##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938052_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 68299 Sequences flagged as poor quality 0 Sequence length 101 %GC 36 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18638633069298 33.0 31.0 34.0 30.0 34.0 2 32.35993206342699 34.0 31.0 34.0 30.0 34.0 3 32.34089810978199 34.0 31.0 34.0 30.0 34.0 4 35.90013030937495 37.0 35.0 37.0 35.0 37.0 5 35.89938359273196 37.0 35.0 37.0 35.0 37.0 6 36.008989882721565 37.0 35.0 37.0 35.0 37.0 7 35.9691649950951 37.0 35.0 37.0 35.0 37.0 8 36.0102783349683 37.0 35.0 37.0 35.0 37.0 9 37.53022738253854 39.0 37.0 39.0 35.0 39.0 10-11 37.562621707492056 39.0 37.0 39.0 35.0 39.0 12-13 37.51473667257207 39.0 37.0 39.0 35.0 39.0 14-15 38.4596699805268 40.0 38.0 41.0 33.5 41.0 16-17 38.321534722323904 40.0 37.5 41.0 33.5 41.0 18-19 38.54382933864332 40.0 37.5 41.0 34.5 41.0 20-21 38.5829441133838 40.0 38.0 41.0 35.0 41.0 22-23 38.41584064188348 40.0 37.0 41.0 34.5 41.0 24-25 38.19685500519773 40.0 36.5 41.0 34.0 41.0 26-27 37.827083851886556 40.0 35.5 41.0 33.5 41.0 28-29 37.597651502950264 39.5 35.0 41.0 33.0 41.0 30-31 37.086838753129626 38.5 35.0 41.0 33.0 41.0 32-33 36.39988872457869 38.0 35.0 40.0 32.5 41.0 34-35 35.918183282332095 38.0 35.0 40.0 31.0 41.0 36-37 35.5384412656115 37.5 35.0 40.0 30.5 41.0 38-39 35.09143618501003 37.0 35.0 40.0 29.5 41.0 40-41 34.872150397516805 37.0 35.0 40.0 28.5 41.0 42-43 34.35979296915035 37.0 35.0 40.0 25.0 41.0 44-45 33.7751211584357 37.0 34.5 40.0 21.0 41.0 46-47 32.949867494399626 36.0 33.0 40.0 5.5 41.0 48-49 32.21753612790817 36.0 33.0 40.0 2.0 41.0 50-51 31.44264923351733 35.0 33.0 40.0 2.0 41.0 52-53 30.854953952473682 35.0 31.0 40.0 2.0 41.0 54-55 30.41339551091524 35.0 31.0 39.5 2.0 41.0 56-57 29.7286783115419 35.0 29.5 39.0 2.0 41.0 58-59 29.021164292302963 35.0 26.5 39.0 2.0 40.5 60-61 28.07747551208656 35.0 24.0 38.0 2.0 40.0 62-63 27.41868841417883 35.0 21.0 38.0 2.0 40.0 64-65 26.625543565791595 34.5 7.0 37.5 2.0 40.0 66-67 25.640433974143107 34.0 2.0 37.0 2.0 39.0 68-69 24.79323269740406 33.0 2.0 36.0 2.0 39.0 70-71 24.0962752016867 33.0 2.0 35.5 2.0 38.5 72-73 23.57735838006413 32.5 2.0 35.0 2.0 37.0 74-75 23.116194966251335 32.0 2.0 35.0 2.0 37.0 76-77 22.02439274367121 30.0 2.0 34.0 2.0 35.0 78-79 22.541267075652645 31.5 2.0 35.0 2.0 36.0 80-81 22.250640565747666 31.0 2.0 35.0 2.0 35.5 82-83 21.813020688443462 31.0 2.0 35.0 2.0 35.0 84-85 21.382048053412202 30.5 2.0 35.0 2.0 35.0 86-87 21.05574752192565 30.0 2.0 34.5 2.0 35.0 88-89 20.514465804770204 29.0 2.0 34.0 2.0 35.0 90-91 20.145785443417914 29.0 2.0 34.0 2.0 35.0 92-93 19.566216196430403 27.5 2.0 34.0 2.0 35.0 94-95 19.017533199607605 26.5 2.0 34.0 2.0 35.0 96-97 18.28884756731431 25.0 2.0 34.0 2.0 35.0 98-99 17.390239974230955 20.5 2.0 34.0 2.0 35.0 100-101 14.437722367823834 2.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.13467643124780437 1101 2 -0.02394743117572773 1101 3 -0.13122468854180624 1101 4 0.05463011914363136 1101 5 0.0684994818232667 1101 6 0.0556796341369008 1101 7 -0.3935330452552037 1101 8 0.301445882491052 1101 9 -0.5075131147290435 1101 10-11 -0.3087995691778076 1101 12-13 0.47779333343035546 1101 14-15 -0.33761893706981283 1101 16-17 -0.16963407746013814 1101 18-19 -0.25423931551823387 1101 20-21 -0.41181806726259396 1101 22-23 -0.22704930398828083 1101 24-25 -0.032903693676175294 1101 26-27 0.06235652404600245 1101 28-29 0.04026950203490287 1101 30-31 0.9039471015459668 1101 32-33 -0.6820485557510025 1101 34-35 -0.24615721752629582 1101 36-37 -0.010220261554579224 1101 38-39 -0.7818956864460205 1101 40-41 -0.12520139388616514 1101 42-43 0.34191268473895775 1101 44-45 0.5463157913378822 1101 46-47 0.9346695513301029 1101 48-49 0.9049551630193662 1101 50-51 1.9586319480983043 1101 52-53 1.6781731364349497 1101 54-55 1.1846430342911596 1101 56-57 2.110711295391635 1101 58-59 1.5095256321740358 1101 60-61 1.420913469021091 1101 62-63 1.4735099608418984 1101 64-65 2.7967246954741576 1101 66-67 2.096990829546609 1101 68-69 2.267842197925482 1101 70-71 2.5538699759553403 1101 72-73 2.552165649958024 1101 74-75 3.1967900849205435 1101 76-77 2.9492064709623023 1101 78-79 2.2748789798147477 1101 80-81 2.82319526797302 1101 82-83 1.919818304346105 1101 84-85 1.3416689876865142 1101 86-87 2.156313956443622 1101 88-89 2.1971376866727894 1101 90-91 2.129032988969932 1101 92-93 2.838898515190742 1101 94-95 2.581777760275113 1101 96-97 3.2528262025603567 1101 98-99 4.81424740145621 1101 100-101 4.106726995483648 1102 1 -0.022388295654586443 1102 2 0.4476022056281437 1102 3 0.382395166179748 1102 4 0.37938798839302734 1102 5 0.0684994818232667 1102 6 0.32051014261147515 1102 7 0.45604322593123925 1102 8 0.5045330495612674 1102 9 0.21766848333390953 1102 10-11 0.32164837271081836 1102 12-13 0.33372553682018236 1102 14-15 0.8456437747945955 1102 16-17 0.5264918305301833 1102 18-19 0.7726977304866054 1102 20-21 0.38191679956548796 1102 22-23 0.27673398898993185 1102 24-25 0.6760854104400451 1102 26-27 0.803730616055681 1102 28-29 0.4611230129307913 1102 30-31 0.6064289417396793 1102 32-33 0.7454635507865319 1102 34-35 0.5560522255729765 1102 36-37 0.8443499563630965 1102 38-39 0.9069662990261307 1102 40-41 0.8384789935230472 1102 42-43 1.4970276968454925 1102 44-45 0.7704380673669462 1102 46-47 0.7225024811121799 1102 48-49 1.1688776811791755 1102 50-51 0.76250604010799 1102 52-53 0.14836078776666284 1102 54-55 0.5401212425236075 1102 56-57 0.5221822397015643 1102 58-59 1.1313476660723403 1102 60-61 0.45662775473537565 1102 62-63 1.0788368373552153 1102 64-65 1.9666702160552703 1102 66-67 0.883613105575666 1102 68-69 1.8205056361821406 1102 70-71 1.33928159823137 1102 72-73 1.52447194535754 1102 74-75 1.7113179299568628 1102 76-77 1.6482076816160571 1102 78-79 1.7777542824781847 1102 80-81 0.9904168176098231 1102 82-83 1.4281415488981644 1102 84-85 1.8504462273959597 1102 86-87 1.7922098402208633 1102 88-89 1.2581243694815072 1102 90-91 0.29337923594329496 1102 92-93 0.1748549316556307 1102 94-95 0.3988177118489631 1102 96-97 1.1350901250785164 1102 98-99 1.07771592445863 1102 100-101 0.9319388598904279 1103 1 0.05699023541886561 1103 2 -0.08347124069953793 1103 3 0.2699657876486725 1103 4 0.5582015477150648 1103 5 0.018499481823269548 1103 6 0.2640129674702365 1103 7 0.248133621411462 1103 8 -0.3378398317946605 1103 9 0.3377249805090514 1103 10-11 -0.08499004536827925 1103 12-13 0.47779333343035546 1103 14-15 0.5165477295968586 1103 16-17 0.4958421130160602 1103 18-19 0.7838559225770041 1103 20-21 0.50663431368978 1103 22-23 0.5306887912498155 1103 24-25 0.41888202060953716 1103 26-27 0.15938033356980696 1103 28-29 0.4837218829872896 1103 30-31 0.6610899586888266 1103 32-33 0.9173562061537623 1103 34-35 0.5639618300927438 1103 36-37 -0.16200597584029452 1103 38-39 0.865723361173032 1103 40-41 -0.5311537748385362 1103 42-43 -0.7574920771658071 1103 44-45 -0.7292794467573529 1103 46-47 -0.6712828296222781 1103 48-49 -1.0129019798377783 1103 50-51 -1.3318442423778833 1103 52-53 -1.33313638737458 1103 54-55 -1.8492855371374084 1103 56-57 -1.3386934665131278 1103 58-59 -0.9029743678259656 1103 60-61 -0.07670557859795935 1103 62-63 0.7282718656038014 1103 64-65 -1.8110133997639402 1103 66-67 -1.4661044085486274 1103 68-69 -1.1964435163602332 1103 70-71 -0.6080347859494246 1103 72-73 -0.8924772071848324 1103 74-75 -1.059162296031836 1103 76-77 -0.6037697195138847 1103 78-79 -0.8102400678043011 1103 80-81 -0.5631142558365063 1103 82-83 -1.0164912194634148 1103 84-85 -0.3607119646944348 1103 86-87 -0.47106699593733126 1103 88-89 -1.2784575514224485 1103 90-91 -1.3221574872205473 1103 92-93 -0.8956252943330725 1103 94-95 -1.5581031921058361 1103 96-97 -3.020983321249169 1103 98-99 -2.0208716461628384 1103 100-101 -0.03910633784968631 1104 1 -0.1567352547772174 1104 2 0.17143072008477134 1104 3 0.3131030425506367 1104 4 0.34349566536211995 1104 5 0.11261712888209274 1104 6 -0.010496836451331149 1104 7 0.18734930768597025 1104 8 0.07980722702886567 1104 9 0.20047007854827115 1104 10-11 0.37922564090622757 1104 12-13 0.11014627460682647 1104 14-15 0.34988106293018717 1104 16-17 0.31986172085919407 1104 18-19 0.07454219708680654 1104 20-21 -0.0026794118004218603 1104 22-23 0.49196330105373676 1104 24-25 0.787999667668366 1104 26-27 0.3265371963149093 1104 28-29 -0.07265066603232384 1104 30-31 0.496384076335886 1104 32-33 0.331081696349834 1104 34-35 0.25856967323000646 1104 36-37 -0.1252412699579466 1104 38-39 0.013272380780868787 1104 40-41 -0.7071341669954094 1104 42-43 -0.8256293320677628 1104 44-45 -0.6537892506789191 1104 46-47 -0.4781455747203154 1104 48-49 0.4091568436916333 1104 50-51 0.6529596791907402 1104 52-53 0.17764792635091098 1104 54-55 -0.2948737724315258 1104 56-57 -0.020066015532734127 1104 58-59 -0.9059155442965512 1104 60-61 -1.1316075393822693 1104 62-63 -1.8888849971412967 1104 64-65 -0.959052615450215 1104 66-67 -0.40090833011725735 1104 68-69 -0.30673763400729115 1104 70-71 -0.2202896879102063 1104 72-73 -0.8895360307142468 1104 74-75 -0.5322015117181138 1104 76-77 -1.1542599155923163 1104 78-79 -0.5092596756474386 1104 80-81 -0.22880053034630876 1104 82-83 -0.705706905737923 1104 84-85 -0.760221768616006 1104 86-87 -0.6294003292706627 1104 88-89 -1.194143825932251 1104 90-91 -0.9623535656519202 1104 92-93 -0.72307627472523 1104 94-95 -1.1782012313215233 1104 96-97 -1.449904889876617 1104 98-99 -1.5948912540059759 1104 100-101 -2.4410671221634104 1105 1 0.09243624480853185 1105 2 0.09023767948825423 1105 3 -0.7295647287832523 1105 4 -0.07489704383423401 1105 5 -0.2695286871908209 1105 6 -0.4636865630461955 1105 7 -0.3565612142692913 1105 8 -0.26319194447071936 1105 9 0.09711465187055524 1105 10-11 -0.21045952893635445 1105 12-13 -0.5362911736119003 1105 14-15 -0.22406259904164472 1105 16-17 -0.9322095301764293 1105 18-19 -0.25499384268121617 1105 20-21 0.048066238572410214 1105 22-23 -0.10569618527600255 1105 24-25 -0.46034333150313245 1105 26-27 -0.01925816408277825 1105 28-29 -0.4153391498765657 1105 30-31 -0.3603889145506116 1105 32-33 0.17169892915845963 1105 34-35 -0.5257095314096034 1105 36-37 -0.044048229361422386 1105 38-39 0.5239393236143428 1105 40-41 -0.17751527718595383 1105 42-43 -0.5558488846775447 1105 44-45 -1.177401512485055 1105 46-47 -1.5806725009837805 1105 48-49 -1.9702963460349636 1105 50-51 -2.166116542847366 1105 52-53 -2.6287936643698835 1105 54-55 -2.549637649813466 1105 56-57 -2.3838812599403525 1105 58-59 -1.94205887486822 1105 60-61 -2.2616821044195525 1105 62-63 -2.8564699184337563 1105 64-65 -2.9549101133789613 1105 66-67 -3.0078884461073123 1105 68-69 -2.245739291008121 1105 70-71 -2.24618032585553 1105 72-73 -1.978392700142578 1105 74-75 -1.5174956293651718 1105 76-77 -1.4000138509692839 1105 78-79 -1.7131743401047679 1105 80-81 -1.3834194201557537 1105 82-83 -0.4751766654728051 1105 84-85 -0.8049607909855183 1105 86-87 -0.898766526453759 1105 88-89 -0.845241588981132 1105 90-91 -0.8782607736055255 1105 92-93 -1.208888205131192 1105 94-95 -1.15023934234058 1105 96-97 -1.1870631334557409 1105 98-99 -1.4079608480407728 1105 100-101 -2.0349983566290284 1106 1 0.4299787411659892 1106 2 0.42802301217402317 1106 3 0.36077038534982364 1106 4 0.5047532718529908 1106 5 0.43056844734050514 1106 6 0.13757618586103604 1106 7 0.2228462650896219 1106 8 0.08284982337774949 1106 9 0.7141617621182519 1106 10-11 0.46644673624091126 1106 12-13 0.4605519541200067 1106 14-15 0.47919140775777436 1106 16-17 0.5323363658896199 1106 18-19 0.8387409800482715 1106 20-21 0.8563469573679399 1106 22-23 0.3760910901003882 1106 24-25 0.8128475378509208 1106 26-27 0.961679184144522 1106 28-29 -0.01915168023110425 1106 30-31 0.5240209931715896 1106 32-33 0.9383332176480152 1106 34-35 0.7806284967594124 1106 36-37 0.5923043689872927 1106 38-39 0.4410107174948692 1106 40-41 1.143271512517778 1106 42-43 2.1051516009951143 1106 44-45 1.718421702667932 1106 46-47 1.8971079749754196 1106 48-49 1.9948566408518715 1106 50-51 1.5066615047485534 1106 52-53 1.5516337275679462 1106 54-55 1.0377834283798286 1106 56-57 0.8782605564753823 1106 58-59 0.6237497701050678 1106 60-61 0.10317947887330448 1106 62-63 -0.0067856056605677395 1106 64-65 0.193584301385485 1106 66-67 -1.9011618798129923 1106 68-69 -1.765409033601614 1106 70-71 -1.5341842112367807 1106 72-73 -1.2252358278744886 1106 74-75 -1.9140473535031006 1106 76-77 -1.7031950068702066 1106 78-79 -1.674320527574416 1106 80-81 -1.7214475891698378 1106 82-83 -1.8274107596933007 1106 84-85 -2.5776659876829413 1106 86-87 -2.461296880994798 1106 88-89 -2.117825367514399 1106 90-91 -2.0784793263010073 1106 92-93 -1.778096558700888 1106 94-95 -1.7405744564736558 1106 96-97 -1.591385620099743 1106 98-99 -1.9323658990364017 1106 100-101 -1.3911178321025588 1107 1 -0.020239811529492613 1107 2 0.17474472174177436 1107 3 0.22818175008998764 1107 4 -0.0044745086229696085 1107 5 -0.044176574514764866 1107 6 0.22645428202422124 1107 7 0.17865005333634798 1107 8 0.35652636538843296 1107 9 0.37880479271562706 1107 10-11 0.19799117528899757 1107 12-13 -0.17713624403442907 1107 14-15 0.029458527718922767 1107 16-17 -0.0871391076412209 1107 18-19 0.2661329178821674 1107 20-21 0.5198972244878988 1107 22-23 -0.028231396543610288 1107 24-25 0.08895244314475548 1107 26-27 0.26947423028342143 1107 28-29 0.3804354980107618 1107 30-31 0.12552657840713977 1107 32-33 0.7069101967640918 1107 34-35 0.4179524404213808 1107 36-37 0.10383909458224139 1107 38-39 -0.25070856370960115 1107 40-41 -1.1563885166225774 1107 42-43 -1.1685249410155762 1107 44-45 -1.0224719350202705 1107 46-47 -1.017292219293644 1107 48-49 -1.2801555009645398 1107 50-51 -1.4478066836924377 1107 52-53 -1.2133007066234072 1107 54-55 -0.8242855371374098 1107 56-57 -0.6092333726164121 1107 58-59 -0.6251574664175159 1107 60-61 -1.2687243579406804 1107 62-63 -1.328300904349252 1107 64-65 -2.102797437322625 1107 66-67 -2.6909870376566083 1107 68-69 -2.6682745022757253 1107 70-71 -1.8306873681090465 1107 72-73 -1.9995194607059616 1107 74-75 -1.728763234998972 1107 76-77 -2.090154696039704 1107 78-79 -1.6638785654568835 1107 80-81 -1.8270813919867415 1107 82-83 -1.503345679557313 1107 84-85 -1.8260875515488983 1107 86-87 -2.4339777940593947 1107 88-89 -1.774819053769864 1107 90-91 -2.1106551398027094 1107 92-93 -1.666634684004432 1107 94-95 -1.5657323000870598 1107 96-97 -1.271570175709261 1107 98-99 -1.3164115522661248 1107 100-101 -0.3307730045163524 1108 1 -0.27356532013669366 1108 2 -0.3195823518106522 1108 3 -0.2244786567957746 1108 4 -0.8501317856182737 1108 5 -0.264833851510069 1108 6 -0.2359870325297635 1108 7 0.00924473252257485 1108 8 -0.09339538735022046 1108 9 0.26828053606460855 1108 10-11 -0.22943448981272496 1108 12-13 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64-65 0.4916997785306343 2316 66-67 0.6970738860250165 2316 68-69 0.12913787898860107 2316 70-71 0.7270427334304195 2316 72-73 1.2807786067686528 2316 74-75 1.2557601845883184 2316 76-77 0.6807264045171237 2316 78-79 0.7713490794825226 2316 80-81 0.7727384573417915 2316 82-83 0.3257568425520887 2316 84-85 1.0879314461582759 2316 86-87 1.5769950195665494 2316 88-89 1.505069580360498 2316 90-91 1.6518735205313924 2316 92-93 1.1262739304731326 2316 94-95 0.05914486990966594 2316 96-97 -0.45606084062901076 2316 98-99 0.034554710426309754 2316 100-101 0.4308549024603927 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 1.0 5 8.0 6 18.0 7 45.0 8 58.0 9 124.0 10 273.0 11 378.0 12 573.0 13 817.0 14 980.0 15 1341.0 16 1616.0 17 2082.0 18 2030.0 19 1665.0 20 1529.0 21 1670.0 22 1699.0 23 1764.0 24 1878.0 25 1711.0 26 1292.0 27 1033.0 28 1208.0 29 1420.0 30 1611.0 31 1923.0 32 2304.0 33 3000.0 34 4929.0 35 8331.0 36 6927.0 37 8083.0 38 3785.0 39 191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.67828327939906 23.107785570995564 15.399821357972264 14.81410979163311 2 15.232893561565607 27.7319783872432 36.236510330487754 20.79861772070344 3 23.008668150404123 31.82324001405646 33.3943422748038 11.773749560735622 4 10.538953718209637 25.73244117776248 33.862867684739165 29.865737419288717 5 7.660882934329013 43.94758034995242 33.837030529321325 14.554506186397248 6 20.368390998140477 40.402945956630596 14.508689986383002 24.719973058845923 7 15.666408000117132 43.404735062006765 20.70015666408 20.2287002737961 8 22.701650097365995 31.55536684285275 20.19795311790802 25.54502994187323 9 19.729425028184895 23.023763159050645 22.4219973938125 34.82481441895196 10-11 22.307793671942488 29.814492159475247 23.434457312698576 24.44325685588369 12-13 28.614621004699924 29.17758678750787 24.78147557065257 17.426316637139635 14-15 22.920540564283517 30.982884083222302 29.22004714563903 16.876528206855152 16-17 15.319852995007102 35.662620612911255 29.290451996427368 19.727074395654277 18-19 14.399185932444105 33.4353358028668 33.91264879427224 18.25282947041684 20-21 17.5741958154585 29.215654694797877 32.988037892209256 20.22211159753437 22-23 15.448981683479992 32.2237514458484 34.620565454838285 17.70670141583332 24-25 18.759562801525657 33.04098918717102 33.71669948827573 14.482748523027592 26-27 17.071545715457155 31.111989691325487 38.09084519416623 13.725619399051133 28-29 13.780885098283246 34.998352794758226 36.50133606647388 14.719426040484645 30-31 15.405169952487977 34.358002005900566 34.08493597956031 16.151892062051143 32-33 17.13934318218422 32.57148713743979 36.163779850363845 14.125389830012153 34-35 16.46239676682481 31.618637614947577 35.51733731623031 16.401628301997306 36-37 13.892589935430975 32.98950204248964 38.127937451500024 14.989970570579365 38-39 14.534939053406054 31.08312895786815 37.888648925656135 16.493283063069658 40-41 13.856921139711265 32.10635743359981 37.75732817944888 16.279393247240037 42-43 14.653323279427804 31.779614486408924 35.90562018199521 17.661442052168056 44-45 14.718370693567987 30.148318423402976 36.54445892326388 18.58885195976515 46-47 17.242565776951345 30.496786190134557 35.81384793335188 16.44680009956222 48-49 15.874317339931771 29.22077922077922 36.41122124774887 18.493682191540138 50-51 12.106303536130024 30.212314225053078 39.55926495351051 18.12211728530639 52-53 14.376816687533406 31.349162767881328 36.33448283436203 17.939537710223238 54-55 13.072445729765347 29.397078742175204 35.81286378445657 21.717611743602884 56-57 16.806888825428903 29.71025635393098 32.922551970066415 20.560302850573702 58-59 17.00656858940092 31.152102754120932 34.19401137969669 17.647317276781465 60-61 15.358484211759366 34.32340818074662 33.050245627393124 17.26786198010089 62-63 12.879131822908278 35.55527892092969 32.40579370542318 19.15979555073886 64-65 12.695518453427065 37.4443468072642 30.803309900410074 19.056824838898653 66-67 14.484869341104286 34.25393733937632 29.381960359357723 21.87923296016167 68-69 13.841816184547785 33.370926400585866 31.075796411570856 21.711461003295497 70-71 16.64079546619415 32.192803901181776 29.753833086824727 21.412567545799348 72-73 15.629369861409046 29.67296048730132 33.132243445665466 21.56542620562417 74-75 14.447845124254982 32.84959069808309 30.34765035804765 22.354913819614275 76-77 15.1466334712408 29.49804122578992 31.514663347124078 23.840661955845203 78-79 15.74152728550924 29.7211400451097 32.289317203198685 22.248015466182373 80-81 16.818837449388266 29.3119732901837 31.115325196406474 22.753864064021556 82-83 15.48303464842577 28.99452919593095 33.02036721032935 22.50206894531393 84-85 15.732435104162853 29.800461318785924 31.4494929154615 23.017610661589718 86-87 15.65422442896793 30.517535572789654 30.608344135158294 23.219895863084123 88-89 15.638547158758055 29.81253661394259 29.03265963678969 25.516256590509666 90-91 16.500801862958326 28.2072687595656 29.97649333245458 25.315436045021496 92-93 17.647317276781465 29.279652018541434 30.126172570097907 22.94685813457919 94-95 16.657880092551913 28.4274961192631 30.31075183785842 24.603871950326567 96-97 14.566049893462008 29.490887523705617 31.188173184643887 24.75488939818849 98-99 16.453963023979497 29.519311733479775 28.568918176825917 25.45780706571481 100-101 16.1690194470478 28.48157802249297 29.17423266166823 26.175169868791002 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2979.0 1 1675.0 2 445.0 3 608.5 4 460.0 5 285.5 6 534.0 7 893.5 8 1131.5 9 1146.5 10 787.0 11 381.0 12 291.5 13 289.5 14 276.5 15 248.5 16 244.0 17 265.0 18 287.0 19 313.0 20 329.0 21 351.0 22 394.0 23 478.0 24 538.0 25 576.5 26 676.0 27 802.0 28 918.5 29 1044.0 30 1112.0 31 1129.0 32 1183.5 33 1229.0 34 1219.0 35 1189.0 36 1158.5 37 1070.5 38 999.5 39 990.0 40 995.5 41 1052.5 42 1200.0 43 1319.0 44 1368.0 45 1406.0 46 1713.5 47 3289.0 48 5847.5 49 6038.0 50 4437.5 51 3292.0 52 2006.5 53 1057.0 54 678.0 55 544.5 56 480.5 57 408.0 58 354.5 59 312.5 60 231.0 61 177.5 62 134.5 63 116.0 64 97.5 65 82.5 66 58.5 67 33.0 68 30.5 69 20.5 70 14.0 71 15.0 72 11.5 73 8.5 74 6.5 75 7.0 76 6.5 77 4.0 78 2.0 79 1.5 80 1.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008784901682308673 2 0.008784901682308673 3 0.004392450841154336 4 0.0 5 0.005856601121539114 6 0.002928300560769557 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.002928300560769557 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.320751401923893E-4 26-27 0.010249051962693451 28-29 0.002196225420577168 30-31 7.320751401923893E-4 32-33 0.0 34-35 0.010249051962693451 36-37 0.0 38-39 0.002196225420577168 40-41 0.0014641502803847785 42-43 7.320751401923893E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.005856601121539114 52-53 0.012445277383270618 54-55 0.009516976822501062 56-57 0.02123017906557929 58-59 0.02855093046750318 60-61 0.006588676261731504 62-63 0.026354705046926018 64-65 0.027818855327310796 66-67 0.015373577944040177 68-69 0.03513960672923469 70-71 0.017569803364617345 72-73 0.006588676261731504 74-75 0.017569803364617345 76-77 0.024158479626348848 78-79 0.030747155888080354 80-81 0.013909427663655398 82-83 0.040264132710581414 84-85 0.024158479626348848 86-87 0.0344075315890423 88-89 0.027818855327310796 90-91 0.03001508074788796 92-93 0.02855093046750318 94-95 0.019033953645002124 96-97 0.019766028785194515 98-99 0.016837728224424954 100-101 0.016105653084232566 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 68299.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.259769542745865 #Duplication Level Percentage of deduplicated Percentage of total 1 87.79059695804317 37.100103954669905 2 6.756054464192911 5.710186093500637 3 1.9090184665488688 2.420240413476039 4 0.9389183383570662 1.5871389039371002 5 0.5474136437653743 1.156678721503975 6 0.41922184111145755 1.0629731035593493 7 0.22173717215812633 0.6559393256123809 8 0.21134324221321416 0.7145053368277721 9 0.12819180265391678 0.4875620433681313 >10 0.8107265357031493 6.4905781929457245 >50 0.10740394276409243 3.243092871052285 >100 0.10393929944912171 11.48040234849705 >500 0.024252503204795068 5.992767097614899 >1k 0.031181789834736514 21.89783159343475 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 3173 4.645748839660903 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1916 2.805311937217236 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1813 2.654504458337604 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1733 2.5373724359068217 No Hit CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT 1713 2.508089430299126 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1233 1.8052972957144322 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1195 1.7496595850598107 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1100 1.6105653084232567 No Hit ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT 1080 1.581282302815561 RNA PCR Primer, Index 46 (95% over 21bp) GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 756 1.1068976119708926 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 623 0.9121656246797172 No Hit ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCT 596 0.8726335671093282 RNA PCR Primer, Index 46 (95% over 23bp) GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 543 0.7950336022489348 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 533 0.780392099445087 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 533 0.780392099445087 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 509 0.7452524927158524 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 500 0.7320751401923894 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 480 0.7027921345846937 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 443 0.648618574210457 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 435 0.6369053719673787 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 425 0.6222638691635309 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 416 0.609086516640068 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 410 0.6003016149577592 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 369 0.5402714534619834 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 338 0.4948827947700552 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 285 0.4172828299096619 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 284 0.41581867962927715 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 280 0.4099620785077381 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 277 0.4055696276665837 No Hit TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 254 0.37189417121773377 No Hit CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCT 247 0.36164511925504034 RNA PCR Primer, Index 46 (96% over 26bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 205 0.3001508074788796 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 196 0.2869734549554166 No Hit CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTG 194 0.28404515439464706 TruSeq Adapter, Index 4 (95% over 23bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 192 0.2811168538338775 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 184 0.26940365159079926 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 183 0.2679395013104145 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 175 0.2562262990673363 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 166 0.24304894654387327 No Hit TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT 153 0.22401499289887114 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 138 0.20205273869309945 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 133 0.19473198729117555 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 132 0.19326783701079078 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 127 0.18594708560886689 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 113 0.16544898168348 No Hit CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC 107 0.15666408000117132 No Hit ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGC 95 0.13909427663655397 TruSeq Adapter, Index 18 (95% over 24bp) GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 95 0.13909427663655397 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 94 0.1376301263561692 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 92 0.13470182579539963 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 92 0.13470182579539963 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 92 0.13470182579539963 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 91 0.13323767551501486 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 91 0.13323767551501486 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 83 0.12152447327193663 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 81 0.11859617271116707 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 78 0.11420372187001272 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 78 0.11420372187001272 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 72 0.10541882018770407 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 71 0.10395466990731929 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 69 0.10102636934654972 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0014641502803847788 0.0 22-23 0.0 0.0 0.0 0.014641502803847788 0.0 24-25 0.0 0.0 0.0 0.03879998243019664 0.0 26-27 0.0 0.0 0.0 0.10029429420635734 0.0 28-29 0.0 0.0 0.0 0.26208290018887537 0.0 30-31 0.0 0.0 0.0 0.5505205054246768 0.0 32-33 0.0 0.0 0.0 1.0271014216899224 0.0 34-35 0.0 0.0 0.0 1.6867011230032651 0.0 36-37 0.0 0.0 0.0 2.45098756936412 0.0 38-39 0.0 0.0 0.0 3.3624211189036446 0.0 40-41 0.0 0.0 0.0 4.2050396052650845 0.0 42-43 0.0 0.0 0.0 4.890994011625353 0.0 44-45 0.0 0.0 0.0 5.578412568266007 0.0 46-47 0.0 0.0 0.0 6.2995065813555104 0.0 48-49 0.0 0.0 0.0 6.887362918929998 0.0 50-51 0.0 0.0 0.0 7.417385320429289 0.0 52-53 0.0 0.0 0.0 7.867611531647608 0.0 54-55 0.0 0.0 0.0 8.39177733202536 0.0 56-57 0.0 0.0 0.0 9.042592131656393 0.0 58-59 0.0 0.0 0.0 9.527957949603948 0.0 60-61 0.0 0.0 0.0 9.990629438205538 0.0 62-63 0.0 0.0 0.0 10.415965094657317 0.0 64-65 0.0 0.0 0.0 10.830319624006208 0.0 66-67 0.0 0.0 0.0 11.308364690551837 0.0 68-69 0.0 0.0 0.0 11.869866323079401 0.0 70-71 0.0 0.0 0.0 12.441617007569658 0.0 72-73 0.0 0.0 0.0 12.907948871872208 0.0 74-75 0.0 0.0 0.0 13.413080718604958 0.0 76-77 0.0 0.0 0.0 13.872823906645777 0.0 78-79 0.0 0.0 0.0 14.51997833057585 0.0 80-81 0.0 0.0 0.0 15.11442334441207 0.0 82-83 0.0 0.0 0.0 15.55513257880789 0.0 84-85 0.0 0.0 0.0 16.07856630404545 0.0 86-87 0.0 0.0 0.0 16.570520798254734 0.0 88-89 0.0 0.0 0.0 17.08443754666979 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACCCA 35 2.7700956E-4 54.282784 1 CCTGAAT 20 5.372837E-4 47.497433 66-67 CGCAGAA 135 0.0 45.73827 9 ATGGGGT 55 4.915762E-5 43.17949 5 TACCCAT 45 9.5640164E-4 42.21994 2 ACCCATG 45 9.5640164E-4 42.21994 3 CTTCCGC 30 0.0039399117 31.66496 80-81 AATAGCC 35 0.008368346 27.141392 36-37 CATGGGG 160 6.0665116E-8 26.717306 4 CTCCGAC 60 1.7007024E-4 23.74872 94-95 GTTGATC 65 2.9242338E-4 21.921894 94-95 GCAGAAA 130 6.1118044E-10 21.921894 10-11 TGCTTGA 670 0.0 21.621965 54-55 CTTGAAA 650 0.0 21.55653 56-57 TGAAAAA 665 0.0 20.713167 58-59 ACATGGG 635 0.0 20.19576 3 CATGGGC 120 0.0047200453 19.7906 4 GTACATG 665 0.0 19.284674 1 GAGTACT 1160 0.0 19.24465 12-13 TCTGCTT 780 0.0 19.181656 52-53 >>END_MODULE