FastQCFastQC Report
Thu 26 May 2016
SRR938050_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938050_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3176
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG642.0151133501259446RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG351.102015113350126RNA PCR Primer, Index 46 (96% over 28bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG331.0390428211586902RNA PCR Primer, Index 26 (100% over 29bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA160.5037783375314862RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG160.5037783375314862TruSeq Adapter, Index 10 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130.4093198992443325No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG120.3778337531486146RNA PCR Primer, Index 33 (96% over 29bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA110.34634760705289674RNA PCR Primer, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG110.34634760705289674TruSeq Adapter, Index 1 (96% over 28bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG110.34634760705289674RNA PCR Primer, Index 27 (96% over 30bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA90.28337531486146095RNA PCR Primer, Index 26 (100% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.28337531486146095No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC80.2518891687657431No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA70.2204030226700252RNA PCR Primer, Index 46 (96% over 28bp)
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTG70.2204030226700252RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTCTGCTTG60.1889168765743073RNA PCR Primer, Index 17 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA50.15743073047858944RNA PCR Primer, Index 27 (96% over 30bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA50.15743073047858944RNA PCR Primer, Index 33 (96% over 29bp)
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGA50.15743073047858944RNA PCR Primer, Index 39 (96% over 30bp)
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG50.15743073047858944TruSeq Adapter, Index 11 (96% over 28bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.15743073047858944No Hit
ATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTT50.15743073047858944RNA PCR Primer, Index 38 (96% over 27bp)
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTGA40.12594458438287154RNA PCR Primer, Index 11 (96% over 28bp)
ACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTT40.12594458438287154No Hit
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA40.12594458438287154No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT40.12594458438287154No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGAAA307.3893985E-539.58333646-47
TGAAAAA307.3893985E-539.58333648-49
CCCACGA500.00138333238.09
TCTCCGA500.00138333238.01
AGCCCAC550.002209967734.5454567
GCCCACG550.002209967734.5454568
GAGCCCA550.002209967734.5454566
CCGAGCC550.002209967734.5454564
TCCGAGC550.002209967734.5454563
CTCCGAG550.002209967734.5454562
TATGCCG351.8316254E-433.92857432-33
CCGTCTT351.8316254E-433.92857436-37
CGTATGC351.8316254E-433.92857430-31
CTCGTAT351.8316254E-433.92857428-29
CTTCTGC351.8316254E-433.92857440-41
GCTTGAA300.00366256431.66666846-47
CGAGCCC600.003387076531.6666685
TTGAAAA300.00366256431.66666848-49
ATCTCGT404.0117773E-429.68749826-27
TGCCGTC404.0117773E-429.68749834-35
Produced by FastQC (version 0.11.5)