##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938040_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 210189 Sequences flagged as poor quality 0 Sequence length 101 %GC 34 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.367198093144744 33.0 31.0 34.0 31.0 34.0 2 32.55466746594731 34.0 31.0 34.0 31.0 34.0 3 32.545799256859304 34.0 31.0 34.0 31.0 34.0 4 36.06025053642198 37.0 35.0 37.0 35.0 37.0 5 36.071145492865945 37.0 35.0 37.0 35.0 37.0 6 36.17381499507586 37.0 36.0 37.0 35.0 37.0 7 36.128750790954804 37.0 36.0 37.0 35.0 37.0 8 36.156987282873985 37.0 37.0 37.0 35.0 37.0 9 37.742612601040015 39.0 38.0 39.0 35.0 39.0 10-11 37.7695669135873 39.0 38.0 39.0 35.0 39.0 12-13 37.70570058376033 39.0 38.0 39.0 35.0 39.0 14-15 38.761145921052005 40.0 38.0 41.0 34.5 41.0 16-17 38.67405763384383 40.0 38.0 41.0 34.5 41.0 18-19 38.95453139793234 40.0 38.0 41.0 35.0 41.0 20-21 39.00984827940568 40.0 38.5 41.0 35.0 41.0 22-23 38.863263539005374 40.0 38.0 41.0 35.0 41.0 24-25 38.67429551498889 40.0 38.0 41.0 35.0 41.0 26-27 38.37245526645067 40.0 37.5 41.0 34.5 41.0 28-29 38.12155250750515 40.0 37.0 41.0 34.0 41.0 30-31 37.68062077463616 39.5 35.5 41.0 33.5 41.0 32-33 37.145071816317696 39.0 35.0 41.0 33.0 41.0 34-35 36.75681410540038 39.0 35.0 41.0 33.0 41.0 36-37 36.50298302955911 38.5 35.0 41.0 32.5 41.0 38-39 36.17139574383055 38.0 35.0 41.0 31.0 41.0 40-41 35.954402942114 38.0 35.0 41.0 31.0 41.0 42-43 35.54075141896104 38.0 35.0 41.0 30.5 41.0 44-45 35.034547478697746 38.0 35.0 40.5 27.5 41.0 46-47 34.335134093601475 38.0 35.0 40.0 23.5 41.0 48-49 33.657237058076305 37.0 34.0 40.0 17.5 41.0 50-51 33.1375571509451 37.0 33.0 40.0 9.5 41.0 52-53 32.69825252510836 36.5 33.0 40.0 3.5 41.0 54-55 32.390607976630555 36.0 33.0 40.0 2.0 41.0 56-57 31.93387379929492 36.0 33.0 40.0 2.0 41.0 58-59 31.403001584288425 35.0 31.5 40.0 2.0 41.0 60-61 30.788540313717654 35.0 31.0 39.0 2.0 41.0 62-63 30.287588789137395 35.0 30.0 39.0 2.0 40.0 64-65 29.737179395686738 35.0 29.0 38.5 2.0 40.0 66-67 29.081291123702954 35.0 28.0 38.0 2.0 40.0 68-69 28.46539542982744 35.0 26.0 37.0 2.0 39.5 70-71 27.93976135763527 34.5 26.0 36.5 2.0 39.0 72-73 27.425252986597776 34.0 25.0 36.0 2.0 39.0 74-75 26.949766638596692 34.0 24.0 35.5 2.0 38.0 76-77 25.61133075470172 32.0 21.0 34.5 2.0 36.0 78-79 26.267428362093163 33.0 21.5 35.0 2.0 37.0 80-81 25.971035591776925 33.0 20.0 35.0 2.0 36.5 82-83 25.520388792943493 33.0 17.0 35.0 2.0 36.0 84-85 25.033557893134272 33.0 9.5 35.0 2.0 35.5 86-87 24.56089995194801 32.5 4.0 35.0 2.0 35.0 88-89 23.943317680753985 32.0 2.0 35.0 2.0 35.0 90-91 23.51381137928246 31.5 2.0 35.0 2.0 35.0 92-93 22.880193540099626 31.0 2.0 34.0 2.0 35.0 94-95 22.25126195947457 31.0 2.0 34.0 2.0 35.0 96-97 21.41070417576562 30.0 2.0 34.0 2.0 35.0 98-99 20.317709299725486 29.0 2.0 34.0 2.0 35.0 100-101 16.70452307209226 20.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.18344194917209933 1101 2 0.21854308176117598 1101 3 0.14569997697574166 1101 4 0.1408289391404125 1101 5 0.19417201914940563 1101 6 0.1253195564986811 1101 7 -0.012224165088795758 1101 8 0.050943667225894274 1101 9 -0.1553241497770017 1101 10-11 -0.10500872828124841 1101 12-13 0.27058483085847485 1101 14-15 0.5997199121741517 1101 16-17 0.2323918042798283 1101 18-19 0.30680007467569936 1101 20-21 0.36228628281572384 1101 22-23 0.172588423944795 1101 24-25 0.30767885648099735 1101 26-27 0.42369287411622025 1101 28-29 0.45779570491992416 1101 30-31 0.47464975507387663 1101 32-33 0.7055192429493076 1101 34-35 0.5364089942110084 1101 36-37 0.48278989676166617 1101 38-39 0.6407823726752753 1101 40-41 0.8122569271737561 1101 42-43 0.8628441604275991 1101 44-45 1.031731386638853 1101 46-47 1.0207754481493794 1101 48-49 0.49553662037046564 1101 50-51 0.8041035782335868 1101 52-53 0.5521169246690576 1101 54-55 0.6643823194765872 1101 56-57 0.538136799397094 1101 58-59 0.6596480256310855 1101 60-61 0.6662033203003723 1101 62-63 0.521313424317281 1101 64-65 0.3476561184236786 1101 66-67 0.05751985421392547 1101 68-69 0.04473993424047151 1101 70-71 0.10200903097380731 1101 72-73 0.2142488444993056 1101 74-75 0.16604391608625235 1101 76-77 0.9028782242751809 1101 78-79 0.5315286086481485 1101 80-81 0.9256334121940455 1101 82-83 1.0969882545281102 1101 84-85 0.7818260066969103 1101 86-87 1.1310807150662718 1101 88-89 0.6974009851892298 1101 90-91 0.45477187718332246 1101 92-93 0.5580397111431594 1101 94-95 0.5282729295293684 1101 96-97 0.8434553894027879 1101 98-99 0.4614644400257184 1101 100-101 -0.4046711586370755 1102 1 -0.023395657665510328 1102 2 0.12794479116288215 1102 3 0.11924495052070938 1102 4 0.15499255330402661 1102 5 0.17610120107858762 1102 6 0.14648357766270692 1102 7 0.2197660669014354 1102 8 -0.022072205789982036 1102 9 -0.03509622954908309 1102 10-11 0.04635472308220301 1102 12-13 0.12821608848973653 1102 14-15 0.2735904860447249 1102 16-17 0.37170804359607246 1102 18-19 0.04481391268954127 1102 20-21 -0.03596361543417714 1102 22-23 0.023789075145437266 1102 24-25 -0.03700978820764789 1102 26-27 0.15519470561805093 1102 28-29 0.12377047089469073 1102 30-31 0.03940351982763701 1102 32-33 0.13148586891593794 1102 34-35 0.09172034952236174 1102 36-37 -0.017128703156927827 1102 38-39 0.23756706945997053 1102 40-41 0.11209412701095545 1102 42-43 0.16398376156720218 1102 44-45 -0.015602760695294648 1102 46-47 0.23363666101058556 1102 48-49 0.8199566447904871 1102 50-51 0.1910388651688706 1102 52-53 0.1678271403792735 1102 54-55 0.24639290148716242 1102 56-57 0.20390806516835625 1102 58-59 0.3183374843205442 1102 60-61 0.2933502474473002 1102 62-63 0.33844814145199464 1102 64-65 -0.17867680790924823 1102 66-67 -0.38277318607911326 1102 68-69 -0.06832477882424115 1102 70-71 0.03717386613864093 1102 72-73 0.15014628039673994 1102 74-75 -0.15805050800817 1102 76-77 -0.21588449448753622 1102 78-79 -0.11454383742429641 1102 80-81 0.09400948057011504 1102 82-83 -0.008384150844293004 1102 84-85 -0.42916300429209997 1102 86-87 -0.5979791139935564 1102 88-89 -0.2959649081766642 1102 90-91 -0.7352973128858693 1102 92-93 -0.7250290719256256 1102 94-95 -0.6777099764535386 1102 96-97 -1.0770981311507377 1102 98-99 -0.28717210861082876 1102 100-101 -0.8856235395894547 1103 1 0.0921721079022575 1103 2 0.22137754888135674 1103 3 0.25870073283363837 1103 4 0.13345932462793542 1103 5 0.2871425406913559 1103 6 0.1192726933089645 1103 7 0.17201619772299637 1103 8 0.18132915475423772 1103 9 0.211266931099793 1103 10-11 0.2947456179016683 1103 12-13 0.18526737054101972 1103 14-15 5.135629678036935E-4 1103 16-17 0.231258017431756 1103 18-19 0.04990287134992144 1103 20-21 0.3545387393538917 1103 22-23 0.47096332946255615 1103 24-25 0.41397137348780433 1103 26-27 0.665189472755678 1103 28-29 0.5713633542018641 1103 30-31 0.09190221161204448 1103 32-33 0.20674751203471686 1103 34-35 0.14865389217019498 1103 36-37 0.36043539940717295 1103 38-39 0.16128501817792085 1103 40-41 0.23383667018206467 1103 42-43 0.5070240546074984 1103 44-45 0.6421811220885942 1103 46-47 -0.03742561005167744 1103 48-49 -0.27713911659098756 1103 50-51 -0.43597578684578053 1103 52-53 -0.26298133685777714 1103 54-55 0.023603785840904834 1103 56-57 -0.3225963827646652 1103 58-59 -0.7543731383900791 1103 60-61 -0.4829841324585118 1103 62-63 -0.1905157517976086 1103 64-65 -0.43163337515153444 1103 66-67 -0.4807454519085219 1103 68-69 0.05466056916111128 1103 70-71 -0.1718004928357182 1103 72-73 -0.3529280262489962 1103 74-75 -0.18505207786688516 1103 76-77 0.08815789169770838 1103 78-79 0.06308567591950265 1103 80-81 -0.01626001184223469 1103 82-83 0.3356503860473836 1103 84-85 0.521621925064256 1103 86-87 -0.24873787903803546 1103 88-89 -0.2791674199506069 1103 90-91 -0.43081013339869045 1103 92-93 -0.6748590038984119 1103 94-95 -0.6729742360035118 1103 96-97 -0.9920510353893555 1103 98-99 0.3195521195419708 1103 100-101 -1.4361337436710873 1104 1 -0.04043043806662894 1104 2 -0.16814558710571248 1104 3 0.0911921452401856 1104 4 0.008737158533776324 1104 5 0.004560200824720084 1104 6 -0.24130520121518373 1104 7 -0.015445915835300639 1104 8 -0.026692667836186956 1104 9 0.09366741604624451 1104 10-11 0.03790080076689151 1104 12-13 -0.038742530449077606 1104 14-15 -0.11334782317080538 1104 16-17 -0.2332297578763871 1104 18-19 -0.19537395225080445 1104 20-21 0.19061673490854503 1104 22-23 -0.07510658365120548 1104 24-25 0.1622285971693529 1104 26-27 0.29486213389934335 1104 28-29 0.11930233177309191 1104 30-31 -0.05946675181490235 1104 32-33 0.009005543960363127 1104 34-35 0.06752639166504082 1104 36-37 0.45573242911014944 1104 38-39 0.5882652161977262 1104 40-41 1.047890014291987 1104 42-43 0.36770297964285703 1104 44-45 0.18974123524276365 1104 46-47 -0.16932094244206297 1104 48-49 -0.4063571404341957 1104 50-51 -0.44145669207915716 1104 52-53 -1.0849908337258292 1104 54-55 -0.6455212899319775 1104 56-57 -1.4315577891093767 1104 58-59 -1.1482911016149728 1104 60-61 -1.276604055675321 1104 62-63 -1.3632274107186042 1104 64-65 -1.1517283438320725 1104 66-67 -1.671970952009552 1104 68-69 -1.5869589499336563 1104 70-71 -1.2510084136277975 1104 72-73 -1.3855861389990451 1104 74-75 -1.2399621607119045 1104 76-77 -1.3290223467342983 1104 78-79 -1.2050998160990893 1104 80-81 -1.0163357847256442 1104 82-83 -1.4191990784909052 1104 84-85 -1.1941287316340663 1104 86-87 -1.0798653795431896 1104 88-89 -1.5655384039877838 1104 90-91 -1.1028398363689895 1104 92-93 -1.1293195009685277 1104 94-95 -1.1603959373573218 1104 96-97 -1.2717287480585817 1104 98-99 -1.5721936876918114 1104 100-101 -1.4638413613716335 1105 1 0.1278863936165422 1105 2 0.0927260882971197 1105 3 0.09473514659914173 1105 4 -0.031206541718603376 1105 5 0.02843905929879753 1105 6 0.2494247541332939 1105 7 0.08137994322119368 1105 8 0.011261127543349403 1105 9 0.25208325763040307 1105 10-11 0.33391128416817395 1105 12-13 0.10928697838415502 1105 14-15 0.2872782688501516 1105 16-17 0.16213036637132916 1105 18-19 0.16234784934112412 1105 20-21 -0.039168743639308445 1105 22-23 -0.10211590223011768 1105 24-25 0.06363123743338406 1105 26-27 0.18279651557279664 1105 28-29 -0.02547538129233118 1105 30-31 0.054637305649663404 1105 32-33 0.5102189005901394 1105 34-35 0.33647902222221404 1105 36-37 0.27710206607384436 1105 38-39 -0.19410713868482787 1105 40-41 -0.452837999685876 1105 42-43 -0.2496286064569304 1105 44-45 -0.1267264409366149 1105 46-47 -0.5538821926847319 1105 48-49 -0.3480674879395309 1105 50-51 -0.005303517938209268 1105 52-53 -0.03288930004305257 1105 54-55 0.1900203524675561 1105 56-57 -0.3635127493112833 1105 58-59 -0.9795131944124904 1105 60-61 -0.6034823317382205 1105 62-63 -0.2992592491965702 1105 64-65 0.6290909145643582 1105 66-67 -0.2618078768785139 1105 68-69 0.4191563674804364 1105 70-71 0.6247681346152625 1105 72-73 0.3515037464600894 1105 74-75 0.6158182702723707 1105 76-77 0.33965849193780073 1105 78-79 -0.33102196713771903 1105 80-81 -0.27371299303471375 1105 82-83 -0.3619286455652606 1105 84-85 -0.2934541053479087 1105 86-87 -0.2496382391820937 1105 88-89 -0.6531670197905406 1105 90-91 -1.0484571922222194 1105 92-93 -1.3598329934942512 1105 94-95 -0.908818573738607 1105 96-97 -0.4544359893709462 1105 98-99 -0.21726260634838468 1105 100-101 -0.5209176572365131 1106 1 0.3167752825054322 1106 2 0.45743197065006314 1106 3 0.5525782838540465 1106 4 0.3011463994578705 1106 5 0.35687043184781686 1106 6 0.24092802210714837 1106 7 0.09968059681596486 1106 8 0.061261127543353666 1106 9 0.3465277020748445 1106 10-11 0.31430344103091556 1106 12-13 0.3297118149854654 1106 14-15 0.2671802296344694 1106 16-17 0.4212806931687254 1106 18-19 0.10058314345877051 1106 20-21 0.25805347858292293 1106 22-23 0.3254984768548397 1106 24-25 0.2219645707667155 1106 26-27 0.21416906459240437 1106 28-29 0.318642265766492 1106 30-31 0.7597026651267882 1106 32-33 0.5299901424202105 1106 34-35 0.6729169307189409 1106 36-37 0.8104353994071758 1106 38-39 0.49184057373347656 1106 40-41 0.5625214774383025 1106 42-43 0.49988119746463866 1106 44-45 0.6294827093901816 1106 46-47 0.5415426439165785 1106 48-49 0.21418741402125363 1106 50-51 -0.23438848525847078 1106 52-53 0.05158782413995766 1106 54-55 -0.03138487629061615 1106 56-57 0.08959182585211778 1106 58-59 0.4337220997051574 1106 60-61 -0.14318821409116111 1106 62-63 -0.2551416021377477 1106 64-65 -0.17483065406309706 1106 66-67 -0.19089284419877117 1106 68-69 -0.2576410181404789 1106 70-71 -0.9718004928357153 1106 72-73 -0.7079733777229187 1106 74-75 -0.6448026447603077 1106 76-77 -0.7069101355131799 1106 78-79 -0.018603666484121817 1106 80-81 0.16730007886071263 1106 82-83 -0.5720593645195073 1106 84-85 -0.19198351711261452 1106 86-87 -0.24669706271150815 1106 88-89 -0.39810166031341865 1106 90-91 -0.7585879111764662 1106 92-93 -0.31947351637006705 1106 94-95 0.04493959619603416 1106 96-97 -0.5245340285866327 1106 98-99 -0.1636678351065548 1106 100-101 -0.9050679840338987 1107 1 0.2270053363478226 1107 2 0.10155825552524789 1107 3 0.13099238077034414 1107 4 0.026990746007065525 1107 5 0.015093633022559061 1107 6 0.11270971569501143 1107 7 0.09867717048214075 1107 8 0.3467971040484912 1107 9 0.2256515395687302 1107 10-11 0.3433049094597038 1107 12-13 0.202337855122515 1107 14-15 -0.020411547164357557 1107 16-17 0.17610203433367388 1107 18-19 0.18542161629283527 1107 20-21 0.026726997917229767 1107 22-23 0.073026621739821 1107 24-25 0.39054019484894553 1107 26-27 0.4005126769272138 1107 28-29 0.17576414535533047 1107 30-31 0.08465371750074979 1107 32-33 0.052371444427059544 1107 34-35 0.5348356776597214 1107 36-37 0.4810301130635608 1107 38-39 0.6563290710413554 1107 40-41 0.8568925004436849 1107 42-43 0.6179428714734456 1107 44-45 0.5377915689104853 1107 46-47 0.8543302112195548 1107 48-49 1.0623151673252238 1107 50-51 0.6265635460680059 1107 52-53 0.5883964389221461 1107 54-55 0.9739993625738066 1107 56-57 1.2645428782260808 1107 58-59 1.0163946400869506 1107 60-61 1.2837941647634636 1107 62-63 1.190159425762399 1107 64-65 1.393818391458197 1107 66-67 1.5666450902114093 1107 68-69 1.2152175232202644 1107 70-71 0.7786400357986416 1107 72-73 0.9132209443524602 1107 74-75 0.5584523723713595 1107 76-77 0.5438240788774245 1107 78-79 0.7537042140836654 1107 80-81 0.9789863245777966 1107 82-83 1.180094331026261 1107 84-85 0.8916435019769615 1107 86-87 0.6748888403721942 1107 88-89 0.8994264845715527 1107 90-91 0.44418986660130955 1107 92-93 0.42107959180418675 1107 94-95 0.13375506364586442 1107 96-97 0.3366260301505193 1107 98-99 -0.1864040074021993 1107 100-101 -0.22394953077888502 1108 1 -0.10100249527234695 1108 2 -0.017012473794380867 1108 3 0.05702272829849164 1108 4 0.13447973279120617 1108 5 -0.09979623481884659 1108 6 0.09315024432937236 1108 7 0.1585694857048523 1108 8 0.03126112754335253 1108 9 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1.553303241604116 2316 72-73 1.6056734641396915 2316 74-75 1.7757828785223104 2316 76-77 1.6772530733388287 2316 78-79 1.8917121472549248 2316 80-81 1.665836270654168 2316 82-83 1.6506008475597902 2316 84-85 1.8147246433299848 2316 86-87 1.9611867547158788 2316 88-89 2.025388427284554 2316 90-91 1.921368289837826 2316 92-93 1.089632062237584 2316 94-95 1.717300131004702 2316 96-97 2.3618421816484982 2316 98-99 2.54778905747067 2316 100-101 2.69362957244374 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 4.0 5 13.0 6 35.0 7 61.0 8 170.0 9 284.0 10 505.0 11 788.0 12 1038.0 13 1523.0 14 1905.0 15 2680.0 16 3484.0 17 4506.0 18 4706.0 19 3416.0 20 3111.0 21 3208.0 22 3501.0 23 3476.0 24 3763.0 25 3611.0 26 3237.0 27 3507.0 28 4007.0 29 4729.0 30 5485.0 31 6619.0 32 7929.0 33 10152.0 34 14865.0 35 25895.0 36 24633.0 37 33140.0 38 19303.0 39 898.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.79692050018081 23.304181496355227 16.20353628594812 15.695361717515846 2 15.286521157790053 26.25964150952374 38.25722429208361 20.19661304060259 3 20.21934624351715 31.25469857734215 33.86591806632726 14.660037112813438 4 10.599508061792006 23.261921413584915 37.42821936447673 28.710351160146345 5 8.118600844973928 43.73881932021467 34.45267004148746 13.689909793323945 6 21.776530199586077 41.48535813687941 17.23148654756524 19.506625115969264 7 19.42299549453111 42.39327462426673 21.28227452435665 16.901455356845503 8 24.0978357573422 32.7624185851781 20.691853522306115 22.447892135173582 9 24.198221600559496 20.044341045440056 23.09588037432977 32.66155697967068 10-11 22.90176935995699 30.264190799708835 25.020100956757968 21.81393888357621 12-13 26.3668715626011 31.344082440481852 26.101870706224904 16.187175290692142 14-15 22.254494764235996 32.19055231244261 29.318375366931665 16.236577556389726 16-17 15.538417255234066 36.01413989071558 29.91619307706764 18.531249776982715 18-19 14.532397033146358 32.954626550390365 35.77518328742227 16.737793129041005 20-21 17.283968238109512 30.331749044907202 35.607952842441804 16.776329874541485 22-23 16.68522139598171 31.45930567251379 36.15579311952576 15.699679811978742 24-25 17.361112763138177 32.8523952145666 35.685925601688076 14.100566420607146 26-27 16.1808478492078 32.1347079495678 37.94930606942399 13.735138131800412 28-29 14.050092067011462 34.0833503827799 36.9217263873095 14.944831162899137 30-31 15.29974307736226 32.82091540584261 36.58840041868874 15.290941098106384 32-33 15.42611649515436 33.13612986407472 37.55310696563569 13.884646675135235 34-35 15.001189513251179 32.7337393538564 37.46752628824285 14.797544844649568 36-37 15.036646026267148 32.43024647158595 37.67154408108989 14.861563421057014 38-39 14.274839305170307 33.116694658413465 37.581180030545106 15.02728600587113 40-41 13.876921832589142 33.237861202235194 37.95873605682654 14.926480908349133 42-43 14.088196041954614 33.26870603460376 36.43480929396064 16.20828862948099 44-45 14.293802244646484 32.26167877481695 36.88989433319536 16.5546246473412 46-47 16.224522808152702 32.70215235884066 35.761080556454225 15.31224427655242 48-49 15.311933545523315 31.990018507153085 36.298759687709634 16.399288259613968 50-51 13.027611981130505 32.95381826572874 37.65049564540954 16.36807410773121 52-53 14.30172169738379 33.880587427826455 35.59038970740423 16.227301167385527 54-55 14.003977618757613 32.45422883678441 35.61657848660171 17.925215057856274 56-57 16.004749056612752 32.5191413629507 33.85511094614722 17.620998634289332 58-59 15.999723974481917 32.89248568117625 34.6285910087067 16.479199335635133 60-61 15.290336547268154 34.23851630875661 34.3274883016103 16.143658842364943 62-63 13.869172399188127 34.86364490806947 34.00680050349424 17.260382189248162 64-65 13.807372085442141 35.563138145105064 33.19223054244862 17.43725922700417 66-67 14.689227899540336 33.88324752324486 32.43526176042331 18.9922628167915 68-69 14.011712717174287 33.458583099134515 33.405516542249785 19.124187641441413 70-71 15.96220785678767 32.92989038755743 32.43191156771727 18.675990187937636 72-73 15.49682769645801 31.18396981613248 34.37934898193678 18.93985350547273 74-75 14.489446798588624 33.17876083454874 32.68030616299349 19.65148620386915 76-77 14.806874611857587 31.448755683832573 33.57857192145068 20.165797782859155 78-79 14.959380160766042 31.136165696581465 33.98478957162369 19.919664571028797 80-81 15.829193551457013 30.72836369173376 33.098031938179254 20.34441081862997 82-83 14.98638769371359 30.31236911243955 34.34204013250581 20.35920306134105 84-85 14.969900302186689 30.56130582720632 33.65360363575797 20.815190234849027 86-87 15.162425664468627 31.092063023680282 33.18666517539497 20.55884613645612 88-89 15.104198889243174 30.628143932003027 31.954008766127153 22.313648412626648 90-91 15.88109170607035 29.079798107195465 32.78140415922022 22.25770602751396 92-93 16.653103182421724 29.715452377892948 32.78865236076013 20.8427920789252 94-95 16.034481117709763 28.82975483477996 33.200091365920514 21.935672681589764 96-97 15.113338139527139 29.263534075200276 33.60957051185964 22.013557273412946 98-99 15.685364814933775 29.42121153722618 31.762584194009648 23.130839453830397 100-101 15.529067485100462 28.21122253547934 32.74972342171942 23.50998655770077 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4543.0 1 2708.5 2 1080.0 3 1244.5 4 901.5 5 874.5 6 1787.5 7 3308.5 8 4462.5 9 4518.5 10 3376.0 11 2157.0 12 1805.5 13 1699.0 14 1550.0 15 1353.0 16 1187.5 17 1115.5 18 1145.5 19 1170.0 20 1197.0 21 1233.0 22 1290.0 23 1358.0 24 1468.0 25 1659.5 26 1974.5 27 2437.5 28 2657.0 29 2946.0 30 3567.5 31 4038.0 32 4776.5 33 5483.0 34 5996.0 35 6509.5 36 6773.0 37 6836.0 38 6445.5 39 6051.5 40 5861.0 41 5785.0 42 5423.5 43 5576.5 44 6020.5 45 5535.5 46 5157.0 47 7223.0 48 11545.0 49 10940.5 50 6676.0 51 4710.5 52 4076.0 53 3426.5 54 2186.0 55 1241.5 56 874.0 57 668.0 58 555.5 59 500.5 60 407.0 61 327.5 62 244.0 63 169.0 64 122.0 65 97.0 66 75.0 67 60.5 68 51.0 69 32.5 70 18.5 71 22.0 72 28.0 73 41.5 74 43.0 75 26.5 76 13.0 77 6.0 78 4.5 79 2.5 80 0.5 81 0.0 82 0.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.011894057253233994 2 0.014272868703880793 3 0.009039483512457836 4 0.0 5 0.0023788114506467987 6 0.0019030491605174392 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 4.757622901293598E-4 14-15 0.0 16-17 0.0016651680154527592 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004519741756228918 26-27 0.02164718420088587 28-29 0.007612196642069757 30-31 0.0042818606111642375 32-33 0.0 34-35 0.009039483512457836 36-37 0.0011894057253233994 38-39 0.0038060983210348783 40-41 0.0026166925957114787 42-43 0.004519741756228918 44-45 0.0 46-47 4.757622901293598E-4 48-49 0.0 50-51 0.004043979466099558 52-53 0.008801602367393156 54-55 0.0061849097716816775 56-57 0.019982016185433108 58-59 0.030210905423214345 60-61 0.004995504046358277 62-63 0.027356331682438187 64-65 0.027832093972567546 66-67 0.01665168015452759 68-69 0.035920052904766665 70-71 0.018792610460109712 72-73 0.004995504046358277 74-75 0.018792610460109712 76-77 0.02545328252192075 78-79 0.03330336030905518 80-81 0.015700155574268872 82-83 0.04043979466099558 84-85 0.025691163666985428 86-87 0.036871577485025384 88-89 0.029497261988020305 90-91 0.036871577485025384 92-93 0.031400311148537745 94-95 0.02140930305582119 96-97 0.02069565962062715 98-99 0.016413799009462913 100-101 0.014986512139074832 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 210189.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.007593166150464 #Duplication Level Percentage of deduplicated Percentage of total 1 78.27017599492628 28.183206542683013 2 9.99550763701707 7.198283449657213 3 3.8356852174832192 4.143413784736595 4 1.8854711695999153 2.7156511520583857 5 1.14951641033772 2.0695659620627147 6 0.8442999841446013 1.8240726203559654 7 0.5787220548596798 1.458687181536617 8 0.41091908461497806 1.1836965778418471 9 0.39770625231224566 1.2888400439604355 >10 2.2963902542148933 15.819571909091342 >50 0.20479890069235243 5.133475110495792 >100 0.0924898261191269 6.250089205429399 >500 0.021140531684371862 5.5511944012293695 >1k 0.014534115533005654 11.62858189534181 >5k 0.0026425664605464827 5.551670163519499 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6063 2.8845467650543086 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5606 2.6671233984651908 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4985 2.3716750162948586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3815 1.8150331368435075 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3007 1.4306172064189848 No Hit CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT 2108 1.0029069075926904 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2064 0.9819733668269985 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1913 0.9101332610174653 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1599 0.7607439019168463 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1402 0.6670187307613624 No Hit ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT 1246 0.5927998135011823 TruSeq Adapter, Index 10 (95% over 21bp) GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1225 0.5828088054084657 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1078 0.5128717487594499 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 929 0.4419831675301752 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 914 0.4348467331782348 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 899 0.4277102988262944 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 877 0.4172435284434485 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 844 0.4015433728691797 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 832 0.39583422538762736 No Hit ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT 751 0.3572974798871492 RNA PCR Primer, Index 10 (95% over 23bp) GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 745 0.354442906146373 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 744 0.35396714385624367 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 740 0.35206409469572625 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 622 0.2959241444604618 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 602 0.28640889865787456 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 599 0.2849816117874865 No Hit TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTG 563 0.2678541693428296 TruSeq Adapter, Index 10 (96% over 28bp) CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 504 0.2397841942251973 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 503 0.23930843193506798 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 472 0.2245598009410578 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 461 0.21932641574963482 No Hit GTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 455 0.21647184200885872 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 441 0.20981116994704765 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 374 0.17793509650838055 No Hit CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCT 351 0.16699256383540528 TruSeq Adapter, Index 10 (96% over 26bp) GTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 343 0.1631864655143704 No Hit GAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 310 0.14748630994010153 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 307 0.14605902306971344 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 306 0.14558326077958408 No Hit AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 291 0.1384468264276437 No Hit TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC 277 0.13178615436583266 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 259 0.12322243314350419 No Hit TTTGTAAGGTGGCTCTATTTCTCTTGTCCTTTCGTACTGGGAGAAATCGT 257 0.12227090856324546 No Hit TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 256 0.1217951462731161 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 247 0.11751328566195185 No Hit GAGTACATGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 244 0.11608599879156377 No Hit CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 229 0.10894956443962339 No Hit CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG 229 0.10894956443962339 TruSeq Adapter, Index 10 (95% over 24bp) CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 221 0.1051434661185885 No Hit GGTATCAACGCAGAGTACATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAA 216 0.10276465466794171 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 211 0.10038584321729492 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0019030491605174392 0.0 22-23 0.0 0.0 0.0 0.009515245802587195 0.0 24-25 0.0 0.0 0.0 0.028545737407761583 0.0 26-27 0.0 0.0 0.0 0.05851876168591125 0.0 28-29 0.0 0.0 0.0 0.13440284696154414 0.0 30-31 0.0 0.0 0.0 0.27951034545099884 0.0 32-33 0.0 0.0 0.0 0.5057353144075094 0.0 34-35 0.0 0.0 0.0 0.8128398726860112 0.0 36-37 0.0 0.0 0.0 1.2131938398298674 0.0 38-39 0.0 0.0 0.0 1.6851500316381922 0.0 40-41 0.0 0.0 0.0 2.145212166193283 0.0 42-43 0.0 0.0 0.0 2.5848165222728117 0.0 44-45 0.0 0.0 0.0 3.0196632554510465 0.0 46-47 0.0 0.0 0.0 3.498042238176118 0.0 48-49 0.0 0.0 0.0 3.940025405706293 0.0 50-51 0.0 0.0 0.0 4.333718700788339 0.0 52-53 0.0 0.0 0.0 4.703386000218851 0.0 54-55 0.0 0.0 0.0 5.11610978690607 0.0 56-57 0.0 0.0 0.0 5.628743654520456 0.0 58-59 0.0 0.0 0.0 6.083096641593993 0.0 60-61 0.0 0.0 0.0 6.53578446065208 0.0 62-63 0.0 0.0 0.0 6.961353829172792 0.0 64-65 0.0 0.0 0.0 7.380500406776758 0.0 66-67 0.0 0.0 0.0 7.866253704998834 0.0 68-69 0.0 0.0 0.0 8.413856100937728 0.0 70-71 0.0 0.0 0.0 8.962885783747009 0.0 72-73 0.0 0.0 0.0 9.495025905256696 0.0 74-75 0.0 0.0 0.0 10.03454034226339 0.0 76-77 0.0 0.0 0.0 10.564301652322435 0.0 78-79 0.0 0.0 0.0 11.203250407966163 0.0 80-81 0.0 0.0 0.0 11.838630946433923 0.0 82-83 0.0 0.0 0.0 12.365299801607126 0.0 84-85 0.0 0.0 0.0 12.922417443348605 0.0 86-87 0.0 0.0 0.0 13.48429270799138 0.0 88-89 0.0 0.0 0.0 14.088035054165537 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTGAG 25 0.005019413 56.989006 9 GGGAGCG 25 0.005019413 56.989006 7 TAATACC 45 9.6162246E-4 42.21408 4 AGGGCGT 45 9.6162246E-4 42.21408 6 CTAAGGA 55 0.0025772874 34.538795 4 GTAAGCG 50 1.2961373E-6 33.2515 94-95 TCTAGTA 60 0.0039428007 31.668095 3 ACTTAGA 60 0.0039428007 31.668095 1 ACTAAGG 60 0.0039428007 31.668095 3 GGTTTGC 60 0.003947398 31.66056 8 CATGGGT 125 2.1433698E-7 30.39414 4 CATGAGA 65 0.005838852 29.225134 4 CATTCCG 65 0.005838852 29.225134 9 TTGAGTA 70 0.008374403 27.14408 2 ATGAGAG 70 0.008384134 27.137623 5 TAAGGAG 70 0.008384134 27.137623 5 ATGGGTA 100 0.0016510204 23.74542 5 GAGTACT 3190 0.0 23.373234 12-13 GTACATG 2225 0.0 22.203249 1 GTACTTT 3275 0.0 22.114056 14-15 >>END_MODULE