FastQCFastQC Report
Thu 26 May 2016
SRR938060_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938060_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290238
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48860.3786898231179054No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42850.33210926976263294No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25460.19732793484612915No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25100.049.715581
GTATCAA42050.040.6624341
ATCAACG52350.032.53053
TATCAAC52850.032.2214852
TCAACGC53050.032.1012544
CAACGCA53650.031.7422495
AACGCAG55100.030.9045226
ACGCAGA62500.027.08697
CGCAGAG62900.027.0595428
GTACATG36200.025.129841
TGGTATC12050.024.8691542
TACATGG36150.024.6059862
GCAGAGT68200.023.9110039
GAGTACT46250.023.23280512-13
GTGGTAT14300.022.981481
ACATGGG36900.022.6887553
GTACTTT48400.021.85440414-15
CAGAGTA66300.021.0812610-11
TAAGGTG6650.020.7443925
GTATAAG4600.020.707951
Produced by FastQC (version 0.11.5)