##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938045_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 520101 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.39614613315491 33.0 31.0 34.0 31.0 34.0 2 32.5424215681185 34.0 31.0 34.0 31.0 34.0 3 32.56018927093007 34.0 31.0 34.0 31.0 34.0 4 35.99156894526256 37.0 35.0 37.0 35.0 37.0 5 36.02948465778762 37.0 35.0 37.0 35.0 37.0 6 36.16944016642921 37.0 36.0 37.0 35.0 37.0 7 36.15436040307556 37.0 36.0 37.0 35.0 37.0 8 36.20955737443304 37.0 37.0 37.0 35.0 37.0 9 37.8097215733098 39.0 38.0 39.0 35.0 39.0 10-11 37.8082199418959 39.0 38.0 39.0 35.0 39.0 12-13 37.73265769533225 39.0 37.5 39.0 35.0 39.0 14-15 38.60463832986285 40.0 38.0 41.0 34.0 41.0 16-17 38.60370101191884 40.0 38.0 41.0 34.5 41.0 18-19 38.87614232620203 40.0 38.0 41.0 35.0 41.0 20-21 38.938385044443294 40.0 38.0 41.0 35.0 41.0 22-23 38.77037152399245 40.0 38.0 41.0 35.0 41.0 24-25 38.64022564847981 40.0 38.0 41.0 35.0 41.0 26-27 38.244731311802894 40.0 36.5 41.0 34.5 41.0 28-29 37.97720635030504 40.0 36.0 41.0 34.0 41.0 30-31 37.633685572609934 39.0 35.5 41.0 34.0 41.0 32-33 37.21171849313883 38.0 35.0 41.0 33.0 41.0 34-35 36.953920488520495 38.0 35.0 41.0 33.0 41.0 36-37 36.63034295261882 38.0 35.0 41.0 33.0 41.0 38-39 36.29519074179822 38.0 35.0 40.5 33.0 41.0 40-41 36.049983560885295 37.5 35.0 40.0 32.5 41.0 42-43 35.96555188319192 38.0 35.0 40.5 32.5 41.0 44-45 35.720185117890566 38.0 35.0 41.0 31.0 41.0 46-47 35.154977590891 38.0 35.0 41.0 29.0 41.0 48-49 34.72698572008129 37.0 35.0 40.0 25.5 41.0 50-51 34.48040957429423 37.0 35.0 40.0 24.5 41.0 52-53 33.8503617566588 35.5 34.0 40.0 23.0 41.0 54-55 33.78095696797352 36.0 34.0 40.0 23.0 41.0 56-57 33.79156836845151 36.0 35.0 40.0 22.5 41.0 58-59 33.611614859421536 36.0 34.0 40.0 22.0 41.0 60-61 33.21492267847975 35.0 33.5 39.5 20.5 41.0 62-63 32.706708889234974 35.0 33.0 39.0 16.0 41.0 64-65 32.131897458378276 35.0 33.0 39.0 10.0 40.0 66-67 31.722982651446543 35.0 32.5 38.0 4.5 40.0 68-69 31.429271429972257 35.0 33.0 37.0 2.0 39.0 70-71 30.90919456028733 35.0 31.5 37.0 2.0 39.0 72-73 30.40357834343714 35.0 31.0 36.0 2.0 38.5 74-75 30.0107479124247 35.0 31.0 35.0 2.0 37.0 76-77 28.522193766210794 33.0 28.5 35.0 2.0 36.0 78-79 29.157330018592543 34.0 29.5 35.0 2.0 36.0 80-81 29.17138786504929 34.0 30.5 35.0 2.0 36.0 82-83 28.950391366292315 34.0 30.0 35.0 2.0 36.0 84-85 28.6500977694717 34.0 30.0 35.0 2.0 35.0 86-87 28.164168113501034 34.0 29.0 35.0 2.0 35.0 88-89 27.53433275459959 33.5 27.5 35.0 2.0 35.0 90-91 27.09854912795784 33.0 25.5 35.0 2.0 35.0 92-93 26.970241356967204 33.0 26.0 35.0 2.0 35.0 94-95 26.83042716703102 34.0 25.5 35.0 2.0 35.0 96-97 26.232279884099434 33.0 24.5 35.0 2.0 35.0 98-99 25.63448061049681 33.0 21.5 35.0 2.0 35.0 100-101 23.68532746524233 31.0 8.5 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.014499645624802326 1101 2 0.23418115714835608 1101 3 0.2902128980228724 1101 4 0.05920212363248112 1101 5 0.20597016357145748 1101 6 0.08858247594794477 1101 7 0.18216042587164338 1101 8 0.0927846753537267 1101 9 -0.09656368332404242 1101 10-11 0.19714345431219016 1101 12-13 0.28276341256676574 1101 14-15 0.2905865563785923 1101 16-17 0.1040868044123755 1101 18-19 0.1506197808138907 1101 20-21 0.08887659374783397 1101 22-23 0.28279694877642925 1101 24-25 0.16530359476330148 1101 26-27 0.10551391627876683 1101 28-29 0.1927145842282485 1101 30-31 0.201708974884653 1101 32-33 0.2065746664240251 1101 34-35 0.23710829875831507 1101 36-37 0.2566879776513815 1101 38-39 0.4990493177850652 1101 40-41 0.21307984636004562 1101 42-43 0.167807601869562 1101 44-45 -0.13837876776624114 1101 46-47 -0.3814636093305168 1101 48-49 -0.01775530510419543 1101 50-51 -0.17756222827152612 1101 52-53 -0.03773840729400746 1101 54-55 0.07276376831227793 1101 56-57 0.22440100043554878 1101 58-59 0.22115334058493374 1101 60-61 0.10112344766699977 1101 62-63 -0.17170732638096098 1101 64-65 -0.05762620385038986 1101 66-67 -0.08192657163587214 1101 68-69 -0.16499981139896036 1101 70-71 -0.25691150229110704 1101 72-73 -0.40491488102437856 1101 74-75 -0.15204009229767834 1101 76-77 -0.37242522994202076 1101 78-79 -0.15801527260233428 1101 80-81 -0.11951415429374279 1101 82-83 -0.028876871120900205 1101 84-85 -0.14175973515043339 1101 86-87 -0.10031745117609958 1101 88-89 -0.30627639143795804 1101 90-91 -0.21298500807566967 1101 92-93 -0.19337309947659875 1101 94-95 -0.3270206999537244 1101 96-97 -0.1835512053207431 1101 98-99 -0.11965614155592874 1101 100-101 0.13042391278148457 1102 1 -0.013671758914561849 1102 2 -0.0865595835923827 1102 3 -0.1585016989270187 1102 4 0.04460517374141659 1102 5 0.028628115641176066 1102 6 -0.09743059594747905 1102 7 -0.06433194885602234 1102 8 -0.10290159915607688 1102 9 -0.024014663716201312 1102 10-11 -0.12586308163552218 1102 12-13 -0.1591973717469628 1102 14-15 -0.17416289895910353 1102 16-17 0.05816087848645424 1102 18-19 0.015608887567701402 1102 20-21 -0.12487068294061743 1102 22-23 -0.2546322451233536 1102 24-25 -0.15238703704497425 1102 26-27 -0.3580372819783122 1102 28-29 -0.11902833516172251 1102 30-31 -0.1658073649846301 1102 32-33 -0.2127499523123575 1102 34-35 0.2094394534424069 1102 36-37 -0.001983046313753789 1102 38-39 -0.024937610319511805 1102 40-41 0.09769858274348309 1102 42-43 -0.18593967481888996 1102 44-45 -0.37681014031525706 1102 46-47 -0.3276945461496936 1102 48-49 -0.4918729521630212 1102 50-51 -0.28215917816259406 1102 52-53 -0.311485683982454 1102 54-55 -0.12638655848510183 1102 56-57 -0.16302819346422837 1102 58-59 -0.5274305374107087 1102 60-61 -0.393255637300328 1102 62-63 -0.4255853220236645 1102 64-65 -0.5539442866390587 1102 66-67 -0.5622097960367434 1102 68-69 -0.4975488310067995 1102 70-71 -0.7929463606789078 1102 72-73 -1.00783427100259 1102 74-75 -0.7848505498140206 1102 76-77 -0.570268367196924 1102 78-79 -0.8858801963496106 1102 80-81 -0.6321067468863362 1102 82-83 -0.8363060650206791 1102 84-85 -0.8300821752375818 1102 86-87 -1.1773109152283894 1102 88-89 -0.9342938206318543 1102 90-91 -1.2096952477270868 1102 92-93 -1.0259656920691889 1102 94-95 -1.3680882380800838 1102 96-97 -1.175141619264096 1102 98-99 -0.781813004301025 1102 100-101 -0.5390532114015194 1103 1 0.1984955234573249 1103 2 0.208045891447874 1103 3 0.1014206274914713 1103 4 -0.005532175884425783 1103 5 0.1219121925569624 1103 6 -0.036731533713890485 1103 7 0.13761936306971023 1103 8 0.03162525506387226 1103 9 0.07203535049237786 1103 10-11 0.16173282629286945 1103 12-13 0.13298080387111355 1103 14-15 0.19766385106458273 1103 16-17 0.25558438895343727 1103 18-19 0.22774538467862016 1103 20-21 0.23631620727440605 1103 22-23 0.2072413932208761 1103 24-25 0.18023113099518184 1103 26-27 0.0682675394671719 1103 28-29 0.13996096103984712 1103 30-31 0.2135688782662939 1103 32-33 0.2839418161824767 1103 34-35 0.2558281055215943 1103 36-37 0.2411565766851993 1103 38-39 0.1961266124710619 1103 40-41 0.3527899912875796 1103 42-43 0.45036798834299674 1103 44-45 0.4343507008327947 1103 46-47 0.7062900138578883 1103 48-49 0.7423413132532914 1103 50-51 0.6530899456415185 1103 52-53 0.9088799501939206 1103 54-55 0.7687541064765355 1103 56-57 0.8104396477785514 1103 58-59 1.021153340584938 1103 60-61 1.0102538824496037 1103 62-63 0.9051525770006847 1103 64-65 0.8000791101592739 1103 66-67 0.5601024138713733 1103 68-69 0.9328504301469316 1103 70-71 1.0349000919117906 1103 72-73 0.8389498532751389 1103 74-75 0.41556860335449386 1103 76-77 0.7448211468695725 1103 78-79 0.5697866597648158 1103 80-81 0.7154375365275136 1103 82-83 0.8099878631786197 1103 84-85 0.5146653856225107 1103 86-87 0.7214458338480547 1103 88-89 0.3685786810258129 1103 90-91 0.4371599194605622 1103 92-93 0.18377665897751072 1103 94-95 0.592665290384442 1103 96-97 0.732173432360419 1103 98-99 0.8737496555455238 1103 100-101 1.0690954103660282 1104 1 0.09365157613638075 1104 2 0.02739461957344247 1104 3 0.04477260295601582 1104 4 -0.02687440933201657 1104 5 -0.05447243670516855 1104 6 -0.04930595189898668 1104 7 -7.533592828039559E-4 1104 8 -0.07946981980533252 1104 9 0.03451054443068102 1104 10-11 -0.10041763374682233 1104 12-13 0.16496258269124553 1104 14-15 0.3443578795689959 1104 16-17 0.22567278873695074 1104 18-19 0.13574195693191626 1104 20-21 0.10408636783727587 1104 22-23 0.2642953351295958 1104 24-25 0.2471984771975997 1104 26-27 0.3842967655180445 1104 28-29 0.14779065615079645 1104 30-31 0.23108195782609897 1104 32-33 0.3588245511635364 1104 34-35 0.4247754264669368 1104 36-37 0.3495049439953206 1104 38-39 0.5214513463696733 1104 40-41 0.26443530970720985 1104 42-43 0.2355023920954764 1104 44-45 0.27792367575612786 1104 46-47 0.3747051320249497 1104 48-49 0.21868084058505843 1104 50-51 0.29135893355419995 1104 52-53 0.46023300810480094 1104 54-55 0.6082363363159757 1104 56-57 0.5823862470929981 1104 58-59 0.8616097721202038 1104 60-61 0.8041109995342168 1104 62-63 0.641234121290772 1104 64-65 0.7636232198471689 1104 66-67 0.7221536496642678 1104 68-69 0.65001171464991 1104 70-71 0.3141396733658759 1104 72-73 0.2836097847202055 1104 74-75 0.09156986620854468 1104 76-77 0.1655139125199412 1104 78-79 0.10676573246912824 1104 80-81 0.07021383095291256 1104 82-83 0.1466602427564645 1104 84-85 0.2797294303636271 1104 86-87 0.26842851470679463 1104 88-89 0.411953207455543 1104 90-91 0.20882688680676154 1104 92-93 0.31028757364465775 1104 94-95 0.5131821585063996 1104 96-97 0.5818983105852062 1104 98-99 0.6763881184717349 1104 100-101 0.6159518058197513 1105 1 -0.025332435361697492 1105 2 0.005344453517132308 1105 3 0.035905439879797996 1105 4 -0.0068339711707352535 1105 5 -0.11549102364315189 1105 6 0.004177170446752143 1105 7 -0.2136951949154806 1105 8 -0.15988329594438255 1105 9 -0.15020577943189295 1105 10-11 -0.17812508232621127 1105 12-13 -0.1672170179616117 1105 14-15 -0.06927863170577808 1105 16-17 0.016811309739615865 1105 18-19 -0.00336369385451718 1105 20-21 -0.10903817033130991 1105 22-23 0.04187935799583187 1105 24-25 -0.4396148657715955 1105 26-27 -0.4721051313402782 1105 28-29 -0.3753415149237398 1105 30-31 -0.27113077288660037 1105 32-33 -0.2276871296185945 1105 34-35 -0.30794497369222285 1105 36-37 -0.3358386585738842 1105 38-39 -0.5173912127650482 1105 40-41 -0.34009693120029283 1105 42-43 -0.5264889843448302 1105 44-45 -0.18215785017545727 1105 46-47 -0.04117659041336452 1105 48-49 -0.3628999017556396 1105 50-51 -0.6634526392304281 1105 52-53 -0.8464250783094442 1105 54-55 -0.6062990714354868 1105 56-57 -0.8854337462485091 1105 58-59 -0.6945022367144773 1105 60-61 -0.7064695073232201 1105 62-63 -0.8409462913733492 1105 64-65 -0.9683480999147491 1105 66-67 -0.8465384437819914 1105 68-69 -0.8035168803269848 1105 70-71 -0.8025605564333098 1105 72-73 -0.9773523674344666 1105 74-75 -0.8258952782076605 1105 76-77 -0.7083482566869641 1105 78-79 -0.6851929199169717 1105 80-81 -0.8057676876705635 1105 82-83 -0.47358441623940095 1105 84-85 -0.5054996786164061 1105 86-87 -0.6386388253878899 1105 88-89 -0.9270330776740927 1105 90-91 -0.8167880087279862 1105 92-93 -0.8986916469869257 1105 94-95 -0.8598213087817292 1105 96-97 -0.826851922868034 1105 98-99 -0.7679992596250713 1105 100-101 -0.5548750652572174 1106 1 -0.08209904386249889 1106 2 0.15149471829170835 1106 3 0.16979166441014826 1106 4 0.10714968434599825 1106 5 0.12885862263013337 1106 6 0.04464093285746884 1106 7 0.15822318108333633 1106 8 -0.02325658834710964 1106 9 0.13964951246362034 1106 10-11 0.17889286329564413 1106 12-13 0.060945230748579604 1106 14-15 -0.23830663081246684 1106 16-17 -0.10381347497726523 1106 18-19 -0.026360661911233763 1106 20-21 0.09169842912286441 1106 22-23 0.0554038690429266 1106 24-25 0.03668979721333443 1106 26-27 0.2363778750150658 1106 28-29 0.3409995616621657 1106 30-31 0.1915585343086761 1106 32-33 0.28287167910401223 1106 34-35 0.24841283346710696 1106 36-37 0.34611200516052065 1106 38-39 0.31934375148375693 1106 40-41 0.39525908556056066 1106 42-43 0.27187164657190266 1106 44-45 0.3954746601297501 1106 46-47 0.33228236101120245 1106 48-49 0.44398120897274396 1106 50-51 0.39762152414626684 1106 52-53 0.17566154972297454 1106 54-55 -0.34040408038747927 1106 56-57 0.13508013217851556 1106 58-59 -0.05215420293537676 1106 60-61 -0.02065604943165056 1106 62-63 -0.3180666644424264 1106 64-65 -0.4343229586322508 1106 66-67 -0.34404778375708744 1106 68-69 -0.6018964372317548 1106 70-71 -0.5222628884935574 1106 72-73 -0.20377153264698578 1106 74-75 -0.19262036309071817 1106 76-77 -0.1803491499935994 1106 78-79 -0.2762250297482609 1106 80-81 -0.631175448082697 1106 82-83 -0.2954511242908957 1106 84-85 -0.21719063994304122 1106 86-87 -0.07609222014236039 1106 88-89 -0.09553493431352322 1106 90-91 0.0632174419565672 1106 92-93 0.3307575689093909 1106 94-95 0.14333176082427102 1106 96-97 0.37256742781916685 1106 98-99 0.4675327688244728 1106 100-101 0.4685004225601226 1107 1 0.11838249647934873 1107 2 0.11886595579900217 1107 3 0.11354833972643519 1107 4 -0.037550986213602755 1107 5 0.1537247492767051 1107 6 0.13153841101014052 1107 7 0.11410645159376287 1107 8 0.09254432114752831 1107 9 0.028062080960161495 1107 10-11 0.052505039806611364 1107 12-13 0.23042944672236132 1107 14-15 0.21623698859448837 1107 16-17 0.1376815756542058 1107 18-19 0.11832165726338673 1107 20-21 -0.035827180234882405 1107 22-23 0.044053537433399015 1107 24-25 0.1584218743332002 1107 26-27 0.2051786854122284 1107 28-29 0.03502324962989434 1107 30-31 0.18597420785955876 1107 32-33 0.3299796843451688 1107 34-35 0.37174882163412803 1107 36-37 0.2995490360532358 1107 38-39 0.22247963614897515 1107 40-41 0.18452829670793136 1107 42-43 0.24518689767391066 1107 44-45 0.38620904585383187 1107 46-47 0.16478560003064757 1107 48-49 0.24858667254707711 1107 50-51 0.34230494440399184 1107 52-53 0.061449870167514575 1107 54-55 0.06905936181850336 1107 56-57 0.08663798124938182 1107 58-59 0.266124877586833 1107 60-61 0.24294507954554945 1107 62-63 0.519351075474404 1107 64-65 0.27404784211208977 1107 66-67 0.5590917711851269 1107 68-69 0.6464528556893789 1107 70-71 0.8845432731674627 1107 72-73 0.6343050219905919 1107 74-75 0.7979029817061161 1107 76-77 0.7456709117404579 1107 78-79 0.7360538819412028 1107 80-81 0.8435767164631578 1107 82-83 0.7302481552337312 1107 84-85 0.8923157445037937 1107 86-87 0.7830517244511377 1107 88-89 0.6479677578346532 1107 90-91 0.5590516775663268 1107 92-93 0.7724038349530886 1107 94-95 0.523506392603732 1107 96-97 0.5626474031549087 1107 98-99 0.5995911702720313 1107 100-101 0.2984968623914348 1108 1 0.11977448251793987 1108 2 0.0706450663129985 1108 3 0.049929037520030306 1108 4 0.014271061183173117 1108 5 0.03966846040844274 1108 6 0.0029701482918227384 1108 7 0.0978700771287393 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72-73 0.27668596152433267 2316 74-75 0.41822847010358544 2316 76-77 0.5280960970721971 2316 78-79 0.5123428105993497 2316 80-81 0.4720708904666573 2316 82-83 0.1500541452034838 2316 84-85 0.49155253446515346 2316 86-87 0.3416309427154225 2316 88-89 0.3049084426852673 2316 90-91 0.49692020519447055 2316 92-93 -0.018417859876809217 2316 94-95 0.07932474501731335 2316 96-97 0.4125309431784707 2316 98-99 0.39154449035560646 2316 100-101 0.49209321241287185 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 7.0 4 16.0 5 37.0 6 72.0 7 189.0 8 288.0 9 416.0 10 684.0 11 1128.0 12 1658.0 13 2558.0 14 3211.0 15 4337.0 16 5627.0 17 8036.0 18 7874.0 19 5016.0 20 3702.0 21 3945.0 22 4151.0 23 4602.0 24 5118.0 25 5566.0 26 6044.0 27 6699.0 28 8010.0 29 9629.0 30 11447.0 31 13574.0 32 16803.0 33 22231.0 34 33895.0 35 89785.0 36 81054.0 37 87101.0 38 61113.0 39 4478.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.76743940119323 17.792505686395526 13.7852071040048 13.654847808406444 2 13.861860221835915 26.207251972985862 39.620479054812854 20.31040875036537 3 23.96787337016192 35.192004107163115 30.17111256818833 10.669009954486633 4 10.526224714045926 19.14820390654892 37.569049088542414 32.75652229086274 5 9.486042134123949 45.484434754018935 30.8818864028333 14.147636709023825 6 20.73212702917318 38.72305571417859 19.21511398747551 21.329703269172718 7 21.416609466238288 44.34292570096962 18.206271474194434 16.034193358597655 8 23.369826455783333 30.77727659018108 20.126206984068386 25.726689969967197 9 28.51561523627142 22.013801165542844 20.65541116052459 28.815172437661147 10-11 22.672711646391758 28.95303027681162 23.482554350020475 24.891703726776147 12-13 33.605203604684476 28.7856589393214 23.074172131951297 14.534965324042831 14-15 28.621556197738517 28.407078625113197 30.413035160478447 12.558330016669839 16-17 15.306176402445296 33.62657794022502 33.01256199522977 18.054683662099922 18-19 14.045733424854017 34.391973866614364 37.46426174916026 14.098030959371352 20-21 16.9259432302572 31.600304556230423 34.2824759037187 17.191276309793675 22-23 15.605334348520769 31.145873589937338 32.64558230036089 20.603209761181002 24-25 16.92743284557848 32.24537081546715 35.423404541696314 15.403791797258062 26-27 19.690760310393568 29.00006730963393 37.0235679875381 14.285604392434397 28-29 14.983820450258701 30.056469801057872 40.773907375682796 14.185802373000628 30-31 14.05669283466096 36.91859850298307 36.98262452869731 12.042084133658655 32-33 13.063152703392545 36.137236070613824 34.915443576336315 15.884167649657325 34-35 14.44284506855361 32.14322459179857 35.439124836897314 17.97480550275051 36-37 17.56919675046868 32.41484401288276 36.81257510935922 13.203384127289333 38-39 20.196304483484404 28.88029714898891 38.055339480027726 12.868058887498954 40-41 14.044884941593425 32.13241451986804 41.1802657020856 12.642434836452935 42-43 12.693298721276799 36.23584270743198 37.2694933179502 13.801365253341025 44-45 13.186010269544294 37.22858821165529 33.76816902735437 15.81723249144605 46-47 13.10620437184316 34.22940928781141 31.600112285882936 21.064274054462498 48-49 15.43382919855951 33.24594646039904 33.54435003970382 17.775874301337623 50-51 18.764912968827208 34.24354115835194 33.582131162985654 13.409414709835204 52-53 13.53544792261503 34.30151066812023 37.14393935024159 15.019102059023151 54-55 11.243969921226839 33.8116058227152 37.841784576457265 17.102639679600692 56-57 14.657537670567834 37.33457539977812 31.484942347736304 16.52294458191774 58-59 13.041216994391474 34.00906746958764 33.618758664182536 19.330956871838353 60-61 14.058230997440882 31.477059263489203 35.0690538953011 19.395655843768804 62-63 20.114266267513425 30.809112076308253 33.18095908294735 15.895662573230968 64-65 17.656955091414936 31.711917492948483 36.63336544248136 13.997761973155216 66-67 13.574574938329286 32.90130186252286 37.54520756545363 15.978915633694225 68-69 12.90629284040154 36.95209950413287 35.40797040563431 14.733637249831283 70-71 13.494975014468343 35.770648393292845 31.89034437541939 18.844032216819425 72-73 13.369230207209753 33.66298084410528 30.90707381835451 22.060715130330454 74-75 15.098605847710347 33.62058523248369 33.39745549422131 17.883353425584648 76-77 17.48939148357723 30.440337549822054 35.715755209084385 16.354515757516328 78-79 14.120622725201452 30.571562061984114 38.63220797498948 16.67560723782496 80-81 13.096686989642398 36.36149517113022 35.288338226613675 15.253479612613704 82-83 12.900571235202394 35.45099894059038 32.66673203858481 18.98169778562241 84-85 14.154129537569087 31.659804052579517 32.964774264380004 21.221292145471395 86-87 16.548291767528873 31.63984164553287 34.56928392479124 17.24258266214701 88-89 18.682717395275148 28.45380031955332 35.87880046375608 16.984681821415457 90-91 14.468934369415143 30.697047974381874 38.34258955721058 16.491428098992404 92-93 12.524706787156317 34.70534512593732 35.541530474908676 17.228417611997692 94-95 12.383364000453758 35.85313237236613 32.913703299935975 18.849800327244132 96-97 13.114087456090258 32.8376603775036 30.514842309474506 23.533409856931634 98-99 15.3231776135789 31.754697645265058 31.943987802369158 20.97813693878689 100-101 17.721461792997896 32.43889167680893 32.67028904001339 17.16935749017979 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 12949.0 1 7747.5 2 4517.5 3 5981.5 4 4053.5 5 3506.0 6 7917.5 7 16294.5 8 21508.5 9 20133.0 10 13417.5 11 7367.0 12 6138.5 13 5684.0 14 4826.5 15 3561.0 16 2394.5 17 1861.5 18 1707.5 19 1602.0 20 1529.0 21 1492.5 22 1518.5 23 1633.0 24 1668.5 25 1707.5 26 1994.5 27 2284.5 28 2435.5 29 2742.5 30 3279.5 31 3911.0 32 4918.5 33 6031.0 34 6927.5 35 7795.0 36 9629.5 37 11264.0 38 11114.5 39 10656.5 40 9782.5 41 8395.5 42 7293.0 43 6973.0 44 8238.5 45 8575.0 46 9620.0 47 16691.0 48 69388.0 49 72108.5 50 26461.0 51 17244.0 52 6086.0 53 2519.0 54 1685.0 55 1400.5 56 1194.0 57 960.5 58 746.0 59 682.5 60 644.5 61 564.5 62 461.5 63 274.0 64 124.0 65 108.0 66 114.0 67 89.5 68 56.0 69 49.5 70 50.0 71 46.5 72 44.0 73 57.5 74 54.0 75 31.0 76 20.0 77 18.0 78 12.0 79 4.0 80 4.0 81 2.5 82 0.5 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014804816756745326 3 0.006537191814666767 4 0.0 5 0.0 6 0.0 7 0.0 8 5.76811042470597E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 9.61351737450995E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005960380772196169 26-27 0.022303360308863086 28-29 0.0 30-31 0.0 32-33 8.652165637058956E-4 34-35 0.020476792007706194 36-37 0.004999029034745174 38-39 0.01778500714284341 40-41 0.01586230366794142 42-43 0.00980578772200015 44-45 0.003749271776058881 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 3.84540694980398E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0024033793436274878 86-87 3.84540694980398E-4 88-89 0.0 90-91 9.61351737450995E-5 92-93 1.92270347490199E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 520101.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.910751950102 #Duplication Level Percentage of deduplicated Percentage of total 1 72.92655173154132 13.06169378639918 2 11.34680206969105 4.064595145942807 3 4.569852072911522 2.455484607797332 4 2.4604418489812567 1.7627345457901444 5 1.6274126714902204 1.4574092339757085 6 1.0906670674367176 1.1720800383002532 7 0.757884792923546 0.9502000572965635 8 0.5968611117074951 0.8552185056364052 9 0.4970264293535436 0.8011905379916593 >10 3.438392339566739 12.256657841457717 >50 0.3746484316293449 4.631984941386384 >100 0.2329475921592202 8.591792747947032 >500 0.03220473624321017 4.148040476753553 >1k 0.03435171865942418 14.047656128328919 >5k 0.006440947248642034 7.518924208951723 >10k+ 0.00751443845674904 22.224337196044612 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 21729 4.177842380614535 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19897 3.82560310401249 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 17943 3.449906845016641 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16832 3.23629448895503 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13830 2.6590989057894525 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 12716 2.4449097386853706 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12642 2.430681732971096 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8206 1.577770471504573 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7248 1.3935754786089625 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6945 1.335317563319432 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 6043 1.1618897098832726 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5543 1.065754536138173 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5121 0.9846164494973091 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4721 0.9077083105012296 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4469 0.8592561829336993 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4279 0.8227248169105617 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4016 0.7721577155206393 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3442 0.661794536061265 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3418 0.6571800477215003 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3402 0.6541037221616571 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3399 0.6535269111191865 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3300 0.6344921467176567 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3075 0.591231318532362 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2911 0.5596989815439694 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2895 0.5566226559841262 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2854 0.548739571737028 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2124 0.4083822180691827 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 2035 0.39127015714255503 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1865 0.35858419806922115 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1686 0.32416780586847554 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1645 0.3162847216213774 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1627 0.3128238553665538 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1572 0.30224898625459284 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1375 0.2643717277990237 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1369 0.26321810571408244 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1361 0.26167994293416086 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1289 0.24783647791486654 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1214 0.2334162018531016 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1185 0.22784036177588585 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1149 0.22091862926623868 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1126 0.2164964112739641 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1101 0.21168965258670913 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1074 0.20649835320447374 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1049 0.20169159451721877 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1035 0.19899980965235597 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 990 0.19034764401529702 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 986 0.18957856262533623 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 967 0.18592542602302245 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 956 0.18381045220063028 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 897 0.1724665016987085 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 875 0.16823655405392415 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 873 0.16785201335894373 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 813 0.1563157925095318 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 809 0.155546711119571 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 801 0.15400854833964941 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 799 0.15362400764466902 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 796 0.15304719660219843 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 776 0.14920178965239445 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 772 0.14843270826243363 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 758 0.14574092339757086 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 692 0.13305108046321773 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 676 0.12997475490337454 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 671 0.12901340316592355 No Hit GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA 667 0.12824432177596273 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 647 0.12439891482615877 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 606 0.1165158305790606 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 556 0.10690231320455065 No Hit TTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCG 536 0.10305690625474669 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 530 0.10190328416980549 No Hit CATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 529 0.10171101382231529 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 523 0.10055739173737409 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 521 0.10017285104239369 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 521 0.10017285104239369 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.8840552123529856E-4 0.0 12-13 0.0 0.0 0.0 4.8067586872549753E-4 0.0 14-15 0.0 0.0 0.0 9.613517374509951E-4 0.0 16-17 0.0 0.0 0.0 0.0013458924324313932 0.0 18-19 0.0 0.0 0.0 0.0019227034749019901 0.0 20-21 0.0 0.0 0.0 0.0033647310810784826 0.0 22-23 0.0 0.0 0.0 0.007594678725862862 0.0 24-25 0.0 0.0 0.0 0.016439114710412015 0.0 26-27 0.0 0.0 0.0 0.030378714903451447 0.0 28-29 0.0 0.0 0.0 0.056623617335863616 0.0 30-31 0.0 0.0 0.0 0.12103418374508028 0.0 32-33 0.0 0.0 0.0 0.20861332702686594 0.0 34-35 0.0 0.0 0.0 0.3507972489958681 0.0 36-37 0.0 0.0 0.0 0.5442212185710084 0.0 38-39 0.0 0.0 0.0 0.7503350310805017 0.0 40-41 0.0 0.0 0.0 0.9638512519683677 0.0 42-43 0.0 0.0 0.0 1.1507380297288412 0.0 44-45 0.0 0.0 0.0 1.338009348184295 0.0 46-47 0.0 0.0 0.0 1.587095583357848 0.0 48-49 0.0 0.0 0.0 1.8851146219676562 0.0 50-51 0.0 0.0 0.0 2.1236259880292483 0.0 52-53 0.0 0.0 0.0 2.3202224183379765 0.0 54-55 0.0 0.0 0.0 2.499610652546332 0.0 56-57 0.0 0.0 0.0 2.693515297990198 0.0 58-59 0.0 0.0 0.0 3.00951161409034 0.0 60-61 0.0 0.0 0.0 3.472787016367975 0.0 62-63 0.0 0.0 0.0 3.8181045604603723 0.0 64-65 0.0 0.0 0.0 4.02354542675365 0.0 66-67 0.0 0.0 0.0 4.262441333510222 0.0 68-69 0.0 0.0 0.0 4.51277732594246 0.0 70-71 0.0 0.0 0.0 4.846270243664211 0.0 72-73 0.0 0.0 0.0 5.225523504088629 0.0 74-75 0.0 0.0 0.0 5.566226559841262 0.0 76-77 0.0 0.0 0.0 5.830117611771559 0.0 78-79 0.0 0.0 0.0 6.120541971655506 0.0 80-81 0.0 0.0 0.0 6.421925741346392 0.0 82-83 0.0 0.0 0.0 6.73292302841179 0.0 84-85 0.0 0.0 0.0 7.301850986635288 0.0 86-87 0.0 0.0 0.0 7.904906931538298 0.0 88-89 0.0 0.0 0.0 8.267624942078559 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATAACG 40 7.5211574E-6 59.399647 2 AGGCTTA 105 0.0 58.805656 6 GACATAT 45 1.511094E-5 52.799686 1 CGTCACT 40 5.374433E-4 47.51972 1 AGTGCGG 20 5.3871365E-4 47.501442 18-19 GTATAGG 115 1.8189894E-12 45.453644 1 GACAATG 105 2.0008883E-11 45.23512 7 GTCACTA 55 4.947414E-5 43.19974 2 ATAACGC 55 4.9586593E-5 43.18313 3 ATATAGG 195 0.0 41.427444 2 CTTACTA 150 0.0 41.163956 9 GGCTTAC 155 0.0 39.836086 7 CATATAG 60 8.283007E-5 39.584534 3 GATATAG 185 0.0 38.529503 1 GGCCGAG 25 0.0016147413 38.00481 26-27 GGGCCGA 25 0.0016147413 38.00481 26-27 CGAGAGG 25 0.0016147413 38.00481 30-31 GTGCGGG 25 0.0016147413 38.00481 20-21 CCTCGTC 50 0.0016155242 37.9975 8 AAAGTCA 125 1.3460522E-10 37.997498 9 >>END_MODULE