##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938061_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1338077 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.475288791302745 34.0 31.0 34.0 31.0 34.0 2 32.699008353032 34.0 31.0 34.0 31.0 34.0 3 32.75729872047722 34.0 31.0 34.0 31.0 34.0 4 36.222236089552396 37.0 35.0 37.0 35.0 37.0 5 36.18185276333126 37.0 35.0 37.0 35.0 37.0 6 36.2150907608456 37.0 37.0 37.0 35.0 37.0 7 36.17714003005806 37.0 37.0 37.0 35.0 37.0 8 36.16646874581956 37.0 37.0 37.0 35.0 37.0 9 37.869921536652974 39.0 38.0 39.0 35.0 39.0 10-11 37.8585118793612 39.0 38.0 39.0 35.0 39.0 12-13 37.808997912676176 39.0 38.0 39.0 35.0 39.0 14-15 39.13616219395446 40.5 38.5 41.0 36.0 41.0 16-17 39.08291600558114 40.0 38.0 41.0 36.0 41.0 18-19 39.10078231671272 40.0 38.5 41.0 36.0 41.0 20-21 39.020729375065855 40.0 38.5 41.0 35.0 41.0 22-23 38.88193728761499 40.0 38.0 41.0 35.0 41.0 24-25 38.84768514816412 40.0 38.0 41.0 35.0 41.0 26-27 38.81067681456299 40.0 38.0 41.0 35.0 41.0 28-29 38.75613511031129 40.0 38.0 41.0 35.0 41.0 30-31 38.5652312983483 40.0 38.0 41.0 34.0 41.0 32-33 38.397923662091195 40.0 38.0 41.0 34.0 41.0 34-35 38.20794655315053 40.0 38.0 41.0 34.0 41.0 36-37 38.08620430662809 40.0 38.0 41.0 33.5 41.0 38-39 38.06990031216439 40.0 38.0 41.0 33.0 41.0 40-41 38.009229289495295 40.0 38.0 41.0 33.0 41.0 42-43 37.86116494043317 40.0 37.5 41.0 33.0 41.0 44-45 37.673420513169276 40.0 37.0 41.0 33.0 41.0 46-47 37.37415933462723 40.0 37.0 41.0 32.5 41.0 48-49 37.05390945364131 40.0 37.0 41.0 31.5 41.0 50-51 36.760609815429156 40.0 36.0 41.0 31.0 41.0 52-53 36.34494464817794 39.0 35.5 41.0 30.0 41.0 54-55 36.12687760121428 39.0 35.0 41.0 30.0 41.0 56-57 35.88169178604819 39.0 35.0 40.5 29.0 41.0 58-59 35.542188155091225 38.5 35.0 40.0 28.0 41.0 60-61 35.18016115664494 38.0 34.5 40.0 28.0 41.0 62-63 34.810654020658006 37.5 34.0 40.0 27.0 41.0 64-65 34.413238550546794 37.0 34.0 40.0 26.0 41.0 66-67 33.98716553681141 36.0 33.5 39.0 26.0 41.0 68-69 33.5260171873517 36.0 33.0 39.0 25.5 40.5 70-71 33.00776786388227 35.0 32.5 38.5 24.5 40.0 72-73 32.59404877297794 35.0 32.0 37.5 23.5 40.0 74-75 32.14050611437159 35.0 32.0 37.0 22.0 39.0 76-77 30.86967977179191 34.0 30.5 35.5 21.0 38.5 78-79 31.57727843763849 35.0 31.5 36.0 22.0 38.5 80-81 31.41417945305091 35.0 32.0 36.0 21.5 37.5 82-83 31.047008879160167 34.5 31.0 35.0 20.0 37.0 84-85 30.660904043638745 34.0 31.0 35.0 18.5 36.5 86-87 30.228368397334386 34.0 31.0 35.0 15.5 36.0 88-89 29.762406797217203 34.0 30.0 35.0 8.5 36.0 90-91 29.407254963653063 34.0 30.0 35.0 4.5 35.0 92-93 28.996652285331862 34.0 29.0 35.0 2.0 35.0 94-95 28.57257953017651 34.0 29.0 35.0 2.0 35.0 96-97 27.981896408054247 34.0 29.0 35.0 2.0 35.0 98-99 27.27351191299155 33.5 27.0 35.0 2.0 35.0 100-101 24.855964193390964 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1305276697291191 1101 2 0.17532616689390323 1101 3 0.08863414928025293 1101 4 -0.01700153786967462 1101 5 0.042765548190239144 1101 6 0.08686373786592583 1101 7 0.10606334262905648 1101 8 0.08087571825719664 1101 9 0.004910299981361277 1101 10-11 0.12751188897711785 1101 12-13 0.21696612134256554 1101 14-15 0.17623207968971144 1101 16-17 0.2199545507705949 1101 18-19 0.22693595817832346 1101 20-21 0.2629372691600693 1101 22-23 0.358300231362513 1101 24-25 0.22264117923577942 1101 26-27 0.24527178135424066 1101 28-29 0.26918893749262907 1101 30-31 0.11594801546453226 1101 32-33 0.29211086216338344 1101 34-35 0.21850941208163732 1101 36-37 0.3669932541014589 1101 38-39 0.4039216677206525 1101 40-41 0.41752102621602916 1101 42-43 0.3786515974774787 1101 44-45 0.4727833324856192 1101 46-47 0.31019576522953685 1101 48-49 0.355469053406118 1101 50-51 0.25464690750477104 1101 52-53 0.16235464048472892 1101 54-55 0.1988899373335471 1101 56-57 0.275689615906451 1101 58-59 0.3778689344538222 1101 60-61 0.37672469942756237 1101 62-63 0.18580876226855025 1101 64-65 0.32338364681679366 1101 66-67 -0.15568128246129476 1101 68-69 -0.016172369618701055 1101 70-71 0.1363102374686278 1101 72-73 0.29854363913818815 1101 74-75 0.1296620416277321 1101 76-77 -0.004389253684102812 1101 78-79 0.25525919779258643 1101 80-81 0.2849582080492432 1101 82-83 0.4251703365628714 1101 84-85 0.3987346459903911 1101 86-87 0.4511510372012779 1101 88-89 0.24979983253172122 1101 90-91 0.5037384300915768 1101 92-93 0.7174465395594076 1101 94-95 0.7711715037320594 1101 96-97 0.8372572693522429 1101 98-99 0.997457017703546 1101 100-101 1.4076205527405214 1102 1 0.1351000877279276 1102 2 0.14956385011616646 1102 3 0.1535201966044255 1102 4 0.07550467321835441 1102 5 0.10633588916510917 1102 6 0.07872131410405103 1102 7 0.09646403677535886 1102 8 0.12345341970981849 1102 9 0.09681506372795212 1102 10-11 0.15555912547807793 1102 12-13 0.12386168610355242 1102 14-15 0.24928923717092744 1102 16-17 0.2730769198413725 1102 18-19 0.2754336935647004 1102 20-21 0.20363056453711437 1102 22-23 0.14870550243927738 1102 24-25 0.04675489855261361 1102 26-27 0.10034327816521937 1102 28-29 0.14205953670952454 1102 30-31 0.17006635039011542 1102 32-33 0.07515597780501082 1102 34-35 0.07193256928695035 1102 36-37 0.07333417224660366 1102 38-39 0.05060133029128622 1102 40-41 0.1657344117392583 1102 42-43 -0.02303142365762767 1102 44-45 0.22057899252411772 1102 46-47 0.10265582023006203 1102 48-49 0.20358103735720334 1102 50-51 0.006360355557895048 1102 52-53 0.10346920685602612 1102 54-55 0.1234282797627273 1102 56-57 0.10513255834413826 1102 58-59 0.13297350119901097 1102 60-61 -0.04309521447456888 1102 62-63 -0.003049893093738376 1102 64-65 -0.07575982254807201 1102 66-67 -0.14107990731931608 1102 68-69 0.10596366449038896 1102 70-71 0.19715633785940412 1102 72-73 -0.017464400788114176 1102 74-75 -0.026361972430528624 1102 76-77 0.0075552455545810915 1102 78-79 0.23257438327430435 1102 80-81 0.0748674301128851 1102 82-83 0.3044766801884933 1102 84-85 0.2335906145011144 1102 86-87 0.12551210415468006 1102 88-89 0.09396920990157298 1102 90-91 -0.031438826045057766 1102 92-93 0.1665636432262403 1102 94-95 0.16785761268362975 1102 96-97 0.2337100193138859 1102 98-99 0.14397358683647 1102 100-101 0.2114742637286362 1103 1 0.11874521979602548 1103 2 0.1590756849361341 1103 3 0.20237618280183511 1103 4 0.18526196261795036 1103 5 0.1497828533091976 1103 6 0.14072960618372576 1103 7 0.13483338640216402 1103 8 0.15336216673874503 1103 9 0.0730307780834707 1103 10-11 0.0482156607219153 1103 12-13 0.15125543208625203 1103 14-15 0.2677190044589395 1103 16-17 0.24064471429603174 1103 18-19 0.1377510905182575 1103 20-21 0.11648447834108566 1103 22-23 0.22436133226745625 1103 24-25 0.1987700944338897 1103 26-27 0.12308698173526977 1103 28-29 0.19722980751145514 1103 30-31 0.08955373998880845 1103 32-33 0.12732666584214059 1103 34-35 0.21787436184462905 1103 36-37 0.1589576005667297 1103 38-39 0.10778980407501138 1103 40-41 0.31162639919464397 1103 42-43 0.4027097952956282 1103 44-45 0.16905921645286526 1103 46-47 0.2188335824711558 1103 48-49 0.20998584821685284 1103 50-51 0.24911856838794222 1103 52-53 0.18837468992792594 1103 54-55 0.17574462423109338 1103 56-57 0.2743004435129919 1103 58-59 0.37490914674204845 1103 60-61 0.32873871589351467 1103 62-63 0.30168842024863807 1103 64-65 0.31253109187360906 1103 66-67 0.37590112664714326 1103 68-69 0.3380892710789425 1103 70-71 0.37092727949534066 1103 72-73 0.3252611098238205 1103 74-75 0.2801802879818638 1103 76-77 0.23052830318691164 1103 78-79 0.21300567666582282 1103 80-81 0.478841313444903 1103 82-83 0.46296716584775766 1103 84-85 0.5917218769445576 1103 86-87 0.6348052977079561 1103 88-89 0.5254796764908036 1103 90-91 0.4312803320670362 1103 92-93 0.5167536543901008 1103 94-95 0.4025815420618777 1103 96-97 0.3008836330092244 1103 98-99 0.5958217633432454 1103 100-101 0.733741529810672 1104 1 0.07083655569017822 1104 2 0.05196941092845009 1104 3 0.030011692831706682 1104 4 0.01310020418858926 1104 5 0.033799071832454786 1104 6 0.01778893183460184 1104 7 0.06729888647329574 1104 8 0.05490221510977733 1104 9 0.019146868565755426 1104 10-11 0.04898568490744282 1104 12-13 0.0334790769859552 1104 14-15 0.07730073728010467 1104 16-17 0.03324323471145618 1104 18-19 0.11289335826615599 1104 20-21 0.05338339710620943 1104 22-23 0.0784693134881067 1104 24-25 0.0956722142233346 1104 26-27 0.01781240498181802 1104 28-29 0.0785287120497884 1104 30-31 0.08286537749059164 1104 32-33 0.12244866043597114 1104 34-35 0.14407118927384488 1104 36-37 0.265439674830489 1104 38-39 0.21245262951248378 1104 40-41 0.12909510029010818 1104 42-43 0.14155600386014555 1104 44-45 0.2029509506967173 1104 46-47 0.19575241872097848 1104 48-49 0.14889607726792065 1104 50-51 0.28591612138267664 1104 52-53 0.24490477955661305 1104 54-55 0.25829192825727176 1104 56-57 0.3132426828073491 1104 58-59 0.25095303634227406 1104 60-61 0.11956066780701491 1104 62-63 0.14625379227879876 1104 64-65 0.04417855239430679 1104 66-67 0.0528679782618795 1104 68-69 -0.025616448084520016 1104 70-71 0.014583916285786813 1104 72-73 -0.06738009091597519 1104 74-75 0.06081231245192242 1104 76-77 -0.011406893853919797 1104 78-79 0.19503192506531164 1104 80-81 0.10000541929210627 1104 82-83 0.24852343274205424 1104 84-85 0.08096821355004735 1104 86-87 0.0048419316530399215 1104 88-89 0.004742373887303586 1104 90-91 0.0694810007254496 1104 92-93 0.36646022716005433 1104 94-95 0.4448137230264493 1104 96-97 0.5698568155387456 1104 98-99 0.6248852858926845 1104 100-101 0.45789503671066 1105 1 0.1668517739520894 1105 2 0.14236455176411766 1105 3 0.10909993205211066 1105 4 0.09017602851110951 1105 5 0.09824307129848364 1105 6 0.09773152961860632 1105 7 0.10525109579114655 1105 8 0.10128120322885081 1105 9 0.1014790004573598 1105 10-11 0.15029559012703686 1105 12-13 0.15644663805924353 1105 14-15 0.2334280901034944 1105 16-17 0.12791350570331161 1105 18-19 0.13068470788569897 1105 20-21 0.14028267624116353 1105 22-23 0.15853539278326423 1105 24-25 0.20524703734441374 1105 26-27 0.310733910788791 1105 28-29 0.1775008119029522 1105 30-31 0.333931657026028 1105 32-33 0.20928119280814172 1105 34-35 0.38612665583372063 1105 36-37 0.34773342733349466 1105 38-39 0.34296591211827376 1105 40-41 0.17221184037821047 1105 42-43 0.20708130088323884 1105 44-45 0.2597157351046704 1105 46-47 0.272112384012658 1105 48-49 0.27292491186944545 1105 50-51 0.2686821072323653 1105 52-53 0.30932875499379975 1105 54-55 0.48993323115409027 1105 56-57 0.3908992039660646 1105 58-59 0.3328796816499775 1105 60-61 0.38987671372878907 1105 62-63 0.5433569827740641 1105 64-65 0.5251223516200483 1105 66-67 0.641460956502435 1105 68-69 0.6958843528766323 1105 70-71 0.7180297179144048 1105 72-73 0.7084675929713597 1105 74-75 0.6427856804935672 1105 76-77 0.6536495071606332 1105 78-79 0.7233208718013948 1105 80-81 0.7063547051017487 1105 82-83 0.7605945847911784 1105 84-85 0.8590569199977871 1105 86-87 0.8270936336954904 1105 88-89 0.6170755069803491 1105 90-91 0.7329321420950947 1105 92-93 0.9673596397885404 1105 94-95 0.8820183686011411 1105 96-97 1.0953690969430951 1105 98-99 1.1771196337768117 1105 100-101 1.0624972260044778 1106 1 -0.2162678214141991 1106 2 -0.09008446112542146 1106 3 -0.1231532903332706 1106 4 -0.05738801129962923 1106 5 -0.15700900897562775 1106 6 -0.13571275166708574 1106 7 -0.17148899231457904 1106 8 -0.27844795324038785 1106 9 -0.1786645792456909 1106 10-11 -0.19250421408246154 1106 12-13 -0.28854112503425 1106 14-15 -0.44024135026197797 1106 16-17 -0.4179520514838302 1106 18-19 -0.24301573264293808 1106 20-21 -0.21521087898807423 1106 22-23 -0.12467883466003826 1106 24-25 -0.10774529419417433 1106 26-27 -0.09914045697104257 1106 28-29 -0.15887869535761467 1106 30-31 -0.12049320836800348 1106 32-33 -0.2651355146482075 1106 34-35 -0.17736820466554093 1106 36-37 -0.2430872611964432 1106 38-39 -0.22190763984778528 1106 40-41 -0.18359008489508 1106 42-43 -0.2504305281263868 1106 44-45 -0.12497834223258053 1106 46-47 -0.07038394491538469 1106 48-49 -0.06955510118325492 1106 50-51 -0.07734987188331388 1106 52-53 -0.04819286354103269 1106 54-55 -0.47980571484036716 1106 56-57 -0.3967741522094883 1106 58-59 -0.22387019598095748 1106 60-61 -0.2832591975128551 1106 62-63 -0.16324115721615584 1106 64-65 -0.2129931647774086 1106 66-67 -0.15043168503778048 1106 68-69 -0.5034026433707197 1106 70-71 -0.4199699557680887 1106 72-73 -0.33594911448499687 1106 74-75 -0.23175502761542077 1106 76-77 -0.28981598476300974 1106 78-79 -0.541407468874084 1106 80-81 -0.4714508096641232 1106 82-83 -0.7056348164161967 1106 84-85 -0.5865149514331165 1106 86-87 -0.4246782703671599 1106 88-89 -0.2776397809948534 1106 90-91 -0.45796849422404406 1106 92-93 -0.3828916246917977 1106 94-95 -0.5496980614853335 1106 96-97 -0.375303117121188 1106 98-99 -0.3194834009760008 1106 100-101 -0.4732329094172876 1107 1 0.156656182533645 1107 2 0.11966672063137196 1107 3 0.10294509416623043 1107 4 0.07460409300664139 1107 5 0.0644527332715299 1107 6 0.06930620838694068 1107 7 0.12268585574526014 1107 8 0.10832883757566947 1107 9 0.09378879979955457 1107 10-11 0.045684970894946275 1107 12-13 0.2050057037019215 1107 14-15 0.2451765501068266 1107 16-17 0.18908602446027345 1107 18-19 0.20395013925982397 1107 20-21 0.08936187047948607 1107 22-23 0.20515234294112616 1107 24-25 0.2261994359497166 1107 26-27 0.1382903258454391 1107 28-29 0.2309831300021088 1107 30-31 0.3238982518158906 1107 32-33 0.23438658809970292 1107 34-35 0.25370238906984355 1107 36-37 0.2868935190227617 1107 38-39 0.3445481286837193 1107 40-41 0.43303970057061036 1107 42-43 0.42266229822029544 1107 44-45 0.48374380207161494 1107 46-47 0.4862125955240728 1107 48-49 0.46122979310794676 1107 50-51 0.6057065927159258 1107 52-53 0.7081055105694034 1107 54-55 0.6987600739498276 1107 56-57 0.6838502309394343 1107 58-59 0.7565116023671834 1107 60-61 0.6612034278053116 1107 62-63 0.5133242160112133 1107 64-65 0.47820677288816427 1107 66-67 0.4302363322080751 1107 68-69 0.42159709690251645 1107 70-71 0.42567182907426115 1107 72-73 0.4231475558178488 1107 74-75 0.4457495176030122 1107 76-77 0.47397550658808996 1107 78-79 0.5958389079375159 1107 80-81 0.6924695061636221 1107 82-83 0.4698173678859163 1107 84-85 0.6249177014160843 1107 86-87 0.6442449024550285 1107 88-89 0.7242167459388149 1107 90-91 0.6792747658663423 1107 92-93 0.8350378335181681 1107 94-95 0.7538217556670226 1107 96-97 0.9072016878240099 1107 98-99 0.862399098634409 1107 100-101 0.8681940294430319 1108 1 -0.03094819573870211 1108 2 0.05088561927526314 1108 3 0.09808567104803956 1108 4 0.05204391172659939 1108 5 -0.007961954804017068 1108 6 0.003926104270703945 1108 7 0.07664861148138868 1108 8 -0.06815666551138122 1108 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-0.5315347199303275 2316 52-53 -0.5766087957280988 2316 54-55 -0.6806360380113929 2316 56-57 -0.48430726399487156 2316 58-59 -0.39069146458376025 2316 60-61 -0.2897346586697225 2316 62-63 -0.4946947043914136 2316 64-65 -0.496970913657627 2316 66-67 -0.4389632074574692 2316 68-69 -0.6458695315853262 2316 70-71 -0.7891916482017436 2316 72-73 -0.5827434120335226 2316 74-75 -0.2940981379559311 2316 76-77 -0.3250348837660404 2316 78-79 -0.28548635920784804 2316 80-81 -0.5059019968721366 2316 82-83 -0.5529020706665477 2316 84-85 -0.43006983271231647 2316 86-87 -0.5233070812326446 2316 88-89 -0.5789392078599462 2316 90-91 -0.49572026419948045 2316 92-93 -0.5368828590991583 2316 94-95 -0.4758151929210612 2316 96-97 -0.5658945538114537 2316 98-99 -0.5877871143122242 2316 100-101 -0.3521386552151 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 7.0 5 15.0 6 32.0 7 109.0 8 242.0 9 521.0 10 892.0 11 1472.0 12 2202.0 13 3230.0 14 4445.0 15 5988.0 16 7559.0 17 10249.0 18 10163.0 19 7501.0 20 6734.0 21 6829.0 22 7247.0 23 7716.0 24 8811.0 25 10345.0 26 12255.0 27 15156.0 28 18465.0 29 22690.0 30 27351.0 31 34228.0 32 42380.0 33 55255.0 34 76972.0 35 114949.0 36 189758.0 37 312806.0 38 278298.0 39 35192.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50833322746001 18.63054218852876 14.548415375198886 23.31270920881235 2 16.168720068826286 19.553600859880063 39.719896162605835 24.557782908687823 3 19.917697195635828 23.081108471987 29.541564709944645 27.459629622432523 4 13.375089774355287 16.44598928163327 36.011006840413515 34.16791410359792 5 14.014963264445917 36.77269693747071 34.260434937600756 14.951904860482617 6 33.318635624108325 35.51768694925628 16.719516141447766 14.444161285187624 7 29.349955196898236 32.05136924108254 19.926805408059476 18.67187015395975 8 26.654400224801726 35.217866356567825 18.97459620886233 19.153137209768115 9 25.874934887610596 16.852992105800066 19.95932951241003 37.31274349417931 10-11 25.386842461233545 26.262203146754633 26.72200478746739 21.62894960454443 12-13 26.14442218198205 23.385986008279044 27.381794919126477 23.087796890612424 14-15 22.832393053613504 25.09889191728129 25.55110804535165 26.51760698375355 16-17 22.455181000488388 28.165168433942306 26.556478646773936 22.823171918795378 18-19 22.403755538732074 26.68082629026581 28.822780751780353 22.092637419221763 20-21 22.774810418234527 25.813200585616524 29.31355968303767 22.09842931311128 22-23 22.588460903221563 25.729012605403128 29.155235461038494 22.52729103033682 24-25 22.55041251529322 25.941154444918613 29.21164325255921 22.296789787228953 26-27 22.160299451889422 26.259187346176777 29.02395951494176 22.556553686992046 28-29 22.232502314889203 26.597198815912687 28.965784480265338 22.204514388932775 30-31 22.358092994648292 26.17700625599274 28.908575515459873 22.556325233899095 32-33 22.077594508215743 26.194863278980236 28.926481133482028 22.801061079321993 34-35 22.68942739262888 26.340155845243107 28.76618840687501 22.204228355253004 36-37 22.78808483542173 25.94030310322819 28.7501513423506 22.52146071899948 38-39 22.283216632936607 26.29673855560601 28.750380747891636 22.669664063565744 40-41 22.737621668004625 26.095815562442166 28.787274023778238 22.379288745774975 42-43 22.436366000878945 26.150329904242565 28.6267791958528 22.786524899025686 44-45 22.505301587954044 26.361494496624733 28.705777282859103 22.42742663256212 46-47 22.81808999709658 26.520344688812635 28.105866891765903 22.555698422324884 48-49 22.655983175856097 26.568874586440096 28.05346777502341 22.7216744626804 50-51 22.70837179026319 26.892921707794095 27.967374074885075 22.431332427057633 52-53 22.994566082519917 26.574928049731067 27.897310842350624 22.53319502539839 54-55 22.6658854460543 26.796963104514916 27.99431572323566 22.54283572619513 56-57 22.670406859993857 26.954838921825875 27.776353677703153 22.59840054047712 58-59 22.659420945132457 26.642823992939118 27.81966209717378 22.878092964754646 60-61 22.41040687494068 26.828762470321216 27.98822489288733 22.772605761850777 62-63 22.493810146949688 26.858730850317286 28.09001275711338 22.557446245619648 64-65 22.289487077350557 26.92367479599455 27.945439612219623 22.84139851443527 66-67 22.32065120318188 26.87461184969176 27.836552007096753 22.96818494002961 68-69 22.626036611591218 26.662660907640724 27.920850386768738 22.79045209399932 70-71 22.412387328980323 26.80200765725739 27.678115683925515 23.107489329836774 72-73 22.503301379517023 26.852938956427767 27.595235550719426 23.04852411333578 74-75 22.400840908258644 27.163832873593975 27.460751511310633 22.97457470683675 76-77 22.499452572609798 26.853462095230697 27.636488782035713 23.010596550123797 78-79 22.46077766825078 27.11473256023383 27.62991217994181 22.794577591573578 80-81 22.647127183263745 27.018699222839942 27.37080900426508 22.963364589631237 82-83 22.567199047588442 27.101990393676896 27.4862358444245 22.844574714310163 84-85 22.55547334514174 27.214524338019324 27.355178882386795 22.874823434452143 86-87 22.626386277853072 27.193634416123814 27.18231214274584 22.99766716327727 88-89 22.557633080906406 27.342746344193948 27.00506024690657 23.094560327993083 90-91 22.476617528912804 27.274592231377166 27.148590325654393 23.10019991405564 92-93 22.591621461750794 27.05109248482987 27.23639620693482 23.120889846484516 94-95 22.448446539324717 26.96903840361952 27.32294180379754 23.25957325325822 96-97 22.60420932742236 26.694335747745267 27.351734879035273 23.349720045797103 98-99 22.363884888537804 26.959920841625706 27.084502610836296 23.59169165900019 100-101 22.533120291283684 26.706161155150266 27.2661812436803 23.494537309885754 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 572.0 1 386.5 2 327.5 3 442.5 4 339.0 5 282.0 6 560.5 7 1308.5 8 2059.5 9 2304.0 10 1843.5 11 1265.5 12 1176.0 13 1223.0 14 1294.0 15 1400.0 16 1485.0 17 1580.0 18 1715.0 19 1922.0 20 2171.0 21 2476.0 22 3072.5 23 3871.0 24 4671.5 25 5845.0 26 7424.0 27 9305.5 28 11040.0 29 12690.5 30 14682.5 31 17202.5 32 20817.0 33 22551.0 34 23046.5 35 24835.0 36 27277.0 37 29971.0 38 32134.5 39 33798.5 40 35629.0 41 38081.5 42 39786.5 43 41348.5 44 43696.0 45 46495.5 46 47953.5 47 49211.0 48 50707.0 49 50641.0 50 50240.0 51 49710.5 52 49191.0 53 49840.5 54 50450.0 55 47439.5 56 43477.0 57 39739.0 58 36252.0 59 32322.5 60 26325.0 61 21728.0 62 18062.5 63 13263.0 64 9211.5 65 6783.5 66 4884.0 67 3665.0 68 2819.0 69 2169.5 70 1613.5 71 1034.5 72 713.0 73 533.0 74 369.5 75 230.5 76 154.0 77 94.5 78 55.0 79 32.0 80 17.0 81 9.0 82 4.0 83 2.0 84 4.5 85 4.0 86 1.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.016142568775937408 3 0.006800804438010667 4 0.0 5 0.0 6 0.0 7 0.0 8 6.726070323307253E-4 9 4.4840468822048354E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.7367057351706964E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004932451570425319 26-27 0.02238286735367247 28-29 0.0 30-31 0.0 32-33 4.110376308687766E-4 34-35 0.020589248600790536 36-37 0.0038114398498741106 38-39 0.018646161618501775 40-41 0.016067834661233994 42-43 0.008444954961485774 44-45 0.002578326957267781 46-47 3.7367057351706964E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.6156940146194877E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.003512503391060455 86-87 4.8577174557219054E-4 88-89 0.0 90-91 1.4946822940682786E-4 92-93 2.6156940146194877E-4 94-95 0.0 96-97 7.473411470341393E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1338077.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.9665330372225 #Duplication Level Percentage of deduplicated Percentage of total 1 83.71003428967724 56.057707768067 2 10.159324464156105 13.606694747295453 3 2.6441454943443388 5.312077698066996 4 1.150718017523244 3.0823838454799004 5 0.6086641471931421 2.038006386079121 6 0.3769594622777085 1.514620097058828 7 0.24894965724231438 1.1669906812425868 8 0.1729751127856239 0.9266834883980619 9 0.13588261468806603 0.8189628845123297 >10 0.7295864972714105 8.688462540391988 >50 0.03847244929868238 1.739058031937804 >100 0.021279713891190894 2.7387226920688708 >500 0.002116796791392111 0.9229712854452864 >1k 7.798725020918304E-4 1.0042190204586172 >5k 1.1141035744169006E-4 0.382438833497093 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5126 0.38308707196969977 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4682 0.34990512504138405 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2964 0.22151191598091888 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 7.473411470341393E-5 7 0.0 0.0 0.0 0.0 7.473411470341393E-5 8 0.0 0.0 0.0 0.0 7.473411470341393E-5 9 0.0 0.0 0.0 7.473411470341393E-5 7.473411470341393E-5 10-11 0.0 0.0 0.0 7.473411470341393E-5 7.473411470341393E-5 12-13 0.0 0.0 0.0 7.473411470341393E-5 2.6156940146194877E-4 14-15 0.0 0.0 0.0 7.473411470341393E-5 3.3630351616536266E-4 16-17 0.0 0.0 0.0 1.1210117205512089E-4 4.1103763086877665E-4 18-19 0.0 0.0 0.0 1.4946822940682786E-4 4.484046882204836E-4 20-21 0.0 0.0 0.0 2.242023441102418E-4 5.978729176273114E-4 22-23 0.0 0.0 0.0 4.8577174557219054E-4 5.978729176273114E-4 24-25 0.0 0.0 0.0 9.715434911443811E-4 5.978729176273114E-4 26-27 0.0 0.0 0.0 0.0013825811220131577 5.978729176273114E-4 28-29 0.0 0.0 0.0 0.004259844538094594 5.978729176273114E-4 30-31 0.0 0.0 0.0 0.012368495983415006 5.978729176273114E-4 32-33 0.0 0.0 0.0 0.028996836504924607 5.978729176273114E-4 34-35 0.0 0.0 0.0 0.05593848485550533 6.726070323307253E-4 36-37 0.0 0.0 0.0 0.10253520537308392 6.726070323307253E-4 38-39 0.0 0.0 0.0 0.16818912514003304 7.473411470341393E-4 40-41 0.0 0.0 0.0 0.2344782848819612 7.473411470341393E-4 42-43 0.0 0.0 0.0 0.3024489622047162 7.473411470341393E-4 44-45 0.0 0.0 0.0 0.38547856364020905 7.847082043858462E-4 46-47 0.0 0.0 0.0 0.48338025390168127 9.341764337926742E-4 48-49 0.0 0.0 0.0 0.5893532285511223 0.0011210117205512089 50-51 0.0 0.0 0.0 0.6976055936990173 0.0012704799499580369 52-53 0.0 0.0 0.0 0.8011123425632456 0.0014573152367165715 54-55 0.0 0.0 0.0 0.9113825288081329 0.0015694164087716924 56-57 0.0 0.0 0.0 1.0391031308362673 0.0016815175808268133 58-59 0.0 0.0 0.0 1.1771744077508246 0.0018309858102336413 60-61 0.0 0.0 0.0 1.322233324390151 0.0018683528675853482 62-63 0.0 0.0 0.0 1.4597067283870808 0.00209255521169559 64-65 0.0 0.0 0.0 1.6089133883924467 0.00209255521169559 66-67 0.0 0.0 0.0 1.7737021113134745 0.00209255521169559 68-69 0.0 0.0 0.0 1.9569501605662456 0.00209255521169559 70-71 0.0 0.0 0.0 2.1465879766261584 0.002279390498454125 72-73 7.473411470341393E-5 0.0 0.0 2.3404482701668137 0.0025035928425643666 74-75 7.473411470341393E-5 0.0 0.0 2.5360274483456484 0.0026904281293229013 76-77 1.4946822940682786E-4 0.0 0.0 2.743975122507898 0.0027651622440263155 78-79 1.4946822940682786E-4 0.0 0.0 2.97213837469742 0.0028772634160814364 80-81 1.4946822940682786E-4 0.0 0.0 3.2148000451394054 0.003026731645488264 82-83 1.4946822940682786E-4 0.0 0.0 3.4627678377253326 0.003138832817543385 84-85 1.4946822940682786E-4 0.0 0.0 3.7080825692392887 0.003138832817543385 86-87 1.4946822940682786E-4 0.0 0.0 3.9576197782339877 0.00332566810430192 88-89 1.8683528675853483E-4 0.0 0.0 4.243926171662767 0.003437769276357041 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2440 0.0 51.5981 1 GTATCAA 4265 0.0 39.990158 1 ATCAACG 5355 0.0 31.224531 3 TCAACGC 5415 0.0 30.787378 4 TATCAAC 5520 0.0 30.467854 2 CAACGCA 5510 0.0 30.256563 5 AACGCAG 5680 0.0 29.350994 6 GGACCGA 355 0.0 25.420805 6 ACGCAGA 6655 0.0 24.836779 7 AACCGAC 115 1.2824996E-4 24.780922 7 CGCAGAG 6705 0.0 24.651567 8 TGGTATC 1205 0.0 24.444567 2 GTGGTAT 1420 0.0 22.41631 1 GCAGAGT 7355 0.0 22.279245 9 GAGTACT 4765 0.0 22.028933 12-13 AGGACCG 470 0.0 21.22196 5 GTACTTT 4970 0.0 20.92916 14-15 AGAGTAC 6570 0.0 20.856647 10-11 GTACCGG 115 0.0037194197 20.656172 1 GTACCTG 1090 0.0 20.485617 1 >>END_MODULE