FastQCFastQC Report
Thu 26 May 2016
SRR938060_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938060_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1290238
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48820.3783798027960733No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44660.3461376893255353No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27570.21368150682277223No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24450.048.3816381
GTATCAA39900.039.2917251
TCAACGC50400.030.8124164
TATCAAC50850.030.7348652
ATCAACG50800.030.572173
CAACGCA51700.029.945785
GTATACG650.00583996529.2352431
AACGCAG53650.028.8562326
CGTAAAC1004.9575698E-528.4966283
CCGTCCG700.00840155427.136499
ACGCAGA61450.025.1934387
CGCAGAG62500.024.6942088
TAAGGTG6600.023.0257935
TGGTATC12150.022.6766092
ATACCGA1050.00219936322.613746
GTAAGGT6150.022.3939884
GTGGTAT13150.022.0375921
GTACATG38200.021.5150721
GCAGAGT70050.021.4225279
GAGTACT44950.021.02480312-13
Produced by FastQC (version 0.11.5)