##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938057_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 189625 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39126170072512 33.0 31.0 34.0 31.0 34.0 2 32.606433750823996 34.0 31.0 34.0 31.0 34.0 3 32.620825313118 34.0 31.0 34.0 31.0 34.0 4 36.124904416611734 37.0 35.0 37.0 35.0 37.0 5 36.098220171390906 37.0 35.0 37.0 35.0 37.0 6 36.17017270929466 37.0 36.0 37.0 35.0 37.0 7 36.1401713909031 37.0 36.0 37.0 35.0 37.0 8 36.15756361239288 37.0 37.0 37.0 35.0 37.0 9 37.81822544495715 39.0 38.0 39.0 35.0 39.0 10-11 37.81963085036256 39.0 38.0 39.0 35.0 39.0 12-13 37.78826631509558 39.0 38.0 39.0 35.0 39.0 14-15 38.96564535266974 40.0 38.0 41.0 35.5 41.0 16-17 38.92025049439684 40.0 38.0 41.0 35.5 41.0 18-19 38.9838391562294 40.0 38.0 41.0 35.5 41.0 20-21 38.9724825313118 40.0 38.0 41.0 35.0 41.0 22-23 38.807815425181275 40.0 38.0 41.0 35.0 41.0 24-25 38.71945418589321 40.0 38.0 41.0 35.0 41.0 26-27 38.56827422544496 40.0 38.0 41.0 35.0 41.0 28-29 38.436437705998685 40.0 38.0 41.0 34.5 41.0 30-31 38.12731443638761 40.0 37.0 41.0 34.0 41.0 32-33 37.768669742913644 40.0 35.5 41.0 33.5 41.0 34-35 37.45450230718524 40.0 35.0 41.0 33.0 41.0 36-37 37.239720500988795 40.0 35.0 41.0 33.0 41.0 38-39 37.030323005932765 40.0 35.0 41.0 33.0 41.0 40-41 36.93614765985498 40.0 35.0 41.0 33.0 41.0 42-43 36.67033355306526 40.0 35.0 41.0 32.5 41.0 44-45 36.3043111404087 40.0 35.0 41.0 31.0 41.0 46-47 35.77499274884641 40.0 35.0 41.0 29.0 41.0 48-49 35.26608305866842 39.5 35.0 41.0 25.5 41.0 50-51 34.86044825313118 39.0 35.0 41.0 23.0 41.0 52-53 34.47203164139749 39.0 35.0 41.0 19.5 41.0 54-55 34.201708635464726 39.0 35.0 41.0 12.0 41.0 56-57 33.86436123928807 38.5 35.0 41.0 4.0 41.0 58-59 33.4370942649967 38.0 34.0 40.0 2.0 41.0 60-61 32.901326301911666 38.0 33.5 40.0 2.0 41.0 62-63 32.43185234014502 38.0 33.0 40.0 2.0 41.0 64-65 31.915042847725772 37.0 33.0 40.0 2.0 41.0 66-67 31.289429136453528 37.0 32.0 39.5 2.0 41.0 68-69 30.649518787079764 36.0 31.0 39.0 2.0 41.0 70-71 30.04809756097561 35.5 30.5 39.0 2.0 40.0 72-73 29.607198417930125 35.0 29.5 38.0 2.0 40.0 74-75 29.173473961766646 35.0 29.0 37.0 2.0 39.5 76-77 27.890515491100857 33.5 27.0 36.0 2.0 38.5 78-79 28.476403427818063 35.0 29.0 36.0 2.0 39.0 80-81 28.25029400131839 35.0 29.0 36.0 2.0 38.5 82-83 27.901737640079105 34.5 28.5 35.5 2.0 37.0 84-85 27.538652603823337 34.0 27.0 35.0 2.0 37.0 86-87 27.20520237310481 34.0 26.0 35.0 2.0 36.0 88-89 26.794249176005273 34.0 25.5 35.0 2.0 36.0 90-91 26.512899143045484 34.0 25.0 35.0 2.0 36.0 92-93 26.21295978905735 34.0 24.0 35.0 2.0 35.0 94-95 25.931870797626896 34.0 22.5 35.0 2.0 35.0 96-97 25.51697033618985 33.5 19.0 35.0 2.0 35.0 98-99 25.065065260382333 33.0 4.5 35.0 2.0 35.0 100-101 23.11963876071193 31.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29254555098038537 1101 2 0.20548473066207862 1101 3 0.41925686118120353 1101 4 0.31325102845611497 1101 5 0.2593352945756209 1101 6 0.10034952988839052 1101 7 0.2188860337723284 1101 8 0.08224888180203749 1101 9 0.09234456327704521 1101 10-11 0.11072200393919474 1101 12-13 0.319137313637917 1101 14-15 0.28878594887593323 1101 16-17 0.38618799117172387 1101 18-19 0.2572207905945021 1101 20-21 0.13487940936251164 1101 22-23 0.235893044278896 1101 24-25 0.33641836910160805 1101 26-27 0.514364030170789 1101 28-29 0.3455373182671835 1101 30-31 0.18967281336585984 1101 32-33 0.3146472954736339 1101 34-35 0.018319678143157603 1101 36-37 0.30553277354789543 1101 38-39 0.024737607747418622 1101 40-41 -0.06783186169197819 1101 42-43 -0.30946571919164967 1101 44-45 -0.1170673657344139 1101 46-47 0.07167803547870477 1101 48-49 0.27600943009471024 1101 50-51 0.04264435809194822 1101 52-53 0.12851137512544852 1101 54-55 0.29681054880957447 1101 56-57 0.7895163803007179 1101 58-59 0.39628801369297406 1101 60-61 -0.1750032707133542 1101 62-63 -8.871196686612848E-4 1101 64-65 -0.2327986946637317 1101 66-67 -0.23757280781448387 1101 68-69 0.1926001623682687 1101 70-71 0.1265351999932669 1101 72-73 0.4135309059499619 1101 74-75 0.3661774528223951 1101 76-77 0.45372690953363204 1101 78-79 0.6298193398669873 1101 80-81 0.5901129297509122 1101 82-83 0.3769711458905043 1101 84-85 0.1583042620266788 1101 86-87 0.49779534409681503 1101 88-89 0.7597908891071121 1101 90-91 0.965886535976658 1101 92-93 0.6214793190120496 1101 94-95 0.28482152939134053 1101 96-97 0.1372766584015963 1101 98-99 0.20089742262667443 1101 100-101 0.266645054542213 1102 1 0.33703658289280014 1102 2 0.21480222425630302 1102 3 0.43917300373885126 1102 4 0.4033977789802279 1102 5 0.20319739567042916 1102 6 0.23126031488722276 1102 7 0.16880450570337757 1102 8 0.19394233626278634 1102 9 0.17235853951743962 1102 10-11 0.32974133773840464 1102 12-13 0.4063141130788708 1102 14-15 0.5329625153795448 1102 16-17 0.4489063699278404 1102 18-19 0.8816675644123464 1102 20-21 0.45470237698422267 1102 22-23 0.2874304307219404 1102 24-25 0.5234670530056391 1102 26-27 0.4349323972800363 1102 28-29 0.7439766380901531 1102 30-31 0.6081447444164141 1102 32-33 0.5841557976865488 1102 34-35 0.23169028145087367 1102 36-37 0.5169816438017989 1102 38-39 0.43499849756805276 1102 40-41 0.7886787369569888 1102 42-43 1.3047900460075113 1102 44-45 1.5059235497093297 1102 46-47 1.707305801609614 1102 48-49 1.746368153598084 1102 50-51 1.8968838176773204 1102 52-53 2.3047284727261967 1102 54-55 2.153146444453647 1102 56-57 2.3259710739881214 1102 58-59 2.483348344463998 1102 60-61 2.828840195394271 1102 62-63 3.2651273224464106 1102 64-65 3.289563289962409 1102 66-67 3.112415545318516 1102 68-69 3.8120970177141835 1102 70-71 3.959402658646887 1102 72-73 3.7751661260757423 1102 74-75 4.25710454343502 1102 76-77 3.75211964188863 1102 78-79 3.606350902876539 1102 80-81 3.6210353616167374 1102 82-83 3.9257132842552878 1102 84-85 3.8879804791242805 1102 86-87 3.6170592621028703 1102 88-89 3.677564008138095 1102 90-91 3.265851595375679 1102 92-93 3.387493761127118 1102 94-95 2.910432989908461 1102 96-97 2.8122708349681034 1102 98-99 3.0854769707282372 1102 100-101 3.4570713298741467 1103 1 -0.18093537873890853 1103 2 -0.14122883579077694 1103 3 -0.15026517482127844 1103 4 -0.2205788908486639 1103 5 -0.26628716214553094 1103 6 0.04103509441007702 1103 7 -0.06534046460686938 1103 8 -0.4054744094594298 1103 9 -0.2172974810018644 1103 10-11 -0.3585689816460089 1103 12-13 -0.3626855706989289 1103 14-15 -0.22892216892529405 1103 16-17 -0.3764335689358731 1103 18-19 -0.20903550294298867 1103 20-21 -0.40419225075198284 1103 22-23 -0.07193853031245823 1103 24-25 -0.16840955006522762 1103 26-27 -1.5421246663294141E-4 1103 28-29 -0.26243564538548014 1103 30-31 -0.3459503330580276 1103 32-33 0.3007153001636169 1103 34-35 0.151949583653888 1103 36-37 0.014012668713078824 1103 38-39 0.3972977843113128 1103 40-41 0.03431311382357194 1103 42-43 0.013935894712759023 1103 44-45 0.147468119471462 1103 46-47 0.07986825549388499 1103 48-49 0.19988349037472375 1103 50-51 0.3694427579816022 1103 52-53 0.32997354493026165 1103 54-55 0.49294476496241657 1103 56-57 0.6106764603062373 1103 58-59 0.610768322679796 1103 60-61 0.5579783142235399 1103 62-63 0.7578893476739168 1103 64-65 0.9069695652162579 1103 66-67 0.9905118876817518 1103 68-69 0.9132705534297223 1103 70-71 0.9407982526175864 1103 72-73 1.0485402169369245 1103 74-75 0.8844718179510238 1103 76-77 0.7264353721862307 1103 78-79 0.8513001316457327 1103 80-81 0.768728696355506 1103 82-83 1.0580698423523316 1103 84-85 0.7601388713100796 1103 86-87 0.9868635556976173 1103 88-89 0.8803854128673727 1103 90-91 0.6329152965808333 1103 92-93 1.4770900507866536 1103 94-95 1.2875630977753652 1103 96-97 1.3514121160193646 1103 98-99 1.226002602294237 1103 100-101 1.6796390541283905 1104 1 0.11804145887329298 1104 2 -0.05987801035609408 1104 3 0.1333642269739599 1104 4 0.04296388738245582 1104 5 0.18782745715017768 1104 6 0.08717155790891695 1104 7 0.07545421243789008 1104 8 0.18309504631861273 1104 9 -0.270119023905643 1104 10-11 0.24239768711300513 1104 12-13 0.05269952753721441 1104 14-15 0.20510582680630307 1104 16-17 0.19413638900775965 1104 18-19 0.3853245497844 1104 20-21 0.18894377335197277 1104 22-23 0.3199546339584671 1104 24-25 0.1719017926000177 1104 26-27 0.011693756901266283 1104 28-29 0.47731703806188364 1104 30-31 0.30466795832355587 1104 32-33 0.45859929991253523 1104 34-35 0.49806721594313785 1104 36-37 0.2260834740611415 1104 38-39 0.441959136392164 1104 40-41 0.12699405036239853 1104 42-43 0.22580269529025543 1104 44-45 0.14437666256355186 1104 46-47 0.7729195391546639 1104 48-49 0.7449371593220562 1104 50-51 0.7304998165466614 1104 52-53 0.9528628801885688 1104 54-55 0.9353526489621657 1104 56-57 1.0184663040071982 1104 58-59 1.2717908573606067 1104 60-61 0.5866488308263911 1104 62-63 0.18686430216515504 1104 64-65 0.3816415883158797 1104 66-67 0.8019957869975549 1104 68-69 0.7066451061884926 1104 70-71 0.6493538988419303 1104 72-73 0.6766395201821709 1104 74-75 0.4616483919262926 1104 76-77 0.5340431808611399 1104 78-79 1.0421858262035144 1104 80-81 0.92407048280959 1104 82-83 0.8001921196732802 1104 84-85 0.8468879768276203 1104 86-87 0.7386054425848165 1104 88-89 0.4520852433864846 1104 90-91 0.6847795863303787 1104 92-93 0.6753356097597241 1104 94-95 0.5309721744183875 1104 96-97 0.6125853003769066 1104 98-99 0.9164959799855339 1104 100-101 0.8847266192529908 1105 1 -0.11076899545010832 1105 2 -0.09993663273029796 1105 3 -0.049342172635221004 1105 4 0.015545714446453474 1105 5 0.11870190756542343 1105 6 0.024396765529829167 1105 7 -0.08331471770378585 1105 8 0.10090159248372998 1105 9 -0.19006284413035957 1105 10-11 0.019907457508715254 1105 12-13 -0.007754795862886965 1105 14-15 0.13094241693819697 1105 16-17 -0.12810347420669643 1105 18-19 -0.2551248884178463 1105 20-21 -0.275882802124336 1105 22-23 -0.11968264010113927 1105 24-25 -0.18676332708733412 1105 26-27 -0.1906144990831038 1105 28-29 -0.24004373379937505 1105 30-31 -0.14241924245808235 1105 32-33 -0.047201872534948563 1105 34-35 -0.033075920353880406 1105 36-37 -0.1666559006335362 1105 38-39 0.14866407044198837 1105 40-41 -0.28626315530443236 1105 42-43 -0.4972676515588006 1105 44-45 -0.04953515961525312 1105 46-47 -0.08217327960449694 1105 48-49 0.04717824383597957 1105 50-51 0.2588950290527663 1105 52-53 0.4339327385178322 1105 54-55 0.6546674999636295 1105 56-57 0.6735408032744132 1105 58-59 0.5927788886258796 1105 60-61 0.48180027195976294 1105 62-63 0.009043100406486815 1105 64-65 -0.16888967695036783 1105 66-67 -0.018839581834882324 1105 68-69 0.431730597150878 1105 70-71 0.8369149638150617 1105 72-73 0.20882076102242664 1105 74-75 0.5003025199184776 1105 76-77 0.13257151500867437 1105 78-79 0.16984215253473067 1105 80-81 0.5771298379634722 1105 82-83 0.8923696966151446 1105 84-85 0.8133633065784771 1105 86-87 0.9947424723845231 1105 88-89 0.9520547108999189 1105 90-91 0.8440065037705367 1105 92-93 0.84698924923212 1105 94-95 1.126508324882483 1105 96-97 1.0337809525508206 1105 98-99 1.296442242809178 1105 100-101 0.9050307228191805 1106 1 -0.19354931581624157 1106 2 -0.1479915526387643 1106 3 -0.06787764174277555 1106 4 -0.1259302580935895 1106 5 -0.2776367652034537 1106 6 -0.18704488206742553 1106 7 -0.02679297857334717 1106 8 -0.4024164853194705 1106 9 -0.5519049493935171 1106 10-11 -0.2858991786468934 1106 12-13 -0.4749172672587676 1106 14-15 -0.7727475144119182 1106 16-17 -0.785254503954981 1106 18-19 -0.7919669936810081 1106 20-21 -0.8245098044126635 1106 22-23 -0.6026059810622328 1106 24-25 -0.4148811760575839 1106 26-27 0.01722302952101984 1106 28-29 -0.2805185621746631 1106 30-31 -0.22179567266402245 1106 32-33 -0.4447419183015384 1106 34-35 -0.07804159541109357 1106 36-37 -0.3451169990317027 1106 38-39 -0.6155807808165932 1106 40-41 -0.6207883269291443 1106 42-43 -0.6878282922910586 1106 44-45 -0.21804202460381106 1106 46-47 -0.49115497296833865 1106 48-49 -0.430195898040445 1106 50-51 -0.3539768244941399 1106 52-53 -0.5048544468368661 1106 54-55 -0.5714480606770991 1106 56-57 -0.33381045530681774 1106 58-59 0.11191504423228338 1106 60-61 0.046731622074169366 1106 62-63 0.19771586928520435 1106 64-65 0.007454716642314452 1106 66-67 0.3779567568378859 1106 68-69 0.5820738465787976 1106 70-71 0.5257879615267314 1106 72-73 0.462653712967505 1106 74-75 0.836515334563785 1106 76-77 0.6222454280521497 1106 78-79 0.6289096582555551 1106 80-81 0.1705222864760607 1106 82-83 0.37346237396068105 1106 84-85 0.5977454576082231 1106 86-87 0.20049190030214348 1106 88-89 0.4309734752019807 1106 90-91 -0.009454594627634094 1106 92-93 0.8499068693694234 1106 94-95 0.12127377110673976 1106 96-97 0.4336379319558539 1106 98-99 0.7681813732439586 1106 100-101 0.24247351458119581 1107 1 0.13149055659205544 1107 2 0.17630403481472 1107 3 0.12632756825190938 1107 4 0.23749345269854416 1107 5 -0.01788131597432141 1107 6 0.11157287444506636 1107 7 0.1358332840529144 1107 8 -0.11023147734498906 1107 9 -0.274658803726318 1107 10-11 0.020374080257944627 1107 12-13 0.06492855942916975 1107 14-15 -0.02883470207805061 1107 16-17 0.15554826053199378 1107 18-19 0.2423498590569011 1107 20-21 0.49234293349270075 1107 22-23 0.5549166133024599 1107 24-25 0.48344418279408785 1107 26-27 0.47424057338066206 1107 28-29 0.2610816504781823 1107 30-31 0.2833877404141205 1107 32-33 0.5761512236442314 1107 34-35 0.5758393189961382 1107 36-37 0.5078335591820107 1107 38-39 0.20767812402126395 1107 40-41 0.09266196546851546 1107 42-43 0.07184741212148538 1107 44-45 0.17754542887838198 1107 46-47 0.6637094608434637 1107 48-49 0.48841122582983587 1107 50-51 0.596235828350089 1107 52-53 0.4228435861243298 1107 54-55 0.264543433209127 1107 56-57 0.17812468557568906 1107 58-59 0.15330260404089557 1107 60-61 -0.2069898026998871 1107 62-63 -0.011268713383593365 1107 64-65 0.0769656151005762 1107 66-67 -0.12028885719719895 1107 68-69 6.809704490784441E-4 1107 70-71 0.33304473983614 1107 72-73 0.4690864615055155 1107 74-75 0.43492043823204796 1107 76-77 0.2879019937087186 1107 78-79 0.5024343791486956 1107 80-81 0.39566848530646936 1107 82-83 0.3163650852844455 1107 84-85 0.015767786156867913 1107 86-87 0.16418142714956474 1107 88-89 0.5294317420812966 1107 90-91 0.46925353934366143 1107 92-93 0.5984714626708545 1107 94-95 0.2461009906708007 1107 96-97 0.5671307549223599 1107 98-99 0.9217167267793158 1107 100-101 0.9568807447779015 1108 1 -0.38886222495475664 1108 2 2.1478409860264946E-4 1108 3 -0.22336337099127235 1108 4 -0.20084515728186147 1108 5 0.12021607585440819 1108 6 0.13720230305956704 1108 7 -0.1218178541952426 1108 8 -0.04148430412020332 1108 9 -0.4071026451253843 1108 10-11 -0.09183005060290128 1108 12-13 0.1316027641630697 1108 14-15 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-1.5649519635998956 2316 72-73 -1.2468596100890927 2316 74-75 -1.5768077220316776 2316 76-77 -1.4001395091863422 2316 78-79 -1.9317928301831166 2316 80-81 -1.5975904640194258 2316 82-83 -1.7426243910690467 2316 84-85 -1.5284822455408609 2316 86-87 -1.6411839420233356 2316 88-89 -1.7503852563549458 2316 90-91 -1.320685601013892 2316 92-93 -1.3252637978844852 2316 94-95 -1.424189783176459 2316 96-97 -1.45331428121305 2316 98-99 -1.386839547258134 2316 100-101 -1.4389182493474522 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 1.0 5 12.0 6 27.0 7 49.0 8 130.0 9 168.0 10 321.0 11 505.0 12 865.0 13 1271.0 14 1499.0 15 2087.0 16 2681.0 17 3605.0 18 3540.0 19 2494.0 20 2126.0 21 2211.0 22 2486.0 23 2457.0 24 2630.0 25 2884.0 26 2463.0 27 1945.0 28 2180.0 29 2620.0 30 2993.0 31 3657.0 32 4522.0 33 5798.0 34 8254.0 35 15276.0 36 18372.0 37 35705.0 38 45359.0 39 6430.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.688859591298616 24.669742913645354 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31.963612392880687 33.93144363876071 18.112854317732367 20-21 17.381938035596573 30.546077785102177 33.66170072511536 18.410283454185894 22-23 17.29439683586025 30.892023731048123 34.32458800263679 17.488991430454845 24-25 17.987933507720868 32.08168087081259 33.722628892076614 16.207756729389924 26-27 16.733300983163847 31.48471454491751 35.33719777205789 16.444786699860753 28-29 15.512458800263678 31.417270929466053 35.6569545154911 17.41331575477917 30-31 16.76018457481872 31.10112063282795 35.29940672379696 16.83928806855636 32-33 15.667015601886888 32.59520789771364 35.24465456731593 16.49312193308354 34-35 16.134774685440462 31.91140906321783 35.553205461244104 16.400610790097605 36-37 16.325766465123763 31.29118423377219 34.95560870264555 17.4274405984585 38-39 15.627563035847558 31.107178741999377 35.72809337823618 17.537164843916887 40-41 15.258680253379536 31.360200847059815 36.02720507181021 17.35391382775044 42-43 15.995601532607463 30.798237448677956 34.88211421775578 18.3240468009588 44-45 15.56142696294421 30.76107678798858 35.10952196372209 18.567974285345127 46-47 17.4513314471495 30.5361385264035 34.44227934681437 17.570250679632643 48-49 16.647330257086352 30.199077125906392 34.569545154911005 18.584047462096244 50-51 15.109822017139091 30.343836519446278 35.5085036255768 19.037837837837838 52-53 15.671984179301251 31.29676994067238 34.95978905735003 18.071456822676335 54-55 16.12076466710613 30.191430454845086 33.846802900461434 19.841001977587343 56-57 17.311008569545155 30.695847066578775 32.211997363216874 19.781147000659196 58-59 16.586420566908373 31.066315095583384 33.66433750823995 18.682926829268293 60-61 16.761766644693473 33.02808174027686 31.931179960448254 18.27897165458141 62-63 15.456295319709954 33.57152274225445 32.16058009228741 18.81160184574819 64-65 15.805141727092947 33.30336189848385 31.624258404746207 19.267237969676994 66-67 16.090969017798287 32.809492419248514 30.40290046143705 20.69663810151615 68-69 15.837837837837837 31.82702702702703 31.735003295978903 20.60013183915623 70-71 17.140936058009228 30.75886618325643 31.44442979564931 20.655767963085037 72-73 16.275807514831904 30.11891891891892 32.29927488464074 21.305998681608436 74-75 16.028213579433093 31.342386288727752 30.98061964403428 21.648780487804878 76-77 16.13843111404087 30.14001318391562 32.10916282135795 21.61239288068556 78-79 16.220962425840472 29.744232036914966 32.250230718523405 21.78457481872116 80-81 17.064996704021095 29.97996044825313 31.0502307185234 21.904812129202373 82-83 16.818984838497034 29.397231377719184 31.783256427158868 22.000527356624918 84-85 16.246654449088297 29.317441691826968 31.69169512307672 22.744208736008016 86-87 16.192527864279143 29.895925895651672 31.43712969579353 22.47441654427566 88-89 16.529993408042188 29.29703361898484 30.803164139749505 23.36980883322347 90-91 16.684508899143047 28.156624917600524 31.303625576796307 23.855240606460118 92-93 17.26987475280158 28.871456822676333 31.146736980883322 22.71193144363876 94-95 16.408437705998683 27.91852340145023 31.90323005932762 23.76980883322347 96-97 16.14739617666447 28.205141727092947 31.782465392221486 23.864996704021095 98-99 16.552669742913643 28.56242584047462 31.02597231377719 23.858932102834544 100-101 16.03717864205669 28.08595912986157 31.45550428477258 24.421357943309165 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3268.0 1 1845.0 2 537.5 3 676.5 4 496.0 5 377.0 6 738.5 7 1289.0 8 1656.0 9 1673.0 10 1240.5 11 774.0 12 640.5 13 606.0 14 599.5 15 617.5 16 613.5 17 583.0 18 628.5 19 699.0 20 744.0 21 820.0 22 884.5 23 972.5 24 1064.0 25 1226.0 26 1507.5 27 1775.0 28 1936.5 29 2294.0 30 3011.0 31 3552.5 32 4451.0 33 5664.5 34 6168.5 35 6965.0 36 9040.0 37 10397.5 38 9992.0 39 9206.5 40 8550.5 41 7537.5 42 6294.5 43 6061.5 44 6495.5 45 6697.0 46 6092.0 47 6468.0 48 8218.0 49 7695.0 50 5916.0 51 4397.5 52 2648.0 53 1505.0 54 1056.5 55 865.5 56 701.5 57 580.0 58 503.5 59 418.0 60 337.0 61 273.5 62 212.0 63 149.5 64 88.5 65 63.5 66 51.5 67 41.5 68 27.5 69 16.0 70 13.5 71 12.0 72 10.0 73 7.5 74 6.0 75 6.0 76 4.0 77 3.0 78 2.0 79 0.5 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.017402768622280816 3 0.005273566249176006 4 0.0 5 0.0 6 0.0 7 0.0 8 5.273566249176005E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004746209624258405 26-27 0.017402768622280816 28-29 0.0 30-31 0.0 32-33 2.6367831245880025E-4 34-35 0.018193803559657218 36-37 0.0023731048121292024 38-39 0.016084377059986815 40-41 0.014765985497692815 42-43 0.007119314436387607 44-45 0.002900461437046803 46-47 2.6367831245880025E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.003955174686882004 86-87 2.6367831245880025E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 189625.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.88688200395518 #Duplication Level Percentage of deduplicated Percentage of total 1 77.90158423853839 26.398417930125245 2 9.296896884434624 6.300856954515491 3 3.513959351364811 3.572313777191826 4 1.8985962837312087 2.573500329597891 5 1.2231939991907623 2.07251153592617 6 0.8714868187618663 1.771918259723138 7 0.6925207756232686 1.6427158866183258 8 0.49488001493977407 1.3415952537903757 9 0.40306265367736316 1.2292682926829268 >10 3.1280151887702696 21.639024390243904 >50 0.3921690684428398 9.018325642715887 >100 0.16029132559370038 10.050889914304548 >500 0.009337358772448567 2.291891891891892 >1k 0.014006038158672851 10.096769940672381 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3531 1.8620962425840475 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3217 1.696506262359921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3010 1.5873434410019778 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2227 1.1744232036914966 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1915 1.009887936717205 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1591 0.8390243902439025 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1372 0.7235332893869479 TruSeq Adapter, Index 11 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1219 0.6428477257745551 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1064 0.5611074489123269 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 876 0.4619644034278181 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 791 0.417139090309822 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 748 0.3944627554383652 RNA PCR Primer, Index 11 (95% over 23bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 696 0.36704021094265 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 667 0.3517468688200396 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 568 0.2995385629531971 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 483 0.25471324983520105 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 479 0.25260382333553066 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 473 0.24943968358602506 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 414 0.21832564271588661 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 411 0.21674357284113382 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 411 0.21674357284113382 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 410 0.21621621621621623 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 403 0.21252471984179302 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 381 0.20092287409360582 TruSeq Adapter, Index 11 (96% over 28bp) CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 356 0.18773895847066577 TruSeq Adapter, Index 11 (96% over 26bp) GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 339 0.17877389584706657 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 319 0.16822676334871456 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 315 0.16611733684904417 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 303 0.15978905735003296 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 283 0.14924192485168097 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 282 0.14871456822676335 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 268 0.14133157547791694 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 264 0.13922214897824653 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 254 0.13394858272907054 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 251 0.13236651285431772 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 245 0.12920237310481214 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 230 0.12129202373104814 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 217 0.1144363876071193 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 215 0.1133816743572841 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 214 0.11285431773236651 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 209 0.11021753460777851 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 207 0.10916282135794331 TruSeq Adapter, Index 11 (95% over 24bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 205 0.10810810810810811 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 204 0.1075807514831905 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 201 0.10599868160843771 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 192 0.1012524719841793 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 190 0.10019775873434411 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.6367831245880025E-4 0.0 18-19 0.0 0.0 0.0 5.273566249176005E-4 0.0 20-21 0.0 0.0 0.0 0.0023731048121292024 0.0 22-23 0.0 0.0 0.0 0.007646671061305208 0.0 24-25 0.0 0.0 0.0 0.02109426499670402 0.0 26-27 0.0 0.0 0.0 0.049571522742254445 0.0 28-29 0.0 0.0 0.0 0.12577455504284774 0.0 30-31 0.0 0.0 0.0 0.2913645352669743 0.0 32-33 0.0 0.0 0.0 0.5286750164798946 0.0 34-35 0.0 0.0 0.0 0.9075807514831905 0.0 36-37 0.0 0.0 0.0 1.4183256427158866 0.0 38-39 0.0 0.0 0.0 1.97363216875412 0.0 40-41 0.0 0.0 0.0 2.456954515491101 0.0 42-43 0.0 0.0 0.0 2.950560316413975 0.0 44-45 0.0 0.0 0.0 3.4918918918918918 0.0 46-47 0.0 0.0 0.0 4.108371786420567 0.0 48-49 0.0 0.0 0.0 4.61753460777851 0.0 50-51 0.0 0.0 0.0 5.117732366512854 0.0 52-53 0.0 0.0 0.0 5.580487804878048 0.0 54-55 0.0 0.0 0.0 6.081740276862227 0.0 56-57 0.0 0.0 0.0 6.6705339485827295 0.0 58-59 0.0 0.0 0.0 7.224785761371127 0.0 60-61 0.0 0.0 0.0 7.744495715227423 0.0 62-63 0.0 0.0 0.0 8.231245880026368 0.0 64-65 0.0 0.0 0.0 8.723533289386948 0.0 66-67 0.0 0.0 0.0 9.299670402109427 0.0 68-69 0.0 0.0 0.0 9.89848384970336 0.0 70-71 0.0 0.0 0.0 10.520500988793671 0.0 72-73 0.0 0.0 0.0 11.076334871456822 0.0 74-75 0.0 0.0 0.0 11.685168094924192 0.0 76-77 0.0 0.0 0.0 12.308239947264337 0.0 78-79 0.0 0.0 0.0 13.044957152274225 0.0 80-81 0.0 0.0 0.0 13.78668424522083 0.0 82-83 0.0 0.0 0.0 14.458536585365854 0.0 84-85 0.0 0.0 0.0 15.161502966381015 0.0 86-87 0.0 0.0 0.0 15.809360580092289 0.0 88-89 0.0 0.0 0.0 16.499406723796966 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTAGC 35 3.4072564E-6 67.853004 1 TGGACAG 25 0.0050160405 56.996517 5 TGTATCC 25 0.0050160405 56.996517 8 GGATGAC 65 2.578976E-6 43.843475 8 GATGACC 80 1.073322E-5 35.622826 9 GGGATGA 95 8.6532054E-7 34.997864 7 ATGGGAT 150 9.440555E-10 31.664732 5 CGCAGAA 120 1.4953912E-7 31.664732 9 TTGAGAC 40 4.4826992E-4 29.693518 38-39 TATCACC 65 0.005833672 29.228985 3 GTAGCCC 65 0.005833672 29.228985 3 CTGGCTG 65 0.005833672 29.228985 9 ACCAAGC 85 6.389593E-4 27.93947 7 TCTGTAG 35 0.008376317 27.14836 28-29 TAGCCCT 135 1.3356828E-5 24.628126 4 CCTATTC 160 1.8469273E-6 23.74855 3 CATGGGG 265 3.092282E-11 23.300465 6 TATTCCC 125 2.2471987E-4 22.798607 5 TCCCATC 125 2.2471987E-4 22.798607 8 AGCAGAT 105 0.0021902898 22.617666 7 >>END_MODULE