FastQCFastQC Report
Thu 26 May 2016
SRR938061_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938061_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1338077
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51260.38308707196969977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46820.34990512504138405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29640.22151191598091888No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.051.59811
GTATCAA42650.039.9901581
ATCAACG53550.031.2245313
TCAACGC54150.030.7873784
TATCAAC55200.030.4678542
CAACGCA55100.030.2565635
AACGCAG56800.029.3509946
GGACCGA3550.025.4208056
ACGCAGA66550.024.8367797
AACCGAC1151.2824996E-424.7809227
CGCAGAG67050.024.6515678
TGGTATC12050.024.4445672
GTGGTAT14200.022.416311
GCAGAGT73550.022.2792459
GAGTACT47650.022.02893312-13
AGGACCG4700.021.221965
GTACTTT49700.020.9291614-15
AGAGTAC65700.020.85664710-11
GTACCGG1150.003719419720.6561721
GTACCTG10900.020.4856171
Produced by FastQC (version 0.11.5)