##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938043_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 201489 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.333407779084716 33.0 31.0 34.0 31.0 34.0 2 32.515571569663855 34.0 31.0 34.0 31.0 34.0 3 32.51374516722997 34.0 31.0 34.0 30.0 34.0 4 36.03714346688901 37.0 35.0 37.0 35.0 37.0 5 36.00018363285341 37.0 35.0 37.0 35.0 37.0 6 36.12443855495834 37.0 36.0 37.0 35.0 37.0 7 36.09538485971939 37.0 36.0 37.0 35.0 37.0 8 36.138687471772656 37.0 36.0 37.0 35.0 37.0 9 37.721955044692265 39.0 38.0 39.0 35.0 39.0 10-11 37.742464849197724 39.0 38.0 39.0 35.0 39.0 12-13 37.683982748437884 39.0 37.0 39.0 35.0 39.0 14-15 38.69154891830323 40.0 38.0 41.0 34.0 41.0 16-17 38.639920789720534 40.0 38.0 41.0 34.5 41.0 18-19 38.929579282243694 40.0 38.0 41.0 35.0 41.0 20-21 38.95923102501874 40.0 38.0 41.0 35.0 41.0 22-23 38.80101146960877 40.0 38.0 41.0 35.0 41.0 24-25 38.6116711085965 40.0 38.0 41.0 35.0 41.0 26-27 38.32007454501238 40.0 37.0 41.0 34.5 41.0 28-29 38.06658179851009 40.0 36.5 41.0 34.0 41.0 30-31 37.61778062325983 39.5 35.5 41.0 33.5 41.0 32-33 37.07556740070177 39.0 35.0 41.0 33.0 41.0 34-35 36.651606291162295 38.5 35.0 41.0 33.0 41.0 36-37 36.40662269404285 38.5 35.0 41.0 32.5 41.0 38-39 36.03942398840631 38.0 35.0 41.0 31.0 41.0 40-41 35.804130746591625 38.0 35.0 41.0 31.0 41.0 42-43 35.38640570949283 38.0 35.0 40.0 29.5 41.0 44-45 34.88477782906263 38.0 35.0 40.0 26.0 41.0 46-47 34.219510742521926 38.0 34.5 40.0 22.5 41.0 48-49 33.539523249408155 37.0 33.5 40.0 17.5 41.0 50-51 33.02320474070545 37.0 33.0 40.0 9.0 41.0 52-53 32.575510325625714 36.0 33.0 40.0 3.5 41.0 54-55 32.226007375092436 36.0 33.0 40.0 2.0 41.0 56-57 31.758793284000618 35.0 32.5 40.0 2.0 41.0 58-59 31.188382492344495 35.0 31.0 39.0 2.0 41.0 60-61 30.598372615874812 35.0 30.5 39.0 2.0 40.5 62-63 30.061172073909745 35.0 29.5 39.0 2.0 40.0 64-65 29.485056256172793 35.0 28.0 38.0 2.0 40.0 66-67 28.857004600747434 35.0 27.5 38.0 2.0 40.0 68-69 28.279938358917857 35.0 26.0 37.0 2.0 39.5 70-71 27.696412707393456 34.0 25.5 36.5 2.0 39.0 72-73 27.075999186059782 34.0 23.5 36.0 2.0 38.5 74-75 26.547598131907947 33.0 21.5 35.0 2.0 38.0 76-77 25.262706648998208 31.5 20.0 34.5 2.0 36.0 78-79 25.922688087190863 33.0 20.0 35.0 2.0 36.0 80-81 25.649362992520686 33.0 18.0 35.0 2.0 36.0 82-83 25.172436212398694 33.0 11.5 35.0 2.0 36.0 84-85 24.636617879884263 32.5 4.5 35.0 2.0 35.0 86-87 24.09247154931535 32.0 2.0 35.0 2.0 35.0 88-89 23.416484274575787 31.0 2.0 35.0 2.0 35.0 90-91 22.898959744700704 31.0 2.0 34.0 2.0 35.0 92-93 22.15600107201882 30.5 2.0 34.0 2.0 35.0 94-95 21.4437090858558 30.0 2.0 34.0 2.0 35.0 96-97 20.60821186268233 29.0 2.0 34.0 2.0 35.0 98-99 19.48894232439488 28.0 2.0 34.0 2.0 35.0 100-101 16.07625974618962 13.0 2.0 30.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07449382577483021 1101 2 0.2019352582050189 1101 3 0.24036267370174613 1101 4 0.06583031872019518 1101 5 0.004731760093378057 1101 6 -0.013879839461907295 1101 7 0.11993092150029128 1101 8 -0.1156778794247657 1101 9 -0.12113171997686578 1101 10-11 0.1880025402674903 1101 12-13 0.23014591844617627 1101 14-15 0.3251556398012099 1101 16-17 0.4897064072015098 1101 18-19 0.2595607463018652 1101 20-21 0.059767047614293745 1101 22-23 0.20863972391067165 1101 24-25 0.2732315406873198 1101 26-27 0.4599873118246194 1101 28-29 0.32694688050555953 1101 30-31 0.6721391761005435 1101 32-33 1.1630411823896196 1101 34-35 0.6935115572378692 1101 36-37 0.9928498900594604 1101 38-39 0.9854346551440258 1101 40-41 1.194105630392663 1101 42-43 1.0249796472562096 1101 44-45 0.5102175270590052 1101 46-47 0.3661028998692828 1101 48-49 0.5695736500570661 1101 50-51 0.9276951097034001 1101 52-53 0.6602566899232514 1101 54-55 0.7915278947034068 1101 56-57 1.049914980890076 1101 58-59 1.034723661904966 1101 60-61 0.8775303890776698 1101 62-63 0.86389581940238 1101 64-65 0.7267448388542697 1101 66-67 0.5030826984499868 1101 68-69 0.845631692926613 1101 70-71 0.7837371649107823 1101 72-73 1.1643897991351437 1101 74-75 1.103804884521999 1101 76-77 1.0050901378463095 1101 78-79 1.1323241005663327 1101 80-81 1.1970014009776406 1101 82-83 1.0005484207411932 1101 84-85 1.4572257675386169 1101 86-87 1.8780450663112873 1101 88-89 2.470737318871148 1101 90-91 2.8097239322716163 1101 92-93 2.9374574762618586 1101 94-95 3.105872236187963 1101 96-97 3.941427784023407 1101 98-99 3.9887782427041927 1101 100-101 3.869515843655151 1102 1 0.04958961121543837 1102 2 0.1385252965191981 1102 3 0.37005616029178867 1102 4 0.3393935371110004 1102 5 0.05971260300525927 1102 6 0.03133088850744059 1102 7 0.022229772075000653 1102 8 0.2770424270886451 1102 9 0.3085617666131739 1102 10-11 0.38091441766212597 1102 12-13 0.4405865314729951 1102 14-15 0.6177801608740197 1102 16-17 0.054074223293469004 1102 18-19 0.24107415626355078 1102 20-21 0.3499969326717718 1102 22-23 0.4986780380869149 1102 24-25 0.40886372459536346 1102 26-27 0.24389535780163385 1102 28-29 0.15424573108027317 1102 30-31 0.31438055541088517 1102 32-33 -0.04002395170999762 1102 34-35 0.04341577179726386 1102 36-37 0.5235012310556328 1102 38-39 0.3516223946076309 1102 40-41 0.3611554388217755 1102 42-43 0.6290984212025634 1102 44-45 0.4467117799325706 1102 46-47 0.20470443243633696 1102 48-49 0.1678495121260326 1102 50-51 -0.48370335772955286 1102 52-53 0.47816856731788704 1102 54-55 0.8687309598375066 1102 56-57 0.5341103831889278 1102 58-59 0.3029228956214389 1102 60-61 -0.13195236954301848 1102 62-63 0.031424555034565316 1102 64-65 0.14408200360523082 1102 66-67 0.40902139576799357 1102 68-69 0.6430454860300578 1102 70-71 0.6118980844510133 1102 72-73 1.1178380749972163 1102 74-75 0.9491113979319614 1102 76-77 1.16735067424478 1102 78-79 1.2081861695318459 1102 80-81 1.21721212894699 1102 82-83 0.9970043594385132 1102 84-85 1.1315552694543243 1102 86-87 0.7881983230162675 1102 88-89 0.9934384682964357 1102 90-91 1.5379806372524598 1102 92-93 1.6936835299017012 1102 94-95 1.8282860292914123 1102 96-97 1.9039756767437161 1102 98-99 2.0663644496007443 1102 100-101 2.404669100360131 1103 1 0.27059083221666214 1103 2 0.24652285451675482 1103 3 0.21175335198897471 1103 4 -0.18148558376812218 1103 5 -0.16635577306311689 1103 6 -0.031184374007821702 1103 7 0.09732134716657725 1103 8 0.14029029033650886 1103 9 0.1652772733286767 1103 10-11 -0.038377401629695385 1103 12-13 0.05160606749252139 1103 14-15 0.22934914744300272 1103 16-17 0.40059436981361074 1103 18-19 0.09049418068357795 1103 20-21 -0.1289407962659581 1103 22-23 -0.035265795856922466 1103 24-25 0.09494431067595599 1103 26-27 -0.5044562905500172 1103 28-29 -0.4969447451101985 1103 30-31 -0.6576890416587062 1103 32-33 -0.24942566111170805 1103 34-35 0.055106858488343846 1103 36-37 0.4307051382595404 1103 38-39 0.6798580478432825 1103 40-41 0.6616438393101802 1103 42-43 0.6018395689437099 1103 44-45 0.5583418165626028 1103 46-47 0.4856873384192397 1103 48-49 0.9564942007707202 1103 50-51 0.6608326618064737 1103 52-53 0.5538401179894308 1103 54-55 0.61952461063116 1103 56-57 0.403615877694417 1103 58-59 0.99645158915013 1103 60-61 1.2597142971236472 1103 62-63 0.8668641154741259 1103 64-65 0.536237070760297 1103 66-67 0.5420494787960735 1103 68-69 0.6535766215611964 1103 70-71 0.8071361796891097 1103 72-73 0.6744314815906236 1103 74-75 0.5957841696047304 1103 76-77 0.6036754605695691 1103 78-79 1.2131312244769035 1103 80-81 0.8646785514134123 1103 82-83 0.8777430651772171 1103 84-85 0.9700778579769143 1103 86-87 1.092899177717122 1103 88-89 1.4869366367945993 1103 90-91 1.6354470597188815 1103 92-93 1.6050083162264919 1103 94-95 1.327736578741959 1103 96-97 1.0110880088560457 1103 98-99 1.4949358781721749 1103 100-101 1.7601941308851643 1104 1 0.09716481072434391 1104 2 0.08508760468371435 1104 3 0.011461930703077883 1104 4 0.08303994595078024 1104 5 -0.2390289562335397 1104 6 0.013712717850594913 1104 7 0.19257967999396897 1104 8 0.2027637285005568 1104 9 -0.06734369686135011 1104 10-11 0.07066272950988406 1104 12-13 0.08065712693032623 1104 14-15 0.1269115298120127 1104 16-17 -0.007896310776516202 1104 18-19 -0.0887754447186424 1104 20-21 0.2956691242003089 1104 22-23 0.024430033175924848 1104 24-25 0.2068379419065991 1104 26-27 0.48643679426572106 1104 28-29 0.06442068503976373 1104 30-31 -0.3870467005743805 1104 32-33 0.19171331041400208 1104 34-35 -0.1509826321315373 1104 36-37 -0.0811642078639494 1104 38-39 -0.13114310569930865 1104 40-41 -0.14522884601554864 1104 42-43 -0.10283834982260487 1104 44-45 -0.30931952761807224 1104 46-47 0.6691765011901722 1104 48-49 0.8709802671904896 1104 50-51 0.5461462432526432 1104 52-53 0.32560564527981484 1104 54-55 0.49482058537464724 1104 56-57 0.36905513457014294 1104 58-59 0.6971831780032751 1104 60-61 0.6961783855214385 1104 62-63 0.5578057705041779 1104 64-65 0.6865467058765624 1104 66-67 0.7687205977323899 1104 68-69 0.6759644547225072 1104 70-71 0.26116143620055254 1104 72-73 0.45844580980384464 1104 74-75 0.5808640068945152 1104 76-77 0.4322984949937059 1104 78-79 -0.07523924483279032 1104 80-81 -0.07052574705055292 1104 82-83 0.3403591783458175 1104 84-85 0.7644588913082231 1104 86-87 1.1002302444404535 1104 88-89 1.2498380999723082 1104 90-91 1.0068725955090585 1104 92-93 0.9004821793799103 1104 94-95 1.3169600624405824 1104 96-97 2.01307635200461 1104 98-99 1.7694031236338965 1104 100-101 1.1800988118395672 1105 1 0.13306253137177748 1105 2 0.09444377781456126 1105 3 0.15294281579150493 1105 4 0.18562469288989547 1105 5 0.22367128530564884 1105 6 0.20425104484468193 1105 7 0.16712089435306154 1105 8 0.17430652535777114 1105 9 0.27972312898056373 1105 10-11 0.4037508219055681 1105 12-13 0.20542461075830687 1105 14-15 0.2755830642799282 1105 16-17 0.3424326822549375 1105 18-19 0.19407527295813765 1105 20-21 0.20761837320778653 1105 22-23 0.40602030497692 1105 24-25 0.29948349008950714 1105 26-27 0.008216965841832291 1105 28-29 -0.013635340946521524 1105 30-31 -0.3047567980229431 1105 32-33 -0.3267352861600301 1105 34-35 -0.33616267599727223 1105 36-37 -0.7249633138913225 1105 38-39 -0.2173753720032039 1105 40-41 -0.40997800618101365 1105 42-43 -0.3087435665137903 1105 44-45 0.23318581678348949 1105 46-47 0.035455409544091765 1105 48-49 -0.18357427346860788 1105 50-51 -0.16132759000202412 1105 52-53 0.26126460305210486 1105 54-55 -0.030152345578457584 1105 56-57 0.0158943027869185 1105 58-59 0.22182295145617204 1105 60-61 0.02604595541510335 1105 62-63 0.34913812287375734 1105 64-65 0.1719016574299097 1105 66-67 0.20050659956475414 1105 68-69 0.4606753017754528 1105 70-71 0.6386150023739603 1105 72-73 0.7251245071580179 1105 74-75 0.5023637709079516 1105 76-77 0.72592130517722 1105 78-79 0.91823642078813 1105 80-81 1.3150485331904278 1105 82-83 1.6040255766356104 1105 84-85 1.4408796692309878 1105 86-87 1.665473588231233 1105 88-89 1.7057919021322796 1105 90-91 1.9702391520486628 1105 92-93 2.1091637085728365 1105 94-95 2.338587536829099 1105 96-97 2.46526825072473 1105 98-99 2.2435001279927036 1105 100-101 1.933326275122834 1106 1 0.18890585053168252 1106 2 0.24579025378415054 1106 3 0.15168009191571485 1106 4 0.18041917813664554 1106 5 0.002874996167655297 1106 6 0.11423687141342498 1106 7 0.3888964387416678 1106 8 0.10439285443907664 1106 9 0.02351903157043722 1106 10-11 0.12023065697835733 1106 12-13 0.28567200155845995 1106 14-15 0.2628656309594817 1106 16-17 -0.07797705875780991 1106 18-19 -0.16426772407832857 1106 20-21 -0.15000306732823532 1106 22-23 -0.1299544405455677 1106 24-25 -0.44498242925078557 1106 26-27 -0.24841233450606381 1106 28-29 -0.11947221763767857 1106 30-31 -0.17728611125577487 1106 32-33 -0.1417333534194043 1106 34-35 -0.08884918546770137 1106 36-37 -0.7132509056964977 1106 38-39 -0.7032921353069028 1106 40-41 -0.33798986032351763 1106 42-43 -0.4463289292247836 1106 44-45 0.04863485685564228 1106 46-47 0.10235400508590686 1106 48-49 0.6165674608439815 1106 50-51 0.5344590354328389 1106 52-53 0.3771001912495038 1106 54-55 0.2730044641110112 1106 56-57 -0.22550500142645902 1106 58-59 -0.2103249676264234 1106 60-61 -0.39413185672250606 1106 62-63 -0.6935754449654361 1106 64-65 -0.31083252630930147 1106 66-67 -0.23926920252260686 1106 68-69 -0.46272374473916855 1106 70-71 -1.056050633497705 1106 72-73 -0.48087987372073115 1106 74-75 -0.2293074054868427 1106 76-77 -0.34632453943043373 1106 78-79 -0.4071984458527673 1106 80-81 -1.2479588112239526 1106 82-83 -0.8967990593649056 1106 84-85 -0.9641712262721711 1106 86-87 -0.20069056587262324 1106 88-89 0.37100257086053645 1106 90-91 0.44075841503023483 1106 92-93 -0.05011988890171537 1106 94-95 0.29110654211192255 1106 96-97 0.607608155376191 1106 98-99 0.911236244472537 1106 100-101 0.8241135448045753 1107 1 0.1300884774285933 1107 2 0.24691531919493315 1107 3 0.2196026455525555 1107 4 0.2506180269069205 1107 5 -0.20128512942104493 1107 6 0.18427873431242858 1107 7 0.19229779928588897 1107 8 0.04688369692991756 1107 9 -0.08236793860224623 1107 10-11 0.02637927026983533 1107 12-13 0.19387451333240335 1107 14-15 0.29861916314158776 1107 16-17 0.2710810260145564 1107 18-19 0.09696984787353102 1107 20-21 0.06258196668537863 1107 22-23 0.060299353279660295 1107 24-25 0.1967888946633991 1107 26-27 0.20511984759755109 1107 28-29 0.2198919895836724 1107 30-31 0.30757783432245134 1107 32-33 0.3023796764079094 1107 34-35 0.14046792599226876 1107 36-37 -0.15308607053167123 1107 38-39 -0.08505560992752237 1107 40-41 -0.15919603510112523 1107 42-43 0.17660409013680578 1107 44-45 0.3295349091842752 1107 46-47 -0.12381030679268434 1107 48-49 -0.32313688243178973 1107 50-51 -0.5222521105640183 1107 52-53 -0.08725093381590199 1107 54-55 0.027687875937282058 1107 56-57 0.09788589339300913 1107 58-59 -0.39974150347152815 1107 60-61 -0.7657959069579867 1107 62-63 -0.6246978939450294 1107 64-65 -0.3738885179367202 1107 66-67 -0.3425659058193098 1107 68-69 -1.4700497520651794 1107 70-71 -1.4148025958210937 1107 72-73 -1.7627741699007444 1107 74-75 -1.7521357676009224 1107 76-77 -1.813318259995583 1107 78-79 -1.8647730141416226 1107 80-81 -1.8556903653840777 1107 82-83 -1.5879162754821223 1107 84-85 -1.534827950500322 1107 86-87 -1.272289205328402 1107 88-89 -1.368636361635538 1107 90-91 -1.2864524693235033 1107 92-93 -0.7150466288284569 1107 94-95 -0.5839588686677715 1107 96-97 -0.6600265908299825 1107 98-99 -0.7244518769298693 1107 100-101 0.06356932711750218 1108 1 0.15520621683204894 1108 2 0.21905032704422922 1108 3 -0.09593895570333189 1108 4 0.20312980084726462 1108 5 -0.11690522361256939 1108 6 0.062222219398769596 1108 7 0.025892775738007856 1108 8 0.20072985077607086 1108 9 0.2476948557462606 1108 10-11 -0.10705872031100938 1108 12-13 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2.1750074227754617 2316 98-99 2.0761047093410028 2316 100-101 2.27909189978293 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 16.0 6 39.0 7 84.0 8 146.0 9 295.0 10 484.0 11 775.0 12 1070.0 13 1592.0 14 1881.0 15 2568.0 16 3398.0 17 4317.0 18 4343.0 19 3312.0 20 3001.0 21 3066.0 22 3354.0 23 3508.0 24 3626.0 25 3674.0 26 3467.0 27 3607.0 28 4237.0 29 5088.0 30 5865.0 31 6957.0 32 8490.0 33 10466.0 34 15309.0 35 24796.0 36 22711.0 37 29559.0 38 15771.0 39 612.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.69623651911519 23.300527572224787 16.137357374347978 15.865878534312047 2 15.285451197053407 26.38630350503591 38.17168272500285 20.156562572907823 3 20.0715689583292 31.549899743900262 33.827499950368264 14.551031347402274 4 10.51602368438032 23.395025883076983 37.498945320448875 28.590005112093824 5 8.12563675670303 43.952488631563256 34.3503217950948 13.571552816638919 6 21.687039987294593 41.55313689581069 17.207390974197104 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34.317258014085134 36.88340306418713 14.751177483634342 30-31 15.257904898034136 32.846706271806404 36.63500240707929 15.260386423080169 32-33 15.1320915782003 33.29387708510142 37.68394304403714 13.890088292661137 34-35 14.985494226230509 32.83069813844638 37.522676904673865 14.66113073064925 36-37 14.907054248753651 32.548011424969665 37.81630217109165 14.728632155185034 38-39 14.176270883587957 33.07200929152149 37.68525963647915 15.066460188411407 40-41 13.89341811043359 33.28593161744086 37.927080774012936 14.893569498112619 42-43 14.16184397303924 33.1616851467654 36.46725697098442 16.20921390921093 44-45 14.130011291303216 32.37377936049037 37.040437755140026 16.455771593066395 46-47 16.183265587699577 32.81196492116195 35.90295251849977 15.101816972638705 48-49 15.189662959268249 32.121356500851164 36.36550878708019 16.3234717528004 50-51 12.908396984450764 33.015201822432 37.641757118254596 16.434644074862646 52-53 14.303014060320912 33.71970678300056 35.70219714227576 16.275082014402773 54-55 13.97396383921703 32.416657981329 35.81535468437483 17.794023495079138 56-57 15.93982798068381 32.44271399431234 34.08424281226271 17.533215212741144 58-59 15.872826804440937 32.84720257681561 34.80934443071334 16.470626188030117 60-61 15.16757738635856 34.22717865491416 34.4832720396647 16.12197191906258 62-63 13.749633975055712 34.777829062628726 34.25373097290671 17.21880598940885 64-65 13.667743648536646 35.49945654601492 33.36683392145477 17.46596588399367 66-67 14.682935544868455 33.71300666537627 32.773501282948445 18.830556506806822 68-69 14.052166748970784 33.36642041605352 33.58057655895994 19.000836276015754 70-71 15.853222756577281 32.98418275935659 32.51467822064728 18.647916263418846 72-73 15.396373995602739 31.32404250356099 34.48227942964628 18.79730407118999 74-75 14.444460987944751 33.13580393966916 32.84720257681561 19.572532495570478 76-77 14.68095032483163 31.39873640744656 33.85544620301852 20.064867064703286 78-79 14.756140533726406 31.117827772235707 34.261920005558615 19.864111688479273 80-81 15.672815885730735 30.98854031733742 33.32961104576428 20.009032751167556 82-83 14.983596956627473 30.43124370306769 34.4796438479902 20.10551549231464 84-85 14.829759777645425 30.611226920786184 33.731884057971016 20.82712924359738 86-87 15.025697148373423 31.19641257385914 33.159620114005506 20.618270163761935 88-89 15.072286824590922 30.556754959327804 32.138975328677994 22.23198288740328 90-91 15.763325532860012 28.871098625942075 33.39677938888296 21.968796452314955 92-93 16.372357796207236 29.710058613621587 33.12215555191599 20.79542803825519 94-95 15.87530832948697 28.969075234876346 33.51622172922591 21.639394706410773 96-97 14.813960067298959 29.33609278918452 33.78348197668359 22.06646516683293 98-99 15.537076465712769 29.259661817766723 32.055596087131306 23.1476656293892 100-101 15.416970653484805 28.21618053591015 33.23357602648284 23.133272784122212 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4334.0 1 2606.0 2 1074.5 3 1283.0 4 976.0 5 870.0 6 1715.0 7 3223.5 8 4327.0 9 4441.5 10 3380.5 11 2132.5 12 1751.5 13 1604.0 14 1437.0 15 1299.0 16 1211.5 17 1133.5 18 1145.5 19 1139.0 20 1125.0 21 1184.5 22 1266.0 23 1349.5 24 1455.5 25 1614.0 26 1922.0 27 2351.5 28 2588.0 29 2882.0 30 3397.0 31 3870.5 32 4622.5 33 5300.0 34 5601.0 35 6015.0 36 6339.5 37 6500.5 38 6234.5 39 5793.0 40 5650.0 41 5466.5 42 5195.0 43 5481.0 44 5740.5 45 5305.5 46 4941.5 47 6765.0 48 10761.5 49 10297.0 50 6381.0 51 4583.5 52 3906.5 53 3215.0 54 2142.5 55 1209.5 56 810.0 57 614.0 58 516.0 59 463.0 60 371.0 61 299.0 62 230.0 63 162.0 64 126.5 65 98.0 66 74.0 67 57.0 68 50.0 69 36.5 70 20.0 71 21.5 72 29.5 73 36.5 74 37.0 75 30.5 76 19.5 77 7.0 78 5.5 79 3.5 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.017866980331432483 3 0.0024815250460322896 4 0.0029778300552387475 5 0.1359875725225695 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 2.4815250460322896E-4 24-25 0.0 26-27 0.006451965119683953 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.008685337661113013 36-37 2.4815250460322896E-4 38-39 0.007444575138096868 40-41 0.00967794767952593 42-43 0.0054593551012710375 44-45 0.0032259825598419767 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.4815250460322896E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 9.926100184129158E-4 84-85 0.004466745082858121 86-87 0.003722287569048434 88-89 0.0 90-91 0.0027296775506355187 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 201489.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.49032949689561 #Duplication Level Percentage of deduplicated Percentage of total 1 78.38800935748871 28.604042900605 2 10.198030575050323 7.442589918060043 3 3.6641096784723355 4.011137084406593 4 1.8266144388226973 2.666150509457092 5 1.1384037865186878 2.0770364635290264 6 0.7847777596431097 1.7182079418727574 7 0.5644415428975573 1.4417660517447604 8 0.44203253359447253 1.2903930239367907 9 0.3182634241880202 1.0452183493888003 >10 2.3434524781023884 15.918983170297137 >50 0.208095315815244 5.263810927643693 >100 0.08840650671889451 6.540803716331908 >500 0.0176813013437789 4.676682101752453 >1k 0.014961101137043686 11.656715751232078 >5k 0.0027202002067352156 5.646462089741871 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5978 2.9669113450362055 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5399 2.6795507447056663 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4902 2.432887155130057 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3784 1.8780181548372368 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2885 1.4318399515606313 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1981 0.9831802232379931 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 1926 0.9558834477316379 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1781 0.8839192213967015 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1527 0.7578577490582612 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1284 0.637255631821092 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 1220 0.6054921112318786 TruSeq Adapter, Index 10 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1134 0.5628098804401233 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1063 0.5275722247864648 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 913 0.4531264734054961 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 851 0.4223555628346957 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 839 0.41639990272421823 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 790 0.39208095727310177 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 786 0.39009573723627594 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 783 0.38860682220865655 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 741 0.3677620118219853 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 687 0.3409615413248366 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 679 0.3369911012511849 No Hit ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 656 0.32557608603943644 RNA PCR Primer, Index 10 (95% over 23bp) ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 606 0.3007608355791135 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 557 0.2764418901279971 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 535 0.265523179925455 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 496 0.24616728456640313 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 486 0.24120423447433853 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 473 0.2347522693546546 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 456 0.22631508419814478 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 450 0.2233372541429061 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 421 0.20894440887591878 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 377 0.18710698847083465 No Hit CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 371 0.1841291584155959 TruSeq Adapter, Index 10 (96% over 26bp) GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 329 0.16328434802892466 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 313 0.15534346788162134 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 296 0.14690628272511155 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 278 0.13797279255939532 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 265 0.13152082743971136 No Hit TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC 262 0.13003191241209197 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 259 0.1285429973844726 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 256 0.12705408235685323 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 253 0.12556516732923384 TruSeq Adapter, Index 10 (96% over 28bp) TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 247 0.12258733727399511 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 238 0.11812059219113698 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 238 0.11812059219113698 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 229 0.11365384710827886 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 221 0.10968340703462721 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 218 0.10819449200700783 No Hit CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 211 0.10472035694256263 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 204 0.10124622187811741 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 203 0.10074991686891095 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.4815250460322896E-4 0.0 12-13 0.0 0.0 0.0 4.963050092064579E-4 0.0 14-15 0.0 0.0 0.0 4.963050092064579E-4 0.0 16-17 0.0 0.0 0.0 4.963050092064579E-4 0.0 18-19 0.0 0.0 0.0 4.963050092064579E-4 0.0 20-21 0.0 0.0 0.0 0.0012407625230161448 0.0 22-23 0.0 0.0 0.0 0.007444575138096869 0.0 24-25 0.0 0.0 0.0 0.020348505377464778 0.0 26-27 0.0 0.0 0.0 0.0436748408101683 0.0 28-29 0.0 0.0 0.0 0.10397589942875293 0.0 30-31 0.0 0.0 0.0 0.24095608196973534 0.0 32-33 0.0 0.0 0.0 0.45287832090089286 0.0 34-35 0.0 0.0 0.0 0.7633171041595322 0.0 36-37 0.0 0.0 0.0 1.166068619130573 0.0 38-39 0.0 0.0 0.0 1.6683292884475083 0.0 40-41 0.0 0.0 0.0 2.1150037967333204 0.0 42-43 0.0 0.0 0.0 2.5291703269161094 0.0 44-45 0.0 0.0 0.0 2.9597149224027115 0.0 46-47 0.0 0.0 0.0 3.459494066673615 0.0 48-49 0.0 0.0 0.0 3.8994684573351397 0.0 50-51 0.0 0.0 0.0 4.310905309967294 0.0 52-53 0.0 0.0 0.0 4.693060167056267 0.0 54-55 0.0 0.0 0.0 5.111941594826517 0.0 56-57 0.0 0.0 0.0 5.593109301252177 0.0 58-59 0.0 0.0 0.0 6.059636009906248 0.0 60-61 0.0 0.0 0.0 6.512514330807141 0.0 62-63 0.0 0.0 0.0 6.937351418687869 0.0 64-65 0.0 0.0 0.0 7.361444049054787 0.0 66-67 0.0 0.0 0.0 7.844845128021877 0.0 68-69 0.0 0.0 0.0 8.383584215515487 0.0 70-71 0.0 0.0 0.0 8.92803081061497 0.0 72-73 0.0 0.0 0.0 9.458580865456675 0.0 74-75 0.0 0.0 0.0 9.990123530316792 0.0 76-77 0.0 0.0 0.0 10.472780151770072 0.0 78-79 0.0 0.0 0.0 11.087702058176873 0.0 80-81 0.0 0.0 0.0 11.700142439537643 0.0 82-83 0.0 0.0 0.0 12.230940646883948 0.0 84-85 0.0 0.0 0.0 12.805661847545027 0.0 86-87 0.0 0.0 0.0 13.388075775848804 0.0 88-89 0.0 0.0 0.0 13.993319734576081 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGGACT 25 0.0049974048 57.05143 4 AGCGTAG 20 5.3864165E-4 47.49566 32-33 ATTCGGG 20 5.3864165E-4 47.49566 66-67 GGTGTAC 75 1.356293E-7 44.32928 8 GACACCT 65 2.580322E-6 43.842144 7 GTATAGT 45 9.5518946E-4 42.27082 1 GTGTACG 80 2.251818E-7 41.5587 9 GTTAGAG 95 1.895387E-8 40.04604 1 TAGTTTA 30 8.285623E-5 39.579716 16-17 GGCTAGG 50 0.0016035611 38.04374 1 TAGGACA 50 0.0016055207 38.034286 4 TGGCGTA 25 0.0016152958 37.996525 42-43 GGCGTAT 25 0.0016152958 37.996525 42-43 CGTAGAA 25 0.0016152958 37.996525 34-35 TTCGGGT 25 0.0016152958 37.996525 66-67 GTAAGCG 25 0.0016152958 37.996525 94-95 GGACACC 50 0.0016133782 37.996525 6 ATGGGTA 135 3.0377123E-10 35.208183 5 AGGGTGT 100 1.2930777E-6 33.24696 6 CATGGGT 190 0.0 32.529324 4 >>END_MODULE