FastQCFastQC Report
Thu 26 May 2016
SRR938061_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938061_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338077
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52850.39496979620754263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45680.34138543596519483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26680.19939061802870836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23250.050.7456671
GTATCAA40100.039.309231
ATCAACG50900.030.1596553
TCAACGC51000.030.1005174
TATCAAC51300.030.0171382
CAACGCA52050.029.310685
TGGTATC11200.028.855762
AACGCAG53400.028.6586846
GTGGTAT11650.028.2909891
CGCAGAG61950.024.6386368
ACGCAGA62000.024.5420727
TACATGG39000.024.0072962
TATACCG1000.00164829823.7635675
GTACATG40900.023.3577841
GGACCGT1852.5283225E-723.1213076
GACCGCG1252.2400692E-422.824137
ACATGGG39200.022.551143
GCAGAGT69150.021.6598329
GTATAGC6100.021.1426241
AGAGTAC62900.020.06939710-11
Produced by FastQC (version 0.11.5)