FastQCFastQC Report
Thu 26 May 2016
SRR938049_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938049_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3132
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG902.8735632183908044RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG240.7662835249042145RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG220.70242656449553RNA PCR Primer, Index 46 (96% over 28bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA150.4789272030651341RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG150.4789272030651341RNA PCR Primer, Index 27 (96% over 30bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG140.4469987228607918TruSeq Adapter, Index 10 (96% over 28bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA120.38314176245210724RNA PCR Primer, Index 46 (96% over 28bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120.38314176245210724No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG110.351213282247765RNA PCR Primer, Index 33 (96% over 29bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.28735632183908044No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT90.28735632183908044No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC70.2234993614303959No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCTGCTTG70.2234993614303959TruSeq Adapter, Index 11 (96% over 28bp)
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGA60.19157088122605362RNA PCR Primer, Index 27 (96% over 30bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.19157088122605362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.19157088122605362No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA50.15964240102171137No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA50.15964240102171137RNA PCR Primer, Index 26 (100% over 29bp)
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA50.15964240102171137RNA PCR Primer, Index 10 (96% over 28bp)
CTCTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCT50.15964240102171137No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG50.15964240102171137TruSeq Adapter, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTGA50.15964240102171137RNA PCR Primer, Index 1 (96% over 28bp)
TCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGAA50.15964240102171137TruSeq Adapter, Index 4 (96% over 27bp)
CAGCTGTTCTCATCATGATCTTTATAATTTGAGAGGCCTTTGCTTCAAAA40.1277139208173691No Hit
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA40.1277139208173691No Hit
CTACAAATGTGGCTGAGGACTTTGTACATTGTTTTGTTTTTTTTTTTTTT40.1277139208173691No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC351.828181E-433.9285734-35
CCGTCTT351.828181E-433.9285736-37
GTCTTCT351.828181E-433.9285738-39
CTTGAAA351.828181E-433.9285746-47
ATCTCGT404.0042866E-429.68749826-27
TATGCCG404.0042866E-429.68749832-33
TGCTTGA404.0042866E-429.68749844-45
CGTATGC404.0042866E-429.68749830-31
CTCGTAT404.0042866E-429.68749828-29
CTTCTGC404.0042866E-429.68749840-41
ATGCCGT350.00777468527.14285534-35
GCTTGAA350.00777468527.14285546-47
CTGCTTG350.00777468527.14285544-45
GCCGTCT350.00777468527.14285536-37
CGTCTTC350.00777468527.14285538-39
TCTGCTT457.9798774E-426.38888742-43
TGAAAAA500.001476042223.7548-49
AAAAAAA1256.700154E-515.252-53
Produced by FastQC (version 0.11.5)