##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938060_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1290238 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.68601219309926 33.0 31.0 34.0 30.0 34.0 2 31.811754110481942 33.0 31.0 34.0 30.0 34.0 3 31.833162563806056 34.0 31.0 34.0 30.0 34.0 4 35.36019866102223 37.0 35.0 37.0 33.0 37.0 5 35.35274112218056 37.0 35.0 37.0 33.0 37.0 6 35.368157657734464 37.0 35.0 37.0 33.0 37.0 7 35.28393443690234 37.0 35.0 37.0 33.0 37.0 8 35.26382651882831 37.0 35.0 37.0 33.0 37.0 9 36.85393160021639 39.0 37.0 39.0 33.0 39.0 10-11 36.74735320150236 39.0 37.0 39.0 32.5 39.0 12-13 36.574588951805794 39.0 37.0 39.0 32.0 39.0 14-15 37.682300862321526 40.0 38.0 41.0 32.0 41.0 16-17 37.473894738800126 40.0 37.0 41.0 32.0 41.0 18-19 37.647861867345405 40.0 38.0 41.0 32.0 41.0 20-21 37.63146217984588 40.0 38.0 41.0 32.0 41.0 22-23 37.49689863420547 40.0 37.5 41.0 32.0 41.0 24-25 37.36014828271993 40.0 37.0 41.0 31.5 41.0 26-27 37.14712595660646 40.0 37.0 41.0 31.0 41.0 28-29 36.938266040838975 40.0 37.0 41.0 30.0 41.0 30-31 36.69521049604802 39.5 36.0 41.0 30.0 41.0 32-33 36.37902890784491 39.0 35.5 41.0 30.0 41.0 34-35 36.10779251579942 39.0 35.0 41.0 29.0 41.0 36-37 35.807887382017896 39.0 35.0 40.0 27.5 41.0 38-39 35.47502553792401 38.0 35.0 40.0 26.5 41.0 40-41 35.33187559194505 38.0 35.0 40.0 26.0 41.0 42-43 35.08794927757515 38.0 34.5 40.0 25.0 41.0 44-45 34.733546058944164 38.0 34.0 40.0 24.0 41.0 46-47 34.33258282580423 38.0 33.5 40.0 23.0 41.0 48-49 33.89320574963689 38.0 33.0 40.0 20.0 41.0 50-51 33.16778106054852 37.5 32.5 39.5 18.5 40.5 52-53 33.246101106927554 37.5 33.0 39.5 18.5 40.5 54-55 33.290663040462306 38.0 33.0 40.0 16.5 41.0 56-57 32.929883091336634 37.5 32.0 40.0 13.0 41.0 58-59 32.46881505582691 37.0 31.5 40.0 8.5 41.0 60-61 31.913591910949762 36.0 31.0 39.0 6.5 41.0 62-63 31.310119915860483 35.5 30.0 39.0 2.0 40.5 64-65 30.706544451488796 35.0 29.5 39.0 2.0 40.0 66-67 30.061055402181616 35.0 28.5 38.0 2.0 40.0 68-69 29.30985872373934 34.0 27.5 37.5 2.0 40.0 70-71 28.66423752826998 34.0 26.0 37.0 2.0 39.0 72-73 27.90988213027364 34.0 26.0 36.0 2.0 39.0 74-75 27.176639116194067 33.0 24.5 36.0 2.0 38.5 76-77 26.36618205323359 32.5 21.5 35.0 2.0 37.5 78-79 25.562989153939043 32.0 19.0 35.0 2.0 37.0 80-81 24.792847908680415 31.0 15.0 35.0 2.0 36.0 82-83 24.00475842441472 31.0 7.5 34.0 2.0 36.0 84-85 23.225853292183302 30.0 2.0 34.0 2.0 35.0 86-87 22.42518783356249 29.5 2.0 34.0 2.0 35.0 88-89 21.498498339066124 29.0 2.0 34.0 2.0 35.0 90-91 20.558215616033632 28.0 2.0 34.0 2.0 35.0 92-93 19.58341678046996 26.5 2.0 33.5 2.0 35.0 94-95 18.480542349551015 24.5 2.0 33.0 2.0 35.0 96-97 17.205989127587316 20.5 2.0 32.0 2.0 34.0 98-99 15.663567884374821 2.0 2.0 32.0 2.0 34.0 100-101 12.102109455774826 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3758660063076604 1101 2 0.3335522674232152 1101 3 0.1948436191781191 1101 4 0.06683352859440816 1101 5 0.1768943088217867 1101 6 0.29246399892132047 1101 7 0.2758934576219474 1101 8 0.4377417752573365 1101 9 0.37044543282917886 1101 10-11 0.35223503191188854 1101 12-13 0.346495116278696 1101 14-15 0.46338670409163996 1101 16-17 0.4968006874379185 1101 18-19 0.545662218200853 1101 20-21 0.5504625119010029 1101 22-23 0.5603781506184404 1101 24-25 0.49381298476994573 1101 26-27 0.7005870645513212 1101 28-29 0.5445617322019629 1101 30-31 0.4860786758347828 1101 32-33 0.43507641520367457 1101 34-35 0.5092607889141831 1101 36-37 0.5075104342354138 1101 38-39 0.3608416041975815 1101 40-41 0.41079813127136333 1101 42-43 0.4107667833482864 1101 44-45 0.6943074028849168 1101 46-47 0.9300510425851201 1101 48-49 0.982249363727945 1101 50-51 0.8342598461548434 1101 52-53 0.768651692778981 1101 54-55 0.6288645495339651 1101 56-57 0.4916974192118815 1101 58-59 0.580222243311411 1101 60-61 0.5585371657338385 1101 62-63 0.6871717414528966 1101 64-65 0.4159230387026085 1101 66-67 0.5563923073031738 1101 68-69 0.3090488503746798 1101 70-71 0.22181532255860859 1101 72-73 -0.025302120745095635 1101 74-75 0.13915260103619786 1101 76-77 -0.23794221083290168 1101 78-79 -0.5457477560658397 1101 80-81 -0.4838114586861302 1101 82-83 -0.5869643928279871 1101 84-85 -0.5444304646201097 1101 86-87 -0.3983989698158119 1101 88-89 -0.3376518752859745 1101 90-91 -0.43419008527079583 1101 92-93 -0.6972378585329224 1101 94-95 -1.0642106956514006 1101 96-97 -1.0439352575951375 1101 98-99 -1.057986160217082 1101 100-101 -0.8963111259878218 1102 1 0.45472446621632656 1102 2 0.41714781807959156 1102 3 0.45826251314115396 1102 4 0.3727001341804268 1102 5 0.3588578608524813 1102 6 0.4464530902989239 1102 7 0.4311652426562347 1102 8 0.6323347061264499 1102 9 0.46299120752747314 1102 10-11 0.5270278110337614 1102 12-13 0.5566378445795621 1102 14-15 0.7216290129402196 1102 16-17 0.6541554180865745 1102 18-19 0.7809599147922626 1102 20-21 0.6102387562999496 1102 22-23 0.8504780747448208 1102 24-25 0.7280520296212742 1102 26-27 0.7395166738737586 1102 28-29 0.6986088024781125 1102 30-31 0.7862139599313878 1102 32-33 0.7992139611931108 1102 34-35 0.7732401455631432 1102 36-37 0.6702729498038238 1102 38-39 0.6225690890586506 1102 40-41 1.016936736628324 1102 42-43 1.0769321592090222 1102 44-45 1.0430390250056547 1102 46-47 0.9674273900576651 1102 48-49 0.9278866305597191 1102 50-51 0.9097662667810198 1102 52-53 0.8615074040583863 1102 54-55 0.7600121022518209 1102 56-57 0.6829061002992987 1102 58-59 0.7524746877304054 1102 60-61 0.7368680892446378 1102 62-63 0.6587019549011437 1102 64-65 0.6816367517857991 1102 66-67 0.3677611819399118 1102 68-69 0.4722816392312801 1102 70-71 0.337177583306179 1102 72-73 0.43003909580967203 1102 74-75 0.5416306621073055 1102 76-77 0.18268181390476101 1102 78-79 0.13164405244631894 1102 80-81 0.364170732486766 1102 82-83 0.6116126188966362 1102 84-85 0.7671660308775792 1102 86-87 0.6688264893627469 1102 88-89 0.6893992177236328 1102 90-91 0.6342529307771905 1102 92-93 0.5255922275049016 1102 94-95 0.7115226300468542 1102 96-97 0.5227948756847489 1102 98-99 0.10295682004146123 1102 100-101 -0.028310467462059563 1103 1 0.3979586210121795 1103 2 0.4767108709095993 1103 3 0.34732640492630296 1103 4 0.36686404460901656 1103 5 0.4022473777109923 1103 6 0.3960805665975826 1103 7 0.38288078507698486 1103 8 0.39194885581261474 1103 9 0.34957275880040584 1103 10-11 0.45711108509934206 1103 12-13 0.4996382657833678 1103 14-15 0.5564566991502886 1103 16-17 0.5918521237203507 1103 18-19 0.541640193477285 1103 20-21 0.639988519804362 1103 22-23 0.6421834668441448 1103 24-25 0.38879100064107064 1103 26-27 0.4577012854340765 1103 28-29 0.6107125429060858 1103 30-31 0.684128310552488 1103 32-33 0.6275537276666654 1103 34-35 0.5714179211927117 1103 36-37 0.5538363340728978 1103 38-39 0.2977815898354592 1103 40-41 0.4595919088040148 1103 42-43 0.5647921676331009 1103 44-45 0.5125642385421685 1103 46-47 0.6938168447713551 1103 48-49 0.43789125689669817 1103 50-51 0.3850077684761928 1103 52-53 0.5207535182805714 1103 54-55 0.3893575791442956 1103 56-57 0.30430774230368485 1103 58-59 0.4523800894966925 1103 60-61 0.38288460595813945 1103 62-63 0.3885994849895944 1103 64-65 0.3452354205055457 1103 66-67 0.3123621776381107 1103 68-69 0.2709234295546388 1103 70-71 0.07304205234769867 1103 72-73 0.11943442666181525 1103 74-75 0.12003809739037408 1103 76-77 0.24032562738742413 1103 78-79 -0.11293968080047279 1103 80-81 0.045063270274269485 1103 82-83 -0.2756873956038248 1103 84-85 -0.34553499036904967 1103 86-87 -0.5809725757028836 1103 88-89 -0.2799174653999046 1103 90-91 -0.27207375766318265 1103 92-93 -0.3917368569637283 1103 94-95 -0.29483547605508065 1103 96-97 -0.3686281027095646 1103 98-99 -0.5062219706202438 1103 100-101 -0.9064234053241407 1104 1 0.4011017572902347 1104 2 0.39880599316065 1104 3 0.4151671647655881 1104 4 0.2691072009441413 1104 5 0.22667364858489947 1104 6 0.3440247173749498 1104 7 0.3952954616729727 1104 8 0.49002014250572756 1104 9 0.5545216779833666 1104 10-11 0.6219361680773687 1104 12-13 0.5751715572772369 1104 14-15 0.6746859837600496 1104 16-17 0.4712201967539471 1104 18-19 0.6319028116360457 1104 20-21 0.5944013977344724 1104 22-23 0.5897588264155615 1104 24-25 0.7264639928317607 1104 26-27 0.7564590413719401 1104 28-29 0.632275182415917 1104 30-31 0.6297526352440386 1104 32-33 0.655503248561601 1104 34-35 0.7858848884996945 1104 36-37 0.6052461516829979 1104 38-39 0.2251992749912901 1104 40-41 0.4669632039081506 1104 42-43 0.5776335421243957 1104 44-45 0.6438694359101689 1104 46-47 0.2756157047536263 1104 48-49 0.20457226544359486 1104 50-51 0.3729337384941118 1104 52-53 0.49738380186160214 1104 54-55 0.565788586073694 1104 56-57 0.6694375632781941 1104 58-59 0.42461787571425447 1104 60-61 0.5374999955504691 1104 62-63 0.5802628627288371 1104 64-65 0.31489619729628515 1104 66-67 0.3974740119538076 1104 68-69 0.4180898837889444 1104 70-71 0.4711748042417945 1104 72-73 0.35118314398012274 1104 74-75 0.17276941682179014 1104 76-77 -0.1278984812054098 1104 78-79 0.21494319887654356 1104 80-81 0.2627183405063498 1104 82-83 0.0762953423797299 1104 84-85 0.1558049510125592 1104 86-87 0.14056935973924567 1104 88-89 0.29553300982314923 1104 90-91 -0.009242735205738484 1104 92-93 -0.45510650868248703 1104 94-95 -0.4683556204659496 1104 96-97 -0.6577516736436415 1104 98-99 -0.41378704285803103 1104 100-101 -0.582118447346927 1105 1 0.3153310506222411 1105 2 0.25587641078249135 1105 3 0.3420036045144137 1105 4 0.35363700259868125 1105 5 0.33780690882610287 1105 6 0.2976178600042729 1105 7 0.40097297587610825 1105 8 0.37024290738163756 1105 9 0.3445184386730915 1105 10-11 0.4228593715060498 1105 12-13 0.3979333184653129 1105 14-15 0.5071837411606239 1105 16-17 0.43621384272224617 1105 18-19 0.6104062502884844 1105 20-21 0.43144727135154426 1105 22-23 0.43442393156855985 1105 24-25 0.2946932114104541 1105 26-27 0.5267520743630669 1105 28-29 0.48480309031983637 1105 30-31 0.16939992419050753 1105 32-33 0.22350260069953976 1105 34-35 0.13244062955421754 1105 36-37 0.06115290938258511 1105 38-39 0.12903848061274203 1105 40-41 0.18262589203732205 1105 42-43 0.4129083851424369 1105 44-45 0.28114268419084 1105 46-47 0.07898334155218834 1105 48-49 0.21987551472098232 1105 50-51 0.06832519668208903 1105 52-53 0.022105345766711082 1105 54-55 -0.034757736063255606 1105 56-57 -0.03003667174622393 1105 58-59 -0.08002119036767397 1105 60-61 -0.0906795120743702 1105 62-63 -0.075442683967065 1105 64-65 0.056149436606563796 1105 66-67 -0.034360932728741744 1105 68-69 0.05945587601459934 1105 70-71 0.07284865553253539 1105 72-73 -0.2376219242945723 1105 74-75 -0.11799730300279165 1105 76-77 -0.3472265735008335 1105 78-79 -0.49621933974600196 1105 80-81 -0.1338859902023124 1105 82-83 -0.4455681331510206 1105 84-85 -0.2978430107137413 1105 86-87 -0.14534088644833432 1105 88-89 -0.04170784535476102 1105 90-91 -0.10956355151192909 1105 92-93 -0.34853818415342985 1105 94-95 -0.5806743436793447 1105 96-97 -0.7276379240174116 1105 98-99 -0.9802570667790906 1105 100-101 -0.751736831678631 1106 1 0.3524578377801788 1106 2 0.2232154419795478 1106 3 0.366348267127794 1106 4 0.3350739550998725 1106 5 0.28922832924981634 1106 6 0.3089416027642784 1106 7 0.3816471677097226 1106 8 0.3153919710271609 1106 9 0.5213633227777734 1106 10-11 0.47329224856730434 1106 12-13 0.521409527530956 1106 14-15 0.5471269286419371 1106 16-17 -0.019374728880350744 1106 18-19 0.4282282709536318 1106 20-21 0.6720916864483826 1106 22-23 0.6644744869579995 1106 24-25 0.6435243602858307 1106 26-27 0.6817433808295092 1106 28-29 0.6179269759417281 1106 30-31 0.5696039038354641 1106 32-33 0.6249258207400814 1106 34-35 0.4931464808006538 1106 36-37 0.4018778227241171 1106 38-39 0.40389568793967356 1106 40-41 0.42050651099476966 1106 42-43 0.24626871272806738 1106 44-45 0.5320584122881229 1106 46-47 0.7635859584719142 1106 48-49 0.9061033240612701 1106 50-51 0.6498803701651568 1106 52-53 0.7856602673034132 1106 54-55 0.8216940978847163 1106 56-57 0.6049581232081991 1106 58-59 0.49049714080612006 1106 60-61 0.6395122440194143 1106 62-63 0.6121088819764289 1106 64-65 0.5549661885473789 1106 66-67 0.3575789988304514 1106 68-69 0.1439866466935804 1106 70-71 0.25507681649026637 1106 72-73 0.18543511248405764 1106 74-75 0.37819373878154394 1106 76-77 0.04616101135801998 1106 78-79 0.4268854560943929 1106 80-81 0.4509510614162373 1106 82-83 0.5791824814873436 1106 84-85 0.15912953543950792 1106 86-87 0.31983445160276247 1106 88-89 -0.04379332438507433 1106 90-91 -0.14905025926523408 1106 92-93 -0.2762351176063724 1106 94-95 -0.6204116134668247 1106 96-97 -1.105651936110835 1106 98-99 -1.0450643831904873 1106 100-101 -1.0587151227625 1107 1 -0.05002775864770115 1107 2 -0.08104809095356913 1107 3 -0.07091753900929376 1107 4 0.019810987160930438 1107 5 -0.15048263560527886 1107 6 -0.24856781223055435 1107 7 0.016007127574347635 1107 8 -0.11420200791121715 1107 9 -0.04640857830479206 1107 10-11 -0.010492985621837647 1107 12-13 -0.0013262913988043579 1107 14-15 -0.11628865952832967 1107 16-17 0.04531615884075535 1107 18-19 -0.022353635249778847 1107 20-21 0.10428955156406516 1107 22-23 0.1293380852717121 1107 24-25 0.09384266553006881 1107 26-27 -0.01896654662826336 1107 28-29 -0.0328121460308779 1107 30-31 -0.16105449388642512 1107 32-33 0.07850654215638286 1107 34-35 0.10121626041513565 1107 36-37 0.04379056419298877 1107 38-39 0.014390785339749357 1107 40-41 0.024484334501167382 1107 42-43 0.025053654027253458 1107 44-45 0.19005459149141757 1107 46-47 -0.19916788736215096 1107 48-49 -0.2639126809575387 1107 50-51 -0.34406909025596377 1107 52-53 -0.2406790428746035 1107 54-55 -0.5143319334186103 1107 56-57 -0.5610500134636993 1107 58-59 -0.48955421886463313 1107 60-61 -0.6350163448745825 1107 62-63 -0.5441545224630246 1107 64-65 -0.6525680748532814 1107 66-67 -0.8837972816182962 1107 68-69 -0.8853306929586147 1107 70-71 -0.8701827690421062 1107 72-73 -0.8016002553951722 1107 74-75 -1.077054565763543 1107 76-77 -1.1142850505948196 1107 78-79 -1.176678542250304 1107 80-81 -1.4012321025133296 1107 82-83 -1.478526287637898 1107 84-85 -1.8480453236426335 1107 86-87 -1.9084496478740043 1107 88-89 -1.8759428154179183 1107 90-91 -1.7013046615902105 1107 92-93 -1.7649314549702488 1107 94-95 -1.9564726026034442 1107 96-97 -1.71510585696252 1107 98-99 -1.8614526154389939 1107 100-101 -1.7847439695886127 1108 1 -0.09894909689237963 1108 2 -0.11217627493589788 1108 3 -0.11516819342109841 1108 4 0.09303102026090215 1108 5 0.12344804043034685 1108 6 0.1143700228632909 1108 7 0.0860316178791436 1108 8 -0.029925314986321894 1108 9 0.14699022942154016 1108 10-11 0.020208802379912072 1108 12-13 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-0.12003209884809252 2316 80-81 -0.40206436948946944 2316 82-83 -0.5593929588556463 2316 84-85 -0.44751357331110597 2316 86-87 -0.22495971375333212 2316 88-89 -0.20417336934751162 2316 90-91 -0.29176951586118705 2316 92-93 -0.17567505815852158 2316 94-95 -0.14632267350832606 2316 96-97 -0.16448112415701388 2316 98-99 -0.5030125616587657 2316 100-101 -0.5843278845163056 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5701.0 3 2030.0 4 1944.0 5 2165.0 6 2513.0 7 3020.0 8 3725.0 9 4584.0 10 5792.0 11 6850.0 12 8304.0 13 9811.0 14 11173.0 15 12930.0 16 14498.0 17 17624.0 18 17602.0 19 16519.0 20 17207.0 21 18316.0 22 20297.0 23 22115.0 24 24537.0 25 27351.0 26 30273.0 27 34252.0 28 37887.0 29 43785.0 30 49760.0 31 58494.0 32 69339.0 33 83807.0 34 103690.0 35 129545.0 36 152487.0 37 149311.0 38 67293.0 39 3706.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24794421138824 18.16886377659719 14.137383725217026 23.445808286797547 2 15.866615279749807 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28.533984425428415 22.550486322530173 46-47 22.886744953106845 26.564646410208592 27.921909489171092 22.626699147513474 48-49 22.70275467451047 26.62640087990785 27.867056995899368 22.803787449682307 50-51 22.75442438416884 26.92232532387066 27.775059361424614 22.54819093053589 52-53 22.985428811423446 26.68797825582202 27.70285644291824 22.623736489836293 54-55 22.659062764374408 26.807128716556516 27.82279170068882 22.71101681838026 56-57 22.644701139623226 26.95530661291573 27.689549577486627 22.71044266997442 58-59 22.69902233553866 26.631779988275472 27.671052509142342 22.998145167043525 60-61 22.54478944483341 26.763374806261954 27.882151444031706 22.809684304872935 62-63 22.55498293245408 26.72823442014544 28.09352177682381 22.623260870576665 64-65 22.373015005361548 26.79138957572036 27.93320974243626 22.90238567648183 66-67 22.336098859583863 26.85686395448415 27.7680105850094 23.039026600922583 68-69 22.6418459930092 26.602924285467218 27.84345386142023 22.91177586010335 70-71 22.522087985926966 26.66509506935901 27.568660126702277 23.244156818011746 72-73 22.550485351067245 26.658250502876125 27.614800764899012 23.176463381157618 74-75 22.48647638557683 26.99116879773682 27.490281567991033 23.032073248695326 76-77 22.675546497597416 26.703292997597995 27.462499937013767 23.15866056779082 78-79 22.69478200098686 26.79176726822815 27.50330730257836 23.010143428206632 80-81 22.840642246856174 26.721962557048595 27.274133291573282 23.16326190452195 82-83 22.725857947592683 26.83495955474364 27.271680723374914 23.167501774288763 84-85 22.707131611199785 26.838794749412592 27.252984327773337 23.201089311614282 86-87 22.89168815342085 26.763180049955736 27.15133703685902 23.19379475976439 88-89 22.876370021312304 26.77459058349801 26.990660743479893 23.358378651709792 90-91 22.77588603560631 26.814291095862508 26.946547086663173 23.46327578186801 92-93 22.895226437903666 26.461329147774055 27.13031276506377 23.513131649258508 94-95 22.734777157213347 26.34853830381268 27.150678481910056 23.76600605706392 96-97 22.982034130846934 26.047970255044845 27.148786434215694 23.821209179892527 98-99 22.783477573050437 26.232474098889508 26.825097710776102 24.15895061728395 100-101 23.73793464198376 25.063620340814108 26.917360689109763 24.281084328092366 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 491.0 1 352.5 2 334.0 3 457.5 4 367.5 5 298.5 6 532.0 7 1098.5 8 1746.0 9 2028.0 10 1685.0 11 1234.0 12 1125.0 13 1169.5 14 1277.0 15 1368.0 16 1462.0 17 1616.5 18 1707.5 19 1868.5 20 2212.5 21 2590.5 22 3092.0 23 3876.5 24 4823.0 25 5898.5 26 7384.5 27 9177.5 28 10683.5 29 12150.0 30 13915.5 31 16283.5 32 19533.5 33 21324.5 34 22045.0 35 23786.5 36 26094.0 37 28434.0 38 30594.5 39 32164.0 40 33781.0 41 36054.5 42 37715.5 43 39434.5 44 41606.5 45 43834.0 46 45360.5 47 46446.5 48 47350.0 49 47355.0 50 47382.0 51 47105.5 52 46725.0 53 47153.0 54 46992.5 55 44692.0 56 41782.5 57 38835.0 58 35837.0 59 31763.5 60 26475.5 61 22289.5 62 18675.0 63 14478.0 64 10629.5 65 8130.5 66 6168.5 67 4633.0 68 3611.0 69 2801.0 70 2157.0 71 1515.5 72 1021.5 73 728.5 74 512.0 75 371.0 76 262.5 77 181.5 78 127.0 79 80.0 80 45.5 81 29.5 82 19.0 83 12.5 84 6.5 85 7.0 86 7.5 87 4.0 88 5.0 89 3.5 90 1.5 91 2.0 92 1.0 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1397416600658173 2 0.05231592930916622 3 0.1512899170540629 4 0.12982100976719024 5 0.1942277316278082 6 0.1986455212139156 7 0.1658608721801714 8 0.0696770673317636 9 0.12168297631909772 10-11 0.10265547906665282 12-13 0.15214247293910116 14-15 0.13733900257161857 16-17 0.13524636539925192 18-19 0.1360989212842902 20-21 0.13695147716932843 22-23 0.20011811774261803 24-25 0.2180217913284216 26-27 0.15039860862879562 28-29 0.13055730803154147 30-31 0.10312050954940097 32-33 0.12815465053734273 34-35 0.09757889629665226 36-37 0.0909909644577202 38-39 0.050843332780463754 40-41 0.05816756288374703 42-43 0.0616165389641291 44-45 0.03933382833244719 46-47 0.0509595904011508 48-49 0.04320908235534839 50-51 0.05270345471145634 52-53 0.02553792401091892 54-55 0.049370736251761305 56-57 0.02619671719481212 58-59 0.04913822101038723 60-61 0.037279943700309556 62-63 0.050843332780463754 64-65 0.03867503514855399 66-67 0.03793873688420276 68-69 0.06425171169970191 70-71 0.052664702171227325 72-73 0.050843332780463754 74-75 0.0425890417116842 76-77 0.02119763950526957 78-79 0.022437720792597954 80-81 0.025499171470689905 82-83 0.022050195390307833 84-85 0.019298765034047982 86-87 0.022941503815575113 88-89 0.06525927774565622 90-91 0.11234361412390582 92-93 0.08087655145794807 94-95 0.07746632791779502 96-97 0.06793320302145806 98-99 0.055648647768861255 100-101 0.13447131459467168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1290238.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.75337266741893 #Duplication Level Percentage of deduplicated Percentage of total 1 85.48885466265207 59.63135938194786 2 9.071527592987739 12.65539289712895 3 2.3706379501442956 4.960799771878235 4 1.0208821555100074 2.848398937712299 5 0.5510652133562192 1.921932859564354 6 0.3281831203789347 1.3735127699368948 7 0.2192006456705126 1.0702989028475882 8 0.15056044158303372 0.8401678872570009 9 0.11260792427547496 0.7069304256562526 >10 0.6325776900834684 7.852105661132303 >50 0.03322924458149025 1.5691144721982568 >100 0.01811700077203652 2.4978154108538613 >500 0.0016671900030799827 0.7455875812136722 >1k 8.891680016426574E-4 1.3265830406724701 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4886 0.3786898231179054 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4285 0.33210926976263294 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2546 0.19732793484612915 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.750508045802403E-5 2 0.0 0.0 0.0 0.0 7.750508045802403E-5 3 0.0 0.0 0.0 0.0 7.750508045802403E-5 4 0.0 0.0 0.0 0.0 7.750508045802403E-5 5 0.0 0.0 0.0 0.0 7.750508045802403E-5 6 0.0 0.0 0.0 0.0 7.750508045802403E-5 7 0.0 0.0 0.0 0.0 7.750508045802403E-5 8 0.0 0.0 0.0 0.0 7.750508045802403E-5 9 0.0 0.0 0.0 0.0 1.5501016091604806E-4 10-11 0.0 0.0 0.0 0.0 1.5501016091604806E-4 12-13 0.0 0.0 0.0 7.750508045802403E-5 3.487728620611081E-4 14-15 0.0 0.0 0.0 1.5501016091604806E-4 5.425355632061681E-4 16-17 0.0 7.750508045802403E-5 0.0 1.5501016091604806E-4 6.200406436641922E-4 18-19 0.0 7.750508045802403E-5 0.0 2.7126778160308407E-4 6.200406436641922E-4 20-21 0.0 7.750508045802403E-5 0.0 3.100203218320961E-4 6.975457241222162E-4 22-23 0.0 7.750508045802403E-5 0.0 4.2627794251913216E-4 6.975457241222162E-4 24-25 0.0 7.750508045802403E-5 0.0 6.200406436641922E-4 7.750508045802402E-4 26-27 0.0 7.750508045802403E-5 0.0 0.0010075660459543123 7.750508045802402E-4 28-29 0.0 7.750508045802403E-5 0.0 0.0024801625746567685 7.750508045802402E-4 30-31 0.0 7.750508045802403E-5 0.0 0.009029341873359797 7.750508045802402E-4 32-33 0.0 7.750508045802403E-5 0.0 0.026080459574125085 7.750508045802402E-4 34-35 0.0 7.750508045802403E-5 0.0 0.05200590898733412 7.750508045802402E-4 36-37 0.0 7.750508045802403E-5 0.0 0.09955527584833186 7.750508045802402E-4 38-39 0.0 7.750508045802403E-5 0.0 0.16930984826055348 7.750508045802402E-4 40-41 0.0 7.750508045802403E-5 0.0 0.23371657012117145 7.750508045802402E-4 42-43 0.0 7.750508045802403E-5 0.0 0.2995958885104919 8.138033448092522E-4 44-45 0.0 7.750508045802403E-5 0.0 0.3817900263362264 8.913084252672762E-4 46-47 0.0 7.750508045802403E-5 0.0 0.47762505832257307 9.688135057253003E-4 48-49 0.0 7.750508045802403E-5 0.0 0.580784320412203 0.0010850711264123363 50-51 0.0 7.750508045802403E-5 0.0 0.6860362196741996 0.0011625762068703603 52-53 0.0 7.750508045802403E-5 0.0 0.7852427226604703 0.0013175863677864085 54-55 0.0 7.750508045802403E-5 0.0 0.8971213063016281 0.0013175863677864085 56-57 0.0 7.750508045802403E-5 0.0 1.0339565258502694 0.0013563389080154206 58-59 0.0 7.750508045802403E-5 0.0 1.1689316234679183 0.0013950914482444325 60-61 0.0 7.750508045802403E-5 0.0 1.3128198053382398 0.0013950914482444325 62-63 0.0 7.750508045802403E-5 0.0 1.4548478652775692 0.0013950914482444325 64-65 0.0 7.750508045802403E-5 0.0 1.5983485217456004 0.0013950914482444325 66-67 0.0 7.750508045802403E-5 0.0 1.76467442440852 0.0014338439884734443 68-69 0.0 1.5501016091604806E-4 0.0 1.95568569519732 0.0014725965287024564 70-71 0.0 1.5501016091604806E-4 0.0 2.1473557591700136 0.0015113490689314685 72-73 0.0 1.5501016091604806E-4 0.0 2.3347630437175155 0.0016663592298475165 74-75 0.0 1.5501016091604806E-4 0.0 2.5279832092993697 0.0017826168505345526 76-77 0.0 1.5501016091604806E-4 0.0 2.72841134736382 0.0019376270114506008 78-79 0.0 1.5501016091604806E-4 0.0 2.9579426431402576 0.002092637172366649 80-81 0.0 1.5501016091604806E-4 0.0 3.203246222789904 0.0021701422528246726 82-83 0.0 1.5501016091604806E-4 0.0 3.4510299650142064 0.0022476473332826968 84-85 0.0 1.5501016091604806E-4 0.0 3.697844893732784 0.0022476473332826968 86-87 0.0 1.5501016091604806E-4 0.0 3.9533403914626604 0.002325152413740721 88-89 0.0 1.5501016091604806E-4 0.0 4.231002342203531 0.0024026574941987447 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2510 0.0 49.71558 1 GTATCAA 4205 0.0 40.662434 1 ATCAACG 5235 0.0 32.5305 3 TATCAAC 5285 0.0 32.221485 2 TCAACGC 5305 0.0 32.101254 4 CAACGCA 5365 0.0 31.742249 5 AACGCAG 5510 0.0 30.904522 6 ACGCAGA 6250 0.0 27.0869 7 CGCAGAG 6290 0.0 27.059542 8 GTACATG 3620 0.0 25.12984 1 TGGTATC 1205 0.0 24.869154 2 TACATGG 3615 0.0 24.605986 2 GCAGAGT 6820 0.0 23.911003 9 GAGTACT 4625 0.0 23.232805 12-13 GTGGTAT 1430 0.0 22.98148 1 ACATGGG 3690 0.0 22.688755 3 GTACTTT 4840 0.0 21.854404 14-15 CAGAGTA 6630 0.0 21.08126 10-11 TAAGGTG 665 0.0 20.744392 5 GTATAAG 460 0.0 20.70795 1 >>END_MODULE