##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938062_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1315872 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.51260912915542 33.0 31.0 34.0 30.0 34.0 2 31.72002064030544 33.0 31.0 34.0 30.0 34.0 3 31.797363269375744 34.0 31.0 34.0 30.0 34.0 4 35.33047059288442 37.0 35.0 37.0 33.0 37.0 5 35.327676248145714 37.0 35.0 37.0 33.0 37.0 6 35.3357765800929 37.0 35.0 37.0 33.0 37.0 7 35.26698189489555 37.0 35.0 37.0 33.0 37.0 8 35.22274278957224 37.0 35.0 37.0 32.0 37.0 9 36.80360019819557 39.0 37.0 39.0 33.0 39.0 10-11 36.69272581223706 39.0 37.0 39.0 32.0 39.0 12-13 36.57179345711437 39.0 37.0 39.0 32.0 39.0 14-15 37.68247557513193 40.0 38.0 41.0 32.0 41.0 16-17 37.4977311623015 40.0 37.0 41.0 32.0 41.0 18-19 37.63517424187155 40.0 38.0 41.0 32.0 41.0 20-21 37.59955451594076 40.0 38.0 41.0 32.0 41.0 22-23 37.483148056953866 40.0 37.5 41.0 32.0 41.0 24-25 37.357076524160405 40.0 37.0 41.0 31.5 41.0 26-27 37.15625151990467 40.0 37.0 41.0 31.0 41.0 28-29 36.922439264609324 40.0 36.5 41.0 30.5 41.0 30-31 36.68167648525085 39.5 36.0 41.0 30.0 41.0 32-33 36.40955579266068 39.0 35.5 41.0 30.0 41.0 34-35 36.13938893752584 39.0 35.0 41.0 29.5 41.0 36-37 35.849176819629875 39.0 35.0 40.0 27.5 41.0 38-39 35.520095039639116 38.5 35.0 40.0 26.5 41.0 40-41 35.270348103766935 38.0 35.0 40.0 25.5 41.0 42-43 35.06163745409888 38.0 34.5 40.0 24.5 41.0 44-45 34.75828500036478 38.0 34.0 40.0 24.0 41.0 46-47 34.34906814644586 38.0 33.5 40.0 23.0 41.0 48-49 33.93929158763163 38.0 33.0 40.0 20.5 41.0 50-51 33.25369070851876 37.5 32.5 39.5 19.0 40.5 52-53 33.40254181257751 38.0 33.0 39.5 19.5 40.5 54-55 33.46948069417086 38.0 33.0 40.0 18.0 41.0 56-57 33.12346071654386 37.5 32.5 40.0 14.5 41.0 58-59 32.76548326888937 37.0 32.0 40.0 11.0 41.0 60-61 32.29977041839936 36.5 31.5 39.5 8.0 41.0 62-63 31.699339297439266 36.0 31.0 39.0 4.5 40.5 64-65 31.128912234624643 35.0 30.0 39.0 2.0 40.0 66-67 30.537189787456533 35.0 29.0 38.0 2.0 40.0 68-69 29.87098023211984 34.5 28.5 38.0 2.0 40.0 70-71 29.26176292222952 34.0 28.0 37.0 2.0 39.0 72-73 28.560795426910822 34.0 26.5 36.5 2.0 39.0 74-75 27.813095042678924 33.5 26.0 36.0 2.0 39.0 76-77 27.087042660684325 33.0 24.5 35.0 2.0 37.5 78-79 26.30819335011308 32.5 22.5 35.0 2.0 37.0 80-81 25.595114494418908 32.0 19.5 35.0 2.0 36.5 82-83 24.80876255441259 31.0 16.0 35.0 2.0 36.0 84-85 24.10198940322463 31.0 8.5 34.0 2.0 35.5 86-87 23.36213400695508 30.5 4.0 34.0 2.0 35.0 88-89 22.494464887162277 29.5 2.0 34.0 2.0 35.0 90-91 21.605334713406776 29.0 2.0 34.0 2.0 35.0 92-93 20.66859162593322 28.5 2.0 34.0 2.0 35.0 94-95 19.66474398725712 26.5 2.0 33.5 2.0 35.0 96-97 18.432183753434984 25.0 2.0 33.0 2.0 35.0 98-99 16.93173348167603 11.5 2.0 32.5 2.0 34.0 100-101 13.601788775807982 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.559227254863 1101 2 0.5353934229088608 1101 3 0.5502666625958454 1101 4 0.4988445912984645 1101 5 0.446190579964707 1101 6 0.4949279422827857 1101 7 0.542521025567801 1101 8 0.3550589581588639 1101 9 0.5590583940603366 1101 10-11 0.6139479548481219 1101 12-13 0.6240703451230516 1101 14-15 0.8984541917164606 1101 16-17 0.9629842858767219 1101 18-19 0.8241116945192815 1101 20-21 0.8400880199961662 1101 22-23 0.8228300158662591 1101 24-25 0.7579161910572623 1101 26-27 1.0343565251297306 1101 28-29 0.9804186324426425 1101 30-31 0.9865448934287286 1101 32-33 0.994068396750329 1101 34-35 1.1228489435838398 1101 36-37 1.3600900083937475 1101 38-39 1.3555623882046532 1101 40-41 1.4204040187146845 1101 42-43 1.5109374514224143 1101 44-45 1.555402996891722 1101 46-47 1.6729412583691783 1101 48-49 1.7091960115118496 1101 50-51 1.5620649896839254 1101 52-53 1.6268008239076721 1101 54-55 1.7863771156795494 1101 56-57 1.9207619020449798 1101 58-59 1.935633857463138 1101 60-61 2.0386621262755327 1101 62-63 2.158362700799419 1101 64-65 1.8997073783271468 1101 66-67 1.8906293317188023 1101 68-69 2.323034090286672 1101 70-71 2.659772795278972 1101 72-73 2.7071768842339523 1101 74-75 2.8878486567534445 1101 76-77 2.9814174935647486 1101 78-79 3.100036907532374 1101 80-81 3.329458720088855 1101 82-83 3.4844386734223534 1101 84-85 3.767172398115246 1101 86-87 4.061422244166401 1101 88-89 4.347000601459367 1101 90-91 4.67767265824801 1101 92-93 4.836900644190532 1101 94-95 4.967683087002374 1101 96-97 5.235754952613803 1101 98-99 5.098442694243012 1101 100-101 4.414531034745181 1102 1 0.29207369769674685 1102 2 0.2102461332747403 1102 3 0.2807995868570359 1102 4 0.08376058165529798 1102 5 0.1397860770162822 1102 6 0.3026114529420596 1102 7 0.3037034701682373 1102 8 0.4354425553121857 1102 9 0.5567494762349412 1102 10-11 0.3956681347767699 1102 12-13 0.5424031395117268 1102 14-15 0.6038013115172944 1102 16-17 0.5131403169304463 1102 18-19 0.40782754711660374 1102 20-21 0.505552577154539 1102 22-23 0.5336191623093569 1102 24-25 0.46190444732150837 1102 26-27 0.6007962435114251 1102 28-29 0.5917471836279304 1102 30-31 0.5341984466514802 1102 32-33 0.6065425339787822 1102 34-35 0.5955044028304215 1102 36-37 0.5582560191253236 1102 38-39 0.5512200058589016 1102 40-41 0.5780752388065409 1102 42-43 0.5995583577433123 1102 44-45 0.6845627295751697 1102 46-47 0.8219913355302779 1102 48-49 1.0245377944459761 1102 50-51 1.0365453024295803 1102 52-53 0.8667041556373078 1102 54-55 1.0763467638932767 1102 56-57 1.0297700335369626 1102 58-59 1.1252000858757683 1102 60-61 1.2899424484855686 1102 62-63 1.1757301927086736 1102 64-65 1.4644970716019081 1102 66-67 1.3515150938522922 1102 68-69 1.5891051573708204 1102 70-71 1.7675186789247626 1102 72-73 1.9527365059736645 1102 74-75 1.8481566964933798 1102 76-77 2.022021716261939 1102 78-79 2.5771659361437607 1102 80-81 2.7565040151019105 1102 82-83 3.1209888491708924 1102 84-85 2.9928939267200647 1102 86-87 2.9071693792154214 1102 88-89 3.111258316912359 1102 90-91 3.725059696760205 1102 92-93 4.033267434157224 1102 94-95 4.380104907163112 1102 96-97 4.575965088454147 1102 98-99 4.378487873580141 1102 100-101 3.9409629844366663 1103 1 0.6356410907556658 1103 2 0.657526197842941 1103 3 0.605141798317888 1103 4 0.5077153839151833 1103 5 0.6306981028435601 1103 6 0.5265178290518264 1103 7 0.6748414846960813 1103 8 0.683287591631796 1103 9 0.6785573858394613 1103 10-11 0.7680490871577277 1103 12-13 0.8753732767191522 1103 14-15 1.1254962267715243 1103 16-17 1.135061220885241 1103 18-19 0.9225652388034433 1103 20-21 1.0546001962021592 1103 22-23 1.140850800727435 1103 24-25 1.0568358436088374 1103 26-27 1.0884475752305534 1103 28-29 1.2174211142171103 1103 30-31 1.2157561544803812 1103 32-33 1.3756466502015385 1103 34-35 1.3315173164704532 1103 36-37 1.312953355687064 1103 38-39 1.5165388113472034 1103 40-41 1.5068000168533544 1103 42-43 1.6183719170653532 1103 44-45 1.833658774772907 1103 46-47 1.6203609884762002 1103 48-49 1.5915676572385422 1103 50-51 1.4721223807185169 1103 52-53 1.6674709030142338 1103 54-55 1.7831748510603447 1103 56-57 1.892288193343255 1103 58-59 2.0122299486683417 1103 60-61 1.9589335703580488 1103 62-63 2.2678205881889006 1103 64-65 2.3289845615131206 1103 66-67 2.4780606951436575 1103 68-69 2.7090648022457238 1103 70-71 2.868301568351722 1103 72-73 3.0201457069421913 1103 74-75 3.1191252194958032 1103 76-77 3.2231113046396658 1103 78-79 3.7812498748039722 1103 80-81 3.6072142653036856 1103 82-83 4.051230979921499 1103 84-85 3.8001094230881023 1103 86-87 3.807936126592338 1103 88-89 4.277569858478138 1103 90-91 4.353574628156494 1103 92-93 4.518101978144312 1103 94-95 4.947610960431877 1103 96-97 5.180549430665607 1103 98-99 5.29985187680855 1103 100-101 5.110777350861202 1104 1 0.2257768437274592 1104 2 0.3686790430282798 1104 3 0.3683421922125234 1104 4 0.11376166653458597 1104 5 0.1530502165103016 1104 6 0.04640528772805652 1104 7 0.2508758097998296 1104 8 0.2646873243461556 1104 9 0.27381236227611794 1104 10-11 0.31557036450174536 1104 12-13 0.19900695471029195 1104 14-15 0.3206752378146476 1104 16-17 0.7086576195628851 1104 18-19 0.4105704438396671 1104 20-21 0.26870584436984046 1104 22-23 0.2987019648267548 1104 24-25 0.32186292386486315 1104 26-27 0.3641316154640748 1104 28-29 0.3542903552665635 1104 30-31 0.45890871831240787 1104 32-33 0.3798134927140282 1104 34-35 0.42307953072099735 1104 36-37 0.6981112652319794 1104 38-39 0.6057566491470183 1104 40-41 0.7778086684676211 1104 42-43 0.751703140248857 1104 44-45 0.8864519097521608 1104 46-47 0.8481664326627438 1104 48-49 0.9739514231525987 1104 50-51 1.0340348798392895 1104 52-53 1.0518557943415132 1104 54-55 1.2226817793855886 1104 56-57 1.438835988236697 1104 58-59 1.384401292833779 1104 60-61 1.3957774896096495 1104 62-63 1.4928469650616556 1104 64-65 1.7953417395394276 1104 66-67 1.7467270804570099 1104 68-69 1.8165495036595196 1104 70-71 1.9276417948411328 1104 72-73 2.0027855043678073 1104 74-75 2.0860176721932753 1104 76-77 2.215799635511157 1104 78-79 2.657343284102698 1104 80-81 2.629294169643561 1104 82-83 2.868881679787858 1104 84-85 3.2120690488881998 1104 86-87 3.035100788616674 1104 88-89 3.091248552998781 1104 90-91 3.273199724871695 1104 92-93 3.629508744691403 1104 94-95 3.7436881919153073 1104 96-97 4.0354773338800705 1104 98-99 3.96143941285694 1104 100-101 4.423856762832784 1105 1 0.32747752510865524 1105 2 0.3859967118763663 1105 3 0.18817343923032226 1105 4 0.1797683640476322 1105 5 0.20863566197317596 1105 6 0.3186822356533199 1105 7 0.3475756379600341 1105 8 0.2231425269834233 1105 9 0.4280959120667305 1105 10-11 0.39351318891321085 1105 12-13 0.3495822539082596 1105 14-15 0.5755687590957166 1105 16-17 0.593011394332116 1105 18-19 0.5350513104948149 1105 20-21 0.5182194540598246 1105 22-23 0.4477865728287682 1105 24-25 0.44740243669198065 1105 26-27 0.4409231349908822 1105 28-29 0.5699313633498804 1105 30-31 0.48116664538756027 1105 32-33 0.6167230719402212 1105 34-35 0.6689881458618103 1105 36-37 0.6802202536162198 1105 38-39 0.4776436152996908 1105 40-41 0.6569387743431037 1105 42-43 0.8322613315507894 1105 44-45 0.8461774334504994 1105 46-47 1.0468237236806601 1105 48-49 1.2983531087259834 1105 50-51 1.2750173665274147 1105 52-53 1.0744876169845838 1105 54-55 1.4239905769091834 1105 56-57 1.27269185149526 1105 58-59 1.299257174569746 1105 60-61 1.3273809581431877 1105 62-63 1.4593795075623852 1105 64-65 1.5232240233022516 1105 66-67 1.7518512974773053 1105 68-69 1.7558208203262389 1105 70-71 2.105998404260479 1105 72-73 2.2783881120720118 1105 74-75 2.5759306436522174 1105 76-77 2.525797103020828 1105 78-79 2.6772217028287777 1105 80-81 2.855031988419423 1105 82-83 2.9443572282133132 1105 84-85 3.0827878631175345 1105 86-87 3.167039458874477 1105 88-89 3.5330602400612356 1105 90-91 3.6282608470149 1105 92-93 3.7218158433813535 1105 94-95 3.643565969367014 1105 96-97 4.038127481753591 1105 98-99 4.054115516039076 1105 100-101 3.869286759039486 1106 1 0.26077566632502425 1106 2 0.1727228852135383 1106 3 0.19919976933238104 1106 4 0.21525161579923946 1106 5 0.30175400346467995 1106 6 0.021569588886194424 1106 7 0.20055577041036798 1106 8 0.14599980695270887 1106 9 0.270400035942977 1106 10-11 0.2551401844662138 1106 12-13 0.3134167549800182 1106 14-15 0.39602417708209003 1106 16-17 0.1405063554608148 1106 18-19 0.4216439137516872 1106 20-21 0.3923020595561937 1106 22-23 0.3126520429634567 1106 24-25 0.2348068581015923 1106 26-27 0.33461734748728134 1106 28-29 0.46008156970365377 1106 30-31 0.5430335871925962 1106 32-33 0.5330690104499851 1106 34-35 0.5092295317913624 1106 36-37 0.5056241631404816 1106 38-39 0.2839094117612788 1106 40-41 0.7155888367291325 1106 42-43 0.8354630143738362 1106 44-45 0.9249941784996096 1106 46-47 1.2703920443768268 1106 48-49 1.23147448953668 1106 50-51 1.1120602689172756 1106 52-53 1.2814460582937386 1106 54-55 0.5942100477477155 1106 56-57 1.1199900566972971 1106 58-59 1.415780673306024 1106 60-61 1.4389335703580528 1106 62-63 1.3521891182095978 1106 64-65 1.4967899445358945 1106 66-67 1.984416802804109 1106 68-69 1.5909281562829882 1106 70-71 1.6940986698009972 1106 72-73 2.010849640689603 1106 74-75 2.3334937495165065 1106 76-77 2.4828939133353174 1106 78-79 2.7186308271849207 1106 80-81 2.836282588285055 1106 82-83 2.804150234579886 1106 84-85 2.956413770914189 1106 86-87 2.7547270168614872 1106 88-89 2.915882487877724 1106 90-91 3.1476533031047325 1106 92-93 3.427636139634995 1106 94-95 3.2830043351730787 1106 96-97 3.5072368012660213 1106 98-99 3.784862228775424 1106 100-101 3.8660050941324258 1107 1 0.2598067456861912 1107 2 0.22887922630645008 1107 3 -0.021071610558408338 1107 4 0.07435372196995615 1107 5 0.21995616300814191 1107 6 0.09745537120749503 1107 7 0.07029137003264907 1107 8 0.024227831750508244 1107 9 0.16842113870604436 1107 10-11 0.03803627183329894 1107 12-13 0.12225712941036448 1107 14-15 0.15342554013994203 1107 16-17 0.25540453668182295 1107 18-19 0.02577311685551109 1107 20-21 0.33303470314941563 1107 22-23 0.3311590430045115 1107 24-25 0.3258679493689911 1107 26-27 0.3697068494784901 1107 28-29 0.5120460973548404 1107 30-31 0.5193073483290291 1107 32-33 0.4751657382900376 1107 34-35 0.3227974999319372 1107 36-37 0.5613258094844511 1107 38-39 0.4973819247965494 1107 40-41 0.48741110548144206 1107 42-43 0.7540767599090046 1107 44-45 0.6155574662608316 1107 46-47 0.5975382446478008 1107 48-49 0.736131261717162 1107 50-51 0.6275506823507868 1107 52-53 0.6417829224395675 1107 54-55 0.8595885836922079 1107 56-57 0.7133667040676599 1107 58-59 0.7560401469686227 1107 60-61 0.8536172341918586 1107 62-63 1.0722418751172675 1107 64-65 1.304709639079558 1107 66-67 1.4961464903682646 1107 68-69 1.2914423821728747 1107 70-71 1.3791891981911952 1107 72-73 1.5741096891356356 1107 74-75 1.440397309068178 1107 76-77 1.8982529476991559 1107 78-79 2.1386969272793515 1107 80-81 2.367127930486472 1107 82-83 2.3400054302115336 1107 84-85 2.306162303163646 1107 86-87 2.557787738625251 1107 88-89 2.9423789775932043 1107 90-91 3.2010506837345325 1107 92-93 3.4410994150794245 1107 94-95 3.4862754687281132 1107 96-97 3.8351784607478976 1107 98-99 3.8553368356646125 1107 100-101 3.348528598669109 1108 1 0.07677717309557863 1108 2 -0.08142370770067231 1108 3 1.1671851852668169E-4 1108 4 -0.11100782629913652 1108 5 -0.03923610339707295 1108 6 -0.016298925556995414 1108 7 -0.07390843387599944 1108 8 -0.06713387928214587 1108 9 -0.14485998110731657 1108 10-11 -0.155452313402499 1108 12-13 -0.05615887102366912 1108 14-15 0.05962625087419582 1108 16-17 0.11794246640670991 1108 18-19 -0.06353273526990932 1108 20-21 -0.11896699811743616 1108 22-23 -0.1637541110046996 1108 24-25 -0.12838531462190161 1108 26-27 -0.20052708440074696 1108 28-29 -0.0767545095207538 1108 30-31 -0.1917286999264931 1108 32-33 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-2.861455485997645 2316 82-83 -2.8151773485920906 2316 84-85 -2.9420093353464445 2316 86-87 -2.7379767324784154 2316 88-89 -2.9946452947896454 2316 90-91 -3.1906879513692417 2316 92-93 -3.570085798990835 2316 94-95 -3.635793705078054 2316 96-97 -3.5892866981178884 2316 98-99 -3.518021653170244 2316 100-101 -3.1757466514522736 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5726.0 3 2060.0 4 2134.0 5 2320.0 6 2732.0 7 3290.0 8 3820.0 9 4682.0 10 5808.0 11 6810.0 12 8132.0 13 9467.0 14 10633.0 15 12548.0 16 14095.0 17 16564.0 18 16642.0 19 15452.0 20 16067.0 21 16895.0 22 18705.0 23 20685.0 24 22880.0 25 25466.0 26 28290.0 27 31905.0 28 36304.0 29 41626.0 30 47993.0 31 56316.0 32 67696.0 33 82825.0 34 104775.0 35 134633.0 36 166626.0 37 169901.0 38 79260.0 39 4109.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.31332249713126 18.149773210862364 14.14857303404242 23.38833125796396 2 15.81371444385143 19.254641857381582 40.20043883057582 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22.47680237631228 46-47 22.89654087189732 26.60390611459274 27.886369648705106 22.613183364804833 48-49 22.655349665978452 26.614614848983344 27.92056003721824 22.809475447819967 50-51 22.74103379736781 26.890056892679198 27.853622015224587 22.5152872947284 52-53 22.945010643064563 26.67599288714171 27.793847202760922 22.585149267032797 54-55 22.665548322464694 26.736470701801522 27.95443722824445 22.643543747489332 56-57 22.63094427792289 26.949888856838715 27.705905580188585 22.71326128504981 58-59 22.632890475924448 26.63821707803799 27.74256732644934 22.986325119588223 60-61 22.500253682963564 26.789453021525617 27.9642616761622 22.74603161934862 62-63 22.488026639728982 26.811927982736773 28.102907430590236 22.59713794694401 64-65 22.361738669086826 26.8403268280132 27.948249317524766 22.84968518537521 66-67 22.36166276770229 26.824000899877937 27.765794605900584 23.04854172651919 68-69 22.60606187283026 26.516427785578966 27.998206254168462 22.87930408742231 70-71 22.517934537228893 26.63755740648121 27.68212353433899 23.1623845219509 72-73 22.53798718647412 26.724881635643765 27.633830201399928 23.103300976482185 74-75 22.47062910008794 26.9741417510278 27.544572245535175 23.010656903349084 76-77 22.63523270696464 26.656720365545567 27.57411843749335 23.133928489996443 78-79 22.712112655502157 26.744774663913162 27.543805110298504 22.999307570286177 80-81 22.788377781392473 26.75560078989494 27.330007646596506 23.126013782116086 82-83 22.743367439428205 26.88979853676834 27.350407536353714 23.016426487449735 84-85 22.720713729511075 26.918883421925006 27.27394682827632 23.0864560202876 86-87 22.853979156707723 26.776821128133882 27.222095136185143 23.14710457897325 88-89 22.867233683813634 26.837912078514275 26.98295413729052 23.31190010038157 90-91 22.82554985571324 26.755957720482034 27.04209858936505 23.376393834439686 92-93 22.90526848652437 26.493310449562717 27.182141841880036 23.419279222032877 94-95 22.798550592978977 26.306859064438402 27.299154765972766 23.59543557660986 96-97 23.073215804010864 26.002326869258606 27.185373790907878 23.73908353582265 98-99 22.828636334094366 26.16989164513239 26.930720765678846 24.070751255094397 100-101 23.754760549316398 25.120707133745938 27.016745260760892 24.107787056176765 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 525.0 1 362.0 2 341.5 3 483.0 4 365.5 5 301.0 6 558.5 7 1170.5 8 1834.5 9 2084.5 10 1720.0 11 1225.5 12 1117.5 13 1156.5 14 1253.0 15 1356.5 16 1438.0 17 1564.5 18 1740.5 19 1941.5 20 2217.5 21 2593.5 22 3128.5 23 3803.5 24 4643.0 25 5851.0 26 7323.0 27 9242.0 28 11141.5 29 12552.0 30 14126.5 31 16604.5 32 19867.5 33 21705.0 34 22608.0 35 24222.0 36 26725.0 37 29333.5 38 31180.0 39 32902.5 40 34621.0 41 36859.5 42 38966.5 43 40390.5 44 42329.0 45 44886.0 46 46409.5 47 47645.0 48 48679.0 49 48975.5 50 48766.0 51 47880.5 52 47629.5 53 48262.0 54 48307.5 55 45953.0 56 42370.5 57 39125.0 58 36284.5 59 32336.0 60 26815.5 61 22302.0 62 18560.5 63 14412.5 64 10766.5 65 8171.0 66 6149.0 67 4674.5 68 3587.0 69 2751.0 70 2135.5 71 1461.0 72 1003.5 73 740.5 74 509.0 75 350.0 76 258.0 77 181.5 78 110.0 79 61.0 80 45.0 81 31.5 82 14.0 83 10.0 84 10.5 85 9.0 86 8.5 87 6.0 88 2.5 89 1.0 90 1.5 91 2.0 92 1.5 93 2.0 94 1.5 95 1.0 96 1.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4609110916563314 2 0.04240534033705406 3 0.055704506213370295 4 0.059504267892317796 5 0.08313878553537121 6 0.08374674740400281 7 0.049700882760633246 8 0.044457211643685705 9 0.049700882760633246 10-11 0.053158665888475475 12-13 0.04863694949052795 14-15 0.041531395150896135 16-17 0.04187337370200141 18-19 0.04920691374237008 20-21 0.057680382286423 22-23 0.0494728970598964 24-25 0.06584986989616011 26-27 0.05794636560394932 28-29 0.04544514968021206 30-31 0.04654708056710683 32-33 0.04650908295031735 34-35 0.051182789815422784 36-37 0.04536915444663311 38-39 0.028346222124948327 40-41 0.0268643150701588 42-43 0.026408343668685098 44-45 0.016718951387368983 46-47 0.03009411249726417 48-49 0.029486150628632572 50-51 0.018010870358211133 52-53 0.01721292040563216 54-55 0.017972872741421658 56-57 0.01721292040563216 58-59 0.016072991901947907 60-61 0.019264791712263807 62-63 0.018466841759684834 64-65 0.019492777413000657 66-67 0.010487342233895091 68-69 0.014401096763211012 70-71 0.019302789329053282 72-73 0.017554898956737435 74-75 0.015123051482211035 76-77 0.01063933270105299 78-79 0.016338975219474233 80-81 0.019302789329053282 82-83 0.01690893947131636 84-85 0.01455308723036891 86-87 0.004027747379684346 88-89 0.029752133946158898 90-91 0.033133921840422166 92-93 0.018086865591790083 94-95 0.033247914690790595 96-97 0.028156234041000948 98-99 0.025344410398579802 100-101 0.14803871501179447 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1315872.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.5817908691289 #Duplication Level Percentage of deduplicated Percentage of total 1 85.4070863145723 59.42778018682211 2 9.117686206286594 12.688498696323503 3 2.385008319530587 4.978594503321296 4 1.012898776214188 2.8191724327252885 5 0.5382211068314734 1.872519424844933 6 0.3439389016599739 1.4359130836237317 7 0.22716259186685386 1.1064465972398094 8 0.1639489386521589 0.9126288610008132 9 0.12008852699658537 0.7520377294132329 >10 0.6308573305483951 7.872813015969263 >50 0.032521072017189116 1.5343700455190143 >100 0.018063942788829914 2.4931851044767233 >500 0.0016421556749348942 0.7598564827733556 >1k 8.75816359965277E-4 1.3461838359469414 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4961 0.3770123537851706 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4462 0.33909073222927455 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2603 0.19781559300600668 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.599523357894993E-5 0.0 10-11 0.0 0.0 0.0 7.599523357894993E-5 0.0 12-13 0.0 0.0 0.0 1.1399285036842489E-4 4.939690182631745E-4 14-15 3.7997616789474965E-5 0.0 0.0 1.5199046715789986E-4 6.079618686315994E-4 16-17 7.599523357894993E-5 0.0 0.0 2.2798570073684978E-4 6.839571022105494E-4 18-19 7.599523357894993E-5 0.0 0.0 2.2798570073684978E-4 7.599523357894993E-4 20-21 7.599523357894993E-5 0.0 0.0 5.319666350526495E-4 9.499404197368741E-4 22-23 7.599523357894993E-5 0.0 0.0 7.219547190000244E-4 9.87938036526349E-4 24-25 7.599523357894993E-5 0.0 0.0 0.001177926120473724 0.001063933270105299 26-27 7.599523357894993E-5 0.0 0.0 0.0017858879891053231 0.001063933270105299 28-29 7.599523357894993E-5 0.0 0.0 0.003989749762894871 0.001063933270105299 30-31 7.599523357894993E-5 0.0 0.0 0.012235232606210938 0.0011019308868947739 32-33 7.599523357894993E-5 0.0 0.0 0.029486150628632572 0.0012159237372631989 34-35 7.599523357894993E-5 0.0 0.0 0.05505854672794922 0.0012159237372631989 36-37 7.599523357894993E-5 0.0 0.0 0.0987938036526349 0.0012159237372631989 38-39 7.599523357894993E-5 0.0 0.0 0.16730350672405825 0.0012159237372631989 40-41 7.599523357894993E-5 0.0 0.0 0.23379933610563947 0.0012159237372631989 42-43 7.599523357894993E-5 0.0 0.0 0.30432291286690494 0.0012159237372631989 44-45 7.599523357894993E-5 0.0 0.0 0.3894375744753289 0.0012539213540526738 46-47 7.599523357894993E-5 0.0 0.0 0.4867874686899638 0.0012919189708421488 48-49 7.599523357894993E-5 0.0 0.0 0.5874051579484936 0.0012919189708421488 50-51 7.599523357894993E-5 0.0 0.0 0.6939124778093918 0.0013679142044210988 52-53 7.599523357894993E-5 0.0 0.0 0.7949861384693953 0.0013679142044210988 54-55 7.599523357894993E-5 0.0 0.0 0.9083710269691885 0.0013679142044210988 56-57 7.599523357894993E-5 7.599523357894993E-5 0.0 1.0394248072760877 0.0013679142044210988 58-59 7.599523357894993E-5 7.599523357894993E-5 0.0 1.1810419250504607 0.0013679142044210988 60-61 7.599523357894993E-5 7.599523357894993E-5 0.0 1.322165073806571 0.0013679142044210988 62-63 7.599523357894993E-5 7.599523357894993E-5 0.0 1.459944432285207 0.0014439094380000487 64-65 7.599523357894993E-5 7.599523357894993E-5 0.0 1.6017515381435277 0.0014439094380000487 66-67 7.599523357894993E-5 7.599523357894993E-5 0.0 1.7661672259915857 0.0014439094380000487 68-69 1.5199046715789986E-4 7.599523357894993E-5 0.0 1.951481603073855 0.0014439094380000487 70-71 1.5199046715789986E-4 7.599523357894993E-5 0.0 2.1463713795870722 0.0014439094380000487 72-73 1.5199046715789986E-4 7.599523357894993E-5 0.0 2.3363214659176577 0.0015579022883684735 74-75 1.5199046715789986E-4 7.599523357894993E-5 0.0 2.5263855450986115 0.0016338975219474233 76-77 1.5199046715789986E-4 7.599523357894993E-5 0.0 2.7311926995938816 0.0016718951387368983 78-79 1.5199046715789986E-4 1.1399285036842489E-4 0.0 2.9646120595316265 0.0016718951387368983 80-81 1.5199046715789986E-4 1.5199046715789986E-4 0.0 3.20897473310474 0.0016718951387368983 82-83 1.5199046715789986E-4 1.5199046715789986E-4 0.0 3.4484737117288002 0.0016718951387368983 84-85 2.2798570073684978E-4 1.5199046715789986E-4 0.0 3.6892266117069137 0.0016718951387368983 86-87 2.2798570073684978E-4 1.5199046715789986E-4 0.0 3.939022944480922 0.0017478903723158483 88-89 2.2798570073684978E-4 1.5199046715789986E-4 0.0 4.208046071350405 0.0018618832226842733 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2515 0.0 51.625443 1 GTATCAA 4345 0.0 38.890755 1 ATCAACG 5380 0.0 31.007736 3 TCAACGC 5415 0.0 30.807314 4 TATCAAC 5500 0.0 30.504028 2 CAACGCA 5565 0.0 30.062334 5 AACGCAG 5775 0.0 29.210499 6 TGGTATC 1225 0.0 25.606667 2 ACGCAGA 6570 0.0 25.521534 7 CGCAGAG 6630 0.0 25.075634 8 GTGGTAT 1415 0.0 24.626307 1 GCAGAGT 7215 0.0 22.384119 9 GTCGTAA 110 0.002796489 21.697525 1 GTACATG 3950 0.0 21.631607 1 GAGTACT 4685 0.0 21.140665 12-13 TACATGG 3865 0.0 21.027702 2 CAGAGTA 6780 0.0 20.948223 10-11 GGACCGA 330 2.7284841E-11 20.159357 6 GTACTTT 4850 0.0 20.131058 14-15 ACATGGG 3875 0.0 20.114874 3 >>END_MODULE