FastQCFastQC Report
Thu 26 May 2016
SRR938039_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938039_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1830304
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165720.9054233613651066No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136810.7474714582932671No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT118580.6478705176844939No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92080.5030858261796947No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT70520.38529118660069583No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51970.2839419025473364No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA41110.2246074968966904No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA37100.2026985681067189No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC37060.2024800251761456No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35490.19390221515114428No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34750.18985917093553856No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA33600.1835760616815567No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT32500.17756613109079147No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC31450.17182937916324284No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC30850.1685512352046436No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA30640.16740388481913387No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGT30280.16543699844397433No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT30050.16418037659317797No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA29210.15959097505113903No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28730.15696845988425967No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28480.15560256656817667No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG28420.15527475217231673No Hit
GTATTAAGGCGGAAGATTTAAAAAAATTTTTGCAGTACATAATTTACACA27770.15172342955050092No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA27310.14921018584890816No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG27280.1490462786509782No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC26980.1474072066716786No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG26880.14686084934524538No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT26830.14658767068202877No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA23660.1292681434340962No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT22970.1254982778817071No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG22700.12402311310033742No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA22040.12041715474587827No Hit
GGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGAAGGCT21720.11866881130129202No Hit
GGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCT20820.11375159536339317No Hit
TCTTAGGAGTGGGGGTGGCTTTTGGGAGGGTGAGGGACTTCCTGTAACCA20490.11194861618616361No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19990.10921682955399759No Hit
CCTCAACCCCCTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGG19780.10806947916848786No Hit
ATACACATCTCCGAGCCCACGAGACGCTACGCTATCTCGTATGCCGTCTT18820.1028244488347291RNA PCR Primer, Index 31 (95% over 22bp)
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA18770.1025512701715125No Hit
GGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCCCTGGCTG18500.10107610539014285No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTAAG11400.030.8765162
TAAGGTG14650.029.54245
GTAAGGT14800.027.9583444
TAAGGTA5800.027.0607514
AAGGTAA13050.026.6080951
GTACATG91450.025.6947651
GGTAAGG15750.025.3660353
TACATGG91650.025.015892
ACATGGG89850.023.555693
GGTGTGC18150.023.5512688
GAGTACT118650.023.33722512-13
GTGTAAG5950.023.1836831
GTACTTT121450.022.5645514-15
AAGGTGT19450.022.4655286
AGGTGTG19200.022.0159367
CGTATAG1353.7614122E-421.1406781
AGTACTT119600.021.12656412-13
CATGGGA50900.021.0241584
AGAGTAC167050.020.86882410-11
GTGTGCA20150.020.2708449
Produced by FastQC (version 0.11.5)