##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938044_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 533249 Sequences flagged as poor quality 0 Sequence length 101 %GC 33 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.02864890510812 33.0 31.0 34.0 31.0 34.0 2 31.98550770840639 33.0 31.0 34.0 30.0 34.0 3 31.935454168690423 33.0 31.0 34.0 30.0 34.0 4 35.48082978120915 37.0 35.0 37.0 33.0 37.0 5 35.551198408248304 37.0 35.0 37.0 35.0 37.0 6 35.70709743478187 37.0 35.0 37.0 35.0 37.0 7 35.70230605214449 37.0 35.0 37.0 35.0 37.0 8 35.74404452704084 37.0 36.0 37.0 35.0 37.0 9 37.27054340467586 39.0 37.0 39.0 34.0 39.0 10-11 37.201205253080644 39.0 37.0 39.0 34.0 39.0 12-13 37.02476891658493 39.0 37.0 39.0 33.5 39.0 14-15 37.81856881119327 40.0 37.5 41.0 33.0 41.0 16-17 37.7270046451095 40.0 37.5 41.0 32.5 41.0 18-19 38.220182316328774 40.0 38.0 41.0 34.0 41.0 20-21 38.30326076560856 40.0 38.0 41.0 34.0 41.0 22-23 38.186367906925284 40.0 38.0 41.0 34.0 41.0 24-25 38.007067054978066 40.0 37.5 41.0 34.0 41.0 26-27 37.548381712858344 40.0 36.0 41.0 33.5 41.0 28-29 37.23150066854322 39.0 35.0 41.0 33.0 41.0 30-31 36.882193871906 38.5 35.0 40.5 33.0 41.0 32-33 36.38044234494579 38.0 35.0 40.0 33.0 41.0 34-35 36.09523130845065 38.0 35.0 40.0 32.0 41.0 36-37 35.66637255766068 37.0 35.0 40.0 31.0 41.0 38-39 35.16759618864733 36.0 35.0 40.0 30.0 41.0 40-41 34.820655078584295 35.5 35.0 40.0 29.5 41.0 42-43 34.68639884931805 36.0 35.0 40.0 28.0 41.0 44-45 34.27664093134727 35.5 34.5 40.0 24.5 41.0 46-47 33.65861258061431 35.0 33.0 40.0 22.5 41.0 48-49 33.150259072215796 35.0 33.0 40.0 20.0 41.0 50-51 32.373921001258324 35.0 32.0 39.0 18.5 40.5 52-53 32.29729263439781 35.0 33.0 39.0 15.0 40.0 54-55 32.46705572818702 35.0 33.0 39.0 12.5 41.0 56-57 32.33375777544824 35.0 33.0 39.0 8.0 41.0 58-59 32.01184718583626 35.0 33.0 39.0 2.0 41.0 60-61 31.47349362117885 35.0 31.5 39.0 2.0 40.5 62-63 30.85443010676063 35.0 31.0 38.0 2.0 40.0 64-65 30.144856342909222 35.0 29.5 37.5 2.0 40.0 66-67 29.740034205408733 35.0 29.0 37.0 2.0 39.0 68-69 29.284322145939328 35.0 29.0 36.0 2.0 39.0 70-71 28.639056988386287 34.5 28.0 35.5 2.0 38.5 72-73 27.79223120905993 34.0 26.0 35.0 2.0 37.0 74-75 27.169889676305065 33.0 26.0 35.0 2.0 37.0 76-77 26.325647117950524 33.0 22.0 35.0 2.0 36.0 78-79 25.62536544841153 33.0 19.5 35.0 2.0 36.0 80-81 25.080573053114023 32.0 16.0 35.0 2.0 35.0 82-83 24.418977813366737 32.0 7.5 35.0 2.0 35.0 84-85 23.7142338757316 31.0 2.0 35.0 2.0 35.0 86-87 22.861002083454444 31.0 2.0 35.0 2.0 35.0 88-89 21.84390969322024 29.5 2.0 34.0 2.0 35.0 90-91 20.90415922017669 29.0 2.0 34.0 2.0 35.0 92-93 20.17103642013393 27.5 2.0 34.0 2.0 35.0 94-95 19.288100868449824 26.5 2.0 34.0 2.0 35.0 96-97 18.043982267196 24.0 2.0 33.0 2.0 35.0 98-99 16.72393197174303 10.0 2.0 33.0 2.0 35.0 100-101 13.436365562804617 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3383603446793657 1101 2 0.2175959848293516 1101 3 0.22409108683620715 1101 4 -0.10377328580721468 1101 5 0.1243099438873898 1101 6 0.22007395205152847 1101 7 0.32523593768046766 1101 8 0.297038626127204 1101 9 0.3563643122099194 1101 10-11 0.4676419480265821 1101 12-13 0.5826694389064144 1101 14-15 0.5467865127454274 1101 16-17 0.34013551259401 1101 18-19 0.5548616773835775 1101 20-21 0.6273679178179137 1101 22-23 0.5593136638098244 1101 24-25 0.607154219798332 1101 26-27 0.4703707408728235 1101 28-29 0.42394284476609556 1101 30-31 0.5803815364435962 1101 32-33 0.641333753297836 1101 34-35 0.7703075313791174 1101 36-37 0.9015246601893878 1101 38-39 0.49125201181518463 1101 40-41 0.5937140138500538 1101 42-43 0.628765554985911 1101 44-45 0.7247085120499932 1101 46-47 0.9026214541137279 1101 48-49 1.1968070994139666 1101 50-51 1.0922147076803626 1101 52-53 1.1411995349543318 1101 54-55 1.0448535465518134 1101 56-57 1.058351841595659 1101 58-59 0.4854310834237978 1101 60-61 0.6266798663448263 1101 62-63 0.665963731025407 1101 64-65 0.6813674566477381 1101 66-67 0.8307514866292394 1101 68-69 0.7054557774552528 1101 70-71 0.4274373673059415 1101 72-73 0.5766118187652118 1101 74-75 0.9657568784569683 1101 76-77 0.8802103178191167 1101 78-79 0.8670007042044681 1101 80-81 0.7254199460202706 1101 82-83 0.6301849360913323 1101 84-85 0.36569973620197516 1101 86-87 0.629164585588903 1101 88-89 0.3486351994618033 1101 90-91 0.6091896224274507 1101 92-93 0.03158285262255944 1101 94-95 0.22361732142444168 1101 96-97 -0.2469219832540297 1101 98-99 -0.19676701550643472 1101 100-101 -0.2672887378947362 1102 1 0.1938650337766603 1102 2 0.19455792387849868 1102 3 0.15305089245719827 1102 4 0.11146591000889572 1102 5 0.18317181922999737 1102 6 -0.04894541177204559 1102 7 0.03301554269994966 1102 8 0.220975986306172 1102 9 0.3186589327115712 1102 10-11 0.2748630097034024 1102 12-13 0.24943981388604186 1102 14-15 0.2829377490966749 1102 16-17 0.27007313945149747 1102 18-19 0.29106476789130653 1102 20-21 0.32273215622851126 1102 22-23 0.5468094043612552 1102 24-25 0.3874629742614957 1102 26-27 0.4572960857029642 1102 28-29 0.576816305808812 1102 30-31 0.5202307149256029 1102 32-33 0.5540706156768991 1102 34-35 0.6111226787189992 1102 36-37 0.7538440225314176 1102 38-39 0.575174260211412 1102 40-41 0.6708581832205383 1102 42-43 0.5435173217311089 1102 44-45 0.6590167035251611 1102 46-47 0.3440632959733634 1102 48-49 0.4890719408143269 1102 50-51 0.697286044184203 1102 52-53 0.9129227226515582 1102 54-55 0.6799706628680937 1102 56-57 0.8653136324900785 1102 58-59 0.8086602639799736 1102 60-61 0.6549329738903431 1102 62-63 0.5993607708938455 1102 64-65 0.5319682240892547 1102 66-67 0.41198472662508934 1102 68-69 0.30750475740991234 1102 70-71 -0.30925463382003215 1102 72-73 -0.10908532332999599 1102 74-75 -0.39004412010593015 1102 76-77 -0.6776737362869909 1102 78-79 -0.4348349329349581 1102 80-81 -0.45138050437011756 1102 82-83 -0.646405134578476 1102 84-85 -0.8365018431274756 1102 86-87 -0.72733274021817 1102 88-89 -0.5963401327751825 1102 90-91 -1.503082034090614 1102 92-93 -1.528671677208397 1102 94-95 -1.745492416786142 1102 96-97 -1.519985971283127 1102 98-99 -1.3044162443610539 1102 100-101 -1.4704310997774854 1103 1 0.480028201312237 1103 2 0.4824777021885822 1103 3 0.5655209502280805 1103 4 0.35671507632329735 1103 5 0.3974595464386752 1103 6 0.2615942528749855 1103 7 0.3881280315450013 1103 8 0.42097598630617483 1103 9 0.4592535501555588 1103 10-11 0.5429739485977692 1103 12-13 0.34830083497476494 1103 14-15 0.5283325168389084 1103 16-17 0.3213483261501011 1103 18-19 0.31089004617791005 1103 20-21 0.5199211093072762 1103 22-23 0.4851002314713 1103 24-25 0.4371318484336797 1103 26-27 0.5818158358321242 1103 28-29 0.3839742598739022 1103 30-31 0.39537749055850213 1103 32-33 0.45083920334305816 1103 34-35 0.24475895695862704 1103 36-37 0.1665301340339269 1103 38-39 0.38128247495003365 1103 40-41 0.6092335530951303 1103 42-43 0.5095452208434068 1103 44-45 0.6568209400570382 1103 46-47 0.6697783868568408 1103 48-49 0.5979090081197569 1103 50-51 0.696377209465318 1103 52-53 0.7474349578683217 1103 54-55 0.3373305802040605 1103 56-57 0.42900251511652954 1103 58-59 0.6020001728616577 1103 60-61 0.5934774292940368 1103 62-63 0.6530430779719545 1103 64-65 0.7456970296878644 1103 66-67 0.5103150070252589 1103 68-69 0.406607664741653 1103 70-71 -0.18039737018751367 1103 72-73 0.02775418632197102 1103 74-75 0.15313609688716312 1103 76-77 0.5078365732334618 1103 78-79 -0.06662536081528359 1103 80-81 -0.19358143434052622 1103 82-83 -0.12186894557843075 1103 84-85 -0.43562588615035125 1103 86-87 -0.33212819369616753 1103 88-89 0.21257208365429392 1103 90-91 0.04367246311477757 1103 92-93 -0.32330790847982627 1103 94-95 -0.09097642411318319 1103 96-97 0.011924225513642028 1103 98-99 -0.32641145360439694 1103 100-101 -0.4477994713899047 1104 1 0.10472194728257733 1104 2 0.13579814061539963 1104 3 0.11409176475634197 1104 4 0.20941226093538035 1104 5 0.21585627972903154 1104 6 0.20204127630073287 1104 7 0.11714157119683932 1104 8 0.12602649135667576 1104 9 0.26213341260989154 1104 10-11 0.1825290743231065 1104 12-13 0.252115578151205 1104 14-15 0.13226546332504796 1104 16-17 0.025173815081970474 1104 18-19 0.08415460667651331 1104 20-21 0.04959014004013085 1104 22-23 0.21726227746314208 1104 24-25 0.26137427267610747 1104 26-27 0.14535548766965434 1104 28-29 0.4391214767232512 1104 30-31 0.38747936031994357 1104 32-33 0.3236739336245975 1104 34-35 0.4311440848331216 1104 36-37 0.34658601196214534 1104 38-39 0.5632403515887674 1104 40-41 0.5201190033422876 1104 42-43 0.323837075560796 1104 44-45 0.6750779785268364 1104 46-47 0.3526926357285376 1104 48-49 0.22441878675719806 1104 50-51 0.09176728038729465 1104 52-53 0.0598355596306277 1104 54-55 0.0027614849966610677 1104 56-57 0.21174483190140592 1104 58-59 0.3175170436976842 1104 60-61 0.40625413249626874 1104 62-63 0.23895539260767507 1104 64-65 0.3300082267650204 1104 66-67 -0.04219949974456583 1104 68-69 -0.09186643798777183 1104 70-71 -0.42058649548302185 1104 72-73 -0.588976954877257 1104 74-75 -0.37449124031464365 1104 76-77 -1.017308494464796 1104 78-79 -1.0731050513375315 1104 80-81 -0.9911958766358175 1104 82-83 -0.9160017631154993 1104 84-85 -1.2166811193332414 1104 86-87 -1.1646663411279299 1104 88-89 -1.4343761218045508 1104 90-91 -1.566310343676541 1104 92-93 -1.8661189980994273 1104 94-95 -1.769420438727412 1104 96-97 -2.0834550996529195 1104 98-99 -2.6919605617066953 1104 100-101 -2.1464562519615775 1105 1 0.2834198170695572 1105 2 0.08713327412875316 1105 3 0.2925696125411257 1105 4 0.2682381435236394 1105 5 0.2710217962806851 1105 6 0.2919502671998231 1105 7 0.3616060176414848 1105 8 0.258599748682407 1105 9 0.27033423269189427 1105 10-11 0.29369019043471667 1105 12-13 0.36640700729412146 1105 14-15 0.4420464414228533 1105 16-17 0.42699399710017616 1105 18-19 0.26905309652549647 1105 20-21 0.06202138316444916 1105 22-23 0.16309686092147757 1105 24-25 0.19950808605744186 1105 26-27 0.19594054617549972 1105 28-29 0.11885945052063107 1105 30-31 -0.08080746836292008 1105 32-33 -0.09851828559732922 1105 34-35 -0.20441947152251316 1105 36-37 -0.10578922556160819 1105 38-39 -0.26387235968235956 1105 40-41 0.1600129927412226 1105 42-43 0.3608407759308321 1105 44-45 -0.12842237150815805 1105 46-47 0.19385675214017795 1105 48-49 0.3004263875172697 1105 50-51 0.35084318797805736 1105 52-53 0.22445202127678954 1105 54-55 0.4316743762857911 1105 56-57 0.4177954369619101 1105 58-59 0.18428372036535023 1105 60-61 0.13932743982700302 1105 62-63 -0.39440794372596244 1105 64-65 -0.39563433749140486 1105 66-67 -0.23184846464105746 1105 68-69 -0.1410013514791224 1105 70-71 -0.42663710054352677 1105 72-73 -0.5682748846702346 1105 74-75 -0.5524990410947233 1105 76-77 -0.5949862622415765 1105 78-79 0.06487874704231089 1105 80-81 -0.025159272975454172 1105 82-83 -0.12431259419861007 1105 84-85 -0.07675712693400172 1105 86-87 0.36559668517469746 1105 88-89 0.20299761556918483 1105 90-91 -0.016165329175091614 1105 92-93 -0.01235362156177544 1105 94-95 -0.5114946461481509 1105 96-97 -0.834210175160468 1105 98-99 -0.5886402296734907 1105 100-101 -0.6697185781942032 1106 1 0.09969607884207932 1106 2 0.11347737195316654 1106 3 0.20017208010495224 1106 4 0.07213707000165215 1106 5 0.029209322350361333 1106 6 0.12492868699302306 1106 7 0.27589988605272 1106 8 0.061330753490210554 1106 9 0.4079821438244764 1106 10-11 0.2927286308641257 1106 12-13 0.3074247676067401 1106 14-15 0.36567270648838246 1106 16-17 -0.01794025242233488 1106 18-19 0.3878254615668837 1106 20-21 0.43668055639884784 1106 22-23 0.4049686090719078 1106 24-25 0.49810745818977864 1106 26-27 0.561763469931293 1106 28-29 0.4043592200097734 1106 30-31 0.4403248887752298 1106 32-33 0.7458715192368146 1106 34-35 1.0048075487404873 1106 36-37 0.7194857409594348 1106 38-39 0.6354009822859794 1106 40-41 0.5770049358379765 1106 42-43 0.7534995540037599 1106 44-45 1.0240309226017317 1106 46-47 0.9277111131860352 1106 48-49 0.6896318934337131 1106 50-51 0.7479387511929119 1106 52-53 0.8700843893916499 1106 54-55 1.1002239141664134 1106 56-57 1.0628657976565208 1106 58-59 1.2661743484525445 1106 60-61 1.4164783256472937 1106 62-63 0.992022650552979 1106 64-65 0.9290720355849267 1106 66-67 1.106137529080268 1106 68-69 0.9060887233820232 1106 70-71 0.5653213635955723 1106 72-73 0.10969266818261403 1106 74-75 0.6938753524421948 1106 76-77 0.4822480465551564 1106 78-79 0.116473662631428 1106 80-81 -0.2263030458893347 1106 82-83 -0.17549917627145106 1106 84-85 0.4564649757640744 1106 86-87 0.2030843363788435 1106 88-89 -0.029153160484028717 1106 90-91 0.1223321791123233 1106 92-93 -0.11260828117407584 1106 94-95 -0.04616347888508443 1106 96-97 -0.3839478318529679 1106 98-99 -0.9883636166463354 1106 100-101 -1.1198733497689197 1107 1 -0.08993597211760829 1107 2 0.007432005032768529 1107 3 -0.16349857159687886 1107 4 -0.00785317694861476 1107 5 -0.03642946430929328 1107 6 -0.0943244427453962 1107 7 0.0306373537647886 1107 8 -0.09861164255980981 1107 9 -0.23265988115247893 1107 10-11 0.04341629706599548 1107 12-13 -0.038366562846391616 1107 14-15 -0.1418363798489608 1107 16-17 -0.05953929540433478 1107 18-19 0.042834185974648165 1107 20-21 -0.022116618264561794 1107 22-23 0.3068228314566781 1107 24-25 0.3168569343443295 1107 26-27 0.2306569559608036 1107 28-29 0.18667953149779493 1107 30-31 0.24947561149146225 1107 32-33 0.00743421686940593 1107 34-35 0.028483457945682744 1107 36-37 -0.23118135240316207 1107 38-39 -0.32296187688150724 1107 40-41 0.04181014152827345 1107 42-43 -0.24647949217845877 1107 44-45 -0.27368282538860456 1107 46-47 -0.4893098634904547 1107 48-49 -0.47602898997924115 1107 50-51 -1.1458615855920833 1107 52-53 -1.1177547767225988 1107 54-55 -1.0080997042150344 1107 56-57 -1.229091365120361 1107 58-59 -1.027432451251812 1107 60-61 -0.8750902390542876 1107 62-63 -0.9793982468799847 1107 64-65 -0.9590993437858479 1107 66-67 -0.7388880345774318 1107 68-69 -0.8475781947304561 1107 70-71 -0.7864513293890916 1107 72-73 -0.947817942068756 1107 74-75 -1.3130260731793726 1107 76-77 -1.5252591348896622 1107 78-79 -1.253205020097294 1107 80-81 -1.3867597629213542 1107 82-83 -1.384667804977422 1107 84-85 -1.57234080901355 1107 86-87 -1.5716068805426993 1107 88-89 -1.6756965425064152 1107 90-91 -1.4205850494976389 1107 92-93 -1.3535448025355414 1107 94-95 -1.3909033086638871 1107 96-97 -1.5153149351210011 1107 98-99 -1.6404349967790672 1107 100-101 -1.5598635205234856 1108 1 0.10311158876019277 1108 2 0.06978432199834828 1108 3 0.09365036693613504 1108 4 0.0995089187160545 1108 5 0.04803443470790114 1108 6 0.19397134902074953 1108 7 0.011249617549054847 1108 8 0.0019454656598512088 1108 9 0.20230714272975803 1108 10-11 0.16358015968298645 1108 12-13 0.13119361162780763 1108 14-15 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sequence quality scores pass #Quality Count 2 2424.0 3 414.0 4 371.0 5 361.0 6 404.0 7 532.0 8 700.0 9 850.0 10 1156.0 11 1672.0 12 2668.0 13 3688.0 14 4377.0 15 5722.0 16 7243.0 17 9466.0 18 9258.0 19 6621.0 20 6401.0 21 6804.0 22 7632.0 23 8688.0 24 9579.0 25 10732.0 26 12402.0 27 13941.0 28 16200.0 29 18206.0 30 21289.0 31 24593.0 32 29051.0 33 37295.0 34 54681.0 35 93048.0 36 48913.0 37 38649.0 38 16375.0 39 843.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 55.586981426183634 17.5882209326347 13.49972768418878 13.325069956992882 2 13.75421440806915 26.072950301411087 40.12529245224609 20.047542838273674 3 23.940265769250992 35.88354379032924 29.58711116786731 10.589079272552452 4 10.524606169849228 18.903096190315253 37.8996977036745 32.67259993616102 5 9.493848058601726 45.55724433606189 30.654685301284495 14.294222304051885 6 20.469422878916554 39.08005591588567 19.30075080604553 21.149770399152242 7 21.728351004385676 44.403331987265574 18.314097085919816 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37.23246993498467 36.84455544699331 12.026006132671824 32-33 13.238058043886841 36.105911223683194 34.72606867856935 15.92996205386061 34-35 14.559414661432207 32.27527280542617 35.31599629470776 17.849316238433865 36-37 17.822415882022266 32.42698419699744 36.68353734244156 13.067062578538735 38-39 20.354193168300856 29.065960456747458 37.97238576623026 12.60746060872143 40-41 14.064379220477067 32.33023808228277 41.04897676591609 12.556405931324077 42-43 12.66727024529373 36.431443006384484 36.93131869420612 13.969968054115672 44-45 13.281418545623044 37.37864715140896 33.50135915190422 15.838575151063777 46-47 13.195927158654694 34.328622836279585 31.518459870092347 20.956990134973378 48-49 15.608306044554423 33.54488976771629 33.308413073474746 17.538391114254544 50-51 18.99114051348248 34.51147117758749 33.34512221663527 13.152266092294763 52-53 13.540220743264028 34.49188532154835 37.14566279252984 14.822231142657774 54-55 11.325599131708133 34.0156341316753 37.697807037704465 16.960959698912102 56-57 14.825760247300776 37.46473562976913 31.43555999069621 16.273944132233886 58-59 13.263910516421706 33.85530211213965 33.583264229457384 19.297523141981262 60-61 14.341818195461359 31.20517683518207 35.31760547923619 19.135399490120378 62-63 20.472938048830365 30.459833192153596 33.361944509327714 15.705284249688326 64-65 17.877306149325072 31.533218211509617 36.84989546175513 13.739580177410172 66-67 13.795328707876815 32.65420976260847 37.815498874901635 15.73496265461309 68-69 13.115535411551571 36.82221561318759 35.71730802969562 14.344940945565218 70-71 13.906590205733444 35.33847846745422 32.196988529921924 18.557942796890405 72-73 13.73218560739668 33.442746287026544 31.31880914678534 21.506258958791435 74-75 15.564541773249024 33.41390912663907 33.899427527279926 17.12212157283198 76-77 18.10785768277792 30.219339131609107 35.70835060499262 15.964452580620353 78-79 14.773490342015327 30.60112467034554 38.40075853350189 16.224626454137233 80-81 13.746423182001067 36.36504317504204 35.067869271581195 14.820664371375702 82-83 13.780791803982842 34.801842678335966 32.67649778574738 18.740867731933818 84-85 15.124025846142302 31.24335324624171 33.069557633333645 20.56306327428234 86-87 17.797187188163534 31.15157221851249 34.51612358665396 16.53511700667002 88-89 20.12889170769785 27.967567851567193 35.69534721388255 16.20819322685241 90-91 15.801819052178075 30.34766611287885 37.993129276602936 15.85738555834014 92-93 14.312148361114135 34.307381648531894 35.134331215211446 16.246138775142533 94-95 14.434989204511867 35.07412338853529 32.51445262981093 17.976434777141904 96-97 15.270712260442215 31.822975404806446 30.35630848794072 22.55000384681062 98-99 17.415524788589163 31.022089547758114 31.613308141029368 19.94907752262335 100-101 20.355888190197586 30.47440492843369 32.70532480383339 16.464382077535333 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13646.0 1 8324.5 2 4786.0 3 6075.5 4 4167.5 5 3664.5 6 7887.0 7 16004.0 8 21450.5 9 20107.0 10 13633.5 11 7877.0 12 6375.0 13 5912.0 14 5108.5 15 3828.5 16 2651.0 17 2007.5 18 1739.0 19 1623.0 20 1564.0 21 1551.5 22 1604.5 23 1686.0 24 1716.5 25 1797.5 26 2112.5 27 2375.0 28 2511.5 29 2885.0 30 3523.0 31 4107.5 32 5100.0 33 6286.0 34 7234.0 35 8063.5 36 9698.0 37 11274.0 38 11253.5 39 10745.0 40 9792.0 41 8443.5 42 7319.5 43 7148.5 44 8287.0 45 9159.0 46 11625.0 47 21113.0 48 60440.0 49 65746.0 50 31527.0 51 18012.5 52 8763.5 53 4641.0 54 3028.0 55 2162.5 56 1583.0 57 1247.5 58 1056.5 59 922.5 60 812.5 61 719.5 62 594.0 63 414.5 64 266.5 65 202.5 66 176.5 67 140.0 68 116.5 69 107.5 70 92.0 71 79.0 72 68.0 73 74.0 74 72.5 75 56.0 76 37.5 77 18.5 78 13.0 79 11.0 80 7.5 81 8.0 82 5.0 83 1.5 84 1.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14608559978546606 2 0.04800759120035856 3 0.14195994741668527 4 0.12358204141029801 5 0.1834040007576198 6 0.19146777584205502 7 0.1563997307074181 8 0.06394761171610261 9 0.11176767326333475 10-11 0.09929695133042912 12-13 0.14064723984480046 14-15 0.12808275308533162 16-17 0.12498851380874601 18-19 0.12686381034001 20-21 0.12686381034001 22-23 0.1849980028091942 24-25 0.20215696607025985 26-27 0.14458536256045487 28-29 0.1224568634915396 30-31 0.09910942167730272 32-33 0.12226933383841321 34-35 0.0932960024303843 36-37 0.0882327017959715 38-39 0.04932029877224336 40-41 0.05288336218164497 42-43 0.05935313521450579 44-45 0.036755812012774516 46-47 0.04782006154723216 48-49 0.040412640248739334 50-51 0.04894523946599056 52-53 0.026441681090822487 54-55 0.04660111880191055 56-57 0.02494144386581128 58-59 0.044069468484704145 60-61 0.03413039686900491 62-63 0.04510088157689934 64-65 0.03572439892057932 66-67 0.03459922100182091 68-69 0.05785289798949459 70-71 0.04753876706754255 72-73 0.047445002240979355 74-75 0.03947499198310733 76-77 0.01912802461889286 78-79 0.02156591010953607 80-81 0.02409756042674248 82-83 0.021003321150156868 84-85 0.017627787393881655 86-87 0.021940969415788873 88-89 0.0601970186535746 90-91 0.10389142783202593 92-93 0.07257397575991703 94-95 0.07266774058648023 96-97 0.0637600820629762 98-99 0.05288336218164497 100-101 0.12489474898218281 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 533249.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.930997998208852 #Duplication Level Percentage of deduplicated Percentage of total 1 74.32851360480393 14.071129422623823 2 10.712285684434915 4.055885176965574 3 4.384223646644457 2.4899318723497834 4 2.3641227391288893 1.7902081136787609 5 1.5144206876501503 1.4334747503175533 6 1.0086134918276468 1.1456435996853371 7 0.7587092266703217 1.005418599592291 8 0.5937724368626026 0.8992563850910016 9 0.49181151329240536 0.8379434496271133 >10 3.2167671743114106 12.122929826003674 >50 0.3388701280582666 4.523342880817909 >100 0.21291839904545953 8.201551288735647 >500 0.02798927311395712 3.653796776255373 >1k 0.033986974495519365 14.230278122066062 >5k 0.00599770138156224 7.330869759444629 >10k+ 0.00699731827848928 22.208339976745485 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 22573 4.233106860022241 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20603 3.863673443363232 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 17856 3.3485294862250097 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16352 3.0664848879229027 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 13700 2.5691562478316885 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 13569 2.54458986327213 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12704 2.382376713317793 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 8227 1.5428064562708979 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 7020 1.3164581649473321 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 6836 1.2819527087720746 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 5703 1.0694816117798627 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5641 1.057854773286026 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 5312 0.9961575174074401 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4526 0.8487592100500891 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4506 0.8450086169875611 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 4346 0.815003872487337 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 4212 0.7898748989683994 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 3567 0.668918272701871 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3546 0.6649801499862166 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3396 0.6368507020172565 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 3371 0.6321624606890964 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 3198 0.5997198306982292 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 3045 0.5710277937698899 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 2955 0.5541501249885139 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 2866 0.5374599858602641 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 2863 0.536897396900885 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2077 0.38949908954353407 No Hit GATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTT 1916 0.35930681539018355 No Hit GTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGC 1876 0.35180562926512754 No Hit CCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATA 1686 0.3161749951711114 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1658 0.3109241648835722 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 1655 0.310361575924193 No Hit TGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGT 1590 0.298172148470977 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1369 0.2567280951300424 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1364 0.25579044686441044 No Hit AAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTAC 1312 0.2460389049018376 No Hit GCTTATACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 1259 0.23609983328613837 No Hit GTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTT 1197 0.22447299479230154 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1188 0.22278522791416394 No Hit ATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGT 1169 0.2192221645047623 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 1154 0.2164092197078663 No Hit GCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAAC 1087 0.20384473294839744 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 1079 0.20234449572338625 No Hit GTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1075 0.20159437711088066 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1036 0.19428072063895102 No Hit AGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAG 1030 0.19315554272019264 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 1024 0.19203036480143423 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 989 0.1854668269420102 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 950 0.17815317047008059 No Hit GCTTATACTCTGCGTTGATACCACTGCTTATACTCTGCGTTGATACCACT 886 0.16615127266999094 No Hit GTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCAGA 878 0.16465103544497972 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 830 0.15564961209491252 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 796 0.14927360388861488 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 788 0.1477733666636037 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 781 0.14646065909171888 No Hit TACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGA 764 0.14327265498857006 No Hit CTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACT 737 0.13820935435415724 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 720 0.13502135025100845 No Hit GAGTACATGGGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 719 0.13483382059788204 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 712 0.13352111302599723 No Hit GCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAA 699 0.13108322753535404 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 691 0.12958299031034282 No Hit GTACATGGGGGGGAGGTAGTGACGAAAAAAAAAAAAAAAAAAAAAAAAAA 668 0.12526980828843562 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 610 0.11439308840710438 No Hit TCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTA 607 0.11383049944772518 No Hit GAGTATAAGCAGTGGTATCAACGCAGAGTATAAGCAGTGGTATCAACGCA 586 0.10989237673207075 No Hit ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 575 0.10782955054768036 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 573 0.10745449124142754 No Hit GTATAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 557 0.10445401679140515 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 553 0.10370389817889955 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 543 0.10182860164763555 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 538 0.10089095338200353 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 7.501186125056025E-4 0.0 18-19 0.0 0.0 0.0 7.501186125056025E-4 0.0 20-21 0.0 0.0 0.0 0.0022503558375168075 0.0 22-23 0.0 0.0 0.0 0.008063775084435227 0.0 24-25 0.0 0.0 0.0 0.017909081873571257 0.0 26-27 0.0 0.0 0.0 0.03263015964399371 0.0 28-29 0.0 0.0 0.0 0.059259370387942586 0.0 30-31 0.0 0.0 0.0 0.1211441559196548 0.0 32-33 0.0 0.0 0.0 0.20628261843904067 0.0 34-35 0.0 0.0 0.0 0.34749244724322037 0.0 36-37 0.0 0.0 0.0 0.5449611719853201 0.0 38-39 0.0 0.0 0.0 0.7443989580852473 0.0 40-41 0.0 0.0 0.0 0.9484312206867711 0.0 42-43 0.0 0.0 0.0 1.1311788676584484 0.0 44-45 0.0 0.0 0.0 1.3067066229847595 0.0 46-47 0.0 0.0 0.0 1.5436503397099666 0.0 48-49 0.0 0.0 0.0 1.8226944635620508 0.0 50-51 0.0 0.0 0.0 2.0465111045684097 0.0 52-53 0.0 0.0 0.0 2.2461364203214633 0.0 54-55 0.0 0.0 0.0 2.437041607204139 0.0 56-57 0.0 0.0 0.0 2.6356355098649975 0.0 58-59 0.0 0.0 0.0 2.9443093189110527 0.0 60-61 0.0 0.0 0.0 3.399350022222264 0.0 62-63 0.0 0.0 0.0 3.719744434588719 0.0 64-65 0.0 0.0 0.0 3.93305941502 0.0 66-67 0.0 0.0 0.0 4.171503368970218 0.0 68-69 0.0 0.0 0.0 4.419323805576757 0.0 70-71 0.0 0.0 0.0 4.746187990976074 0.0 72-73 0.0 0.0 0.0 5.120028354483553 0.0 74-75 0.0 0.0 0.0 5.450736897771961 0.0 76-77 0.0 0.0 0.0 5.713934765934864 0.0 78-79 0.0 0.0 0.0 5.990165944990052 0.0 80-81 0.0 0.0 0.0 6.291151038257924 0.0 82-83 0.0 0.0 0.0 6.6072322686024725 0.0 84-85 0.0 0.0 0.0 7.165789340439456 0.0 86-87 0.0 9.376482656320031E-5 0.0 7.7565077477876185 0.0 88-89 0.0 1.8752965312640062E-4 0.0 8.104562783990218 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCAGGA 25 0.00498951 57.08721 2 TATAACG 35 2.7656148E-4 54.36877 2 ATAACGC 45 9.5482834E-4 42.286827 3 GTAAGCG 65 5.456968E-12 40.2103 94-95 CCCTATA 60 8.210437E-5 39.643898 2 AAGCTGC 25 0.0016155664 38.00094 92-93 GTTCGCA 80 1.0694273E-5 35.659378 9 ATGAGAG 190 0.0 35.05355 5 CATGAGA 205 0.0 34.80928 4 CCTAGTC 55 0.002559124 34.598312 3 CTAGTCG 55 0.002559124 34.598312 4 TAGTCGA 55 0.002559124 34.598312 5 ACTATAT 55 0.0025662174 34.578793 8 CTGTTCG 85 1.6187896E-5 33.564922 7 TGTTCGC 85 1.619832E-5 33.561768 8 CCTATAC 100 1.2820437E-6 33.300873 3 CATGGAT 60 0.0039196126 31.715118 4 AAGACGG 30 0.0039648996 31.64367 82-83 ACGATGA 30 0.003969457 31.636244 54-55 GGGAACT 140 1.6849299E-8 30.568056 7 >>END_MODULE