FastQCFastQC Report
Thu 26 May 2016
SRR938051_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938051_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3249
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG732.2468451831332716RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG351.077254539858418RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG270.8310249307479225RNA PCR Primer, Index 46 (96% over 28bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG240.7386888273314866TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA190.5847953216374269RNA PCR Primer, Index 31 (96% over 29bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150.46168051708217916No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG150.46168051708217916RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG130.40012311480455526TruSeq Adapter, Index 1 (96% over 28bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.3693444136657433No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA120.3693444136657433RNA PCR Primer, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA100.3077870113881194RNA PCR Primer, Index 46 (96% over 28bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA100.3077870113881194RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG100.3077870113881194RNA PCR Primer, Index 39 (96% over 30bp)
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC70.21545090797168356No Hit
GACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATC60.18467220683287164No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG60.18467220683287164RNA PCR Primer, Index 17 (96% over 28bp)
TCTCCGAGCCCACGAGACCAGAGAGGATCTCGTATGCCGTCTTCTGCTTG50.1538935056940597RNA PCR Primer, Index 23 (96% over 30bp)
ATCCTAGCTCATCTCCAAATAGTTGATGTATTCTTGAACCCACTTCTTCT50.1538935056940597No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA40.12311480455524776RNA PCR Primer, Index 27 (96% over 30bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA40.12311480455524776RNA PCR Primer, Index 11 (96% over 28bp)
TCCCCGGGGTGGACTCAGGGCATGGACGCGACCATCCTCCTCTTAGGAGT40.12311480455524776No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG40.12311480455524776RNA PCR Primer, Index 33 (96% over 29bp)
ACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT40.12311480455524776No Hit
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT40.12311480455524776No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA40.12311480455524776RNA PCR Primer, Index 1 (96% over 28bp)

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGCTTG250.00149868538.044-45
GTATGCC350.007793593727.14285932-33
CCACGAG350.007793593727.14285910-11
TTCTGCT350.007793593727.14285942-43
TCTCGTA350.007793593727.14285928-29
AAAAAAA1605.5100163E-411.87499952-53
Produced by FastQC (version 0.11.5)