FastQCFastQC Report
Thu 26 May 2016
SRR938048_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938048_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTG722.1576266107281987RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTG351.0488462691039857RNA PCR Primer, Index 26 (100% over 29bp)
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTG210.6293077614623913RNA PCR Primer, Index 46 (96% over 28bp)
CTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGA200.5993407252022775RNA PCR Primer, Index 31 (96% over 29bp)
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTG150.4495055439017081RNA PCR Primer, Index 33 (96% over 29bp)
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG140.4195385076415942TruSeq Adapter, Index 10 (96% over 28bp)
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCTGCTTG130.3895714713814804TruSeq Adapter, Index 1 (96% over 28bp)
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTCTGCTTGA120.3596044351213665RNA PCR Primer, Index 46 (96% over 28bp)
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTG120.3596044351213665RNA PCR Primer, Index 27 (96% over 30bp)
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTCTGCTTGA110.3296373988612526RNA PCR Primer, Index 26 (100% over 29bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90.26970332634102484No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.23973629008091102No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80.23973629008091102No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60.17980221756068324No Hit
ACTCAGACTACCCAGATGCTTACACCACATGAAACACTGTCTCTTCTATA60.17980221756068324No Hit
TCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTTCTGCTTGAA40.11986814504045551RNA PCR Primer, Index 38 (96% over 28bp)
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA40.11986814504045551RNA PCR Primer, Index 33 (96% over 29bp)
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC40.11986814504045551No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA40.11986814504045551No Hit
ACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCACTAACAGCTGT40.11986814504045551No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT40.11986814504045551No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTCCGA458.294838E-442.222221
CGAGCCC500.001393405538.05
CCGAGCC500.001393405538.04
GCCCACG550.002225992334.5454568
CCCACGA550.002225992334.5454569
CTCCGAG550.002225992334.5454562
AGCCCAC600.003411531231.6666687
GAGCCCA600.003411531231.6666686
TCCGAGC600.003411531231.6666683
ATCTCGT458.0365746E-426.38888726-27
TCTGCTT458.0365746E-426.38888742-43
TGCTTGA458.0365746E-426.38888744-45
GTCTTCT458.0365746E-426.38888738-39
CTTGAAA458.0365746E-426.38888746-47
CTTCTGC458.0365746E-426.38888740-41
TGCCGTC500.00148645323.7534-35
TATGCCG500.00148645323.7532-33
CCGTCTT500.00148645323.7536-37
CACGAGA500.00148645323.7510-11
CTCGTAT500.00148645323.7528-29
Produced by FastQC (version 0.11.5)