FastQCFastQC Report
Thu 26 May 2016
SRR938062_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938062_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315872
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49610.3770123537851706No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44620.33909073222927455No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26030.19781559300600668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.051.6254431
GTATCAA43450.038.8907551
ATCAACG53800.031.0077363
TCAACGC54150.030.8073144
TATCAAC55000.030.5040282
CAACGCA55650.030.0623345
AACGCAG57750.029.2104996
TGGTATC12250.025.6066672
ACGCAGA65700.025.5215347
CGCAGAG66300.025.0756348
GTGGTAT14150.024.6263071
GCAGAGT72150.022.3841199
GTCGTAA1100.00279648921.6975251
GTACATG39500.021.6316071
GAGTACT46850.021.14066512-13
TACATGG38650.021.0277022
CAGAGTA67800.020.94822310-11
GGACCGA3302.7284841E-1120.1593576
GTACTTT48500.020.13105814-15
ACATGGG38750.020.1148743
Produced by FastQC (version 0.11.5)