##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938053_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70202 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.176732856613775 33.0 31.0 34.0 30.0 34.0 2 32.363736075895275 34.0 31.0 34.0 31.0 34.0 3 32.34640038745335 34.0 31.0 34.0 30.0 34.0 4 35.879262699068406 37.0 35.0 37.0 35.0 37.0 5 35.88749608273269 37.0 35.0 37.0 35.0 37.0 6 36.01353237799493 37.0 35.0 37.0 35.0 37.0 7 35.975442295091305 37.0 35.0 37.0 35.0 37.0 8 36.03069713113587 37.0 35.0 37.0 35.0 37.0 9 37.58042505911512 39.0 37.0 39.0 35.0 39.0 10-11 37.61414916953933 39.0 37.0 39.0 35.0 39.0 12-13 37.57486253952879 39.0 37.0 39.0 35.0 39.0 14-15 38.527171590552975 40.0 38.0 41.0 34.0 41.0 16-17 38.46109797441668 40.0 38.0 41.0 34.5 41.0 18-19 38.513632090253836 40.0 37.5 41.0 34.5 41.0 20-21 38.50944417537962 40.0 37.5 41.0 34.5 41.0 22-23 38.31910059542463 40.0 37.0 41.0 34.5 41.0 24-25 38.1336144269394 40.0 36.5 41.0 34.0 41.0 26-27 37.84167117745933 40.0 35.5 41.0 33.5 41.0 28-29 37.599527079000595 39.5 35.0 41.0 33.0 41.0 30-31 37.143001623885354 39.0 35.0 41.0 33.0 41.0 32-33 36.47510042448933 38.0 35.0 41.0 33.0 41.0 34-35 35.965179054727784 38.0 35.0 40.0 31.0 41.0 36-37 35.563530953534084 37.0 35.0 40.0 30.5 41.0 38-39 35.08935642859178 37.0 35.0 40.0 29.5 41.0 40-41 34.942672573430954 37.0 35.0 40.0 28.5 41.0 42-43 34.47846215207544 37.0 35.0 40.0 25.0 41.0 44-45 33.92020882595938 37.0 35.0 40.0 21.0 41.0 46-47 33.16132019030797 37.0 34.0 40.0 5.0 41.0 48-49 32.440350702259195 36.0 33.0 40.0 2.0 41.0 50-51 31.695934588758156 35.0 33.0 40.0 2.0 41.0 52-53 31.18465285889291 35.0 32.5 40.0 2.0 41.0 54-55 30.808032534685623 35.0 31.5 40.0 2.0 41.0 56-57 30.186034585909233 35.0 31.0 40.0 2.0 41.0 58-59 29.606243411868608 35.0 29.0 39.5 2.0 41.0 60-61 28.796159653571124 35.0 26.5 39.0 2.0 41.0 62-63 28.180650123928093 35.0 24.5 39.0 2.0 41.0 64-65 27.436597247941656 35.0 14.0 38.0 2.0 40.0 66-67 26.47730833879377 35.0 2.0 37.0 2.0 40.0 68-69 25.61388564428364 34.5 2.0 37.0 2.0 39.0 70-71 24.827576137431983 34.0 2.0 36.0 2.0 39.0 72-73 24.368878379533346 33.5 2.0 35.0 2.0 37.5 74-75 23.980413663428394 33.0 2.0 35.0 2.0 37.0 76-77 22.941825019230222 31.5 2.0 34.5 2.0 36.0 78-79 23.428057605196432 33.0 2.0 35.0 2.0 36.0 80-81 23.217130566080737 33.0 2.0 35.0 2.0 36.0 82-83 22.902894504430073 33.0 2.0 35.0 2.0 35.0 84-85 22.593857724851144 32.0 2.0 35.0 2.0 35.0 86-87 22.357888664140624 32.0 2.0 35.0 2.0 35.0 88-89 21.95953818979516 31.5 2.0 35.0 2.0 35.0 90-91 21.674510697700917 31.0 2.0 35.0 2.0 35.0 92-93 21.418029400871767 31.0 2.0 35.0 2.0 35.0 94-95 21.15895558531096 31.0 2.0 35.0 2.0 35.0 96-97 20.713263154895873 30.5 2.0 35.0 2.0 35.0 98-99 20.25086179880915 30.0 2.0 34.0 2.0 35.0 100-101 18.77386684140053 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.09483643313050294 1101 2 -0.3670677384342298 1101 3 -0.17593080793290028 1101 4 -0.03967161591213397 1101 5 -0.036776194957312214 1101 6 -0.09671741625460584 1101 7 0.0952270072958612 1101 8 0.23902155176183726 1101 9 -0.07020970212340671 1101 10-11 -0.010918966198069313 1101 12-13 -0.1805224701576762 1101 14-15 -0.2860099316367126 1101 16-17 0.0548850496198483 1101 18-19 0.5133876344163468 1101 20-21 0.5215745296324172 1101 22-23 0.6264339109995447 1101 24-25 0.10533789711160324 1101 26-27 -0.10312297650649072 1101 28-29 0.21292865892237245 1101 30-31 0.07954268197975978 1101 32-33 -0.640122863549557 1101 34-35 0.21351761900908883 1101 36-37 0.22691046082478294 1101 38-39 0.4725455665818217 1101 40-41 -1.4773863715626874 1101 42-43 -1.252754615413913 1101 44-45 -2.2899930552023378 1101 46-47 -1.901597567852999 1101 48-49 -1.6991954690612374 1101 50-51 -0.9921235433755662 1101 52-53 -0.47608871106993433 1101 54-55 -0.4678577230485139 1101 56-57 -0.9771951528456135 1101 58-59 -0.7051116425131383 1101 60-61 0.19242090552864255 1101 62-63 0.11191756711305345 1101 64-65 0.42181604497416814 1101 66-67 -0.79401579304389 1101 68-69 -0.25514743358280256 1101 70-71 -0.3368954467354506 1101 72-73 -0.1336789102326179 1101 74-75 0.460962148734648 1101 76-77 -0.43232969332999005 1101 78-79 -0.4704298187447442 1101 80-81 -0.602035580437164 1101 82-83 -0.5285394953531153 1101 84-85 -0.8400976048628088 1101 86-87 -0.6061611720790943 1101 88-89 -0.7789865683139787 1101 90-91 -1.6913598405221784 1101 92-93 -1.9177247987849455 1101 94-95 -1.325329547822438 1101 96-97 -1.790604560474744 1101 98-99 -1.484537806580704 1101 100-101 -1.830550014929596 1102 1 0.16542629264946385 1102 2 0.20299246955701733 1102 3 0.25072491182626777 1102 4 0.2556212138634564 1102 5 0.30613568243732203 1102 6 0.26973031226208377 1102 7 0.21400095365601146 1102 8 0.2778830733819788 1102 9 0.2179676377780666 1102 10-11 0.5416261897953589 1102 12-13 0.3249509835916413 1102 14-15 1.0095565708263408 1102 16-17 0.4945402220336419 1102 18-19 0.02447137825870982 1102 20-21 0.17277868945726738 1102 22-23 0.5021865108900769 1102 24-25 -0.3544322178309258 1102 26-27 -0.3896582802831787 1102 28-29 -0.041450104077085825 1102 30-31 -0.25529585113463327 1102 32-33 -0.7954321136863953 1102 34-35 -0.8897938205092473 1102 36-37 -1.3152351330449505 1102 38-39 -0.9248271756185105 1102 40-41 -1.6511685281034687 1102 42-43 -2.2017146592015493 1102 44-45 -1.8377489391651167 1102 46-47 -0.9240387282251916 1102 48-49 -0.39172420469341773 1102 50-51 -0.7492390332496761 1102 52-53 -0.2128155857278422 1102 54-55 0.36314939244136113 1102 56-57 -0.2722143099337373 1102 58-59 -0.4834367656658465 1102 60-61 -1.1307865383684579 1102 62-63 -1.657923702728219 1102 64-65 -1.7738599265638726 1102 66-67 -1.9497355522119264 1102 68-69 -2.1039432737579524 1102 70-71 -3.2380996065603007 1102 72-73 -3.600975024080455 1102 74-75 -3.309152793794091 1102 76-77 -3.280715024473942 1102 78-79 -3.4006432834409672 1102 80-81 -3.5273229367590027 1102 82-83 -2.960531832517866 1102 84-85 -3.607202147829419 1102 86-87 -3.5603209969285743 1102 88-89 -3.0796160154951515 1102 90-91 -2.7972711705714417 1102 92-93 -3.177166506502516 1102 94-95 -3.177683132934643 1102 96-97 -4.501058857135938 1102 98-99 -3.945949957648029 1102 100-101 -3.7507744265333187 1103 1 -0.1171654325652085 1103 2 0.10749416094395059 1103 3 -0.3672818537214795 1103 4 -0.181277042706931 1103 5 0.3134216569364128 1103 6 -0.1614432500590155 1103 7 0.07374594454854133 1103 8 0.1090045930280894 1103 9 -0.327699809528724 1103 10-11 -0.07507950887755044 1103 12-13 0.17532829298534836 1103 14-15 0.233209966610886 1103 16-17 -0.24316469599914825 1103 18-19 -0.3502358816944451 1103 20-21 0.1890420253927303 1103 22-23 -0.24072267464205765 1103 24-25 -0.6877655511642544 1103 26-27 -0.11018911556810451 1103 28-29 0.07824804840795707 1103 30-31 -1.0508982450976845 1103 32-33 -0.2363636775687823 1103 34-35 -0.6209934041678409 1103 36-37 -0.6549436940649827 1103 38-39 -0.6032034441587157 1103 40-41 0.07066337405630918 1103 42-43 0.7165783410586712 1103 44-45 0.9082828068666302 1103 46-47 0.5746602048999705 1103 48-49 0.3159260685306222 1103 50-51 0.5518278415876914 1103 52-53 0.3690215206994303 1103 54-55 0.34545488294357085 1103 56-57 1.7104815006309266 1103 58-59 -0.37922130899137585 1103 60-61 0.19213825996617828 1103 62-63 -0.9700496460016979 1103 64-65 -0.8469798849297305 1103 66-67 -0.3855929552824442 1103 68-69 -0.7188274788060944 1103 70-71 -0.6068219588892099 1103 72-73 -0.4970197807809491 1103 74-75 -0.5605189140126718 1103 76-77 -0.26952584935034096 1103 78-79 -0.5091522608024022 1103 80-81 0.22131094302241294 1103 82-83 -0.0716429436289765 1103 84-85 0.5304507275283719 1103 86-87 0.25011355940762314 1103 88-89 0.7757053480229317 1103 90-91 0.429895105775163 1103 92-93 1.3083916511867884 1103 94-95 0.43173659123917574 1103 96-97 0.9363598261843862 1103 98-99 1.0770789260365916 1103 100-101 1.9926551857487524 1104 1 -0.08317314712644475 1104 2 0.08627911754954454 1104 3 -0.2971275624967973 1104 4 -0.43368784309266317 1104 5 -0.25432183390254437 1104 6 -0.25594662517956124 1104 7 -0.09645656268385494 1104 8 0.07573554770504387 1104 9 -0.2715281605412585 1104 10-11 -0.023850000680830874 1104 12-13 -0.2071451882104114 1104 14-15 -0.3559896476610547 1104 16-17 -0.17457742502518414 1104 18-19 -0.06166307552280159 1104 20-21 -0.2791354095156535 1104 22-23 -0.456730389203301 1104 24-25 -0.3936479041054284 1104 26-27 -1.0825854511515232 1104 28-29 -0.3981261078119047 1104 30-31 0.253224223561908 1104 32-33 0.3882747023733586 1104 34-35 0.3823817163721728 1104 36-37 0.5017583310073377 1104 38-39 0.9862372501517953 1104 40-41 0.509429044258809 1104 42-43 0.5035841269795966 1104 44-45 0.33475339510192015 1104 46-47 0.9759379291044041 1104 48-49 -0.27652812626204337 1104 50-51 0.7226330489165242 1104 52-53 1.004387962358063 1104 54-55 0.8219495791827214 1104 56-57 0.07909287960874778 1104 58-59 0.5438741587039004 1104 60-61 1.5861835830134297 1104 62-63 1.9648587435836404 1104 64-65 2.0960046859478005 1104 66-67 2.98362112379181 1104 68-69 2.605907333151478 1104 70-71 2.500579198294975 1104 72-73 3.2945665258728596 1104 74-75 3.453102108166693 1104 76-77 2.4074268989621004 1104 78-79 2.3279981731416655 1104 80-81 1.5952260828488356 1104 82-83 1.840121762253375 1104 84-85 1.9737969184637656 1104 86-87 1.9273073877586313 1104 88-89 1.8845529854385532 1104 90-91 1.7962162243865443 1104 92-93 1.6677214486795577 1104 94-95 1.8232505738814133 1104 96-97 1.731200713358927 1104 98-99 1.903139678206312 1104 100-101 1.1113871047458623 1105 1 0.31968517930271645 1105 2 0.1790711639455722 1105 3 0.47317069765233555 1105 4 0.23594761672507047 1105 5 0.06982895317284488 1105 6 -0.0794760369442642 1105 7 -0.10681281786198582 1105 8 -0.08540778286662487 1105 9 0.07913463979014068 1105 10-11 -0.08546971899068723 1105 12-13 -0.2524020233388313 1105 14-15 -0.09004101468674008 1105 16-17 -0.14630484838889402 1105 18-19 -0.018788179085348133 1105 20-21 -0.460577000236448 1105 22-23 -0.40246361206990855 1105 24-25 -0.2793148469389024 1105 26-27 -0.4698058322617058 1105 28-29 -1.3330888252932667 1105 30-31 -0.9366059338531727 1105 32-33 -1.1392729364004595 1105 34-35 -1.3839811668092707 1105 36-37 -2.175955007849332 1105 38-39 -3.044210140071897 1105 40-41 -2.568449994680705 1105 42-43 -2.1744398995324232 1105 44-45 -2.1903087424291456 1105 46-47 -2.5463280195285662 1105 48-49 -2.0771702140830897 1105 50-51 -2.607835053817528 1105 52-53 -2.874340289506069 1105 54-55 -2.167590603087369 1105 56-57 -1.7197886448320148 1105 58-59 -1.228909602687981 1105 60-61 -1.064499929828326 1105 62-63 -0.26836412302779067 1105 64-65 -0.057785703447393644 1105 66-67 0.027013832988163955 1105 68-69 -0.14574966767702335 1105 70-71 0.08539692820384204 1105 72-73 -0.9863986771097437 1105 74-75 -0.33966922571897484 1105 76-77 -0.016351062926879933 1105 78-79 -0.1333729950439171 1105 80-81 -0.06488162220501437 1105 82-83 -0.036431676023344295 1105 84-85 0.25072318192689735 1105 86-87 -0.3666031341966267 1105 88-89 -0.4133964760361799 1105 90-91 -1.0568285146358285 1105 92-93 -1.1627466540544944 1105 94-95 -1.1452664103770793 1105 96-97 -1.4972582758705713 1105 98-99 -0.34903513538109365 1105 100-101 -0.4394621081787484 1106 1 0.17577825952317028 1106 2 -0.021990277164860572 1106 3 -0.09572091288043083 1106 4 0.110003546506654 1106 5 0.30130476456292854 1106 6 0.03646599204124357 1106 7 0.3396048183903133 1106 8 0.23302455026258428 1106 9 0.05647695454825907 1106 10-11 0.09028043410177844 1106 12-13 0.2145300036054394 1106 14-15 -0.1850604063993302 1106 16-17 0.240917033627845 1106 18-19 0.2936225169750628 1106 20-21 0.3977614361791453 1106 22-23 -0.21589492458266335 1106 24-25 0.2760025647777766 1106 26-27 0.5865321959073029 1106 28-29 0.5903649407814413 1106 30-31 0.9707220922746131 1106 32-33 1.1203718890741285 1106 34-35 0.8320833361505251 1106 36-37 0.890078876616883 1106 38-39 0.6279678554373902 1106 40-41 0.5068714995017842 1106 42-43 -0.42704247148588337 1106 44-45 0.45900744454778675 1106 46-47 0.006948159283432176 1106 48-49 0.1662468097993397 1106 50-51 -0.23612654187631676 1106 52-53 -0.3066734187161124 1106 54-55 -1.3435448794703042 1106 56-57 -0.8350162423008882 1106 58-59 -0.36128355655612054 1106 60-61 0.02000711242519415 1106 62-63 -1.1344592444811497 1106 64-65 -0.31181713843412595 1106 66-67 -0.11985287450316306 1106 68-69 -0.009395309644769867 1106 70-71 -0.6152562663256553 1106 72-73 -0.6158128432661023 1106 74-75 -0.14973250393901694 1106 76-77 -0.1582167498017526 1106 78-79 -1.4679310681200555 1106 80-81 -1.3534098932807446 1106 82-83 -1.1658458421797029 1106 84-85 -1.1824264404450169 1106 86-87 -1.159009747791238 1106 88-89 -1.5437291969996387 1106 90-91 -1.0664223092877982 1106 92-93 -1.9866903160263263 1106 94-95 -2.083825299946376 1106 96-97 -1.6466765244927366 1106 98-99 -0.9273164172753567 1106 100-101 -0.8891457170785237 1107 1 0.2639259664916054 1107 2 0.28692375896775957 1107 3 0.230068247333989 1107 4 0.31389475158904645 1107 5 0.2072011314479738 1107 6 0.3725787575762851 1107 7 0.032874621844747764 1107 8 0.10675891595645481 1107 9 0.004660397073571687 1107 10-11 0.13197191712738743 1107 12-13 0.08022386900150735 1107 14-15 0.19155265693201784 1107 16-17 0.1178278932665151 1107 18-19 0.11742636847397847 1107 20-21 -0.14174620725940912 1107 22-23 0.1129714641407844 1107 24-25 0.29853581869875967 1107 26-27 -0.22168698217488725 1107 28-29 -0.15114786445977302 1107 30-31 -0.047642013343818235 1107 32-33 0.2454463381509555 1107 34-35 -0.23204213536029528 1107 36-37 -0.19042539179685036 1107 38-39 -1.3165427659642361 1107 40-41 -1.3999182563052486 1107 42-43 -1.7233497972750413 1107 44-45 -1.913635001352553 1107 46-47 -1.500794544707034 1107 48-49 -2.0538246613144224 1107 50-51 -1.794792414419497 1107 52-53 -1.6917240440883567 1107 54-55 -2.487579026780214 1107 56-57 -2.1177242033888355 1107 58-59 -2.069542440812622 1107 60-61 -1.3019106957939819 1107 62-63 -1.1346577753527 1107 64-65 -0.6395916073640464 1107 66-67 -1.0464957174652412 1107 68-69 -0.6650435129403824 1107 70-71 -0.3139856687477298 1107 72-73 -0.4325688488249675 1107 74-75 -0.7383765380863245 1107 76-77 -0.13568349588076956 1107 78-79 -0.10481810405414294 1107 80-81 -0.011341201599190498 1107 82-83 0.37356253582307986 1107 84-85 0.6395906332099379 1107 86-87 0.2864935279681404 1107 88-89 0.24404130131284774 1107 90-91 -0.06830835256752366 1107 92-93 0.04070694424764554 1107 94-95 -0.025258692838971086 1107 96-97 0.15767130159422038 1107 98-99 0.12221703517649729 1107 100-101 0.8691429468087115 1108 1 0.13114057836374826 1108 2 0.08235754892209712 1108 3 0.10659792769928345 1108 4 0.32101803926028083 1108 5 0.4172467935484363 1108 6 0.2538572963890715 1108 7 0.4451120647671232 1108 8 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2.5634274365608185 2316 68-69 2.595146931429813 2316 70-71 2.075005310203153 2316 72-73 1.978390055284624 2316 74-75 2.7660504582384284 2316 76-77 2.3841126952318277 2316 78-79 2.201563452912108 2316 80-81 2.212514461614976 2316 82-83 2.647869251492974 2316 84-85 1.8834095440017826 2316 86-87 1.6177664982320934 2316 88-89 1.942838494764235 2316 90-91 2.5119622789059086 2316 92-93 1.8547730986078221 2316 94-95 1.1069664992759627 2316 96-97 1.962549350374708 2316 98-99 2.30898616314175 2316 100-101 1.775841911058631 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 7.0 6 23.0 7 52.0 8 76.0 9 134.0 10 253.0 11 363.0 12 581.0 13 794.0 14 1084.0 15 1364.0 16 1726.0 17 2094.0 18 1989.0 19 1534.0 20 1462.0 21 1556.0 22 1585.0 23 1616.0 24 1729.0 25 1692.0 26 1280.0 27 855.0 28 956.0 29 1145.0 30 1376.0 31 1546.0 32 1883.0 33 2418.0 34 3536.0 35 8183.0 36 7192.0 37 9995.0 38 7417.0 39 704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.74510697700921 23.33124412409903 15.482464887040257 14.441184011851515 2 15.013034373709027 27.7283152181655 36.0660408267924 21.192609581333066 3 23.096428622305794 32.23072211063151 33.04842087268687 11.624428394375828 4 10.673485085894988 25.57761887125723 33.74405287598644 30.004843166861345 5 7.682117318594912 44.21811344406142 33.367995213811575 14.731774023532093 6 20.674624654568248 40.457536822312754 14.314406996951654 24.553431526167348 7 15.492436112931255 43.434659981197115 20.965214666248823 20.107689239622804 8 22.68168998034244 31.900800546992965 20.218797185265377 25.19871228739922 9 19.962678594322018 22.98115411461375 22.84155496360451 34.21461232745972 10-11 22.178855303267714 29.939317968149055 23.447337682687103 24.43448904589613 12-13 28.415857098088377 29.439332212757467 24.79630209965528 17.348508589498874 14-15 22.89678356741973 31.0154981339563 29.402296230876612 16.685422067747357 16-17 15.328623116150537 35.696276459360135 29.27480698555597 19.700293438933365 18-19 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31.912302070645556 35.948173315953305 17.66708692152631 44-45 14.398962955576607 30.381982777655107 36.78445003169538 18.4346042350729 46-47 17.27527189589966 30.762875437134536 35.6794370490659 16.282415617899904 48-49 15.624198740776615 29.259137916298684 36.50465798695194 18.612005355972762 50-51 12.109341614199026 30.199994302156636 39.59858693484516 18.09207714879918 52-53 14.352867439674084 31.41007378707159 36.35295290732458 17.884105865929744 54-55 12.882111620751546 29.227799777784107 36.01393122703057 21.876157374433777 56-57 16.458220563516708 29.704281929289767 33.32312469730207 20.514372809891455 58-59 16.77658756160793 31.306088145636878 34.120822768582094 17.7965015241731 60-61 15.332896498675252 34.16996666761631 33.14649155294721 17.35064528076123 62-63 12.776701518475257 35.48474402438677 32.6230021936697 19.115552263468278 64-65 12.61929859548161 37.1556365915501 31.08957009771801 19.135494715250278 66-67 14.236061650665224 34.00259251873166 30.009116549386057 21.75222928121706 68-69 13.692015127881882 33.253562958056456 31.466564104755594 21.58785780930607 70-71 16.67687530269793 32.34736902082562 30.096008660721917 20.87974701575454 72-73 15.590723910999687 29.5575624626079 33.56884419247314 21.28286943391926 74-75 14.604997008632234 32.431412210478335 30.817498076977863 22.14609270391157 76-77 15.335745420358393 29.66083587362183 31.553944332070312 23.44947437394946 78-79 15.749551294834903 29.851001395971625 32.3131819606279 22.08626534856557 80-81 16.713911284578785 29.159425657388677 31.158656448534227 22.968006609498303 82-83 15.444716674738611 29.083217002364602 33.207031138714 22.26503518418279 84-85 15.943959315659766 29.96908787874471 31.325944814028688 22.76100799156683 86-87 15.835731175749979 30.496282157203495 30.78402324720094 22.88396341984559 88-89 15.690436169909688 30.009116549386057 29.23278539072961 25.067661889974644 90-91 16.55805075390127 28.340562523592798 30.277843066031352 24.823543656474577 92-93 17.649195530010044 29.557060746565245 30.29564895336994 22.49809477005477 94-95 16.51804791886271 28.85031765476767 30.247001509928488 24.384632916441127 96-97 14.557989800860375 29.638044500156692 31.71562063758867 24.08834506139426 98-99 16.523745762228998 30.0418791487422 28.60531039001738 24.829064699011425 100-101 15.76379590325062 28.903734936326604 29.723512150650976 25.6089570097718 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3155.0 1 1749.5 2 459.5 3 651.0 4 465.5 5 276.5 6 554.5 7 931.5 8 1140.5 9 1161.0 10 818.5 11 410.5 12 321.0 13 302.5 14 285.0 15 283.0 16 278.0 17 282.0 18 296.5 19 342.0 20 392.5 21 391.0 22 435.5 23 503.5 24 571.0 25 658.5 26 735.0 27 853.5 28 950.5 29 1063.5 30 1149.0 31 1215.5 32 1259.0 33 1206.0 34 1177.0 35 1176.5 36 1125.0 37 1032.0 38 999.5 39 1000.0 40 1045.0 41 1143.0 42 1269.5 43 1372.5 44 1380.5 45 1386.5 46 1654.0 47 3211.0 48 6069.5 49 6345.5 50 4545.5 51 3369.5 52 2006.5 53 1032.0 54 691.5 55 565.5 56 466.5 57 414.5 58 372.5 59 302.0 60 249.5 61 198.0 62 149.5 63 116.5 64 94.0 65 67.5 66 51.5 67 38.5 68 26.0 69 19.0 70 13.0 71 10.5 72 11.5 73 10.0 74 7.5 75 5.5 76 4.5 77 2.5 78 1.5 79 1.5 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.004273382524714395 3 0.007122304207857325 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0014244608415714653 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.007834534628643058 26-27 0.019942451782000513 28-29 0.0 30-31 0.0 32-33 7.122304207857326E-4 34-35 0.01638129967807185 36-37 0.005697843366285861 38-39 0.015669069257286117 40-41 0.01638129967807185 42-43 0.009258995470214524 44-45 0.0035611521039286627 46-47 7.122304207857326E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 7.122304207857326E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.004273382524714395 86-87 0.0 88-89 0.0 90-91 7.122304207857326E-4 92-93 7.122304207857326E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 70202.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.155494145465944 #Duplication Level Percentage of deduplicated Percentage of total 1 87.710346691897 36.974730064670524 2 6.494559708048929 5.475627475000712 3 2.2166655403122255 2.8033389362126435 4 1.030614313712239 1.7378422267171876 5 0.5744407650199365 1.2107917153357455 6 0.3345272690410218 0.8461297398934503 7 0.23315536933162126 0.6880145864790177 8 0.19260660944786107 0.6495541437565882 9 0.13178346962222073 0.4999857553915843 >10 0.8211123876461444 6.573886783852312 >50 0.09799283638575386 2.997065610666363 >100 0.10475096303304723 10.805959944161135 >500 0.02703250658917348 6.918606307512606 >1k 0.030411569912820166 21.818466710350133 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3327 4.739181219908264 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1952 2.7805475627475 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1938 2.7606051109654994 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1764 2.5127489245320644 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 1669 2.377425144582775 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1253 1.784849434489046 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1234 1.7577846784991882 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1109 1.579727073302755 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 1071 1.5255975613230393 RNA PCR Primer, Index 46 (95% over 21bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 794 1.1310219082077433 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 660 0.940144155437167 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 616 0.8774678784080225 RNA PCR Primer, Index 46 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 591 0.8418563573687359 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 580 0.8261872881114497 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 562 0.8005469929631635 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 534 0.7606620893991625 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 520 0.740719637617162 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 460 0.655251987122874 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 455 0.6481296829150167 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 452 0.6438563003903023 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 440 0.6267627702914447 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 434 0.6182160052420159 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 421 0.5996980143015868 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 360 0.5128059029657275 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 342 0.48716560781744106 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 329 0.46864761687701206 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 301 0.4287627133130111 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 278 0.3960001139568673 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 277 0.39457565311529585 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 247 0.3518418278681519 RNA PCR Primer, Index 46 (96% over 26bp) TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 221 0.3148058459872938 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 214 0.30483462009629353 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 198 0.2820432466311501 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 197 0.28061878578957866 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 195 0.2777698641064357 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 190 0.2706475598985784 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 174 0.24785618643343496 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 159 0.22648927380986297 TruSeq Adapter, Index 4 (95% over 23bp) ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 158 0.2250648129682915 No Hit TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT 157 0.22364035212672004 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 137 0.19515113529529074 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 130 0.1851799094042905 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 118 0.16808637930543288 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 117 0.16666191846386144 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 113 0.16096407509757557 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 107 0.15241731004814676 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 103 0.14671946668186092 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 102 0.14529500584028945 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 99 0.14102162331557505 No Hit CTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 99 0.14102162331557505 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 96 0.13674824079086065 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 94 0.1338993191077177 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 92 0.1310503974245748 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 89 0.1267770148998604 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 87 0.12392809321671748 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 86 0.122503632375146 No Hit CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 82 0.11680578900886014 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 82 0.11680578900886014 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 79 0.11253240648414575 No Hit ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC 78 0.1111079456425743 TruSeq Adapter, Index 18 (95% over 24bp) GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 75 0.10683456311785988 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 71 0.10113671975157403 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 71 0.10113671975157403 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 7.122304207857326E-4 0.0 12-13 0.0 0.0 0.0 0.0014244608415714653 0.0 14-15 0.0 0.0 0.0 0.0014244608415714653 0.0 16-17 0.0 0.0 0.0 0.0014244608415714653 0.0 18-19 0.0 0.0 0.0 0.0014244608415714653 0.0 20-21 0.0 0.0 0.0 0.004985612945500128 0.0 22-23 0.0 0.0 0.0 0.01851799094042905 0.0 24-25 0.0 0.0 0.0 0.047719438192644084 0.0 26-27 0.0 0.0 0.0 0.11466909774650294 0.0 28-29 0.0 0.0 0.0 0.295575624626079 0.0 30-31 0.0 0.0 0.0 0.6025469359847297 0.0 32-33 0.0 0.0 0.0 1.100396000113957 0.0 34-35 0.0 0.0 0.0 1.7300076920885445 0.0 36-37 0.0 0.0 0.0 2.509900002848922 0.0 38-39 0.0 0.0 0.0 3.3724110424204436 0.0 40-41 0.0 0.0 0.0 4.194324948007179 0.0 42-43 0.0 0.0 0.0 4.8702316173328395 0.0 44-45 0.0 0.0 0.0 5.532605908663571 0.0 46-47 0.0 0.0 0.0 6.217771573459446 0.0 48-49 0.0 0.0 0.0 6.875160251844677 0.0 50-51 0.0 0.0 0.0 7.42713882795362 0.0 52-53 0.0 0.0 0.0 7.87441953220706 0.0 54-55 0.0 0.0 0.0 8.452750633885074 0.0 56-57 0.0 0.0 0.0 9.136491837839378 0.0 58-59 0.0 0.0 0.0 9.614398450186606 0.0 60-61 0.0 0.0 0.0 10.14073673114726 0.0 62-63 0.0 0.0 0.0 10.588729665821486 0.0 64-65 0.0 0.0 0.0 11.007521153243498 0.0 66-67 0.0 0.0 0.0 11.472607618016582 0.0 68-69 0.0 0.0 0.0 12.048802028432238 0.0 70-71 0.0 0.0 0.0 12.62285974758554 0.0 72-73 0.0 0.0 0.0 13.106464203299051 0.0 74-75 0.0 0.0 0.0 13.60217657616592 0.0 76-77 0.0 0.0 0.0 14.03948605452836 0.0 78-79 0.0 0.0 0.0 14.664824363978234 0.0 80-81 0.0 0.0 0.0 15.274493604170821 0.0 82-83 0.0 0.0 0.0 15.704680778325404 0.0 84-85 0.0 0.0 0.0 16.23956582433549 0.0 86-87 0.0 0.0 0.0 16.71960912794507 0.0 88-89 0.0 0.0 0.0 17.26588986068773 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCATGGG 20 0.0020674241 71.237175 2 ACCCATG 50 4.1897147E-7 56.989746 3 GTACCCA 60 1.4740217E-6 47.49146 1 TACCCAT 65 2.5577883E-6 43.838264 2 ACATGGA 45 9.571824E-4 42.214622 3 CGCAGAA 125 3.6379788E-12 41.792477 9 GAGTAAA 85 3.1213403E-6 22.348919 10-11 CATGGGG 180 5.0635626E-6 21.107311 4 AGTACTT 1165 0.0 19.567293 12-13 GCAGAAA 135 2.26064E-8 19.34837 10-11 AGTACTC 175 9.78227E-5 18.996582 7 GAGTACT 1325 0.0 18.638153 12-13 GTACTTT 1235 0.0 18.458216 14-15 CAGAGAA 80 0.001185388 17.809294 10-11 TACTTTT 1290 0.0 17.67124 14-15 TACATGG 695 0.0 17.083256 2 ACTTTTT 1350 0.0 16.709957 16-17 AGAGTAC 1890 0.0 16.458677 10-11 CAGAAAA 175 2.4525434E-8 16.282784 10-11 GTACATG 735 0.0 16.153555 1 >>END_MODULE