##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938042_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 200039 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.34495773324202 33.0 31.0 34.0 31.0 34.0 2 32.54010467958748 34.0 31.0 34.0 31.0 34.0 3 32.43653487569924 34.0 31.0 34.0 30.0 34.0 4 36.02444023415434 37.0 35.0 37.0 35.0 37.0 5 36.02387534430786 37.0 35.0 37.0 35.0 37.0 6 36.15406995635851 37.0 36.0 37.0 35.0 37.0 7 36.11601737661156 37.0 36.0 37.0 35.0 37.0 8 36.15910897375012 37.0 36.0 37.0 35.0 37.0 9 37.75204335154645 39.0 38.0 39.0 35.0 39.0 10-11 37.76125405545918 39.0 38.0 39.0 35.0 39.0 12-13 37.718357420303036 39.0 38.0 39.0 35.0 39.0 14-15 38.74879148566029 40.0 38.0 41.0 34.5 41.0 16-17 38.712776008678304 40.0 38.0 41.0 35.0 41.0 18-19 38.79917416103859 40.0 38.0 41.0 35.0 41.0 20-21 38.88787686401152 40.0 38.0 41.0 35.0 41.0 22-23 38.80184114097751 40.0 38.0 41.0 35.0 41.0 24-25 38.653215123051005 40.0 38.0 41.0 35.0 41.0 26-27 38.334852203820255 40.0 37.0 41.0 34.5 41.0 28-29 38.12966221586791 40.0 37.0 41.0 34.0 41.0 30-31 37.70366028624418 39.5 35.5 41.0 33.5 41.0 32-33 37.16455791120731 39.0 35.0 41.0 33.0 41.0 34-35 36.76959492898885 38.5 35.0 41.0 33.0 41.0 36-37 36.45291418173456 38.0 35.0 41.0 32.5 41.0 38-39 36.13390638825429 38.0 35.0 41.0 31.0 41.0 40-41 35.95837311724214 38.0 35.0 41.0 31.0 41.0 42-43 35.57066871959968 38.0 35.0 41.0 30.5 41.0 44-45 35.08841525902449 38.0 35.0 41.0 27.5 41.0 46-47 34.41496658151661 38.0 35.0 40.0 23.5 41.0 48-49 33.76283374741925 38.0 34.0 40.0 19.0 41.0 50-51 33.302346042521705 37.0 33.5 40.0 10.0 41.0 52-53 32.86603362344343 37.0 33.0 40.0 3.5 41.0 54-55 32.53441328940856 36.5 33.0 40.0 2.0 41.0 56-57 32.167609816085864 36.0 33.0 40.0 2.0 41.0 58-59 31.709681612085646 35.5 32.5 40.0 2.0 41.0 60-61 31.137023280460312 35.0 31.0 39.5 2.0 41.0 62-63 30.664595403896243 35.0 31.0 39.0 2.0 41.0 64-65 30.154654842305753 35.0 30.5 39.0 2.0 40.5 66-67 29.475629752198323 35.0 29.0 38.5 2.0 40.0 68-69 28.823759366923447 35.0 27.5 37.5 2.0 40.0 70-71 28.200255950089733 35.0 26.0 37.0 2.0 39.0 72-73 27.733254515369502 34.0 26.0 36.0 2.0 39.0 74-75 27.259411914676637 34.0 24.5 36.0 2.0 38.5 76-77 26.040842035803017 32.0 23.0 35.0 2.0 36.5 78-79 26.691040247151804 34.0 24.0 35.0 2.0 37.0 80-81 26.52028604422138 34.0 24.0 35.0 2.0 37.0 82-83 26.14190482855843 33.5 21.5 35.0 2.0 36.0 84-85 25.71451067041927 33.0 19.0 35.0 2.0 36.0 86-87 25.31463114692635 33.0 14.5 35.0 2.0 35.0 88-89 24.77500137473193 33.0 4.5 35.0 2.0 35.0 90-91 24.347067321872235 33.0 2.0 35.0 2.0 35.0 92-93 23.86617359614875 32.0 2.0 35.0 2.0 35.0 94-95 23.271457065872156 31.5 2.0 35.0 2.0 35.0 96-97 22.60778148261089 31.0 2.0 34.0 2.0 35.0 98-99 21.88797684451532 31.0 2.0 34.0 2.0 35.0 100-101 19.12558551082539 26.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.0769553821280553 1101 2 0.018278626870497305 1101 3 0.030633054952154737 1101 4 0.17680978022977456 1101 5 -0.1511647545112993 1101 6 0.024424732392908766 1101 7 0.043966631568785886 1101 8 0.04009076290317637 1101 9 -0.20961814564953585 1101 10-11 -0.09132060547511145 1101 12-13 0.050323006913608026 1101 14-15 0.3089923641422061 1101 16-17 0.1349636443300284 1101 18-19 0.14708113686300806 1101 20-21 0.039440681033632075 1101 22-23 0.15248599711379995 1101 24-25 -0.11236535879230303 1101 26-27 0.019587300345349945 1101 28-29 -0.1568003938351481 1101 30-31 -0.07174227384740561 1101 32-33 -0.672640463649131 1101 34-35 -0.7183607356969759 1101 36-37 -0.5636994603590111 1101 38-39 -0.49127111607080565 1101 40-41 -1.1811745231188624 1101 42-43 -1.010026613118022 1101 44-45 -0.6092969278936877 1101 46-47 -0.65800287707048 1101 48-49 -0.2777769998293067 1101 50-51 0.02707044585265095 1101 52-53 -0.19872247865739467 1101 54-55 -1.3429352245656148 1101 56-57 -1.4805334677926325 1101 58-59 -1.4088855656810715 1101 60-61 -1.3047638731790414 1101 62-63 -1.9309139673096603 1101 64-65 -2.437095124169282 1101 66-67 -1.9225781976834924 1101 68-69 -2.1995090032368125 1101 70-71 -2.4481547285197784 1101 72-73 -2.7316408724343653 1101 74-75 -2.445777040829615 1101 76-77 -2.0235433255240345 1101 78-79 -2.2584218333274677 1101 80-81 -2.421476356592457 1101 82-83 -2.4199510635021433 1101 84-85 -1.9031163888506875 1101 86-87 -1.8871807354591823 1101 88-89 -2.023614554125256 1101 90-91 -1.9693674069975486 1101 92-93 -2.336661509423429 1101 94-95 -1.928897224632145 1101 96-97 -1.7106034203132587 1101 98-99 -1.6258914877787483 1101 100-101 -1.1173931277043074 1102 1 -0.05782722656759631 1102 2 -0.032325237863801703 1102 3 0.15007749939660187 1102 4 -0.02391485745138766 1102 5 -0.0074449477480129644 1102 6 0.03106724447020781 1102 7 -0.019439165532659786 1102 8 0.014003806381431616 1102 9 0.011637892997804045 1102 10-11 -0.07078920450893378 1102 12-13 0.10110803106820043 1102 14-15 -0.06794000300755698 1102 16-17 0.06926316123823995 1102 18-19 0.15547485666977678 1102 20-21 0.00514116412541199 1102 22-23 0.18068648020557987 1102 24-25 0.15025541415455024 1102 26-27 0.16052932933085629 1102 28-29 -0.2581892827240395 1102 30-31 -0.29016014824353675 1102 32-33 0.016308811713187765 1102 34-35 -0.24444769221871354 1102 36-37 0.027967206307657477 1102 38-39 0.22098733803547077 1102 40-41 0.0069897280888611135 1102 42-43 0.03689367673705135 1102 44-45 0.2775992073720204 1102 46-47 0.30437634998266105 1102 48-49 -0.12777699982930102 1102 50-51 0.03280716082849011 1102 52-53 0.22410360829913145 1102 54-55 0.1457725049029861 1102 56-57 0.6199496239948061 1102 58-59 0.570522646879315 1102 60-61 0.3637747741639572 1102 62-63 0.715040138970533 1102 64-65 0.3745594652027009 1102 66-67 0.5914918506256868 1102 68-69 0.41860693879217337 1102 70-71 1.0092728077121045 1102 72-73 1.2228881130728801 1102 74-75 1.3254186113443005 1102 76-77 1.2319518435580932 1102 78-79 1.5127134323730154 1102 80-81 1.7709149477553723 1102 82-83 1.4890465210389188 1102 84-85 1.654311147381197 1102 86-87 1.4341356896615913 1102 88-89 1.437617329932717 1102 90-91 1.893676071263318 1102 92-93 1.7586887321224651 1102 94-95 0.8188684758509446 1102 96-97 1.4357130048075142 1102 98-99 1.9034563383082101 1102 100-101 2.2172083215710536 1103 1 -0.15156810780483454 1103 2 0.01768205714641624 1103 3 0.055278841678479296 1103 4 0.1716643663595292 1103 5 -0.261530153467298 1103 6 0.13870512016323033 1103 7 0.0652194279893692 1103 8 0.13539277632599322 1103 9 0.21144822273226538 1103 10-11 0.1475869262176488 1103 12-13 0.07015895023948104 1103 14-15 0.1664122000855386 1103 16-17 -0.007225020845218921 1103 18-19 0.3696016439472771 1103 20-21 0.22424679587329877 1103 22-23 0.3238692931615219 1103 24-25 0.5176868410585556 1103 26-27 0.3762427813296938 1103 28-29 0.42847179110146527 1103 30-31 -0.0291993953984786 1103 32-33 0.22875774664169057 1103 34-35 0.6865050361151006 1103 36-37 0.5715913673814796 1103 38-39 0.14174528959660648 1103 40-41 0.5146494888125304 1103 42-43 0.8211255121616219 1103 44-45 0.7802071735231664 1103 46-47 1.2825825069712877 1103 48-49 1.965343805539824 1103 50-51 1.59213383138583 1103 52-53 2.0356734944969688 1103 54-55 2.2064122772986607 1103 56-57 2.229776002751734 1103 58-59 1.5373485879356288 1103 60-61 1.2357655824510871 1103 62-63 1.4037802907067451 1103 64-65 1.0391166580827687 1103 66-67 0.42095274937094374 1103 68-69 0.776609564995848 1103 70-71 0.7420018710327945 1103 72-73 0.39816897096607917 1103 74-75 0.19784712024421225 1103 76-77 0.11262371399871185 1103 78-79 0.3144715298343499 1103 80-81 0.8983223011256669 1103 82-83 1.0743069529035232 1103 84-85 0.9667381972790672 1103 86-87 1.2104889140561035 1103 88-89 1.2299648642192658 1103 90-91 0.9579815862910408 1103 92-93 0.6702549708152006 1103 94-95 0.3349357358451108 1103 96-97 0.5082071687993412 1103 98-99 0.7392091833621954 1103 100-101 0.3313205188281323 1104 1 -0.06148682892722235 1104 2 0.17919431642940253 1104 3 0.36929860492423927 1104 4 0.2650844870974467 1104 5 0.26145389428825894 1104 6 0.23199033819972215 1104 7 0.06825473877146493 1104 8 0.06119629054136766 1104 9 0.2332350090126667 1104 10-11 0.39646554751204377 1104 12-13 0.23128336425587293 1104 14-15 0.28291754560508053 1104 16-17 0.16990222612790262 1104 18-19 0.4295490318937212 1104 20-21 0.4032458178287115 1104 22-23 0.1745825911953105 1104 24-25 0.13464748319541542 1104 26-27 0.30882515629174634 1104 28-29 0.2264212700397792 1104 30-31 -0.1987383654163608 1104 32-33 -0.04001902311311767 1104 34-35 0.29206639998141526 1104 36-37 0.44756519625740765 1104 38-39 0.5942258130190865 1104 40-41 0.9191046505271387 1104 42-43 1.1746886297630468 1104 44-45 0.9705886109114488 1104 46-47 0.6290295070724596 1104 48-49 0.9607908393666733 1104 50-51 0.8820397367515866 1104 52-53 1.1589408379255204 1104 54-55 1.4202836475728517 1104 56-57 1.5531209152336132 1104 58-59 1.324168594006256 1104 60-61 1.7099485779655765 1104 62-63 1.2251859768556095 1104 64-65 1.6860762884493674 1104 66-67 1.8264028185085799 1104 68-69 2.093748953212099 1104 70-71 2.145647616538845 1104 72-73 2.181717586527107 1104 74-75 2.6039717028889804 1104 76-77 2.293932944716058 1104 78-79 2.173599383869629 1104 80-81 1.8832975127542788 1104 82-83 2.3593900866932778 1104 84-85 2.741062840630047 1104 86-87 2.356942659292354 1104 88-89 2.4141576401796065 1104 90-91 2.2036225427511944 1104 92-93 2.2278415614866773 1104 94-95 2.274229520203143 1104 96-97 2.6769873111216924 1104 98-99 2.6826512257890904 1104 100-101 2.965228312831705 1105 1 0.07793577428491716 1105 2 -0.08400895482884607 1105 3 0.08390102880836992 1105 4 0.02190781944545961 1105 5 0.16092674875667967 1105 6 0.040764601673956236 1105 7 0.18612349431388964 1105 8 0.0018554687855285579 1105 9 0.47796355369686694 1105 10-11 -0.18854282769733288 1105 12-13 0.12663019645609097 1105 14-15 0.14657406348860746 1105 16-17 -0.2965723033823906 1105 18-19 -0.08690579124156983 1105 20-21 0.12832956992252065 1105 22-23 0.16049253306150746 1105 24-25 0.07603333401815604 1105 26-27 0.15488141799241362 1105 28-29 -0.026571635665213478 1105 30-31 -0.1410233195990358 1105 32-33 -0.22116987541383537 1105 34-35 0.09144318587165401 1105 36-37 -0.19507200937861313 1105 38-39 -0.3793430114956493 1105 40-41 -0.05257975187703323 1105 42-43 -0.2659089660592002 1105 44-45 -0.8194276468479273 1105 46-47 -0.8591466679201503 1105 48-49 -1.2503260194371464 1105 50-51 -1.4516877240819923 1105 52-53 -1.1065656159122952 1105 54-55 -0.9873796690100605 1105 56-57 -0.7970367357664898 1105 58-59 -1.0742450428052557 1105 60-61 -0.7799272718718484 1105 62-63 -0.45967213724430067 1105 64-65 -0.3607879346267957 1105 66-67 -0.3219246029122509 1105 68-69 -0.5897050816681855 1105 70-71 -0.7471743363629209 1105 72-73 -0.5610526371402464 1105 74-75 -0.5639142957315748 1105 76-77 -0.08694201833448645 1105 78-79 -0.09012117973269795 1105 80-81 -0.44647635659245566 1105 82-83 -0.5766504099073693 1105 84-85 -0.19037129081147341 1105 86-87 -0.23407616029578548 1105 88-89 -0.22851651490956826 1105 90-91 -0.3061321128799044 1105 92-93 -0.014275888508393564 1105 94-95 0.19381519366850952 1105 96-97 -0.031518452992997936 1105 98-99 0.3932261592800792 1105 100-101 0.8992735389623583 1106 1 0.16579033358436135 1106 2 0.15409308210242756 1106 3 0.0978930333771828 1106 4 0.20075800029449908 1106 5 0.01334441485224147 1106 6 0.10182494814264231 1106 7 -0.06444760791341508 1106 8 0.0051393065924969505 1106 9 0.13078099135154275 1106 10-11 0.23297820790136825 1106 12-13 0.22259916656840772 1106 14-15 0.42155978593292787 1106 16-17 0.5726928784184793 1106 18-19 0.2795514712750915 1106 20-21 0.32938134985779044 1106 22-23 0.3307761805010614 1106 24-25 0.3893067016176204 1106 26-27 0.18617306086314755 1106 28-29 -0.12530093320948055 1106 30-31 0.10797725150319337 1106 32-33 0.4340747467068553 1106 34-35 0.10227033226418314 1106 36-37 0.16631672087076055 1106 38-39 0.5808702000025505 1106 40-41 0.7001091877764978 1106 42-43 0.7186950697298684 1106 44-45 0.7170515079207718 1106 46-47 0.06289787805358316 1106 48-49 -0.2600585532273598 1106 50-51 -0.3971474613749635 1106 52-53 -0.8203244204049653 1106 54-55 -0.7944185039615164 1106 56-57 -0.5682627018810926 1106 58-59 -0.2893602150877612 1106 60-61 -0.3614521148187393 1106 62-63 -0.2898406124013526 1106 64-65 -0.04394517810671417 1106 66-67 -0.34088995604379235 1106 68-69 -0.8135057669908576 1106 70-71 -0.37129388709583466 1106 72-73 -0.34515219929521024 1106 74-75 -0.09334985636359505 1106 76-77 0.2692829959430689 1106 78-79 0.23564504908893014 1106 80-81 0.08386344923278699 1106 82-83 0.28592811664888274 1106 84-85 0.2725308603402503 1106 86-87 0.563277732717733 1106 88-89 0.5595569669103462 1106 90-91 0.48427336970148005 1106 92-93 0.9159544560566175 1106 94-95 1.1564317937065844 1106 96-97 0.9835443682520051 1106 98-99 1.1318755025125178 1106 100-101 1.9344677137196378 1107 1 0.0278987783544693 1107 2 0.03484047173422766 1107 3 0.30337938618905724 1107 4 0.0519670129341776 1107 5 0.15334258301491133 1107 6 0.10828217474092128 1107 7 0.20748864414740353 1107 8 0.3259398195069494 1107 9 0.18514076420371595 1107 10-11 0.15972761255424217 1107 12-13 -0.026301731031885822 1107 14-15 0.3309525318570863 1107 16-17 0.29434519569271345 1107 18-19 0.16055073854015944 1107 20-21 0.24939875231245168 1107 22-23 0.21385916273224836 1107 24-25 0.17573736657457317 1107 26-27 0.09018478462208179 1107 28-29 0.049593736143883405 1107 30-31 -0.024624873428109595 1107 32-33 -0.3441813441522754 1107 34-35 -0.2556248866403763 1107 36-37 -0.2149573219942269 1107 38-39 0.09101399923316933 1107 40-41 -0.17656236378972068 1107 42-43 -0.24791969487903032 1107 44-45 -0.13282942265259123 1107 46-47 -0.15978484772037405 1107 48-49 -0.8188147356783588 1107 50-51 -0.7963048162018609 1107 52-53 -0.7935337994121099 1107 54-55 -1.0180400463685508 1107 56-57 -0.5918018116081498 1107 58-59 -0.372617221865557 1107 60-61 -0.11566534068428069 1107 62-63 0.06835228006979222 1107 64-65 -0.28494627720911225 1107 66-67 0.08549308114250564 1107 68-69 0.4036356508512391 1107 70-71 0.22794590041103646 1107 72-73 -0.06859055796895674 1107 74-75 0.8287512610571781 1107 76-77 0.6726306786688419 1107 78-79 0.5982343511589043 1107 80-81 0.81059911510566 1107 82-83 0.6415793348207082 1107 84-85 1.1512861268725807 1107 86-87 1.096205008775616 1107 88-89 1.3251275842395245 1107 90-91 1.558462781681694 1107 92-93 2.0164307337631584 1107 94-95 1.989551412684417 1107 96-97 1.9114615692045582 1107 98-99 1.8278820971269134 1107 100-101 2.2082358031133005 1108 1 0.22240992758259637 1108 2 0.09504630363817768 1108 3 0.17507749939660044 1108 4 0.2662037196237108 1108 5 0.13267362932708693 1108 6 0.13452574249392057 1108 7 0.14093632853848703 1108 8 0.13099985381226276 1108 9 0.14088690485551325 1108 10-11 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-1.010209992190699 2316 78-79 -0.5439773888830253 2316 80-81 -0.9470319121480131 2316 82-83 -0.704395507946586 2316 84-85 -0.7236719444062452 2316 86-87 -0.398847402125849 2316 88-89 -0.4736145541252519 2316 90-91 -0.6565896292197699 2316 92-93 -1.083883731645649 2316 94-95 -1.497786113521034 2316 96-97 -1.9611589758688162 2316 98-99 -2.617002598889858 2316 100-101 -3.096837572148754 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 7.0 5 10.0 6 41.0 7 82.0 8 152.0 9 259.0 10 455.0 11 695.0 12 976.0 13 1538.0 14 1930.0 15 2502.0 16 3169.0 17 4312.0 18 4208.0 19 3229.0 20 2786.0 21 2891.0 22 3011.0 23 3057.0 24 3426.0 25 3276.0 26 3037.0 27 2932.0 28 3464.0 29 4059.0 30 4825.0 31 5638.0 32 6827.0 33 8587.0 34 12749.0 35 23799.0 36 23000.0 37 33951.0 38 23667.0 39 1490.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.815760926619305 23.271462064897346 16.188343273061754 15.724433735421592 2 15.228010179338344 26.384784988975717 38.27451215670981 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35.708286884057614 16.666749983753167 22-23 16.487035028169508 31.526602312549052 36.38915411494759 15.597208544333855 24-25 17.22952790643614 32.9910590423152 35.77082953190575 14.008583519342913 26-27 15.95123926858903 32.34348515227728 37.98231973479602 13.722955844337664 28-29 14.019336326098042 34.15151121286956 36.964476749417614 14.864675711614794 30-31 15.260524197781432 33.02780957713246 36.618609371172624 15.093056853913486 32-33 15.289303711406173 33.41640337545992 37.52299632058871 13.771296592545193 34-35 14.977225797097102 32.93184738534151 37.47793827216048 14.612988545400912 36-37 14.88763904511936 32.56167979002625 37.76827896512936 14.782402199725034 38-39 14.178856341422561 33.11749838123786 37.67128253461901 15.03236274272057 40-41 13.788008340458724 33.404087224797365 38.0530929201106 14.754811514633303 42-43 14.056359436405636 33.29491705082949 36.448885511144894 16.199838001619984 44-45 14.065308305898647 32.48292050064868 37.0816991258396 16.37007206761307 46-47 16.135885880256254 32.96989784313284 35.768151153170315 15.126065123440588 48-49 15.22503111893181 32.161728462949725 36.27767585320862 16.335564564909845 50-51 12.91248206599713 32.94282614890097 37.763136188443255 16.38155559665865 52-53 14.128244992226517 33.89389069131519 35.75727733092047 16.220586985537818 54-55 13.933033058553582 32.495413394388095 35.820265048315584 17.751288498742746 56-57 15.863406635706037 32.51041046995836 34.03311354285915 17.593069351476462 58-59 15.926394353101145 32.780107878963605 34.739975704737574 16.553522063197676 60-61 15.17953998970201 34.15733931883283 34.589255095256426 16.07386559620874 62-63 13.775313813806308 34.84495523372942 34.31280900224456 17.066921950219708 64-65 13.731322392133535 35.51057543778963 33.47422252660731 17.283879643469525 66-67 14.535488257231128 33.79482898249332 32.82626301002809 18.843419750247453 68-69 14.03208390405823 33.60286545556344 33.50013497435487 18.864915666023453 70-71 15.892650933068051 33.17303125890451 32.49741300446413 18.436904803563305 72-73 15.527972045451138 31.203665285269373 34.594754022965525 18.67360864631397 74-75 14.554911792200521 33.181779552987166 32.75336309419663 19.50994556061568 76-77 14.621648778488193 31.56959392918381 33.888891666125105 19.91986562620289 78-79 14.873849599328132 31.219412214618146 34.14609151215513 19.76064667389859 80-81 15.675810785477722 30.807223645566456 33.300756108229706 20.216209460726116 82-83 14.908141075311821 30.438923188442025 34.49621316269653 20.15672257354963 84-85 14.968616972081477 30.539148171385577 33.727943768013056 20.764291088519887 86-87 15.082035233657942 31.21688096142694 33.29150752864484 20.409576276270272 88-89 15.014572158429107 30.633026559820838 32.162728267987745 22.189673013762317 90-91 15.623468949036626 29.1639586853709 33.21493205883235 21.997640306760122 92-93 16.434209671210827 29.916715407649587 33.12387208366452 20.525202837475067 94-95 15.885402346542424 28.886617109663614 33.527962047400756 21.700018496393202 96-97 14.999075180339833 29.22805053014662 33.914386694594555 21.85848759491899 98-99 15.640989109596404 29.45982898292079 32.1938026929816 22.70537921450121 100-101 15.21498952672809 28.606779881319582 33.32025215839386 22.85797843355846 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4465.0 1 2661.0 2 1093.0 3 1301.0 4 951.5 5 857.5 6 1715.0 7 3216.5 8 4330.0 9 4437.0 10 3300.0 11 2045.5 12 1735.5 13 1628.0 14 1474.5 15 1267.0 16 1132.5 17 1101.0 18 1113.0 19 1131.0 20 1161.5 21 1206.5 22 1267.5 23 1353.0 24 1422.5 25 1583.5 26 1930.5 27 2367.5 28 2583.5 29 2800.5 30 3353.5 31 3917.0 32 4629.5 33 5249.0 34 5566.5 35 6027.0 36 6444.0 37 6525.0 38 6138.0 39 5710.0 40 5536.5 41 5443.0 42 5066.0 43 5288.0 44 5780.5 45 5242.5 46 4749.0 47 6692.0 48 10930.0 49 10342.5 50 6115.0 51 4457.5 52 3969.5 53 3230.5 54 2097.5 55 1185.0 56 774.5 57 607.5 58 515.0 59 425.5 60 349.5 61 292.5 62 216.0 63 142.5 64 114.5 65 95.5 66 73.5 67 59.0 68 47.5 69 34.5 70 24.0 71 26.5 72 34.5 73 37.0 74 31.5 75 25.5 76 17.0 77 7.0 78 2.5 79 0.5 80 0.5 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.012997465494228626 3 0.004999025190087933 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00224956133553957 26-27 0.02099590579836932 28-29 4.999025190087933E-4 30-31 0.0 32-33 0.003499317633061553 34-35 0.015996880608281385 36-37 0.006998635266123106 38-39 0.020246052019856128 40-41 0.024995125950439664 42-43 0.01849639320332535 44-45 0.009248196601662675 46-47 0.0017496588165307764 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.999025190087933E-4 68-69 4.999025190087933E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 7.4985377851319E-4 82-83 0.001999610076035173 84-85 0.00524897644959233 86-87 0.00149970755702638 88-89 0.0 90-91 0.006498732747114313 92-93 9.998050380175866E-4 94-95 0.0 96-97 0.0 98-99 2.4995125950439664E-4 100-101 0.00299941511405276 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 200039.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.84181584591005 #Duplication Level Percentage of deduplicated Percentage of total 1 78.61950120763115 28.96485185388849 2 9.998914488859942 7.367563325151595 3 3.681239653721946 4.068706602212568 4 1.8752204944503243 2.7634611250806094 5 1.1791364758880838 2.172076445093207 6 0.7490026866400716 1.6556771429571233 7 0.5413986811039648 1.3962277355915595 8 0.43013378924801215 1.2677527882062998 9 0.32293956416727726 1.0707911957168352 >10 2.2755027273467396 15.728932858092673 >50 0.20896089446117938 5.353955978584176 >100 0.08141333550435562 5.918345922545103 >500 0.01899644495101631 4.991526652302801 >1k 0.014925778175798531 11.637730642524707 >5k 0.002713777850145187 5.64239973205225 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5906 2.952424277265933 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5381 2.6899754547863166 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4874 2.4365248776488584 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3775 1.8871320092581947 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2724 1.361734461779953 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 2005 1.0023045506126305 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1976 0.9878073775613755 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1788 0.8938257039877224 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1459 0.7293577752338294 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1301 0.6503731772304401 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 1181 0.5903848749493849 TruSeq Adapter, Index 10 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1113 0.5563915036567869 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1084 0.5418943306055319 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 930 0.46490934267817774 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 917 0.4584106099310635 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 871 0.4354150940566589 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 836 0.4179185058913512 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 771 0.3854248421557796 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 742 0.3709276691045246 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 724 0.3619294237623663 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 689 0.34443283559705856 No Hit ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 682 0.340933517963997 RNA PCR Primer, Index 10 (95% over 23bp) ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 638 0.3189378071276101 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 614 0.3069401466713991 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 539 0.2694474577457396 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 530 0.2649483350746604 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 502 0.2509510645424142 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 472 0.23595398897215042 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 450 0.224956133553957 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 441 0.22045701088287784 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 438 0.21895730332585148 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 433 0.2164577907308075 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 370 0.1849639320332535 No Hit CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 343 0.1714665640200161 TruSeq Adapter, Index 10 (96% over 26bp) GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 305 0.15247026829768195 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 303 0.15147046325966437 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 298 0.1489709506646204 TruSeq Adapter, Index 10 (96% over 28bp) AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 285 0.14247221791750608 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 275 0.13747319272741815 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 265 0.1324741675373302 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 254 0.1269752398282335 No Hit TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC 253 0.1264753373092247 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 249 0.12447572723318953 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 235 0.11747709196706642 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 232 0.11597738441004005 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 229 0.11447767685301366 No Hit ACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 214 0.10697913906788177 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 212 0.10597933402986418 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 204 0.10198011387779382 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 201 0.10048040632076745 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.4995125950439664E-4 0.0 18-19 0.0 0.0 0.0 0.0012497562975219833 0.0 20-21 0.0 0.0 0.0 0.0024995125950439665 0.0 22-23 0.0 0.0 0.0 0.009748099120671469 0.0 24-25 0.0 0.0 0.0 0.023495418393413285 0.0 26-27 0.0 0.0 0.0 0.05723883842650683 0.0 28-29 0.0 0.0 0.0 0.12497562975219834 0.0 30-31 0.0 0.0 0.0 0.2731967266383055 0.0 32-33 0.0 0.0 0.0 0.5089007643509516 0.0 34-35 0.0 0.0 0.0 0.8353371092636935 0.0 36-37 0.0 0.0 0.0 1.2547553227120711 0.0 38-39 0.0 0.0 0.0 1.7296627157704247 0.0 40-41 0.0 0.0 0.0 2.1775753728023037 0.0 42-43 0.0 0.0 0.0 2.576747534230825 0.0 44-45 0.0 0.0 0.0 3.014412189623023 0.0 46-47 0.0 0.0 0.0 3.5065662195871807 0.0 48-49 0.0 0.0 0.0 3.9444808262388835 0.0 50-51 0.0 0.0 0.0 4.37064772369388 0.0 52-53 0.0 0.0 0.0 4.727078219747149 0.0 54-55 0.0 0.0 0.0 5.136998285334359 0.0 56-57 0.0 0.0 0.0 5.641149975754727 0.0 58-59 0.0 0.0 0.0 6.098560780647773 0.0 60-61 0.0 0.0 0.0 6.552472267907758 0.0 62-63 0.0 0.0 0.0 6.981138677957798 0.0 64-65 0.0 0.0 0.0 7.4043061602987414 0.0 66-67 0.0 0.0 0.0 7.871964966831468 0.0 68-69 0.0 0.0 0.0 8.413359394917991 0.0 70-71 0.0 0.0 0.0 8.935257624763171 0.0 72-73 0.0 0.0 0.0 9.390668819580181 0.0 74-75 0.0 0.0 0.0 9.932813101445218 0.0 76-77 0.0 0.0 0.0 10.43096596163748 0.0 78-79 0.0 0.0 0.0 11.032348692005058 0.0 80-81 0.0 0.0 0.0 11.644229375271822 0.0 82-83 0.0 0.0 0.0 12.182874339503798 0.0 84-85 0.0 0.0 0.0 12.747264283464723 0.0 86-87 0.0 0.0 0.0 13.291158224146292 0.0 88-89 0.0 0.0 0.0 13.895290418368418 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATACAG 40 5.377587E-4 47.49975 5 CTCTAGG 65 1.322358E-4 36.54741 2 TCTCTAG 55 0.0025714652 34.55391 1 ATAGGGT 110 6.963637E-8 34.545273 3 TAATACC 70 2.0473578E-4 33.928394 4 CATAGGG 130 8.194547E-9 32.89267 2 TCCTGAT 90 2.3990631E-5 31.674417 2 CAGGTAC 30 0.003948002 31.666498 40-41 AGGGTCT 120 1.4953548E-7 31.666498 5 GGGAGGG 60 0.0039433236 31.666498 7 CTAGTCA 60 0.0039433236 31.666498 9 TAGGGTC 120 1.4953548E-7 31.666498 4 ATAACAT 60 0.0039433236 31.666498 3 GTCCTAG 125 2.134293E-7 30.40744 1 ATGAGAG 80 4.4806645E-4 29.687344 5 ATCTAGA 65 0.0058257114 29.237926 1 TCCAGAG 65 0.0058257114 29.237926 2 ATCCAGA 65 0.0058257114 29.237926 1 CTGATCC 100 4.9331313E-5 28.499851 4 CCTGATC 100 4.9331313E-5 28.499851 3 >>END_MODULE