##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938054_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 69382 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18362111210401 33.0 31.0 34.0 30.0 34.0 2 32.376956559338154 34.0 31.0 34.0 31.0 34.0 3 32.25310599290882 34.0 31.0 34.0 30.0 34.0 4 35.85432821192816 37.0 35.0 37.0 35.0 37.0 5 35.86536853939062 37.0 35.0 37.0 35.0 37.0 6 36.00994494249229 37.0 35.0 37.0 35.0 37.0 7 35.96523594015739 37.0 35.0 37.0 35.0 37.0 8 36.03817993139431 37.0 35.0 37.0 35.0 37.0 9 37.570955002738465 39.0 37.0 39.0 35.0 39.0 10-11 37.58963419907181 39.0 37.0 39.0 35.0 39.0 12-13 37.54562422530339 39.0 37.0 39.0 35.0 39.0 14-15 38.46863739874895 40.0 38.0 41.0 34.0 41.0 16-17 38.3899354299386 40.0 38.0 41.0 33.5 41.0 18-19 38.412549364388454 40.0 37.0 41.0 34.0 41.0 20-21 38.48746072468363 40.0 37.5 41.0 34.0 41.0 22-23 38.387240206393585 40.0 37.0 41.0 34.0 41.0 24-25 38.2087717275374 40.0 36.5 41.0 34.0 41.0 26-27 37.817877835749904 40.0 35.5 41.0 33.5 41.0 28-29 37.600869101496066 39.5 35.0 41.0 33.0 41.0 30-31 37.125616154045716 39.0 35.0 41.0 33.0 41.0 32-33 36.43448588971203 38.0 35.0 40.5 32.5 41.0 34-35 35.937354068778646 38.0 35.0 40.0 31.0 41.0 36-37 35.50680291718313 37.0 35.0 40.0 30.5 41.0 38-39 35.06861289671673 37.0 35.0 40.0 29.5 41.0 40-41 34.88899138104984 37.0 35.0 40.0 28.0 41.0 42-43 34.40778588106426 37.0 35.0 40.0 25.0 41.0 44-45 33.85766481219913 37.0 35.0 40.0 21.0 41.0 46-47 33.050762445591076 36.0 33.5 40.0 5.5 41.0 48-49 32.319902856648696 36.0 33.0 40.0 2.0 41.0 50-51 31.621191375284656 35.0 33.0 40.0 2.0 41.0 52-53 31.091154766365918 35.0 32.0 40.0 2.0 41.0 54-55 30.650492923236573 35.0 31.0 40.0 2.0 41.0 56-57 29.999754979677725 35.0 30.0 39.5 2.0 41.0 58-59 29.36011501542187 35.0 27.5 39.0 2.0 41.0 60-61 28.478971491164856 35.0 26.0 39.0 2.0 40.5 62-63 27.86725663716814 35.0 22.5 38.0 2.0 40.0 64-65 27.12085267072151 35.0 13.5 38.0 2.0 40.0 66-67 26.137838344239142 34.5 2.0 37.0 2.0 39.5 68-69 25.239860482545907 34.0 2.0 36.5 2.0 39.0 70-71 24.407274220979502 33.0 2.0 35.5 2.0 38.5 72-73 23.91208814966418 33.0 2.0 35.0 2.0 37.0 74-75 23.47794817099536 33.0 2.0 35.0 2.0 37.0 76-77 22.446333342941973 30.5 2.0 34.0 2.0 35.5 78-79 22.944531722925255 32.0 2.0 35.0 2.0 36.0 80-81 22.823506096682138 32.0 2.0 35.0 2.0 36.0 82-83 22.50803522527457 32.0 2.0 35.0 2.0 35.0 84-85 22.119887002392552 31.0 2.0 35.0 2.0 35.0 86-87 21.821466662823212 31.0 2.0 35.0 2.0 35.0 88-89 21.347323513303163 30.5 2.0 35.0 2.0 35.0 90-91 20.977126632267733 30.0 2.0 34.0 2.0 35.0 92-93 20.55469718370759 29.0 2.0 34.0 2.0 35.0 94-95 20.021396039318553 29.0 2.0 34.0 2.0 35.0 96-97 19.445353261652876 28.0 2.0 34.0 2.0 35.0 98-99 18.855229021936523 27.0 2.0 34.0 2.0 35.0 100-101 16.597323513303163 13.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.00996136702840289 1101 2 0.07302229803170235 1101 3 0.11019604370279268 1101 4 0.07119620352752065 1101 5 0.2552145531853327 1101 6 0.3170832719529031 1101 7 0.132632569129278 1101 8 0.4116122959312847 1101 9 0.6629307193336729 1101 10-11 0.39121326301461323 1101 12-13 0.5723394341887129 1101 14-15 0.6619002749842622 1101 16-17 0.14585955148172758 1101 18-19 0.9545615884785477 1101 20-21 0.0016379170907114826 1101 22-23 0.42747548026053295 1101 24-25 0.3398319688460063 1101 26-27 0.8361688297232419 1101 28-29 0.4807301027120232 1101 30-31 0.39747499370967887 1101 32-33 0.9801035968655967 1101 34-35 1.6270875907509108 1101 36-37 1.4551509566960732 1101 38-39 1.172798377491084 1101 40-41 0.7634020364749006 1101 42-43 0.9866825244658131 1101 44-45 0.7879911739134613 1101 46-47 0.8056322593216052 1101 48-49 1.1421318050179465 1101 50-51 1.5569429475625718 1101 52-53 1.5762859045046334 1101 54-55 2.2754058005302973 1101 56-57 2.1444666679915194 1101 58-59 2.724340660703504 1101 60-61 3.0006335186210364 1101 62-63 3.303651767594083 1101 64-65 3.181764307734266 1101 66-67 3.5175882364897504 1101 68-69 3.803487335102261 1101 70-71 4.251768998815063 1101 72-73 4.093699576561292 1101 74-75 4.003519628762962 1101 76-77 4.037158436694533 1101 78-79 4.166128299125205 1101 80-81 3.8852786204396494 1101 82-83 4.5165358898881465 1101 84-85 3.6922061886277326 1101 86-87 3.879244179241514 1101 88-89 4.089811512887788 1101 90-91 3.222214167183349 1101 92-93 3.5977681703975186 1101 94-95 3.190899920210832 1101 96-97 2.4668114281194917 1101 98-99 3.9982418821720245 1101 100-101 3.8863571737794445 1102 1 -0.04385967211315034 1102 2 -0.4354522782394845 1102 3 -0.29658361731415894 1102 4 -0.19999023715045183 1102 5 -0.7786837518994147 1102 6 -0.6659675755047232 1102 7 -0.5622826851080092 1102 8 -1.0460148227127846 1102 9 -0.9472387721917528 1102 10-11 -0.6342104657989509 1102 12-13 -0.1479995488621384 1102 14-15 0.05173078345884363 1102 16-17 -0.10837773665385697 1102 18-19 -0.7234045132163729 1102 20-21 -0.09158242189234045 1102 22-23 -0.11489740109539781 1102 24-25 -0.40593074301839494 1102 26-27 -0.7740006618021766 1102 28-29 -0.48537159220322934 1102 30-31 -0.17032161645981603 1102 32-33 -0.748709962456438 1102 34-35 -0.3729124092490892 1102 36-37 0.005998414323194368 1102 38-39 -0.20008297844112377 1102 40-41 0.25492746020371726 1102 42-43 0.7748181176861451 1102 44-45 0.16934710611685233 1102 46-47 0.1615644627114321 1102 48-49 0.7777250253569292 1102 50-51 0.2942310831557897 1102 52-53 -0.3050700276987577 1102 54-55 -0.7330687757408931 1102 56-57 -0.5928214676016985 1102 58-59 -0.2756593392964959 1102 60-61 0.2463962304854448 1102 62-63 -0.2895685713889691 1102 64-65 0.1987134602766396 1102 66-67 1.1447068805575462 1102 68-69 1.2272161486615865 1102 70-71 1.9043113716964193 1102 72-73 1.822513135883323 1102 74-75 1.2238586118138066 1102 76-77 0.7066499621182594 1102 78-79 1.3101960957353747 1102 80-81 1.3513803153549055 1102 82-83 0.9656884322610289 1102 84-85 1.3023756801531547 1102 86-87 1.8538204504279534 1102 88-89 0.8525233772945633 1102 90-91 1.4001802688782625 1102 92-93 2.0214969839568404 1102 94-95 1.9536117846176104 1102 96-97 0.9413876993059347 1102 98-99 1.6846825601381248 1102 100-101 0.9456792076777489 1103 1 0.24359795500549097 1103 2 -0.06460482061236661 1103 3 0.5342638403129598 1103 4 -0.024058033760617548 1103 5 -0.11732781969602257 1103 6 0.1381002211054394 1103 7 0.42788680641741905 1103 8 0.3278834823719663 1103 9 -0.41130656880191907 1103 10-11 0.21358614437054513 1103 12-13 0.1721699426632881 1103 14-15 0.7139341732893527 1103 16-17 0.8111137887698661 1103 18-19 0.7532056562751563 1103 20-21 0.24163791709070637 1103 22-23 0.4354415819554518 1103 24-25 0.6289845112188956 1103 26-27 -0.11094981434455065 1103 28-29 0.02445891627134955 1103 30-31 0.39289872252323477 1103 32-33 0.6672222409333912 1103 34-35 0.8177655568526063 1103 36-37 0.8844729905943822 1103 38-39 0.6124593944402292 1103 40-41 -0.09914033640644959 1103 42-43 0.3276994736183525 1103 44-45 -1.068280012527218 1103 46-47 -1.2379270627122878 1103 48-49 -0.7524444661684981 1103 50-51 -0.3471248490475993 1103 52-53 -0.34015477346147094 1103 54-55 0.5301515632421605 1103 56-57 0.8588734476525381 1103 58-59 1.075696592906894 1103 60-61 1.8952097898074811 1103 62-63 2.107889055729675 1103 64-65 1.7449846467173167 1103 66-67 0.534876372082973 1103 68-69 0.5694195384920917 1103 70-71 1.3536334055947243 1103 72-73 1.2696317799511228 1103 74-75 1.296570476220591 1103 76-77 0.7463109790674096 1103 78-79 0.28273846861673135 1103 80-81 -0.5096366337976406 1103 82-83 0.09416300853221315 1103 84-85 0.589324832695528 1103 86-87 1.0311085860211726 1103 88-89 0.9959132078030386 1103 90-91 1.567298912946061 1103 92-93 1.253530882261927 1103 94-95 0.558188055804056 1103 96-97 1.579353801000849 1103 98-99 1.9424791703076174 1103 100-101 2.24364530937266 1104 1 0.40359795500549467 1104 2 0.015395179387631686 1104 3 0.47734076338988274 1104 4 0.2020958123932317 1104 5 0.3180567956885909 1104 6 0.17810022110544566 1104 7 -0.182882424351817 1104 8 0.08019117467965486 1104 9 0.1933088158134666 1104 10-11 0.2028169136013176 1104 12-13 0.20832378881713254 1104 14-15 0.3993187886739591 1104 16-17 0.30726763492371134 1104 18-19 0.5578210408905377 1104 20-21 0.547791763244561 1104 22-23 0.20544158195545492 1104 24-25 0.6428306650650413 1104 26-27 0.7244348010400685 1104 28-29 0.8506127624251931 1104 30-31 0.6844371840617001 1104 32-33 0.7687607024718446 1104 34-35 0.6754578645449101 1104 36-37 1.4913960675174494 1104 38-39 1.7447670867479204 1104 40-41 1.772398125132007 1104 42-43 1.3092379351568155 1104 44-45 1.8886430643958576 1104 46-47 2.4074575526723194 1104 48-49 2.4675555338315007 1104 50-51 2.4513366894139317 1104 52-53 3.1129221496154464 1104 54-55 1.898613101703699 1104 56-57 1.8934888322679235 1104 58-59 1.6441581313684352 1104 60-61 2.298286712884405 1104 62-63 2.296350594191214 1104 64-65 3.059600031332703 1104 66-67 2.6164148336214303 1104 68-69 2.4594195384920923 1104 70-71 2.4559410979024143 1104 72-73 2.418862549181892 1104 74-75 2.6327243223744325 1104 76-77 2.1247725175289496 1104 78-79 1.7550461609244223 1104 80-81 2.3980556738946674 1104 82-83 2.243393777762982 1104 84-85 2.3654786788493745 1104 86-87 2.4911085860211735 1104 88-89 3.278990130879965 1104 90-91 2.9657604514076006 1104 92-93 3.735838574569616 1104 94-95 2.9897265173425147 1104 96-97 3.4039691856162335 1104 98-99 4.66786378569223 1104 100-101 3.7544145401418945 1105 1 0.48052103192856777 1105 2 0.5538567178491718 1105 3 0.4158023018514214 1105 4 0.47901888931630765 1105 5 0.1795952572270565 1105 6 0.03963868264390413 1105 7 0.17096372949433913 1105 8 0.03403732852581243 1105 9 0.47023189273654253 1105 10-11 -0.34333693255253195 1105 12-13 0.07755455804790046 1105 14-15 0.4223957117508874 1105 16-17 0.49957532723140474 1105 18-19 0.3578210408905349 1105 20-21 0.8093302247830181 1105 22-23 0.743903120416995 1105 24-25 0.9197537419881243 1105 26-27 0.901357877963143 1105 28-29 0.5813819931944195 1105 30-31 0.907514107138617 1105 32-33 1.407222240933386 1105 34-35 1.5831501722372252 1105 36-37 1.4606268367482258 1105 38-39 0.8063055482863817 1105 40-41 0.43393658667046964 1105 42-43 -0.06768514176626184 1105 44-45 0.1578738336266312 1105 46-47 0.10745755267232227 1105 48-49 -0.824752158476187 1105 50-51 -0.571740233662986 1105 52-53 -0.5563086196153151 1105 54-55 -0.26292535983476384 1105 56-57 0.23964267842177023 1105 58-59 -0.248149560939261 1105 60-61 0.1675174821151728 1105 62-63 0.16558136342198182 1105 64-65 0.6749846467173164 1105 66-67 0.6933379105445105 1105 68-69 1.0901887692613208 1105 70-71 1.2790180209793398 1105 72-73 1.6957856261049677 1105 74-75 1.2634935531436646 1105 76-77 1.7093879021443357 1105 78-79 1.5858153916936537 1105 80-81 1.259594135433133 1105 82-83 1.443393777762985 1105 84-85 1.4808632942339912 1105 86-87 2.052647047559635 1105 88-89 2.4328362847261182 1105 90-91 2.227298912946061 1105 92-93 1.7743001130311562 1105 94-95 1.7204957481117482 1105 96-97 1.8808922625393087 1105 98-99 1.483248401076846 1105 100-101 0.8928760786034324 1106 1 -0.4186242672167282 1106 2 -0.06714450315205056 1106 3 0.13521622126533828 1106 4 0.013084823382236266 1106 5 -0.07193099429920125 1106 6 -0.037772794767576556 1106 7 0.032013790544397125 1106 8 -0.42481493032644835 1106 9 -0.03575101324636165 1106 10-11 -0.5981598873754876 1106 12-13 -0.3156078351144913 1106 14-15 -1.0954309060757268 1106 16-17 -0.6531719255158492 1106 18-19 -0.8633022802327801 1106 20-21 -0.061854146401351784 1106 22-23 -0.053288576774704666 1106 24-25 -0.37768215544777206 1106 26-27 -0.9099974333921708 1106 28-29 -0.047922036109603994 1106 30-31 -0.37868857906406816 1106 32-33 -0.186428552717409 1106 34-35 -0.6306471415600896 1106 36-37 -0.32679685067546416 1106 38-39 -0.9443660023851663 1106 40-41 -1.349457796723911 1106 42-43 -1.5189671930483186 1106 44-45 -1.835899060146268 1106 46-47 -2.2222127769980062 1106 48-49 -2.062603196327224 1106 50-51 -1.9045851665079176 1106 52-53 -1.4355515988582965 1106 54-55 -0.9493722462816514 1106 56-57 -0.4038249650458745 1106 58-59 0.0580775452878477 1106 60-61 -1.2232029086052165 1106 62-63 -0.4817934839528668 1106 64-65 -0.4869201151874414 1106 66-67 -0.16575854855194905 1106 68-69 0.14354652261907574 1106 70-71 0.1144270563883758 1106 72-73 0.44169527201461634 1106 74-75 0.3119673016174147 1106 76-77 0.3751998679563009 1106 78-79 0.5659130717913321 1106 80-81 1.1665538423928368 1106 82-83 0.4503534847226902 1106 84-85 0.7796422930129907 1106 86-87 -0.15492316001057205 1106 88-89 -0.35678520489537036 1106 90-91 -0.6393677537206024 1106 92-93 -0.46599292726188324 1106 94-95 -0.6194309918149941 1106 96-97 -1.4282652466181993 1106 98-99 -0.6126001947717512 1106 100-101 0.15539134111869402 1107 1 0.03216938357692101 1107 2 -0.0988905348980822 1107 3 0.1399781260272448 1107 4 -0.18691517661775947 1107 5 -0.14018496255316393 1107 6 -0.4504712074659878 1107 7 -0.09497033643972941 1107 8 -0.15497366048518302 1107 9 0.09440771691236449 1107 10-11 -0.03784242705803109 1107 12-13 0.02645565694900398 1107 14-15 0.026791316146493216 1107 16-17 0.23968521734129666 1107 18-19 -0.355365772296274 1107 20-21 -0.17693351148071912 1107 22-23 -0.23027270375883546 1107 24-25 0.1246987969331812 1107 26-27 -0.7695212429159781 1107 28-29 -0.6026839408715077 1107 30-31 0.12289872252323875 1107 32-33 -0.028492044780897174 1107 34-35 -0.2536630145759631 1107 36-37 -0.2783841522627668 1107 38-39 0.3524593944402312 1107 40-41 0.018002520736402516 1107 42-43 0.08341375933263606 1107 44-45 0.7188628446156358 1107 46-47 -0.1293556341408575 1107 48-49 0.1532698195457911 1107 50-51 0.9128751509523987 1107 52-53 0.6041309408242448 1107 54-55 1.1101515632421588 1107 56-57 0.9303020190811075 1107 58-59 1.219982307192609 1107 60-61 0.5752097898074808 1107 62-63 1.6507461985868161 1107 64-65 2.1678417895744637 1107 66-67 2.4191620863686865 1107 68-69 2.0237052527778054 1107 70-71 2.4207762627375793 1107 72-73 2.671060351379694 1107 74-75 2.5437133333634456 1107 76-77 2.8291681219245532 1107 78-79 2.7555956114738756 1107 80-81 2.504649080488079 1107 82-83 1.981305865675072 1107 84-85 2.9550391184098146 1107 86-87 3.862537157449747 1107 88-89 3.827341779231613 1107 90-91 3.6487274843746356 1107 92-93 3.514959453690498 1107 94-95 3.271045198661195 1107 96-97 3.9693538010008496 1107 98-99 4.052479170307617 1107 100-101 4.695073880801235 1108 1 -0.013303453445210778 1108 2 -0.14516820089405513 1108 3 0.24722158679183082 1108 4 0.07143492398586204 1108 5 -0.03817289011855962 1108 6 -0.26133639861286895 1108 7 -0.10704277104737514 1108 8 -0.1388770810083173 1108 9 -0.1406023434498067 1108 10-11 -0.474864559854808 1108 12-13 -0.17867512775924865 1108 14-15 0.13886375075414037 1108 16-17 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0.6328184854596515 2316 62-63 1.3894107948601082 2316 64-65 0.9380281249781888 2316 66-67 0.8527024590394952 2316 68-69 1.6050717124051381 2316 70-71 1.0342855795077668 2316 72-73 0.7811535190815597 2316 74-75 0.08439656317711197 2316 76-77 0.6982675008065442 2316 78-79 0.4681732512254264 2316 80-81 0.11427640968062391 2316 82-83 0.571989095488739 2316 84-85 0.7975857022607471 2316 86-87 0.09110858602117489 2316 88-89 -0.19952157480565447 2316 90-91 0.6740380433808433 2316 92-93 0.376139577914099 2316 94-95 0.9553619688475301 2316 96-97 -0.3752114163904565 2316 98-99 -2.709477351431513 2316 100-101 -2.974180777583859 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 7.0 5 8.0 6 18.0 7 47.0 8 74.0 9 121.0 10 233.0 11 385.0 12 559.0 13 864.0 14 1045.0 15 1379.0 16 1693.0 17 2006.0 18 1954.0 19 1556.0 20 1448.0 21 1613.0 22 1607.0 23 1663.0 24 1810.0 25 1766.0 26 1306.0 27 998.0 28 1143.0 29 1247.0 30 1441.0 31 1743.0 32 2143.0 33 2797.0 34 4256.0 35 8530.0 36 7220.0 37 9213.0 38 5173.0 39 316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.53080049580583 23.673287019688104 15.18261220489464 14.613300279611426 2 15.132470377951392 27.811572058696342 35.93997751318938 21.115980050162886 3 23.611571558297417 32.004843103622235 32.95328422964383 11.430301108436513 4 10.612262546481796 25.497967772621138 33.648496728258046 30.241272952639015 5 7.6215733187282 44.341471851488855 33.49716064685365 14.539794182929288 6 20.643682799573376 40.26692802167709 14.56429621515667 24.52509296359286 7 15.42763252716843 43.46516387535672 20.698452048081634 20.408751549393216 8 22.73788590700758 31.910293736127528 20.03833847395578 25.313481882909112 9 19.72125335101323 23.017497333602375 22.942549940906865 34.31869937447753 10-11 22.250727854486755 29.795912484506065 23.567351762705023 24.386007898302154 12-13 28.418033495719353 29.45432532933614 24.824882534374908 17.3027586405696 14-15 22.728517482920644 31.283329970309303 29.18119973480153 16.80695281196852 16-17 15.24963246951659 35.687930587184 29.33685970424606 19.725577239053358 18-19 14.517454094721973 33.46617278256608 33.838747802023576 18.177625320688364 20-21 17.518953042575884 29.488916433657142 32.89325761724943 20.09887290651754 22-23 15.150903692600387 32.46663399729036 34.741719754403164 17.64074255570609 24-25 18.601902565580858 33.22787546843471 33.5521764197175 14.618045546266936 26-27 17.100369067527822 31.290727178363415 38.00458451069719 13.604319243411567 28-29 13.870412141565113 34.88177684253007 36.4845095594647 14.763301456440118 30-31 15.350523190452856 34.60623792914589 34.0758409962238 15.967397884177453 32-33 17.013548573075816 32.639089074661285 36.21720957048141 14.130152781781494 34-35 16.411627756114086 31.817035109595853 35.450528698184335 16.320808436105725 36-37 13.716559040590406 32.92781365313653 38.15656711254613 15.199060193726938 38-39 14.56313478991112 31.114346882726508 37.924124359353264 16.398393968009113 40-41 13.913338091015909 32.23834547984112 37.585692350943965 16.262624078199 42-43 14.576725679026584 31.648982180958424 35.860821175249406 17.913470964765583 44-45 14.463840399002494 30.327360788779494 36.66700301269947 18.541795799518546 46-47 17.312875654016345 30.828492771587946 35.688753080903446 16.169878493492266 48-49 15.614280360900521 29.23452768729642 36.64350984405177 18.50768210775129 50-51 11.947623302873945 30.298204145167336 39.62627194373181 18.12790060822692 52-53 14.353866997203884 31.340261162837624 36.54261912311551 17.763252716842988 54-55 13.041566977025742 29.09256002997896 36.23922631230002 21.62664668069528 56-57 16.61670173820299 29.292900175838117 33.387622149837135 20.70277593612176 58-59 16.815600588048774 30.92805050301231 34.546424144590816 17.709924764348102 60-61 15.297195237957972 34.073679052203744 33.6052578478568 17.023867861981493 62-63 12.871494047447465 35.24473206307112 32.76426162405235 19.119512265429073 64-65 12.567380588625293 37.32452221037156 31.19108702545329 18.917010175549855 66-67 14.265227292381311 34.18537949324032 29.817531924706696 21.731861289671674 68-69 13.517915309446254 33.34150067740912 31.560779452884034 21.579804560260587 70-71 16.60805396212274 32.28935458764521 30.027961142659482 21.07463030757257 72-73 15.585454440633018 29.65322417918192 33.532472399181344 21.22884898100372 74-75 14.815802369490646 32.3715084604076 30.537459283387626 22.275229886714136 76-77 15.269810613703843 29.586203914559974 31.65302239774005 23.490963073996138 78-79 15.591940272693206 29.886714133348708 32.45366233316999 22.0676832607881 80-81 16.74137384335995 29.201377878988787 31.29269839439624 22.76454988325502 82-83 15.422200762462074 28.7631250855788 33.09863722515692 22.7160369268022 84-85 15.684861413395984 29.662433877686333 31.81654391098171 22.836160797935975 86-87 15.77210227436511 30.562681963621692 30.741402669280216 22.923813092732985 88-89 15.66544636937534 30.04309475079992 28.91095673229368 25.380502147531057 90-91 16.494302990119415 28.369019220651932 30.024431199867397 25.11224658936126 92-93 17.563633218990514 29.711596667723615 30.262892392839642 22.461877720446225 94-95 16.58943666539351 28.422562210387497 30.531193473764624 24.456807650454373 96-97 14.499437894554784 29.473062177510016 31.665994061860424 24.361505866074776 98-99 16.343576143668386 29.96094087803753 28.73223602663515 24.96324695165893 100-101 15.83449846490912 28.579356270810212 29.90256136760021 25.683583896680457 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3094.0 1 1742.0 2 465.5 3 639.5 4 485.5 5 284.0 6 545.5 7 949.0 8 1134.5 9 1122.0 10 838.5 11 446.5 12 310.0 13 294.0 14 288.5 15 279.0 16 271.0 17 271.5 18 284.5 19 315.0 20 351.0 21 366.0 22 430.5 23 508.5 24 555.0 25 637.0 26 730.5 27 830.0 28 903.5 29 987.5 30 1104.5 31 1154.0 32 1188.0 33 1219.5 34 1210.0 35 1181.0 36 1156.5 37 1068.5 38 961.0 39 925.5 40 969.5 41 1112.5 42 1229.5 43 1297.0 44 1386.0 45 1435.5 46 1709.5 47 3170.0 48 6031.5 49 6415.5 50 4561.0 51 3327.5 52 1935.5 53 989.5 54 674.0 55 533.5 56 470.0 57 414.5 58 349.0 59 313.0 60 249.0 61 186.5 62 147.5 63 107.0 64 95.5 65 83.0 66 61.0 67 37.5 68 27.5 69 20.0 70 13.5 71 12.5 72 6.5 73 8.5 74 10.0 75 5.5 76 3.5 77 1.5 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.011530368107001816 3 0.007206480066876134 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002882592026750454 26-27 0.02594332824075409 28-29 7.206480066876135E-4 30-31 0.0 32-33 0.002882592026750454 34-35 0.019457496180565564 36-37 0.008647776080251362 38-39 0.026663976247441698 40-41 0.029546568274192157 42-43 0.02594332824075409 44-45 0.012971664120377044 46-47 0.004323888040125681 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0021619440200628406 84-85 0.004323888040125681 86-87 0.0 88-89 0.0 90-91 0.005044536046813294 92-93 0.0 94-95 7.206480066876135E-4 96-97 0.0 98-99 0.0 100-101 0.007206480066876134 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 69382.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.238620967974406 #Duplication Level Percentage of deduplicated Percentage of total 1 87.5008530676312 36.959153670980946 2 6.947382788507472 5.868957366463924 3 2.0371255033099023 2.5813611599550317 4 0.9861461816692828 1.6661381914617623 5 0.5050160376714666 1.066559049897668 6 0.3889988398280216 0.9858464731486553 7 0.24909574831092607 0.7365022628347411 8 0.17061352624036033 0.5765184053500908 9 0.13649082099228826 0.5188665648150818 >10 0.8155326554289225 6.367645787091752 >50 0.1057803862690234 3.238592142054135 >100 0.09895584521940899 10.688651235190685 >500 0.027298164198457656 7.021994177164107 >1k 0.03071043472326486 21.72321351359142 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3251 4.685653339482863 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1880 2.709636505145427 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1866 2.6894583609581737 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1788 2.577037271914906 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 1655 2.3853449021360005 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1277 1.8405350090801649 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1222 1.7612637283445274 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 1076 1.5508345103917442 RNA PCR Primer, Index 46 (95% over 21bp) TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1057 1.5234498861376151 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 824 1.187627915021187 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 653 0.9411662967340232 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 632 0.9108990804531435 RNA PCR Primer, Index 46 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 594 0.8561298319448848 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 551 0.7941541033697501 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 548 0.7898302153296243 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 537 0.7739759591824968 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 533 0.7682107751289959 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 461 0.6644374621659797 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 447 0.6442593179787265 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 443 0.6384941339252256 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 441 0.6356115418984751 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 441 0.6356115418984751 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 431 0.6211985817647229 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 364 0.5246317488685827 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 353 0.5087774927214551 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 316 0.45544954022657175 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 303 0.43671269205269375 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 277 0.39923899570493787 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 268 0.38626733158456084 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 242 0.34879363523680496 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 228 0.32861549104955173 RNA PCR Primer, Index 46 (96% over 26bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 217 0.3127612349024243 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 200 0.2882592026750454 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.28537661064829495 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 197 0.2839353146349197 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 192 0.2767288345680436 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 186 0.26808105848779223 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 171 0.24646161828716381 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 156 0.22484217808653542 TruSeq Adapter, Index 4 (95% over 23bp) TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT 153 0.22051829004640974 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 139 0.20034014585915655 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 131 0.18880977775215474 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 127 0.18304459369865383 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 119 0.17151422559165203 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 108 0.1556599694445245 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 107 0.1542186734311493 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 99 0.14268830532414747 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 96 0.1383644172840218 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 93 0.13404052924389612 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 93 0.13404052924389612 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 90 0.12971664120377044 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 89 0.12827534519039518 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 88 0.12683404917702 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 88 0.12683404917702 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 85 0.12251016113689431 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 83 0.11962756911014383 No Hit CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 83 0.11962756911014383 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 81 0.11674497708339339 No Hit ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC 81 0.11674497708339339 TruSeq Adapter, Index 18 (95% over 24bp) CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 78 0.11242108904326771 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 78 0.11242108904326771 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 77 0.11097979302989247 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 70 0.10089072093626587 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.001441296013375227 0.0 20-21 0.0 0.0 0.0 0.006485832060188521 0.0 22-23 0.0 0.0 0.0 0.018016200167190338 0.0 24-25 0.0 0.0 0.0 0.04323888040125681 0.0 26-27 0.0 0.0 0.0 0.09512553688276498 0.0 28-29 0.0 0.0 0.0 0.2594332824075408 0.0 30-31 0.0 0.0 0.0 0.5469718370758987 0.0 32-33 0.0 0.0 0.0 0.9656683289614021 0.0 34-35 0.0 0.0 0.0 1.558761638465308 0.0 36-37 0.0 0.0 0.0 2.3882274941627513 0.0 38-39 0.0 0.0 0.0 3.2803897264420163 0.0 40-41 0.0 0.0 0.0 4.089677437952206 0.0 42-43 0.0 0.0 0.0 4.7569974921449365 0.0 44-45 0.0 0.0 0.0 5.4034187541437255 0.0 46-47 0.0 0.0 0.0 6.056325848202704 0.0 48-49 0.0 0.0 0.0 6.623475829465856 0.0 50-51 0.0 0.0 0.0 7.20503877086276 0.0 52-53 0.0 0.0 0.0 7.679945807269897 0.0 54-55 0.0 0.0 0.0 8.197371076071605 0.0 56-57 0.0 0.0 0.0 8.856764002190769 0.0 58-59 0.0 0.0 0.0 9.328067798564469 0.0 60-61 0.0 0.0 0.0 9.818108443112045 0.0 62-63 0.0 0.0 0.0 10.218788734830358 0.0 64-65 0.0 0.0 0.0 10.669914387016807 0.0 66-67 0.0 0.0 0.0 11.16067567957107 0.0 68-69 0.0 0.0 0.0 11.755210285088351 0.0 70-71 0.0 0.0 0.0 12.349024242598944 0.0 72-73 0.0 0.0 0.0 12.807356374852267 0.0 74-75 0.0 0.0 0.0 13.339194603787725 0.0 76-77 0.0 0.0 0.0 13.78815831195411 0.0 78-79 0.0 0.0 0.0 14.38845810152489 0.0 80-81 0.0 0.0 0.0 14.963535210861608 0.0 82-83 0.0 0.0 0.0 15.443486783315556 0.0 84-85 0.0 0.0 0.0 15.965956588164076 0.0 86-87 0.0 0.0 0.0 16.45023204865815 0.0 88-89 0.0 0.0 0.0 16.97342250151336 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 35 2.7615658E-4 54.317875 1 CGCAGAA 105 4.555659E-8 36.185806 9 TACCCAT 60 0.0039142417 31.685427 2 AAGCCAC 30 0.003927676 31.685427 82-83 ACCCATG 65 0.005810441 29.227 3 GTTGATC 45 8.9144916E-4 26.385483 94-95 CATGGGG 155 1.3805457E-6 24.512966 4 GAGTACC 55 0.0028669112 21.588123 12-13 AGTACTC 155 3.8425984E-5 21.448845 5 CAGAAAA 140 1.542503E-9 20.354517 10-11 AAGGAGA 60 0.0047265575 19.803392 90-91 GAGTACT 1295 0.0 18.887524 12-13 GTACTTT 1245 0.0 18.883106 14-15 CATGGGC 155 9.509111E-4 18.384724 4 AGTACTT 1170 0.0 17.455013 12-13 CTTGAAA 580 0.0 17.196058 56-57 ACTTTTT 1385 0.0 17.145803 16-17 GCAGAAA 115 7.930885E-4 16.519608 9 TGCTTGA 615 0.0 16.217419 54-55 GCTTGAA 605 0.0 16.092964 56-57 >>END_MODULE