FastQCFastQC Report
Thu 26 May 2016
SRR938062_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938062_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1315872
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50270.3820280392013813No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45750.3476781936236959No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.2114187398166387No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27800.050.5825771
GTATCAA45500.037.06561
TCAACGC55550.030.0108384
ATCAACG55150.029.9735583
TATCAAC56100.029.808072
CAACGCA56750.029.2925535
AACGCAG58600.028.3677886
TGGTATC13400.025.1714152
ACGCAGA67600.024.5207527
CGCAGAG67950.024.324558
GTGGTAT14900.023.5938871
TACATGG40200.022.6897262
GTACATG41900.021.9959031
ACATGGG39100.021.9890463
GAGTACT48700.021.60229112-13
GCAGAGT76100.021.5322519
TACCTGG10300.021.2165452
GTACCTG11250.021.114051
CAGAGTA70550.021.07183510-11
GTACTTT50500.020.78528414-15
Produced by FastQC (version 0.11.5)