##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938059_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 187880 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.363774749840324 33.0 31.0 34.0 31.0 34.0 2 32.584841388120076 34.0 31.0 34.0 31.0 34.0 3 32.59854162231211 34.0 31.0 34.0 31.0 34.0 4 36.1158292527145 37.0 35.0 37.0 35.0 37.0 5 36.05827655950607 37.0 35.0 37.0 35.0 37.0 6 36.139754098360655 37.0 36.0 37.0 35.0 37.0 7 36.11595699382585 37.0 36.0 37.0 35.0 37.0 8 36.1386629763679 37.0 36.0 37.0 35.0 37.0 9 37.77425484351714 39.0 38.0 39.0 35.0 39.0 10-11 37.778222801788374 39.0 38.0 39.0 35.0 39.0 12-13 37.72774111134767 39.0 38.0 39.0 35.0 39.0 14-15 38.84737332339792 40.0 38.0 41.0 35.0 41.0 16-17 38.761132105599316 40.0 38.0 41.0 34.5 41.0 18-19 38.999446455184156 40.0 38.5 41.0 35.5 41.0 20-21 39.00914147328082 40.0 38.5 41.0 35.0 41.0 22-23 38.87299340004258 40.0 38.0 41.0 35.0 41.0 24-25 38.735094209069615 40.0 38.0 41.0 35.0 41.0 26-27 38.52855013838621 40.0 38.0 41.0 35.0 41.0 28-29 38.342303598041305 40.0 37.5 41.0 34.0 41.0 30-31 38.00257345113903 40.0 37.0 41.0 34.0 41.0 32-33 37.60596923568235 40.0 35.5 41.0 33.0 41.0 34-35 37.34089312327017 40.0 35.0 41.0 33.0 41.0 36-37 37.181230040451354 40.0 35.0 41.0 33.0 41.0 38-39 36.87979561422185 39.0 35.0 41.0 33.0 41.0 40-41 36.6959788162657 39.0 35.0 41.0 33.0 41.0 42-43 36.351325314030234 39.0 35.0 41.0 31.0 41.0 44-45 35.95468650202257 39.0 35.0 41.0 30.5 41.0 46-47 35.390475303385145 39.0 35.0 41.0 28.0 41.0 48-49 34.84110602512242 39.0 35.0 40.5 23.5 41.0 50-51 34.360088886523314 38.5 35.0 40.0 21.0 41.0 52-53 33.92403928039174 38.0 34.5 40.0 16.5 41.0 54-55 33.57072333404301 38.0 34.0 40.0 8.5 41.0 56-57 33.13376091121992 38.0 33.0 40.0 2.0 41.0 58-59 32.559410261869274 37.5 33.0 40.0 2.0 41.0 60-61 31.944653502235468 37.0 32.0 40.0 2.0 41.0 62-63 31.342660208643814 36.0 31.0 39.5 2.0 41.0 64-65 30.76190387481371 36.0 30.5 39.0 2.0 40.0 66-67 30.10166063444752 35.5 29.5 39.0 2.0 40.0 68-69 29.44908186076219 35.0 28.5 38.5 2.0 40.0 70-71 28.842535128805622 35.0 27.0 38.0 2.0 40.0 72-73 28.231975196934215 34.5 26.0 37.0 2.0 39.0 74-75 27.674579518841814 34.0 26.0 36.5 2.0 39.0 76-77 26.38932297210986 32.0 24.0 35.0 2.0 37.5 78-79 27.016465296998085 34.0 25.5 35.0 2.0 38.0 80-81 26.747511709601874 34.0 24.5 35.0 2.0 37.0 82-83 26.289128699169684 33.0 22.0 35.0 2.0 37.0 84-85 25.77079518841814 33.0 20.0 35.0 2.0 36.0 86-87 25.25352884820098 33.0 17.5 35.0 2.0 36.0 88-89 24.64229295294869 32.0 9.0 35.0 2.0 35.0 90-91 24.150697253566108 32.0 2.0 35.0 2.0 35.0 92-93 23.425659995741963 31.0 2.0 34.0 2.0 35.0 94-95 22.748448477751758 31.0 2.0 34.0 2.0 35.0 96-97 21.92051309346391 30.5 2.0 34.0 2.0 35.0 98-99 20.796612199276133 29.5 2.0 34.0 2.0 35.0 100-101 17.16794230359804 22.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20989962787894 1101 2 0.07030191001731367 1101 3 0.3113268380657175 1101 4 -0.09064475839742414 1101 5 -0.18956762441550978 1101 6 0.11659296767083305 1101 7 0.02676744648920959 1101 8 0.2929295492335342 1101 9 0.3844285271730925 1101 10-11 0.31967755352367533 1101 12-13 0.24439981239893882 1101 14-15 0.1705558870000985 1101 16-17 0.20116119033123425 1101 18-19 0.38933422411817276 1101 20-21 0.22365173439389707 1101 22-23 0.3914278726090359 1101 24-25 0.5803743851993417 1101 26-27 0.5552846573259203 1101 28-29 0.4611820522101411 1101 30-31 0.42645738287331625 1101 32-33 0.05608595827369811 1101 34-35 0.09508253032215919 1101 36-37 0.15059568409201773 1101 38-39 0.10818864040678022 1101 40-41 0.22746690833128724 1101 42-43 0.22084778048067477 1101 44-45 0.35164731904686164 1101 46-47 0.6031229554333919 1101 48-49 0.6574776779365195 1101 50-51 -0.33070315411060136 1101 52-53 -0.3900466929158952 1101 54-55 -0.0101426366842432 1101 56-57 -0.10199114910076901 1101 58-59 0.07623524999272746 1101 60-61 0.29194331309725996 1101 62-63 0.09086764532979075 1101 64-65 0.48174089130430175 1101 66-67 0.024670619198815302 1101 68-69 0.2248857089477383 1101 70-71 0.729354402418064 1101 72-73 0.5560181841057386 1101 74-75 0.5490498418837078 1101 76-77 0.44483471366405425 1101 78-79 0.534657798032061 1101 80-81 0.4562637046642095 1101 82-83 0.8600095285797877 1101 84-85 0.43644408990367367 1101 86-87 0.7367446726763802 1101 88-89 1.1529949694847375 1101 90-91 1.220159132402788 1101 92-93 1.6253795042346795 1101 94-95 1.9047015603437885 1101 96-97 2.057306844813972 1101 98-99 2.92632433040599 1101 100-101 3.6154902038860577 1102 1 0.3719394468109627 1102 2 0.1998536465874352 1102 3 0.2492349420596227 1102 4 0.3554154106527676 1102 5 0.18270333660679938 1102 6 0.20756761014169456 1102 7 0.35156815153267473 1102 8 0.41907624634526286 1102 9 0.4004923015591828 1102 10-11 0.4309622548816847 1102 12-13 0.5332673363343758 1102 14-15 0.6915669834227955 1102 16-17 0.5595716377934821 1102 18-19 0.5187858124689342 1102 20-21 0.42416849920581967 1102 22-23 0.5538482547746426 1102 24-25 0.6166881295409681 1102 26-27 0.7611893963396668 1102 28-29 0.365300146990414 1102 30-31 0.485965454819798 1102 32-33 0.3989534020905481 1102 34-35 0.8910004889008576 1102 36-37 0.8188647222685148 1102 38-39 0.8559753647588195 1102 40-41 0.8880445632605856 1102 42-43 0.6408694124960519 1102 44-45 0.6302196060316021 1102 46-47 0.37384362201994037 1102 48-49 -0.005403386038167923 1102 50-51 -0.5531724086072458 1102 52-53 -0.4960836275791962 1102 54-55 -1.0250647213695672 1102 56-57 -1.4188000262389266 1102 58-59 -1.5935360114742387 1102 60-61 -1.8229467241578767 1102 62-63 -1.538724150528079 1102 64-65 -1.5977567652273663 1102 66-67 -1.4812180960998234 1102 68-69 -1.7470928192740303 1102 70-71 -1.8862767316603737 1102 72-73 -1.568265756125804 1102 74-75 -1.5681035451290732 1102 76-77 -1.3560706262389992 1102 78-79 -1.5300980406091291 1102 80-81 -1.4533825718249922 1102 82-83 -1.0837071721372773 1102 84-85 -1.5962442888969512 1102 86-87 -1.1606234321187188 1102 88-89 -1.025549275998575 1102 90-91 -0.6101296950618611 1102 92-93 -0.3238653781913108 1102 94-95 -0.3005422004221465 1102 96-97 -0.43003442025833394 1102 98-99 0.4231997059618138 1102 100-101 1.3786596947325087 1103 1 0.16801460006308844 1103 2 0.05953838506367504 1103 3 -0.08067509695001007 1103 4 -0.20481068599558228 1103 5 -0.06238065352137312 1103 6 0.05793780359762479 1103 7 0.05095514301424231 1103 8 0.06842741265367636 1103 9 0.02225808453824385 1103 10-11 0.14149485578926146 1103 12-13 0.0479755602018912 1103 14-15 0.2681532424786113 1103 16-17 -0.02672722376212988 1103 18-19 -0.15154298300913638 1103 20-21 0.0318231445366024 1103 22-23 -0.0031137705418089467 1103 24-25 0.16351959200414257 1103 26-27 -0.18604808475260626 1103 28-29 0.2847731188875571 1103 30-31 0.41051365958056607 1103 32-33 0.5672122986752157 1103 34-35 0.3832184651766326 1103 36-37 0.37292092798462306 1103 38-39 0.6047015808244183 1103 40-41 0.35731452584317935 1103 42-43 0.2138333484884143 1103 44-45 -0.030336878324746408 1103 46-47 -0.0011663294170460858 1103 48-49 -0.29156690805074703 1103 50-51 -0.2321987600124018 1103 52-53 -0.10795768219268354 1103 54-55 0.08836175741396346 1103 56-57 0.2243613462632581 1103 58-59 0.43385034311536685 1103 60-61 0.5537549715669918 1103 62-63 0.914902153110166 1103 64-65 0.07623415906209985 1103 66-67 0.1514544618175364 1103 68-69 0.5063901836715985 1103 70-71 0.14427417989125502 1103 72-73 -0.3403778491120306 1103 74-75 -0.5842646787967745 1103 76-77 -0.22081069470488757 1103 78-79 -0.32229213343612884 1103 80-81 -0.40491907369586144 1103 82-83 0.16364574562404854 1103 84-85 0.4643002537350007 1103 86-87 0.3801575566560622 1103 88-89 -0.022285204666676606 1103 90-91 -0.3510937902772078 1103 92-93 -0.19401902837839913 1103 94-95 -0.25711412940868783 1103 96-97 0.8358281702997452 1103 98-99 1.007030611144522 1103 100-101 0.825439409723355 1104 1 0.15833029321544956 1104 2 0.27327942517347736 1104 3 0.2726114670274242 1104 4 0.11812663290024972 1104 5 0.06260028288247099 1104 6 -0.03646512457073925 1104 7 -0.11543192424212378 1104 8 -0.03382971713128313 1104 9 0.016920277614346446 1104 10-11 -0.0018633873097257947 1104 12-13 0.03768121827723547 1104 14-15 0.21443202850880283 1104 16-17 0.0562375467121754 1104 18-19 0.40965116065412843 1104 20-21 0.4809995971826311 1104 22-23 0.43637837525657375 1104 24-25 0.0998471808405057 1104 26-27 -0.2168548561419641 1104 28-29 0.007245286037175447 1104 30-31 -0.19807258412845385 1104 32-33 0.25681882605054085 1104 34-35 0.1175481891357606 1104 36-37 0.31412879743712097 1104 38-39 0.5060284071169292 1104 40-41 0.6680892703909933 1104 42-43 1.0493145136554105 1104 44-45 0.6457344957792728 1104 46-47 0.3031267924374532 1104 48-49 0.25963791122635627 1104 50-51 0.5221477399113539 1104 52-53 0.05049892906246356 1104 54-55 -0.6452435498912052 1104 56-57 -0.11962218278966219 1104 58-59 -0.14045955470376015 1104 60-61 -0.2363929619391918 1104 62-63 -0.6002343422383127 1104 64-65 -0.7359131644089985 1104 66-67 -0.5157714036697243 1104 68-69 -0.029334995526205887 1104 70-71 -0.4332002748898951 1104 72-73 -0.4661213293621458 1104 74-75 -0.38512635334305756 1104 76-77 -0.2994289652554407 1104 78-79 -0.27005787996655783 1104 80-81 -0.19495262562510263 1104 82-83 -0.3823356483060998 1104 84-85 0.2563926740491702 1104 86-87 0.5014020282131746 1104 88-89 0.41957082705514637 1104 90-91 0.7513539754693213 1104 92-93 0.8110137610069934 1104 94-95 0.8317984830665246 1104 96-97 1.6510256691559277 1104 98-99 1.9128564461300357 1104 100-101 1.320597256299532 1105 1 -0.09155058623455403 1105 2 -0.05406384621716853 1105 3 0.05863692479067595 1105 4 0.0858279466297418 1105 5 -0.17877198585348708 1105 6 0.0355177220700611 1105 7 0.10266078558021263 1105 8 0.13232643289254042 1105 9 0.35358898634758873 1105 10-11 0.4238734583781181 1105 12-13 0.2856391517058654 1105 14-15 0.2689041543001096 1105 16-17 0.039424531941222085 1105 18-19 -0.17378427600776547 1105 20-21 -0.008994276617656283 1105 22-23 0.07927198358820675 1105 24-25 0.369859433239931 1105 26-27 0.19307299083917684 1105 28-29 0.20156493752447346 1105 30-31 0.42712311391797186 1105 32-33 -0.1605285294732539 1105 34-35 -0.14497648583531486 1105 36-37 -0.699416910819636 1105 38-39 -0.3919193159788463 1105 40-41 -0.20858692591966843 1105 42-43 -0.8959070505675797 1105 44-45 -0.42840953798290116 1105 46-47 -0.12317162155750339 1105 48-49 0.10391331793795189 1105 50-51 -0.15683939506924816 1105 52-53 0.09809609739681946 1105 54-55 0.2173934387412899 1105 56-57 1.0379743048558367 1105 58-59 0.8929792854024257 1105 60-61 0.35592546430817507 1105 62-63 0.33069275598508696 1105 64-65 0.6715642026865005 1105 66-67 0.6836363970245749 1105 68-69 1.1828187040177305 1105 70-71 1.4448713335778756 1105 72-73 1.26160312778849 1105 74-75 1.171796252333884 1105 76-77 1.563820643348464 1105 78-79 1.3117098689953934 1105 80-81 1.1746954582357674 1105 82-83 0.8769047029293482 1105 84-85 1.0156840769489044 1105 86-87 1.5033249742345483 1105 88-89 1.3626822558210563 1105 90-91 1.6345137331440895 1105 92-93 1.6971345152102444 1105 94-95 1.6796304890565885 1105 96-97 2.142772316763306 1105 98-99 2.4971257266419116 1105 100-101 1.897634217704475 1106 1 0.20955157177229466 1106 2 -0.06749350260319886 1106 3 -0.16049802010142145 1106 4 0.07332447344274584 1106 5 -0.083097261392993 1106 6 -0.0033819722894818938 1106 7 -0.009499999823141536 1106 8 0.23689251606379003 1106 9 0.21067891873621392 1106 10-11 0.25151076503736647 1106 12-13 0.2640127740083571 1106 14-15 0.39968122200409795 1106 16-17 0.2303108892627037 1106 18-19 -0.07199674243362608 1106 20-21 -0.4104020750801922 1106 22-23 -0.23030475068983236 1106 24-25 -0.2717539778927929 1106 26-27 -0.0015180992784422642 1106 28-29 -0.16279553297658822 1106 30-31 -0.16915362962190272 1106 32-33 -0.15589761347806785 1106 34-35 -0.13900260420152222 1106 36-37 0.4309896836047429 1106 38-39 -0.1668104894529563 1106 40-41 0.08218828941792111 1106 42-43 -0.27814285681005657 1106 44-45 -0.07562635746979396 1106 46-47 0.20191868130439872 1106 48-49 0.1701817258290319 1106 50-51 0.38326433744904165 1106 52-53 0.3084323018980726 1106 54-55 0.3404368767333281 1106 56-57 -0.10660289251380561 1106 58-59 0.17231613823191338 1106 60-61 -0.19973814595394046 1106 62-63 -0.16249979750199017 1106 64-65 0.28052965605317937 1106 66-67 0.5046051846507709 1106 68-69 0.6315544639510478 1106 70-71 0.6746573852589002 1106 72-73 0.5292700723101191 1106 74-75 0.31322442682679963 1106 76-77 0.5788711203328099 1106 78-79 0.9316505932706534 1106 80-81 0.6134284396194865 1106 82-83 1.129613788786532 1106 84-85 0.9278819096802202 1106 86-87 1.4233027458377734 1106 88-89 1.0297657049272892 1106 90-91 1.4266782232802377 1106 92-93 0.9145097120241772 1106 94-95 1.5509373335504328 1106 96-97 1.6101243589972576 1106 98-99 1.4511407308097404 1106 100-101 1.3311805731735866 1107 1 0.3765956197148128 1107 2 0.39176441485596314 1107 3 0.38054596746009395 1107 4 0.3020779076123574 1107 5 -0.14374998004770134 1107 6 -0.029375994570074226 1107 7 0.1578157626379877 1107 8 0.38512788121791175 1107 9 0.5150897510883539 1107 10-11 0.3463693225351818 1107 12-13 0.38616354537568043 1107 14-15 0.48044081503612546 1107 16-17 0.2113663492150053 1107 18-19 0.2622272107107051 1107 20-21 0.24070138777039318 1107 22-23 0.25274328239895283 1107 24-25 0.7256043531762586 1107 26-27 0.6291312984210364 1107 28-29 0.5056976590651914 1107 30-31 0.7277910915688182 1107 32-33 0.5985781284535179 1107 34-35 0.8387922815274322 1107 36-37 0.8995311804498272 1107 38-39 0.9218741103719381 1107 40-41 0.8980866078069525 1107 42-43 0.9053852052616165 1107 44-45 0.862785018311385 1107 46-47 0.06464246840097587 1107 48-49 0.02058778808184769 1107 50-51 -0.1813735274786481 1107 52-53 -0.043739904725725864 1107 54-55 -0.4381610763506316 1107 56-57 -0.47476096998263273 1107 58-59 -0.08356346204583787 1107 60-61 -0.36115166526076337 1107 62-63 0.13914860391092176 1107 64-65 0.25974349537932895 1107 66-67 0.08137963140981697 1107 68-69 0.194305422499486 1107 70-71 0.5131985801989707 1107 72-73 0.5359421732671592 1107 74-75 0.664913585417505 1107 76-77 0.5214789759014486 1107 78-79 0.33546365368184894 1107 80-81 0.3065117955956964 1107 82-83 0.5334268491977276 1107 84-85 0.43716548906614605 1107 86-87 0.749571501687182 1107 88-89 0.8703296635059239 1107 90-91 0.4566724870848411 1107 92-93 -0.05505826189122942 1107 94-95 0.011855728623341832 1107 96-97 1.0139525146594899 1107 98-99 1.4873089522872611 1107 100-101 2.5581829582232487 1108 1 0.39320159661085086 1108 2 0.39239224006942663 1108 3 0.5170665601270912 1108 4 0.3825023174566553 1108 5 0.15597377685837444 1108 6 0.332031589558504 1108 7 0.18933258835371447 1108 8 0.39545560597933616 1108 9 0.39445313632190704 1108 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 12.0 6 23.0 7 65.0 8 119.0 9 201.0 10 329.0 11 553.0 12 886.0 13 1358.0 14 1531.0 15 2066.0 16 2828.0 17 3706.0 18 3724.0 19 2661.0 20 2435.0 21 2480.0 22 2901.0 23 2902.0 24 3071.0 25 3070.0 26 2911.0 27 2627.0 28 3152.0 29 3666.0 30 4550.0 31 5337.0 32 6764.0 33 8750.0 34 12585.0 35 19290.0 36 23011.0 37 34077.0 38 23010.0 39 1227.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.59335746220992 24.635937832659145 16.71971471151799 19.050989993612944 2 14.424121116623725 25.499861593169094 39.444349807295104 20.631667482912082 3 17.588196470012136 31.244544273882774 33.41352807170687 17.75373118439822 4 10.591084497671325 23.299001996007984 38.41756487025948 27.692348636061208 5 8.953426409463924 41.82084621123308 36.28370457209847 12.94202280720452 6 23.241430700447093 42.30998509687034 17.325420481158186 17.123163721524374 7 20.04204811581861 39.06536086863956 23.870023419203747 17.022567596338085 8 22.47764530551416 35.47423887587822 22.19980838833298 19.84830743027464 9 22.554289972322756 19.671598892910367 23.700766446668087 34.073344688098786 10-11 21.03869491164573 30.249893549073875 27.954545454545453 20.756866084734938 12-13 24.057377049180328 29.311794762614436 28.444485842026822 18.18634234617841 14-15 21.35358035336479 31.245026732559968 28.31868298563707 19.082709928438174 16-17 16.7026825633383 34.4914307004471 29.81450926123057 18.991377474984034 18-19 16.035501383862037 32.03880136257185 33.82504790291676 18.100649350649352 20-21 17.427347242921012 30.47769853097722 33.72338726846923 18.37156695763253 22-23 17.237112085139678 31.0731080293486 34.2208702918626 17.46890959364912 24-25 18.114850515491355 32.105237945699095 33.64080072812821 16.13911081068134 26-27 16.796278257722136 31.448775476528855 35.35149492465494 16.403451341094062 28-29 15.501117734724293 31.41739408132851 35.6405684479455 17.440919736001703 30-31 16.8211092186502 31.13130721737279 35.23099850968703 16.816585054289973 32-33 15.855599318714072 32.71636150734511 35.068394720034064 16.35964445390675 34-35 16.107980933269104 32.145699206617465 35.46374686279885 16.28257299731458 36-37 16.42612872749531 31.384545781160593 34.73806070444837 17.451264786895717 38-39 15.535086481983695 31.27616482542589 35.650963557538 17.537785135052417 40-41 15.327987310070238 31.349289784446853 35.910265588945286 17.41245731653763 42-43 16.20810977686936 30.847066087548967 34.74812638392097 18.196697751660707 44-45 15.701788703569155 30.682879703630366 35.03900230740365 18.576329285396824 46-47 17.473919523099852 30.489142005535445 34.449648711943794 17.58728975942091 48-49 16.529167553757716 30.35022354694486 34.390036193314884 18.730572705982542 50-51 15.11762827336598 30.532786885245898 35.30844155844156 19.041143282946564 52-53 15.790664253779008 31.611134766872475 34.612252501596764 17.985948477751755 54-55 16.05812220566319 30.361933148818395 33.79896742601661 19.78097721950181 56-57 17.387428145624867 30.698051948051948 32.08297849691292 19.83154140941026 58-59 16.661432829465618 31.143815201192247 33.528315946348734 18.6664360229934 60-61 16.84639131360443 33.17383436235895 31.589844581647856 18.38992974238876 62-63 15.567117308920588 33.626250798381946 32.03959974451778 18.767032148179688 64-65 15.92825207579306 33.30663189269747 31.385991058122205 19.379124973387267 66-67 16.041132747319427 32.71618244672782 30.569593808797663 20.673090997155093 68-69 16.033628384528175 31.615631503590112 31.486557694661933 20.864182417219777 70-71 17.31025122418565 30.984138812007668 31.116670215030872 20.588939748775815 72-73 16.286459442197145 30.36432829465616 32.01910794123909 21.3301043219076 74-75 16.076751117734727 31.339951032573982 30.900574835001066 21.68272301469023 76-77 16.110815414094105 30.18921652118373 32.03933361720247 21.660634447519694 78-79 16.204492229082394 29.89487971045348 32.11810730253353 21.782520757930595 80-81 17.08142719136468 29.99316050979484 30.97943096506005 21.94598133378043 82-83 16.74109519044476 29.438831995571547 31.629904830845877 22.190167983137815 84-85 16.18656021290752 29.37005988023952 31.599733865602133 22.84364604125083 86-87 16.27048547720051 29.845058202973224 31.389419673509796 22.495036646316475 88-89 16.356717053438366 29.500479029167554 30.574568873749204 23.56823504364488 90-91 16.77099135063207 28.288489687292085 31.23273453093812 23.707784431137725 92-93 17.23147753885459 29.068022141792632 30.89418777943368 22.806312539919098 94-95 16.4972322759208 27.910900574835 31.695497125824996 23.896370023419202 96-97 16.227954619849424 28.454408277646042 31.44834854254988 23.86928855995465 98-99 16.50282094954226 28.645944219714714 30.837502661273152 24.013732169469876 100-101 16.19916968277624 27.806312539919098 31.360708963167976 24.63380881413668 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3242.0 1 1845.0 2 521.5 3 653.0 4 508.5 5 396.5 6 743.5 7 1312.5 8 1615.0 9 1575.0 10 1227.0 11 777.0 12 626.0 13 613.5 14 621.5 15 590.5 16 568.0 17 591.0 18 610.5 19 662.0 20 714.5 21 781.5 22 872.5 23 951.0 24 1033.5 25 1163.0 26 1419.0 27 1790.0 28 2010.0 29 2308.0 30 3028.0 31 3592.0 32 4481.5 33 5653.5 34 6188.5 35 7049.0 36 8917.5 37 10039.5 38 9716.5 39 9046.0 40 8379.0 41 7443.5 42 6263.0 43 5945.5 44 6371.0 45 6576.5 46 6110.5 47 6435.0 48 7935.5 49 7555.0 50 5997.5 51 4503.5 52 2761.5 53 1599.5 54 1086.5 55 889.5 56 693.0 57 571.0 58 512.5 59 450.0 60 363.0 61 281.5 62 221.5 63 148.0 64 95.5 65 70.5 66 41.0 67 40.0 68 34.0 69 18.0 70 12.5 71 8.5 72 6.0 73 4.5 74 2.5 75 3.5 76 5.0 77 4.5 78 4.0 79 2.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014903129657228018 3 0.0021290185224611454 4 0.0026612731530764318 5 0.117096018735363 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 2.661273153076432E-4 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.661273153076432E-4 24-25 5.322546306152864E-4 26-27 0.0058548009367681494 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0071854375133063655 36-37 0.0013306365765382159 38-39 0.004524164360229934 40-41 0.007717692143921652 42-43 0.004258037044922291 44-45 0.0034596550989993615 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.661273153076432E-4 68-69 0.001596763891845859 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.661273153076432E-4 82-83 0.0021290185224611454 84-85 0.0026612731530764318 86-87 0.001596763891845859 88-89 0.0 90-91 0.0026612731530764318 92-93 0.0 94-95 0.0 96-97 2.661273153076432E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 187880.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.95039386842665 #Duplication Level Percentage of deduplicated Percentage of total 1 77.730222932932 26.389716840536515 2 9.32493023547487 6.3317010857994465 3 3.5932649797761265 3.659782840110709 4 2.0004389677985768 2.716627634660422 5 1.2683033894585019 2.152969980838833 6 0.8606904336374753 1.753246753246753 7 0.6333678236603644 1.5052160953800298 8 0.5189226476029223 1.4094102618692783 9 0.3872323080299752 1.1832020438577815 >10 3.1072649170664413 21.56536086863956 >50 0.39193553444329476 9.139876516925698 >100 0.15990969805286426 9.841388120076644 >500 0.009406452826639074 2.2610176708537364 >1k 0.014109679239958612 10.090483287204599 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3605 1.9187779433681071 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3031 1.613263785394933 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2924 1.5563125399190973 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2259 1.2023632105599318 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1986 1.0570576964019587 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1544 0.8218011496700022 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1353 0.7201405152224825 TruSeq Adapter, Index 11 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1212 0.6450926123057271 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1044 0.555673834362359 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 867 0.46146476474345327 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 758 0.40344901000638705 RNA PCR Primer, Index 11 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 722 0.38428784330423676 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 689 0.3667234404939323 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 658 0.35022354694485847 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 554 0.29486906536086865 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 483 0.2570789865871833 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 481 0.2560144773259527 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 422 0.22461145411965086 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 422 0.22461145411965086 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 411 0.21875665318288268 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 409 0.21769214392165212 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 384 0.20438577815626996 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 383 0.20385352352565467 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 349 0.18575686608473496 TruSeq Adapter, Index 11 (96% over 28bp) CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 340 0.18096657440919736 TruSeq Adapter, Index 11 (96% over 26bp) GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 312 0.16606344475196935 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 311 0.16553119012135406 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 288 0.15328933361720248 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 275 0.14637002341920377 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 273 0.1453055141579732 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 269 0.14317649563551205 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 259 0.13785394932935918 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 258 0.1373216946987439 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 257 0.1367894400681286 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 256 0.1362571854375133 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 250 0.13306365765382158 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 230 0.12241856504151587 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 228 0.12135405578028528 TruSeq Adapter, Index 11 (95% over 24bp) GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 227 0.12082180114967002 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 218 0.11603150947413242 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 207 0.11017670853736429 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 204 0.10857994464551841 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 201 0.10698318075367257 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 200 0.10645092612305727 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 197 0.10485416223121143 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 189 0.10059612518628913 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.983819459229295E-4 0.0 22-23 0.0 0.0 0.0 0.003725782414307005 0.0 24-25 0.0 0.0 0.0 0.012774111134766872 0.0 26-27 0.0 0.0 0.0 0.03779007877368533 0.0 28-29 0.0 0.0 0.0 0.10485416223121141 0.0 30-31 0.0 0.0 0.0 0.26772407919948904 0.0 32-33 0.0 0.0 0.0 0.5197466467958272 0.0 34-35 0.0 0.0 0.0 0.8627847562273792 0.0 36-37 0.0 0.0 0.0 1.3503299978709815 0.0 38-39 0.0 0.0 0.0 1.9121247604854164 0.0 40-41 0.0 0.0 0.0 2.4427826272088566 0.0 42-43 0.0 0.0 0.0 2.919682776240153 0.0 44-45 0.0 0.0 0.0 3.4444858420268254 0.0 46-47 0.0 0.0 0.0 4.017191824568874 0.0 48-49 0.0 0.0 0.0 4.508728975942091 0.0 50-51 0.0 0.0 0.0 4.984830743027464 0.0 52-53 0.0 0.0 0.0 5.421279540132 0.0 54-55 0.0 0.0 0.0 5.902703853523526 0.0 56-57 0.0 0.0 0.0 6.496700021290184 0.0 58-59 0.0 0.0 0.0 7.023365978284011 0.0 60-61 0.0 0.0 0.0 7.55535448158399 0.0 62-63 0.0 0.0 0.0 8.038907813497978 0.0 64-65 0.0 0.0 0.0 8.53896103896104 0.0 66-67 0.0 0.0 0.0 9.09090909090909 0.0 68-69 0.0 0.0 0.0 9.70433255269321 0.0 70-71 0.0 0.0 0.0 10.32547370662125 0.0 72-73 0.0 0.0 0.0 10.900308707685756 0.0 74-75 0.0 0.0 0.0 11.535554609325102 0.0 76-77 0.0 0.0 0.0 12.161752182243987 0.0 78-79 0.0 0.0 0.0 12.885352352565468 0.0 80-81 0.0 0.0 0.0 13.600702576112411 0.0 82-83 0.0 0.0 0.0 14.251916116670216 0.0 84-85 0.0 0.0 0.0 14.934266553119013 0.0 86-87 0.0 0.0 0.0 15.599850968703429 0.0 88-89 0.0 0.0 0.0 16.256919310198 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 85 1.2732926E-10 50.28862 9 GAGATGT 40 5.3583493E-4 47.532764 1 GTACCCA 50 0.0016069202 38.02621 1 TAGTATG 65 1.3185947E-4 36.563667 5 TAAGGTG 55 0.0025654628 34.569286 5 GTGTATA 55 0.002575538 34.54168 6 CATAGTG 105 1.8803403E-6 31.68851 4 TATAAGA 60 0.0039447257 31.663204 9 ATATAGG 85 6.361369E-4 27.960451 2 CTAAGAA 35 0.0083889775 27.13989 12-13 TCCTCAT 90 8.883734E-4 26.407091 2 ATAGTGG 120 1.695453E-4 23.766382 5 TATAGGA 165 2.397519E-6 23.04619 2 GTATAGG 170 3.1071231E-6 22.36836 1 GGTATCA 3685 0.0 20.767368 1 GAGTACT 2005 0.0 20.72716 12-13 ATGGGAG 115 0.003697052 20.66642 5 GTACTTT 1920 0.0 20.531609 14-15 GATGACC 70 4.8382545E-4 20.360338 22-23 TAAGGCT 240 1.3873796E-7 19.805319 4 >>END_MODULE