##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938040_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 210189 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.473507176874147 33.0 31.0 34.0 30.0 34.0 2 31.51091160812412 33.0 31.0 34.0 30.0 34.0 3 31.459781434803915 34.0 31.0 34.0 28.0 34.0 4 34.94856533881411 37.0 35.0 37.0 33.0 37.0 5 34.97546018107513 37.0 35.0 37.0 33.0 37.0 6 35.11481095585402 37.0 35.0 37.0 33.0 37.0 7 35.07522277569235 37.0 35.0 37.0 33.0 37.0 8 35.0886154841595 37.0 35.0 37.0 33.0 37.0 9 36.634367164789786 39.0 37.0 39.0 33.0 39.0 10-11 36.59108231163381 39.0 37.0 39.0 33.0 39.0 12-13 36.4678218175071 39.0 37.0 39.0 33.0 39.0 14-15 37.471461399026595 40.0 38.0 41.0 32.5 41.0 16-17 37.356783656613814 40.0 38.0 41.0 32.5 41.0 18-19 37.5321686672471 40.0 38.0 41.0 33.0 41.0 20-21 37.582192693242746 40.0 38.0 41.0 33.0 41.0 22-23 37.41784536774046 40.0 37.5 41.0 33.0 41.0 24-25 37.233492237938236 40.0 37.0 41.0 33.0 41.0 26-27 36.958932199116035 40.0 36.5 41.0 32.5 41.0 28-29 36.71382660367574 40.0 35.0 41.0 32.0 41.0 30-31 36.32225996603057 39.0 35.0 41.0 31.5 41.0 32-33 35.77376313698623 38.0 35.0 41.0 30.5 41.0 34-35 35.40109853512791 38.0 35.0 40.5 30.0 41.0 36-37 35.06971820599556 38.0 35.0 40.0 29.0 41.0 38-39 34.72270908563246 38.0 35.0 40.0 26.0 41.0 40-41 34.40172654135088 38.0 35.0 40.0 23.5 41.0 42-43 33.9821612929316 38.0 35.0 40.0 19.5 41.0 44-45 33.4556898791088 37.5 34.5 40.0 12.0 41.0 46-47 32.803719509584226 37.0 33.0 40.0 2.0 41.0 48-49 32.15991322095828 37.0 33.0 40.0 2.0 41.0 50-51 31.33317871058904 36.0 32.0 39.5 2.0 40.5 52-53 31.369838573854956 36.0 32.5 39.5 2.0 40.5 54-55 31.423183420635716 36.0 33.0 40.0 2.0 41.0 56-57 31.102612410735098 36.0 32.0 40.0 2.0 41.0 58-59 30.754696963209305 35.5 31.0 40.0 2.0 41.0 60-61 30.272212151920414 35.0 30.5 39.5 2.0 41.0 62-63 29.809559491695566 35.0 29.0 39.0 2.0 41.0 64-65 29.36424598813449 35.0 28.0 39.0 2.0 40.5 66-67 28.912022037309278 35.0 27.5 38.5 2.0 40.0 68-69 28.334144508038 35.0 26.0 37.5 2.0 40.0 70-71 27.81804471214003 35.0 25.5 37.0 2.0 39.0 72-73 27.20778680140255 34.5 23.0 36.0 2.0 39.0 74-75 26.664180808700742 34.0 20.5 36.0 2.0 38.5 76-77 26.06126628891141 34.0 15.5 35.0 2.0 37.5 78-79 25.406819576666713 33.0 7.0 35.0 2.0 37.0 80-81 24.720539609589466 33.0 2.0 35.0 2.0 36.0 82-83 24.075184714709145 32.5 2.0 35.0 2.0 36.0 84-85 23.43880745424356 32.0 2.0 35.0 2.0 35.5 86-87 22.866025339099572 31.0 2.0 35.0 2.0 35.0 88-89 22.08739515388531 31.0 2.0 34.5 2.0 35.0 90-91 21.32618262611269 29.5 2.0 34.0 2.0 35.0 92-93 20.564268348962123 29.0 2.0 34.0 2.0 35.0 94-95 19.687997468944616 28.0 2.0 34.0 2.0 35.0 96-97 18.655003354124148 25.5 2.0 34.0 2.0 35.0 98-99 17.46873052348125 21.0 2.0 33.5 2.0 35.0 100-101 13.987102084314593 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2105408375694715 1101 2 -0.05067045626784861 1101 3 0.02335142608919938 1101 4 0.3032747632320252 1101 5 0.1784685678523843 1101 6 0.014778607672099042 1101 7 0.12295423815167794 1101 8 0.1625617393417329 1101 9 0.35206521659122103 1101 10-11 0.08482366077883796 1101 12-13 0.12179550167493147 1101 14-15 0.1895261636644463 1101 16-17 0.2622947813880998 1101 18-19 -0.13202336016474447 1101 20-21 -0.055570975123274025 1101 22-23 -0.24355869040148548 1101 24-25 0.22159883569215566 1101 26-27 0.25855507257090693 1101 28-29 0.19292659892883535 1101 30-31 0.251038696339819 1101 32-33 0.5656720893102047 1101 34-35 0.695301635045972 1101 36-37 0.497974753390821 1101 38-39 0.31277074033021535 1101 40-41 0.6933696389859065 1101 42-43 0.020235677445583633 1101 44-45 0.616490214345788 1101 46-47 1.1032665354276006 1101 48-49 1.1253063032982027 1101 50-51 0.9267527275572256 1101 52-53 0.8975607649778219 1101 54-55 1.4418046402809104 1101 56-57 1.7549080069183738 1101 58-59 1.5939787503347418 1101 60-61 1.1281480559808479 1101 62-63 0.6822121936227745 1101 64-65 0.6438763410424677 1101 66-67 0.5943837346928582 1101 68-69 0.20194605276362765 1101 70-71 0.3613551545158842 1101 72-73 0.20045658295387625 1101 74-75 0.10615260465734622 1101 76-77 -0.12027397366286863 1101 78-79 -0.23829809434825577 1101 80-81 0.27633906696907573 1101 82-83 0.9227268258148413 1101 84-85 0.9336441945678722 1101 86-87 0.8825672398056987 1101 88-89 0.8889975149184259 1101 90-91 0.7762216140733607 1101 92-93 0.4849763008571273 1101 94-95 0.2014187037571915 1101 96-97 0.5459985060619061 1101 98-99 0.6718871364454273 1101 100-101 -0.48321205097156117 1102 1 0.27142809845673455 1102 2 0.07313906754167832 1102 3 0.295390498128274 1102 4 0.2996117595690251 1102 5 0.16861915800297567 1102 6 0.4664676593611503 1102 7 0.27606779126523406 1102 8 0.15377053055052414 1102 9 0.21295247747848123 1102 10-11 0.2223897983449703 1102 12-13 0.42606900594842756 1102 14-15 0.7019396760779628 1102 16-17 0.23018244927576603 1102 18-19 0.12259609445470687 1102 20-21 0.04304522349292483 1102 22-23 0.4669419200991243 1102 24-25 0.5375532516465711 1102 26-27 0.32143663545247847 1102 28-29 0.42927173527397144 1102 30-31 0.3956459409470625 1102 32-33 0.5313619550000723 1102 34-35 0.4325014722458107 1102 36-37 0.45658281199887796 1102 38-39 0.21545694301642015 1102 40-41 0.10614945176571666 1102 42-43 0.149336206546117 1102 44-45 -0.1457401478845739 1102 46-47 0.37123560339666284 1102 48-49 0.550377528369431 1102 50-51 1.1379045387090407 1102 52-53 0.7048053722224275 1102 54-55 0.7148612133374748 1102 56-57 0.9024049544153208 1102 58-59 0.7901936465496391 1102 60-61 0.8136995415323334 1102 62-63 0.61802822943881 1102 64-65 0.4897859869521177 1102 66-67 0.3238912642003875 1102 68-69 0.8403261911437703 1102 70-71 0.6686404618011927 1102 72-73 0.9368831193804112 1102 74-75 1.0161241146288589 1102 76-77 0.5986515452626477 1102 78-79 0.2457474896973295 1102 80-81 0.40975380038381104 1102 82-83 -0.0101995071114942 1102 84-85 0.4281496890733649 1102 86-87 0.6018997591382202 1102 88-89 0.6478090737299844 1102 90-91 0.42351506136680683 1102 92-93 0.41908293496376103 1102 94-95 0.36405606639455357 1102 96-97 0.05263261269600861 1102 98-99 0.20192376648206078 1102 100-101 -0.29289866065816916 1103 1 0.2420979048408256 1103 2 0.49864927162331085 1103 3 0.6282267095359124 1103 4 0.5379686407830491 1103 5 0.39237635318874453 1103 6 0.46848231137580143 1103 7 0.5192127415530408 1103 8 0.42276582097438364 1103 9 0.616615481141487 1103 10-11 0.728342179297357 1103 12-13 0.3499701048495325 1103 14-15 0.3361625960151642 1103 16-17 0.1863310625672412 1103 18-19 0.3692993911580018 1103 20-21 0.6188432350052295 1103 22-23 0.7431193141336578 1103 24-25 0.6993010276800646 1103 26-27 0.3328181111196642 1103 28-29 0.5688714213022266 1103 30-31 0.7722027175038377 1103 32-33 0.853653948720634 1103 34-35 0.9765752556053116 1103 36-37 1.211410127540482 1103 38-39 0.9017730079039197 1103 40-41 0.8893257992277768 1103 42-43 0.962601512668563 1103 44-45 1.0489354146481347 1103 46-47 1.0894721287760447 1103 48-49 1.2913115943034938 1103 50-51 2.028132168222381 1103 52-53 2.0513778473663358 1103 54-55 2.140122856456262 1103 56-57 1.9915860114535242 1103 58-59 2.3102485916045765 1103 60-61 2.514863853410926 1103 62-63 2.465280976691556 1103 64-65 1.9344092208610668 1103 66-67 1.9785485117147807 1103 68-69 2.015627080730372 1103 70-71 1.4238079133972157 1103 72-73 0.9540469079727742 1103 74-75 0.673423957642985 1103 76-77 0.9318802213484716 1103 78-79 1.188761618425744 1103 80-81 1.0187804879819247 1103 82-83 -0.06149464054948339 1103 84-85 -0.5918660095137618 1103 86-87 -1.0537517322275605 1103 88-89 -0.7463955311889059 1103 90-91 -0.32789781147463515 1103 92-93 -0.07322475734392953 1103 94-95 0.17250713913134064 1103 96-97 -0.0249576541799712 1103 98-99 -0.36807884994912854 1103 100-101 0.1807146308122629 1104 1 0.32469034775858674 1104 2 0.31472322595751834 1104 3 0.4817829835306604 1104 4 0.6066693884088323 1104 5 0.6537422857003605 1104 6 0.7474780680943311 1104 7 0.7146562654972684 1104 8 0.614471216003686 1104 9 0.49426753206086715 1104 10-11 0.6341059699621354 1104 12-13 0.5700903311578784 1104 14-15 0.6507210926811595 1104 16-17 0.5189740207406075 1104 18-19 0.8130759116672053 1104 20-21 1.0034158759427854 1104 22-23 1.2066220419574663 1104 24-25 1.1584341858938458 1104 26-27 0.8319088365187355 1104 28-29 0.8873448502777777 1104 30-31 0.9606751361742809 1104 32-33 0.9109887638348013 1104 34-35 0.5214580602123036 1104 36-37 0.531449529439854 1104 38-39 0.7136036807671218 1104 40-41 -0.18089040606621865 1104 42-43 -0.1313568627608177 1104 44-45 -0.6650724657317468 1104 46-47 -0.4509400757097026 1104 48-49 -0.5409019841972089 1104 50-51 -0.615103839051816 1104 52-53 -0.790256826305118 1104 54-55 -0.8645498046874032 1104 56-57 -0.9176128165925483 1104 58-59 -1.172601479116775 1104 60-61 -1.3521547362031292 1104 62-63 -0.9887501407058963 1104 64-65 -1.1883175926366114 1104 66-67 -0.8979416883256341 1104 68-69 -0.6521845983967225 1104 70-71 -0.5487370451802782 1104 72-73 -0.36485590067543683 1104 74-75 -1.4006314070771602 1104 76-77 -1.5464487339960442 1104 78-79 -0.8269486260680914 1104 80-81 -1.2160649353359148 1104 82-83 -1.564045660957646 1104 84-85 -0.9726500062807872 1104 86-87 -0.7374757168709216 1104 88-89 -0.16827368839238233 1104 90-91 0.11688901414670028 1104 92-93 -0.20688812368056375 1104 94-95 0.211555431715702 1104 96-97 -0.24416860588738842 1104 98-99 -0.5309652916545247 1104 100-101 -0.833505413641161 1105 1 0.5423179928760433 1105 2 0.6054920087181479 1105 3 0.5312879340257091 1105 4 0.5273643990863661 1105 5 0.6230686300995103 1105 6 0.5819276895270633 1105 7 0.9716937339499978 1105 8 0.9013572575490159 1105 9 0.7426659013095573 1105 10-11 0.879252543443009 1105 12-13 1.0106843905638172 1105 14-15 1.2596319837702694 1105 16-17 1.1058988896980892 1105 18-19 1.1651677384969403 1105 20-21 1.0832790093149498 1105 22-23 1.011926837142866 1105 24-25 1.075901667936165 1105 26-27 1.1674019446530792 1105 28-29 1.0015945105379203 1105 30-31 1.041460420585075 1105 32-33 0.7520633124661344 1105 34-35 0.5317473244328426 1105 36-37 0.6674425405056681 1105 38-39 0.7162487982200432 1105 40-41 0.8464586523690301 1105 42-43 0.6375715006637677 1105 44-45 0.5621406967609701 1105 46-47 0.491182813049754 1105 48-49 0.9744348436032197 1105 50-51 0.8945787468538349 1105 52-53 1.0545791278785366 1105 54-55 0.5514373822665881 1105 56-57 0.8304215456672033 1105 58-59 1.083707975358081 1105 60-61 0.7149438854237395 1105 62-63 0.505547083134136 1105 64-65 0.7879006174196874 1105 66-67 0.6857313848640381 1105 68-69 0.816947608941657 1105 70-71 0.8922452883471941 1105 72-73 0.9137307815221938 1105 74-75 1.4081678552020094 1105 76-77 0.9090210776910084 1105 78-79 0.9711245636038157 1105 80-81 0.8770237853008567 1105 82-83 1.3575229664933346 1105 84-85 1.1031119816827193 1105 86-87 0.9030309808576753 1105 88-89 0.37145160766663565 1105 90-91 0.14169002365941452 1105 92-93 0.3238340661854835 1105 94-95 0.6096820090793216 1105 96-97 0.6243520697095875 1105 98-99 0.9887638871457085 1105 100-101 0.39069862440970304 1106 1 -0.33284540581676936 1106 2 0.05091684531945617 1106 3 0.1590657118034855 1106 4 0.02867158862885333 1106 5 -0.008303918920098852 1106 6 -0.06088276798927694 1106 7 -0.03418861899118042 1106 8 -0.08981921303922036 1106 9 0.15312341764942516 1106 10-11 0.2029453539005317 1106 12-13 0.17457327945270862 1106 14-15 0.095743094881378 1106 16-17 0.07419954329286327 1106 18-19 -0.19300219614358127 1106 20-21 -0.26998896454126253 1106 22-23 -0.21062218246498077 1106 24-25 -0.45154931245598817 1106 26-27 -0.48668302266718655 1106 28-29 -0.4147453587431258 1106 30-31 -0.657559187258066 1106 32-33 -0.2966294979913826 1106 34-35 -0.41514810040376204 1106 36-37 -0.2694855640694911 1106 38-39 -0.31937211681263733 1106 40-41 -0.8440642234479583 1106 42-43 -0.5228860156761073 1106 44-45 -0.5921076692520941 1106 46-47 -0.6405165333554734 1106 48-49 -0.07932332633141925 1106 50-51 -0.044146743342246 1106 52-53 -0.061433944016886954 1106 54-55 0.20503215350841586 1106 56-57 0.1763365783469446 1106 58-59 0.3876295439855362 1106 60-61 0.6252380030707947 1106 62-63 0.4434555798661606 1106 64-65 0.36927316643929586 1106 66-67 0.8450451103542349 1106 68-69 0.5937449945625737 1106 70-71 0.3564609746217009 1106 72-73 0.6926523501496433 1106 74-75 0.14967112317586384 1106 76-77 -0.24963905302794842 1106 78-79 0.25429449824433803 1106 80-81 0.3706512362812475 1106 82-83 0.5803987834867996 1106 84-85 0.6161838771075523 1106 86-87 0.14698522922368795 1106 88-89 -0.5301823792614684 1106 90-91 -1.053408015556272 1106 92-93 -1.164891424010598 1106 94-95 -1.2600892327507438 1106 96-97 -1.1022819172185194 1106 98-99 -0.8491446095863182 1106 100-101 -1.3424713102308186 1107 1 0.377335700399577 1107 2 0.2352536049866103 1107 3 0.21410731728562027 1107 4 0.563815984125668 1107 5 0.5536637756467115 1107 6 0.32829980665585623 1107 7 0.33335910494420773 1107 8 0.3261866606759085 1107 9 0.1811214597443822 1107 10-11 0.45293311699402494 1107 12-13 0.37191787074002747 1107 14-15 0.4589711908187297 1107 16-17 0.567028716077985 1107 18-19 0.7605832174638536 1107 20-21 0.7320545988459131 1107 22-23 0.8400018997670387 1107 24-25 0.8194125083956934 1107 26-27 0.6094745886886628 1107 28-29 0.530898302539299 1107 30-31 0.3307300932118338 1107 32-33 0.3613856757726879 1107 34-35 0.5180995745840065 1107 36-37 0.4930450281967822 1107 38-39 0.5373704186743922 1107 40-41 0.07126862040911419 1107 42-43 -0.2105756877270153 1107 44-45 -0.035585398082247366 1107 46-47 0.6059470006631287 1107 48-49 0.11266149990450458 1107 50-51 0.25688115680459944 1107 52-53 0.05151270307072764 1107 54-55 -0.039583607627168504 1107 56-57 0.05590094447518723 1107 58-59 -0.24159218876042843 1107 60-61 -0.004791365504829059 1107 62-63 -0.1001689918421107 1107 64-65 -0.3244125898015291 1107 66-67 -0.10557897187777243 1107 68-69 -0.10025892124751223 1107 70-71 0.07132881603139296 1107 72-73 0.3582544059499355 1107 74-75 -0.571608857245085 1107 76-77 -0.7845631842075846 1107 78-79 -0.7781088301942312 1107 80-81 -0.32453965946031005 1107 82-83 0.21128473551812377 1107 84-85 -0.7217994806995911 1107 86-87 -0.6436780111091558 1107 88-89 -0.3431657370686132 1107 90-91 -0.12433312568843036 1107 92-93 0.06333911930805414 1107 94-95 -0.05178037323042872 1107 96-97 -0.6286279769346237 1107 98-99 -0.45691504522019244 1107 100-101 -1.0043411095455532 1108 1 0.2382657052943422 1108 2 0.4820279564305672 1108 3 0.3401768229145965 1108 4 0.4442271441844099 1108 5 0.6339183033021243 1108 6 0.7902283431218393 1108 7 0.5991447143421524 1108 8 0.3390696758496716 1108 9 0.6020123065383132 1108 10-11 0.5223897983449746 1108 12-13 0.43512883500826405 1108 14-15 0.5296319837702654 1108 16-17 0.524199543292859 1108 18-19 0.6942200260786393 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0.8565996961547988 2316 56-57 0.7740083164751148 2316 58-59 1.5321661968209455 2316 60-61 1.6008258315631885 2316 62-63 1.6505210478237444 2316 64-65 1.4575165965914358 2316 66-67 1.4728113620831422 2316 68-69 1.2262715044749761 2316 70-71 0.9739920949536547 2316 72-73 1.1682056004954262 2316 74-75 1.0792584906263016 2316 76-77 0.9259948796599247 2316 78-79 1.0412470109229908 2316 80-81 0.8661214990751596 2316 82-83 1.0811825548099883 2316 84-85 1.4884752556229977 2316 86-87 1.6336265385828384 2316 88-89 0.9708434390880036 2316 90-91 1.129994474070287 2316 92-93 1.00976279452329 2316 94-95 0.17731279583385628 2316 96-97 -0.034727744788824566 2316 98-99 -0.4542737013336655 2316 100-101 0.08094962107392867 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4450.0 3 657.0 4 507.0 5 476.0 6 407.0 7 426.0 8 492.0 9 569.0 10 651.0 11 925.0 12 1289.0 13 1724.0 14 2088.0 15 2866.0 16 3516.0 17 4503.0 18 4157.0 19 2927.0 20 2860.0 21 2754.0 22 2889.0 23 2870.0 24 3058.0 25 3347.0 26 3853.0 27 4125.0 28 4686.0 29 5503.0 30 6512.0 31 7651.0 32 9143.0 33 11131.0 34 15936.0 35 25444.0 36 21800.0 37 26863.0 38 15980.0 39 1154.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.33528060762582 22.96036518540402 15.865361706993985 14.838992499976175 2 15.797665740070826 26.750694946879406 37.79321046418644 19.658428848863334 3 20.7754398002554 31.32492805001239 32.68292450492691 15.216707644805306 4 11.336989094651186 22.74353611533276 38.30021391444375 27.619260875572305 5 8.807040292900593 43.18615205659694 33.92383821818806 14.08296943231441 6 22.455245405353867 41.72677647731878 17.375032776334294 18.442945340993063 7 20.20434519469503 42.29821626850806 21.927078121053558 15.570360415743348 8 24.070433329683773 32.77939363358103 20.596369798782316 22.55380323795288 9 25.223513048779907 20.720386009535922 23.78312208551845 30.27297885616572 10-11 23.07855333092031 30.70957079991618 25.055959842264684 21.155916026898826 12-13 26.703244604436527 31.84734468472375 26.30204672859345 15.14736398224628 14-15 22.47295665203233 32.39818099092639 30.25662892548399 14.872233431557284 16-17 15.420585236638749 35.702341137123746 30.65703341623074 18.220040210006765 18-19 14.382249958316462 33.26632208274778 36.83967320105757 15.511754757878188 20-21 17.568713582634963 30.346652818340775 36.53337652994621 15.551257069078053 22-23 16.64438040620583 30.846568035066895 37.27263843104726 15.236413127680013 24-25 17.113437945519653 32.34633595102204 36.51294301639751 14.027283087060798 26-27 15.83986924990232 31.64590738852411 38.841689458988114 13.67253390258546 28-29 14.337130144393154 34.24847436795243 37.5029178755008 13.911477612153606 30-31 14.936543086410934 32.48946353310951 37.2469462104436 15.327047170035954 32-33 14.710050518162483 33.471319450564366 37.65615733275535 14.1624726985178 34-35 15.721653166473807 32.80761153730767 36.96862336128611 14.502111934932405 36-37 15.055545238537396 32.289092372231664 37.96408725340343 14.691275135827512 38-39 14.596071505922732 33.384821532698176 37.513476976682746 14.505629984696341 40-41 14.330631072351729 33.36800415097753 37.711535623670095 14.589829153000652 42-43 14.303328342683857 33.433622378421354 35.96361983152392 16.299429447370866 44-45 14.84168818342991 32.24444629534881 36.62744071487215 16.28642480634913 46-47 16.185881798558242 33.12929428032205 36.074691620991906 14.610132300127802 48-49 15.5822769447505 32.57143265091464 36.16715966122588 15.679130743108981 50-51 13.636666539735732 33.19085335668863 37.18703590876204 15.985444194813603 52-53 14.852408620878363 33.9253168110935 34.89704531719179 16.325229250836344 54-55 14.727267101884223 32.29431686472001 35.55535458013723 17.42306145325854 56-57 16.16700215569546 32.066802765762034 34.56441151417381 17.201783564368686 58-59 16.875229069584798 31.56781173693054 34.83642646687832 16.720532726606343 60-61 15.812581055837803 32.21982938117958 35.71263697692957 16.254952586053044 62-63 14.943404129737345 32.17753681825538 35.574479022876346 17.30458002913093 64-65 14.910913378818636 33.1306564114524 34.68297918416788 17.27545102556109 66-67 15.755569622060511 31.790274756916514 33.90699290381842 18.54716271720455 68-69 15.103300030707926 31.730094004337197 34.73304339339232 18.433562571562558 70-71 16.941524239748475 31.73147180696739 33.879954397998844 17.447049555285286 72-73 16.511960014280614 30.209210996072834 35.77293823634417 17.505890753302392 74-75 15.408852927177536 32.621846739647786 34.16682532127558 17.802475011899098 76-77 15.971229183867214 31.024514201010263 34.24945334973483 18.7548032653877 78-79 16.183472006433608 30.982671612422763 34.60419278931602 18.229663591827606 80-81 17.19927383646093 30.602606289451835 33.78992174469594 18.408198129391305 82-83 16.49381270863223 30.354011568010243 34.80895652360117 18.343219199756362 84-85 16.571854114769582 30.667161547740555 33.965496320511626 18.795488016978226 86-87 16.8650246024117 31.214607265563288 33.43215539968212 18.488212732342895 88-89 17.266135674396683 30.790965831456766 32.325622360709744 19.617276133436803 90-91 18.17211855627136 29.35256082022459 33.032616073448686 19.442704550055375 92-93 19.19508403191284 29.98554827281623 32.77589823317516 18.043469462095764 94-95 18.79582896866965 29.4150557089801 32.9528140177126 18.836301304637654 96-97 18.025551849973933 29.36058483123734 33.29881667368902 19.315046645099706 98-99 18.951945940670885 29.721206004793743 31.23549559542143 20.091352459113942 100-101 19.78380335538336 28.053872887997468 32.165325164422356 19.996998592196817 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5036.0 1 3038.5 2 1221.5 3 1365.5 4 1035.5 5 932.0 6 1719.0 7 3198.0 8 4382.0 9 4546.0 10 3408.0 11 2124.0 12 1784.0 13 1651.0 14 1511.0 15 1339.5 16 1213.5 17 1211.5 18 1248.0 19 1297.5 20 1339.0 21 1394.5 22 1466.0 23 1492.0 24 1589.5 25 1820.0 26 2157.0 27 2627.5 28 2921.5 29 3224.5 30 3770.0 31 4296.5 32 4975.5 33 5582.5 34 5961.0 35 6393.0 36 6671.5 37 6693.0 38 6291.0 39 5790.0 40 5640.0 41 5583.5 42 5291.0 43 5405.5 44 5638.5 45 5163.0 46 4916.5 47 6643.5 48 10625.0 49 10913.0 50 7003.0 51 4196.5 52 2837.5 53 2148.0 54 1653.0 55 1314.5 56 1088.0 57 887.0 58 794.5 59 727.5 60 638.5 61 559.0 62 475.5 63 421.5 64 360.0 65 313.5 66 285.5 67 243.5 68 210.0 69 191.5 70 153.0 71 122.5 72 118.0 73 115.0 74 98.5 75 71.0 76 44.5 77 30.0 78 23.5 79 14.0 80 7.5 81 5.5 82 5.0 83 4.5 84 3.5 85 3.0 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1536712197117832 2 0.04805199130306534 3 0.15271969513152447 4 0.13892258871777305 5 0.20219897330497788 6 0.2064808339161421 7 0.1655652769650172 8 0.05613995023526445 9 0.11703752337182251 10-11 0.10324041695807105 12-13 0.15105452711607173 14-15 0.14011199444309647 16-17 0.1384468264276437 18-19 0.13035886749544456 20-21 0.1408256378782905 22-23 0.19910651841913707 24-25 0.2198021780397642 26-27 0.1536712197117832 28-29 0.1313103920757033 30-31 0.0970555071863894 32-33 0.12583912573921566 34-35 0.09134635970483708 36-37 0.08563721222328476 38-39 0.05162020847903553 40-41 0.05613995023526445 42-43 0.061611216571752093 44-45 0.03877462664554282 46-47 0.046862585577741936 48-49 0.03734733977515474 50-51 0.04805199130306534 52-53 0.02283658992620927 54-55 0.04876563473825938 56-57 0.02283658992620927 58-59 0.047576229012935976 60-61 0.03401700374424922 62-63 0.049003515883324064 64-65 0.0402019135159309 66-67 0.03615793404983134 68-69 0.06922341321382185 70-71 0.05257173305929425 72-73 0.05423690107474701 74-75 0.04234284382151302 76-77 0.02164718420088587 78-79 0.02021989733049779 80-81 0.02069565962062715 82-83 0.01926837275023907 84-85 0.017841085879850994 86-87 0.022360827636079907 88-89 0.06755824519836909 90-91 0.1172754045168872 92-93 0.08539933107822008 94-95 0.0804038270318618 96-97 0.06922341321382185 98-99 0.058756642830975925 100-101 0.13678165841219092 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 210189.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 40.183358786615855 #Duplication Level Percentage of deduplicated Percentage of total 1 80.97228306555688 32.537383021946916 2 8.954428671221038 7.196380400496697 3 3.277252222919454 3.9507300572342037 4 1.62441837060892 2.6109834482299266 5 1.0063816435988207 2.021989733049779 6 0.6807875824344964 1.6413799009462913 7 0.5091107138205799 1.432044493289373 8 0.40136867903529444 1.2902673308308237 9 0.2675791193568629 0.9677004981231178 >10 2.0127632871976413 15.136377260465581 >50 0.19654041510282855 5.383726075103835 >100 0.06867074744556659 5.311410207004172 >500 0.015391719255040789 4.676267549681477 >1k 0.009471827233871254 7.574135658859407 >5k 0.0035519352127017204 8.269224364738402 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6212 2.9554353462835827 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5647 2.6866296523604944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5522 2.627159366094325 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3720 1.7698357192812184 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2969 1.412538239394069 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2101 0.9995765715617848 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1872 0.8906270071221615 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1467 0.6979432796197708 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1333 0.6341911327424365 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1288 0.6127818296866154 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1170 0.5566418794513509 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 998 0.474810765549101 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 913 0.4343709708881055 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 912 0.4338952085979761 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 879 0.41819505302370724 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 805 0.38298864355413464 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 800 0.3806098321034878 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 755 0.35920052904766664 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 749 0.35634595530689045 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 689 0.3278002178991289 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 651 0.3097212508742132 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 595 0.28307856262696907 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 579 0.2754663659848993 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 504 0.2397841942251973 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 495 0.23550233361403308 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 489 0.23264775987325692 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 467 0.22218098949041098 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 463 0.22027794032989356 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 374 0.17793509650838055 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 337 0.16033189177359425 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 310 0.14748630994010153 No Hit GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 307 0.14605902306971344 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 307 0.14605902306971344 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 295 0.14034987558816114 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 275 0.13083462978557395 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 264 0.12560124459415098 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 248 0.11798904795208122 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 244 0.11608599879156377 No Hit CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 233 0.11085261360014084 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 231 0.1099010890198821 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 226 0.1075222775692353 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 216 0.10276465466794171 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 214 0.101813130087683 No Hit GTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA 212 0.10086160550742426 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.757622901293598E-4 0.0 2 0.0 0.0 0.0 4.757622901293598E-4 0.0 3 0.0 0.0 0.0 4.757622901293598E-4 0.0 4 0.0 0.0 0.0 4.757622901293598E-4 0.0 5 0.0 0.0 0.0 4.757622901293598E-4 0.0 6 0.0 0.0 0.0 4.757622901293598E-4 4.757622901293598E-4 7 0.0 0.0 0.0 4.757622901293598E-4 4.757622901293598E-4 8 0.0 0.0 0.0 4.757622901293598E-4 4.757622901293598E-4 9 0.0 0.0 0.0 4.757622901293598E-4 4.757622901293598E-4 10-11 0.0 0.0 0.0 4.757622901293598E-4 4.757622901293598E-4 12-13 0.0 0.0 0.0 4.757622901293598E-4 4.757622901293598E-4 14-15 0.0 0.0 0.0 4.757622901293598E-4 4.757622901293598E-4 16-17 0.0 0.0 0.0 4.757622901293598E-4 4.757622901293598E-4 18-19 0.0 0.0 0.0 9.515245802587196E-4 4.757622901293598E-4 20-21 0.0 0.0 0.0 0.0028545737407761583 4.757622901293598E-4 22-23 0.0 0.0 0.0 0.010466770382845915 4.757622901293598E-4 24-25 0.0 0.0 0.0 0.029021499697890946 4.757622901293598E-4 26-27 0.0 0.0 0.0 0.059232405121105294 4.757622901293598E-4 28-29 0.0 0.0 0.0 0.13321344123622073 4.757622901293598E-4 30-31 0.0 0.0 0.0 0.2780830585806108 4.757622901293598E-4 32-33 0.0 0.0 0.0 0.5021670972315392 4.757622901293598E-4 34-35 0.0 0.0 0.0 0.8038003891735533 4.757622901293598E-4 36-37 0.0 0.0 0.0 1.1998724957062454 4.757622901293598E-4 38-39 0.0 0.0 0.0 1.666119540033018 4.757622901293598E-4 40-41 0.0 0.0 0.0 2.123089219702268 4.757622901293598E-4 42-43 0.0 0.0 0.0 2.564358743797249 4.757622901293598E-4 44-45 0.0 0.0 0.0 2.9949236163643196 4.757622901293598E-4 46-47 0.0 0.0 0.0 3.469496500768356 4.757622901293598E-4 48-49 0.0 0.0 0.0 3.9076735699774963 4.757622901293598E-4 50-51 0.0 0.0 0.0 4.295895598723054 4.757622901293598E-4 52-53 0.0 0.0 0.0 4.6598537506720135 4.757622901293598E-4 54-55 0.0 0.0 0.0 5.067344152167811 4.757622901293598E-4 56-57 0.0 0.0 0.0 5.575696159171033 4.757622901293598E-4 58-59 0.0 0.0 0.0 6.028859740519247 4.757622901293598E-4 60-61 0.0 0.0 0.0 6.480596034997074 4.757622901293598E-4 62-63 0.0 0.0 0.0 6.9018835429066225 4.757622901293598E-4 64-65 0.0 0.0 0.0 7.321505882800718 4.757622901293598E-4 66-67 0.0 0.0 0.0 7.805118250717212 4.757622901293598E-4 68-69 0.0 0.0 0.0 8.337972015662094 4.757622901293598E-4 70-71 0.0 0.0 0.0 8.882481956715147 4.757622901293598E-4 72-73 0.0 0.0 0.0 9.409626574178478 4.757622901293598E-4 74-75 0.0 0.0 0.0 9.940577289962842 4.757622901293598E-4 76-77 2.378811450646799E-4 0.0 0.0 10.456779374753198 4.757622901293598E-4 78-79 4.757622901293598E-4 0.0 0.0 11.088115933754858 4.757622901293598E-4 80-81 4.757622901293598E-4 0.0 0.0 11.707796316648349 4.757622901293598E-4 82-83 4.757622901293598E-4 0.0 0.0 12.229469667775193 4.757622901293598E-4 84-85 4.757622901293598E-4 0.0 0.0 12.777785707149281 4.757622901293598E-4 86-87 4.757622901293598E-4 0.0 0.0 13.332524537440113 4.757622901293598E-4 88-89 4.757622901293598E-4 0.0 0.0 13.923421301780778 4.757622901293598E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACATGCC 25 0.0049884315 57.078167 3 CCGCTCA 20 5.3858454E-4 47.49714 88-89 GTAAGCG 70 0.0 40.72639 94-95 GTCTAGA 50 0.0015907392 38.106667 1 TAGGGTC 85 1.6106897E-5 33.57539 4 AGGGTCT 95 3.4450037E-5 30.041142 5 CACCTAC 80 4.4703472E-4 29.699865 7 GTCCAAC 65 0.0057539395 29.312819 1 ACCTACT 85 6.374055E-4 27.952814 8 CATAGGG 105 6.815487E-5 27.18008 2 TGTACGA 35 0.008329727 27.180079 18-19 CCCAGCA 70 0.008359215 27.154161 9 GGTCTTC 105 6.859924E-5 27.154161 7 AGAGCAG 95 0.0012134765 25.034285 5 GTCTTCT 115 1.2732724E-4 24.792933 8 GAGTACT 3120 0.0 23.75423 12-13 ATGGGGG 245 2.8740033E-10 23.29721 5 TCTACAC 165 2.385581E-6 23.061888 3 GTACTTT 3140 0.0 23.003206 14-15 CATGGGG 995 0.0 22.467955 4 >>END_MODULE