##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938045_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 520101 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96287451860312 33.0 31.0 34.0 31.0 34.0 2 31.98852722836526 33.0 31.0 34.0 30.0 34.0 3 31.98639687291507 34.0 31.0 34.0 30.0 34.0 4 35.524290474350174 37.0 35.0 37.0 33.0 37.0 5 35.603434717487566 37.0 35.0 37.0 35.0 37.0 6 35.752611511994786 37.0 35.0 37.0 35.0 37.0 7 35.75223658481718 37.0 35.0 37.0 35.0 37.0 8 35.7831152026241 37.0 36.0 37.0 35.0 37.0 9 37.34291801015572 39.0 37.0 39.0 35.0 39.0 10-11 37.28128286621252 39.0 37.0 39.0 34.5 39.0 12-13 37.15337982430336 39.0 37.0 39.0 34.0 39.0 14-15 37.998159972774516 40.0 38.0 41.0 33.0 41.0 16-17 37.99311768291159 40.0 37.5 41.0 33.5 41.0 18-19 38.248542110090156 40.0 38.0 41.0 34.0 41.0 20-21 38.26616849419632 40.0 38.0 41.0 34.0 41.0 22-23 38.1169609364335 40.0 37.5 41.0 34.0 41.0 24-25 37.986900621225494 40.0 37.0 41.0 34.0 41.0 26-27 37.66714157442497 40.0 36.5 41.0 33.5 41.0 28-29 37.41875808737149 39.5 35.5 41.0 33.0 41.0 30-31 37.07703407607369 39.0 35.0 41.0 33.0 41.0 32-33 36.63275594547982 38.0 35.0 41.0 33.0 41.0 34-35 36.3674526678472 38.0 35.0 40.0 33.0 41.0 36-37 35.981841027031294 38.0 35.0 40.0 32.0 41.0 38-39 35.542771500150934 36.5 35.0 40.0 30.5 41.0 40-41 35.2145439058952 36.0 35.0 40.0 30.0 41.0 42-43 35.04549116421618 36.0 35.0 40.0 30.0 41.0 44-45 34.702089594136524 36.0 35.0 40.0 27.5 41.0 46-47 34.17431421973809 36.0 35.0 40.0 23.5 41.0 48-49 33.79344012028433 36.0 34.0 40.0 22.5 41.0 50-51 33.1227492352447 35.5 33.5 39.5 20.5 40.5 52-53 33.04406740229302 35.0 33.5 39.5 20.0 40.5 54-55 33.23404492588939 35.0 34.0 40.0 20.0 41.0 56-57 33.21978423421605 35.0 34.0 40.0 18.0 41.0 58-59 33.05426157611694 35.0 34.0 40.0 13.0 41.0 60-61 32.62741948198523 35.0 33.5 39.5 7.0 41.0 62-63 32.10400672177135 35.0 33.0 39.0 2.0 41.0 64-65 31.503819450452895 35.0 32.0 38.5 2.0 40.0 66-67 31.098622190689888 35.0 32.0 38.0 2.0 40.0 68-69 30.75763649752644 35.0 31.5 37.0 2.0 39.0 70-71 30.207821173195207 35.0 31.0 36.5 2.0 39.0 72-73 29.583816412581402 35.0 30.0 35.5 2.0 38.0 74-75 29.092289766795297 35.0 29.0 35.0 2.0 37.0 76-77 28.4051405400105 34.0 28.0 35.0 2.0 36.5 78-79 27.877526672703958 34.0 27.0 35.0 2.0 36.0 80-81 27.529610594865225 34.0 26.5 35.0 2.0 36.0 82-83 27.0851305804065 33.5 26.0 35.0 2.0 35.0 84-85 26.586911965175993 33.0 25.0 35.0 2.0 35.0 86-87 25.931731529068394 33.0 22.0 35.0 2.0 35.0 88-89 25.16893257271184 32.5 19.0 35.0 2.0 35.0 90-91 24.414758864143693 32.0 10.5 35.0 2.0 35.0 92-93 23.950723032641736 31.5 2.0 35.0 2.0 35.0 94-95 23.560571888921572 32.0 2.0 35.0 2.0 35.0 96-97 22.64442963962769 31.0 2.0 35.0 2.0 35.0 98-99 21.592812742140467 31.0 2.0 34.5 2.0 35.0 100-101 19.379970428820556 26.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.35973294800655964 1101 2 0.2693434465368867 1101 3 0.3785595443859684 1101 4 0.012896286846938665 1101 5 -3.1441416204813777E-4 1101 6 0.2917052740170334 1101 7 0.06282979791082255 1101 8 0.1161196913685103 1101 9 -0.04107592226915102 1101 10-11 0.16570870224889944 1101 12-13 0.13872531736191007 1101 14-15 0.3992976652946254 1101 16-17 0.39766653882124814 1101 18-19 0.17869011275348612 1101 20-21 0.3760357562932697 1101 22-23 0.3794835903217191 1101 24-25 0.4976429069259254 1101 26-27 0.39787213450507153 1101 28-29 0.639148044435025 1101 30-31 0.7326055483818692 1101 32-33 0.820756704507879 1101 34-35 0.7546852620272944 1101 36-37 0.6576140514361839 1101 38-39 0.7912985109331174 1101 40-41 0.5744805945337461 1101 42-43 0.7704991351397297 1101 44-45 0.6249402994661466 1101 46-47 0.36614422940687774 1101 48-49 0.6221308896287354 1101 50-51 0.8260493824722062 1101 52-53 0.5356480278499163 1101 54-55 0.5631121969292892 1101 56-57 0.5720619687055759 1101 58-59 0.7304593924805971 1101 60-61 0.6499357118729634 1101 62-63 0.7000346245164479 1101 64-65 0.32555334770597355 1101 66-67 -0.25415911346775744 1101 68-69 0.4549312540346584 1101 70-71 0.5667349761186387 1101 72-73 0.5966319709018002 1101 74-75 0.40932657979310605 1101 76-77 0.2981360249164702 1101 78-79 0.23551476695222107 1101 80-81 0.4336918311387059 1101 82-83 0.22929719593060227 1101 84-85 0.2342053913500486 1101 86-87 0.3453924850629555 1101 88-89 0.5994621563488174 1101 90-91 0.735025631357832 1101 92-93 0.26794643422945086 1101 94-95 0.47007344769621895 1101 96-97 0.36012296602654104 1101 98-99 0.6757719981837305 1101 100-101 1.0724137697109697 1102 1 0.21136693493466652 1102 2 0.10272035285496628 1102 3 0.09010638534457271 1102 4 0.08274378140031757 1102 5 0.22957665337607835 1102 6 0.14486431541136824 1102 7 -0.12553621516107683 1102 8 0.026272196815135374 1102 9 0.03522037402714062 1102 10-11 0.16653658895913992 1102 12-13 0.07922640668653713 1102 14-15 -0.0018788052936073996 1102 16-17 0.10200205080381153 1102 18-19 0.2211519863918312 1102 20-21 0.23871549485536292 1102 22-23 0.3898975336768373 1102 24-25 0.375311752241835 1102 26-27 0.15763248308894617 1102 28-29 -0.1414401908590932 1102 30-31 -0.08896307906911716 1102 32-33 -0.05896007109124923 1102 34-35 -0.08690515191605641 1102 36-37 -0.0997715694788468 1102 38-39 -0.0013158681518490312 1102 40-41 -0.007044459932487257 1102 42-43 -0.03921764045939824 1102 44-45 -0.10776122558831958 1102 46-47 -0.12400827603973852 1102 48-49 -0.1767579992601469 1102 50-51 -0.3073057373535022 1102 52-53 -0.21670491332655217 1102 54-55 -0.036539219192711414 1102 56-57 -0.10841733412667054 1102 58-59 -0.2582769909616687 1102 60-61 -0.10773313344293456 1102 62-63 -0.21674097461209385 1102 64-65 -0.2586950183070975 1102 66-67 -0.2251395056246217 1102 68-69 -0.8173345411723112 1102 70-71 -0.6782105576504236 1102 72-73 -0.49776890055789735 1102 74-75 -0.9409130716230152 1102 76-77 -0.5138029729048803 1102 78-79 -0.40799285832011023 1102 80-81 -0.2583125261597701 1102 82-83 0.117510703555876 1102 84-85 -0.12176410756062594 1102 86-87 0.0021245112067482808 1102 88-89 -0.04398010944639097 1102 90-91 0.3079885943207934 1102 92-93 -0.029352040716084105 1102 94-95 0.6214895697005787 1102 96-97 0.44293342354288257 1102 98-99 0.7575367040660872 1102 100-101 0.5149627893188118 1103 1 0.3631145905186415 1103 2 0.20030963011176794 1103 3 0.24860785356471027 1103 4 0.16183348491457394 1103 5 0.11905756651427879 1103 6 -0.0027391704274108974 1103 7 0.2165496046741069 1103 8 0.20056413581295374 1103 9 0.23868253183712795 1103 10-11 0.2894526636015584 1103 12-13 0.17759005166143282 1103 14-15 0.5698532208501845 1103 16-17 0.07674866442511075 1103 18-19 0.5124823832848904 1103 20-21 0.5723401041193554 1103 22-23 0.4702565371816263 1103 24-25 0.45054145765056575 1103 26-27 0.47644701373211973 1103 28-29 0.514413744918123 1103 30-31 0.8925089300243769 1103 32-33 0.8321335161020826 1103 34-35 0.6993954069548352 1103 36-37 0.5101744379096118 1103 38-39 0.5724337766336021 1103 40-41 0.8185144109588691 1103 42-43 0.8465619370720958 1103 44-45 0.9452059999492377 1103 46-47 1.222472731822343 1103 48-49 0.8783144645079659 1103 50-51 0.8483682230519136 1103 52-53 1.1085224239851854 1103 54-55 1.3389817621466804 1103 56-57 1.1259508575944608 1103 58-59 1.0256767837849523 1103 60-61 1.2800806394091993 1103 62-63 1.0822810013280417 1103 64-65 1.2960364394934132 1103 66-67 1.3477007899138869 1103 68-69 1.2292790801216178 1103 70-71 1.3801890823988323 1103 72-73 1.2540474298389945 1103 74-75 1.1933603962182282 1103 76-77 1.0656964113899008 1103 78-79 1.233171771783141 1103 80-81 1.509344005051748 1103 82-83 1.2777029930320545 1103 84-85 1.4650266473886937 1103 86-87 1.433532581681316 1103 88-89 1.4968293061072693 1103 90-91 1.5441802207298103 1103 92-93 1.6238401540362126 1103 94-95 1.5285275539764136 1103 96-97 1.8305577486352398 1103 98-99 2.0077285199228605 1103 100-101 1.5781625619815003 1104 1 0.36176153260775834 1104 2 0.427397849487555 1104 3 0.3700763724173193 1104 4 0.4681567755514138 1104 5 0.15635686292416295 1104 6 0.4049371781203064 1104 7 0.33975003765263523 1104 8 0.33334421880050513 1104 9 0.5012569500222241 1104 10-11 0.5378064431433174 1104 12-13 0.3545113938948745 1104 14-15 0.5799154615140836 1104 16-17 0.581917345644662 1104 18-19 0.6874914036709612 1104 20-21 0.6323843040111115 1104 22-23 0.48885657326317045 1104 24-25 0.8212252029148601 1104 26-27 0.8372128445096791 1104 28-29 0.8449387313875363 1104 30-31 0.83940591721543 1104 32-33 0.8347539382561493 1104 34-35 1.0439245428018467 1104 36-37 1.0542300034878167 1104 38-39 1.0033962518275388 1104 40-41 1.191944863782247 1104 42-43 1.2357780655223962 1104 44-45 1.1669135590327713 1104 46-47 1.3402428923852128 1104 48-49 1.582923881791018 1104 50-51 1.650226422582854 1104 52-53 1.5963764741385305 1104 54-55 1.516390205228042 1104 56-57 1.7113243015778679 1104 58-59 1.457969765924581 1104 60-61 1.572909432481545 1104 62-63 1.629011111376748 1104 64-65 1.7237921674385746 1104 66-67 1.6662512138720302 1104 68-69 1.726591005072006 1104 70-71 1.6980719977876078 1104 72-73 1.6510210903116658 1104 74-75 1.3798613884606965 1104 76-77 0.9656648400386487 1104 78-79 0.8026332547346087 1104 80-81 1.271368179686423 1104 82-83 1.3578310825142843 1104 84-85 1.3154686463062504 1104 86-87 1.2900858921630025 1104 88-89 1.3766912942003593 1104 90-91 0.9187923441286934 1104 92-93 0.7118791221040439 1104 94-95 0.46269677641913276 1104 96-97 0.5551022191385755 1104 98-99 1.1694234504658887 1104 100-101 0.8596797909189 1105 1 -0.017979598199133306 1105 2 0.038797675717141544 1105 3 -0.06966833123917127 1105 4 0.1469819576449396 1105 5 0.14422135448330664 1105 6 -0.09129102952721269 1105 7 -0.014591380606248094 1105 8 -0.044054259490373227 1105 9 0.060298289516019565 1105 10-11 -0.02788120860128629 1105 12-13 -0.1764323256039475 1105 14-15 -0.03547946016742998 1105 16-17 -0.0869627284107608 1105 18-19 0.05211694467347172 1105 20-21 0.12286550189013923 1105 22-23 0.0027886566404831115 1105 24-25 0.14829522264673756 1105 26-27 0.28002695076947504 1105 28-29 0.4760278009038288 1105 30-31 0.3901180510998472 1105 32-33 0.38322898108974357 1105 34-35 0.2972619091244866 1105 36-37 0.21218243586758234 1105 38-39 0.16520370771502968 1105 40-41 0.16980131642981178 1105 42-43 0.2579398831284223 1105 44-45 0.23661457648289286 1105 46-47 -0.20947655772075535 1105 48-49 -0.5753229046743726 1105 50-51 -0.6454248510568235 1105 52-53 -0.05439980863627625 1105 54-55 -0.3096079596264829 1105 56-57 -0.11998847704350624 1105 58-59 -0.22564628266616893 1105 60-61 -0.32298666255625363 1105 62-63 -0.5130116898997308 1105 64-65 -0.433496445727382 1105 66-67 -0.45539416456582416 1105 68-69 -0.4915071021297237 1105 70-71 -0.4924583267732956 1105 72-73 -0.48123930546262983 1105 74-75 -0.771986749191452 1105 76-77 -0.7669824791061401 1105 78-79 -0.595550682058434 1105 80-81 -0.4503981884307642 1105 82-83 -0.4533316364242559 1105 84-85 -0.7332418797958518 1105 86-87 -0.7429223659916211 1105 88-89 -0.7471871152319629 1105 90-91 -0.7581489718167731 1105 92-93 -0.7654434331330222 1105 94-95 -0.8563018512818203 1105 96-97 -0.9064328069675867 1105 98-99 -0.693366945064799 1105 100-101 -0.8847660519894021 1106 1 -0.02339621597366559 1106 2 0.04020955442427265 1106 3 0.1535864087745331 1106 4 0.09843679834489905 1106 5 0.15095143582720283 1106 6 0.11573278315092494 1106 7 0.17011112178789034 1106 8 -0.09150549668220975 1106 9 -0.02015608560463278 1106 10-11 -0.5522238144070215 1106 12-13 -0.16479284296475072 1106 14-15 -0.01771931299894902 1106 16-17 -0.043569223052820405 1106 18-19 0.031006897623463203 1106 20-21 -0.1285870676912637 1106 22-23 -0.24747536089255107 1106 24-25 0.020628077783435117 1106 26-27 -0.15963699938703968 1106 28-29 -0.16623128073153026 1106 30-31 0.022590504324270455 1106 32-33 0.09554071482380522 1106 34-35 -0.2232708952905682 1106 36-37 -0.3292503371303326 1106 38-39 -0.09098603667057859 1106 40-41 0.00922377097904814 1106 42-43 -0.270167101696714 1106 44-45 -0.18094192705437706 1106 46-47 -0.2330734795980618 1106 48-49 -0.016538785849448345 1106 50-51 0.0261203044580256 1106 52-53 -0.028957602925927972 1106 54-55 -0.09959573343821404 1106 56-57 0.01332996784591245 1106 58-59 0.3063007851305031 1106 60-61 0.0013025719632295818 1106 62-63 0.009383431737596482 1106 64-65 -0.2301934822961762 1106 66-67 -0.4071915656491498 1106 68-69 -0.5868675854237537 1106 70-71 -0.2689215895379249 1106 72-73 0.1539906642179396 1106 74-75 -0.04068846426448758 1106 76-77 -0.10946337332122624 1106 78-79 0.03547393308159741 1106 80-81 -0.1382424048380848 1106 82-83 0.18410699605955116 1106 84-85 -0.2637808540830058 1106 86-87 -0.15654604921600068 1106 88-89 -0.33741297797312697 1106 90-91 -0.4858361782273839 1106 92-93 -0.3036890697464827 1106 94-95 -0.5414245106102484 1106 96-97 -0.360128484650442 1106 98-99 -0.2934929921450866 1106 100-101 -0.4160431398097728 1107 1 0.3985902113420039 1107 2 0.2689302059718415 1107 3 0.22279315180742643 1107 4 0.30651214179106745 1107 5 0.39269423015588956 1107 6 0.4047560857395709 1107 7 0.39902629801623846 1107 8 0.38462485687557546 1107 9 0.31471998749260166 1107 10-11 0.2629593874692233 1107 12-13 0.379634024930958 1107 14-15 0.5005458204706699 1107 16-17 0.41618435454968505 1107 18-19 0.6230776312017241 1107 20-21 0.624441828399533 1107 22-23 0.5694603982491984 1107 24-25 0.6906536596140995 1107 26-27 0.5504569964068153 1107 28-29 0.6462469269988063 1107 30-31 0.7837967775617045 1107 32-33 0.6647731497956713 1107 34-35 0.6284297275554422 1107 36-37 0.6252969525536187 1107 38-39 0.7461013783166379 1107 40-41 0.94832837020315 1107 42-43 1.0711906805751141 1107 44-45 0.8359101497718626 1107 46-47 1.1282230824534807 1107 48-49 1.1081882372989895 1107 50-51 1.0067008688732173 1107 52-53 1.2626330584212866 1107 54-55 1.0996966371569883 1107 56-57 1.0225827361523372 1107 58-59 1.1906301704692126 1107 60-61 1.039923061651585 1107 62-63 0.924449551777677 1107 64-65 0.7906408450705129 1107 66-67 0.9910738614426293 1107 68-69 0.8991248024217562 1107 70-71 1.2558325936602799 1107 72-73 1.2305324558269533 1107 74-75 0.9387341277585293 1107 76-77 0.9743198748005106 1107 78-79 1.213695243443894 1107 80-81 1.0316235199432597 1107 82-83 1.0042560827111195 1107 84-85 1.0164044214997432 1107 86-87 1.041867290038713 1107 88-89 0.9513111970403614 1107 90-91 1.2113549587877301 1107 92-93 1.446647678167885 1107 94-95 0.9671217293744832 1107 96-97 1.1334246738064273 1107 98-99 1.2100983738953097 1107 100-101 0.8373325974571202 1108 1 0.28536149626285123 1108 2 0.26386088368206373 1108 3 0.10479636514833501 1108 4 0.08444535416242616 1108 5 0.15174560205854704 1108 6 0.13594696095945125 1108 7 0.09160514259857422 1108 8 0.2224619460319346 1108 9 -0.1002892231612833 1108 10-11 0.030923625201047855 1108 12-13 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2316 62-63 -0.6437252491012444 2316 64-65 -0.48743770021398447 2316 66-67 -0.5424582182597533 2316 68-69 -0.7787022372765584 2316 70-71 -0.7085309533179043 2316 72-73 -0.5635207410518639 2316 74-75 -0.7477876908756649 2316 76-77 -0.7466138434352914 2316 78-79 -0.530714827571213 2316 80-81 -0.33694008039367773 2316 82-83 -0.35201402049909447 2316 84-85 -0.19880672028765645 2316 86-87 -0.40561330745942215 2316 88-89 -0.3119333149518688 2316 90-91 -0.2215883760144699 2316 92-93 -0.5546233393355848 2316 94-95 -0.5430913758951448 2316 96-97 -0.2482972551424929 2316 98-99 -0.13132120876729303 2316 100-101 0.07106042584577565 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2289.0 3 395.0 4 333.0 5 329.0 6 368.0 7 416.0 8 570.0 9 737.0 10 920.0 11 1359.0 12 2185.0 13 3088.0 14 3694.0 15 4715.0 16 6366.0 17 9176.0 18 7824.0 19 5483.0 20 4790.0 21 4981.0 22 5258.0 23 5816.0 24 6303.0 25 6847.0 26 7803.0 27 9042.0 28 10333.0 29 12485.0 30 14806.0 31 17745.0 32 21787.0 33 28824.0 34 43042.0 35 97932.0 36 71444.0 37 61145.0 38 36185.0 39 3286.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.6928832437676 17.503869587649785 13.484522615590041 13.318724552992578 2 13.640202134064822 26.15893339078547 40.15923732285857 20.041627152291127 3 23.942957506689776 35.96724269607155 29.49080744791977 10.598992349318904 4 10.460315444789465 18.807019974065653 38.07000734934798 32.6626572317969 5 9.468393790359201 45.53152924334434 30.508285421813785 14.491791544482671 6 20.4938494969695 39.164393870861566 19.262016625894283 21.07974000627465 7 21.661042254279877 44.51571692980195 18.341420430277168 15.48182038564101 8 23.175590022564073 30.446876328500554 20.070289100957385 26.307244547977987 9 29.123585909201953 22.294977272596118 20.651063657890536 27.930373160311394 10-11 22.700068786046536 29.0893785685768 23.50606808375543 24.704484561621236 12-13 33.89806368541649 28.962150395664104 22.95178989528831 14.187996023631102 14-15 28.741543874701698 28.44759342497482 30.511402603423715 12.299460096899766 16-17 15.21864178530204 33.65881108118507 32.85475182762601 18.26779530588688 18-19 13.998914969382515 34.69443113807015 37.486461164945815 13.820192727601524 20-21 17.285536173995357 31.553975272156354 34.15057201897879 17.009916534869493 22-23 15.681925086982327 31.27554519666134 32.604448531569034 20.438081184787304 24-25 17.010586032441253 32.265557195557754 35.37382107469109 15.350035697309906 26-27 19.78321451623919 28.863174845342183 37.0616024387405 14.29200819967813 28-29 15.059972661431745 30.04674106374157 40.61816716158903 14.275119113237658 30-31 13.912489706708534 37.11164469481834 36.92675024434543 12.049115354127705 32-33 13.186747642818593 36.117569984309505 34.73245834708216 15.963224025789744 34-35 14.65277403664853 32.2813170998253 35.28277165087697 17.78313721264921 36-37 17.748529629622503 32.45627367647427 36.71808714289288 13.077109551010357 38-39 20.27671131696583 29.07353835027275 37.94634270194579 12.70340763081563 40-41 14.021668566152604 32.402825665661624 40.98263533236195 12.592870435823825 42-43 12.695942703319258 36.44777121336127 37.005003938329324 13.851282144990146 44-45 13.301525671609932 37.2865987628858 33.60169845990533 15.810177105598939 46-47 13.154245733880291 34.34281659098908 31.558035190559437 20.944902484571195 48-49 15.592921426215495 33.501293840817674 33.43734704455217 17.468437688414667 50-51 18.998839370625404 34.41309366865618 33.46449310690002 13.123573853818392 52-53 13.55447174484976 34.529766758465776 37.177004323276456 14.738757173408004 54-55 11.350008269517021 33.947905889050006 37.82044378459254 16.88164205684043 56-57 14.777872759424096 37.43263007383888 31.574559577371204 16.214937589365817 58-59 13.188762681502578 33.87618641941002 33.74330167625161 19.191749222835792 60-61 14.260616201246249 31.19624586506654 35.42301330871606 19.12012462497115 62-63 20.407664845229085 30.401693811951873 33.51624630656496 15.674395036254078 64-65 17.82513043294314 31.470541289502478 37.00093654026258 13.703391737291803 66-67 13.822979103088482 32.55501135513597 37.93846112428345 15.683548417492101 68-69 13.064225970187426 36.826979195812434 35.86710396132423 14.24169087267591 70-71 13.910859094895903 35.32850800929417 32.3694546601628 18.39117823564713 72-73 13.703310832838861 33.40039564197575 31.489793033277586 21.406500491907803 74-75 15.528567320942766 33.419914085992865 33.99325112489265 17.058267468171717 76-77 18.05077582998953 30.187993457585367 35.97193415661602 15.789296555809084 78-79 14.7125165855816 30.461031094359942 38.745937734361476 16.080514585696978 80-81 13.727243532422978 36.4051559852851 35.30278817807879 14.564812304213131 82-83 13.723929111606623 34.89092798949231 32.93823755938696 18.446905339514096 84-85 15.026441799196169 31.328724447606778 33.31916693909733 20.32566681409973 86-87 17.60148750922963 31.23980894659119 34.89282088358356 16.26588266059562 88-89 19.89230351980334 27.9410718098356 36.22335238234463 15.943272288016436 90-91 15.712638227760683 30.29935201153433 38.422532339308574 15.565477421396412 92-93 14.233138981929455 34.24835243670116 35.48177703152993 16.036731549839452 94-95 14.343026096591629 35.047168540968535 32.98181356499525 17.62799179744459 96-97 15.145878696016805 31.76237391418086 30.690019312000373 22.401728077801973 98-99 17.391220724368132 30.905277451344322 32.098228796624156 19.60527302766339 100-101 20.489742466333816 30.952089782909088 32.84943259782091 15.708735152936192 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13534.0 1 8202.0 2 4710.0 3 6001.0 4 4044.5 5 3517.0 6 7771.5 7 15971.0 8 21526.5 9 20059.5 10 13178.0 11 7425.0 12 6180.0 13 5775.5 14 4862.0 15 3517.0 16 2449.5 17 1916.0 18 1746.0 19 1589.0 20 1436.0 21 1436.0 22 1534.0 23 1627.0 24 1642.0 25 1743.5 26 2037.5 27 2296.5 28 2437.5 29 2788.0 30 3372.5 31 3950.0 32 4915.0 33 6144.5 34 7135.0 35 7964.0 36 9646.0 37 11160.5 38 11014.0 39 10561.0 40 9591.0 41 8092.5 42 7104.5 43 6869.5 44 8056.0 45 8671.0 46 10294.0 47 18289.0 48 65905.0 49 69493.0 50 26864.5 51 15829.5 52 6824.5 53 3577.0 54 2451.0 55 1814.0 56 1418.5 57 1129.0 58 946.0 59 868.0 60 786.5 61 698.0 62 588.0 63 402.5 64 229.5 65 178.0 66 175.5 67 143.0 68 107.5 69 103.5 70 108.5 71 87.5 72 68.0 73 74.0 74 65.0 75 41.0 76 30.0 77 25.5 78 17.0 79 11.0 80 8.5 81 5.5 82 5.0 83 2.5 84 0.5 85 1.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.5006719848644783 2 0.047683046177569355 3 0.053451156602275327 4 0.06287240362929508 5 0.07556224656364821 6 0.10574869111960945 7 0.06594872918913826 8 0.047683046177569355 9 0.046913964787608564 10-11 0.07181297478758933 12-13 0.10219168969104078 14-15 0.05489318420845182 16-17 0.05787337459454991 18-19 0.0576811042470597 20-21 0.06979413613894224 22-23 0.05989221324319699 24-25 0.08729073776055035 26-27 0.06239172776056958 28-29 0.06200718706558918 30-31 0.06633326988411867 32-33 0.06912118992272655 34-35 0.06787143266404025 36-37 0.06421829606172647 38-39 0.04018450262545159 40-41 0.04297242266405948 42-43 0.040472908146686894 44-45 0.019323169922765 46-47 0.02537968586870627 48-49 0.026437172779902363 50-51 0.023937658262529776 52-53 0.024033793436274875 54-55 0.022880171351333686 56-57 0.022591765830098386 58-59 0.019515440270255202 60-61 0.027110118996118062 62-63 0.01740046644786301 64-65 0.0196115754440003 66-67 0.01384346501929433 68-69 0.016439114710412015 70-71 0.0394154212354908 72-73 0.03585841980692212 74-75 0.031243931467157336 76-77 0.021053603050176794 78-79 0.01365119467180413 80-81 0.016150709189176715 82-83 0.019323169922765 84-85 0.01749660162160811 86-87 0.007114002857137363 88-89 0.04114585436290259 90-91 0.050278695868687044 92-93 0.03201301285711814 94-95 0.05056710138992234 96-97 0.041722665405373185 98-99 0.03307049976831423 100-101 0.16314138984543386 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 520101.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.320888960897868 #Duplication Level Percentage of deduplicated Percentage of total 1 74.70633127549054 14.433927312498044 2 10.713039299455502 4.139708854770297 3 4.259686741620764 2.469028036291909 4 2.3487609989979092 1.815206018293046 5 1.4818479267460158 1.4315309624798245 6 0.9699004389885681 1.1243603210894533 7 0.7119269195804596 0.9628542673041689 8 0.554943034209525 0.8577594194868784 9 0.4729514507763993 0.8224058217911728 >10 3.155676064799745 12.024666564657437 >50 0.3419648967558659 4.555591187140514 >100 0.20997844537640886 8.228887944372595 >500 0.027997126050187855 3.797903699927711 >1k 0.03199671548592897 13.747140209733761 >5k 0.005999384153611682 7.277238642483185 >10k+ 0.006999281512546964 22.311790737680006 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22253 4.278592042699398 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20724 3.9846106813868847 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 17500 3.364731081078483 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16178 3.11054968169644 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13389 2.5743076825462747 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13157 2.5297009619285484 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12291 2.363194841002036 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 7848 1.508937687103082 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 6832 1.3135910140530398 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6610 1.2709069969102154 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5548 1.066715887875624 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5537 1.064600914053232 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5294 1.0178792196131137 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4472 0.8598329939761701 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4328 0.8321460639375814 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4301 0.826954764555346 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4078 0.7840784770650316 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3425 0.6585259401539316 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3419 0.6573723180689904 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3238 0.6225713851732645 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3225 0.6200718706558919 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3197 0.6146883009261662 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 2916 0.5606603332814203 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2844 0.5468168682621259 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2795 0.5373956212351063 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2754 0.529512536988008 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2046 0.3933851309649472 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1949 0.37473490725839786 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1805 0.34704797721980923 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1684 0.32378326517349515 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1608 0.30917071876424 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1605 0.3085939077217694 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1430 0.2749465969109846 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1378 0.2649485388414943 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1310 0.2518741552121607 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1272 0.24456788200753315 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1208 0.2322625797681604 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1196 0.22995533559827802 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1163 0.22361041413110147 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1147 0.22053408857125828 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1107 0.21284327467165032 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1066 0.20496019042455216 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1065 0.20476792007706196 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1064 0.20457564972957176 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1064 0.20457564972957176 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 971 0.18669450741298327 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 946 0.18188774872572827 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 918 0.1765041789960027 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 895 0.17208196100372813 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 850 0.16342979536666916 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 832 0.15996892911184557 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 823 0.1582384959844338 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 799 0.15362400764466902 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 795 0.15285492625470823 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 745 0.14324140888019826 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 739 0.14208778679525708 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 726 0.13958827227788448 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 704 0.13535832463310013 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 701 0.1347815135906295 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 696 0.13382016185317852 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 685 0.13170518803078635 No Hit GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA 666 0.12805205142847256 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 663 0.12747524038600194 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 634 0.12189940030878617 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 589 0.11324723467172722 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 584 0.11228588293427623 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 574 0.11036317945937425 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 550 0.10574869111960945 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 527 0.10132647312733488 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 524 0.10074966208486427 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.9227034749019902E-4 0.0 8 0.0 0.0 0.0 1.9227034749019902E-4 0.0 9 0.0 0.0 0.0 1.9227034749019902E-4 0.0 10-11 0.0 0.0 0.0 4.8067586872549753E-4 0.0 12-13 0.0 0.0 0.0 6.729462162156966E-4 0.0 14-15 0.0 0.0 0.0 0.001153622084941194 0.0 16-17 0.0 0.0 0.0 0.0015381627799215922 0.0 18-19 0.0 0.0 0.0 0.002114973822392189 0.0 20-21 0.0 0.0 0.0 0.003557001428568682 0.0 22-23 0.0 0.0 0.0 0.0076908138996079605 0.0 24-25 0.0 0.0 0.0 0.016823655405392414 0.0 26-27 0.0 0.0 0.0 0.03085939077217694 0.0 28-29 0.0 0.0 0.0 0.05729656355207931 0.0 30-31 0.0 0.0 0.0 0.12180326513504108 0.0 32-33 0.0 0.0 0.0 0.20938240841682673 0.0 34-35 0.0 0.0 0.0 0.3518547359070642 0.0 36-37 0.0 0.0 0.0 0.5434521371810475 0.0 38-39 0.0 0.0 0.0 0.7488930034743252 0.0 40-41 0.0 0.0 0.0 0.96125560227725 0.0 42-43 0.0 0.0 0.0 1.147853974516488 0.0 44-45 0.0 0.0 0.0 1.3336832653657655 0.0 46-47 0.0 0.0 0.0 1.580366121195691 0.0 48-49 0.0 0.0 0.0 1.876174050809362 0.0 50-51 0.0 0.0 0.0 2.112474307874817 0.0 52-53 0.0 0.0 0.0 2.3074364402298784 0.0 54-55 0.0 0.0 0.0 2.486824674438234 0.0 56-57 0.0 0.0 0.0 2.678902751580943 0.0 58-59 0.0 0.0 0.0 2.9945145269861047 0.0 60-61 0.0 0.0 0.0 3.4575015237425037 0.0 62-63 0.0 0.0 0.0 3.8010886347074893 0.0 64-65 0.0 0.0 0.0 4.0048952030471 0.0 66-67 0.0 0.0 0.0 4.240618649070084 0.0 68-69 0.0 0.0 0.0 4.487205369726265 0.0 70-71 0.0 0.0 0.0 4.814737906675819 0.0 72-73 0.0 0.0 0.0 5.189857354629197 0.0 74-75 0.0 0.0 0.0 5.526907273779516 0.0 76-77 0.0 0.0 0.0 5.787049053933755 0.0 78-79 0.0 0.0 0.0 6.073628006867897 0.0 80-81 0.0 0.0 0.0 6.370301153045274 0.0 82-83 0.0 0.0 0.0 6.6773568979871225 0.0 84-85 0.0 0.0 0.0 7.242823989955797 0.0 86-87 0.0 0.0 0.0 7.841073176171552 0.0 88-89 0.0 0.0 0.0 8.19773467076587 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 25 5.1101335E-5 76.48138 1 GGAGCTC 25 0.005007485 57.035183 8 GGTAATC 25 0.005007485 57.035183 8 GCTGTGC 25 0.005007485 57.035183 8 GTCTATG 25 0.0050113034 57.024204 2 TGGCCGT 20 5.3971226E-4 47.4836 90-91 GGGAACC 85 3.6152778E-7 39.14556 7 GTACCCA 50 0.0015656645 38.24069 1 GCGTATC 50 0.0015656645 38.24069 1 TACCTGG 170 0.0 36.33895 2 GATGGAA 80 1.0719292E-5 35.646988 9 AGTACTC 230 0.0 35.123604 5 GGACCCG 70 2.0443642E-4 33.949512 9 ATGGGTT 185 0.0 33.39255 5 TGGGGAC 100 1.2930795E-6 33.26412 6 GTCACTA 60 0.0038287614 31.86724 1 GGCTTCG 75 3.0632984E-4 31.689262 7 ACTCAAT 60 0.0039370656 31.686213 8 AGTACCC 60 0.003940779 31.680109 5 AAAGTAC 215 0.0 30.943367 3 >>END_MODULE