##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938058_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 185762 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.38817411526577 33.0 31.0 34.0 31.0 34.0 2 32.61168592069422 34.0 31.0 34.0 31.0 34.0 3 32.523357844984446 34.0 31.0 34.0 31.0 34.0 4 36.094098900743965 37.0 35.0 37.0 35.0 37.0 5 36.07551059958441 37.0 35.0 37.0 35.0 37.0 6 36.16428548357576 37.0 36.0 37.0 35.0 37.0 7 36.133773322853976 37.0 36.0 37.0 35.0 37.0 8 36.15020294785801 37.0 36.0 37.0 35.0 37.0 9 37.80749561266567 39.0 38.0 39.0 35.0 39.0 10-11 37.791868089275525 39.0 38.0 39.0 35.0 39.0 12-13 37.76208535653148 39.0 38.0 39.0 35.0 39.0 14-15 38.89765129574402 40.0 38.0 41.0 35.0 41.0 16-17 38.823365381509674 40.0 38.0 41.0 35.0 41.0 18-19 38.871397271777866 40.0 38.0 41.0 35.0 41.0 20-21 38.93231931180758 40.0 38.0 41.0 35.0 41.0 22-23 38.880513775691476 40.0 38.0 41.0 35.0 41.0 24-25 38.779012930524004 40.0 38.0 41.0 35.0 41.0 26-27 38.53493717767897 40.0 38.0 41.0 35.0 41.0 28-29 38.40555926400448 40.0 38.0 41.0 34.5 41.0 30-31 38.076296551501386 40.0 37.0 41.0 34.0 41.0 32-33 37.6956428117699 40.0 35.5 41.0 33.5 41.0 34-35 37.38848365112348 40.0 35.0 41.0 33.0 41.0 36-37 37.19248554602125 40.0 35.0 41.0 33.0 41.0 38-39 36.973234568964585 40.0 35.0 41.0 33.0 41.0 40-41 36.83795663268053 40.0 35.0 41.0 33.0 41.0 42-43 36.56064749518201 40.0 35.0 41.0 31.5 41.0 44-45 36.16077830772709 40.0 35.0 41.0 30.5 41.0 46-47 35.60253442577061 39.5 35.0 41.0 28.5 41.0 48-49 35.06836435869553 39.0 35.0 41.0 24.5 41.0 50-51 34.645928661405456 39.0 35.0 41.0 22.5 41.0 52-53 34.2610598507768 39.0 35.0 40.0 19.0 41.0 54-55 33.934642176548486 38.0 35.0 40.0 10.5 41.0 56-57 33.57451200999128 38.0 34.0 40.0 3.5 41.0 58-59 33.09584306801176 38.0 33.5 40.0 2.0 41.0 60-61 32.522135851250525 38.0 33.0 40.0 2.0 41.0 62-63 32.019185839945735 37.0 32.5 40.0 2.0 41.0 64-65 31.464801735554097 36.5 31.5 39.5 2.0 41.0 66-67 30.784708390305873 36.0 31.0 39.0 2.0 40.5 68-69 30.122333954199462 35.5 30.0 39.0 2.0 40.0 70-71 29.469786608671313 35.0 29.0 38.5 2.0 40.0 72-73 29.01319699400308 35.0 28.5 37.5 2.0 39.5 74-75 28.515124191169345 35.0 27.5 37.0 2.0 39.0 76-77 27.28991397594772 33.5 26.0 35.5 2.0 38.0 78-79 27.87946942862372 34.0 27.0 36.0 2.0 38.5 80-81 27.703007073567253 34.0 26.5 36.0 2.0 37.5 82-83 27.320584942022588 34.0 26.0 35.0 2.0 37.0 84-85 26.89076345000592 34.0 25.5 35.0 2.0 36.5 86-87 26.503189565142492 34.0 24.5 35.0 2.0 36.0 88-89 26.007224297757347 33.0 22.5 35.0 2.0 36.0 90-91 25.605699766367717 33.0 20.0 35.0 2.0 35.0 92-93 25.157351880363045 33.0 17.5 35.0 2.0 35.0 94-95 24.617825497141503 32.0 4.5 35.0 2.0 35.0 96-97 24.029349382543252 32.0 2.0 34.5 2.0 35.0 98-99 23.346938555786437 32.0 2.0 34.0 2.0 35.0 100-101 20.366697709972975 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16260774706632475 1101 2 0.02170710109180618 1101 3 0.273012773860728 1101 4 0.11531843306281786 1101 5 0.1683191309527814 1101 6 0.14110325966109372 1101 7 0.0840239552118831 1101 8 0.2176622473632719 1101 9 0.22818390090439067 1101 10-11 0.2660340781041839 1101 12-13 0.431409300945937 1101 14-15 0.6857567137004992 1101 16-17 0.4835576293948307 1101 18-19 0.3897586224898504 1101 20-21 0.12255870610574249 1101 22-23 0.07547939452099683 1101 24-25 0.30728781889028767 1101 26-27 0.3361724634974834 1101 28-29 0.24615568200447058 1101 30-31 0.3808926073099812 1101 32-33 0.21520399067466656 1101 34-35 -0.19400706867971707 1101 36-37 -0.0898173300035765 1101 38-39 0.04877401347403776 1101 40-41 -0.20263215132839463 1101 42-43 -0.5532440513639827 1101 44-45 -0.20833796487011114 1101 46-47 -0.1750108579704559 1101 48-49 -0.2214549502438885 1101 50-51 -0.10022485615523635 1101 52-53 -0.11829448359817718 1101 54-55 -0.5580527688493646 1101 56-57 -0.17922061156393454 1101 58-59 0.3726160132525038 1101 60-61 0.25233266534974774 1101 62-63 0.3493105847401452 1101 64-65 -0.1975196303047113 1101 66-67 0.3629051529234104 1101 68-69 0.37474011647873695 1101 70-71 0.29501732331456765 1101 72-73 0.780440018841734 1101 74-75 0.5428523222133457 1101 76-77 0.7156893986251376 1101 78-79 0.9359591765832533 1101 80-81 1.2874656116277166 1101 82-83 1.5025614645060763 1101 84-85 1.3319801200687458 1101 86-87 1.4816489065061162 1101 88-89 1.294346638091426 1101 90-91 1.3704589157695715 1101 92-93 1.6540513985111964 1101 94-95 1.7699060347180051 1101 96-97 1.8972918846956937 1101 98-99 1.010030270385215 1101 100-101 1.3453270267604722 1102 1 0.20058501793543115 1102 2 0.252718465657253 1102 3 0.31904335190014166 1102 4 0.23930665970004839 1102 5 0.24202528744039853 1102 6 0.2036901290912354 1102 7 0.2507723813422089 1102 8 0.2087504658245578 1102 9 0.012011878992858271 1102 10-11 0.22707569367118907 1102 12-13 0.18216557598476868 1102 14-15 0.26559482996245976 1102 16-17 0.4208890005010346 1102 18-19 0.2911771491843069 1102 20-21 0.26345070185425357 1102 22-23 0.32895417172646546 1102 24-25 0.11680870843325408 1102 26-27 0.1329184368439016 1102 28-29 -0.10095412095521539 1102 30-31 0.03582679094173358 1102 32-33 -0.0575251402222392 1102 34-35 -0.2230521181441958 1102 36-37 -0.08147786471046459 1102 38-39 -0.14540471271351407 1102 40-41 -0.05658930936944273 1102 42-43 -0.062462430891407905 1102 44-45 -0.24038767462401722 1102 46-47 -0.3854597174259382 1102 48-49 -0.4161242332133881 1102 50-51 -0.304235157847657 1102 52-53 -0.18971137510337144 1102 54-55 -0.41989971513339697 1102 56-57 -0.3661023056200179 1102 58-59 -0.052206159914035766 1102 60-61 -0.44738526450063176 1102 62-63 -0.5791498845591718 1102 64-65 -0.411770304820287 1102 66-67 -0.33797784928409413 1102 68-69 -0.7201826208281084 1102 70-71 -0.43119067276097667 1102 72-73 -0.3033429915417152 1102 74-75 -0.19561959341088553 1102 76-77 0.20718641440471686 1102 78-79 0.35918704020846803 1102 80-81 0.29220766051987823 1102 82-83 0.33869505946969625 1102 84-85 0.3948940273535122 1102 86-87 0.7937452191543031 1102 88-89 0.9441708552445647 1102 90-91 0.9349671325956699 1102 92-93 1.5386683554239617 1102 94-95 1.574439596977836 1102 96-97 1.7421124226729674 1102 98-99 1.6853838800655332 1102 100-101 2.800543280541845 1103 1 0.2714344743570294 1103 2 0.14639821505839734 1103 3 0.3756123587179019 1103 4 0.11907448722907077 1103 5 -0.005348952471159407 1103 6 -0.1128751924492093 1103 7 0.0886201793890109 1103 8 -0.059445590928717706 1103 9 -0.04363580849562965 1103 10-11 0.02369445173273732 1103 12-13 0.11758109100227898 1103 14-15 0.248596488337256 1103 16-17 0.0015965737459211482 1103 18-19 0.5624135597242059 1103 20-21 0.242628885012536 1103 22-23 0.09917713100414005 1103 24-25 -0.2683027712055832 1103 26-27 0.04428751736720926 1103 28-29 -0.3342782410952623 1103 30-31 -0.18538198001827766 1103 32-33 0.04566262465706927 1103 34-35 0.4549007103061484 1103 36-37 0.3939229091977765 1103 38-39 -0.005318108696563684 1103 40-41 0.1883434343195205 1103 42-43 0.25875555326189925 1103 44-45 0.40412126217347577 1103 46-47 0.6360991548792043 1103 48-49 0.7118009556570684 1103 50-51 1.0150019897476952 1103 52-53 1.2475007126062252 1103 54-55 0.9005812135564923 1103 56-57 1.156377886014397 1103 58-59 1.2592919053153722 1103 60-61 1.2767717283130793 1103 62-63 1.3822007695775511 1103 64-65 1.2207614806965736 1103 66-67 1.1819226482283405 1103 68-69 0.8631507125052273 1103 70-71 0.9869961707990029 1103 72-73 0.7365040694496194 1103 74-75 0.3513676923823681 1103 76-77 0.1070850692784937 1103 78-79 0.4708509429171457 1103 80-81 0.6809122855429095 1103 82-83 0.5797582619756838 1103 84-85 0.435938803472915 1103 86-87 0.6665110197807991 1103 88-89 1.5191662486479167 1103 90-91 1.0006111348068352 1103 92-93 1.348600177793596 1103 94-95 1.5743935310113706 1103 96-97 1.7557479487463041 1103 98-99 2.112185059354278 1103 100-101 2.184088517320916 1104 1 0.18009119077493807 1104 2 0.15582252209464542 1104 3 0.3597488192722764 1104 4 0.29613205652545105 1104 5 0.16445914987426136 1104 6 0.13868131074907808 1104 7 0.20678649068965171 1104 8 -0.08345411971336603 1104 9 0.10118295483059825 1104 10-11 0.09254306580523064 1104 12-13 0.28981989697242483 1104 14-15 0.1319440363116655 1104 16-17 0.3260741856862168 1104 18-19 0.3202600416005339 1104 20-21 0.17881225388247657 1104 22-23 0.3043157237546765 1104 24-25 0.37269936099058043 1104 26-27 0.12850926576805932 1104 28-29 0.30066845400068587 1104 30-31 0.5444687662503824 1104 32-33 0.7072191278553674 1104 34-35 0.7003164885577462 1104 36-37 0.8119612887286891 1104 38-39 0.7963236823482092 1104 40-41 0.5819468458333859 1104 42-43 0.805280073517757 1104 44-45 0.9287481278451253 1104 46-47 0.6656513936851738 1104 48-49 0.3638052200493931 1104 50-51 0.5422727786602763 1104 52-53 0.6114879194292513 1104 54-55 0.8085129832793001 1104 56-57 0.950599634415255 1104 58-59 0.8384816707737954 1104 60-61 1.0684348413194726 1104 62-63 1.2693862706436434 1104 64-65 1.0889704359204586 1104 66-67 0.7639269126206649 1104 68-69 0.6131507125052273 1104 70-71 0.7160580044876994 1104 72-73 0.968103216571155 1104 74-75 0.6895126817213857 1104 76-77 0.9455285660801991 1104 78-79 1.3362240772455038 1104 80-81 1.2819783836239296 1104 82-83 0.9443211617624634 1104 84-85 1.3520368844963677 1104 86-87 1.2655088875846374 1104 88-89 1.4481214725285128 1104 90-91 1.2786708362993728 1104 92-93 1.3768517769407147 1104 94-95 1.3896600555316283 1104 96-97 1.573999547893422 1104 98-99 1.819263950612271 1104 100-101 2.5278625045277394 1105 1 -0.18298573230198656 1105 2 -0.21062436435224186 1105 3 -0.2714599719365154 1105 4 0.11195623234962682 1105 5 -0.29957015415504884 1105 6 -0.025274733206963163 1105 7 -0.023396659493499783 1105 8 0.09574001948077893 1105 9 -0.07478774114009923 1105 10-11 0.05195698521914949 1105 12-13 -0.05582112866859745 1105 14-15 0.02542388979151866 1105 16-17 -0.2990540194419893 1105 18-19 -0.33270699136649284 1105 20-21 -0.09898994391972593 1105 22-23 -0.18476852532957366 1105 24-25 -0.06415045585924162 1105 26-27 -0.018743481484683855 1105 28-29 -0.286804073471842 1105 30-31 -0.3786081568265445 1105 32-33 -0.1654548648186278 1105 34-35 0.1069098951511549 1105 36-37 0.15071586748327093 1105 38-39 0.07529804132257567 1105 40-41 0.34678201066854797 1105 42-43 0.4238148720525601 1105 44-45 0.603107102204099 1105 46-47 0.5179590859928638 1105 48-49 1.0829260991702725 1105 50-51 1.201320397707896 1105 52-53 1.3520740000153353 1105 54-55 1.2241173788837045 1105 56-57 0.9561673999830163 1105 58-59 1.1865402788324033 1105 60-61 0.8835264164110512 1105 62-63 0.6716573329147124 1105 64-65 0.8135858205358346 1105 66-67 1.0286888173825695 1105 68-69 0.7413558407103551 1105 70-71 1.207413315843013 1105 72-73 1.0302277586956983 1105 74-75 0.9606482128569205 1105 76-77 0.7079461484977827 1105 78-79 1.2757112567326843 1105 80-81 1.439121240766788 1105 82-83 1.160584898026201 1105 84-85 1.430791463250948 1105 86-87 1.4187689608447123 1105 88-89 1.0768394212464614 1105 90-91 1.0617477593762956 1105 92-93 1.0629323630213001 1105 94-95 1.097279103150676 1105 96-97 0.8611057749996505 1105 98-99 1.6824873938357143 1105 100-101 2.001635398300632 1106 1 -0.229139578455829 1106 2 -0.024177477905354294 1106 3 -0.17695447743102477 1106 4 -0.045186624793231545 1106 5 -0.04938700397189422 1106 6 -0.01758242551465372 1106 7 -0.132554168651005 1106 8 -0.12037719663643998 1106 9 -0.17222363857599277 1106 10-11 -0.36690748364532055 1106 12-13 -0.2807295535770251 1106 14-15 -0.3824515680839369 1106 16-17 -0.8389807593687308 1106 18-19 -0.37226743092693937 1106 20-21 -0.12316576809554647 1106 22-23 -0.20326669382774298 1106 24-25 0.020281778572993403 1106 26-27 0.06257519983399362 1106 28-29 -0.04614473281250042 1106 30-31 0.09446876625037959 1106 32-33 -0.35080285016661605 1106 34-35 -0.13374944550818668 1106 36-37 0.11317007993748263 1106 38-39 -0.12213785611332639 1106 40-41 -0.43893227504573673 1106 42-43 -0.44175289351520775 1106 44-45 -0.5267463776493884 1106 46-47 -0.8073156392818603 1106 48-49 -0.5764145601703845 1106 50-51 -0.8704744740869685 1106 52-53 -0.6488417509004165 1106 54-55 -0.6170914123250952 1106 56-57 -0.6604992666836438 1106 58-59 -0.8533864610943382 1106 60-61 -0.7096970268123926 1106 62-63 -0.4367675755101956 1106 64-65 -0.2232273662773494 1106 66-67 -0.5705785818848277 1106 68-69 -1.2467393973848822 1106 70-71 -0.9984474900177958 1106 72-73 -1.6932154647475244 1106 74-75 -1.2309268787181722 1106 76-77 -1.1145813240296896 1106 78-79 -1.081797900776472 1106 80-81 -0.8207688691233201 1106 82-83 -1.3877667503254507 1106 84-85 -1.0941169616574768 1106 86-87 -0.9132823212065695 1106 88-89 -0.5218785274714861 1106 90-91 -1.0948456472171095 1106 92-93 -1.0654559153669787 1106 94-95 -0.9102300543584825 1106 96-97 -0.7791872652933876 1106 98-99 -0.07831846697014555 1106 100-101 0.5941262407914749 1107 1 0.1360507867345362 1107 2 0.055187601459721236 1107 3 -0.038981339457883735 1107 4 -0.04352162312822827 1107 5 -0.07447302905791986 1107 6 0.05778664985442106 1107 7 0.1458341097372724 1107 8 0.20762813136888525 1107 9 0.27183230547994697 1107 10-11 0.1821823154444786 1107 12-13 0.29355727070979754 1107 14-15 0.28074634511398244 1107 16-17 -0.21210763249559506 1107 18-19 0.15256884390933578 1107 20-21 0.2606448657150864 1107 22-23 0.03574429518324962 1107 24-25 0.1434641517553672 1107 26-27 -0.2228615856027929 1107 28-29 -0.12340081006858128 1107 30-31 0.20810512988673935 1107 32-33 0.2314759821122152 1107 34-35 0.004501192742452531 1107 36-37 0.09613156289896807 1107 38-39 -0.36397934795481746 1107 40-41 0.19818061206714788 1107 42-43 0.5912973895350788 1107 44-45 0.4484450975420913 1107 46-47 0.48575240378618645 1107 48-49 0.3661428823870594 1107 50-51 0.3952597916472911 1107 52-53 -0.13724223930090318 1107 54-55 -0.15222294745662168 1107 56-57 -0.49966010584448384 1107 58-59 -0.38890649661437493 1107 60-61 0.09891103179565874 1107 62-63 0.06450892576630451 1107 64-65 -0.22911037216035623 1107 66-67 -0.3102000715063191 1107 68-69 -0.6090715097169941 1107 70-71 -1.1955004370707414 1107 72-73 -0.9761824977145572 1107 74-75 -0.7065623543536468 1107 76-77 -0.5383242477726142 1107 78-79 -0.5860991550777257 1107 80-81 -0.9735050218594736 1107 82-83 -0.49912761168630837 1107 84-85 -0.7338938514343667 1107 86-87 -0.6366700445942932 1107 88-89 -0.572383577976538 1107 90-91 -1.341127143498607 1107 92-93 -1.0174483673594281 1107 94-95 -0.9522158463442736 1107 96-97 -0.3981793842855055 1107 98-99 -0.7559741446258244 1107 100-101 0.22618862285385433 1108 1 0.15315651740810665 1108 2 -0.03386879161676859 1108 3 0.1692822004639467 1108 4 0.1971514391528686 1108 5 0.05097745568904344 1108 6 -0.09933735400325361 1108 7 0.22958620353961834 1108 8 0.1704726570274815 1108 9 -2.097228434863041E-4 1108 10-11 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-0.5317380532701144 2316 74-75 -0.7419158897071831 2316 76-77 -0.6570111164594827 2316 78-79 -0.8281348971134683 2316 80-81 -1.2172890156434661 2316 82-83 -1.1218937344524313 2316 84-85 -1.0281828957234111 2316 86-87 -1.3961883041125525 2316 88-89 -1.7953828009757586 2316 90-91 -1.946799249170713 2316 92-93 -2.464418064329127 2316 94-95 -1.9044302985587258 2316 96-97 -2.873509609615734 2316 98-99 -2.918367307018986 2316 100-101 -3.8167406700754363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 3.0 5 10.0 6 14.0 7 64.0 8 114.0 9 155.0 10 357.0 11 539.0 12 872.0 13 1249.0 14 1582.0 15 1982.0 16 2645.0 17 3637.0 18 3497.0 19 2532.0 20 2204.0 21 2328.0 22 2430.0 23 2651.0 24 2887.0 25 2854.0 26 2540.0 27 2198.0 28 2361.0 29 2846.0 30 3432.0 31 4132.0 32 5236.0 33 6697.0 34 9821.0 35 17283.0 36 21076.0 37 37584.0 38 33062.0 39 2886.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.702414918013375 24.513624960971566 16.86351352806279 18.920446592952274 2 14.554362163180704 25.287894694339013 39.518694985060165 20.63904815742012 3 17.773638336016194 30.99861639757304 33.46648936456578 17.761255901844983 4 10.701865828317954 23.318009065363206 38.39536611362927 27.584758992689572 5 8.969589956987742 41.693896996678525 36.30363746965187 13.032875576681866 6 23.130797099498817 42.3398883511609 17.216207923083964 17.31310662625632 7 19.979866711168054 38.97567855643243 23.961305326170045 17.083149406229474 8 22.321034441920308 35.53579311161594 22.171918906988513 19.971253539475242 9 22.575123006858238 19.845285903467875 23.56348445860833 34.016106631065554 10-11 21.00483414261259 30.251881439691648 28.031836435869558 20.711447981826208 12-13 23.980684962478872 29.14966462462721 28.55777823236184 18.31187218053208 14-15 21.357705020402452 31.147112972513213 28.35106211173437 19.144119895349966 16-17 16.584662094508026 34.37436074116342 29.99240964244571 19.048567521882838 18-19 15.967743672010423 31.918261000635223 33.971156641293696 18.142838686060657 20-21 17.31435923385838 30.513237368245388 33.83738331843972 18.33502007945651 22-23 17.243838890623486 30.854534296573032 34.39670115524166 17.504925657561827 24-25 18.032919263017643 32.11256611442337 33.600796737682195 16.25371788487679 26-27 16.78157515715228 31.40030420912358 35.3345627330363 16.48355790068784 28-29 15.424886211620814 31.340724531186492 35.74906533377476 17.48532392341793 30-31 16.881278194679215 31.130424952358393 35.23890784982935 16.749389003133043 32-33 15.550776287172418 32.805669803398004 35.19374878873361 16.449805120695967 34-35 16.064564063358063 31.966802700578235 35.48466118941735 16.48397204664635 36-37 16.33405114401077 31.33512786002692 34.9956931359354 17.335127860026915 38-39 15.483000815702406 31.16548528908978 35.81875792483154 17.532755970376275 40-41 15.2333570244018 31.275440976933517 36.083381792337015 17.407820206327667 42-43 16.116231990179397 30.730030366334287 34.86986625891069 18.283871384575626 44-45 15.75283651193152 30.773496278550184 35.0319654369507 18.441701772567598 46-47 17.25363106837929 30.500705218617775 34.45504365895412 17.790620054048816 48-49 16.464346852424068 30.149869187454915 34.509210710478996 18.876573249642014 50-51 15.110194765344904 30.473401449166136 35.47926917238187 18.937134613107094 52-53 15.668974278916032 31.206059366285892 35.00662137573885 18.118344979059227 54-55 15.980663429549638 30.209623066073792 33.98057729783271 19.829136206543858 56-57 17.254605355239498 30.72641336764247 32.26144206027067 19.757539216847363 58-59 16.63149621558769 30.977810316426396 33.76336387420463 18.62732959378129 60-61 16.763116245518457 32.89262604838449 31.945984647021458 18.39827305907559 62-63 15.478677016827985 33.285602006869006 32.35645611050699 18.87926486579602 64-65 15.810284127001218 33.1324490477062 31.6706323144669 19.38663451082568 66-67 16.032159602930648 32.90195466217344 30.52659061912888 20.539295115767032 68-69 15.982321417516546 31.73477819677382 31.55013283161473 20.732767554094902 70-71 17.204541294775037 30.92074805396152 31.187218053207864 20.687492598055577 72-73 16.346992388109516 30.049741066525986 32.2792067268871 21.324059818477405 74-75 16.13435471194324 31.329604547754652 30.937705235731745 21.598335504570365 76-77 16.07325502524736 30.010174309062133 32.27005523196348 21.646515433727025 78-79 16.324113650800488 29.66322498681108 32.22806601996103 21.78459534242741 80-81 16.872441275506496 29.919816538949135 31.186225348094197 22.02151683745017 82-83 16.732971215391988 29.213120225668742 31.857675805748308 22.196232753190962 84-85 16.183144464482787 29.21509515221663 31.817986056902907 22.783774326397673 86-87 16.27364267430374 29.79750808164276 31.39445684093228 22.534392403121224 88-89 16.41778189295981 29.09583230154714 30.86153249857344 23.624853306919608 90-91 16.6864956528761 27.935721784070417 31.449489919517642 23.92829264353584 92-93 17.119486224308524 28.659521322983174 31.313993174061434 22.906999278646868 94-95 16.42724676534158 27.694382312804322 32.01120791983269 23.86716300202141 96-97 16.143237045251453 28.30988038457812 31.747343374855998 23.799539195314434 98-99 16.536257512993814 28.54439698215184 31.027150405223903 23.892195099630438 100-101 15.975710202038146 28.015364158551225 31.518327707704152 24.490597931706475 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3170.0 1 1814.0 2 536.5 3 671.0 4 501.0 5 363.0 6 658.0 7 1215.0 8 1630.0 9 1671.5 10 1235.5 11 760.0 12 669.5 13 624.5 14 570.0 15 563.5 16 568.0 17 594.0 18 642.5 19 672.0 20 712.0 21 780.5 22 862.0 23 947.0 24 1039.0 25 1178.0 26 1474.0 27 1779.5 28 1900.5 29 2231.0 30 2932.0 31 3484.0 32 4430.0 33 5615.0 34 6122.0 35 6957.0 36 8916.5 37 10118.0 38 9712.0 39 8919.5 40 8222.5 41 7299.0 42 6112.0 43 5931.0 44 6426.0 45 6537.5 46 6007.5 47 6322.5 48 7868.0 49 7477.0 50 5891.5 51 4395.0 52 2668.0 53 1554.0 54 1033.5 55 824.0 56 704.0 57 618.5 58 522.5 59 418.5 60 341.5 61 274.0 62 193.5 63 127.0 64 82.5 65 62.5 66 52.5 67 43.0 68 28.5 69 11.0 70 9.5 71 11.5 72 9.0 73 8.5 74 7.0 75 5.0 76 2.5 77 2.5 78 2.0 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009151494923611933 3 0.00807484846201053 4 0.0 5 5.38323230800702E-4 6 5.38323230800702E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0024224545386031587 26-27 0.01857215146262422 28-29 2.69161615400351E-4 30-31 0.0 32-33 0.003229939384804212 34-35 0.012919757539216849 36-37 0.006459878769608424 38-39 0.017495505001022812 40-41 0.020456282770426677 42-43 0.01614969692402106 44-45 0.007805686846610178 46-47 0.002153292923202808 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.38323230800702E-4 68-69 2.69161615400351E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.69161615400351E-4 82-83 0.001614969692402106 84-85 0.003768262615604914 86-87 8.07484846201053E-4 88-89 0.0 90-91 0.003768262615604914 92-93 0.0 94-95 2.69161615400351E-4 96-97 0.0 98-99 2.69161615400351E-4 100-101 0.00269161615400351 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 185762.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.575424467867485 #Duplication Level Percentage of deduplicated Percentage of total 1 78.21822258205144 27.044282468965662 2 9.119075792489257 6.305918325599423 3 3.4969172323597184 3.6272219291351298 4 1.9399638786821949 2.6830029823106987 5 1.2580183097714392 2.174825852434836 6 0.8563243445226382 1.7764666616423164 7 0.6461356417761724 1.563828985476039 8 0.4857694463473874 1.3436547840785522 9 0.4125926387245438 1.2839009054596742 >10 3.011147786012331 21.348822687094238 >50 0.3798966182973158 9.01906740883496 >100 0.15258142866039734 9.49440682163198 >500 0.009341720122065144 2.2211216502836963 >1k 0.014012580183097714 10.113478537052787 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3435 1.8491402978004112 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3072 1.6537289650197566 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3016 1.6235828640949173 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2247 1.2096122996091774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1948 1.0486536535997675 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1500 0.807484846201053 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1284 0.6912070283481013 TruSeq Adapter, Index 11 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1242 0.6685974526544718 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1043 0.5614711297251321 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 809 0.4355034937177679 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 734 0.39512925140771527 RNA PCR Primer, Index 11 (95% over 23bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 719 0.3870544029457047 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 674 0.3628298575596731 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 660 0.3552933323284633 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 530 0.285311312324372 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 468 0.25193527201472854 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 458 0.24655203970672152 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 438 0.23578557509070747 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 395 0.2126376761662773 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 394 0.21209935293547655 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 361 0.1943346863190534 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 358 0.1927197166266513 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 331 0.17818498939503236 TruSeq Adapter, Index 11 (96% over 28bp) GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 321 0.17280175708702533 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 317 0.17064846416382254 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 312 0.16795684800981903 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 309 0.1663418783174169 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 298 0.16042032277860918 TruSeq Adapter, Index 11 (96% over 26bp) CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 285 0.15342212077820008 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 282 0.15180715108579795 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 270 0.14534727231618952 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 264 0.14211733293138532 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 261 0.1405023632389832 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 257 0.13834907031578042 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 254 0.1367341006233783 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 249 0.13404248446937478 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 239 0.12865925216136775 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 217 0.11681614108375234 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 216 0.11627781785295162 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 213 0.11466284816054953 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 209 0.11250955523734671 TruSeq Adapter, Index 11 (95% over 24bp) CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 206 0.11089458554494462 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 200 0.10766464616014039 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 197 0.10604967646773827 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 194 0.10443470677533619 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 193 0.10389638354453548 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 192 0.10335806031373479 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 188 0.10120476739053197 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 187 0.10066644415973128 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 186 0.10012812092893056 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.003499101000204563 0.0 24-25 0.0 0.0 0.0 0.019917959539625975 0.0 26-27 0.0 0.0 0.0 0.05275567661846879 0.0 28-29 0.0 0.0 0.0 0.1405023632389832 0.0 30-31 0.0 0.0 0.0 0.28854125170917627 0.0 32-33 0.0 0.0 0.0 0.5364390994928996 0.0 34-35 0.0 0.0 0.0 0.9264542802080081 0.0 36-37 0.0 0.0 0.0 1.423864945467857 0.0 38-39 0.0 0.0 0.0 1.9710705095767702 0.0 40-41 0.0 0.0 0.0 2.4762868616832288 0.0 42-43 0.0 0.0 0.0 2.9559328603266546 0.0 44-45 0.0 0.0 0.0 3.502330939589367 0.0 46-47 0.0 0.0 0.0 4.07995176623852 0.0 48-49 0.0 0.0 0.0 4.592166320345388 0.0 50-51 0.0 0.0 0.0 5.0640066321422035 0.0 52-53 0.0 0.0 0.0 5.495203540013566 0.0 54-55 0.0 0.0 0.0 6.004457316351029 0.0 56-57 0.0 0.0 0.0 6.616530829771428 0.0 58-59 0.0 0.0 0.0 7.15512322218753 0.0 60-61 0.0 0.0 0.0 7.649303948062575 0.0 62-63 0.0 0.0 0.0 8.111992764935778 0.0 64-65 0.0 0.0 0.0 8.641433662428268 0.0 66-67 0.0 0.0 0.0 9.191061681075785 0.0 68-69 0.0 0.0 0.0 9.806903457111787 0.0 70-71 0.0 0.0 0.0 10.435664990687009 0.0 72-73 0.0 0.0 0.0 10.999020251719944 0.0 74-75 0.0 0.0 0.0 11.643393158988383 0.0 76-77 0.0 0.0 0.0 12.2716163693328 0.0 78-79 0.0 0.0 0.0 12.985163811759133 0.0 80-81 0.0 0.0 0.0 13.701402870339468 0.0 82-83 0.0 0.0 0.0 14.352504817992916 0.0 84-85 0.0 0.0 0.0 15.024063048416792 0.0 86-87 0.0 0.0 0.0 15.702888642456477 0.0 88-89 0.0 0.0 0.0 16.41858937780601 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCAACG 30 1.2942194E-4 63.374283 2 GAGTGAG 25 0.005017875 56.990795 9 CGCAGAA 85 1.2732926E-10 50.285995 9 CGTCTTT 20 5.3877325E-4 47.49233 72-73 GGAGGTA 40 5.3807336E-4 47.49233 8 GTAGATA 55 4.9519054E-5 43.174843 6 ATGGGAC 60 8.271787E-5 39.576942 5 GATGACC 60 8.271787E-5 39.576942 9 AGATATA 65 1.32516E-4 36.532562 8 CATGTAG 65 1.32516E-4 36.532562 3 TGTAGAT 65 1.32516E-4 36.532562 5 GAGATGT 60 0.003930041 31.687141 1 GGATGAC 75 3.0715027E-4 31.661552 8 AGATGTG 80 4.4623722E-4 29.706694 2 TATGGGA 65 0.0058362354 29.22605 4 ATGTCTC 45 8.9445355E-4 26.384628 84-85 ACCCTTG 130 9.974685E-6 25.572794 7 ATGGGAT 170 1.1140037E-7 25.142998 5 ATATAGG 95 0.0012180842 25.016163 2 CACCATA 95 0.001223838 24.995962 7 >>END_MODULE