FastQCFastQC Report
Thu 26 May 2016
SRR938045_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938045_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences520101
Sequences flagged as poor quality0
Sequence length101
%GC33

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA222534.278592042699398No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207243.9846106813868847No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA175003.364731081078483No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT161783.11054968169644No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT133892.5743076825462747No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA131572.5297009619285484No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122912.363194841002036No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG78481.508937687103082No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT68321.3135910140530398No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA66101.2709069969102154No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC55481.066715887875624No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55371.064600914053232No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG52941.0178792196131137No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44720.8598329939761701No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43280.8321460639375814No Hit
TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG43010.826954764555346No Hit
GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT40780.7840784770650316No Hit
ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA34250.6585259401539316No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34190.6573723180689904No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA32380.6225713851732645No Hit
CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG32250.6200718706558919No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31970.6146883009261662No Hit
ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG29160.5606603332814203No Hit
ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT28440.5468168682621259No Hit
GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA27950.5373956212351063No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT27540.529512536988008No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20460.3933851309649472No Hit
GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT19490.37473490725839786No Hit
GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC18050.34704797721980923No Hit
CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA16840.32378326517349515No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG16080.30917071876424No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16050.3085939077217694No Hit
TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT14300.2749465969109846No Hit
GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13780.2649485388414943No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13100.2518741552121607No Hit
AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC12720.24456788200753315No Hit
ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT12080.2322625797681604No Hit
GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT11960.22995533559827802No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC11630.22361041413110147No Hit
GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT11470.22053408857125828No Hit
GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC11070.21284327467165032No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC10660.20496019042455216No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG10650.20476792007706196No Hit
GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10640.20457564972957176No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC10640.20457564972957176No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9710.18669450741298327No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG9460.18188774872572827No Hit
GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9180.1765041789960027No Hit
AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG8950.17208196100372813No Hit
GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT8500.16342979536666916No Hit
GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA8320.15996892911184557No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC8230.1582384959844338No Hit
CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT7990.15362400764466902No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7950.15285492625470823No Hit
TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA7450.14324140888019826No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG7390.14208778679525708No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7260.13958827227788448No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT7040.13535832463310013No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7010.1347815135906295No Hit
GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA6960.13382016185317852No Hit
GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6850.13170518803078635No Hit
GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA6660.12805205142847256No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6630.12747524038600194No Hit
TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA6340.12189940030878617No Hit
GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5890.11324723467172722No Hit
GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA5840.11228588293427623No Hit
ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA5740.11036317945937425No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5500.10574869111960945No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5270.10132647312733488No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5240.10074966208486427No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCACT255.1101335E-576.481381
GGAGCTC250.00500748557.0351838
GGTAATC250.00500748557.0351838
GCTGTGC250.00500748557.0351838
GTCTATG250.005011303457.0242042
TGGCCGT205.3971226E-447.483690-91
GGGAACC853.6152778E-739.145567
GTACCCA500.001565664538.240691
GCGTATC500.001565664538.240691
TACCTGG1700.036.338952
GATGGAA801.0719292E-535.6469889
AGTACTC2300.035.1236045
GGACCCG702.0443642E-433.9495129
ATGGGTT1850.033.392555
TGGGGAC1001.2930795E-633.264126
GTCACTA600.003828761431.867241
GGCTTCG753.0632984E-431.6892627
ACTCAAT600.003937065631.6862138
AGTACCC600.00394077931.6801095
AAAGTAC2150.030.9433673
Produced by FastQC (version 0.11.5)