##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938046_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 517631 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.3742820657959 33.0 31.0 34.0 31.0 34.0 2 32.52790887717312 34.0 31.0 34.0 31.0 34.0 3 32.44374853901718 34.0 31.0 34.0 30.0 34.0 4 35.946266742138704 37.0 35.0 37.0 35.0 37.0 5 35.99313217330492 37.0 35.0 37.0 35.0 37.0 6 36.15814354240762 37.0 36.0 37.0 35.0 37.0 7 36.135801372019834 37.0 36.0 37.0 35.0 37.0 8 36.195218215292364 37.0 37.0 37.0 35.0 37.0 9 37.77791901953322 39.0 38.0 39.0 35.0 39.0 10-11 37.76017278717851 39.0 38.0 39.0 35.0 39.0 12-13 37.677550224001266 39.0 37.5 39.0 35.0 39.0 14-15 38.49164172934001 40.0 38.0 41.0 34.0 41.0 16-17 38.477544814742544 40.0 38.0 41.0 34.5 41.0 18-19 38.73454642399702 40.0 38.0 41.0 35.0 41.0 20-21 38.858297706281114 40.0 38.0 41.0 35.0 41.0 22-23 38.826814854597195 40.0 38.0 41.0 35.0 41.0 24-25 38.69267682963346 40.0 38.0 41.0 35.0 41.0 26-27 38.19397988142132 40.0 36.5 41.0 34.5 41.0 28-29 37.90658306786108 39.5 35.5 41.0 34.0 41.0 30-31 37.550082974164994 39.0 35.0 41.0 34.0 41.0 32-33 37.11067246745268 38.0 35.0 41.0 33.0 41.0 34-35 36.83251969066768 38.0 35.0 41.0 33.0 41.0 36-37 36.516295391891134 38.0 35.0 40.5 33.0 41.0 38-39 36.18200610087108 37.5 35.0 40.0 32.5 41.0 40-41 35.89112997482763 37.5 35.0 40.0 31.5 41.0 42-43 35.78727220742189 38.0 35.0 40.0 31.0 41.0 44-45 35.48941330793558 37.5 35.0 40.0 30.5 41.0 46-47 34.916842306585195 37.0 35.0 40.0 28.0 41.0 48-49 34.44838118273442 37.0 35.0 40.0 24.0 41.0 50-51 34.203951270306455 37.0 35.0 40.0 23.5 41.0 52-53 33.51926565449133 35.0 33.5 40.0 22.5 41.0 54-55 33.3617238921162 35.0 33.0 40.0 22.0 41.0 56-57 33.35028041210824 35.0 33.5 40.0 21.0 41.0 58-59 33.13728505441135 35.0 33.0 40.0 20.0 41.0 60-61 32.70645498434213 35.0 33.0 39.5 16.5 41.0 62-63 32.16464914195633 35.0 33.0 39.0 10.5 40.0 64-65 31.52144674488197 35.0 31.5 38.0 6.0 40.0 66-67 31.043407369342255 35.0 31.0 37.5 2.0 39.5 68-69 30.719834206220263 35.0 31.0 37.0 2.0 39.0 70-71 30.144863812252357 35.0 30.5 36.0 2.0 38.5 72-73 29.606938147058425 34.5 29.5 35.5 2.0 37.5 74-75 29.116545376919078 34.0 29.0 35.0 2.0 37.0 76-77 27.64516035554285 32.5 26.5 34.5 2.0 35.5 78-79 28.318425673887383 33.5 29.0 35.0 2.0 36.0 80-81 28.411487140453332 34.0 29.0 35.0 2.0 36.0 82-83 28.134586220686167 34.0 29.0 35.0 2.0 35.5 84-85 27.75489103241498 34.0 27.5 35.0 2.0 35.0 86-87 27.219971369566352 33.5 26.0 35.0 2.0 35.0 88-89 26.451293488991194 33.0 24.5 35.0 2.0 35.0 90-91 25.87016619947414 33.0 23.5 35.0 2.0 35.0 92-93 25.52511055172507 33.0 20.0 35.0 2.0 35.0 94-95 24.999794254980863 32.5 18.0 35.0 2.0 35.0 96-97 24.16911274633861 32.0 4.5 35.0 2.0 35.0 98-99 23.305867500207675 31.5 2.0 35.0 2.0 35.0 100-101 20.46890642175604 28.0 2.0 32.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.004452919572010217 1101 2 0.021329978124413174 1101 3 0.04842005306591801 1101 4 -0.05193573240855898 1101 5 -0.03182255317228311 1101 6 0.07653160595750563 1101 7 0.022812652386718923 1101 8 0.02040080883022455 1101 9 0.10183402603782099 1101 10-11 0.17811698213800753 1101 12-13 0.11490696205915896 1101 14-15 0.13137778781380405 1101 16-17 -0.02468623707486728 1101 18-19 0.20101089782234283 1101 20-21 0.1928156279247375 1101 22-23 0.2695335319141776 1101 24-25 0.2620261699779576 1101 26-27 0.266076735421926 1101 28-29 0.18060875637814178 1101 30-31 0.23514902581357688 1101 32-33 0.3614867684628251 1101 34-35 0.20258194887208703 1101 36-37 0.5476969945253742 1101 38-39 0.7314021161542783 1101 40-41 0.641795069195453 1101 42-43 0.5194436687810935 1101 44-45 0.756024032537816 1101 46-47 0.8943550400998461 1101 48-49 0.8837051172774082 1101 50-51 0.6619800591851757 1101 52-53 0.8746653917658591 1101 54-55 0.7613038489651274 1101 56-57 0.5970637437449753 1101 58-59 0.5702601783706314 1101 60-61 0.6469404477966378 1101 62-63 0.45485502993817306 1101 64-65 0.6069082888661725 1101 66-67 0.48244140506116295 1101 68-69 0.3538365750862482 1101 70-71 0.22570258720161362 1101 72-73 0.40292554716350537 1101 74-75 0.24649056622570242 1101 76-77 0.43341052689386217 1101 78-79 0.57581535569037 1101 80-81 0.48025716400211493 1101 82-83 0.6920496285912421 1101 84-85 0.6036457449972268 1101 86-87 0.5744685713088309 1101 88-89 0.646431420696377 1101 90-91 0.7178448059925913 1101 92-93 0.4319508327059687 1101 94-95 0.4447757294176746 1101 96-97 0.8146143257507816 1101 98-99 1.1494425616640704 1101 100-101 1.3291649343866467 1102 1 0.04405251521060194 1102 2 -0.05438818957745184 1102 3 0.16992626424604396 1102 4 0.0028585222498307417 1102 5 -0.059268205346192815 1102 6 -0.13198935677541357 1102 7 -0.09683020475613802 1102 8 -0.08124903589027355 1102 9 -0.12134143980068757 1102 10-11 -0.07715281599864454 1102 12-13 -0.16066563110853593 1102 14-15 -0.04863968113029671 1102 16-17 0.031330843670481556 1102 18-19 0.0035924195614711607 1102 20-21 -0.09943980685786613 1102 22-23 -0.15714542771315365 1102 24-25 -0.23401420269285467 1102 26-27 -0.11371751923645945 1102 28-29 -0.23308495480198843 1102 30-31 -0.2638998872299041 1102 32-33 -0.5784103588663712 1102 34-35 -0.3437592778359928 1102 36-37 -0.46605494646841095 1102 38-39 -0.3499837534109389 1102 40-41 -0.3153866078231786 1102 42-43 -0.1884076510946855 1102 44-45 -0.1009635451019335 1102 46-47 -0.20391747542810634 1102 48-49 -0.19158603179091926 1102 50-51 0.05837951570691757 1102 52-53 -0.05701162686768413 1102 54-55 -0.11603504855040114 1102 56-57 -0.082517001596635 1102 58-59 -0.1366400545486215 1102 60-61 -0.3839795832592614 1102 62-63 -0.42383285826058525 1102 64-65 -0.6210711365996673 1102 66-67 -0.17462381233014312 1102 68-69 -0.09290255534853387 1102 70-71 0.12333457477925336 1102 72-73 0.042298606169715924 1102 74-75 0.13324328361700566 1102 76-77 0.23996138093112762 1102 78-79 0.24844734326801188 1102 80-81 0.09878394971640247 1102 82-83 0.0017448925663963166 1102 84-85 0.14674551207797037 1102 86-87 -0.010294627448931948 1102 88-89 0.13479516293240223 1102 90-91 -0.06722895177138355 1102 92-93 0.07925285133951121 1102 94-95 0.22848108656053512 1102 96-97 0.33221913941537906 1102 98-99 0.5206188039000956 1102 100-101 0.6214883039518639 1103 1 0.2794012470946541 1103 2 0.36880021621964687 1103 3 0.6358456483040129 1103 4 0.2542398628295359 1103 5 0.1999482801610526 1103 6 0.23166553452893623 1103 7 0.3115031285772005 1103 8 0.11601092787784495 1103 9 0.1847953355616312 1103 10-11 0.2847018035665769 1103 12-13 0.44061380729726096 1103 14-15 0.646072728290001 1103 16-17 0.5562289414965633 1103 18-19 0.5369483978223428 1103 20-21 0.4350031279247375 1103 22-23 0.4707984128665572 1103 24-25 0.5622493842636658 1103 26-27 0.4286511401838311 1103 28-29 0.3379748278067112 1103 30-31 0.2742115258135769 1103 32-33 0.4675135541771098 1103 34-35 0.6201786750625615 1103 36-37 0.6481434230968048 1103 38-39 0.412391699487614 1103 40-41 0.23889328348116834 1103 42-43 0.3682159902096629 1103 44-45 0.4178543896806701 1103 46-47 0.17411694486174412 1103 48-49 0.08869023632502859 1103 50-51 -0.18046785748149574 1103 52-53 0.2215775941468081 1103 54-55 0.22242736086989368 1103 56-57 0.056885172316405885 1103 58-59 0.24455333313253647 1103 60-61 0.29500592398711234 1103 62-63 0.42416306565245776 1103 64-65 0.45884281267569094 1103 66-67 0.07749348839449866 1103 68-69 0.43456574175291607 1103 70-71 0.3471683610111356 1103 72-73 0.5372261424016038 1103 74-75 0.3155009828923667 1103 76-77 0.22358909832243157 1103 78-79 0.6334790461665634 1103 80-81 0.5732631163830639 1103 82-83 0.8657847476388625 1103 84-85 0.8300222330924605 1103 86-87 0.9137542855945462 1103 88-89 1.5649582064106617 1103 90-91 1.706684091706876 1103 92-93 1.8116011303250161 1103 94-95 1.5675435865605323 1103 96-97 1.7407675995603036 1103 98-99 2.040625597378355 1103 100-101 2.291404517719979 1104 1 0.1386407702160426 1104 2 0.0609967480115543 1104 3 0.19074389011519344 1104 4 0.16026406803185012 1104 5 0.07071839095102916 1104 6 0.008565221426813707 1104 7 0.1537478299259476 1104 8 -0.02646558946319999 1104 9 0.1980269107061403 1104 10-11 0.22610805356657693 1104 12-13 0.16352595806797154 1104 14-15 0.20806845324182888 1104 16-17 0.2837623591266194 1104 18-19 0.225306418053556 1104 20-21 0.14744592657599043 1104 22-23 -0.01857417865559796 1104 24-25 0.02869627732724922 1104 26-27 0.04014861128209901 1104 28-29 0.06849626325950453 1104 30-31 0.08085591406020143 1104 32-33 0.3207770416530238 1104 34-35 0.25877904837662413 1104 36-37 0.2387473489156875 1104 38-39 0.34282432231997717 1104 40-41 0.3840793383944643 1104 42-43 0.2731488538898148 1104 44-45 -0.08119727216904238 1104 46-47 -0.08903936535019596 1104 48-49 -0.23655964325108414 1104 50-51 -0.0413105236664606 1104 52-53 0.048144610042761826 1104 54-55 0.043033394106885225 1104 56-57 0.01588083705629373 1104 58-59 -0.03914277717575487 1104 60-61 -0.1052830933539255 1104 62-63 -0.022014981072011608 1104 64-65 -0.06558818443413372 1104 66-67 -0.09836302653806328 1104 68-69 0.10648590071244968 1104 70-71 0.24571875840998203 1104 72-73 -0.020577325806492297 1104 74-75 0.40221371169776177 1104 76-77 0.4260969258754166 1104 78-79 0.5168334536810093 1104 80-81 0.3638610210073452 1104 82-83 0.49096538347701113 1104 84-85 0.5754084927263712 1104 86-87 0.6002607884847215 1104 88-89 0.9498931775089297 1104 90-91 0.8321657872752795 1104 92-93 1.0419193504598923 1104 94-95 1.4420167320325916 1104 96-97 1.0894173466701353 1104 98-99 1.5404374350469503 1104 100-101 1.7706840817854896 1105 1 0.16802887654449705 1105 2 0.10067723887337365 1105 3 0.21327788940433834 1105 4 0.09511263791044655 1105 5 0.1295245342063609 1105 6 0.15749987918913178 1105 7 -0.028383602814386677 1105 8 -0.019218643319568685 1105 9 -0.021510287415715368 1105 10-11 0.010574072984056215 1105 12-13 0.12750448286359983 1105 14-15 0.13701882456831527 1105 16-17 0.08695557984608371 1105 18-19 0.1409077424825398 1105 20-21 0.19041878326454054 1105 22-23 0.29263295979212245 1105 24-25 0.04512256387531011 1105 26-27 -0.19674833392620172 1105 28-29 -0.11308706539717406 1105 30-31 -0.1452211198174922 1105 32-33 0.049491709516914284 1105 34-35 0.08401615483602143 1105 36-37 0.17895349591233867 1105 38-39 0.1452674074164193 1105 40-41 -0.07274203205280827 1105 42-43 0.02244080817082761 1105 44-45 0.4037008702631937 1105 46-47 0.07497657269346547 1105 48-49 0.17133081075868262 1105 50-51 0.14782653976769922 1105 52-53 0.24921462893644986 1105 54-55 0.4747205445268463 1105 56-57 0.6638633276562089 1105 58-59 0.738760958537064 1105 60-61 0.5524492896829045 1105 62-63 0.4067707719631315 1105 64-65 0.4190723838731074 1105 66-67 0.6014948031194152 1105 68-69 0.8802928857658614 1105 70-71 0.8676148618202006 1105 72-73 0.671530349521344 1105 74-75 0.45494020214803044 1105 76-77 0.4629246565748595 1105 78-79 0.8119023875905071 1105 80-81 0.7665023963183408 1105 82-83 0.7690412201307666 1105 84-85 1.0005445834160867 1105 86-87 1.1409084118081374 1105 88-89 1.112348983109694 1105 90-91 1.15838312083309 1105 92-93 1.4827353333994502 1105 94-95 1.5384325428712131 1105 96-97 1.516083639204318 1105 98-99 1.7044833036890346 1105 100-101 1.7844895703089776 1106 1 -0.006932604084440186 1106 2 -0.05455861105887294 1106 3 -0.03412228509305493 1106 4 0.021742053715449572 1106 5 -0.02542914973059851 1106 6 -0.01763238612115714 1106 7 0.015693695817475373 1106 8 -0.035513729547268724 1106 9 -0.008356154240786395 1106 10-11 0.07505642832757076 1106 12-13 0.06324117743746882 1106 14-15 0.10499112774824937 1106 16-17 0.11713805956539858 1106 18-19 -0.008029494147066885 1106 20-21 0.03916481530523441 1106 22-23 0.030619556907346634 1106 24-25 0.12915605252370455 1106 26-27 -0.08986682508066934 1106 28-29 0.05514141480480106 1106 30-31 0.012843815488530197 1106 32-33 0.16439452932051069 1106 34-35 -0.030890377678041148 1106 36-37 -0.11728738474258193 1106 38-39 0.046919563573020184 1106 40-41 0.04519407220009697 1106 42-43 0.1051080193683589 1106 44-45 0.30556650727149304 1106 46-47 -0.08133039420771837 1106 48-49 0.27096696513318363 1106 50-51 0.1347782188410065 1106 52-53 -0.11638896496727824 1106 54-55 -0.08185930659345786 1106 56-57 0.2955921512838984 1106 58-59 0.5629214218833312 1106 60-61 0.5644450571929767 1106 62-63 0.6064273462451908 1106 64-65 0.497317756907691 1106 66-67 0.3138228582160387 1106 68-69 -0.058926331223503325 1106 70-71 -0.12001067420237632 1106 72-73 0.08662066120338707 1106 74-75 0.40861114461958437 1106 76-77 0.3267350113243417 1106 78-79 0.44520725681028495 1106 80-81 0.6796156896781582 1106 82-83 0.613892618894436 1106 84-85 0.8097391108808658 1106 86-87 0.5401584442943559 1106 88-89 1.0356202522997613 1106 90-91 0.8015693689535297 1106 92-93 0.7292611645825069 1106 94-95 0.9398099584534592 1106 96-97 0.6807497737158208 1106 98-99 0.9343502030188908 1106 100-101 1.0754454076180053 1107 1 0.06270819675069106 1107 2 0.061889647113957835 1107 3 0.1811425135322864 1107 4 0.19297598696962837 1107 5 0.09511224982959021 1107 6 0.02647381314056929 1107 7 0.007015949090018125 1107 8 -0.0012547459808160966 1107 9 -0.00231868101122501 1107 10-11 -0.020608644369630724 1107 12-13 -0.06550085420993668 1107 14-15 0.04543609992852282 1107 16-17 0.15710986316636166 1107 18-19 0.09093233309438631 1107 20-21 0.10061933336563555 1107 22-23 -0.13386661214907747 1107 24-25 -0.06578473862563783 1107 26-27 -0.023628604969395894 1107 28-29 0.19617721992678838 1107 30-31 0.14465301268037223 1107 32-33 0.054814210837527355 1107 34-35 0.0857211039243495 1107 36-37 0.36403519138949036 1107 38-39 0.2632677352912438 1107 40-41 0.17989644952244532 1107 42-43 0.02577784527532856 1107 44-45 0.19454587654746547 1107 46-47 0.4060099248492435 1107 48-49 0.14200969379844253 1107 50-51 0.15818374117392153 1107 52-53 0.3662896211949658 1107 54-55 0.3566054604633848 1107 56-57 0.1988293561813279 1107 58-59 0.2879276100243402 1107 60-61 0.6009784067888688 1107 62-63 0.3740707227819158 1107 64-65 0.3755925390671848 1107 66-67 0.5240689847985003 1107 68-69 0.27105045876354694 1107 70-71 0.5670738683594791 1107 72-73 0.29176022620398 1107 74-75 0.4481058335802999 1107 76-77 0.4389825082661467 1107 78-79 0.2877307269045204 1107 80-81 0.11571640750251433 1107 82-83 0.4009434405719432 1107 84-85 0.4664820321856489 1107 86-87 0.7456316308103048 1107 88-89 1.0243273433712616 1107 90-91 1.095755386265978 1107 92-93 1.0141129541211384 1107 94-95 0.9278713304629704 1107 96-97 1.083195972762308 1107 98-99 1.1639033295547172 1107 100-101 1.2221204925479974 1108 1 -0.0084608192002662 1108 2 -0.04229024226857803 1108 3 0.08967735524329612 1108 4 -0.018403801978394085 1108 5 -0.050758426778230614 1108 6 0.01724290441741516 1108 7 -0.027871096949446894 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#Quality Count 3 7.0 4 19.0 5 36.0 6 79.0 7 178.0 8 283.0 9 413.0 10 705.0 11 1080.0 12 1876.0 13 2710.0 14 3395.0 15 4342.0 16 5853.0 17 8319.0 18 8133.0 19 5292.0 20 4325.0 21 4261.0 22 4729.0 23 5232.0 24 5630.0 25 6528.0 26 7081.0 27 7969.0 28 9557.0 29 11543.0 30 13583.0 31 16639.0 32 20708.0 33 27332.0 34 43126.0 35 99562.0 36 74857.0 37 72868.0 38 37578.0 39 1803.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.83925808152912 17.778881094833963 13.848475072010757 13.53338575162616 2 13.953784922137642 26.20619034738591 39.54132694462692 20.29869778584953 3 24.19846871057548 35.11842087468581 30.032669826102243 10.650440588636464 4 10.59461276469145 19.222959985008625 37.495435937955804 32.68699131234412 5 9.4615662508621 45.33866789276531 30.999688967623655 14.200076888748935 6 20.822015725518227 38.628363889264534 19.20734887854259 21.342271506674653 7 21.36541281337478 44.28869213783564 18.309761200546333 16.036133848243246 8 23.44265873820825 30.72470823698054 20.124065691837203 25.708567332974003 9 28.54156725543872 22.047945351031913 20.677471016998595 28.733016376530774 10-11 22.67271473308206 28.891333787968648 23.505933763626985 24.930017715322307 12-13 33.58203044253532 28.800149140990396 23.179059986747315 14.438760429726969 14-15 28.63680884645626 28.455791867179514 30.382453910217897 12.524945376146329 16-17 15.256345984249384 33.646587672094284 33.06035869215589 18.036707651500443 18-19 14.101357917126291 34.30484263886823 37.54527839329561 14.04852105070987 20-21 16.868773315353987 31.63334498899795 34.363668327437885 17.134213368210172 22-23 15.436189099957307 31.173364810067405 32.83168898307868 20.55875710689661 24-25 16.789134124324672 32.31421475772308 35.5501762391559 15.346474878796338 26-27 19.629075703647022 29.023531548661108 37.13764545387793 14.209747293813935 28-29 14.952847639387556 29.97456680006298 40.87500639935243 14.197579161197032 30-31 13.974626712851432 36.94349836080142 37.16392565360266 11.917949272744483 32-33 13.047648762921154 36.16116751881965 35.00305731819582 15.788126400063367 34-35 14.293084259502573 32.19185228695531 35.6196767811046 17.895386672437514 36-37 17.490837360000462 32.440285862775816 36.89592747941908 13.172949297804637 38-39 20.08749403405852 28.933415262375828 38.20386032644086 12.775230377124794 40-41 14.06531997202008 32.041741865920535 41.21675935337015 12.676178808689235 42-43 12.697014138064958 36.189833036737326 37.414475044392795 13.698677780804925 44-45 13.15424921461113 37.13788066726175 33.92464517425393 15.783224943873195 46-47 13.050500367064641 34.20124801978285 31.772052857308452 20.976198755844056 48-49 15.399015320774181 33.244949824247215 33.61780266039192 17.73823219458668 50-51 18.65073768765781 34.191441393579595 33.7936676899181 13.364153228844486 52-53 13.515515879072156 34.23104489491549 37.27404270609759 14.979396519914765 54-55 11.223535684686583 33.79183240570986 37.973672365063145 17.010959544540416 56-57 14.627215139742402 37.22555256543754 31.699511814400605 16.447720480419452 58-59 12.976425291375518 33.87770438787476 33.84843643444847 19.297433886301246 60-61 13.972791428643184 31.408957346063122 35.29270851243454 19.32554271285916 62-63 19.9434539276048 30.74709590422521 33.419559493152455 15.889890675017531 64-65 17.615637394205525 31.665510759595154 36.71292870790196 14.005923138297357 66-67 13.541384932808889 32.9257690851345 37.63310717349139 15.899738808565225 68-69 12.814003852179843 36.929538337303356 35.57248531283989 14.683972497676908 70-71 13.402790791123406 35.780314548394514 31.97238959799548 18.844505062486597 72-73 13.317401778486992 33.61825315717181 30.993217175941933 22.071127888399264 74-75 15.119071307553064 33.597002497918396 33.37734795636273 17.9065782381658 76-77 17.43925692240225 30.40235225479154 35.79644573064596 16.361945092160248 78-79 14.159893823978859 30.530725555463256 38.70981452038228 16.599566100175608 80-81 12.977827749218068 36.293658199212175 35.42672454595859 15.301789505611163 82-83 12.802897850760687 35.35078483458102 32.825307896643324 19.021009418014973 84-85 13.952133753470283 31.627154860135743 33.11592340936885 21.30478797702513 86-87 16.418804310448085 31.668811072086793 34.64422188993598 17.268162727529145 88-89 18.565348675021394 28.49046907932485 35.929648726602544 17.014533519051216 90-91 14.329265936647529 30.689493288409082 38.3831904979019 16.59805027704149 92-93 12.46545557110719 34.67618310744827 35.630918193576306 17.227443127868238 94-95 12.370998589726252 35.778548383980834 32.92332360952804 18.92712941676487 96-97 13.037111450263703 32.85020188165292 30.593474103123853 23.519212564959528 98-99 15.247280876301605 31.69377740857369 32.07648320228735 20.982458512837354 100-101 17.552502470448797 32.27830921808243 32.72728853392991 17.441899777538865 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12750.0 1 7767.0 2 4631.5 3 6051.5 4 4182.5 5 3585.0 6 7859.0 7 16363.5 8 21756.5 9 20147.5 10 13447.5 11 7478.5 12 6149.0 13 5705.5 14 4835.0 15 3516.0 16 2317.5 17 1850.5 18 1749.0 19 1593.5 20 1481.0 21 1492.0 22 1521.5 23 1578.0 24 1686.0 25 1770.0 26 2042.0 27 2308.0 28 2381.5 29 2743.5 30 3327.0 31 3849.5 32 4783.0 33 5901.0 34 6853.5 35 7753.0 36 9351.0 37 10863.0 38 10974.0 39 10554.5 40 9637.5 41 8274.0 42 7129.5 43 6916.0 44 8177.5 45 8540.0 46 9801.0 47 17301.0 48 66886.5 49 70326.0 50 27726.0 51 17552.5 52 6412.5 53 2680.0 54 1768.0 55 1435.5 56 1207.5 57 969.0 58 795.5 59 715.0 60 648.0 61 576.5 62 441.5 63 258.0 64 136.5 65 114.0 66 113.0 67 85.5 68 51.0 69 49.0 70 57.0 71 46.5 72 41.5 73 57.0 74 48.5 75 26.5 76 22.0 77 16.5 78 9.5 79 5.0 80 2.0 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.009852578381124778 3 0.005409258719048898 4 0.0 5 0.0 6 1.931878113946035E-4 7 0.0 8 3.86375622789207E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 9.659390569730174E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.002028472019643337 26-27 0.021830222687590193 28-29 9.659390569730174E-5 30-31 0.0 32-33 0.003960350133589372 34-35 0.016807339591330503 36-37 0.00772751245578414 38-39 0.021250659253406384 40-41 0.022602973933168607 42-43 0.017773278648303522 44-45 0.010238954003913985 46-47 0.0021250659253406385 48-49 9.659390569730174E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.795634341838104E-4 68-69 3.86375622789207E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.86375622789207E-4 82-83 0.0011591268683676208 84-85 0.004250131850681277 86-87 9.659390569730175E-4 88-89 0.0 90-91 0.003670568416497466 92-93 9.659390569730174E-5 94-95 1.931878113946035E-4 96-97 1.931878113946035E-4 98-99 1.931878113946035E-4 100-101 0.0024148476424325437 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 517631.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.30319281495892 #Duplication Level Percentage of deduplicated Percentage of total 1 73.25290522782686 13.407620486408273 2 11.143831206527132 4.079353825408448 3 4.6441425751770575 2.550079110408766 4 2.526835755675881 1.8499664819147232 5 1.515679258625967 1.387088485813253 6 1.0533759750060692 1.1568086146308858 7 0.8074475159114658 1.0345207300181019 8 0.6005720739263058 0.8793909174682351 9 0.4264167273571662 0.7024308822307782 >10 3.3532820366676166 12.085056729600815 >50 0.36519848432074137 4.582028510657206 >100 0.2332626157077568 8.691906010265999 >500 0.028498147620404674 3.7464912263755457 >1k 0.03588655626273181 15.246961638696291 >5k 0.005277434744519384 6.506372299958851 >10k+ 0.007388408642327137 22.09392405014383 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 21248 4.104854616512535 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19918 3.8479148273577124 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 17768 3.4325610328593146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16763 3.238407282407738 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13552 2.6180812200196666 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12600 2.434166423572004 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12516 2.417938647414857 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8111 1.5669463382216289 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7187 1.3884408004930153 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6848 1.3229501324302446 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6017 1.1624110611613292 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5516 1.065623967652633 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 4943 0.9549273517235249 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4714 0.9106873429141609 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4379 0.8459694260969687 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4103 0.792649590152058 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4005 0.773717184635387 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3472 0.6707480811620634 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3416 0.6599295637239655 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3404 0.6576113099872303 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3329 0.643122224132635 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3320 0.6413835338300836 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 3063 0.5917342663016705 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3029 0.5851658807142539 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2945 0.5689381045571074 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2920 0.5641084092722422 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2135 0.4124559773274784 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2045 0.39506907430196414 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1955 0.3776821712764498 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1667 0.322044081594804 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1664 0.3214645181606202 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1661 0.3208849547264364 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1613 0.3116119397794954 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1358 0.26234904787387153 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1342 0.2592580428915579 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1328 0.2565534135320334 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1290 0.2492122766990385 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1163 0.22467742465192386 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1142 0.2206204806126372 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1115 0.2154044097049829 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1112 0.2148248462707991 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1098 0.21212021691127464 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1074 0.20748370943780414 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1069 0.2065177703808311 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1031 0.19917663354783618 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1019 0.19685837981110094 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 980 0.1893240551667114 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 951 0.1837216086362679 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 929 0.17947147678558664 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 923 0.17831234991721903 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 878 0.16961889840446187 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 850 0.16420963968541297 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 820 0.15841400534357486 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 810 0.15648212722962881 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 804 0.1553230003612612 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 798 0.15416387349289357 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 792 0.15300474662452598 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 790 0.15261837100173675 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 760 0.14682273665989864 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 725 0.14006116326108753 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 708 0.13677697046737927 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 670 0.12943583363438435 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 665 0.12846989457741131 No Hit GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA 638 0.12325382366975703 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 636 0.12286744804696782 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 583 0.11262849404305383 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 566 0.10934430124934559 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 538 0.10393504253029667 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 528 0.10200316441635064 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 521 0.10065084973658843 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 519 0.10026447411379921 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 3.86375622789207E-4 0.0 14-15 0.0 0.0 0.0 3.86375622789207E-4 0.0 16-17 0.0 0.0 0.0 8.693451512757157E-4 0.0 18-19 0.0 0.0 0.0 0.001159126868367621 0.0 20-21 0.0 0.0 0.0 0.0030910049823136563 0.0 22-23 0.0 0.0 0.0 0.008596857607059855 0.0 24-25 0.0 0.0 0.0 0.018739217705276538 0.0 26-27 0.0 0.0 0.0 0.03255214621999069 0.0 28-29 0.0 0.0 0.0 0.06143372402348391 0.0 30-31 0.0 0.0 0.0 0.1216117272729029 0.0 32-33 0.0 0.0 0.0 0.20893261802326368 0.0 34-35 0.0 0.0 0.0 0.35082906549259996 0.0 36-37 0.0 0.0 0.0 0.5412156536219817 0.0 38-39 0.0 0.0 0.0 0.7440628555863154 0.0 40-41 0.0 0.0 0.0 0.9661322447844121 0.0 42-43 0.0 0.0 0.0 1.1481151631181286 0.0 44-45 0.0 0.0 0.0 1.327490045998018 0.0 46-47 0.0 0.0 0.0 1.568588434618483 0.0 48-49 0.0 0.0 0.0 1.858273557804691 0.0 50-51 0.0 0.0 0.0 2.090098931478215 0.0 52-53 0.0 9.659390569730174E-5 0.0 2.2912074431399976 0.0 54-55 0.0 1.931878113946035E-4 0.0 2.487003289988428 0.0 56-57 0.0 1.931878113946035E-4 0.0 2.675264812192469 0.0 58-59 0.0 1.931878113946035E-4 0.0 2.9795356151389694 0.0 60-61 0.0 1.931878113946035E-4 0.0 3.4427033929575317 0.0 62-63 0.0 1.931878113946035E-4 0.0 3.767162322194768 0.0 64-65 0.0 1.931878113946035E-4 0.0 3.9768676914636103 0.0 66-67 0.0 1.931878113946035E-4 0.0 4.219125206952443 0.0 68-69 0.0 1.931878113946035E-4 0.0 4.472104645973676 0.0 70-71 0.0 1.931878113946035E-4 0.0 4.797432920362189 0.0 72-73 0.0 1.931878113946035E-4 0.0 5.170285396353773 0.0 74-75 0.0 1.931878113946035E-4 0.0 5.501119523367032 0.0 76-77 0.0 1.931878113946035E-4 0.0 5.77003695682832 0.0 78-79 0.0 1.931878113946035E-4 0.0 6.052670724898625 0.0 80-81 0.0 1.931878113946035E-4 0.0 6.3640894768667255 0.0 82-83 0.0 1.931878113946035E-4 0.0 6.683718710819097 0.0 84-85 0.0 1.931878113946035E-4 0.0 7.240872358881134 0.0 86-87 0.0 1.931878113946035E-4 0.0 7.86390305062873 0.0 88-89 0.0 1.931878113946035E-4 0.0 8.237431683960196 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 25 5.2721676E-5 76.00123 2 GAGGCGC 20 0.002074092 71.24427 6 GTCAACG 25 0.005021318 56.995422 3 ATCACGC 35 2.7876138E-4 54.281353 3 CGTGTAC 40 5.3849875E-4 47.50077 2 AGGGTCT 40 5.387553E-4 47.49618 5 TCACGCA 45 9.6239697E-4 42.218826 4 ATGGGCG 60 8.2883584E-5 39.580154 5 GGGCTGA 160 0.0 38.59065 7 GAGATGA 75 6.918728E-6 37.99695 9 ATGGGTA 180 0.0 36.941475 5 TGAGATG 65 1.3278033E-4 36.535526 4 GGAGATG 80 1.0770576E-5 35.622135 8 TGGGTAT 80 1.0770576E-5 35.622135 6 TGTAGTA 55 0.0025793375 34.54268 7 TACTATC 55 0.0025793375 34.54268 5 GGGATAT 55 0.0025793375 34.54268 9 CGTATAG 70 2.0515818E-4 33.929127 1 GGGAGAT 70 2.0527467E-4 33.925846 7 ATGGGTT 175 3.6379788E-12 32.56881 5 >>END_MODULE