##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938062_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1315872 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46066106733786 33.0 31.0 34.0 31.0 34.0 2 32.69892360351159 34.0 31.0 34.0 31.0 34.0 3 32.66101110138372 34.0 31.0 34.0 31.0 34.0 4 36.19487305756183 37.0 35.0 37.0 35.0 37.0 5 36.15481141022835 37.0 35.0 37.0 35.0 37.0 6 36.212343601809295 37.0 37.0 37.0 35.0 37.0 7 36.17030075873641 37.0 37.0 37.0 35.0 37.0 8 36.1608173135381 37.0 37.0 37.0 35.0 37.0 9 37.86477332141728 39.0 38.0 39.0 35.0 39.0 10-11 37.824726873130516 39.0 38.0 39.0 35.0 39.0 12-13 37.770308206269306 39.0 38.0 39.0 35.0 39.0 14-15 39.052000498528734 40.0 38.0 41.0 36.0 41.0 16-17 38.98035675202451 40.0 38.0 41.0 36.0 41.0 18-19 38.962047980350675 40.0 38.0 41.0 35.5 41.0 20-21 38.96013404039299 40.0 38.5 41.0 35.0 41.0 22-23 38.9853773771309 40.0 38.5 41.0 35.0 41.0 24-25 38.945124601784975 40.0 38.0 41.0 35.0 41.0 26-27 38.769490877532164 40.0 38.0 41.0 35.0 41.0 28-29 38.69182032902896 40.0 38.0 41.0 34.5 41.0 30-31 38.479570581333135 40.0 38.0 41.0 34.0 41.0 32-33 38.28228049536733 40.0 38.0 41.0 34.0 41.0 34-35 38.08108805415725 40.0 38.0 41.0 33.5 41.0 36-37 38.00303942936699 40.0 38.0 41.0 33.0 41.0 38-39 37.95571301767953 40.0 38.0 41.0 33.0 41.0 40-41 37.83111617239366 40.0 37.5 41.0 33.0 41.0 42-43 37.64049048843657 40.0 37.0 41.0 33.0 41.0 44-45 37.39947198511709 40.0 37.0 41.0 32.5 41.0 46-47 37.065603265363194 40.0 36.5 41.0 31.5 41.0 48-49 36.70000083594757 40.0 36.0 41.0 31.0 41.0 50-51 36.40164164903577 39.0 35.5 41.0 30.0 41.0 52-53 35.925996981469325 39.0 35.0 40.0 29.0 41.0 54-55 35.608345264585004 38.5 35.0 40.0 28.0 41.0 56-57 35.32997396403297 38.0 35.0 40.0 28.0 41.0 58-59 34.94141261460081 38.0 34.0 40.0 26.5 41.0 60-61 34.519401963108876 37.5 34.0 40.0 26.0 41.0 62-63 34.063416502516965 37.0 33.5 40.0 25.5 41.0 64-65 33.61000956020038 36.0 33.0 39.0 24.5 41.0 66-67 33.08394851474915 36.0 32.0 39.0 22.5 40.0 68-69 32.53733607828117 35.0 31.5 38.5 21.5 40.0 70-71 31.937186519661488 35.0 31.0 37.5 19.5 40.0 72-73 31.452225596410592 35.0 31.0 37.0 18.0 39.0 74-75 30.881539769825636 34.0 30.0 36.5 15.5 39.0 76-77 29.709105824882663 33.5 28.5 35.0 14.0 37.5 78-79 30.396486132389775 34.0 30.0 36.0 14.0 37.5 80-81 30.201187501519904 34.0 30.0 35.0 9.5 37.0 82-83 29.74240541633229 34.0 29.5 35.0 7.0 37.0 84-85 29.213581564164294 34.0 29.0 35.0 2.0 36.0 86-87 28.664405428491527 34.0 29.0 35.0 2.0 36.0 88-89 28.036015661097736 33.0 27.0 35.0 2.0 35.0 90-91 27.49713193988473 33.0 26.5 35.0 2.0 35.0 92-93 26.85813665766883 33.0 25.5 35.0 2.0 35.0 94-95 26.097290997908612 32.0 24.5 34.5 2.0 35.0 96-97 25.263603526786802 32.0 21.0 34.0 2.0 35.0 98-99 24.238754225334986 31.5 9.5 34.0 2.0 35.0 100-101 20.81018632511369 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.005871061903995667 1101 2 -0.022041070243567162 1101 3 0.2043878574171103 1101 4 0.038955837187764075 1101 5 0.005603692299651186 1101 6 0.10691687042609033 1101 7 0.021971874355372734 1101 8 0.10905603759075433 1101 9 0.1106516856945845 1101 10-11 0.17473446037177354 1101 12-13 0.11258640601094783 1101 14-15 0.28749379237574857 1101 16-17 0.3368055997965982 1101 18-19 0.3003431530009095 1101 20-21 0.23586272407966646 1101 22-23 0.15227671892054673 1101 24-25 0.17098335972786316 1101 26-27 0.20224957213621764 1101 28-29 0.31622801691792546 1101 30-31 0.38711655336889805 1101 32-33 0.4611659260999019 1101 34-35 0.2829409178025841 1101 36-37 0.42566257889956205 1101 38-39 0.4863608022966517 1101 40-41 0.5976147778777658 1101 42-43 0.405381255976458 1101 44-45 0.5381344020534797 1101 46-47 0.6867874487308043 1101 48-49 0.8863322285598585 1101 50-51 0.94110952281126 1101 52-53 0.8357355430344171 1101 54-55 0.9298241916109902 1101 56-57 0.8833548650479486 1101 58-59 1.0599452173808572 1101 60-61 1.0662498135523606 1101 62-63 1.1268960427860364 1101 64-65 1.106793671995355 1101 66-67 0.9473661578232324 1101 68-69 1.1774946419292291 1101 70-71 1.261517911794222 1101 72-73 1.259405553352412 1101 74-75 1.251942941247421 1101 76-77 1.1104438929652076 1101 78-79 1.2733271918358149 1101 80-81 1.5035117509016196 1101 82-83 1.4188631250559673 1101 84-85 1.3587938882787682 1101 86-87 1.4890185039819919 1101 88-89 1.843295036636004 1101 90-91 1.7686738488860563 1101 92-93 2.0325135763228417 1101 94-95 1.9141729123668476 1101 96-97 2.1462156646523773 1101 98-99 2.5037993906312686 1101 100-101 2.747579550947602 1102 1 -0.016979534747186165 1102 2 0.039617485963098886 1102 3 0.18045214168544277 1102 4 0.005389443148231976 1102 5 -0.033826682621807436 1102 6 -0.03886831937444413 1102 7 0.005694430898557812 1102 8 -0.007996171688034792 1102 9 -0.06994407162783034 1102 10-11 0.09198837955110406 1102 12-13 0.058978171462229056 1102 14-15 0.13502766044734926 1102 16-17 0.12032302874133904 1102 18-19 0.14014349371909418 1102 20-21 0.08061905536500547 1102 22-23 0.14440286877871245 1102 24-25 -0.04946439813087267 1102 26-27 0.019458929793039204 1102 28-29 0.0028372794050071093 1102 30-31 -0.04562742425306965 1102 32-33 0.024616801950720912 1102 34-35 -0.0024960085828311662 1102 36-37 0.14366832589001888 1102 38-39 0.1646450127952903 1102 40-41 0.10461021393658143 1102 42-43 0.041293570993566675 1102 44-45 0.0875605574325462 1102 46-47 0.1275262104199939 1102 48-49 0.16183942807192153 1102 50-51 0.16225593698892027 1102 52-53 0.20253405396972823 1102 54-55 0.30711437782294837 1102 56-57 0.4411248040288953 1102 58-59 0.4747272208353621 1102 60-61 0.6052940543370653 1102 62-63 0.475418085622934 1102 64-65 0.482384868005596 1102 66-67 0.5794693828155388 1102 68-69 0.44421014224076316 1102 70-71 0.5599995995060212 1102 72-73 0.6794849885384018 1102 74-75 0.5611591579610149 1102 76-77 0.7616037413245813 1102 78-79 0.9726401031097502 1102 80-81 1.0647437521214762 1102 82-83 0.8740166848512203 1102 84-85 0.9736840750165214 1102 86-87 1.0951725291099557 1102 88-89 0.9753999047523045 1102 90-91 1.116487376660114 1102 92-93 1.1700874183147825 1102 94-95 0.9232087797917181 1102 96-97 1.317803487657688 1102 98-99 1.297292553920986 1102 100-101 1.6040717309919295 1103 1 0.3020196581503072 1103 2 0.311352756342437 1103 3 0.3922519802649447 1103 4 0.2274233414533171 1103 5 0.10656072657916127 1103 6 0.14758850064169593 1103 7 0.24109394663705785 1103 8 0.22904983315457628 1103 9 0.28978151352148274 1103 10-11 0.34222243927668927 1103 12-13 0.18309439422251472 1103 14-15 0.5353020752980413 1103 16-17 0.4765457890318956 1103 18-19 0.534001443678747 1103 20-21 0.43965053236258456 1103 22-23 0.5050485507803302 1103 24-25 0.4928439150248991 1103 26-27 0.4719044503741614 1103 28-29 0.4978574569675587 1103 30-31 0.544711558797772 1103 32-33 0.5589509423865522 1103 34-35 0.4860431358885151 1103 36-37 0.5267514574477232 1103 38-39 0.5393342783320136 1103 40-41 0.5187587207969528 1103 42-43 0.6304138292663666 1103 44-45 0.6481739553340802 1103 46-47 0.8832889222844003 1103 48-49 0.9520977008725637 1103 50-51 1.1289387457056321 1103 52-53 1.084915006350684 1103 54-55 1.130011875805188 1103 56-57 1.0235541825599697 1103 58-59 1.1607804896004907 1103 60-61 1.1530987355315787 1103 62-63 1.2457489976487608 1103 64-65 1.1794228018231863 1103 66-67 1.2248285434289414 1103 68-69 1.365202878318243 1103 70-71 1.636085152371237 1103 72-73 1.5668376923317773 1103 74-75 1.2233141216414012 1103 76-77 0.8719507954004477 1103 78-79 1.3725593928595465 1103 80-81 1.5255266415484314 1103 82-83 1.6378262086607442 1103 84-85 1.7655807658962637 1103 86-87 2.040886814824244 1103 88-89 2.3408559176659445 1103 90-91 2.228674624440579 1103 92-93 2.5040018654495704 1103 94-95 2.4483903778546754 1103 96-97 2.2769237459304854 1103 98-99 2.890763094679663 1103 100-101 3.0476229820008065 1104 1 0.019279276916577714 1104 2 0.04803841435749234 1104 3 0.04662632084312435 1104 4 0.04364335974128153 1104 5 -0.07802955266399891 1104 6 -0.01535445497481902 1104 7 0.004371712691778384 1104 8 0.028051028906141084 1104 9 -0.08375720772772866 1104 10-11 -0.02929546182762266 1104 12-13 -0.03633234840954458 1104 14-15 0.11154389846748103 1104 16-17 0.11144907383178548 1104 18-19 0.03171755818954125 1104 20-21 0.11741166340289055 1104 22-23 0.04805903119110155 1104 24-25 0.06224955615875416 1104 26-27 0.09682602313916533 1104 28-29 0.15276074176744459 1104 30-31 0.07045257307645869 1104 32-33 0.20079732347961254 1104 34-35 0.20346593957424375 1104 36-37 0.06227793982797181 1104 38-39 0.016283001691370202 1104 40-41 0.13354102367378573 1104 42-43 0.25403273620876377 1104 44-45 0.3601174033188883 1104 46-47 0.1683230363731596 1104 48-49 3.3994607196774496E-4 1104 50-51 0.30572287737897597 1104 52-53 0.2775498800933889 1104 54-55 0.40607503962315405 1104 56-57 0.5275536198533501 1104 58-59 0.5189742013540339 1104 60-61 0.7243908506050829 1104 62-63 0.5072619750701648 1104 64-65 0.47624069589367224 1104 66-67 0.5297592601764975 1104 68-69 0.6482562651087136 1104 70-71 0.8726814807105399 1104 72-73 0.839624496861564 1104 74-75 0.8453349417862981 1104 76-77 0.7987525116556391 1104 78-79 0.9302719904539778 1104 80-81 0.9915722207845583 1104 82-83 1.063138862525836 1104 84-85 1.14447715754007 1104 86-87 1.0823533689495868 1104 88-89 1.4393014406314073 1104 90-91 1.284892532578727 1104 92-93 1.6665073166699393 1104 94-95 1.5729701063487305 1104 96-97 1.7932387209603817 1104 98-99 1.8315079775663499 1104 100-101 2.00405331188756 1105 1 0.06749006706442628 1105 2 0.025369365193483873 1105 3 0.14993725304955774 1105 4 0.11634376613532993 1105 5 -0.004022685593653819 1105 6 0.0707105441610949 1105 7 0.08170994579609925 1105 8 0.11784411466409495 1105 9 0.17384542606221487 1105 10-11 0.04171996897289176 1105 12-13 0.11395111660241497 1105 14-15 0.14951630845266806 1105 16-17 0.20448439935461238 1105 18-19 0.22044841098663426 1105 20-21 0.18218390774364224 1105 22-23 0.08207157190931014 1105 24-25 0.1290638246222926 1105 26-27 0.18478577067269697 1105 28-29 0.056134551469817495 1105 30-31 0.17655114673007688 1105 32-33 0.19048883789795212 1105 34-35 0.264178319321708 1105 36-37 0.3544735219909825 1105 38-39 0.25913034686980296 1105 40-41 0.3529109335584337 1105 42-43 0.07226813390212783 1105 44-45 0.25689885809871527 1105 46-47 0.33849443053626516 1105 48-49 0.25597555223385626 1105 50-51 0.1992908953955279 1105 52-53 0.32200215025902423 1105 54-55 0.3184182670592648 1105 56-57 0.3507159611787003 1105 58-59 0.4363150212939715 1105 60-61 0.4004013740262735 1105 62-63 0.5069725864220231 1105 64-65 0.7057856736827475 1105 66-67 0.5950377308566672 1105 68-69 0.586072215015399 1105 70-71 0.7000933516774523 1105 72-73 0.7546007534060983 1105 74-75 0.6342812192972396 1105 76-77 0.7383189232255312 1105 78-79 0.9624889629215687 1105 80-81 1.0683659141228041 1105 82-83 0.9750889018174753 1105 84-85 1.2504730186030883 1105 86-87 1.3800984199961128 1105 88-89 1.6391214490440582 1105 90-91 1.365487406948727 1105 92-93 1.6994459945360845 1105 94-95 1.5833215247063528 1105 96-97 1.420364300960447 1105 98-99 1.8738630631438689 1105 100-101 2.0867252630898427 1106 1 0.02297203910269019 1106 2 0.05688070665299705 1106 3 0.1787116075941384 1106 4 0.039121064020605445 1106 5 0.04283401850462809 1106 6 0.0068224550930438 1106 7 -0.020748703466466623 1106 8 -0.08285492875018008 1106 9 0.0036531491322477905 1106 10-11 0.01508993410070758 1106 12-13 -0.0030546741004684463 1106 14-15 -0.018279705240267674 1106 16-17 0.0299722900670929 1106 18-19 0.03745900061456098 1106 20-21 -0.007564788548393153 1106 22-23 -0.12943385087598358 1106 24-25 -0.0033569131324568957 1106 26-27 0.06877815637830054 1106 28-29 -0.009875462287105563 1106 30-31 0.16710286314560108 1106 32-33 0.17177702934307604 1106 34-35 -0.0822280856435782 1106 36-37 0.21292122970445604 1106 38-39 0.19574214582683425 1106 40-41 0.09849992162510546 1106 42-43 0.11092107564317644 1106 44-45 0.2687226095783828 1106 46-47 -0.10952681270524778 1106 48-49 -0.030221139707144573 1106 50-51 0.2144666960161885 1106 52-53 0.08431459227200833 1106 54-55 0.21828309733728446 1106 56-57 0.06235335440262446 1106 58-59 0.091339495811674 1106 60-61 -0.028702506704448183 1106 62-63 0.12425831441895241 1106 64-65 0.18975406476315015 1106 66-67 0.35433164901900227 1106 68-69 -4.6999952854775984E-4 1106 70-71 0.12482221241263858 1106 72-73 0.266765228563667 1106 74-75 0.37404911128943397 1106 76-77 0.42580172707746655 1106 78-79 0.5277871361307724 1106 80-81 0.726416910699573 1106 82-83 0.710145049240456 1106 84-85 0.5950942234532022 1106 86-87 0.8634437506420483 1106 88-89 1.0311768286390297 1106 90-91 1.0561590964902692 1106 92-93 0.9572834389485365 1106 94-95 1.295181268123823 1106 96-97 1.1412612200505698 1106 98-99 1.3189825563773852 1106 100-101 1.653999793595009 1107 1 0.06617317224716857 1107 2 0.09253986007912118 1107 3 0.18135355857332058 1107 4 0.09610673549082094 1107 5 0.10190943830180998 1107 6 0.03773553962726339 1107 7 0.03241055259955061 1107 8 0.015823069361246667 1107 9 -0.02600157025027272 1107 10-11 0.041678799724550686 1107 12-13 0.08712987243646353 1107 14-15 0.07196334603862908 1107 16-17 0.06289814300465224 1107 18-19 0.12366419829901076 1107 20-21 0.13936319929904784 1107 22-23 0.03126533211042215 1107 24-25 0.13886914097930259 1107 26-27 0.034313731367006994 1107 28-29 0.03161774295163866 1107 30-31 0.02575027456737189 1107 32-33 0.08138720300977553 1107 34-35 0.16004826201828593 1107 36-37 0.2937142255577996 1107 38-39 0.1836645090078548 1107 40-41 -0.03612256882938425 1107 42-43 0.14963479243916566 1107 44-45 0.17897120341178407 1107 46-47 0.2314603778354538 1107 48-49 0.09194189350711923 1107 50-51 0.15010224226572433 1107 52-53 0.062155664653666065 1107 54-55 0.0888254465592766 1107 56-57 0.17263889859935944 1107 58-59 0.21439036414521695 1107 60-61 0.37941938843863454 1107 62-63 0.3673455172764477 1107 64-65 0.2072172170396982 1107 66-67 0.4630991491216463 1107 68-69 0.4055549624946906 1107 70-71 0.4311458565395867 1107 72-73 0.3467432636254557 1107 74-75 0.45487084631427166 1107 76-77 0.6163875956162848 1107 78-79 0.7893946822160842 1107 80-81 0.8873275318762346 1107 82-83 1.0796014683396855 1107 84-85 1.3260360471589117 1107 86-87 1.3062712745571439 1107 88-89 1.3834533815806864 1107 90-91 1.2876757036257942 1107 92-93 1.7615912354752012 1107 94-95 1.8121230097176166 1107 96-97 1.7466795802755577 1107 98-99 1.8261273196897783 1107 100-101 2.0202528214720026 1108 1 -0.043375430117556846 1108 2 -0.04172652188047721 1108 3 0.054991866917276866 1108 4 0.002656225304534132 1108 5 -0.08567170127570023 1108 6 -0.15175225891793787 1108 7 -0.12516492900622467 1108 8 -0.07795296796587081 1108 9 -0.1676349414325955 1108 10-11 -0.05917551525396192 1108 12-13 -0.10939599728293103 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quality scores pass #Quality Count 2 4.0 3 1.0 4 7.0 5 22.0 6 58.0 7 136.0 8 274.0 9 526.0 10 947.0 11 1574.0 12 2454.0 13 3523.0 14 4930.0 15 6765.0 16 8556.0 17 11615.0 18 11801.0 19 9362.0 20 8853.0 21 8981.0 22 9549.0 23 10268.0 24 11418.0 25 13681.0 26 16007.0 27 19076.0 28 22776.0 29 27757.0 30 33567.0 31 41258.0 32 51369.0 33 66958.0 34 91164.0 35 133262.0 36 202467.0 37 282445.0 38 188154.0 39 14306.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.468133678655676 18.598237518542838 14.644509496364389 23.2891193064371 2 16.126884525231887 19.579833337893586 39.790349893138035 24.502932243736492 3 20.065466970871988 23.09295337055339 29.421680557054458 27.41989910152017 4 13.261092264293184 16.49476544831108 36.07334148002238 34.17080080737336 5 14.003760255587947 36.724276295190705 34.26969878437655 15.002264664844803 6 33.21559093738197 35.49819244650105 16.746297308162603 14.539919307954374 7 29.276783760122566 32.04407419566645 19.935601639065197 18.74354040514579 8 26.618531175975725 35.16740648195223 19.01389879995653 19.200163542115515 9 25.86345784392403 16.891384572359623 19.92389837309404 37.32125921062231 10-11 25.39274336713601 26.23959625252304 26.7173782860339 21.650282094307045 12-13 26.16109317623599 23.439551871306634 27.33092580433355 23.06842914812383 14-15 22.79929202840398 25.10855919116753 25.524519102161914 26.56762967826658 16-17 22.421329734199073 28.212318523382212 26.6098070329029 22.75654470951582 18-19 22.39967109262907 26.674212993361056 28.836885350550812 22.089230563459058 20-21 22.76007848787724 25.85259052552224 29.304256037061354 22.083074949539164 22-23 22.551889545487708 25.743271382018918 29.212757775832298 22.492081296661073 24-25 22.550496692830926 25.89501794842663 29.257968307262168 22.296517051480276 26-27 22.120298711560306 26.24291806639411 29.07424728266963 22.562535939375948 28-29 22.25254442411565 26.57022838545674 28.99053286140132 22.186694329026288 30-31 22.36357335667907 26.18195386785341 28.898023515965075 22.556449259502447 32-33 22.059885827892177 26.175682777400112 28.94311952124169 22.821311873466023 34-35 22.70124705565804 26.35025489127502 28.792947108635293 22.155550944431653 36-37 22.721310079976163 25.96538748881831 28.7386368559175 22.574665575288027 38-39 22.28263038376391 26.288901346356663 28.829971400609995 22.598496869269432 40-41 22.77136205995534 26.056610765357025 28.783695187419827 22.388331987267804 42-43 22.458127140864057 26.11400940773529 28.6466185701869 22.781244881213755 44-45 22.504490789843196 26.41257048744498 28.714112157452583 22.36882656525924 46-47 22.867265622268867 26.58058009827898 28.081325626251097 22.470828653201057 48-49 22.65735875984757 26.58681078419191 28.02635213723539 22.729478318725132 50-51 22.69498857031687 26.880122078743224 27.982052965637994 22.442836385301916 52-53 22.972371172880038 26.650578475718 27.898800187252252 22.478250164149706 54-55 22.654217127501763 26.768979049634005 27.989576493762314 22.587227329101918 56-57 22.64593364704166 26.96272889764354 27.827820639089516 22.563516816225288 58-59 22.64156392111087 26.634847462367162 27.80000638359962 22.923582232922353 60-61 22.366955144573332 26.87955211449138 27.981900975170838 22.77159176576445 62-63 22.41559209406386 26.816210087303322 28.137995184942 22.630202633690814 64-65 22.308134833783225 26.91059616740838 27.95199685075752 22.829272148050876 66-67 22.270192302942586 26.87411678920316 27.832686674522073 23.02300423333218 68-69 22.661303407298536 26.60528252676088 27.92923902234763 22.80417504359295 70-71 22.479656075970915 26.79531139806911 27.600594890688456 23.124437635271516 72-73 22.520845492570707 26.855689611147586 27.56062139782593 23.062843498455777 74-75 22.430981128863596 27.195350307628708 27.3773208944335 22.996347669074197 76-77 22.576018032149022 26.87936212640743 27.59432528391819 22.950294557525353 78-79 22.552117531188443 27.086829114077965 27.546372291529874 22.814681063203714 80-81 22.579696443716237 27.04948368681357 27.407612313697115 22.96320755577307 82-83 22.520313834686576 27.163579186717048 27.43325237184729 22.88285460674909 84-85 22.522714231972063 27.196469081664837 27.344971329338314 22.935845357024785 86-87 22.701149097718336 27.172222545205276 27.22321587009546 22.903412486980933 88-89 22.618727353420393 27.267051810510445 26.980321794217065 23.133899041852096 90-91 22.497793190296346 27.218256994627275 27.131694575257054 23.152255239819322 92-93 22.60583524462217 27.041241281407075 27.19885611452838 23.154067359442372 94-95 22.45609368706635 26.91033308761504 27.279974465562706 23.353598759755904 96-97 22.626027884984033 26.661952420053815 27.237883391932105 23.47413630303005 98-99 22.390767176557265 26.929093706409557 27.018920174895626 23.661218942137545 100-101 22.77295744077724 26.530147731063952 27.09328739045946 23.60360743769935 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 531.0 1 380.0 2 339.0 3 476.0 4 355.5 5 282.5 6 564.0 7 1207.0 8 1903.5 9 2193.0 10 1791.0 11 1233.0 12 1157.0 13 1241.5 14 1326.0 15 1395.0 16 1445.5 17 1576.0 18 1762.5 19 1950.0 20 2222.5 21 2570.5 22 3069.5 23 3789.5 24 4611.0 25 5782.5 26 7383.0 27 9290.5 28 10985.0 29 12545.0 30 14314.5 31 16790.5 32 20378.0 33 22333.5 34 22982.0 35 24586.0 36 27022.0 37 29391.0 38 31424.5 39 33108.5 40 34881.5 41 37488.0 42 39106.0 43 40393.0 44 42642.0 45 45696.5 46 47513.5 47 48665.0 48 49533.0 49 49311.5 50 49055.0 51 48498.5 52 48303.5 53 48950.0 54 49218.5 55 46301.0 56 42623.5 57 39382.5 58 35965.0 59 31728.0 60 25829.0 61 21132.5 62 17574.0 63 13208.0 64 9276.5 65 6931.0 66 5145.5 67 3823.0 68 2907.5 69 2249.5 70 1678.5 71 1097.5 72 733.5 73 530.5 74 372.5 75 264.5 76 189.0 77 115.0 78 54.5 79 27.0 80 20.5 81 14.5 82 7.5 83 5.0 84 4.0 85 2.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011855256438316189 3 0.0071435519564212934 4 0.0 5 3.039809343157997E-4 6 3.799761678947496E-4 7 0.0 8 2.2798570073684978E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0014059118212105736 26-27 0.021924624887527056 28-29 3.419785511052747E-4 30-31 0.0 32-33 0.003913754529315921 34-35 0.016186984752316332 36-37 0.007979499525789743 38-39 0.021620643953211255 40-41 0.023520524792685002 42-43 0.019188796478684857 44-45 0.009385411347000315 46-47 0.001975876073052698 48-49 1.1399285036842489E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.179737846842246E-4 68-69 4.179737846842246E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.039809343157997E-4 82-83 0.0015199046715789984 84-85 0.005091680649789645 86-87 0.001025935653315824 88-89 0.0 90-91 0.0044457211643685705 92-93 4.5597140147369956E-4 94-95 1.5199046715789986E-4 96-97 1.899880839473748E-4 98-99 1.1399285036842489E-4 100-101 0.0026598331752632474 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1315872.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.32691100070963 #Duplication Level Percentage of deduplicated Percentage of total 1 83.86256883004219 56.46207707911135 2 10.045539286193815 13.52670258951406 3 2.6795850976569904 5.412245621663399 4 1.1191295321159236 3.0139013762813827 5 0.5860707837906611 1.972916775019499 6 0.37638443412734535 1.520448077912654 7 0.2550510835272067 1.2020261120889562 8 0.18187694705037202 0.9796170421712939 9 0.13112466509587675 0.7945396791217156 >10 0.7029868711566004 8.491798298147712 >50 0.03673920838322153 1.69555985498809 >100 0.02000474316046261 2.663112662834319 >500 0.0020343584072095174 0.8808504291779848 >1k 7.91139380581479E-4 1.0016858197735825 >5k 1.1301991151163987E-4 0.3825185821939988 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5027 0.3820280392013813 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4575 0.3476781936236959 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2782 0.2114187398166387 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.7997616789474965E-5 6.079618686315994E-4 14-15 0.0 0.0 0.0 7.599523357894993E-5 7.599523357894992E-4 16-17 0.0 0.0 0.0 7.599523357894993E-5 9.119428029473991E-4 18-19 0.0 0.0 0.0 7.599523357894993E-5 9.87938036526349E-4 20-21 0.0 0.0 0.0 3.799761678947496E-4 0.0010259356533158241 22-23 0.0 0.0 0.0 5.699642518421245E-4 0.001139928503684249 24-25 0.0 0.0 0.0 9.499404197368741E-4 0.0012159237372631989 26-27 0.0 0.0 0.0 0.0014059118212105736 0.0012919189708421488 28-29 7.599523357894993E-5 0.0 0.0 0.0036477712117895965 0.0012919189708421488 30-31 7.599523357894993E-5 0.0 0.0 0.011969249288684614 0.0012919189708421488 32-33 7.599523357894993E-5 0.0 0.0 0.029296162544685197 0.0012919189708421488 34-35 7.599523357894993E-5 0.0 0.0 0.05521053719510712 0.0013679142044210988 36-37 7.599523357894993E-5 0.0 0.0 0.0989457941197928 0.0013679142044210988 38-39 7.599523357894993E-5 0.0 0.0 0.16787347097590039 0.0013679142044210988 40-41 7.599523357894993E-5 0.0 0.0 0.2347872741421658 0.0013679142044210988 42-43 7.599523357894993E-5 0.0 0.0 0.30584281753848397 0.0013679142044210988 44-45 7.599523357894993E-5 0.0 0.0 0.3911854648476447 0.0014059118212105736 46-47 7.599523357894993E-5 0.0 0.0 0.4885733566790691 0.0014819070547895238 48-49 7.599523357894993E-5 0.0 0.0 0.5897230125726514 0.0015199046715789987 50-51 7.599523357894993E-5 0.0 0.0 0.6971042776197076 0.0016718951387368983 52-53 7.599523357894993E-5 0.0 0.0 0.7980259478125532 0.0018238856058947982 54-55 7.599523357894993E-5 0.0 0.0 0.9118288100970308 0.0018618832226842733 56-57 7.599523357894993E-5 0.0 0.0 1.0439085260572458 0.0018998808394737482 58-59 7.599523357894993E-5 0.0 0.0 1.1860196128498819 0.0018998808394737482 60-61 7.599523357894993E-5 0.0 0.0 1.3283966829600447 0.0018998808394737482 62-63 7.599523357894993E-5 0.0 0.0 1.4665180199897863 0.002127866540210598 64-65 7.599523357894993E-5 0.0 0.0 1.6091610734174753 0.0021658641570000726 66-67 7.599523357894993E-5 0.0 0.0 1.7740327326670071 0.0022038617737895477 68-69 7.599523357894993E-5 0.0 0.0 1.9603730454025923 0.002279857007368498 70-71 7.599523357894993E-5 0.0 0.0 2.1553768147661776 0.002279857007368498 72-73 7.599523357894993E-5 0.0 0.0 2.349126662775711 0.0023938498577369227 74-75 7.599523357894993E-5 0.0 0.0 2.5423065465334016 0.0025078427081053475 76-77 7.599523357894993E-5 0.0 0.0 2.750305500838987 0.0025078427081053475 78-79 7.599523357894993E-5 0.0 0.0 2.9883605700250477 0.0025078427081053475 80-81 7.599523357894993E-5 0.0 0.0 3.2377389290143723 0.0025458403248948226 82-83 7.599523357894993E-5 0.0 0.0 3.4821775978210647 0.0028118236424211473 84-85 7.599523357894993E-5 0.0 0.0 3.7301500449891782 0.0028118236424211473 86-87 7.599523357894993E-5 0.0 0.0 3.989559774810924 0.0029258164927895725 88-89 7.599523357894993E-5 0.0 0.0 4.269564212932565 0.0030398093431579973 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2780 0.0 50.582577 1 GTATCAA 4550 0.0 37.0656 1 TCAACGC 5555 0.0 30.010838 4 ATCAACG 5515 0.0 29.973558 3 TATCAAC 5610 0.0 29.80807 2 CAACGCA 5675 0.0 29.292553 5 AACGCAG 5860 0.0 28.367788 6 TGGTATC 1340 0.0 25.171415 2 ACGCAGA 6760 0.0 24.520752 7 CGCAGAG 6795 0.0 24.32455 8 GTGGTAT 1490 0.0 23.593887 1 TACATGG 4020 0.0 22.689726 2 GTACATG 4190 0.0 21.995903 1 ACATGGG 3910 0.0 21.989046 3 GAGTACT 4870 0.0 21.602291 12-13 GCAGAGT 7610 0.0 21.532251 9 TACCTGG 1030 0.0 21.216545 2 GTACCTG 1125 0.0 21.11405 1 CAGAGTA 7055 0.0 21.071835 10-11 GTACTTT 5050 0.0 20.785284 14-15 >>END_MODULE