612954824493 1107 26-27 0.40535442371921704 1107 28-29 0.5407174683042939 1107 30-31 0.5230298415358803 1107 32-33 0.6450547825540198 1107 34-35 0.5266067590587511 1107 36-37 0.6568460263415687 1107 38-39 0.6721786269909771 1107 40-41 0.6853688238704336 1107 42-43 0.6572996461674521 1107 44-45 0.6520916320315209 1107 46-47 0.6184376375356706 1107 48-49 0.7698898917996786 1107 50-51 0.7527619597623385 1107 52-53 0.952041396464729 1107 54-55 0.8910749699585736 1107 56-57 1.0006054469393177 1107 58-59 1.0473125453173324 1107 60-61 1.0773939805878783 1107 62-63 1.086966435794615 1107 64-65 1.3004441498140764 1107 66-67 1.5677690254580448 1107 68-69 1.791694287375588 1107 70-71 1.7848680187257564 1107 72-73 1.9274022536121649 1107 74-75 2.090533848386528 1107 76-77 1.9853814215862045 1107 78-79 1.9192798916976166 1107 80-81 2.191599238387223 1107 82-83 2.5004668030008013 1107 84-85 2.260931879017619 1107 86-87 2.437882018046931 1107 88-89 2.8073181697377514 1107 90-91 2.8777795421013295 1107 92-93 3.2400690563715955 1107 94-95 3.6034953551718374 1107 96-97 3.6172399511180586 1107 98-99 3.4445660513238003 1107 100-101 3.666687911385928 1108 1 0.17634879477547827 1108 2 0.18450063910704984 1108 3 0.10288890969554387 1108 4 0.07762313521507025 1108 5 0.0064534743283530815 1108 6 0.09171021130605084 1108 7 0.13310666842193086 1108 8 0.12175046479281804 1108 9 0.13685095656769164 1108 10-11 0.11505828363211634 1108 12-13 0.18483910468522424 1108 14-15 0.2959613823848244 1108 16-17 0.33863622576819097 1108 18-19 0.30981039596345994 1108 20-21 0.2777341970428111 1108 22-23 0.25842603960553845 1108 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2316 54-55 0.1725643794339078 2316 56-57 0.27293361598854915 2316 58-59 0.5069737483211014 2316 60-61 0.7183614730410355 2316 62-63 0.703420044556438 2316 64-65 0.8788167766134691 2316 66-67 1.123708451149735 2316 68-69 1.4734578779251954 2316 70-71 1.4378323677016347 2316 72-73 1.3897576162347214 2316 74-75 1.942308166676078 2316 76-77 1.9984615303605011 2316 78-79 2.0583465783228654 2316 80-81 1.9873540471590232 2316 82-83 2.4031350164578775 2316 84-85 2.6835956017928595 2316 86-87 2.6401592927020197 2316 88-89 2.857709856986709 2316 90-91 2.958257799716776 2316 92-93 3.0437132446808555 2316 94-95 3.5668611212073174 2316 96-97 3.8023690582208225 2316 98-99 4.247058629224657 2316 100-101 4.164846981315822 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 8.0 5 29.0 6 57.0 7 121.0 8 363.0 9 669.0 10 1118.0 11 1962.0 12 2940.0 13 4383.0 14 5949.0 15 8172.0 16 10568.0 17 14002.0 18 14924.0 19 12975.0 20 12711.0 21 13482.0 22 14964.0 23 16377.0 24 17747.0 25 20141.0 26 22505.0 27 26508.0 28 31243.0 29 37288.0 30 44508.0 31 52276.0 32 63431.0 33 79199.0 34 102158.0 35 139011.0 36 191644.0 37 236590.0 38 127967.0 39 6384.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.457977893869895 18.615897558493412 14.582866512637697 23.343258034999 2 16.136824937114554 19.60646753192242 39.79082157323044 24.465885957732585 3 19.868594408330097 23.031276839071243 29.525713797512182 27.57441495508648 4 13.254698705069185 16.510948719120893 36.05998994272148 34.17436263308844 5 13.950527341713078 36.7604854980091 34.31153714275854 14.977450017519283 6 33.34152689524206 35.437704823434984 16.727606283362846 14.493161997960103 7 29.358331690873896 32.06722817546113 19.90458497235797 18.669855161307 8 26.536428610505126 35.30966136256845 19.018238473834607 19.135671553091818 9 25.91673592474713 16.832074197699157 19.939807296416838 37.31138258113687 10-11 25.437674846988976 26.21301562297425 26.720516853882064 21.628792676154713 12-13 26.10506294678956 23.42771667162527 27.347736516032526 23.119483865552642 14-15 22.783223082462957 25.07488490918261 25.595800599678803 26.546091408675633 16-17 22.42624108107055 28.219601448162106 26.551299816169443 22.8028576545979 18-19 22.3927049320996 26.642840388165002 28.81969242667574 22.144762253059657 20-21 22.71843648852914 25.86734972994969 29.295193801470493 22.119019980050673 22-23 22.583272943103786 25.75872485811731 29.15900079212996 22.499001406648947 24-25 22.553438955974077 25.919214492499478 29.1862170472574 22.34112950426905 26-27 22.105839845491904 26.315990643776242 29.064999673986115 22.513169836745735 28-29 22.205539497339966 26.60360871445262 28.965153130927373 22.225698657280045 30-31 22.389744758056356 26.159132961275677 28.87878349586812 22.572338784799843 32-33 22.091216826378673 26.151526438101264 28.99827710376572 22.75897963175435 34-35 22.70155679745594 26.28927734813421 28.823106392339344 22.186059462070514 36-37 22.72035936019317 25.953641845617952 28.743681950641182 22.58231684354769 38-39 22.313865008052993 26.292544257719662 28.77600546209324 22.617585272134104 40-41 22.78311808667752 26.089745919882002 28.78063203558268 22.3465039578578 42-43 22.45536525637927 26.166667603485717 28.592579268943886 22.78538787119113 44-45 22.51369601223086 26.374697413683272 28.71710896105161 22.394497613034257 46-47 22.859213373092093 26.563814982377597 28.065559986240814 22.5114116582895 48-49 22.641884139537357 26.611552472644618 28.032061307827377 22.71450207999065 50-51 22.749874286279557 26.869986907787208 27.9396589130091 22.440479892924134 52-53 22.97173745729293 26.660414079636922 27.76343488104222 22.60441358202792 54-55 22.672122879447475 26.77469928004071 27.94037085360178 22.612806986910037 56-57 22.66103159442468 26.951298017582687 27.761299059264182 22.62637132872845 58-59 22.689584159246873 26.667121309431113 27.7387417836435 22.904552747678512 60-61 22.46329946244738 26.847579514396564 27.935499681125552 22.7536213420305 62-63 22.49342579068497 26.831204880764943 28.03719477420617 22.638174554343923 64-65 22.315590524745183 26.911953932197775 27.930366214746762 22.84208932831028 66-67 22.353885434519828 26.873509140193093 27.754704241142527 23.01790118414456 68-69 22.685686878452003 26.577860713295415 27.917144420213287 22.8193079880393 70-71 22.560685441414407 26.740863366609684 27.53164201228884 23.16680917968706 72-73 22.593959296482787 26.82453512152826 27.47914576121812 23.10235982077083 74-75 22.497659963683535 27.110285593095224 27.380673136083324 23.011381307137917 76-77 22.510437423794254 26.795195674998368 27.60845665518319 23.085910246024184 78-79 22.595420648225655 27.02121807789529 27.544644295744618 22.83871697813443 80-81 22.723195249332836 26.9608904803051 27.35148357178347 22.964430698578592 82-83 22.599973020774 27.090290476333223 27.416963937767918 22.89277256512485 84-85 22.655615902248847 27.102876581227843 27.30590027781796 22.935607238705355 86-87 22.76108916522963 27.101877103563325 27.189823006528307 22.947210724678737 88-89 22.621724979597282 27.275980398401956 26.896066415813774 23.20622820618699 90-91 22.583242777382246 27.19357739723461 27.11181474125979 23.111365084123356 92-93 22.632591441275018 26.963288595985702 27.14715662168451 23.256963341054764 94-95 22.516299704731786 26.821107930274735 27.231485783659824 23.43110658133365 96-97 22.66178100557487 26.604582948947865 27.184926943654027 23.548709101823242 98-99 22.436020896580512 26.829706058464563 26.92319509945061 23.81107794550432 100-101 22.904815041581077 26.30710419288044 27.013836378065935 23.774244387472542 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 516.0 1 365.0 2 315.0 3 440.0 4 355.0 5 299.5 6 555.5 7 1140.5 8 1840.0 9 2189.0 10 1773.5 11 1221.5 12 1149.5 13 1218.5 14 1309.5 15 1409.5 16 1472.0 17 1564.0 18 1743.5 19 1993.0 20 2264.5 21 2582.5 22 3119.0 23 3887.0 24 4858.5 25 6062.5 26 7627.5 27 9526.0 28 11148.0 29 12773.0 30 14671.5 31 17135.5 32 20534.0 33 22216.0 34 22971.0 35 24807.5 36 27412.5 37 29968.0 38 31771.5 39 33480.0 40 35450.0 41 37808.5 42 39430.5 43 41037.0 44 43550.0 45 46314.0 46 47688.0 47 48883.5 48 49780.0 49 49731.5 50 49734.5 51 48975.5 52 48728.5 53 49440.0 54 49636.0 55 46976.5 56 43305.0 57 39878.5 58 36380.0 59 32320.0 60 26537.0 61 21755.0 62 18172.5 63 13788.0 64 9774.0 65 7206.5 66 5402.0 67 4108.0 68 3135.5 69 2418.0 70 1803.5 71 1189.5 72 782.5 73 582.0 74 433.0 75 311.5 76 204.0 77 127.0 78 74.0 79 39.5 80 29.5 81 15.5 82 5.5 83 2.0 84 3.0 85 2.5 86 1.0 87 1.0 88 1.0 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015662693038944648 3 0.002922703485736083 4 0.0017985867604529743 5 0.11720790388951882 6 0.0 7 0.0 8 2.997644600754957E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.997644600754957E-4 24-25 5.995289201509914E-4 26-27 0.006145171431547662 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010154521085057416 36-37 7.119405926793022E-4 38-39 0.007044464811774149 40-41 0.01022946220007629 42-43 0.005882877528981603 44-45 0.0038219968659625697 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 8.243522652076131E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0015737634153963522 84-85 0.004046820211019192 86-87 0.002847762370717209 88-89 0.0 90-91 0.0023231745655850917 92-93 0.0 94-95 7.494111501887393E-5 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1334381.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.01229051707766 #Duplication Level Percentage of deduplicated Percentage of total 1 83.71586359692476 56.099917722451686 2 10.070586567465991 13.497061454728218 3 2.7453485787861047 5.519162895967822 4 1.1528698537919313 3.0902579828274295 5 0.6144828591254181 2.0588951936738513 6 0.36441018313445195 1.465197663575242 7 0.24606994912687377 1.154279764288881 8 0.17337511153379243 0.9294610674026591 9 0.1298301573067187 0.7830194597384155 >10 0.724221223165134 8.636257043742427 >50 0.039436049430684884 1.7914998793051715 >100 0.020595651825847668 2.6993268313140355 >500 0.002014766572353032 0.9003941838549445 >1k 7.835203336928458E-4 0.996104195401005 >5k 1.1193147624183511E-4 0.3791646617283193 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5055 0.37882733642040767 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4705 0.3525979461638018 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2743 0.20556347849677117 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.494111501887391E-5 2 0.0 0.0 0.0 0.0 7.494111501887391E-5 3 0.0 0.0 0.0 0.0 7.494111501887391E-5 4 0.0 0.0 0.0 7.494111501887391E-5 1.4988223003774783E-4 5 0.0 0.0 0.0 1.4988223003774783E-4 1.4988223003774783E-4 6 0.0 0.0 0.0 1.4988223003774783E-4 1.4988223003774783E-4 7 0.0 0.0 0.0 1.4988223003774783E-4 1.4988223003774783E-4 8 0.0 0.0 0.0 1.4988223003774783E-4 1.4988223003774783E-4 9 0.0 0.0 0.0 1.4988223003774783E-4 1.4988223003774783E-4 10-11 0.0 0.0 0.0 1.4988223003774783E-4 1.4988223003774783E-4 12-13 0.0 0.0 0.0 1.4988223003774783E-4 5.245878051321174E-4 14-15 0.0 0.0 0.0 1.4988223003774783E-4 5.245878051321174E-4 16-17 0.0 0.0 0.0 1.4988223003774783E-4 5.245878051321174E-4 18-19 0.0 0.0 0.0 2.2482334505662176E-4 5.245878051321174E-4 20-21 0.0 0.0 0.0 2.2482334505662176E-4 6.744700351698653E-4 22-23 0.0 0.0 0.0 4.496466901132435E-4 7.494111501887392E-4 24-25 0.0 0.0 0.0 6.369994776604283E-4 7.494111501887392E-4 26-27 0.0 0.0 0.0 8.6182282271705E-4 7.494111501887392E-4 28-29 0.0 0.0 0.0 0.0022857040080756547 7.494111501887392E-4 30-31 0.0 0.0 0.0 0.010042109412529105 7.494111501887392E-4 32-33 0.0 0.0 0.0 0.025742273008983192 7.494111501887392E-4 34-35 0.0 0.0 0.0 0.05283348608830611 8.99293380226487E-4 36-37 0.0 0.0 0.0 0.0991470951699702 8.99293380226487E-4 38-39 0.0 0.0 0.0 0.1635215129711829 9.36763937735924E-4 40-41 0.0 0.0 0.0 0.23269216213360353 9.742344952453609E-4 42-43 0.0 0.0 0.0 0.2964670510146652 9.742344952453609E-4 44-45 0.0 0.0 0.0 0.37560486847459607 9.742344952453609E-4 46-47 0.0 0.0 0.0 0.4702554967434338 0.0011241167252831088 48-49 0.0 0.0 0.0 0.5728498832042722 0.0012739989553208566 50-51 0.0 0.0 0.0 0.68147702942413 0.0014988223003774784 52-53 0.0 0.0 0.0 0.7828723580446664 0.001761116202943537 54-55 0.0 0.0 0.0 0.8927360326623355 0.001873527875471848 56-57 0.0 0.0 0.0 1.024145277847931 0.001873527875471848 58-59 0.0 0.0 0.0 1.1672078664189613 0.001873527875471848 60-61 0.0 0.0 0.0 1.3125936295555767 0.001873527875471848 62-63 0.0 0.0 0.0 1.451272162898003 0.001873527875471848 64-65 0.0 0.0 0.0 1.5987562772551467 0.001873527875471848 66-67 0.0 0.0 0.0 1.76291478970399 0.0019484689904907219 68-69 0.0 0.0 0.0 1.9432605829969103 0.0020234101055095956 70-71 0.0 0.0 0.0 2.134397896852548 0.0020234101055095956 72-73 0.0 0.0 0.0 2.3245609762129407 0.0020983512205284697 74-75 0.0 0.0 0.0 2.5185460524392957 0.0023231745655850917 76-77 0.0 0.0 0.0 2.724634118741199 0.0023231745655850917 78-79 0.0 0.0 0.0 2.960960924953218 0.0023606451230945283 80-81 0.0 0.0 0.0 3.212650659744106 0.0023981156806039653 82-83 0.0 0.0 0.0 3.455834577980352 0.0023981156806039653 84-85 0.0 0.0 0.0 3.7073744305412024 0.0023981156806039653 86-87 0.0 0.0 0.0 3.9635981027907325 0.0024355862381134023 88-89 0.0 0.0 0.0 4.231699941770754 0.0024730567956228393 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2550 0.0 47.38178 1 GTATCAA 4470 0.0 40.225563 1 ATCAACG 5470 0.0 32.427166 3 TCAACGC 5510 0.0 32.27806 4 TATCAAC 5595 0.0 31.797234 2 CAACGCA 5625 0.0 31.702698 5 AACGCAG 5755 0.0 30.948246 6 TGGTATC 1285 0.0 27.763613 2 ACGCAGA 6565 0.0 26.98511 7 CGCAGAG 6670 0.0 26.560308 8 GTGGTAT 1435 0.0 25.19298 1 GCAGAGT 7350 0.0 23.909168 9 GAGTACT 4875 0.0 21.336323 12-13 GTACCTG 930 0.0 20.970982 1 AGAGTAC 6560 0.0 20.96017 10-11 GTACTTT 4990 0.0 20.749426 14-15 ACCGTGC 275 1.2605597E-9 20.725197 8 GGACCGA 390 0.0 20.703054 6 GTACATG 3860 0.0 20.580076 1 CGTACAC 185 6.4892392E-6 20.563911 3 >>END_MODULE