##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938055_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69656 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.173452394625016 33.0 31.0 34.0 30.0 34.0 2 32.33363960032158 33.0 31.0 34.0 30.0 34.0 3 32.32990697140232 34.0 31.0 34.0 30.0 34.0 4 35.88450384747904 37.0 35.0 37.0 35.0 37.0 5 35.846904789250026 37.0 35.0 37.0 35.0 37.0 6 35.98511255311818 37.0 35.0 37.0 35.0 37.0 7 35.959845526587806 37.0 35.0 37.0 35.0 37.0 8 36.01847651315034 37.0 35.0 37.0 35.0 37.0 9 37.53032043183645 39.0 37.0 39.0 35.0 39.0 10-11 37.562614850120596 39.0 37.0 39.0 35.0 39.0 12-13 37.50953256000919 39.0 37.0 39.0 35.0 39.0 14-15 38.40500459400482 40.0 38.0 41.0 33.5 41.0 16-17 38.325930285976796 40.0 37.5 41.0 33.5 41.0 18-19 38.494257493970366 40.0 37.5 41.0 34.5 41.0 20-21 38.517923796944984 40.0 37.5 41.0 34.0 41.0 22-23 38.357944757092 40.0 37.0 41.0 34.0 41.0 24-25 38.13754737567474 40.0 36.5 41.0 34.0 41.0 26-27 37.78958740094177 40.0 35.5 41.0 33.5 41.0 28-29 37.51907947628345 39.0 35.0 41.0 33.0 41.0 30-31 37.02439129436085 38.5 35.0 41.0 33.0 41.0 32-33 36.327566900195244 38.0 35.0 40.0 32.5 41.0 34-35 35.76164293097507 37.5 35.0 40.0 31.0 41.0 36-37 35.42447168944527 37.5 35.0 40.0 30.0 41.0 38-39 34.936925749397034 37.0 35.0 40.0 28.5 41.0 40-41 34.7501363845182 37.0 35.0 40.0 27.5 41.0 42-43 34.195281095670154 36.5 35.0 40.0 24.0 41.0 44-45 33.63135264729528 36.0 34.0 40.0 20.5 41.0 46-47 32.84663919834615 36.0 33.0 40.0 5.5 41.0 48-49 32.103357930400826 36.0 33.0 40.0 2.0 41.0 50-51 31.301503100953255 35.0 32.5 40.0 2.0 41.0 52-53 30.711460606408636 35.0 31.0 39.0 2.0 41.0 54-55 30.23272223498335 35.0 30.5 39.0 2.0 41.0 56-57 29.489685023544276 35.0 28.5 39.0 2.0 41.0 58-59 28.74788245090157 35.0 26.0 39.0 2.0 40.0 60-61 27.81749598024578 35.0 22.5 38.0 2.0 40.0 62-63 27.08584328701045 35.0 19.0 38.0 2.0 40.0 64-65 26.33788905478351 34.0 5.0 37.5 2.0 39.5 66-67 25.399900941770987 33.5 2.0 36.5 2.0 39.0 68-69 24.602618582749514 33.0 2.0 36.0 2.0 39.0 70-71 23.853680946364996 33.0 2.0 35.0 2.0 38.0 72-73 23.226764384977606 32.0 2.0 35.0 2.0 37.0 74-75 22.729398759618697 31.0 2.0 35.0 2.0 37.0 76-77 21.676079591133572 29.5 2.0 33.5 2.0 35.0 78-79 22.168169289077753 31.0 2.0 35.0 2.0 36.0 80-81 21.910790168829678 31.0 2.0 35.0 2.0 35.0 82-83 21.512540197542208 30.5 2.0 35.0 2.0 35.0 84-85 21.025482370506488 30.0 2.0 34.5 2.0 35.0 86-87 20.622308200298612 29.5 2.0 34.0 2.0 35.0 88-89 20.042817560583437 29.0 2.0 34.0 2.0 35.0 90-91 19.595483519007693 27.5 2.0 34.0 2.0 35.0 92-93 18.96840186057195 25.5 2.0 34.0 2.0 35.0 94-95 18.341671930630525 25.0 2.0 34.0 2.0 35.0 96-97 17.609172217755827 21.5 2.0 33.5 2.0 35.0 98-99 16.703026300677614 9.5 2.0 33.0 2.0 35.0 100-101 13.98026731365568 2.0 2.0 29.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.06977607090906446 1101 2 0.1305783041835369 1101 3 0.2560943468392196 1101 4 -0.21683832368630362 1101 5 0.16168374899279314 1101 6 0.32244721216441263 1101 7 0.0753908250456945 1101 8 -0.23405992371109363 1101 9 0.19415001621251093 1101 10-11 -0.13104042369965185 1101 12-13 0.002162795633417147 1101 14-15 0.10231569577749866 1101 16-17 0.4080648278562009 1101 18-19 0.1690474282367873 1101 20-21 -6.226485119853464E-4 1101 22-23 -0.5699541168026983 1101 24-25 -0.2644316283843793 1101 26-27 -0.5674388573596545 1101 28-29 -0.5838937652539968 1101 30-31 -0.7790853285681436 1101 32-33 -1.5960232344915823 1101 34-35 -1.6430395685591677 1101 36-37 -1.5087281752382964 1101 38-39 -2.5981920795114846 1101 40-41 -1.8299259851397949 1101 42-43 -4.5436790991867895 1101 44-45 -3.861192364094123 1101 46-47 -3.8342120297867552 1101 48-49 -4.281667622450591 1101 50-51 -4.66622892755748 1101 52-53 -4.109544097016748 1101 54-55 -3.772780542683016 1101 56-57 -3.4045720796645718 1101 58-59 -3.559111650329225 1101 60-61 -3.199617819425576 1101 62-63 -3.3775434449660082 1101 64-65 -3.1100923978351567 1101 66-67 -2.644109543993803 1101 68-69 -3.2724143934573355 1101 70-71 -2.3874812142863533 1101 72-73 -1.76071932919902 1101 74-75 -2.4457730586114224 1101 76-77 -2.4092974261786964 1101 78-79 -2.4658053935943087 1101 80-81 -2.1616042889895226 1101 82-83 -2.5363000459693446 1101 84-85 -1.9292185121150247 1101 86-87 -2.27469601098322 1101 88-89 -1.6982271964811453 1101 90-91 -1.5812734730249502 1101 92-93 -2.3825171150128597 1101 94-95 -1.3011853284873212 1101 96-97 -1.117330119351891 1101 98-99 -0.8674518173180985 1101 100-101 0.599139221692436 1102 1 -0.07572314197656027 1102 2 0.07943349300304448 1102 3 0.16569886661323352 1102 4 0.013015973072540987 1102 5 -0.8903531228478272 1102 6 -0.8250401503095688 1102 7 -0.33495707861175816 1102 8 -0.5495520438419277 1102 9 0.45552378962910467 1102 10-11 0.19158818171218428 1102 12-13 0.31245122858018703 1102 14-15 0.5718369565565595 1102 16-17 0.4215944145347663 1102 18-19 1.9331431246882858E-6 1102 20-21 -0.045969065401671116 1102 22-23 -0.5485446609596991 1102 24-25 -0.3713302308226716 1102 26-27 -0.6190296988702002 1102 28-29 -0.8208845472938364 1102 30-31 -0.5013571097159328 1102 32-33 -0.9611733980360384 1102 34-35 -0.6753024291003555 1102 36-37 -1.1034352819882187 1102 38-39 -1.1639072148073453 1102 40-41 -0.4331076681609076 1102 42-43 -0.664850672187093 1102 44-45 -1.666574463410214 1102 46-47 -1.3322792316898173 1102 48-49 -0.664807675974231 1102 50-51 -0.46774543067969176 1102 52-53 -0.3417772222025981 1102 54-55 -0.339833768969072 1102 56-57 -0.5511673814784288 1102 58-59 -0.4722844127437362 1102 60-61 -0.4563826127648589 1102 62-63 -0.6390658951592876 1102 64-65 -0.3783053029793706 1102 66-67 0.20632161865859544 1102 68-69 0.03504918073237917 1102 70-71 -0.06024362879720613 1102 72-73 0.04139188102993785 1102 74-75 -0.2554667844514462 1102 76-77 0.18540968057123308 1102 78-79 -0.02943905401654945 1102 80-81 0.6116636265620699 1102 82-83 0.0337267158504595 1102 84-85 1.7132138399515817 1102 86-87 1.0628002720973626 1102 88-89 0.9025756581129656 1102 90-91 1.5401954535287246 1102 92-93 1.2614020048206243 1102 94-95 0.5324453583993893 1102 96-97 1.0041388072017803 1102 98-99 2.984317436324485 1102 100-101 2.745288493176231 1103 1 0.15133705673763842 1103 2 0.5355994778072528 1103 3 0.19287595603462648 1103 4 0.09834618932035255 1103 5 0.1955615469359273 1103 6 -0.3904989463352848 1103 7 -0.3919412983662838 1103 8 0.32099149794649406 1103 9 0.3184694354502611 1103 10-11 0.14512412560464583 1103 12-13 0.34532673997703256 1103 14-15 0.33250323358753775 1103 16-17 -0.2790192616779734 1103 18-19 0.23392946090466182 1103 20-21 -0.2348936708955378 1103 22-23 -0.041823445296337525 1103 24-25 -0.012767986521097896 1103 26-27 0.19511407669963887 1103 28-29 0.028326440432628885 1103 30-31 0.7156212655032377 1103 32-33 0.5544607340504584 1103 34-35 1.1293439765103983 1103 36-37 1.3525845894319986 1103 38-39 1.8511424637432086 1103 40-41 1.8244902278063648 1103 42-43 1.7352077731665005 1103 44-45 1.992104671598554 1103 46-47 2.9469434451073724 1103 48-49 3.070873212395142 1103 50-51 3.257999747578637 1103 52-53 4.048199399655967 1103 54-55 3.593976868085285 1103 56-57 4.081211344412864 1103 58-59 4.7695332377530555 1103 60-61 4.72582077706565 1103 62-63 5.192027032952929 1103 64-65 3.8914200038587374 1103 66-67 4.279816650803536 1103 68-69 4.763278286518467 1103 70-71 4.421199971436575 1103 72-73 4.686044131176054 1103 74-75 4.521710304969947 1103 76-77 3.677665671219973 1103 78-79 2.9932085205012733 1103 80-81 3.4601440473686154 1103 82-83 3.908799772542455 1103 84-85 3.919087597987815 1103 86-87 3.4486857192043168 1103 88-89 3.318560462321031 1103 90-91 3.915852963756663 1103 92-93 4.242407264902447 1103 94-95 4.989195796739541 1103 96-97 5.595313558809028 1103 98-99 6.716637716862181 1103 100-101 5.729508247705747 1104 1 0.7391603325997096 1104 2 0.38743137435897523 1104 3 0.4331776801725553 1104 4 0.23573843069966216 1104 5 -0.40465397030545347 1104 6 0.20594501918195363 1104 7 0.15762766715095466 1104 8 0.3182975324292556 1104 9 0.5765513320019835 1104 10-11 0.7068159359494715 1104 12-13 0.647859067563239 1104 14-15 1.2031928887599506 1104 16-17 0.775937634873749 1104 18-19 1.0493928229736298 1104 20-21 0.9350955532423981 1104 22-23 0.9756873305657265 1104 24-25 0.7773720996857989 1104 26-27 1.0746938180789485 1104 28-29 0.6670656645705648 1104 30-31 1.1404596275722056 1104 32-33 0.9211094409470135 1104 34-35 0.7723935454759143 1104 36-37 1.3067871756388953 1104 38-39 0.955398929260447 1104 40-41 0.6496518657373969 1104 42-43 0.7411344973044365 1104 44-45 1.3800357060813155 1104 46-47 0.7696805140728955 1104 48-49 -0.0999242013979611 1104 50-51 -0.3344032696627437 1104 52-53 -0.14792128999920706 1104 54-55 0.19446178187838825 1104 56-57 0.6614915168266577 1104 58-59 1.4007293584427067 1104 60-61 0.9523832770656497 1104 62-63 1.1262942743322384 1104 64-65 1.6026269004104599 1104 66-67 1.2014222542518134 1104 68-69 1.7118235451391577 1104 70-71 1.445715057643472 1104 72-73 1.7062488725553635 1104 74-75 1.6838870291078756 1104 76-77 1.439249722944112 1104 78-79 1.8288766239495509 1104 80-81 2.313861719782409 1104 82-83 2.177926927714868 1104 84-85 1.0928483738498862 1104 86-87 1.4314443398939716 1104 88-89 1.7598320140451698 1104 90-91 0.6739348603083855 1104 92-93 1.2927844200748595 1104 94-95 1.4706074346705762 1104 96-97 1.6847532139814412 1104 98-99 2.322241165138042 1104 100-101 1.3246052304643658 1105 1 0.19596180318794154 1105 2 0.2982769625942723 1105 3 0.4717806213490263 1105 4 0.09327519540554619 1105 5 0.25894897087101754 1105 6 0.08921707800548262 1105 7 0.32306884362154165 1105 8 0.34127547360572663 1105 9 0.4046763320019835 1105 10-11 0.01885637712594246 1105 12-13 0.3468480381514709 1105 14-15 0.15907524170112453 1105 16-17 0.25571704663845196 1105 18-19 0.49102885238539784 1105 20-21 0.5145992297129851 1105 22-23 0.43662483056572654 1105 24-25 0.810000776156393 1105 26-27 0.5209254357260065 1105 28-29 0.6234075763352749 1105 30-31 0.4947794805133796 1105 32-33 0.42616458800583956 1105 34-35 1.0816766337112043 1105 36-37 0.3812357050506634 1105 38-39 0.9999761351427949 1105 40-41 0.9423908363256288 1105 42-43 1.6097006737750235 1105 44-45 2.8506239413754315 1105 46-47 3.4654525728964245 1105 48-49 3.717171386837329 1105 50-51 4.299328347984314 1105 52-53 4.208696357059619 1105 54-55 4.991796340701917 1105 56-57 4.064984163885484 1105 58-59 3.7596447996191777 1105 60-61 3.8214090123597693 1105 62-63 4.503132509626354 1105 64-65 4.632038665116344 1105 66-67 5.208315636604755 1105 68-69 4.59187869219798 1105 70-71 4.913086381172885 1105 72-73 4.4236201960847765 1105 74-75 4.293262029107876 1105 76-77 3.788973987649996 1105 78-79 3.657001623949551 1105 80-81 4.392446278605938 1105 82-83 4.470665898303103 1105 84-85 3.618124109144002 1105 86-87 3.8432090457763266 1105 88-89 3.451927602280467 1105 90-91 3.318236330896621 1105 92-93 3.539567508310153 1105 94-95 3.798272875847047 1105 96-97 3.752767919863796 1105 98-99 3.855329400432158 1105 100-101 2.895836848111424 1106 1 0.354785332599711 1106 2 0.5932349457875432 1106 3 0.6465705373154123 1106 4 0.5352920021282301 1106 5 0.04088174398025757 1106 6 0.18585573346766893 1106 7 0.027270524293811604 1106 8 0.2589225324292528 1106 9 0.5475334748591294 1106 10-11 0.46507486452090063 1106 12-13 0.2199572818489557 1106 14-15 0.6317643173313812 1106 16-17 0.4857590634451796 1106 18-19 0.26077675154505897 1106 20-21 0.07594376752810916 1106 22-23 0.5759105448514461 1106 24-25 0.8392024568286516 1106 26-27 0.8749170323646567 1106 28-29 0.8177353074277036 1106 30-31 1.1496114132864932 1106 32-33 1.00347551237558 1106 34-35 0.8552060454759172 1106 36-37 0.954555032781748 1106 38-39 0.37749714354615804 1106 40-41 1.0262143657374025 1106 42-43 0.9817594973044379 1106 44-45 0.7728928489384614 1106 46-47 -0.12005162878424613 1106 48-49 0.15855794145918622 1106 50-51 0.12474851605153958 1106 52-53 0.8538644242865097 1106 54-55 0.49200642473552847 1106 56-57 0.8780093739695154 1106 58-59 -0.15485099870015162 1106 60-61 0.09724934849421984 1106 62-63 0.07182998861795298 1106 64-65 0.034769757553316794 1106 66-67 -0.19210453146247275 1106 68-69 0.41204675942487157 1106 70-71 0.2374561290720436 1106 72-73 1.0587042296982219 1106 74-75 0.7825477433935895 1106 76-77 1.2202765086583973 1106 78-79 1.4641444810924078 1106 80-81 1.092656362639552 1106 82-83 1.4937751420005831 1106 84-85 1.4511073024213168 1106 86-87 1.1171586256082549 1106 88-89 1.104028442616599 1106 90-91 2.03777414602267 1106 92-93 2.734525491503433 1106 94-95 3.4266342203848623 1106 96-97 3.6231460711242995 1106 98-99 3.8025983079951864 1106 100-101 3.5962570161786527 1107 1 0.46690654472092064 1107 2 0.7127154652680687 1107 3 0.2712458619907352 1107 4 0.4578028246390531 1107 5 0.3084142115127264 1107 6 0.5170245646365004 1107 7 0.4398246368479235 1107 8 0.18619525970198225 1107 9 0.11679754412319454 1107 10-11 0.5077155571615961 1107 12-13 0.655671567563239 1107 14-15 0.8774353130023727 1107 16-17 0.5117330894192023 1107 18-19 0.924629565397872 1107 20-21 0.813173204757554 1107 22-23 0.8873824063233045 1107 24-25 0.6426656602918612 1107 26-27 1.4333585908062219 1107 28-29 0.9703327100251116 1107 30-31 1.1790486427237212 1107 32-33 1.647198456098529 1107 34-35 1.9658121060819767 1107 36-37 1.8716545998813174 1107 38-39 1.6980599141089314 1107 40-41 1.9140931536161858 1107 42-43 1.8817594973044365 1107 44-45 2.25124782729344 1107 46-47 2.700078241345622 1107 48-49 1.7793371622383987 1107 50-51 1.8178221091251316 1107 52-53 2.463821134243215 1107 54-55 2.3476341303632395 1107 56-57 3.005715001675142 1107 58-59 2.896231252382101 1107 60-61 3.8091541103989854 1107 62-63 4.119665486453449 1107 64-65 4.250354173137733 1107 66-67 4.2239127845548445 1107 68-69 4.078064075442189 1107 70-71 3.735724527340441 1107 72-73 3.62883409982809 1107 74-75 3.5394741503199967 1107 76-77 3.286077374459264 1107 78-79 3.831244048191973 1107 80-81 4.187678007661198 1107 82-83 3.8552470034724458 1107 84-85 3.9316267829407963 1107 86-87 3.6435655520151826 1107 88-89 4.0330327716209275 1107 90-91 5.05509016333869 1107 92-93 4.893399950377891 1107 94-95 5.033344177094818 1107 96-97 5.314488062466289 1107 98-99 5.070347225744104 1107 100-101 5.32720939713103 1108 1 0.46066768554088355 1108 2 0.4600416684766202 1108 3 0.5158982684078524 1108 4 0.2697457836408361 1108 5 -0.07928632324662743 1108 6 0.26568766624077966 1108 7 0.16130413773918661 1108 8 0.20892253242925563 1108 9 -0.38944131505684254 1108 10-11 0.3276799065377034 1108 12-13 0.5527303910926449 1108 14-15 1.1370164181717186 1108 16-17 0.895422928991394 1108 18-19 0.4616170876795138 1108 20-21 0.30136393559534014 1108 22-23 0.5175071835069005 1108 24-25 0.39088312909756695 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0.7973943422709233 2316 66-67 1.37384231239135 2316 68-69 1.5361331381624126 2316 70-71 1.853236859969055 2316 72-73 2.703886953950711 2316 74-75 2.4822155174799683 2316 76-77 2.0822366415487643 2316 78-79 2.0058388332518753 2316 80-81 2.161427126759154 2316 82-83 2.558559195156729 2316 84-85 2.6620707575708167 2316 86-87 2.9198164329172265 2316 88-89 3.7754570140451698 2316 90-91 3.6357807905409416 2316 92-93 3.75263180379579 2316 94-95 3.337431562577553 2316 96-97 3.332282283748885 2316 98-99 3.1260202349054858 2316 100-101 3.2826357537201805 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 3.0 5 12.0 6 29.0 7 52.0 8 81.0 9 127.0 10 253.0 11 426.0 12 611.0 13 858.0 14 998.0 15 1358.0 16 1725.0 17 2120.0 18 2060.0 19 1670.0 20 1636.0 21 1699.0 22 1766.0 23 1857.0 24 1890.0 25 1870.0 26 1464.0 27 1162.0 28 1346.0 29 1624.0 30 1794.0 31 2043.0 32 2507.0 33 3240.0 34 4990.0 35 8351.0 36 6893.0 37 7739.0 38 3252.0 39 148.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.46835879177673 23.40932582979212 15.361203629263812 14.761111749167336 2 14.848158518199439 27.890013640605932 36.094479144231464 21.16734869696317 3 23.444454015562638 31.9909840066615 33.09357682258018 11.470985155195681 4 10.471459745032732 25.28568967497416 34.05018950269898 30.19266107729413 5 7.562976907269828 44.23112848294989 33.40757878400321 14.798315825777062 6 20.657229815091306 40.563626966808314 14.624727230963591 24.154415987136787 7 15.830653497186173 43.38893993338693 20.49643964626163 20.28396692316527 8 22.842253359366026 31.414379235098195 20.292580682209717 25.450786723326058 9 19.927931549328125 23.04611232341794 22.83220397381417 34.19375215343976 10-11 22.128746985184332 30.171126679683013 23.59595727575514 24.10416905937751 12-13 28.395256690019526 29.322671413804986 24.835620764901805 17.446451131273687 14-15 22.695101642356725 30.97723096359251 29.461927185023544 16.86574020902722 16-17 15.359768002756402 35.43700470885495 29.372200528310554 19.8310267600781 18-19 14.674256345469164 33.01438497760423 34.21528655105088 18.096072125875732 20-21 17.515361203629265 29.18485126909383 33.17302170667279 20.126765820604113 22-23 15.027636207020315 32.12188644031297 34.98887373483598 17.86160361783074 24-25 18.627971746870333 33.14430917652464 33.79823705064891 14.429482025956128 26-27 17.11629576453697 30.889447236180906 38.21464465183058 13.779612347451545 28-29 13.787756977144827 34.83763064201217 36.72619731250718 14.64841506833582 30-31 15.506919719765705 34.050907315952685 34.46149649707132 15.980676467210293 32-33 17.268433444355118 32.456644079476284 36.39887446881819 13.876048007350409 34-35 16.553719364240692 31.124639262588122 35.890680411779066 16.430960961392124 36-37 13.886097392902263 32.79329849546342 38.38649936832434 14.93410474330998 38-39 14.650481673438328 30.91539488607813 38.19074555295536 16.243377887528176 40-41 13.785256387247758 31.851171204801116 37.862614051586135 16.500958356364993 42-43 14.58391656078845 31.818018677634928 35.95408833473308 17.643976426843537 44-45 14.452970776773602 30.4134034901333 36.80217074518868 18.331454987904415 46-47 17.32944757091995 30.515677041460894 35.87917767313656 16.2756977144826 48-49 15.805530033306535 29.00826920868267 36.6027334328701 18.58346732514069 50-51 12.121712415298036 30.092885035029287 39.72163202021362 18.063770529459056 52-53 14.37492821867463 31.348340415757438 36.6565694268979 17.620161938670034 54-55 13.135264729527965 29.004680142414152 36.034225335936604 21.825829792121283 56-57 16.872200528310554 29.58467325140691 33.102676007809805 20.440450212472726 58-59 17.180142414149536 30.771936373033192 34.404071436775006 17.643849776042263 60-61 15.313110141265648 34.187291834156426 33.26059492362467 17.23900310095326 62-63 12.8847479039853 35.29200643160675 32.63753301940967 19.185712644998276 64-65 12.735442747214885 37.156167451475824 30.985126909383254 19.12326289192604 66-67 14.49193177902837 33.99850694843229 29.657890203284715 21.85167106925462 68-69 13.57743769380958 33.167997013896866 31.322499138624092 21.93206615366946 70-71 16.600867118410477 32.29082921787068 29.96152520960147 21.146778454117378 72-73 15.551424141495348 29.76125531181808 33.32232686344321 21.364993683243366 74-75 14.67569197197657 32.61528080854485 30.338377167796025 22.370650051682553 76-77 15.27865510508786 29.389428046399445 31.683559205237167 23.648357643275524 78-79 15.624641093373148 29.740438727460667 32.60738486275411 22.027535316412084 80-81 17.05093602848283 29.024778913517856 31.254306879522225 22.669978178477088 82-83 15.47975393185032 28.913725405746852 33.15603218697734 22.450488475425487 84-85 15.85037578333058 29.836550402342993 31.517705245174398 22.795368569152032 86-87 15.792004938590635 30.45847061610354 30.472826984229528 23.276697461076296 88-89 15.496870334213849 30.149592282071897 28.81015275066039 25.54338463305387 90-91 16.61665900999196 28.22011025611577 30.153899161594122 25.009331572298148 92-93 17.544791547031124 29.433932468129093 30.11872631216263 22.902549672677157 94-95 16.587946479843804 28.3356781899621 30.30248650511083 24.773888825083265 96-97 14.420150453657977 29.504278166992076 31.540714367750084 24.53485701159986 98-99 16.375473756747443 29.54016882967727 28.789336166302977 25.29502124727231 100-101 16.05963592511772 28.15981394280464 29.73397840817733 26.046571723900307 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3052.0 1 1733.5 2 465.0 3 618.0 4 483.0 5 290.0 6 523.5 7 896.5 8 1134.5 9 1113.5 10 783.5 11 443.0 12 326.5 13 309.5 14 298.5 15 272.5 16 271.5 17 266.0 18 280.0 19 301.5 20 356.0 21 423.0 22 442.5 23 502.5 24 585.5 25 641.0 26 743.5 27 841.0 28 912.0 29 1015.5 30 1092.5 31 1153.0 32 1213.5 33 1236.5 34 1226.5 35 1186.0 36 1122.0 37 1066.5 38 1021.0 39 984.0 40 1014.5 41 1105.5 42 1220.0 43 1288.0 44 1343.0 45 1416.5 46 1711.5 47 3227.0 48 5954.0 49 6299.5 50 4632.5 51 3401.5 52 1989.5 53 1034.5 54 693.5 55 570.0 56 504.0 57 426.5 58 354.5 59 289.5 60 233.5 61 193.0 62 152.5 63 120.0 64 93.0 65 67.5 66 57.5 67 44.0 68 28.5 69 21.0 70 13.0 71 12.0 72 12.0 73 9.0 74 6.5 75 4.0 76 4.5 77 4.5 78 2.5 79 1.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01579189158148616 3 0.0028712530148156654 4 0.0 5 0.0961869759963248 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014356265074078327 24-25 0.0 26-27 0.008613759044446997 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01004938555185483 36-37 0.0 38-39 0.004306879522223498 40-41 0.00789594579074308 42-43 7.178132537039164E-4 44-45 0.0035890662685195817 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.178132537039164E-4 84-85 0.002153439761111749 86-87 7.178132537039164E-4 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 69656.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 41.959055932008724 #Duplication Level Percentage of deduplicated Percentage of total 1 87.59366339343757 36.75347421614792 2 6.6616484757245 5.590329619846101 3 2.172648578369316 2.7348684966119214 4 1.115406986690389 1.872056965659814 5 0.5405960242241763 1.134144940852188 6 0.3045129503541246 0.7666245549557826 7 0.2155541109248298 0.6331112897668543 8 0.15738871591336778 0.5283105547260825 9 0.14370274061655317 0.5426668198001608 >10 0.8280015054572827 6.506259331572298 >50 0.10264481472610942 3.0923394969564715 >100 0.10948780237451672 11.489318938784885 >500 0.023950456769425532 6.196164005972206 >1k 0.030793444417832827 22.160330768347304 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3294 4.728953715401401 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2031 2.9157574365453085 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1845 2.6487309061674513 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1811 2.599919604915585 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 1770 2.5410589181118644 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1282 1.8404731824968417 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1236 1.7744343631560813 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1126 1.6165154473412195 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 1041 1.4944871942115538 RNA PCR Primer, Index 46 (95% over 21bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 749 1.0752842540484668 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 633 0.908751579189158 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 616 0.884345928563225 RNA PCR Primer, Index 46 (95% over 23bp) ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 608 0.8728609165039622 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 595 0.8541977719076604 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 581 0.8340990008039508 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 534 0.7666245549557826 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 484 0.6948432295853911 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 454 0.6517744343631561 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 450 0.6460319283335247 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 448 0.6431606753187091 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 442 0.6345469162742621 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 436 0.625933157229815 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 409 0.5871712415298036 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 382 0.5484093258297921 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 343 0.49241989204088665 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 312 0.4479154703112438 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 304 0.4364304582519811 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 268 0.3847479039852992 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 267 0.3833122774778913 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 247 0.3545997473297347 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 237 0.34024348225565637 RNA PCR Primer, Index 46 (96% over 26bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 211 0.3029171930630527 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 200 0.28712530148156656 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 195 0.27994716894452737 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 187 0.2684621568852647 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 178 0.25554151831859423 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 177 0.2541058918111864 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 169 0.24262087975192373 TruSeq Adapter, Index 4 (95% over 23bp) ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 152 0.21821522912599056 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 142 0.20385896405191226 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 134 0.1923739519926496 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 121 0.17371080739634778 No Hit TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT 120 0.17227518088893992 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 116 0.1665326748593086 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 107 0.1536120362926381 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 107 0.1536120362926381 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 103 0.14786953026300678 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 101 0.14499827724819112 No Hit CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 98 0.14069139772596762 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96 0.13782014471115195 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 94 0.13494889169633628 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 88 0.12633513265188928 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 87 0.12489950614448145 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 87 0.12489950614448145 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 86 0.12346387963707361 No Hit CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 83 0.11915700011485013 No Hit ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC 81 0.11628574710003446 TruSeq Adapter, Index 18 (95% over 24bp) AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 79 0.1134144940852188 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 79 0.1134144940852188 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 76 0.10910761456299528 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 75 0.10767198805558746 No Hit GTACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 73 0.1048007350407718 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 72 0.10336510853336398 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.10336510853336398 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 70 0.10049385551854831 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 70 0.10049385551854831 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0028712530148156654 0.0 22-23 0.0 0.0 0.0 0.020816584357413577 0.0 24-25 0.0 0.0 0.0 0.04737567474445848 0.0 26-27 0.0 0.0 0.0 0.09403353623521304 0.0 28-29 0.0 0.0 0.0 0.24262087975192376 0.0 30-31 0.0 0.0 0.0 0.5304639944871942 0.0 32-33 0.0 0.0 0.0 0.969765705753991 0.0 34-35 0.0 0.0 0.0 1.6136441943264042 0.0 36-37 0.0 0.0 0.0 2.3824221890432984 0.0 38-39 0.0 0.0 0.0 3.2136499368324336 0.0 40-41 0.0 0.0 0.0 3.9975020098771106 0.0 42-43 0.0 0.0 0.0 4.659325829792122 0.0 44-45 0.0 0.0 0.0 5.33909498104973 0.0 46-47 0.0 0.0 0.0 5.999483174457334 0.0 48-49 0.0 0.0 0.0 6.601728494314919 0.0 50-51 0.0 0.0 0.0 7.147984380383599 0.0 52-53 0.0 0.0 0.0 7.612409555530033 0.0 54-55 0.0 0.0 0.0 8.167997013896866 0.0 56-57 0.0 0.0 0.0 8.875043068795222 0.0 58-59 0.0 0.0 0.0 9.339468243941656 0.0 60-61 0.0 0.0 0.0 9.789537154014013 0.0 62-63 0.0 0.0 0.0 10.197255082117836 0.0 64-65 0.0 0.0 0.0 10.59564143792351 0.0 66-67 0.0 0.0 0.0 11.051452854025497 0.0 68-69 0.0 0.0 0.0 11.597708740094177 0.0 70-71 0.0 0.0 0.0 12.106638336970255 0.0 72-73 0.0 0.0 0.0 12.557425060296314 0.0 74-75 0.0 0.0 0.0 13.062047777650168 0.0 76-77 0.0 0.0 0.0 13.490582290111405 0.0 78-79 0.0 0.0 0.0 14.126564832893074 0.0 80-81 0.0 0.0 0.0 14.718760767198805 0.0 82-83 0.0 0.0 0.0 15.178879062823016 0.0 84-85 0.0 0.0 0.0 15.649764557252785 0.0 86-87 0.0 0.0 0.0 16.13859538302515 0.0 88-89 0.0 0.0 0.0 16.707103479958654 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTACAC 20 0.0020604306 71.29742 3 TGGGCAG 30 1.2930359E-4 63.329987 6 CGCAGAA 115 1.8189894E-12 45.432377 9 ATGGGCA 50 0.0016001671 38.025288 5 TGGGTAC 30 0.003940166 31.664993 48-49 AAGAATG 30 0.003940166 31.664993 76-77 AGTACTC 120 5.2303694E-6 27.746706 2 CCCACTT 35 0.00836888 27.141422 86-87 AGTAAAA 70 1.7656841E-5 23.748745 12-13 CTTGAAA 600 0.0 22.561306 56-57 TGAAAAA 605 0.0 22.374851 58-59 ATGGGGA 65 2.9246302E-4 21.921917 18-19 GCAGAAA 115 8.8833986E-8 20.651081 10-11 CATGGGG 140 4.7676085E-4 20.370691 4 CATGGGC 190 8.02371E-6 20.01331 4 TGCTTGA 680 0.0 19.907036 54-55 GAGTAAA 60 0.004744347 19.79062 10-11 AGTACTT 1095 0.0 19.085749 12-13 GGCTTGA 100 2.7396536E-4 18.998995 8 TACATGG 690 0.0 18.612696 2 >>END_MODULE