FastQCFastQC Report
Thu 26 May 2016
SRR938053_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938053_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70202
Sequences flagged as poor quality0
Sequence length101
%GC36

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35034.989886328024842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19712.807612318737358No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17502.492806472750064No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17182.4472237258197773No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12721.8119141904789038No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12421.76918036523176No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11411.6253098202330416No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7901.1253240648414575No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5810.8276117489530213No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC5740.817640523062021No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5690.8105182188541636No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA5490.7820290020227345No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG4860.692287969003732No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC4820.6865901256374463No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC4780.6808922822711604No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.6780433605880174No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT4220.6011224751431583No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC4210.5996980143015868No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.5840289450443007No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT4020.5726332583117291No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA3490.49713683370844136No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC3360.4786188427680123No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA3010.4287627133130111No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC2950.4202159482635822No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT2940.4187914874220108No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG2650.37748212301643824No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG2180.3105324634625794No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2160.30768354177943646No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC1940.27634540326486423No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1850.26352525569072105No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1770.2521295689581493No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT1610.22933819549300588No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC1570.22364035212672004No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG1550.2207914304435771No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA1230.17520868351329022No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1190.16951084014700435No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT1180.16808637930543288No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1100.15669069257286117No Hit
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG970.13817270163243212No Hit
GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.13674824079086065No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT950.1353237799492892No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA920.1310503974245748No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT900.12820147574143187No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG890.1267770148998604No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT880.12535255405828893No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT860.122503632375146No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA860.122503632375146No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT860.122503632375146No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT790.11253240648414575No Hit
ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA780.1111079456425743No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG770.10968348480100282No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.10541010227628843No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG730.10398564143471696No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGAA950.055.021049
GTACCCA405.139678E-447.8936771
ACCCATG405.3433573E-447.5181733
TACCCAT459.545313E-442.238382
GGGTACC350.00838041727.13390450-51
GGGCGCC601.6878163E-423.77603594-95
CAGAAAA1202.237357E-1023.7590910-11
ATCATTA500.001649614923.74216592-93
GAGTACT12900.023.2065512-13
GTACTTT12400.022.01894414-15
GTTGATC550.002846763221.61457694-95
TCATTAA550.002846763221.61457694-95
CGTATCA704.787073E-420.3794694-95
GCAGAAA1058.466468E-720.36493310-11
TATCATT704.8333633E-420.35042892-93
ACTTTTT13800.019.95718416-17
AGTACTT11500.018.59406912-13
AGTACTC1805.961556E-718.479295
GTATCAT650.00748346418.28925794-95
ATGGGTA650.00754529318.26320348-49
Produced by FastQC (version 0.11.5)