##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938057_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 189625 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.29471852340145 33.0 31.0 34.0 30.0 34.0 2 31.47150428477258 33.0 31.0 34.0 30.0 34.0 3 31.485263019116676 34.0 31.0 34.0 28.0 34.0 4 34.94682135794331 37.0 35.0 37.0 33.0 37.0 5 34.97654317732366 37.0 35.0 37.0 33.0 37.0 6 35.04844825313118 37.0 35.0 37.0 33.0 37.0 7 35.01263546473302 37.0 35.0 37.0 33.0 37.0 8 35.00890177982861 37.0 35.0 37.0 33.0 37.0 9 36.600606460118655 39.0 37.0 39.0 33.0 39.0 10-11 36.55033882663151 39.0 37.0 39.0 33.0 39.0 12-13 36.484529993408046 39.0 37.0 39.0 33.0 39.0 14-15 37.60817666446935 40.0 38.0 41.0 33.0 41.0 16-17 37.53588398154252 40.0 38.0 41.0 33.0 41.0 18-19 37.514162162162165 40.0 38.0 41.0 33.0 41.0 20-21 37.44814502307185 40.0 38.0 41.0 33.0 41.0 22-23 37.29796704021094 40.0 37.5 41.0 32.5 41.0 24-25 37.193745550428474 40.0 37.0 41.0 32.5 41.0 26-27 37.09362162162162 40.0 37.0 41.0 32.0 41.0 28-29 36.94490969017798 40.0 37.0 41.0 32.0 41.0 30-31 36.66529993408042 40.0 36.0 41.0 32.0 41.0 32-33 36.26064073829927 39.5 35.0 41.0 31.0 41.0 34-35 35.998549769281475 39.0 35.0 41.0 30.0 41.0 36-37 35.7451048121292 39.0 35.0 41.0 30.0 41.0 38-39 35.467411997363214 39.0 35.0 41.0 29.5 41.0 40-41 35.21111931443639 39.0 35.0 41.0 27.0 41.0 42-43 34.81168622280818 38.5 35.0 41.0 24.5 41.0 44-45 34.35626631509558 38.5 35.0 41.0 18.0 41.0 46-47 33.922204350692155 39.0 35.0 41.0 5.5 41.0 48-49 33.506433750823994 39.0 35.0 41.0 2.0 41.0 50-51 32.793112722478575 38.0 34.0 40.0 2.0 40.5 52-53 32.815037574159525 38.0 34.0 40.0 2.0 41.0 54-55 32.906172709294665 39.0 34.5 41.0 2.0 41.0 56-57 32.652100197758735 38.5 34.0 41.0 2.0 41.0 58-59 32.38531575477917 38.0 34.0 41.0 2.0 41.0 60-61 31.99586816084377 38.0 33.0 40.0 2.0 41.0 62-63 31.607177323665127 38.0 32.5 40.0 2.0 41.0 64-65 31.199272247857614 37.0 32.0 40.0 2.0 41.0 66-67 30.760208305866843 36.5 31.0 40.0 2.0 41.0 68-69 30.263066578773895 36.0 30.5 39.0 2.0 41.0 70-71 29.75713909030982 35.5 29.5 39.0 2.0 40.5 72-73 29.267688859591296 35.0 29.0 38.0 2.0 40.0 74-75 28.76546605141727 35.0 28.5 37.0 2.0 39.5 76-77 28.282109426499673 35.0 27.5 37.0 2.0 39.0 78-79 27.72788661832564 34.5 26.0 36.0 2.0 39.0 80-81 27.21728147659855 34.0 25.5 36.0 2.0 37.5 82-83 26.719100856954515 34.0 24.0 35.0 2.0 37.0 84-85 26.255757415952537 34.0 21.0 35.0 2.0 36.5 86-87 25.83799868160844 34.0 19.0 35.0 2.0 36.0 88-89 25.327646671061306 33.0 13.5 35.0 2.0 36.0 90-91 24.772727752142387 33.0 4.5 35.0 2.0 35.0 92-93 24.22721951219512 32.0 2.0 35.0 2.0 35.0 94-95 23.7732867501648 32.0 2.0 35.0 2.0 35.0 96-97 23.11979433091628 31.5 2.0 35.0 2.0 35.0 98-99 22.2929650626236 31.0 2.0 34.0 2.0 35.0 100-101 19.89818325642716 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.17482007217370565 1101 2 -0.16062877838359313 1101 3 0.15011963741314105 1101 4 -0.006975950683568044 1101 5 -0.6424449203243867 1101 6 -0.9079863556510119 1101 7 -0.6168513568358591 1101 8 -0.3489004808397027 1101 9 -0.56674986269757 1101 10-11 -0.48334849531688917 1101 12-13 -0.5677772536698455 1101 14-15 -0.35761552951542797 1101 16-17 -0.08723797661449595 1101 18-19 0.07231974241552308 1101 20-21 -0.07679431216372024 1101 22-23 -0.06353553413163127 1101 24-25 0.10389647993326179 1101 26-27 -0.25279475493098147 1101 28-29 0.002526661460528601 1101 30-31 -0.059338211188986634 1101 32-33 0.1427713489922695 1101 34-35 0.28737069939931814 1101 36-37 0.6275638533900363 1101 38-39 0.6893492083769033 1101 40-41 1.0731610854406526 1101 42-43 0.8730636995477639 1101 44-45 0.8191369687420504 1101 46-47 1.3955487835403204 1101 48-49 1.6759386164520507 1101 50-51 1.4230338806302854 1101 52-53 1.6593783226463117 1101 54-55 2.0721317285740426 1101 56-57 1.9580400464521404 1101 58-59 2.032344827646398 1101 60-61 1.8722798426992 1101 62-63 1.9164595238327635 1101 64-65 1.7118872136136574 1101 66-67 1.3832470651552882 1101 68-69 1.4511292744201256 1101 70-71 1.4381920253158853 1101 72-73 1.0929444826514398 1101 74-75 1.0078010582863541 1101 76-77 1.2536962405111218 1101 78-79 0.9056132368397698 1101 80-81 0.9797673894606511 1101 82-83 0.7936367421940496 1101 84-85 0.7237092736447899 1101 86-87 1.030222553324716 1101 88-89 1.2521373966580676 1101 90-91 0.7667821046057348 1101 92-93 0.34108233367370744 1101 94-95 -0.17199672444168712 1101 96-97 0.04548672993745484 1101 98-99 0.04140631976817488 1101 100-101 0.7399901624763672 1102 1 1.1226421080460582 1102 2 1.1607082819471763 1102 3 1.1254282793884478 1102 4 1.1878761341056219 1102 5 1.2763764167359426 1102 6 1.164457157044069 1102 7 1.1837542802477614 1102 8 1.2619776929315591 1102 9 0.893650789526987 1102 10-11 1.2513941089225682 1102 12-13 1.2583932564629308 1102 14-15 1.5727362058677485 1102 16-17 1.6551915598401976 1102 18-19 1.829657268620977 1102 20-21 1.855886796618016 1102 22-23 1.3698443866696763 1102 24-25 1.479216768775565 1102 26-27 1.4939441223110421 1102 28-29 1.4992655387025522 1102 30-31 1.7108691030434855 1102 32-33 1.6643762872638774 1102 34-35 1.7784608461498408 1102 36-37 2.1269815100403875 1102 38-39 2.2065283371912585 1102 40-41 2.589816105240331 1102 42-43 2.9004338369807954 1102 44-45 2.3989180076425853 1102 46-47 2.693184469540796 1102 48-49 2.379176445476041 1102 50-51 2.969796051606288 1102 52-53 3.204044057563621 1102 54-55 3.6545333125479544 1102 56-57 3.4467425854691385 1102 58-59 3.595936721426966 1102 60-61 3.6673532179612565 1102 62-63 3.6107874996072837 1102 64-65 3.652604660620412 1102 66-67 3.4629698697208653 1102 68-69 3.871872344534264 1102 70-71 3.896088601136995 1102 72-73 3.7048708744520518 1102 74-75 3.7183764135157986 1102 76-77 3.601821094925281 1102 78-79 3.4663516482071124 1102 80-81 2.826844025845464 1102 82-83 2.763587825352676 1102 84-85 2.337499164164239 1102 86-87 2.5684242770610304 1102 88-89 2.571727426939926 1102 90-91 2.4974483053977217 1102 92-93 1.85203038864692 1102 94-95 2.178748675045849 1102 96-97 1.8964068324298857 1102 98-99 2.144539326989232 1102 100-101 2.2574022286305713 1103 1 0.43373448006390447 1103 2 0.5198664281823788 1103 3 0.6933984842301619 1103 4 0.5299231458058458 1103 5 0.753134085124266 1103 6 0.4275540264443052 1103 7 0.6493739000784089 1103 8 0.9579827524867355 1103 9 1.0748461094384396 1103 10-11 0.6432636848334639 1103 12-13 0.5176251878778473 1103 14-15 0.6326251767401772 1103 16-17 0.9085031089776194 1103 18-19 0.6485146524093253 1103 20-21 0.48272703413602613 1103 22-23 0.052989743473716544 1103 24-25 0.10577591989463286 1103 26-27 0.21380638997556645 1103 28-29 0.3075821825309504 1103 30-31 0.48466482350305995 1103 32-33 0.42135952748733985 1103 34-35 0.635756794992119 1103 36-37 0.4880369894683838 1103 38-39 0.3812762378272154 1103 40-41 0.11223274555514706 1103 42-43 0.005314199582251433 1103 44-45 0.29185922544941434 1103 46-47 0.920671205776344 1103 48-49 1.189249879297762 1103 50-51 1.1252719660154824 1103 52-53 1.0332489344126472 1103 54-55 1.3985163068208166 1103 56-57 1.1731928156086298 1103 58-59 1.1265927068104773 1103 60-61 1.3637447713149626 1103 62-63 1.2027378878578716 1103 64-65 0.8212740678794717 1103 66-67 0.9009551829565225 1103 68-69 1.0707409717785659 1103 70-71 1.331684679981727 1103 72-73 1.632912756325485 1103 74-75 1.3677914024480202 1103 76-77 0.9492614519050093 1103 78-79 0.5607791103482889 1103 80-81 0.4441097578998523 1103 82-83 0.15531685806410778 1103 84-85 0.5392137911977208 1103 86-87 0.6247118284471362 1103 88-89 0.9041823652765935 1103 90-91 1.0684753248278192 1103 92-93 1.2468689396465322 1103 94-95 0.7831898401489745 1103 96-97 0.5389000687884113 1103 98-99 0.38448170427745154 1103 100-101 0.6951767270670288 1104 1 0.4945592870440052 1104 2 0.3515781851342332 1104 3 0.5205593655307688 1104 4 0.7688598101709161 1104 5 0.6731328297102905 1104 6 0.4416460482885043 1104 7 0.1476790912151884 1104 8 0.30077353774817794 1104 9 0.21366350947610613 1104 10-11 0.3777279369205928 1104 12-13 0.5066089302668999 1104 14-15 0.6595851918051352 1104 16-17 0.7287905987773726 1104 18-19 0.7368016400434882 1104 20-21 0.4686350122918199 1104 22-23 0.4120852177063483 1104 24-25 0.7737189241002724 1104 26-27 0.9153631033017788 1104 28-29 0.8233062425397364 1104 30-31 0.685217205651071 1104 32-33 0.5716796580503924 1104 34-35 0.9150236332320247 1104 36-37 1.3213493992355083 1104 38-39 0.7826006995684693 1104 40-41 1.0190967214511915 1104 42-43 1.1059670148480834 1104 44-45 0.9783823564518599 1104 46-47 1.6171121071635781 1104 48-49 2.075681992787189 1104 50-51 1.4652380698382217 1104 52-53 1.580483885137646 1104 54-55 1.7432372910653768 1104 56-57 1.22909012274566 1104 58-59 1.7509257681374493 1104 60-61 1.7961250362073216 1104 62-63 1.907338980068026 1104 64-65 2.0804542825552588 1104 66-67 1.815241793966507 1104 68-69 1.5759038617415264 1104 70-71 1.422482495561411 1104 72-73 1.1383738071069835 1104 74-75 1.208730452099644 1104 76-77 1.1027044247252995 1104 78-79 1.3325448501009696 1104 80-81 1.4227206423389944 1104 82-83 1.632484016434578 1104 84-85 1.2967776603835866 1104 86-87 1.351973142865564 1104 88-89 1.422480023929534 1104 90-91 0.9553311405330476 1104 92-93 0.9349927234642479 1104 94-95 1.2637780015952096 1104 96-97 1.3118898371645322 1104 98-99 0.6043831542805904 1104 100-101 0.11115814694023385 1105 1 0.14496231586667463 1105 2 0.0023476037956875473 1105 3 0.14354422141743584 1105 4 0.34158819317043054 1105 5 -0.06642505988423153 1105 6 -0.03734760095429124 1105 7 0.1249629212102974 1105 8 0.26730993247216617 1105 9 0.11534890273453158 1105 10-11 0.27275724810769475 1105 12-13 -0.010611392156157251 1105 14-15 0.2168946690889726 1105 16-17 0.06309079418529251 1105 18-19 0.349191723091856 1105 20-21 0.4883895310998341 1105 22-23 0.1502996778919865 1105 24-25 0.36137769303041267 1105 26-27 0.3258418526911342 1105 28-29 0.41087341401018307 1105 30-31 0.5227538446349485 1105 32-33 0.4469849829160495 1105 34-35 0.49691176220125044 1105 36-37 0.04973239874698265 1105 38-39 0.5351959609265649 1105 40-41 0.15783634040576544 1105 42-43 0.46683047356815877 1105 44-45 0.6858506026658304 1105 46-47 0.12266901971852917 1105 48-49 0.15705106948478686 1105 50-51 0.22271852904682277 1105 52-53 0.6770581401449718 1105 54-55 1.0517680678118282 1105 56-57 0.7560808408697426 1105 58-59 0.905633877565883 1105 60-61 1.04679675091176 1105 62-63 0.8761347787978764 1105 64-65 0.567630638197663 1105 66-67 0.5546775536147663 1105 68-69 0.3800074279359613 1105 70-71 0.6424995937538895 1105 72-73 0.2722587606975999 1105 74-75 0.13172752098093454 1105 76-77 0.5168470725025323 1105 78-79 0.35495569524019643 1105 80-81 0.33289406686269274 1105 82-83 0.33641049420693037 1105 84-85 0.8368668152443561 1105 86-87 1.1042630793579953 1105 88-89 0.8618730380966078 1105 90-91 0.6809662162536156 1105 92-93 0.4727919418325932 1105 94-95 0.8173014505448926 1105 96-97 0.7456343413169506 1105 98-99 0.38604534284922565 1105 100-101 0.8026579026803375 1106 1 0.4384977849742242 1106 2 0.49096316443641896 1106 3 0.6113931916691548 1106 4 0.3044600467173382 1106 5 0.3775680751272077 1106 6 0.3770688750182529 1106 7 0.6299972461530885 1106 8 0.3570124496346381 1106 9 0.20219100799769052 1106 10-11 -0.054531081411759885 1106 12-13 0.19957167420539434 1106 14-15 0.1390056530706616 1106 16-17 0.40522466146217795 1106 18-19 0.4176413798424292 1106 20-21 0.22005429082523165 1106 22-23 -0.16337309098673103 1106 24-25 -0.5801840918666059 1106 26-27 -0.06592016103883935 1106 28-29 -0.1544583937701347 1106 30-31 0.23897238010405886 1106 32-33 0.5091131293691404 1106 34-35 -0.12374041171179329 1106 36-37 -0.4520982648685745 1106 38-39 -0.6702674258011285 1106 40-41 -0.049028648152585674 1106 42-43 -0.15036060194670853 1106 44-45 0.26442611753997625 1106 46-47 0.5752433904279144 1106 48-49 -0.10092947971428856 1106 50-51 -0.30974714601038755 1106 52-53 -0.24299334726921273 1106 54-55 -0.9276083623941176 1106 56-57 -0.7081068021508514 1106 58-59 -0.26258694623274437 1106 60-61 -0.4078371163651262 1106 62-63 0.23374347445005128 1106 64-65 0.16877480295738678 1106 66-67 -0.13679841892527733 1106 68-69 -0.5508850205766258 1106 70-71 -0.5092738616236865 1106 72-73 -0.12599638804381286 1106 74-75 0.06748266972235228 1106 76-77 0.4600965004201534 1106 78-79 0.46016164489694944 1106 80-81 -0.5986276722677459 1106 82-83 -0.30412154240634237 1106 84-85 -0.7624180817808153 1106 86-87 -0.43569687487541486 1106 88-89 -0.6922745591573936 1106 90-91 -0.7787019759660652 1106 92-93 -0.5470593167486477 1106 94-95 -0.7509765814917202 1106 96-97 -0.9401208074244671 1106 98-99 -0.7000816594390997 1106 100-101 -0.9521475893105062 1107 1 0.2786360093229767 1107 2 0.47517369075221083 1107 3 0.30724646120663124 1107 4 0.5244494140751286 1107 5 0.5691151245689952 1107 6 0.16664888565089342 1107 7 0.1328680595502192 1107 8 0.2341522688797184 1107 9 0.2693893067749329 1107 10-11 0.40357630827458024 1107 12-13 0.279024093131504 1107 14-15 0.40697481840824423 1107 16-17 0.5513703286604752 1107 18-19 0.33747125756703866 1107 20-21 0.43302611432336846 1107 22-23 0.33096502703559594 1107 24-25 0.41899187836199303 1107 26-27 0.3897417209388223 1107 28-29 0.3849621272293362 1107 30-31 0.02876504358093257 1107 32-33 -0.17400755111995636 1107 34-35 0.00454241657103438 1107 36-37 0.2594381519310005 1107 38-39 0.08946144182247195 1107 40-41 -0.0646054689925677 1107 42-43 0.0411332842840153 1107 44-45 -0.48754092126917215 1107 46-47 -0.624092069433857 1107 48-49 -0.5779895541427891 1107 50-51 -0.2447551204920515 1107 52-53 0.004215584150237817 1107 54-55 0.21242353553251547 1107 56-57 -0.1729925012470801 1107 58-59 -0.06529826999670263 1107 60-61 -0.3019625815432221 1107 62-63 -0.10009490938833565 1107 64-65 -0.06532939693628492 1107 66-67 0.17056468580624795 1107 68-69 0.2754839321081164 1107 70-71 0.4499765371003974 1107 72-73 0.07498713136075352 1107 74-75 0.3018526856713173 1107 76-77 0.49752340100494763 1107 78-79 0.040012787905990166 1107 80-81 0.26399859035851847 1107 82-83 0.6665323650896688 1107 84-85 0.6423400256088705 1107 86-87 0.5156322054010332 1107 88-89 0.1901284179824252 1107 90-91 0.6955138666708294 1107 92-93 0.2759869352449762 1107 94-95 -0.20342208920038374 1107 96-97 0.009852610970003894 1107 98-99 0.25212461381980233 1107 100-101 0.11260520175807187 1108 1 0.457665106262521 1108 2 0.45527319323977267 1108 3 0.12542827938845136 1108 4 0.2230774858375284 1108 5 0.22221327020444903 1108 6 0.4763880685244075 1108 7 0.48836331056786264 1108 8 0.27953143366186595 1108 9 0.49470213656537965 1108 10-11 0.2568855197927462 1108 12-13 0.48349416700455805 1108 14-15 0.32379224641970694 1108 16-17 0.3923548002419679 1108 18-19 0.4508437888500225 1108 20-21 0.7622512010111464 1108 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2316 56-57 -1.8623019133598362 2316 58-59 -2.3645248453739036 2316 60-61 -2.1166787811966614 2316 62-63 -1.795754433499745 2316 64-65 -1.6349798766286057 2316 66-67 -1.1642373090375884 2316 68-69 -1.6495086717547807 2316 70-71 -1.5750963676796204 2316 72-73 -1.464677295332958 2316 74-75 -1.7775184313567038 2316 76-77 -2.030951726842563 2316 78-79 -1.9599660802737198 2316 80-81 -1.953648592769838 2316 82-83 -2.1653745703737535 2316 84-85 -1.8531800284885875 2316 86-87 -2.032823058535403 2316 88-89 -2.1477799544447755 2316 90-91 -1.6956255610794742 2316 92-93 -1.7789135338814326 2316 94-95 -2.0439296755238736 2316 96-97 -2.013529748186418 2316 98-99 -1.9148801619715847 2316 100-101 -2.069181282329666 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4209.0 3 572.0 4 459.0 5 365.0 6 343.0 7 348.0 8 400.0 9 434.0 10 551.0 11 716.0 12 1068.0 13 1378.0 14 1627.0 15 2171.0 16 2865.0 17 3917.0 18 3162.0 19 2109.0 20 2078.0 21 2017.0 22 2056.0 23 1888.0 24 1995.0 25 2124.0 26 2100.0 27 2317.0 28 2600.0 29 3000.0 30 3458.0 31 4258.0 32 5150.0 33 6814.0 34 9650.0 35 17414.0 36 20581.0 37 33336.0 38 35155.0 39 4940.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.68137699252026 24.131295621888967 16.301161453115142 17.886165932475627 2 15.071492639687648 25.960006331451485 38.417664749643855 20.55083627921701 3 18.567765219868935 31.110900053819606 32.17621173267484 18.145122993636623 4 11.472537678895597 22.278570523916073 39.15755477688184 27.0913370203065 5 9.812418190263058 41.29861081788625 35.66535067347887 13.223620318371829 6 24.2811889551766 41.80930257114196 17.609946676521833 16.2995617971596 7 21.049992875500696 38.73933304131682 24.56263490371371 15.648039179468775 8 23.165603516047504 35.031736233122814 21.962106462795006 19.840553788034676 9 24.07097669461343 20.088430672146977 24.17439203912899 31.6662005941106 10-11 21.671025166709327 30.587962095563302 27.618200673962207 20.122812063765164 12-13 24.509055967223762 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17.391292875293225 38-39 16.12430952343268 31.952643991073266 35.16167835266932 16.761368132824742 40-41 15.893838832554263 31.933819417332305 35.14761899737262 17.024722752740818 42-43 16.106592945682653 31.585625463284156 33.806223901109774 18.50155768992342 44-45 16.464125406796736 31.039448075066854 34.317820993612564 18.178605524523846 46-47 17.40219802246551 31.336194050517065 34.14504205359757 17.11656587341986 48-49 16.79172677904054 31.36111997594626 33.9387209845154 17.908432260497797 50-51 15.766098404928686 31.264241708161027 34.59126297577855 18.37839691113174 52-53 16.48173985056401 31.86183105240809 33.39891707700463 18.257512020023263 54-55 16.752514103507483 30.83465862786521 33.42379622376774 18.989031044859573 56-57 17.44195246484445 30.744358760667982 32.62529933644889 19.188389438038676 58-59 17.535966242500166 30.010681083932223 33.37456319641326 19.07878947715435 60-61 17.24976460690012 31.06054811645958 33.004090696895446 18.685596579744853 62-63 16.66055723308588 30.796028428644895 33.56610714803729 18.977307190231937 64-65 16.962964721147532 30.6852436448221 32.988904976277695 19.36288665775267 66-67 17.134403299560656 30.513552143712324 31.862701146091 20.48934341063602 68-69 16.821648592767627 29.599350183552048 33.21527279632052 20.363728427359803 70-71 17.857350124775113 29.130109052922588 32.84882954083813 20.16371128146417 72-73 16.967823283934855 28.69209219876864 33.63123657984563 20.70884793745087 74-75 16.76531565037112 30.094534213261166 32.44417364331271 20.695976493055007 76-77 16.95238446890775 28.805101777076818 33.22089238900523 21.02162136501021 78-79 16.979733917010588 28.54946532877995 33.498503448003056 20.972297306206407 80-81 17.930183310345917 29.01873188587342 32.10475931781441 20.94632548596625 82-83 17.741301693377604 28.25522241239718 32.89862678442862 21.10484910979659 84-85 17.13318248706467 28.122544133082272 32.93195567440413 21.812317705448926 86-87 17.477236108950237 28.49092476906484 32.57537201458788 21.456467107397046 88-89 17.855503558596823 27.947251518141194 32.34366179349059 21.853583129771394 90-91 18.290874745406768 26.92039806245317 32.5494148313089 22.23931236083116 92-93 19.055469535146884 27.598395184498393 32.13930558229955 21.206829698055177 94-95 18.293635298711443 26.49329337146596 32.92052302204587 22.292548307776734 96-97 18.075194158365694 26.596002870167148 32.799837497889584 22.52896547357758 98-99 18.836798915351523 26.780160850837092 32.13508516020458 22.2479550736068 100-101 18.6041842772612 25.878064243448858 32.73378064243449 22.783970836855453 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3495.0 1 1966.0 2 538.5 3 659.0 4 505.0 5 413.5 6 727.0 7 1254.5 8 1628.0 9 1667.0 10 1259.0 11 795.5 12 689.0 13 688.0 14 676.5 15 641.5 16 642.0 17 668.5 18 705.0 19 772.5 20 878.5 21 929.5 22 998.0 23 1133.5 24 1223.0 25 1356.5 26 1608.0 27 1889.5 28 2067.5 29 2380.0 30 3050.5 31 3575.5 32 4369.5 33 5535.5 34 6067.5 35 6945.0 36 9035.5 37 10275.0 38 9861.5 39 9039.0 40 8228.0 41 7264.0 42 5983.5 43 5574.5 44 5940.5 45 5909.0 46 5420.0 47 5858.5 48 7688.5 49 7559.5 50 5448.5 51 3707.5 52 2606.0 53 1905.0 54 1372.0 55 1100.5 56 941.0 57 843.0 58 742.5 59 661.0 60 597.0 61 516.0 62 447.5 63 377.0 64 304.0 65 254.0 66 226.5 67 211.0 68 182.0 69 157.5 70 142.5 71 114.0 72 90.0 73 83.5 74 73.0 75 55.0 76 46.5 77 34.5 78 22.5 79 20.0 80 17.5 81 10.0 82 5.5 83 5.0 84 3.0 85 2.0 86 3.5 87 3.0 88 2.5 89 2.0 90 1.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5178642056690838 2 0.050098879367172056 3 0.05431773236651285 4 0.06802900461437048 5 0.08490441661173369 6 0.1133816743572841 7 0.07277521423862887 8 0.04957152274225445 9 0.05168094924192485 10-11 0.07831245880026368 12-13 0.1157547791694133 14-15 0.054581410678971654 16-17 0.06143704680290046 18-19 0.06170072511535926 20-21 0.07883981542518127 22-23 0.06750164798945286 24-25 0.0959789057350033 26-27 0.07198417930125248 28-29 0.07092946605141727 30-31 0.07699406723796967 32-33 0.07567567567567568 34-35 0.07936717205009887 36-37 0.07198417930125248 38-39 0.043243243243243246 40-41 0.04377059986816084 42-43 0.04297956493078444 44-45 0.01793012524719842 46-47 0.023994726433750824 48-49 0.026104152933421224 50-51 0.021621621621621623 52-53 0.023994726433750824 54-55 0.02346736980883322 56-57 0.02056690837178642 58-59 0.01977587343441002 60-61 0.025576796308503624 62-63 0.014502307185234015 64-65 0.018721160184574818 66-67 0.012656558998022413 68-69 0.017402768622280816 70-71 0.04429795649307844 72-73 0.04113381674357284 74-75 0.03375082399472643 76-77 0.023203691496374423 78-79 0.011865524060646013 80-81 0.015029663810151615 82-83 0.017666446934739618 84-85 0.014765985497692815 86-87 0.008174027686222808 88-89 0.04429795649307844 90-91 0.056427158866183255 92-93 0.03717864205669084 94-95 0.058536585365853655 96-97 0.04693473961766644 98-99 0.03717864205669084 100-101 0.18193803559657218 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 189625.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 38.56875411997363 #Duplication Level Percentage of deduplicated Percentage of total 1 80.75229709035222 31.14515491100857 2 8.00973528768322 6.178510217534608 3 3.1311529205863047 3.622940013183916 4 1.6886348720192519 2.6051417270929464 5 1.1047910741632028 2.130520764667106 6 0.7656967840735069 1.771918259723138 7 0.583843797856049 1.5762689518787079 8 0.4894990155327062 1.510349373764008 9 0.3336250273463137 1.1580751483190508 >10 2.6854080070006563 21.36479894528675 >50 0.31174797637278495 8.229400131839157 >100 0.12716035878363596 8.669215557020435 >500 0.00820389411507329 2.2897824653922214 >1k 0.00820389411507329 7.747923533289387 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3817 2.0129202373104813 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3132 1.6516809492419249 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2943 1.5520105471324983 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1928 1.0167435728411338 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1583 0.8348055372445616 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1289 0.6797626895187872 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 983 0.5183915622940013 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 767 0.4044825313117996 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 684 0.3607119314436388 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 681 0.35912986156888593 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 642 0.3385629531970995 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 585 0.3085036255767963 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 491 0.2589321028345419 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 491 0.2589321028345419 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 438 0.23098220171390904 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 434 0.2288727752142386 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 421 0.2220171390903098 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 403 0.21252471984179302 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 399 0.21041529334212258 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 347 0.18299274884640737 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 304 0.16031641397495056 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 301 0.15873434410019774 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 299 0.15767963085036255 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 281 0.14818721160184573 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 262 0.13816743572841134 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 253 0.13342122610415294 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 251 0.13236651285431772 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 244 0.12867501647989452 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 242 0.12762030323005932 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 234 0.12340145023071852 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 223 0.11760052735662492 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 222 0.11707317073170731 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 221 0.11654581410678973 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 210 0.11074489123269612 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 203 0.10705339485827291 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 201 0.10599868160843771 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 197 0.10388925510876731 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 192 0.1012524719841793 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 190 0.10019775873434411 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 190 0.10019775873434411 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.6367831245880025E-4 0.0 18-19 0.0 0.0 0.0 5.273566249176005E-4 0.0 20-21 0.0 0.0 0.0 0.0023731048121292024 0.0 22-23 0.0 0.0 0.0 0.007646671061305208 0.0 24-25 0.0 0.0 0.0 0.02109426499670402 0.0 26-27 0.0 0.0 0.0 0.04851680949241925 0.0 28-29 0.0 0.0 0.0 0.12366512854317732 0.0 30-31 0.0 0.0 0.0 0.2887277521423863 0.0 32-33 0.0 0.0 0.0 0.5239288068556361 0.0 34-35 0.0 0.0 0.0 0.9015161502966381 0.0 36-37 0.0 0.0 0.0 1.4061964403427818 0.0 38-39 0.0 0.0 0.0 1.9562294001318392 0.0 40-41 0.0 0.0 0.0 2.4340145023071855 0.0 42-43 0.0 0.0 0.0 2.9220830586684245 0.0 44-45 0.0 0.0 0.0 3.459723137771918 0.0 46-47 0.0 0.0 0.0 4.0711931443638765 0.0 48-49 0.0 0.0 0.0 4.5753460777851025 0.0 50-51 0.0 0.0 0.0 5.071324983520105 0.0 52-53 0.0 0.0 0.0 5.52748846407383 0.0 54-55 0.0 0.0 0.0 6.022148978246539 0.0 56-57 0.0 0.0 0.0 6.604878048780488 0.0 58-59 0.0 0.0 0.0 7.154911008569545 0.0 60-61 0.0 0.0 0.0 7.67013843111404 0.0 62-63 0.0 0.0 0.0 8.149241924851681 0.0 64-65 0.0 0.0 0.0 8.641265655899803 0.0 66-67 0.0 0.0 0.0 9.215029663810153 0.0 68-69 0.0 0.0 0.0 9.808305866842453 0.0 70-71 0.0 0.0 0.0 10.422940013183915 0.0 72-73 0.0 0.0 0.0 10.974555042847726 0.0 74-75 0.0 0.0 0.0 11.58048780487805 0.0 76-77 0.0 0.0 0.0 12.204614370468029 0.0 78-79 0.0 0.0 0.0 12.941067897165457 0.0 80-81 0.0 0.0 0.0 13.67514831905076 0.0 82-83 0.0 0.0 0.0 14.340936058009229 0.0 84-85 0.0 0.0 0.0 15.034410019775873 0.0 86-87 0.0 0.0 0.0 15.667237969676993 0.0 88-89 0.0 0.0 0.0 16.348846407382993 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGACA 20 0.0020569102 71.37612 9 GTATGGT 30 1.2720248E-4 63.59678 1 GTCCTAT 25 0.0049330676 57.237103 1 GACCCTC 25 0.0049798265 57.1009 7 CGCAGAA 115 0.0 49.652958 9 GTACCCA 60 8.064716E-5 39.747986 1 TAAGATC 50 0.001610958 38.006966 2 GTATTAT 90 5.656402E-7 36.95122 3 GTATAGT 80 1.0427526E-5 35.77319 1 ATGTATT 90 2.3351575E-5 31.798391 1 GATGACC 90 2.3735282E-5 31.722723 9 ACCCATG 60 0.0039391606 31.672474 3 AGATCCT 75 3.065521E-4 31.672472 4 GATCCTC 75 3.065521E-4 31.672472 5 GGATGAC 110 2.6803464E-6 30.280779 8 CTAGAGT 100 4.925432E-5 28.505226 3 ATTAAAT 85 6.3804403E-4 27.946302 3 TATAGTA 85 6.3804403E-4 27.946302 2 GTATATA 70 0.008206247 27.255764 1 TCCTCAT 105 6.7935835E-5 27.190905 7 >>END_MODULE