##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938056_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 184715 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39016322442682 33.0 31.0 34.0 31.0 34.0 2 32.605094334515336 34.0 31.0 34.0 31.0 34.0 3 32.618623284519394 34.0 31.0 34.0 31.0 34.0 4 36.125149554719435 37.0 35.0 37.0 35.0 37.0 5 36.11130660747638 37.0 35.0 37.0 35.0 37.0 6 36.17666134315026 37.0 36.0 37.0 35.0 37.0 7 36.133069864385675 37.0 36.0 37.0 35.0 37.0 8 36.15415640310749 37.0 36.0 37.0 35.0 37.0 9 37.778853910077686 39.0 38.0 39.0 35.0 39.0 10-11 37.78637630944969 39.0 38.0 39.0 35.0 39.0 12-13 37.7414774111469 39.0 38.0 39.0 35.0 39.0 14-15 38.89254527244674 40.0 38.0 41.0 35.5 41.0 16-17 38.77586281568904 40.0 38.0 41.0 34.5 41.0 18-19 39.030314267926265 40.0 38.5 41.0 35.5 41.0 20-21 39.05621633327017 40.0 38.5 41.0 35.0 41.0 22-23 38.93447202447013 40.0 38.0 41.0 35.0 41.0 24-25 38.79929891995778 40.0 38.0 41.0 35.0 41.0 26-27 38.5706926887367 40.0 38.0 41.0 35.0 41.0 28-29 38.387889451316894 40.0 37.5 41.0 34.5 41.0 30-31 38.06011423003005 40.0 37.0 41.0 34.0 41.0 32-33 37.68132257802561 40.0 35.5 41.0 33.5 41.0 34-35 37.39959396908751 40.0 35.0 41.0 33.0 41.0 36-37 37.24271986573911 40.0 35.0 41.0 33.0 41.0 38-39 36.99424248166093 40.0 35.0 41.0 33.0 41.0 40-41 36.814286874374034 40.0 35.0 41.0 33.0 41.0 42-43 36.483461007498036 40.0 35.0 41.0 31.5 41.0 44-45 36.07040576022521 39.5 35.0 41.0 30.5 41.0 46-47 35.506266410416046 39.0 35.0 41.0 28.5 41.0 48-49 34.96216333270173 39.0 35.0 41.0 24.5 41.0 50-51 34.49466475380992 39.0 35.0 40.5 22.0 41.0 52-53 34.03821292261051 38.0 35.0 40.0 17.0 41.0 54-55 33.71415694448204 38.0 34.0 40.0 9.5 41.0 56-57 33.284576239071 38.0 34.0 40.0 2.0 41.0 58-59 32.72030425249709 38.0 33.0 40.0 2.0 41.0 60-61 32.083571989280784 37.0 32.5 40.0 2.0 41.0 62-63 31.568483880572774 37.0 31.5 40.0 2.0 41.0 64-65 30.978696911458194 36.0 31.0 39.5 2.0 41.0 66-67 30.293002733941478 36.0 30.0 39.0 2.0 40.0 68-69 29.65741818477113 35.0 29.0 39.0 2.0 40.0 70-71 29.09317597379747 35.0 28.0 38.0 2.0 40.0 72-73 28.558284384051106 35.0 27.0 37.0 2.0 39.5 74-75 28.0646617762499 34.0 26.0 37.0 2.0 39.0 76-77 26.734545110034375 32.5 25.0 35.0 2.0 37.5 78-79 27.354037300706494 34.0 26.0 36.0 2.0 38.0 80-81 27.059421270606066 34.0 25.5 35.5 2.0 37.0 82-83 26.603286143518392 34.0 24.5 35.0 2.0 37.0 84-85 26.11098719649189 33.0 22.0 35.0 2.0 36.0 86-87 25.660127764393796 33.0 19.5 35.0 2.0 36.0 88-89 25.078220501854208 32.5 17.0 35.0 2.0 35.5 90-91 24.70370570879463 32.0 9.0 35.0 2.0 35.0 92-93 24.077465284356983 32.0 2.0 34.5 2.0 35.0 94-95 23.50738705573451 31.0 2.0 34.0 2.0 35.0 96-97 22.748144438729938 31.0 2.0 34.0 2.0 35.0 98-99 21.665406166256126 30.5 2.0 34.0 2.0 35.0 100-101 17.77506699510056 23.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1370434148789812 1101 2 0.1244044628719223 1101 3 0.16536035363897383 1101 4 0.1331808568233157 1101 5 0.0760645741916548 1101 6 -0.08973325340959093 1101 7 0.1847654038433788 1101 8 0.2677442577126783 1101 9 0.05751265071591405 1101 10-11 0.005363573416921952 1101 12-13 0.2115725520623073 1101 14-15 0.2877041562080791 1101 16-17 0.44958863413928185 1101 18-19 0.14420702847592537 1101 20-21 0.2136105980216101 1101 22-23 0.36441657222194124 1101 24-25 0.1532704551462558 1101 26-27 0.26681448266038643 1101 28-29 0.273700837529411 1101 30-31 -0.02644466232879239 1101 32-33 0.1982648840031871 1101 34-35 0.25811697616224905 1101 36-37 0.38850425090576834 1101 38-39 0.2585338824642349 1101 40-41 0.43018997922970215 1101 42-43 -0.010548450362449557 1101 44-45 -0.01765053810241568 1101 46-47 -0.0663488642432668 1101 48-49 0.07223879197412231 1101 50-51 -0.640691467176687 1101 52-53 -0.512213750799198 1101 54-55 -0.24481850740384203 1101 56-57 -0.4342340778856695 1101 58-59 -0.2748831838687522 1101 60-61 -0.6128454693808791 1101 62-63 -0.11432029614127615 1101 64-65 -0.10486966582849178 1101 66-67 -0.8339283010981902 1101 68-69 -0.8124589468476024 1101 70-71 -0.8058733744521724 1101 72-73 0.019300491968820666 1101 74-75 -0.2518078904085783 1101 76-77 -0.20105907608624207 1101 78-79 0.22185523395415885 1101 80-81 -0.21957259045090538 1101 82-83 -0.40615351790668086 1101 84-85 -0.23682496662090813 1101 86-87 -0.9932981377470327 1101 88-89 -0.895029682351538 1101 90-91 -1.114674470296908 1101 92-93 -1.1442578753348265 1101 94-95 -1.132347548118048 1101 96-97 -0.9266725923373009 1101 98-99 -0.7793728886422855 1101 100-101 -0.4414475060225662 1102 1 0.250395509938393 1102 2 0.4170873897011873 1102 3 0.32780563193791323 1102 4 0.1630432708320697 1102 5 0.17612711327858221 1102 6 0.4293411681038535 1102 7 0.2901437653193 1102 8 0.10623706571767855 1102 9 0.20494854815181185 1102 10-11 0.1697631981824017 1102 12-13 0.32805160146818224 1102 14-15 0.15801372468838082 1102 16-17 0.2175686216314645 1102 18-19 0.4445509934540368 1102 20-21 0.33837607644561984 1102 22-23 0.3420588486447045 1102 24-25 0.5531297422006674 1102 26-27 0.5378432506403712 1102 28-29 0.4502955842461134 1102 30-31 0.5117041806981035 1102 32-33 0.7228896494816084 1102 34-35 0.36795124758189246 1102 36-37 0.7565467774848855 1102 38-39 0.870791543502385 1102 40-41 0.34857647078693077 1102 42-43 0.3113714996062811 1102 44-45 0.38830630992760007 1102 46-47 0.5485667079893872 1102 48-49 0.4890618063581158 1102 50-51 0.8206906466444437 1102 52-53 0.936582371777412 1102 54-55 0.2688619178619476 1102 56-57 0.42380219478474856 1102 58-59 0.44251831706934297 1102 60-61 0.4638430859662108 1102 62-63 1.0695446194309604 1102 64-65 0.45305716343979796 1102 66-67 0.8576758264815503 1102 68-69 0.5329913345782877 1102 70-71 0.4200490770800336 1102 72-73 0.29603595163110796 1102 74-75 0.7509281946445796 1102 76-77 0.9399665649394002 1102 78-79 1.0748883796702344 1102 80-81 1.298094701606633 1102 82-83 1.4548533613928818 1102 84-85 1.1617835386949125 1102 86-87 1.4078119310459627 1102 88-89 1.64906037393364 1102 90-91 1.2115450418982157 1102 92-93 0.9470491915819963 1102 94-95 0.6897287495680047 1102 96-97 0.9426988898390611 1102 98-99 0.8793982182995563 1102 100-101 -0.05691029526584401 1103 1 0.1631423159778791 1103 2 0.08273779620525801 1103 3 0.19191713019574763 1103 4 0.1739317725742353 1103 5 -0.1345581364310533 1103 6 0.19414953047319727 1103 7 0.2081170521950284 1103 8 0.06994205991048119 1103 9 0.43113902434228635 1103 10-11 0.40531778587113365 1103 12-13 0.3047502077399571 1103 14-15 0.5754788814828018 1103 16-17 0.38067837772902635 1103 18-19 0.6405440248129182 1103 20-21 0.4290410009520116 1103 22-23 0.5578689531743208 1103 24-25 0.4932429826187814 1103 26-27 0.4032613691072626 1103 28-29 0.4534169547455278 1103 30-31 0.6931066197224922 1103 32-33 0.6762868620251723 1103 34-35 0.7924576355029131 1103 36-37 0.808376045777564 1103 38-39 0.997315933746286 1103 40-41 0.983303532343264 1103 42-43 0.94000100018701 1103 44-45 1.292560084608212 1103 46-47 0.8995394141450177 1103 48-49 0.2450867773221077 1103 50-51 0.058713294728072185 1103 52-53 0.29524961916417425 1103 54-55 0.35110640102106316 1103 56-57 0.2537237975722064 1103 58-59 0.345538061552503 1103 60-61 0.40569848666307706 1103 62-63 -0.041518098339079046 1103 64-65 -0.17927442773325097 1103 66-67 -0.3375913047611938 1103 68-69 -0.04620436809302575 1103 70-71 -0.07029645137524909 1103 72-73 -0.4564321087637815 1103 74-75 -0.23440862300930831 1103 76-77 -0.38443819696535897 1103 78-79 -0.4933462312473047 1103 80-81 -0.20263119850951128 1103 82-83 -0.27039344464660786 1103 84-85 0.23226843997249702 1103 86-87 0.5002916058427083 1103 88-89 -0.38106448088633726 1103 90-91 -0.004555422677860577 1103 92-93 -0.14151062258757463 1103 94-95 -0.056569159839661154 1103 96-97 -0.08670006486477178 1103 98-99 0.6153157560463605 1103 100-101 0.9415653144902549 1104 1 -0.3119659091303433 1104 2 -0.0346864462189842 1104 3 -0.2317841685055484 1104 4 -0.04441238326992192 1104 5 0.008028443655526019 1104 6 -0.10779852147485514 1104 7 -0.26293273135475914 1104 8 0.01366500363342027 1104 9 -0.47686963366636803 1104 10-11 -0.013648664345318196 1104 12-13 -0.04265238966263496 1104 14-15 0.04098212823605252 1104 16-17 -0.30398071318006714 1104 18-19 -0.38908259856369654 1104 20-21 -0.04403800337698982 1104 22-23 0.12483323888860554 1104 24-25 0.012317657943455629 1104 26-27 0.12404058988648359 1104 28-29 -0.23394776386918892 1104 30-31 -0.22734792573205453 1104 32-33 -0.23726833278002601 1104 34-35 -0.5101668233715415 1104 36-37 -0.9813966814951698 1104 38-39 -0.43166133898098735 1104 40-41 -0.9360687620290307 1104 42-43 -1.0219849305489248 1104 44-45 -1.419966971668849 1104 46-47 -1.480465997110393 1104 48-49 -1.8033439586086288 1104 50-51 -1.7448581338433584 1104 52-53 -1.8516659652514136 1104 54-55 -2.3081197894551266 1104 56-57 -1.8160813972329848 1104 58-59 -1.809629687365252 1104 60-61 -1.836184630220039 1104 62-63 -1.867816799637783 1104 64-65 -1.9258923931012184 1104 66-67 -2.3435436857135734 1104 68-69 -2.293228177616836 1104 70-71 -1.805144936223737 1104 72-73 -1.586031675863346 1104 74-75 -1.4087863286370137 1104 76-77 -1.6537834350605998 1104 78-79 -1.2994880061390788 1104 80-81 -1.5022794669077797 1104 82-83 -1.6958534013565654 1104 84-85 -1.3778506076465504 1104 86-87 -1.6086099093088038 1104 88-89 -1.0934562557781113 1104 90-91 -1.3817199248423613 1104 92-93 -2.2071220944490477 1104 94-95 -1.9871698091903092 1104 96-97 -1.692625389540094 1104 98-99 -1.274565196334592 1104 100-101 -1.2345710491461084 1105 1 0.20532658098823475 1105 2 0.18962185417627353 1105 3 0.2563581239845689 1105 4 0.2725342570462814 1105 5 0.21973792982153384 1105 6 0.18172717022474671 1105 7 0.2200218140997876 1105 8 0.168803343554373 1105 9 0.35712246119528857 1105 10-11 0.38914283763096336 1105 12-13 0.12384958662195089 1105 14-15 0.37361552744553705 1105 16-17 0.5953522907725102 1105 18-19 0.4643017887880845 1105 20-21 0.36912899267043286 1105 22-23 0.4142354128016521 1105 24-25 0.5242988832399078 1105 26-27 0.3065998784240378 1105 28-29 0.45367575391737347 1105 30-31 0.4811500979833596 1105 32-33 0.41231170674566187 1105 34-35 0.3629804098300369 1105 36-37 0.6616369153427826 1105 38-39 0.8994898467897627 1105 40-41 0.7082000126745172 1105 42-43 0.8687018283443564 1105 44-45 0.35609528129558043 1105 46-47 0.43615949696074807 1105 48-49 0.6796105868459179 1105 50-51 0.49970708354794624 1105 52-53 0.5848458924560944 1105 54-55 0.6905215148927013 1105 56-57 0.6718138596840078 1105 58-59 0.9753258462315841 1105 60-61 0.27992208914755423 1105 62-63 -0.029872135606161976 1105 64-65 -0.001608796263273149 1105 66-67 0.23797805341686384 1105 68-69 0.1578587789049024 1105 70-71 0.33640644717547374 1105 72-73 0.70868176287183 1105 74-75 0.5379775053550802 1105 76-77 0.5867056953741816 1105 78-79 0.48349124287278045 1105 80-81 0.2676068967285836 1105 82-83 0.2689440294734773 1105 84-85 -0.13872017286394112 1105 86-87 -0.06130259705584251 1105 88-89 0.44903386279896296 1105 90-91 0.48043422535526403 1105 92-93 0.23840656167743646 1105 94-95 0.05173829357648785 1105 96-97 0.11283409662591382 1105 98-99 -0.47565215285633045 1105 100-101 -1.0312607724662683 1106 1 0.03296633254102943 1106 2 0.10732371753030634 1106 3 0.02266247181065495 1106 4 0.007006306735725332 1106 5 0.22517271243022918 1106 6 0.1615408348210181 1106 7 0.025146037702270974 1106 8 0.11134992740529981 1106 9 0.08072494566734179 1106 10-11 -0.020018653052268576 1106 12-13 -0.24144544443394977 1106 14-15 0.016860869060444372 1106 16-17 0.20870632803958244 1106 18-19 0.18712787574460066 1106 20-21 0.2161786821114262 1106 22-23 0.2267354128016521 1106 24-25 0.03866223727717255 1106 26-27 -0.002794531513849563 1106 28-29 0.38884656137078366 1106 30-31 0.4924078619585188 1106 32-33 0.3214731974288938 1106 34-35 0.6125922110722755 1106 36-37 0.9069785302496172 1106 38-39 0.7795364306406967 1106 40-41 0.7780757890720409 1106 42-43 1.2526303997729258 1106 44-45 0.9446045980657658 1106 46-47 0.6504452112464634 1106 48-49 1.0821726986471631 1106 50-51 0.8929524251628536 1106 52-53 0.3725011719592004 1106 54-55 0.5150556763833833 1106 56-57 0.8107890149635111 1106 58-59 0.5766457220079815 1106 60-61 0.3346581139922691 1106 62-63 0.9608110942075037 1106 64-65 0.7219936882087765 1106 66-67 0.21546252546655253 1106 68-69 0.33798300250738933 1106 70-71 0.04370458382143738 1106 72-73 0.3150482224991613 1106 74-75 0.1059122879637755 1106 76-77 0.1868609748772876 1106 78-79 0.5126061497050785 1106 80-81 0.1596876420701996 1106 82-83 0.2111024145666427 1106 84-85 0.036745665645376846 1106 86-87 -0.1304019759378292 1106 88-89 0.07015187522132393 1106 90-91 -0.07507819700498075 1106 92-93 0.09477302130476772 1106 94-95 0.019905995439842172 1106 96-97 -0.37280254933682144 1106 98-99 -0.2865993578252741 1106 100-101 -1.1286986606650231 1107 1 0.13549958349221924 1107 2 -0.0706217151385502 1107 3 -0.09024442552601641 1107 4 -0.13487525809143364 1107 5 -0.07553295567812768 1107 6 -0.39242488987085977 1107 7 0.049210295775182544 1107 8 -0.07391234048842676 1107 9 0.0217024748533845 1107 10-11 0.04669462456472928 1107 12-13 -0.016444631452259273 1107 14-15 -0.040779532877699864 1107 16-17 -0.016245706040606933 1107 18-19 0.07023736811883197 1107 20-21 0.13975840309611698 1107 22-23 -0.04745079252500517 1107 24-25 -0.2507267256833856 1107 26-27 -0.2952240460011879 1107 28-29 -0.46834507467357156 1107 30-31 -0.6743672022670353 1107 32-33 -0.3679352770922648 1107 34-35 0.1559891737726744 1107 36-37 -0.13599568197112433 1107 38-39 -0.31649296677727534 1107 40-41 -0.29982411662208364 1107 42-43 -0.22467115139613725 1107 44-45 0.03712191457579905 1107 46-47 0.3175617277825111 1107 48-49 -0.4188841775519876 1107 50-51 -0.696101589340735 1107 52-53 -0.46994297619858827 1107 54-55 -0.1171839255807825 1107 56-57 -0.5512967708645888 1107 58-59 -0.8551288544285569 1107 60-61 -0.5744342732471672 1107 62-63 -0.5479691080623432 1107 64-65 -1.0714927024777054 1107 66-67 -1.4037531097973428 1107 68-69 -1.1263433608629079 1107 70-71 -0.905484060450295 1107 72-73 -0.8534341032788646 1107 74-75 -0.504945895509934 1107 76-77 -1.4042742727569362 1107 78-79 -1.5730333416935771 1107 80-81 -1.5838328938473332 1107 82-83 -1.3399365760352886 1107 84-85 -1.0990547961282253 1107 86-87 -1.1190364918885294 1107 88-89 -1.5010002824320843 1107 90-91 -1.3522399153230857 1107 92-93 -0.5715467731734591 1107 94-95 -1.1606734979099684 1107 96-97 -0.9726854799732223 1107 98-99 -1.1544735732979419 1107 100-101 -1.4913535336772838 1108 1 -0.1846467996683785 1108 2 0.02236364654539358 1108 3 -0.1244094004164964 1108 4 -0.042904962119642676 1108 5 -0.020612558199765374 1108 6 -0.0609525103431352 1108 7 -0.2264067573287818 1108 8 -0.24978583124598686 1108 9 -0.15219430899104225 1108 10-11 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-0.10729463961649444 2316 90-91 -0.0018966925191286066 2316 92-93 -0.060796336873288226 2316 94-95 0.20132766555716586 2316 96-97 0.7390539033891947 2316 98-99 1.0146014703320745 2316 100-101 1.302398647823587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 3.0 5 11.0 6 21.0 7 43.0 8 119.0 9 152.0 10 326.0 11 534.0 12 837.0 13 1247.0 14 1447.0 15 2069.0 16 2885.0 17 3650.0 18 3677.0 19 2632.0 20 2417.0 21 2508.0 22 2763.0 23 2802.0 24 2943.0 25 3093.0 26 2595.0 27 2434.0 28 2660.0 29 3193.0 30 3831.0 31 4735.0 32 5912.0 33 7666.0 34 11152.0 35 18392.0 36 22324.0 37 35694.0 38 26262.0 39 1686.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.54130526589351 24.64509631939707 16.841005316787406 18.972593097922015 2 14.471817640370348 25.49786128106557 39.388163950403374 20.6421571281607 3 17.65349214943151 31.132106118029235 33.60530590146183 17.609095831077422 4 10.798856610760419 23.173663068311008 38.21096397674243 27.816516344186148 5 9.064824539558453 42.07260954773325 35.920395855214004 12.942170057494288 6 23.40266574271577 42.089392249639985 17.22500730859599 17.28293469904826 7 20.134260888395637 39.033105053731425 23.73819126762851 17.09444279024443 8 22.85358525295726 35.34905124110115 21.894269550388437 19.90309395555315 9 22.429688980321036 19.649189291611403 23.461007498037517 34.46011423003005 10-11 21.100073085564247 30.19137590341878 27.962266193866224 20.746284817150745 12-13 24.05983287722404 29.205611903775825 28.32019143055905 18.41436378844108 14-15 21.341796822131393 31.408656579054217 28.106001136886555 19.143545461927836 16-17 16.63869458821491 34.44084717500054 29.769149143513 19.151309093271543 18-19 15.91289283490783 31.988739409360367 33.76796686787754 18.33040088785426 20-21 17.3353544649866 30.49941802235877 33.8215629483258 18.343664564328833 22-23 17.251170722464337 30.99288092466773 34.23436104268738 17.521587310180546 24-25 17.88953946353308 32.24323731717815 33.689841019768984 16.177382199519784 26-27 16.764719419318332 31.49510367961793 35.32931375707645 16.410863143987285 28-29 15.441795497180664 31.42715605568835 35.596473323894934 17.534575123236053 30-31 16.85461996979032 31.07184815033377 35.30824161248234 16.765290267393578 32-33 15.773218201012371 32.70416587716212 35.164171832282165 16.35844408954335 34-35 16.10286951813752 32.093936112615054 35.50649702219816 16.296697347049268 36-37 16.422956570698485 31.353335426659594 34.80840650952283 17.41530149311909 38-39 15.624264047057675 31.26991302558883 35.61728010741191 17.488542819941582 40-41 15.300846723548009 31.3907596691065 35.93134176447659 17.377051842868898 42-43 16.05891381377886 30.741349300662392 34.97202378857726 18.227713096981493 44-45 15.792978372086727 30.61039980510516 35.04236255853612 18.554259264271984 46-47 17.543289779632893 30.470266275793183 34.3952423875765 17.591201556997422 48-49 16.50772683101127 30.153183172859592 34.46986820129552 18.86922179483362 50-51 15.174861331810172 30.606620881575065 35.220231343748225 18.99828644286654 52-53 15.702018451942651 31.45087061982934 34.67535842682029 18.171752501407717 54-55 16.084128114264367 30.486289521344705 33.63581481852464 19.79376754586628 56-57 17.33782759367555 30.83576998050682 32.11176088369071 19.714641542126923 58-59 16.510259006752694 31.252470663410353 33.474037050474635 18.76323327936231 60-61 16.885707866263505 33.236242362883814 31.527314956430878 18.3507348144218 62-63 15.462340496386886 33.63702081229406 32.01335322418634 18.88728546713271 64-65 15.897331762985825 33.4589611338992 31.321664026858357 19.322043076256616 66-67 16.136944468509054 32.88437797799532 30.304892575586933 20.67378497790869 68-69 15.973463308962904 31.840509071215813 31.386406715407528 20.799620904413757 70-71 17.281467182512504 31.052276769880955 31.076101699962912 20.590154347643633 72-73 16.21892325017325 30.41460932085932 31.988208160083158 21.37825926888427 74-75 16.11454345601334 31.58202522186906 30.57028064608704 21.73315067603056 76-77 15.992960606470827 30.19087586300257 31.975091376742924 21.841072153783674 78-79 16.160877711711127 29.94579285397104 31.978154682609304 21.915174751708527 80-81 16.9907196846642 30.00373594958092 30.949905789097524 22.055638576657355 82-83 16.79303733711723 29.34597762155956 31.505291320501293 22.355693720821915 84-85 16.036800979049 29.389610600588078 31.679924621891058 22.89366379847186 86-87 16.275700719471214 29.871729953235725 31.13249697399668 22.72007235329638 88-89 16.563237810557325 29.344011956592368 30.383986397210126 23.708763835640177 90-91 16.606917540790963 28.215486756813835 31.034436074753195 24.143159627642003 92-93 17.285260957620785 28.931963653298386 30.76879338914581 23.01398199993502 94-95 16.53599584163562 27.793359611885993 31.601403447977127 24.069241098501255 96-97 16.200696856565045 28.427903501338754 31.338782206604165 24.03261743549204 98-99 16.48036903985208 28.78494603279452 30.556781296783083 24.177903630570317 100-101 16.241195038360125 28.000725512596308 30.805048268244743 24.95303118079882 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3155.0 1 1783.5 2 493.5 3 649.5 4 492.0 5 337.0 6 677.0 7 1202.5 8 1518.5 9 1552.5 10 1179.0 11 726.5 12 607.0 13 601.5 14 623.5 15 617.5 16 593.0 17 572.5 18 606.0 19 656.5 20 700.5 21 767.5 22 839.5 23 925.0 24 1040.0 25 1209.0 26 1456.0 27 1739.0 28 1893.5 29 2224.0 30 2927.5 31 3524.0 32 4427.0 33 5542.0 34 6096.0 35 6965.5 36 8886.5 37 10064.5 38 9641.0 39 8812.0 40 8131.5 41 7316.5 42 6192.5 43 5956.0 44 6437.0 45 6543.0 46 5974.5 47 6200.5 48 7745.0 49 7469.0 50 5885.5 51 4434.0 52 2706.0 53 1528.0 54 1037.5 55 821.5 56 689.0 57 597.5 58 515.0 59 418.0 60 328.5 61 287.0 62 229.0 63 166.5 64 98.5 65 65.0 66 51.5 67 43.5 68 32.5 69 16.0 70 12.5 71 9.0 72 6.0 73 7.5 74 6.5 75 4.5 76 3.0 77 2.0 78 1.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00920336734970089 2 0.013534363749560132 3 0.00812061824973608 4 5.413745499824053E-4 5 5.413745499824053E-4 6 5.413745499824053E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 2.7068727499120264E-4 14-15 0.0 16-17 0.0037896218498768373 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.003518934574885635 26-27 0.01921879652437539 28-29 0.004601683674850445 30-31 0.003248247299894432 32-33 0.0 34-35 0.00812061824973608 36-37 5.413745499824053E-4 38-39 0.0029775600249032294 40-41 0.001624123649947216 42-43 0.002436185474920824 44-45 0.0 46-47 2.7068727499120264E-4 48-49 8.12061824973608E-4 50-51 0.006225807324797661 52-53 0.0102861164496657 54-55 0.01109817827463931 56-57 0.018948109249384188 58-59 0.025985978399155455 60-61 0.004601683674850445 62-63 0.021925669274287417 64-65 0.023008418374252226 66-67 0.014617112849524945 68-69 0.03518934574885635 70-71 0.018677421974392986 72-73 0.005955120049806459 74-75 0.01840673469940178 76-77 0.021654981999296215 78-79 0.028692851149067482 80-81 0.012451614649595322 82-83 0.03952034214871559 84-85 0.024903229299190643 86-87 0.03546003302384755 88-89 0.02544460384917305 90-91 0.02923422569904989 92-93 0.02652735294913786 94-95 0.014617112849524945 96-97 0.015429174674498551 98-99 0.010556803724656904 100-101 0.009744741899683296 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 184715.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.03134558644398 #Duplication Level Percentage of deduplicated Percentage of total 1 77.71273126421788 26.44668814119048 2 9.245796280682777 6.29293776899548 3 3.6397766500692 3.7159949110792305 4 2.0251030050428724 2.756679208510408 5 1.1962902276451217 2.0355683079338442 6 0.9369879575571499 1.9132176596378205 7 0.6426878350646665 1.5310072273502424 8 0.5042872369195527 1.3729258587553799 9 0.40088449117895036 1.2278374793600952 >10 3.1148088640015272 21.48553176515172 >50 0.3961120567601534 9.089678694204585 >100 0.15908114729323428 9.565546923639118 >500 0.0111356803105264 2.5574533741168826 >1k 0.014317303256391084 10.00893268007471 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3468 1.8774869393389817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3009 1.6289960208970575 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2901 1.5705275694989578 No Hit CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT 2227 1.2056411228108168 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1837 0.9945050483176785 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1514 0.8196410686733617 No Hit ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT 1337 0.7238177733264759 TruSeq Adapter, Index 11 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1157 0.6263703543296429 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1038 0.5619467828817367 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 837 0.45313049833527325 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 750 0.406030912486804 No Hit ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT 712 0.3854586795874726 RNA PCR Primer, Index 11 (95% over 23bp) GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 674 0.3648864466881412 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 648 0.3508107083885987 No Hit TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG 593 0.3210351081395663 TruSeq Adapter, Index 11 (96% over 28bp) GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 510 0.2761010204910267 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 496 0.26852177679127304 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 442 0.23928755109222316 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 434 0.23495655469236393 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 430 0.23279105649243428 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 412 0.22304631459275098 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 389 0.21059469994315566 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 348 0.18839834339387704 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 335 0.18136047424410578 No Hit CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCT 334 0.18081909969412338 TruSeq Adapter, Index 11 (96% over 26bp) GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 320 0.17323985599436972 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.16728473594456325 No Hit TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC 307 0.16620198684459844 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 284 0.15375037219500312 No Hit TTCTAGGACAATGGGCATAAAGCTATGGTTAGATCCACAAATTTCAGAGC 280 0.1515848739950735 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 273 0.14779525214519665 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 268 0.14508837939528463 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 245 0.1326367647456893 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 238 0.12884714289581248 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 235 0.12722301924586527 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 235 0.12722301924586527 No Hit GAATAACCCTGGTCGGTTTGATGTTACTGTTGCTTGATTTAGTCGGCCTG 231 0.12505752104593565 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 230 0.12451614649595323 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 222 0.120185150096094 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 215 0.11639552824621714 No Hit CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTG 212 0.11477140459626993 TruSeq Adapter, Index 11 (95% over 24bp) GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 208 0.11260590639634031 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 204 0.1104404081964107 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 196 0.10610941179655144 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 195 0.10556803724656905 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 192 0.10394391359662182 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 185 0.10015429174674499 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 2.7068727499120264E-4 0.0 20-21 0.0 0.0 0.0 0.0013534363749560132 0.0 22-23 0.0 0.0 0.0 0.004601683674850445 0.0 24-25 0.0 0.0 0.0 0.01624123649947216 0.0 26-27 0.0 0.0 0.0 0.043580651273583626 0.0 28-29 0.0 0.0 0.0 0.11450071732127873 0.0 30-31 0.0 0.0 0.0 0.284492326015754 0.0 32-33 0.0 0.0 0.0 0.5297349971577836 0.0 34-35 0.0 0.0 0.0 0.9176298622201771 0.0 36-37 0.0 0.0 0.0 1.4056790190293156 0.0 38-39 0.0 0.0 0.0 1.960858620036272 0.0 40-41 0.0 0.0 0.0 2.4610887042200145 0.0 42-43 0.0 0.0 0.0 2.952656795604039 0.0 44-45 0.0 0.0 0.0 3.4718349890371654 0.0 46-47 0.0 0.0 0.0 4.051917819343313 0.0 48-49 0.0 0.0 0.0 4.568930514576509 0.0 50-51 0.0 0.0 0.0 5.042903933086105 0.0 52-53 0.0 0.0 0.0 5.495763744146387 0.0 54-55 0.0 0.0 0.0 5.994099017405192 0.0 56-57 0.0 0.0 0.0 6.598814389735539 0.0 58-59 0.0 0.0 0.0 7.141271688817909 0.0 60-61 0.0 0.0 0.0 7.6599085077010525 0.0 62-63 0.0 0.0 0.0 8.141731857185395 0.0 64-65 0.0 0.0 0.0 8.642503315919118 0.0 66-67 0.0 0.0 0.0 9.213924153425548 0.0 68-69 0.0 0.0 0.0 9.819180900305877 0.0 70-71 0.0 0.0 0.0 10.462875240234958 0.0 72-73 0.0 0.0 0.0 11.029423706791544 0.0 74-75 0.0 0.0 0.0 11.63657526459681 0.0 76-77 0.0 0.0 0.0 12.283788539100776 0.0 78-79 0.0 0.0 0.0 13.010042497902173 0.0 80-81 0.0 0.0 0.0 13.717889722004168 0.0 82-83 0.0 0.0 0.0 14.375930487507782 0.0 84-85 0.0 0.0 0.0 15.06185204233549 0.0 86-87 0.0 0.0 0.0 15.716373873264217 0.0 88-89 0.0 0.0 0.0 16.458057006740113 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGTCC 40 5.3798454E-4 47.493774 6 GCTTTGC 55 4.9509053E-5 43.17616 1 AGGGGTC 45 9.6102705E-4 42.216686 5 CTCCGAC 60 8.731149E-11 39.578148 94-95 GTAAGCG 30 8.2862214E-5 39.578148 94-95 CGCAGAA 105 4.6267814E-8 36.18573 9 CTTTGCG 80 1.0736869E-5 35.62033 2 GGGTCCT 55 0.002575702 34.540928 7 GGTCCTA 55 0.002575702 34.540928 8 TAGGATA 85 1.6262857E-5 33.525017 4 TGGGGAG 60 0.0039449767 31.662518 6 TATAGGG 60 0.0039449767 31.662518 2 ATGCCGC 30 0.003950046 31.662518 94-95 TTGCGTT 100 4.935346E-5 28.496264 4 TTTGCGT 100 4.935346E-5 28.496264 3 TAGGGCG 35 0.008389693 27.1393 30-31 ACCCTTG 95 0.0012235945 24.996723 7 GGTCTAC 100 0.0016496724 23.746887 1 GGACAAT 140 1.7708093E-5 23.746887 6 GGTATCA 3405 0.0 23.572535 1 >>END_MODULE