##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938056_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 184715 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.343902769130825 33.0 31.0 34.0 30.0 34.0 2 31.43845383428525 33.0 31.0 34.0 30.0 34.0 3 31.384857753836993 34.0 31.0 34.0 28.0 34.0 4 34.85380721652275 37.0 35.0 37.0 33.0 37.0 5 34.86931759737975 37.0 35.0 37.0 33.0 37.0 6 34.95528787591695 37.0 35.0 37.0 33.0 37.0 7 34.906618303873536 37.0 35.0 37.0 33.0 37.0 8 34.912183634247356 37.0 35.0 37.0 33.0 37.0 9 36.47334542403161 39.0 37.0 39.0 33.0 39.0 10-11 36.41948136318112 39.0 37.0 39.0 33.0 39.0 12-13 36.32495195300869 39.0 37.0 39.0 32.0 39.0 14-15 37.38829818910213 40.0 38.0 41.0 32.0 41.0 16-17 37.25015293831037 40.0 38.0 41.0 32.0 41.0 18-19 37.40140216008445 40.0 38.0 41.0 33.0 41.0 20-21 37.41767046531143 40.0 38.0 41.0 33.0 41.0 22-23 37.27576807514279 40.0 38.0 41.0 32.5 41.0 24-25 37.142351731045125 40.0 37.0 41.0 32.0 41.0 26-27 36.9247191619522 40.0 37.0 41.0 32.0 41.0 28-29 36.712600492650836 40.0 36.0 41.0 31.5 41.0 30-31 36.43163522182822 39.5 35.5 41.0 31.0 41.0 32-33 35.99136236905503 39.0 35.0 41.0 30.0 41.0 34-35 35.708737785236714 39.0 35.0 41.0 30.0 41.0 36-37 35.42025552878759 38.5 35.0 41.0 29.0 41.0 38-39 35.101191024009964 38.0 35.0 40.5 26.5 41.0 40-41 34.87146414747043 38.5 35.0 41.0 24.5 41.0 42-43 34.54224886988062 38.5 35.0 41.0 22.5 41.0 44-45 34.08725062934792 38.0 35.0 40.5 13.5 41.0 46-47 33.54727011883172 38.0 34.5 40.0 2.0 41.0 48-49 33.02431042416696 38.0 34.0 40.0 2.0 41.0 50-51 32.2584738651436 37.5 33.0 39.5 2.0 40.5 52-53 32.28094902958612 38.0 33.0 40.0 2.0 40.5 54-55 32.3446336247733 38.0 33.0 40.0 2.0 41.0 56-57 32.04229759359012 38.0 33.0 40.0 2.0 41.0 58-59 31.670846980483446 38.0 32.5 40.0 2.0 41.0 60-61 31.207592778063503 37.5 31.5 40.0 2.0 41.0 62-63 30.74706981024822 37.0 31.0 40.0 2.0 41.0 64-65 30.283618006117532 36.0 29.5 39.5 2.0 41.0 66-67 29.793936605040198 36.0 28.5 39.0 2.0 41.0 68-69 29.205963240668055 35.0 28.0 39.0 2.0 40.0 70-71 28.6968816825921 35.0 26.5 38.0 2.0 40.0 72-73 28.066526811574587 35.0 26.0 37.5 2.0 39.5 74-75 27.50521073004358 34.0 24.5 37.0 2.0 39.0 76-77 26.91024280648567 34.0 22.0 36.0 2.0 39.0 78-79 26.243169206615597 34.0 19.0 36.0 2.0 38.0 80-81 25.610010015429175 33.0 12.0 35.0 2.0 37.0 82-83 24.995287334542404 32.5 6.5 35.0 2.0 37.0 84-85 24.359072084021328 32.0 2.0 35.0 2.0 36.0 86-87 23.75850364074385 31.5 2.0 35.0 2.0 36.0 88-89 23.015523915220747 31.0 2.0 34.5 2.0 35.0 90-91 22.256067455268926 30.5 2.0 34.0 2.0 35.0 92-93 21.458804103619087 29.5 2.0 34.0 2.0 35.0 94-95 20.576661343150256 29.0 2.0 34.0 2.0 35.0 96-97 19.518542078336896 28.0 2.0 34.0 2.0 35.0 98-99 18.247437944942206 25.5 2.0 34.0 2.0 35.0 100-101 14.526248545055898 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.35516488405533764 1101 2 0.5538677655633037 1101 3 0.1180840663848457 1101 4 -0.25034295491708036 1101 5 0.1050814581395727 1101 6 0.23386499962281704 1101 7 0.317468793779895 1101 8 0.2502227980769902 1101 9 0.1653392072233686 1101 10-11 0.4343646044842231 1101 12-13 0.3347830965928509 1101 14-15 0.5431941627517247 1101 16-17 0.6822312957213583 1101 18-19 0.5338902599261104 1101 20-21 0.37308023100374044 1101 22-23 0.2624328753914611 1101 24-25 0.4558194018046038 1101 26-27 0.3699203413303138 1101 28-29 0.667275202730572 1101 30-31 0.5756403806244705 1101 32-33 0.5572888536806673 1101 34-35 0.8613260015722517 1101 36-37 0.6830549606205949 1101 38-39 0.5267531528777525 1101 40-41 0.3504647413797102 1101 42-43 -0.16882858759311148 1101 44-45 -0.3444926358967919 1101 46-47 -0.0988320315695077 1101 48-49 0.4429030836318546 1101 50-51 0.11978323529265111 1101 52-53 0.15481939307085213 1101 54-55 0.5523371392278946 1101 56-57 0.40055550037077126 1101 58-59 0.7015616061122891 1101 60-61 0.3394875438675342 1101 62-63 0.5245049959258061 1101 64-65 0.4360202578896697 1101 66-67 0.49882562732435076 1101 68-69 0.6388479454618157 1101 70-71 0.5818160738767801 1101 72-73 0.7805338660878327 1101 74-75 -0.06301277969052776 1101 76-77 -0.08843176185893498 1101 78-79 -0.18926982916313762 1101 80-81 -0.3031921515282754 1101 82-83 -0.6599953165768042 1101 84-85 -0.8378922713088066 1101 86-87 -0.69456101394006 1101 88-89 -0.7008476929627783 1101 90-91 -0.27716897011545427 1101 92-93 -0.042273655218728834 1101 94-95 -0.45619808852140054 1101 96-97 -0.7072537828202883 1101 98-99 -1.0618050186526595 1101 100-101 -2.6632311554797976 1102 1 0.3509434956876092 1102 2 0.34623799695792457 1102 3 0.7586406642585146 1102 4 0.4962173953018123 1102 5 0.26424343437471975 1102 6 0.3114134674151856 1102 7 0.6608083810219227 1102 8 0.4003166067073849 1102 9 0.8073029345529505 1102 10-11 0.792322703295973 1102 12-13 0.7817030465615815 1102 14-15 0.9577501352345195 1102 16-17 0.802462690342999 1102 18-19 0.3927082711831389 1102 20-21 0.6264417069261938 1102 22-23 1.015325308161941 1102 24-25 0.9650439171266783 1102 26-27 1.0432317859832239 1102 28-29 0.9992014066080017 1102 30-31 0.8124289985106543 1102 32-33 1.037198172004615 1102 34-35 1.2748657138924528 1102 36-37 1.3376828530533658 1102 38-39 1.1745017457483016 1102 40-41 1.455374059703658 1102 42-43 1.8328912372974457 1102 44-45 1.906758145841792 1102 46-47 1.4079690941340601 1102 48-49 1.6204359166524966 1102 50-51 1.319986487325167 1102 52-53 1.126442282376665 1102 54-55 1.0037129991403404 1102 56-57 0.8912528111900428 1102 58-59 0.8873026942923978 1102 60-61 1.5858133725104366 1102 62-63 1.5848552148126167 1102 64-65 1.327827637501926 1102 66-67 1.8161333196320442 1102 68-69 1.6529192400209176 1102 70-71 1.2593489068974186 1102 72-73 0.7830354295650075 1102 74-75 1.050964706238176 1102 76-77 1.0560335289478182 1102 78-79 0.5114806398800162 1102 80-81 0.6352850217049948 1102 82-83 0.5444293238234508 1102 84-85 0.6054316811614875 1102 86-87 0.6142257277735084 1102 88-89 0.857782701033468 1102 90-91 0.8737847509602155 1102 92-93 0.9672635555379934 1102 94-95 1.47555613286697 1102 96-97 0.15484753050053257 1102 98-99 -0.6839907597408406 1102 100-101 -0.5114175219588475 1103 1 0.10791213680259304 1103 2 0.26174322343876355 1103 3 0.4042562275570063 1103 4 0.29819184361772244 1103 5 0.2667114947696092 1103 6 0.07956097031878784 1103 7 0.3083112846223841 1103 8 0.3450030178572163 1103 9 0.18273847462263149 1103 10-11 0.0701246777442961 1103 12-13 0.3746182614280187 1103 14-15 0.4374249319824912 1103 16-17 0.5219748854649495 1103 18-19 0.4104928240286725 1103 20-21 0.5640142969378061 1103 22-23 0.5598229852815706 1103 24-25 0.2877791087643047 1103 26-27 0.11625733766730662 1103 28-29 0.15033381078918495 1103 30-31 -0.21602628604219376 1103 32-33 0.39840606979787907 1103 34-35 0.5144853422315947 1103 36-37 0.6416172316828721 1103 38-39 -0.08428164565704321 1103 40-41 0.16182005273502398 1103 42-43 0.4259516321871075 1103 44-45 0.7704341040299454 1103 46-47 0.751900569163098 1103 48-49 0.6091118748406501 1103 50-51 0.1554975210069358 1103 52-53 0.15046957622102752 1103 54-55 0.08530417219492392 1103 56-57 -0.03717343735816314 1103 58-59 0.3455634376141212 1103 60-61 0.49630988818988087 1103 62-63 -0.08355361213279977 1103 64-65 0.04545249232190329 1103 66-67 -0.07374763274890839 1103 68-69 0.23088091249478637 1103 70-71 -0.3234037063429973 1103 72-73 0.028244488798453204 1103 74-75 -0.16695050862825767 1103 76-77 -0.5405837765109496 1103 78-79 -0.22567092806423616 1103 80-81 -0.010838671674793687 1103 82-83 0.04856695448546944 1103 84-85 0.15798684957031384 1103 86-87 -0.3882423326213811 1103 88-89 -0.4691627112777965 1103 90-91 -0.6290462594927462 1103 92-93 -0.07295131089638218 1103 94-95 -1.3641651214884298 1103 96-97 -1.4091768597433685 1103 98-99 -0.3850650919127325 1103 100-101 -1.4111707159193578 1104 1 0.09817187706233099 1104 2 0.2690484182439583 1104 3 0.039267050067827824 1104 4 0.2530078609337352 1104 5 0.23722015277826358 1104 6 0.2781540439118615 1104 7 0.1968394231505286 1104 8 0.2589640568182503 1104 9 0.24605016293432413 1104 10-11 0.1531874483070581 1104 12-13 -0.009039747229991235 1104 14-15 0.08136432592188214 1104 16-17 0.5702703400104028 1104 18-19 0.494773127058977 1104 20-21 0.41074048741399594 1104 22-23 0.33877320173178305 1104 24-25 0.18469469317989962 1104 26-27 0.3887140476240205 1104 28-29 0.020057836763207604 1104 30-31 0.2565115927456887 1104 32-33 -0.02218375704195097 1104 34-35 -0.3145189867727396 1104 36-37 -0.3715050626894296 1104 38-39 -0.3762188750942741 1104 40-41 0.20281031247527892 1104 42-43 0.37210964084511033 1104 44-45 0.26921657156240997 1104 46-47 0.293431954444479 1104 48-49 0.43459888782766853 1104 50-51 0.6841771746865888 1104 52-53 0.9338840783855318 1104 54-55 0.5612240856148318 1104 56-57 0.5479185539838269 1104 58-59 0.33311755016823597 1104 60-61 -0.04549205986207028 1104 62-63 -0.43717915325834156 1104 64-65 -0.5631784600590493 1104 66-67 -0.7406848621861357 1104 68-69 -0.052804152440280916 1104 70-71 -0.07989721283650653 1104 72-73 0.1556795537335205 1104 74-75 -0.24636068178843118 1104 76-77 -0.6382569366841118 1104 78-79 -0.3409306683239741 1104 80-81 -0.953614645700771 1104 82-83 -1.6226992792807664 1104 84-85 -1.6332739729405077 1104 86-87 -0.9951146269936757 1104 88-89 -0.9023337069487916 1104 90-91 -0.54057223351872 1104 92-93 -0.43198810743318106 1104 94-95 -1.1222820046053137 1104 96-97 -1.355199587016095 1104 98-99 -0.9757036200512594 1104 100-101 -1.0055971228457636 1105 1 0.5458003355603545 1105 2 0.45506620480522386 1105 3 0.8364508445342338 1105 4 0.6954485723961881 1105 5 0.8189889274818256 1105 6 0.4431738067577129 1105 7 0.5135390278936072 1105 8 0.8434502228261564 1105 9 0.4505956174797774 1105 10-11 0.40565780403829166 1105 12-13 0.3499029405170475 1105 14-15 0.5252872508230695 1105 16-17 0.9268661898127775 1105 18-19 1.003789925477946 1105 20-21 0.8360122265444332 1105 22-23 0.8842277471863298 1105 24-25 1.0253567485158612 1105 26-27 0.9662832175844898 1105 28-29 1.186436295261231 1105 30-31 1.057524438595486 1105 32-33 0.4633646619303846 1105 34-35 0.6568248867450492 1105 36-37 0.33662240766630447 1105 38-39 0.36266452411521044 1105 40-41 1.0092579409337787 1105 42-43 0.9343626052719927 1105 44-45 1.3768005636572767 1105 46-47 1.2145040888318377 1105 48-49 1.0035218127288488 1105 50-51 0.3462325106549713 1105 52-53 0.2959147107966018 1105 54-55 0.4006509630851909 1105 56-57 0.7098503721656471 1105 58-59 0.7972973920654702 1105 60-61 0.7152539875687651 1105 62-63 0.8187497004965998 1105 64-65 0.6338324095061694 1105 66-67 0.6693941891972628 1105 68-69 0.8974181795755278 1105 70-71 0.3978695855824661 1105 72-73 0.5709463521524931 1105 74-75 0.6648300296740182 1105 76-77 0.8866936562012633 1105 78-79 0.8799191340475652 1105 80-81 0.979068160623342 1105 82-83 0.8649489420631085 1105 84-85 0.5627487543322189 1105 86-87 -0.043780634898812565 1105 88-89 0.4195536448298647 1105 90-91 -0.23968290545547788 1105 92-93 0.10976090442452957 1105 94-95 0.21541562385318613 1105 96-97 0.10984487938706877 1105 98-99 0.012191636865733102 1105 100-101 -0.48039949438726737 1106 1 -0.027181030899271263 1106 2 0.04435191909093561 1106 3 0.21455643459634288 1106 4 0.11082124320364528 1106 5 -0.18644585512686973 1106 6 0.06118622911795768 1106 7 0.2798433757196932 1106 8 0.00882289363361366 1106 9 -0.2256466185450705 1106 10-11 0.14401184130537104 1106 12-13 0.05681287840524618 1106 14-15 0.14756985951871826 1106 16-17 -0.24044747478350104 1106 18-19 -0.05599268321770978 1106 20-21 0.16054638803511523 1106 22-23 -0.08859833977018639 1106 24-25 0.2551704131121326 1106 26-27 -0.17540932899935768 1106 28-29 -0.07870345629156361 1106 30-31 -0.3795873626467454 1106 32-33 0.014606897955232512 1106 34-35 -0.2325384672922155 1106 36-37 0.4196969418277945 1106 38-39 0.13556825082328317 1106 40-41 -0.5260681460227516 1106 42-43 -0.6576405003180668 1106 44-45 -0.6961808028023526 1106 46-47 -0.4349524329072949 1106 48-49 -0.1540868829233304 1106 50-51 -0.35019606077360166 1106 52-53 -0.08723746311644476 1106 54-55 0.2519708388615882 1106 56-57 -0.43106577690267756 1106 58-59 -0.17475229737553022 1106 60-61 0.7614496397426755 1106 62-63 0.2430509427326264 1106 64-65 0.09384793745648068 1106 66-67 0.1072823879550242 1106 68-69 -0.3529700191822336 1106 70-71 -0.21004966907591793 1106 72-73 0.1866295819661552 1106 74-75 -0.39572897653716055 1106 76-77 -0.7495641077738924 1106 78-79 -0.5125498100518122 1106 80-81 -0.7658852555257276 1106 82-83 -0.8260448467567656 1106 84-85 -0.33399037610256244 1106 86-87 -0.15868746719694826 1106 88-89 -1.5237693365365956 1106 90-91 -1.4625089924120012 1106 92-93 -0.9500217042711263 1106 94-95 -1.288078164966695 1106 96-97 -1.8540526361408851 1106 98-99 -1.8224356923268132 1106 100-101 -0.8616106745114909 1107 1 0.2718986738257776 1107 2 0.05531091229440932 1107 3 0.048578092428609665 1107 4 0.44615993137638554 1107 5 -0.1700561442231603 1107 6 0.3249174898899696 1107 7 0.27870520135138577 1107 8 0.5274076551047813 1107 9 0.35046472742742196 1107 10-11 0.5640632217482775 1107 12-13 0.26791170445968504 1107 14-15 0.3999031169737606 1107 16-17 0.3557445189728057 1107 18-19 0.44856001425554837 1107 20-21 0.3284433669951454 1107 22-23 0.5995418833119857 1107 24-25 0.8347125418234 1107 26-27 0.602451553573566 1107 28-29 0.7158723298617318 1107 30-31 0.7167834870817202 1107 32-33 0.4991602458138331 1107 34-35 0.24726706748714378 1107 36-37 0.7861728287908178 1107 38-39 0.5981385737396181 1107 40-41 0.2282000078584332 1107 42-43 -0.019043381525186476 1107 44-45 -0.02696679350184894 1107 46-47 -0.6082963892013993 1107 48-49 -0.30739897033512165 1107 50-51 -0.4779480037998489 1107 52-53 -0.3729192295630632 1107 54-55 -0.5288818163366145 1107 56-57 -0.8493773765254531 1107 58-59 -0.6366884945723648 1107 60-61 -1.0345895134555931 1107 62-63 -0.39276577867481066 1107 64-65 -0.48349914068732147 1107 66-67 -0.1936834342946554 1107 68-69 -0.4385282124117218 1107 70-71 -0.45186294344098243 1107 72-73 -0.5772154962426832 1107 74-75 -1.3508198678902872 1107 76-77 -0.9528095306869666 1107 78-79 -0.6962674127314017 1107 80-81 -0.5333237128116686 1107 82-83 -0.6554812878481115 1107 84-85 -1.155183182840556 1107 86-87 -0.8624694580265171 1107 88-89 -0.8025895375056677 1107 90-91 -0.5592538137186231 1107 92-93 -0.9586718294703047 1107 94-95 -1.351637073620072 1107 96-97 -1.3849622000575046 1107 98-99 -1.3483535486566858 1107 100-101 -1.8651877939547603 1108 1 -0.8308633734014883 1108 2 -0.28927718472450437 1108 3 -0.2071383302661225 1108 4 0.015878918447654655 1108 5 -0.21373068210114354 1108 6 -0.48251385961318505 1108 7 -0.41957987184019885 1108 8 -0.6932622882652382 1108 9 -0.9346084641528734 1108 10-11 -0.6700964106910803 1108 12-13 -0.32861303108898454 1108 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-0.6041506822812721 2316 66-67 -0.6888666803679584 2316 68-69 -0.43546829385442365 2316 70-71 -0.45848307142236777 2316 72-73 -0.6365174159634499 2316 74-75 -0.5142520959298444 2316 76-77 -0.12800441143158636 2316 78-79 -0.47444076933407686 2316 80-81 -0.6019101002462257 2316 82-83 -0.7112346328161152 2316 84-85 -0.23225124566778277 2316 86-87 0.022789413410365 2316 88-89 -0.7144801715952553 2316 90-91 -0.20733991028639664 2316 92-93 -0.4584275013725758 2316 94-95 -0.7827365500598624 2316 96-97 -1.2475101930766996 2316 98-99 -1.3741127109603504 2316 100-101 -0.30216277941142167 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4351.0 3 653.0 4 470.0 5 403.0 6 402.0 7 406.0 8 465.0 9 448.0 10 552.0 11 752.0 12 1078.0 13 1504.0 14 1775.0 15 2438.0 16 2812.0 17 3629.0 18 3246.0 19 2239.0 20 2382.0 21 2206.0 22 2359.0 23 2338.0 24 2458.0 25 2451.0 26 2774.0 27 2978.0 28 3456.0 29 3958.0 30 4809.0 31 5687.0 32 7085.0 33 9044.0 34 12643.0 35 18993.0 36 21121.0 37 28944.0 38 19895.0 39 1511.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.54441661516442 23.950167517808932 16.48884840993614 18.016567457090503 2 15.111939287900372 25.88335346597403 38.583167667881845 20.421539578243745 3 18.40455531453362 30.864425162689805 32.33405639913232 18.39696312364425 4 11.547052381623603 22.202283496156223 39.16532035087339 27.085343771346786 5 9.864366319444445 41.057942708333336 35.804036458333336 13.27365451388889 6 24.155048011718115 41.87001573265339 17.631964411653016 16.34297184397548 7 21.10703748698369 38.78698802499132 24.49019437695245 15.615780111072544 8 23.085300045510696 34.98363782155473 21.94590728821273 19.98515484472184 9 24.039769924265833 20.086630778321705 23.910745360807976 31.962853936604485 10-11 21.54217193957049 30.50606327484588 27.73904207032044 20.212722715263194 12-13 24.387942674019488 30.051675803469273 28.34848903854809 17.211892483963148 14-15 21.610213144353654 31.629244858455014 28.98351126991959 17.777030727271743 16-17 16.559901756402507 34.25920152678792 30.530336506008744 18.650560210800833 18-19 16.07495737361854 32.58553061373857 34.36865517493759 16.970856837705306 20-21 17.70779681968262 31.08128622314269 34.21064045498165 17.000276502193042 22-23 17.68152265015339 30.633108472820087 34.73999994575138 16.94536893127514 24-25 17.92156703156581 31.721808488991982 34.09948588559259 16.257138593849618 26-27 16.708013068174115 31.229953773367498 35.49988477232367 16.56214838613472 28-29 16.36542537908153 31.777522376246214 35.4783447812251 16.378707463447164 30-31 17.16014491379147 31.80272431424724 34.37394153048257 16.663189241478726 32-33 16.312496950707164 32.44962568236397 35.07527009958313 16.162607267345734 34-35 16.984449977917286 31.619462048896267 34.56906198603505 16.827025987151384 36-37 16.545425482052238 31.33061861451806 34.73431247443098 17.389643428998724 38-39 16.136816674972373 31.926418651008603 35.081413992589866 16.855350681429158 40-41 15.830602128985074 32.01603510387605 35.120669573932126 17.03269319320675 42-43 16.16045333022734 31.530775044084308 33.849704343963225 18.459067281725126 44-45 16.381292295027365 31.076064027122978 34.30341663936136 18.239227038488306 46-47 17.297666217983963 31.49021339060583 34.18962215860618 17.022498232804033 48-49 16.85654322659265 31.28021122469704 33.814095186514116 18.049150362196194 50-51 15.911011440457617 31.3093148725949 34.23930707228289 18.540366614664585 52-53 16.422028533443804 31.985454387917283 33.31275502882317 18.279762049815744 54-55 16.86256879143736 30.853230489231702 33.19430601897994 19.089894700351 56-57 17.465045000162455 30.75742150693685 32.389286603922756 19.38824688897794 58-59 17.548202576762552 29.966984721970192 33.31293012623807 19.171882575029183 60-61 17.396367023754074 31.146218086892475 32.71736657965143 18.74004830970202 62-63 16.770987094240482 30.84029630432133 33.40221821973647 18.98649838170172 64-65 16.953903148407925 30.795415770745212 32.66994524272476 19.580735838122113 66-67 17.092457091482206 30.486034760096835 31.828669226644713 20.59283892177624 68-69 16.79194939932009 29.65042731569894 32.91292511478608 20.6446981701949 70-71 17.909687764078786 29.14969339775953 32.672639812788454 20.267979025373233 72-73 17.10610775718476 28.57274408955119 33.46794179064458 20.853206362619474 74-75 16.72683350891399 29.87100915095694 32.43945175503926 20.962705585089818 76-77 16.934802620891322 28.668706341040775 33.07494449558672 21.321546542481183 78-79 17.069010332055363 28.50548010483679 33.246420603461345 21.1790889596465 80-81 17.935825894368048 28.753800708833886 32.080013212793666 21.230360184004397 82-83 17.84479514399348 28.125363807914837 32.5376261990432 21.492214849048484 84-85 17.13495922632907 28.001169603309545 32.75593194641484 22.107939223946545 86-87 17.522364943898104 28.2935493653338 32.470053719187284 21.714031971580816 88-89 18.00106726765935 27.77171307294531 32.015591859510735 22.211627799884607 90-91 18.362413342656282 26.78911166398361 32.31431343534373 22.53416155801638 92-93 19.135504776560985 27.309032387413506 32.079632830661026 21.47583000536449 94-95 18.485236788422412 26.277134969807143 32.598807456512255 22.63882078525819 96-97 18.347234600475144 26.44825559188516 32.457991445235145 22.746518362404544 98-99 18.631163259336304 26.84288360906166 31.777553872655766 22.74839925894627 100-101 18.8877684893038 25.352383740472085 32.339340120786304 23.420507649437813 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3335.0 1 1903.5 2 544.0 3 616.5 4 463.5 5 388.5 6 690.0 7 1194.0 8 1531.0 9 1519.5 10 1157.5 11 754.0 12 641.0 13 626.0 14 597.0 15 603.0 16 653.5 17 697.5 18 709.5 19 729.5 20 810.0 21 888.5 22 957.5 23 1088.0 24 1225.0 25 1358.0 26 1552.0 27 1800.0 28 1954.0 29 2293.0 30 2994.5 31 3571.5 32 4375.0 33 5455.5 34 6027.0 35 6818.0 36 8719.0 37 10036.5 38 9588.5 39 8716.0 40 8045.5 41 7125.5 42 5875.0 43 5375.0 44 5655.0 45 5728.5 46 5310.5 47 5642.5 48 7156.5 49 7143.5 50 5373.0 51 3767.5 52 2727.5 53 1966.0 54 1449.5 55 1136.5 56 959.5 57 860.0 58 771.5 59 677.5 60 585.5 61 545.0 62 461.5 63 369.0 64 332.5 65 294.5 66 244.0 67 225.5 68 190.5 69 146.0 70 135.0 71 121.5 72 92.0 73 81.0 74 57.0 75 31.5 76 33.5 77 30.0 78 20.0 79 17.0 80 13.0 81 10.5 82 8.5 83 4.0 84 2.5 85 2.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13913325934547818 2 0.057385702298134965 3 0.17053298324445768 4 0.1412987575454078 5 0.2138429472430501 6 0.20842920174322607 7 0.17919497604417617 8 0.07741656064748396 9 0.13750913569553094 10-11 0.10962834637143706 12-13 0.16024686679479197 14-15 0.15483312129496793 16-17 0.14914868852015267 18-19 0.1426521939203638 20-21 0.14508837939528463 22-23 0.20491026716834043 24-25 0.22602387461765425 26-27 0.1610589286197656 28-29 0.13859188479549575 30-31 0.10367322632163062 32-33 0.1331781392956717 34-35 0.09825948082180656 36-37 0.08959748802208808 38-39 0.055761578648187746 40-41 0.06496494599788864 42-43 0.06794250602279187 44-45 0.0403324039736892 46-47 0.054408142273231735 48-49 0.041956527623636414 50-51 0.05792707684811737 52-53 0.02950491297404109 54-55 0.05359608044825813 56-57 0.02652735294913786 58-59 0.056032265923178955 60-61 0.03952034214871559 62-63 0.05819776412310857 64-65 0.04439271309855724 66-67 0.042227214898627616 68-69 0.07173212787266871 70-71 0.060092575048047 72-73 0.05711501502314376 74-75 0.04845302222342528 76-77 0.02436185474920824 78-79 0.02544460384917305 80-81 0.02463254202419944 82-83 0.019760171074357794 84-85 0.020030858349348996 86-87 0.027610102049102674 88-89 0.07119075332268629 90-91 0.12126789919605879 92-93 0.0909509243970441 94-95 0.08201824432233441 96-97 0.07552174972254555 98-99 0.06117532414801181 100-101 0.13913325934547818 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 184715.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 39.04988766478088 #Duplication Level Percentage of deduplicated Percentage of total 1 80.99430203379961 31.628183959072082 2 7.918925288710818 6.1846628589989985 3 3.1789383205556554 3.7241155293289663 4 1.6553215677032065 2.585604850715968 5 1.0328430217243625 2.0166201986844596 6 0.7625015596622812 1.7865360149419376 7 0.5808875518154468 1.587851555098395 8 0.4089781092734054 1.2776439379584765 9 0.3410461521398566 1.1986032536610454 >10 2.6798463905948897 21.486614514251684 >50 0.3091597232812522 8.231600032482474 >100 0.12061388307385175 8.38643315377744 >500 0.008318198832679431 2.2169287821779498 >1k 0.008318198832679431 7.6886013588501205 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3707 2.0068754567847766 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3117 1.6874644722951575 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2770 1.4996075034512628 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1805 0.9771810627182416 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1466 0.7936550902742062 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1337 0.7238177733264759 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 932 0.5045610805836017 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 746 0.4038654142868744 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 687 0.37192431583791247 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 641 0.3470210865387218 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 548 0.2966732533903581 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 541 0.29288363154048125 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 469 0.2539046639417481 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 444 0.240370300192188 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 443 0.23982892564220556 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 427 0.23116693284248707 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 407 0.22033944184283896 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 373 0.2019327071434372 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 368 0.19922583439352515 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 336 0.1819018487940882 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 302 0.16349511409468642 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 292 0.15808136859486235 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 275 0.14887800124516148 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 268 0.14508837939528463 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 258 0.1396746338954606 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 242 0.1310126410957421 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 241 0.1304712665457597 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 239 0.12938851744579488 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 237 0.12830576834583005 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 230 0.12451614649595323 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 226 0.12235064829602361 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 211 0.11423003004628753 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 209 0.1131472809463227 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 207 0.11206453184635791 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 198 0.10719216089651626 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 190 0.10286116449665703 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 188 0.1017784153966922 No Hit CTCATGAGCAGTCCCCTCCCTAGGACTTAAAACTGATGCCATCCCAGGCC 188 0.1017784153966922 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 187 0.10123704084670979 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 187 0.10123704084670979 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.7068727499120264E-4 0.0 20-21 0.0 0.0 0.0 0.0013534363749560132 0.0 22-23 0.0 0.0 0.0 0.004060309124868039 0.0 24-25 0.0 0.0 0.0 0.015158487399507349 0.0 26-27 0.0 0.0 0.0 0.04249790217361882 0.0 28-29 0.0 0.0 0.0 0.11450071732127873 0.0 30-31 0.0 0.0 0.0 0.28205614054083317 0.0 32-33 0.0 0.0 0.0 0.5256746880329155 0.0 34-35 0.0 0.0 0.0 0.9124868039953442 0.0 36-37 0.0 0.0 0.0 1.3970170262295971 0.0 38-39 0.0 0.0 0.0 1.9524673145115448 0.0 40-41 0.0 0.0 0.0 2.4494491513953927 0.0 42-43 0.0 0.0 0.0 2.9393931191294698 0.0 44-45 0.0 0.0 0.0 3.458300625287605 0.0 46-47 0.0 0.0 0.0 4.03567658284384 0.0 48-49 0.0 0.0 0.0 4.55323065262702 0.0 50-51 0.0 0.0 0.0 5.022331700186774 0.0 52-53 0.0 0.0 0.0 5.469507078472241 0.0 54-55 0.0 0.0 0.0 5.965135478981133 0.0 56-57 0.0 0.0 0.0 6.564437105811656 0.0 58-59 0.0 0.0 0.0 7.1071650921690175 0.0 60-61 0.0 0.0 0.0 7.622282976477276 0.0 62-63 0.0 0.0 0.0 8.09815120591181 0.0 64-65 0.0 0.0 0.0 8.597027853720597 0.0 66-67 0.0 0.0 0.0 9.161681509352245 0.0 68-69 0.0 0.0 0.0 9.764502070757654 0.0 70-71 0.0 0.0 0.0 10.400346479711988 0.0 72-73 0.0 0.0 0.0 10.96121051349376 0.0 74-75 0.0 0.0 0.0 11.558075954849363 0.0 76-77 0.0 0.0 0.0 12.199875483853504 0.0 78-79 0.0 0.0 0.0 12.915572638930243 0.0 80-81 0.0 0.0 0.0 13.614216495682538 0.0 82-83 0.0 0.0 0.0 14.260076333811547 0.0 84-85 0.0 0.0 0.0 14.932734212164686 0.0 86-87 0.0 0.0 0.0 15.57020274476897 0.0 88-89 0.0 0.0 0.0 16.289960208970577 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAAGCG 20 0.002059814 71.34999 5 GTGTAAG 25 0.00496009 57.157585 1 TGTAAGG 25 0.0049868436 57.07999 2 CGCAGAA 70 7.819472E-8 47.540844 9 GGTTGTG 40 5.3536036E-4 47.540844 7 AGTACCA 70 4.249321E-6 40.771423 2 CGTTGAA 50 0.0016055029 38.032673 9 GCCCGCA 25 0.001612956 38.00689 92-93 GTACCAC 80 1.0624453E-5 35.674995 3 GTAAGCG 60 1.4951729E-7 31.680996 94-95 ACACTGA 125 2.1162487E-7 30.434397 6 ATGGGGT 65 0.00580707 29.255903 7 GGATGAC 65 0.00580707 29.255903 8 CACTGAC 130 2.990946E-7 29.255903 7 GAGTACC 120 5.23587E-6 27.784937 1 AAGAGAG 70 0.008338584 27.166195 7 ACCATAC 105 6.8348265E-5 27.166195 8 CTCGCGC 35 0.008365823 27.155142 94-95 CATGTAG 110 9.344806E-5 25.94545 3 GCCATTG 110 9.379388E-5 25.931368 7 >>END_MODULE